Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002974

Physical Location & Seq

Genomic Coordinates Forward (+)

22825713 .. 22828818

Gene Structure 3106 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1111200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1743 bp

ATGAAAAGTTATTCCCCAACAAGCAGCCCGCCGCTCTTTTTCCCCCTTCTTTCCTCTTCCTCTGGTTCCCTGCCTCACCTTCTCTTTTGCTGCTGCCTTTCTTCTTCTTCCCTCTTCAGCTCTTCACTTCCAAATCCCACAGCAGTCTTGGGGTTTGAGGCTTTTTCTCTGTTGGGTTTTCTTCCAGTAGCTCAAAGCTCCAAAAGATACTTGTCAATTATCCGAAGCTGGATATTGAGGTCTTTAAGCGTAGGGTTCACCATGAACAACCTCTCTGCTTTCAGAGCCCTGGACTCCAGTCCACTCTCCAGCCCTCGCTCCTTTGGGCCCCACAACTACATACCTATTCTCCTCCAGCGCCCGAGGAATGCTGTCGTATGCTCAGTGAAACAATCAATTGCCACCCCTTCACCTCTAAGTGTTGATAGTTCCCCTAACCAGGGGCTTAATCGAATCGAATCACTAACCCAGGTTTCGGGGGTGTTGGGCTGCCAATGGGGTGATGAAGGTAAAGGAAAACTTGTTGACATCTTGGCCCAGCATTTTGACATCGTTGCTCGTTGTCAGGGTGGAGCTAATGCTGGACACACCATTTACAATGCAGAGGGAAAGAAGTTTGCACTTCACCTTGTTCCGTCGGGTATTCTTAATGAAGAAACTCTTTGTGTTATTGGGAACGGAGTTGTGGTGCATCTTCCAGGGCTCTTTAAAGAGATTGATGGCCTTGAATCTAATGGAGTCTCCTGCAAGGGAAGGATATTGGTCTCTGATCGTGCTCACCTGTTATTTGATTTCCACCAAGTAGTGGATGGACTTCGAGAATCTGAGCTTGCTAAATCTTTCATTGGCACTACCAAGAGGGGCATTGGACCATGTTACTCTAGCAAGGTTAACCGAAATGGTATTCGAGTGAGTGACTTGAGGTACATGGATACTTTTCCTCAGAAGCTAGATCTTCTATTATCAGATGCTGCATCAAGGTTCCAAGGTTTTGAGTATGGGCCCCATGTGCTCAAAGAAGAAGTTGAAAAATACAAGAGATTTGCTGAGAGATTGGAACCCTTCATTGCAGATACTGTGCTTGTTGTGAATGAAGCCATCTCTGAGAAGAAAAAGATTTTGGTCGAAGGTGGTCAGGCAACAATGTTGGATATTGACTTTGGAACTTACCCCTTTGTAACATCCTCTAGCCCATCAGCCGGTGGGATTTGCACTGGTCTCGGAATTGCTCCCAGAGTTTTGGGTGATCTGGTTGGAGTGGTGAAAGCGTACACTACAAGAGTTGGTTCTGGTCCCTTTCCCACAGAAATCTTGGGTCAAGGGGGTGATCTTCTTAGGTTTGCTGGTCAGGAGTTTGGCACAACTACTGGCCGCCCTCGTCGTTGTGGTTGGCTTGATATAGCAGCTCTGAAGTATTGCTGTCAGATTAATGGCTTTTCTTCACTCAATCTCACCAAACTTGATGTTTTGTCTGATCTTCCTGAAATTCAGTTGGGTGTTGCCTACAAACAGACTGATGGTACACCAGTCAAATCATTCCCTGCAGACCTTCTTGATCTGGAGCAATTAAAGGTGGACTACGAAGTATTGCCCGGATGGAAGTCAGATATTTCGTCAGTCAGAAACTACTCTGACCTTCCAAAGGCTGCACGTCAGTACGTGGAAAGGATAGAAGAACTTGTTGGTGTACCCGTCCATTACATTGGTGTGGGGCCTGGACGCGATGCACTCATTTACAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004019 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006163 GO:0006164 GO:0006167 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010035 GO:0010038 GO:0016874 GO:0016879 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0034641 GO:0034654 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044208 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046033 GO:0046040 GO:0046390 GO:0046483 GO:0046686 GO:0048046 GO:0050896 GO:0055086 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00230 ko00250 ko01100 map00230 map00250 map01100

