Rroxscaffold_6G00430650

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001834
Physical Location & Seq
Forward (+)
50769013 .. 50770497
1485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430650.1

Sequence Viewer

Length: 771 bp
ATGGCTGCTGCAATATCATCTATTGAAAAACCACTTGCACCACCATCTCCTTCTCCTACAATGGTCAAGCGAGGCTCGAACCACCCTGTTCACCAGACCCTGGCCTGTGCAGCCAATCTAGCTAACCTCCTCCCTACCGGCACTGTCTTTGCATTTCAAACCCTAACTCCGATTTTCTCCAAAGACGGATGTTGCCAACAACTTTCCAATAAGTTCCTTACTTCATCTGTCATAATAACTTGTGCTGTCCTCTGTTTCTCATCTTCTTTTACTGACAGCTTCATAGACAATGGTGGAAAACGCTATTATGGAATCTCCACTTTCAAAGGCATGTACATCTTTAACTACAATAACATGGAGGAGGATATGAATAAGTACTTGGAAAAGTATAGAATAAAGTTCATAGATTTTGTTCATGCCTTCATCTCACTAATGTTCTTCCTGATCTTTGCTCTTACCAACTCCAACGTACAAAGTTGTTTCTTTTCTGGAGGGGGAATAAATACCAGCGAACTCGCAATGAACTTGCCACTAGGTGCAGGGGTTTTATCAACCTTGTTGTTCACCATCTTCCCAACTACTCGAAGAGGAATCGGATACGCAGACATGAATCGGAAATTTGTGAAATTACATGAAGAAGAAGAAGAACTCTATAATTACCAGGATGCGATTCTAAGCCTCGGGATGATCAAAACGCGTATTCTCTATGACTGCGGTTCCATTGATTACGATCTCCTTTGTGGCCTCGATGCTTGTGCTCAGTTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

256

Amino Acids

28.46

Weight (kDa)

