Rroxscaffold_6G00430680

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001834
Physical Location & Seq
Forward (+)
50781187 .. 50781903
717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430680.1

Sequence Viewer

Length: 717 bp
ATGACTATTGCAACACAAGACAAAAACGTATCAGCCACCCAAAAGACCATAGCCAGTGCAGCCAATCTTGCTAACCTCCTACCTACCGGCACCCTTCTCGCATTTGAGACCCTCATTCCTAGTTTCTCCAACAACGGAGCATGCCATCGGGCCAACAAGGGCCTTTCCTCATCCATCATTCTAATTTGTGCTCTCATCTGCTTCTTCTCCTCCTTTACTGATAGCTTCATTGATAGTACCGATGGAAAATTCTATTATGGGATCGCCACTAGCACAGGCATACGCATCTTAAATCCTAGAACAAAAAAGATCACTTATGAGGAAAGAAAACGCTTGGAAAAGTATAAAATCACGGGTAGAGATTATTTCCATGCCTTTTTGGCACTAACTGTGTTCTTGGTCTTTGCTCTTACCGACCTCAATGTGAAAAATTGTTTCTTTCCTGATGCGGGAGATGACCTCAATCAAATCATTATCAACTTGCCACTGGGAGCTGGGATTTTCGCATCCTTCTTATTCACTATTTTTCCAACTACTCGAAGAGGGATAGGATACACGCAGCCCCGGCCGAATAAGGAGGAAAAAGACGGGAACCAGACCTCGGCCATTACGACTGCTTCCAAGGGGTCGGAGGCGGAAATGGTGGGCGTAGTCGTAGAAGGAGCAGTTCAGTCTGAGGCTCAGGTCTCAGCGACAGCCTCAACCCCACCGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

238

Amino Acids

25.79

Weight (kDa)

8.64

Isoelectric Point (pI)