Pfam Domains

Protein Families

Adenylsucc_synt

Protein Analysis

581

Amino Acids

62.85

Weight (kDa)

7.88

Isoelectric Point (pI)

45.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Adenylsucc_synt	PF00709	161 - 578	6.5e-176	Adenylosuccinate synthetase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002974)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57610
fragaria_vesca	FvH4_2g02690 FvH4_7g07940
malus_domestica	MD00G1080700.v1.1 MD05G1111200.v1.1 MD15G1386600.v1.1
prunus_persica	Prupe.5G015200_v2.0.a1 Prupe.5G015200_v2.0.a1 Prupe.5G015200_v2.0.a1 Prupe.5G015200_v2.0.a1 Prupe.8G156300_v2.0.a1 Prupe.8G156300_v2.0.a1 Prupe.8G156300_v2.0.a1
pyrus_communis	pycom05g10700 pycom10g09910 pycom15g34650
rosa_chinensis	RchiOBHm_Chr6g0247091 RchiOBHm_Chr6g0247101 RchiOBHm_Chr7g0177111 RchiOBHm_Chr7g0177151
rosa_laevigata	RLG00000015220 RLG00000030365
rosa_multiflora	Rmu_sc0005310.1_g000020 Rmu_sc0010391.1_g000001 Rmu_sc0012524.1_g000001
rosa_roxburghii	Rroxscaffold_3G00276060 Rroxscaffold_7G00214350
rosa_rugosa	Rorug05G0522100 Rorug05G0522200 Rorug05G0522300
rosa_samantha	Rh6AG037300 Rh6BG032200 Rh6CG033300 Rh6DG031000 Rh7AG003500 Rh7CG003200
rosa_wichuraiana	Rw6G003200 Rw7G000290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	805
AccBSI	CCGCTC	1 cut(s)	34
AccII	CGCG	1 cut(s)	1722
AciI	CCGC	3 cut(s)	29, 32, 1372
AcoI	YGGCCR	1 cut(s)	1369
AcsI	RAATTY	1 cut(s)	1485
AcuI	CTGAAG	2 cut(s)	100, 1430
AfaI	GTAC	5 cut(s)	926, 1271, 1522, 1658, 1689
AfiI	CCNNNNNNNGG	6 cut(s)	439, 440, 475, 805, 1199, 1642
AgsI	TTSAA	2 cut(s)	728, 1028
AjiI	CACGTC	1 cut(s)	1652
AjnI	CCWGG	5 cut(s)	288, 438, 468, 697, 1714
AjuI	GAANNNNNNNTTGG	4 cut(s)	1103, 1135, 1665, 1697
AluBI	AGCT	8 cut(s)	120, 191, 198, 228, 575, 829, 949, 1406
AluI	AGCT	8 cut(s)	120, 191, 198, 228, 575, 829, 949, 1406
Alw21I	GWGCWC	2 cut(s)	778, 1014
Alw26I	GTCTC	3 cut(s)	745, 769, 1223
AlwNI	CAGNNNCTG	2 cut(s)	971, 1076
Ama87I	CYCGRG	1 cut(s)	361
AoxI	GGCC	6 cut(s)	326, 534, 721, 1001, 1369, 1712
ApaI	GGGCCC	2 cut(s)	330, 1005
ApeKI	GCWGC	7 cut(s)	24, 90, 93, 489, 971, 1403, 1646
ApoI	RAATTY	1 cut(s)	1485
AseI	ATTAAT	1 cut(s)	1428
AspLEI	GCGC	1 cut(s)	360