6.07

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 37 - 200 3.6e-57 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 100
AccII CGCG 1 cut(s) 697
AciI CCGC 1 cut(s) 714
AcsI RAATTY 1 cut(s) 617
AdeI CACNNNGTG 1 cut(s) 536
AfaI GTAC 3 cut(s) 335, 377, 471
AfiI CCNNNNNNNGG 1 cut(s) 100
AflIII ACRYGT 1 cut(s) 695
AgsI TTSAA 3 cut(s) 26, 158, 325
AjnI CCWGG 2 cut(s) 99, 660
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AluBI AGCT 2 cut(s) 122, 279
AluI AGCT 2 cut(s) 122, 279
Alw21I GWGCWC 1 cut(s) 760
AlwNI CAGNNNCTG 1 cut(s) 100
Ama87I CYCGRG 1 cut(s) 680
AoxI GGCC 2 cut(s) 102, 742
ApeKI GCWGC 3 cut(s) 5, 8, 110
ApoI RAATTY 1 cut(s) 617
AsuHPI GGTGA 2 cut(s) 83, 556
AvaI CYCGRG 1 cut(s) 680
Bbv12I GWGCWC 1 cut(s) 760
BbvI GCAGC 1 cut(s) 122
BccI CCATC 2 cut(s) 52, 575
BcgI CGANNNNNNTGC 2 cut(s) 737, 771
BciT130I CCWGG 2 cut(s) 101, 662
BciVI GTATCC 1 cut(s) 590
BclI TGATCA 1 cut(s) 687
BfaI CTAG 3 cut(s) 119, 533, 769
BfuI GTATCC 1 cut(s) 590
BisI GCNGC 3 cut(s) 6, 9, 111
BlsI GCNGC 3 cut(s) 7, 10, 112
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 2 cut(s) 101, 662
BmeT110I CYCGRG 1 cut(s) 680
BmiI GGNNCC 1 cut(s) 718
BmrFI CCNGG 2 cut(s) 101, 662
BmsI GCATC 2 cut(s) 655, 739
BpmI CTGGAG 1 cut(s) 510
BsaBI GATNNNNATC 1 cut(s) 729
BsaJI CCNNGG 2 cut(s) 99, 679
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse118I RCCGGY 1 cut(s) 137
Bse3DI GCAATG 1 cut(s) 525
Bse8I GATNNNNATC 1 cut(s) 729
BseBI CCWGG 2 cut(s) 101, 662
BseDI CCNNGG 2 cut(s) 99, 679
BseGI GGATG 3 cut(s) 194, 670, 690
BseJI GATNNNNATC 1 cut(s) 729
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMI GCAATG 1 cut(s) 525
BseRI GAGGAG 2 cut(s) 119, 374
BseXI GCAGC 1 cut(s) 122
BsgI GTGCAG 2 cut(s) 129, 558
Bsh1236I CGCG 1 cut(s) 697
BshFI GGCC 2 cut(s) 104, 744
BsiHKAI GWGCWC 1 cut(s) 760
BsiHKCI CYCGRG 1 cut(s) 680
BsiSI CCGG 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 100
BsnI GGCC 2 cut(s) 104, 744
BsoBI CYCGRG 1 cut(s) 680
Bsp1286I GDGCHC 1 cut(s) 760
Bsp1407I TGTACA 1 cut(s) 333
Bsp143I GATC 3 cut(s) 444, 687, 730
BspACI CCGC 1 cut(s) 714
BspANI GGCC 2 cut(s) 104, 744
BspFNI CGCG 1 cut(s) 697
BspLI GGNNCC 1 cut(s) 718
BsrDI GCAATG 1 cut(s) 525
BsrFI RCCGGY 1 cut(s) 137
BsrGI TGTACA 1 cut(s) 333
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 2 cut(s) 99, 679
BssMI GATC 3 cut(s) 444, 687, 730
Bst2UI CCWGG 2 cut(s) 101, 662
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 1 cut(s) 580
BstAUI TGTACA 1 cut(s) 333
BstDEI CTNAG 2 cut(s) 674, 759
BstF5I GGATG 3 cut(s) 194, 670, 690
BstFNI CGCG 1 cut(s) 697
BstKTI GATC 3 cut(s) 447, 690, 733
BstMBI GATC 3 cut(s) 444, 687, 730
BstMWI GCNNNNNNNGC 2 cut(s) 110, 119
BstNI CCWGG 2 cut(s) 101, 662
BstNSI RCATGY 1 cut(s) 334
BstSCI CCNGG 2 cut(s) 99, 660
BstUI CGCG 1 cut(s) 697
BstV1I GCAGC 1 cut(s) 122
BsuI GTATCC 1 cut(s) 590
BsuRI GGCC 2 cut(s) 104, 744
BtsCI GGATG 3 cut(s) 194, 670, 690
BtsIMutI CAGTG 1 cut(s) 141
CaiI CAGNNNCTG 1 cut(s) 100
Cfr10I RCCGGY 1 cut(s) 137
Csp6I GTAC 3 cut(s) 334, 376, 470
CviAII CATG 5 cut(s) 331, 355, 416, 607, 632
CviJI RGCY 8 cut(s) 5, 75, 104, 113, 122, 279, 678, 744
CviKI_1 RGCY 8 cut(s) 5, 75, 104, 113, 122, 279, 678, 744
CviQI GTAC 3 cut(s) 334, 376, 470