27.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 19 - 185 4.3e-59 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 89
AccII CGCG 1 cut(s) 712
AciI CCGC 3 cut(s) 449, 635, 710
AclWI GGATC 1 cut(s) 269
AcoI YGGCCR 2 cut(s) 566, 603
AcsI RAATTY 1 cut(s) 248
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 2 cut(s) 449, 601
AluBI AGCT 2 cut(s) 225, 494
AluI AGCT 2 cut(s) 225, 494
Alw21I GWGCWC 1 cut(s) 193
Alw26I GTCTC 2 cut(s) 101, 691
AlwI GGATC 1 cut(s) 269
AoxI GGCC 4 cut(s) 150, 160, 566, 603
ApeKI GCWGC 2 cut(s) 59, 559
ApoI RAATTY 1 cut(s) 248
AspS9I GGNCC 2 cut(s) 150, 160
AsuC2I CCSGG 1 cut(s) 565
BanI GGYRCC 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 2 cut(s) 71, 571
BccI CCATC 3 cut(s) 153, 182, 236
BcgI CGANNNNNNTGC 2 cut(s) 79, 113
BciVI GTATCC 1 cut(s) 545
BcnI CCSGG 1 cut(s) 565
BcoDI GTCTC 2 cut(s) 101, 691
BfaI CTAG 3 cut(s) 120, 270, 297
BfuI GTATCC 1 cut(s) 545
BglI GCCNNNNNGGC 1 cut(s) 380
BisI GCNGC 2 cut(s) 60, 560
BlsI GCNGC 2 cut(s) 61, 561
Bme1390I CCNGG 1 cut(s) 565
BmgT120I GGNCC 2 cut(s) 150, 160
BmiI GGNNCC 2 cut(s) 91, 593
BmrFI CCNGG 1 cut(s) 565
BmrI ACTGGG 1 cut(s) 497
BmsI GCATC 3 cut(s) 294, 436, 515
BmuI ACTGGG 1 cut(s) 497
BplI GAGNNNNNCTC 2 cut(s) 444, 476
Bpu10I CCTNAGC 1 cut(s) 681
BpuMI CCSGG 1 cut(s) 565
BsaI GGTCTC 2 cut(s) 101, 691
BsaJI CCNNGG 3 cut(s) 563, 600, 621
Bsc4I CCNNNNNNNGG 2 cut(s) 449, 601
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 54, 492
BseDI CCNNGG 3 cut(s) 563, 600, 621
BseGI GGATG 2 cut(s) 170, 506
BseLI CCNNNNNNNGG 2 cut(s) 449, 601
BseMII CTCAG 3 cut(s) 666, 695, 702
BseNI ACTGG 2 cut(s) 54, 492
BseRI GAGGAG 1 cut(s) 199
BseX3I CGGCCG 1 cut(s) 566
BseXI GCAGC 2 cut(s) 71, 571
BseYI CCCAGC 1 cut(s) 494
BsgI GTGCAG 1 cut(s) 78
Bsh1236I CGCG 1 cut(s) 712
Bsh1285I CGRYCG 1 cut(s) 569
BshFI GGCC 4 cut(s) 152, 162, 568, 605
BshNI GGYRCC 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 569
BsiHKAI GWGCWC 1 cut(s) 193
BsiSI CCGG 2 cut(s) 87, 565
BslI CCNNNNNNNGG 2 cut(s) 449, 601
BsmAI GTCTC 2 cut(s) 101, 691
BsnI GGCC 4 cut(s) 152, 162, 568, 605
Bso31I GGTCTC 2 cut(s) 101, 691
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 2 cut(s) 261, 309
BspACI CCGC 3 cut(s) 449, 635, 710
BspANI GGCC 4 cut(s) 152, 162, 568, 605
BspCNI CTCAG 3 cut(s) 667, 694, 701
BspFNI CGCG 1 cut(s) 712
BspLI GGNNCC 2 cut(s) 91, 593
BspPI GGATC 1 cut(s) 269
BspT107I GGYRCC 1 cut(s) 89
BspTNI GGTCTC 2 cut(s) 101, 691
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 2 cut(s) 54, 492
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 3 cut(s) 563, 600, 621
BssMI GATC 2 cut(s) 261, 309
BssT1I CCWWGG 1 cut(s) 621
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 1 cut(s) 535
BstC8I GCNNGC 1 cut(s) 142
BstDEI CTNAG 3 cut(s) 675, 681, 688
BstF5I GGATG 2 cut(s) 170, 506
BstFNI CGCG 1 cut(s) 712
BstKTI GATC 2 cut(s) 264, 312
BstMAI GTCTC 2 cut(s) 101, 691
BstMBI GATC 2 cut(s) 261, 309
BstMCI CGRYCG 1 cut(s) 569
BstMWI GCNNNNNNNGC 4 cut(s) 59, 68, 380, 565
BstNSI RCATGY 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 563
BstUI CGCG 1 cut(s) 712
BstV1I GCAGC 2 cut(s) 71, 571
BstZI CGGCCG 1 cut(s) 566
BsuI GTATCC 1 cut(s) 545
BsuRI GGCC 4 cut(s) 152, 162, 568, 605
BtsCI GGATG 2 cut(s) 170, 506
BtsIMutI CAGTG 2 cut(s) 61, 485