AspS9I	GGNCC	8 cut(s)	326, 327, 535, 869, 1001, 1002, 1292, 1712
AsuC2I	CCSGG	1 cut(s)	1593
AvaI	CYCGRG	1 cut(s)	361
AvaII	GGWCC	2 cut(s)	869, 1292
BaeGI	GKGCMC	2 cut(s)	330, 1005
BaeI	ACNNNNGTAYC	2 cut(s)	1512, 1545
BanII	GRGCYC	4 cut(s)	289, 330, 705, 1005
Bbv12I	GWGCWC	2 cut(s)	778, 1014
BbvI	GCAGC	7 cut(s)	36, 77, 80, 476, 958, 1415, 1633
BccI	CCATC	6 cut(s)	713, 803, 1106, 1201, 1511, 1590
BcgI	CGANNNNNNTGC	2 cut(s)	1201, 1235
BciT130I	CCWGG	5 cut(s)	290, 440, 470, 699, 1716
BciVI	GTATCC	1 cut(s)	925
BcnI	CCSGG	1 cut(s)	1593
BcoDI	GTCTC	3 cut(s)	745, 769, 1223
BfaI	CTAG	3 cut(s)	882, 950, 1188
BfmI	CTRYAG	1 cut(s)	1542
BfoI	RGCGCY	1 cut(s)	361
BfuI	GTATCC	1 cut(s)	925
BglII	AGATCT	1 cut(s)	952
BisI	GCNGC	9 cut(s)	25, 32, 91, 94, 490, 972, 1372, 1404, 1647
BlsI	GCNGC	9 cut(s)	26, 33, 92, 95, 491, 973, 1373, 1405, 1648
Bme1390I	CCNGG	6 cut(s)	290, 440, 470, 699, 1593, 1716
Bme18I	GGWCC	2 cut(s)	869, 1292
BmeT110I	CYCGRG	1 cut(s)	361
BmgBI	CACGTC	1 cut(s)	1652
BmgT120I	GGNCC	8 cut(s)	326, 327, 535, 869, 1001, 1002, 1292, 1712
BmiI	GGNNCC	9 cut(s)	67, 328, 329, 983, 1003, 1004, 1059, 1294, 1713
BmrFI	CCNGG	6 cut(s)	290, 440, 470, 699, 1593, 1716
BmsI	GCATC	4 cut(s)	700, 958, 983, 1714
BoxI	GACNNNNGTC	1 cut(s)	297
BpmI	CTGGAG	4 cut(s)	280, 292, 338, 1580
BpuEI	CTTGAG	1 cut(s)	940
BpuMI	CCSGG	1 cut(s)	1593
BsaAI	YACGTR	1 cut(s)	1660
BsaI	GGTCTC	2 cut(s)	769, 1223
BsaJI	CCNNGG	6 cut(s)	288, 362, 439, 468, 698, 985
BsaXI	ACNNNNNCTCC	4 cut(s)	333, 363, 672, 702
Bsc4I	CCNNNNNNNGG	6 cut(s)	439, 440, 475, 805, 1199, 1642
Bse118I	RCCGGY	1 cut(s)	1199
Bse1I	ACTGG	5 cut(s)	185, 297, 1219, 1372, 1526
Bse3DI	GCAATG	1 cut(s)	1065
BseBI	CCWGG	5 cut(s)	290, 440, 470, 699, 1716
BseDI	CCNNGG	6 cut(s)	288, 362, 439, 468, 698, 985
BseGI	GGATG	3 cut(s)	814, 1181, 1601
BseLI	CCNNNNNNNGG	6 cut(s)	439, 440, 475, 805, 1199, 1642
BseMI	GCAATG	1 cut(s)	1065
BseMII	CTCAG	5 cut(s)	396, 816, 956, 1038, 1095
BseNI	ACTGG	5 cut(s)	185, 297, 1219, 1372, 1526
BseRI	GAGGAG	1 cut(s)	341
BseSI	GKGCMC	2 cut(s)	330, 1005
BseXI	GCAGC	7 cut(s)	36, 77, 80, 476, 958, 1415, 1633
BseYI	CCCAGC	1 cut(s)	537
BsgI	GTGCAG	1 cut(s)	1632
Bsh1236I	CGCG	1 cut(s)	1722