DdeI CTNAG 2 cut(s) 674, 759
DpnI GATC 3 cut(s) 446, 689, 732
DpnII GATC 3 cut(s) 444, 687, 730
DraIII CACNNNGTG 1 cut(s) 536
Eam1104I CTCTTC 1 cut(s) 580
EarI CTCTTC 1 cut(s) 580
Eco88I CYCGRG 1 cut(s) 680
EcoRII CCWGG 2 cut(s) 99, 660
FaeI CATG 5 cut(s) 334, 358, 419, 610, 635
FatI CATG 5 cut(s) 330, 354, 415, 606, 631
FbaI TGATCA 1 cut(s) 687
Fnu4HI GCNGC 3 cut(s) 6, 9, 111
FokI GGATG 3 cut(s) 201, 677, 697
Fsp4HI GCNGC 3 cut(s) 6, 9, 111
FspBI CTAG 3 cut(s) 119, 533, 769
GluI GCNGC 3 cut(s) 6, 9, 111
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 2 cut(s) 104, 744
HapII CCGG 1 cut(s) 138
Hin1II CATG 5 cut(s) 334, 358, 419, 610, 635
HinfI GANTC 4 cut(s) 312, 591, 610, 670
HpaII CCGG 1 cut(s) 138
HphI GGTGA 2 cut(s) 83, 556
Hpy166II GTNNAC 2 cut(s) 91, 564
Hpy188I TCNGA 3 cut(s) 171, 596, 615
Hpy188III TCNNGA 3 cut(s) 442, 489, 682
Hpy8I GTNNAC 2 cut(s) 91, 564
HpyAV CCTTC 2 cut(s) 60, 430
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 1 cut(s) 468
HpyCH4V TGCA 5 cut(s) 11, 38, 110, 152, 539
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 119
HpyF3I CTNAG 2 cut(s) 674, 759
HpySE526I ACGT 1 cut(s) 468
Hsp92II CATG 5 cut(s) 334, 358, 419, 610, 635
Ksp22I TGATCA 1 cut(s) 687
Kzo9I GATC 3 cut(s) 444, 687, 730
Lsp1109I GCAGC 1 cut(s) 122
LweI GCATC 2 cut(s) 655, 739
MaeI CTAG 3 cut(s) 119, 533, 769
MaeII ACGT 1 cut(s) 468
MalI GATC 3 cut(s) 446, 689, 732
MboI GATC 3 cut(s) 444, 687, 730
MboII GAAGA 9 cut(s) 255, 430, 562, 597, 647, 650, 653, 656, 757
MhlI GDGCHC 1 cut(s) 760
MluCI AATT 3 cut(s) 617, 626, 655
MluI ACGCGT 1 cut(s) 695
MmeI TCCRAC 1 cut(s) 489
MseI TTAA 1 cut(s) 342
MspI CCGG 1 cut(s) 138
MspR9I CCNGG 2 cut(s) 101, 662
MvaI CCWGG 2 cut(s) 101, 662
MvnI CGCG 1 cut(s) 697
MwoI GCNNNNNNNGC 2 cut(s) 110, 119
NdeII GATC 3 cut(s) 444, 687, 730
NlaIII CATG 5 cut(s) 334, 358, 419, 610, 635
NlaIV GGNNCC 1 cut(s) 718
NspI RCATGY 1 cut(s) 334
PfeI GAWTC 4 cut(s) 312, 591, 610, 670
PflMI CCANNNNNTGG 1 cut(s) 100
PkrI GCNGC 3 cut(s) 7, 10, 112
Psp6I CCWGG 2 cut(s) 99, 660
PspGI CCWGG 2 cut(s) 99, 660
PspN4I GGNNCC 1 cut(s) 718
PstNI CAGNNNCTG 1 cut(s) 100
RsaI GTAC 3 cut(s) 335, 377, 471
RsaNI GTAC 3 cut(s) 334, 376, 470
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 3 cut(s) 6, 9, 111
Sau3AI GATC 3 cut(s) 444, 687, 730
ScaI AGTACT 1 cut(s) 377
ScrFI CCNGG 2 cut(s) 101, 662
SduI GDGCHC 1 cut(s) 760
SetI ASST 6 cut(s) 124, 129, 281, 471, 538, 557
SfaNI GCATC 2 cut(s) 655, 739
Sse9I AATT 3 cut(s) 617, 626, 655
SsiI CCGC 1 cut(s) 714
SspMI CTAG 3 cut(s) 119, 533, 769
StyD4I CCNGG 2 cut(s) 99, 660
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 1 cut(s) 471
TaqI TCGA 3 cut(s) 77, 583, 747
TasI AATT 3 cut(s) 617, 626, 655
TatI WGTACW 2 cut(s) 333, 375
TfiI GAWTC 4 cut(s) 312, 591, 610, 670
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 1 cut(s) 148
TseI GCWGC 3 cut(s) 5, 8, 110
TspDTI ATGAA 9 cut(s) 213, 271, 383, 391, 404, 412, 536, 623, 648
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 1 cut(s) 148
Van91I CCANNNNNTGG 1 cut(s) 100
XapI RAATTY 1 cut(s) 617
XceI RCATGY 1 cut(s) 334
XspI CTAG 3 cut(s) 119, 533, 769
ZrmI AGTACT 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.