Cac8I GCNNGC 1 cut(s) 142
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 2 cut(s) 150, 160
Csp6I GTAC 1 cut(s) 237
CviAII CATG 2 cut(s) 141, 371
CviQI GTAC 1 cut(s) 237
DdeI CTNAG 3 cut(s) 675, 681, 688
DpnI GATC 2 cut(s) 263, 311
DpnII GATC 2 cut(s) 261, 309
EaeI YGGCCR 2 cut(s) 566, 603
EagI CGGCCG 1 cut(s) 566
Eam1104I CTCTTC 1 cut(s) 535
EarI CTCTTC 1 cut(s) 535
EciI GGCGGA 1 cut(s) 650
EclXI CGGCCG 1 cut(s) 566
Eco130I CCWWGG 1 cut(s) 621
Eco31I GGTCTC 2 cut(s) 101, 691
Eco52I CGGCCG 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 160
EcoT14I CCWWGG 1 cut(s) 621
ErhI CCWWGG 1 cut(s) 621
FaeI CATG 2 cut(s) 144, 374
FaiI YATR 7 cut(s) 50, 142, 258, 281, 318, 345, 372
FatI CATG 2 cut(s) 140, 370
FauI CCCGC 1 cut(s) 442
Fnu4HI GCNGC 2 cut(s) 60, 560
FokI GGATG 2 cut(s) 157, 493
Fsp4HI GCNGC 2 cut(s) 60, 560
FspBI CTAG 3 cut(s) 120, 270, 297
GluI GCNGC 2 cut(s) 60, 560
GsaI CCCAGC 1 cut(s) 498
HaeIII GGCC 4 cut(s) 152, 162, 568, 605
HapII CCGG 2 cut(s) 87, 565
Hin1II CATG 2 cut(s) 144, 374
HpaII CCGG 2 cut(s) 87, 565
Hpy188I TCNGA 2 cut(s) 631, 676
Hpy188III TCNNGA 1 cut(s) 443
HpyAV CCTTC 3 cut(s) 104, 520, 653
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 2 cut(s) 11, 59
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 68, 380, 565
HpyF3I CTNAG 3 cut(s) 675, 681, 688
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 2 cut(s) 144, 374
Kzo9I GATC 2 cut(s) 261, 309
LmnI GCTCC 3 cut(s) 137, 491, 662
LpnPI CCDG 9 cut(s) 67, 100, 261, 456, 473, 480, 578, 608, 668
Lsp1109I GCAGC 2 cut(s) 71, 571
LweI GCATC 3 cut(s) 294, 436, 515
MaeI CTAG 3 cut(s) 120, 270, 297
MaeII ACGT 1 cut(s) 27
MalI GATC 2 cut(s) 263, 311
MboI GATC 2 cut(s) 261, 309
MboII GAAGA 2 cut(s) 196, 552
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 3 cut(s) 183, 248, 430
MmeI TCCRAC 3 cut(s) 153, 554, 609
MseI TTAA 1 cut(s) 290
MspI CCGG 2 cut(s) 87, 565
MspR9I CCNGG 1 cut(s) 565
MvnI CGCG 1 cut(s) 712
MwoI GCNNNNNNNGC 4 cut(s) 59, 68, 380, 565
NciI CCSGG 1 cut(s) 565
NdeII GATC 2 cut(s) 261, 309
NlaIII CATG 2 cut(s) 144, 374
NlaIV GGNNCC 2 cut(s) 91, 593
NmeAIII GCCGAG 1 cut(s) 581
NspI RCATGY 1 cut(s) 144
PaeI GCATGC 1 cut(s) 144
PcsI WCGNNNNNNNCGW 1 cut(s) 608
PkrI GCNGC 2 cut(s) 61, 561
PspFI CCCAGC 1 cut(s) 494
PspN4I GGNNCC 2 cut(s) 91, 593
PspPI GGNCC 2 cut(s) 150, 160
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
SaqAI TTAA 1 cut(s) 290
SatI GCNGC 2 cut(s) 60, 560
Sau3AI GATC 2 cut(s) 261, 309
Sau96I GGNCC 2 cut(s) 150, 160
ScrFI CCNGG 1 cut(s) 565
SduI GDGCHC 1 cut(s) 193
SetI ASST 9 cut(s) 30, 78, 85, 227, 420, 462, 496, 602, 687
SfaNI GCATC 3 cut(s) 294, 436, 515
SphI GCATGC 1 cut(s) 144
Sse9I AATT 3 cut(s) 183, 248, 430
SsiI CCGC 3 cut(s) 449, 635, 710
SspMI CTAG 3 cut(s) 120, 270, 297
StyD4I CCNGG 1 cut(s) 563
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 1 cut(s) 391
TaiI ACGT 1 cut(s) 30
TaqI TCGA 1 cut(s) 538
TasI AATT 3 cut(s) 183, 248, 430
Tru1I TTAA 1 cut(s) 290
Tru9I TTAA 1 cut(s) 290
TscAI CASTG 2 cut(s) 61, 492
TseI GCWGC 2 cut(s) 59, 559
TspDTI ATGAA 1 cut(s) 217
TspGWI ACGGA 1 cut(s) 150
TspRI CASTG 2 cut(s) 61, 492
XapI RAATTY 1 cut(s) 248
XceI RCATGY 1 cut(s) 144
XspI CTAG 3 cut(s) 120, 270, 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.