BshFI	GGCC	6 cut(s)	328, 536, 723, 1003, 1371, 1714
BsiHKAI	GWGCWC	2 cut(s)	778, 1014
BsiHKCI	CYCGRG	1 cut(s)	361
BsiSI	CCGG	2 cut(s)	1200, 1593
BslFI	GGGAC	1 cut(s)	1278
BslI	CCNNNNNNNGG	6 cut(s)	439, 440, 475, 805, 1199, 1642
BsmAI	GTCTC	3 cut(s)	745, 769, 1223
BsmFI	GGGAC	1 cut(s)	1278
BsmI	GAATGC	1 cut(s)	373
BsnI	GGCC	6 cut(s)	328, 536, 723, 1003, 1371, 1714
Bso31I	GGTCTC	2 cut(s)	769, 1223
BsoBI	CYCGRG	1 cut(s)	361
Bsp120I	GGGCCC	2 cut(s)	326, 1001
Bsp1286I	GDGCHC	6 cut(s)	289, 330, 705, 778, 1005, 1014
Bsp143I	GATC	6 cut(s)	769, 952, 1246, 1327, 1474, 1555
BspACI	CCGC	3 cut(s)	29, 32, 1372
BspANI	GGCC	6 cut(s)	328, 536, 723, 1003, 1371, 1714
BspCNI	CTCAG	5 cut(s)	395, 817, 955, 1039, 1096
BspFNI	CGCG	1 cut(s)	1722
BspLI	GGNNCC	9 cut(s)	67, 328, 329, 983, 1003, 1004, 1059, 1294, 1713
BspMAI	CTGCAG	1 cut(s)	1546
BspQI	GCTCTTC	1 cut(s)	127
BspTNI	GGTCTC	2 cut(s)	769, 1223
BsrBI	CCGCTC	1 cut(s)	34
BsrDI	GCAATG	1 cut(s)	1065
BsrFI	RCCGGY	1 cut(s)	1199
BsrI	ACTGG	5 cut(s)	185, 297, 1219, 1372, 1526
BssAI	RCCGGY	1 cut(s)	1199
BssECI	CCNNGG	6 cut(s)	288, 362, 439, 468, 698, 985
BssMI	GATC	6 cut(s)	769, 952, 1246, 1327, 1474, 1555
BssT1I	CCWWGG	1 cut(s)	985
Bst2UI	CCWGG	5 cut(s)	290, 440, 470, 699, 1716
Bst4CI	ACNGT	1 cut(s)	1078
Bst6I	CTCTTC	3 cut(s)	61, 119, 127
BstBAI	YACGTR	1 cut(s)	1660
BstC8I	GCNNGC	2 cut(s)	29, 831
BstDEI	CTNAG	7 cut(s)	382, 416, 825, 942, 1047, 1104, 1334
BstENI	CCTNNNNNAGG	1 cut(s)	1640
BstF5I	GGATG	3 cut(s)	814, 1181, 1601
BstFNI	CGCG	1 cut(s)	1722
BstH2I	RGCGCY	1 cut(s)	361
BstHHI	GCGC	1 cut(s)	360
BstKTI	GATC	6 cut(s)	772, 955, 1249, 1330, 1477, 1558
BstMAI	GTCTC	3 cut(s)	745, 769, 1223
BstMBI	GATC	6 cut(s)	769, 952, 1246, 1327, 1474, 1555
BstMWI	GCNNNNNNNGC	2 cut(s)	284, 1009
BstNI	CCWGG	5 cut(s)	290, 440, 470, 699, 1716
BstPAI	GACNNNNGTC	1 cut(s)	297
BstSCI	CCNGG	6 cut(s)	288, 438, 468, 697, 1591, 1714
BstSFI	CTRYAG	1 cut(s)	1542
BstSLI	GKGCMC	2 cut(s)	330, 1005
BstUI	CGCG	1 cut(s)	1722
BstV1I	GCAGC	7 cut(s)	36, 77, 80, 476, 958, 1415, 1633
BstX2I	RGATCY	1 cut(s)	952
BstXI	CCANNNNNNTGG	2 cut(s)	1240, 1703
BstYI	RGATCY	1 cut(s)	952
BsuI	GTATCC	1 cut(s)	925
BsuRI	GGCC	6 cut(s)	328, 536, 723, 1003, 1371, 1714
BtgZI	GCGATG	1 cut(s)	1737
BtrI	CACGTC	1 cut(s)	1652
BtsCI	GGATG	3 cut(s)	814, 1181, 1601
BtsIMutI	CAGTG	2 cut(s)	390, 1212
Cac8I	GCNNGC	2 cut(s)	29, 831
CaiI	CAGNNNCTG	2 cut(s)	971, 1076
CfoI	GCGC	1 cut(s)	360
Cfr10I	RCCGGY	1 cut(s)	1199
Cfr13I	GGNCC	8 cut(s)	326, 327, 535, 869, 1001, 1002, 1292, 1712
CseI	GACGC	1 cut(s)	1728
Csp6I	GTAC	5 cut(s)	925, 1270, 1521, 1657, 1688
CviAII	CATG	4 cut(s)	262, 873, 928, 1007
CviQI	GTAC	5 cut(s)	925, 1270, 1521, 1657, 1688
DdeI	CTNAG	7 cut(s)	382, 416, 825, 942, 1047, 1104, 1334
DpnI	GATC	6 cut(s)	771, 954, 1248, 1329, 1476, 1557
DpnII	GATC	6 cut(s)	769, 952, 1246, 1327, 1474, 1555
DraI	TTTAAA	1 cut(s)	709
EaeI	YGGCCR	1 cut(s)	1369
Eam1104I	CTCTTC	3 cut(s)	61, 119, 127
EarI	CTCTTC	3 cut(s)	61, 119, 127
Eco130I	CCWWGG	1 cut(s)	985
Eco24I	GRGCYC	4 cut(s)	289, 330, 705, 1005
Eco31I	GGTCTC	2 cut(s)	769, 1223
Eco47I	GGWCC	2 cut(s)	869, 1292
Eco57I	CTGAAG	2 cut(s)	100, 1430
Eco88I	CYCGRG	1 cut(s)	361
EcoNI	CCTNNNNNAGG	1 cut(s)	1640
EcoO109I	RGGNCCY	3 cut(s)	327, 1002, 1712
EcoRII	CCWGG	5 cut(s)	288, 438, 468, 697, 1714
EcoT14I	CCWWGG	1 cut(s)	985
EcoT38I	GRGCYC	4 cut(s)	289, 330, 705, 1005
ErhI	CCWWGG	1 cut(s)	985
FaeI	CATG	4 cut(s)	265, 876, 931, 1010
FaiI	YATR	8 cut(s)	263, 341, 379, 874, 929, 999, 1008, 1400
FalI	AAGNNNNNCTT	4 cut(s)	504, 536, 645, 677
FaqI	GGGAC	1 cut(s)	1278
FatI	CATG	4 cut(s)	261, 872, 927, 1006
FauI	CCCGC	1 cut(s)	36
Fnu4HI	GCNGC	9 cut(s)	25, 32, 91, 94, 490, 972, 1372, 1404, 1647
FokI	GGATG	3 cut(s)	821, 1168, 1608
FriOI	GRGCYC	4 cut(s)	289, 330, 705, 1005
Fsp4HI	GCNGC	9 cut(s)	25, 32, 91, 94, 490, 972, 1372, 1404, 1647
FspBI	CTAG	3 cut(s)	882, 950, 1188
GlaI	GCGC	1 cut(s)	359
GluI	GCNGC	9 cut(s)	25, 32, 91, 94, 490, 972, 1372, 1404, 1647
GsaI	CCCAGC	1 cut(s)	541
GsuI	CTGGAG	4 cut(s)	280, 292, 338, 1580
HaeII	RGCGCY	1 cut(s)	361
HaeIII	GGCC	6 cut(s)	328, 536, 723, 1003, 1371, 1714
HapII	CCGG	2 cut(s)	1200, 1593
HgaI	GACGC	1 cut(s)	1728
HhaI	GCGC	1 cut(s)	360
Hin1II	CATG	4 cut(s)	265, 876, 931, 1010
Hin6I	GCGC	1 cut(s)	358
HinP1I	GCGC	1 cut(s)	358
HincII	GTYRAC	2 cut(s)	526, 892
HindII	GTYRAC	2 cut(s)	526, 892
HinfI	GANTC	6 cut(s)	293, 453, 458, 728, 738, 821
HpaI	GTTAAC	1 cut(s)	892
HpaII	CCGG	2 cut(s)	1200, 1593
Hpy166II	GTNNAC	8 cut(s)	258, 302, 526, 892, 1272, 1523, 1576, 1688
Hpy188III	TCNNGA	5 cut(s)	818, 1349, 1481, 1553, 1559
Hpy8I	GTNNAC	8 cut(s)	258, 302, 526, 892, 1272, 1523, 1576, 1688
Hpy99I	CGWCG	2 cut(s)	640, 1383
HpyAV	CCTTC	9 cut(s)	56, 89, 417, 500, 747, 1072, 1121, 1559, 1646
HpyCH4III	ACNGT	1 cut(s)	1078
HpyCH4IV	ACGT	2 cut(s)	1651, 1659
HpyF10VI	GCNNNNNNNGC	2 cut(s)	284, 1009
HpyF3I	CTNAG	7 cut(s)	382, 416, 825, 942, 1047, 1104, 1334
HpySE526I	ACGT	2 cut(s)	1651, 1659
Hsp92II	CATG	4 cut(s)	265, 876, 931, 1010
HspAI	GCGC	1 cut(s)	358
KspAI	GTTAAC	1 cut(s)	892
Kzo9I	GATC	6 cut(s)	769, 952, 1246, 1327, 1474, 1555
LguI	GCTCTTC	1 cut(s)	127
LmnI	GCTCC	5 cut(s)	203, 323, 572, 1234, 1561
Lsp1109I	GCAGC	7 cut(s)	36, 77, 80, 476, 958, 1415, 1633
LweI	GCATC	4 cut(s)	700, 958, 983, 1714
MaeI	CTAG	3 cut(s)	882, 950, 1188
MaeII	ACGT	2 cut(s)	1651, 1659
MaeIII	GTNAC	3 cut(s)	875, 914, 1177
MalI	GATC	6 cut(s)	771, 954, 1248, 1329, 1476, 1557
MbiI	CCGCTC	1 cut(s)	34
MboI	GATC	6 cut(s)	769, 952, 1246, 1327, 1474, 1555
MfeI	CAATTG	1 cut(s)	396
MflI	RGATCY	1 cut(s)	952
MhlI	GDGCHC	6 cut(s)	289, 330, 705, 778, 1005, 1014
MluCI	AATT	5 cut(s)	216, 396, 1224, 1485, 1565
MlyI	GAGTC	2 cut(s)	287, 747
MmeI	TCCRAC	2 cut(s)	1128, 1234
MseI	TTAA	7 cut(s)	245, 447, 648, 708, 891, 1428, 1568
MslI	CAYNNNNRTG	1 cut(s)	1706
MspI	CCGG	2 cut(s)	1200, 1593
MspR9I	CCNGG	6 cut(s)	290, 440, 470, 699, 1593, 1716
MunI	CAATTG	1 cut(s)	396
Mva1269I	GAATGC	1 cut(s)	373
MvaI	CCWGG	5 cut(s)	290, 440, 470, 699, 1716
MvnI	CGCG	1 cut(s)	1722
MwoI	GCNNNNNNNGC	2 cut(s)	284, 1009
NciI	CCSGG	1 cut(s)	1593
NdeII	GATC	6 cut(s)	769, 952, 1246, 1327, 1474, 1555
NlaIII	CATG	4 cut(s)	265, 876, 931, 1010
NlaIV	GGNNCC	9 cut(s)	67, 328, 329, 983, 1003, 1004, 1059, 1294, 1713
NmuCI	GTSAC	1 cut(s)	914
PciSI	GCTCTTC	1 cut(s)	127
PctI	GAATGC	1 cut(s)	373
PfeI	GAWTC	4 cut(s)	453, 458, 728, 821
PflMI	CCANNNNNTGG	1 cut(s)	805
PkrI	GCNGC	9 cut(s)	26, 33, 92, 95, 491, 973, 1373, 1405, 1648
PleI	GAGTC	2 cut(s)	287, 746
PpsI	GAGTC	2 cut(s)	287, 746
Ppu21I	YACGTR	1 cut(s)	1660
PshAI	GACNNNNGTC	1 cut(s)	297
PshBI	ATTAAT	1 cut(s)	1428
Psp6I	CCWGG	5 cut(s)	288, 438, 468, 697, 1714
PspFI	CCCAGC	1 cut(s)	537
PspGI	CCWGG	5 cut(s)	288, 438, 468, 697, 1714
PspN4I	GGNNCC	9 cut(s)	67, 328, 329, 983, 1003, 1004, 1059, 1294, 1713
PspOMI	GGGCCC	2 cut(s)	326, 1001
PspPI	GGNCC	8 cut(s)	326, 327, 535, 869, 1001, 1002, 1292, 1712
PstI	CTGCAG	1 cut(s)	1546
PstNI	CAGNNNCTG	2 cut(s)	971, 1076
PsuI	RGATCY	1 cut(s)	952
RsaI	GTAC	5 cut(s)	926, 1271, 1522, 1658, 1689
RsaNI	GTAC	5 cut(s)	925, 1270, 1521, 1657, 1688
RseI	CAYNNNNRTG	1 cut(s)	1706
SapI	GCTCTTC	1 cut(s)	127
SaqAI	TTAA	7 cut(s)	245, 447, 648, 708, 891, 1428, 1568
SatI	GCNGC	9 cut(s)	25, 32, 91, 94, 490, 972, 1372, 1404, 1647
Sau3AI	GATC	6 cut(s)	769, 952, 1246, 1327, 1474, 1555
Sau96I	GGNCC	8 cut(s)	326, 327, 535, 869, 1001, 1002, 1292, 1712
SchI	GAGTC	2 cut(s)	287, 747
ScrFI	CCNGG	6 cut(s)	290, 440, 470, 699, 1593, 1716
SduI	GDGCHC	6 cut(s)	289, 330, 705, 778, 1005, 1014
SfaNI	GCATC	4 cut(s)	700, 958, 983, 1714
SfcI	CTRYAG	1 cut(s)	1542
SinI	GGWCC	2 cut(s)	869, 1292
SmiMI	CAYNNNNRTG	1 cut(s)	1706
SmlI	CTYRAG	1 cut(s)	919
SmoI	CTYRAG	1 cut(s)	919
Sse9I	AATT	5 cut(s)	216, 396, 1224, 1485, 1565
SsiI	CCGC	3 cut(s)	29, 32, 1372
SspMI	CTAG	3 cut(s)	882, 950, 1188
StyD4I	CCNGG	6 cut(s)	288, 438, 468, 697, 1591, 1714
StyI	CCWWGG	1 cut(s)	985
TaaI	ACNGT	1 cut(s)	1078
TaiI	ACGT	2 cut(s)	1654, 1662
TaqI	TCGA	5 cut(s)	451, 456, 817, 907, 1125
TasI	AATT	5 cut(s)	216, 396, 1224, 1485, 1565
TauI	GCSGC	2 cut(s)	34, 1374
TfiI	GAWTC	4 cut(s)	453, 458, 728, 821
Tru1I	TTAA	7 cut(s)	245, 447, 648, 708, 891, 1428, 1568
Tru9I	TTAA	7 cut(s)	245, 447, 648, 708, 891, 1428, 1568
TscAI	CASTG	2 cut(s)	390, 1219
TseFI	GTSAC	1 cut(s)	914
TseI	GCWGC	7 cut(s)	24, 90, 93, 489, 971, 1403, 1646
Tsp45I	GTSAC	1 cut(s)	914
TspDTI	ATGAA	7 cut(s)	17, 278, 519, 666, 832, 1054, 1107
TspGWI	ACGGA	2 cut(s)	624, 693
TspRI	CASTG	2 cut(s)	390, 1219
Van91I	CCANNNNNTGG	1 cut(s)	805
VpaK11BI	GGWCC	2 cut(s)	869, 1292
VspI	ATTAAT	1 cut(s)	1428
XagI	CCTNNNNNAGG	1 cut(s)	1640
XapI	RAATTY	1 cut(s)	1485
XcmI	CCANNNNNNNNNTGG	3 cut(s)	145, 806, 1309
XspI	CTAG	3 cut(s)	882, 950, 1188

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.