Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0002974

Physical Location & Seq

Genomic Coordinates Reverse (-)

70783719 .. 70795237

Gene Structure 11519 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0522300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5712 bp

ATGGGGAAGCAGAAGGGAGACGGAGCTAGGAGCAAGGCCCGCCCTTCAAGTAGCAGTTTGGCGGCGTCGCTGTTGCCGTCGGGTTCTACTGCGGCGGTGGGCTTCGGTGGCTACGTCGGAGGCTCTCGCCTCGATGCTCCTCATTCCGGCGATGATTCAAGGCCCTTTTTGGAAGTAGATAGTGATTTGGCTCTACATTTGAAGAGGCTTGCAAGGAAAGATCCCACCACCAAGCTTAAAGCTTTGGCATCTCTATCCACACTGTTGAAGGAAAAGTCCACGAAGGACATAGTAGCGGCCATTCCACAATGGGGATTTGAATACAAAAGGCTTGTGGTGGACTACAATAGGGATGTGCGGCGAGCAACTCATGACACAATGACCATTCTTGTCACCGCCATTGGGAGAGATTTAGCGCCACAGCTGAAATCTTTGATGGGACCATGGTGGTTTTCTCAATTTGACCCAGTCTCTGAAGTTTCTCAAGCTGCAAAGCGATCATTTCAGGCAGTCTTTTCAGCTCCAGAAAAGAGACTAGATGCTTTAATTTTATGCACGGCAGAGGTTTTTATTTATTTGGAAGAAAATCTGAGGCTTACACTGCAAAATATGTCCGATAAAGGAACTGCTCTGGATGAATTGCAAGAGATGCATCAGCAGGTGATATCTTCATCATTGCTGGCATTGGCCACACTTCTTGATGTCTTGGTTTGCTTACAACTAGAAAGACCAGGCACCGAAAACATAGCTGCTCAACCAAAACATGCTTTGAAGGCTAGGGAAACTGCTATTTCTTGTGCCGACAAGTTGTTCACTGCTCATAAATATTTCTTAGACTTCTTGAAATCCCCAAGTCCTGCTATCCGTTCAGCCACATATTCTGTATTGAGTAGTTTTATAAAAAACGTCCCTCAAGCCTTTAATGAAGGAAATATGAAAACTCTTGCTGCTGCACTATTAGGTGGATTTCAGGAGAAGGATCCTGCTTGTCATTCATCAATGTGGGATGCAATTTTACTGTTTTCTAATAAATTTTCCGAAAGTTGGACTTCTGTCAATGTACAGAAAACTGTACTGAATCGGTTTTGGGATTTCCTTAGAAATAGGTGCTTTGGATCTCAACAGGTTTCTTATCCATCTTTGGTTCTATTCTTACACACCATGCCATCTAAAGCAGTCGTTGCAGAAACATTTTTTCTTGAATTCTTCAAGAACCTCTGGGCAGGAAGGAATCCATCGCATTCCTTAAATGCAGATAGAGTGGCATTTTTTCAAGCGTTACAAGAATGCTTTCTTTGGGCATTGCACAATGCCTCAAGATATTGCAATGGAGTGGATTCCATCAGTGATTTTCGAGCAACTCTTGTTAAAAATGTTCTGGTAAAGCTTTTGTGGCAAGACTACATTTTCTCTAGTAGCTCAAGAAAGGAGGAGAAAACATCCCCCGGATTATCAGTAGATTCATGTGAAAGTGATTTAGCTTCCAACAAGAAGAAAGTTGAAACATTGAATATCACGTACCCAATGAGCTACTTTAGAGAGTTGGCAAATTGCATTGTTGGGGTTCTTTCAGGCATTGATTTGCTGGAGCATGATTTGCTCTCAGCTTTTGCTGCAGAATTTCAGGAGAATTGTCAGAGTTTCTTTCAGCATGCTAGTAACTTGGAAAGAGAAAGTGAGTTTGCGGAGCGAGTTATACAGTTTATATCATTACTTGGAGAACATGCAATGCAGAATGGTCGAGGTTGGCCTTTGGCTTCTCTAGTTGGACCAATGTTGGCTAATTCCTTTGCATTGATGAGATCACATGATTCACCAAGTTGTGTGAAGATTCTAGCAGTGGCTGTTTCTGTATTTGGACCTCACAAGATAGTCCACGAACTTCGTATTCACAATACGTCTCCACATAGTTATTCTCCTGATGAGGGTGATAAAGCATTAGAGGAAGATAGATTCCTGCAAATGTTCAATGGAACTTTTGTTCCATGGTGTCTTAGTGGGAATAGTTACTCTTTAAGTGCTCGTCTTGACCTGTTACTCGCATTGCTTGATGATGAATATTTCTCTGAGCAGTGGGATAGTGTTATCAGATATGCCACTAACCTGGAGCATTCTGGATCTGCACCCTGCTCTTTAGATTCTGACCGGATAACTATCTTAGCAATGCTTTTACAGAAAGTAAGAAACGGAATCACAAACACAAAGGTAGGAGTTTCCATCAGCACAAAAATGGGCAACCCAGATCATTGGCATCATGAGCTTCTAGAATCAACTGCTGTTGCTGTTGCTCGATCCTCTCCTCCTCTCGGCGCTTCTAATTCTCAATTATTGTGTACTGTTGTTGGTGGTTCAACAAAGACCAAGCAAATTTCTCTGGTGTCAAGAAATACATTGATCCTGATATTTGAAGAGGTTTTCAAGAAGTTACTTTCTTTTATTATGGCATCCTCTTTTACTTGGGTTAGGGATGCAGGTTCTCTATTGACTGCCGGGGCAAACACTATTGGGCGAGAATTTGAAAATTCTGTGAGCGTGTTTGAGATGGCTCAATTTGCTCTTGAAGTACTTGATGGCGGCTTATATTCATTAAAGACACTTGGTGAAGAAAGTGGGTTGAGTTCAGTTATTTTAGCTGCAATTTTTCTGATTGACTGGGAGTTTCTGGTATTACTGACAACGATAGATGATGCATCTGATGATAAATCAAGGGAAAAATTGAAGGCCAGGCTGGGATTTGGTGAATCTTTTCATGCATTTAGATGTAAGATAAGTAATCAATTCTGGAAAACACTCAGCTTACACAATCGAAAGGCACTTGGAAAAAATTTGATTCAGTGTATGAGATCCGCCATCTTCAATGAAGAGGAATTAGACACAGAGAAGTTCACATCTCTGTGCTGCTTGTGGATGCTTGAAATTCTGGACTGTCTCTCTCAGGATCCCTATGAGGAACAAAATCTGCTGGACCAACTTCTCTGCCAAGGTGATAGATGGCCTTTGTGGATAGTTCCAGATTTTATCAGGCCAGAGGGAATAGTTGCCAAGGACTTCTCTATCCAAGACTTTGGCCACCGCAAGTTTATTTCATTTATTGACAAGATGATATCAGAAATTGGGATTGATAGAGTTGTTGGGGGTTATGCTAGATATACTCTCCCTCCCTCTGTGGAAGCAACAAATGAAAAGCCGACTCGATCCTGGCTTGCTGCTGAAATATTATGCTCGTGGAAATGGCCAGGAGGAAGTGCTGTAGCTTCATTCTTACCTTCATTGAGTGCCTATGCGAAGAGTAAAAATTTCTCTTCCCAGGAGAGCTTGCTAGATTTTATTTTCAACATTCTACTTGATGGCACACTTGTTCAGGGAGGATGTGCTACACAGAATTTTGTCTATCTGTGTCCTACTTCAAGTGATGAAGTGGAGGATATTGAAGAACCATTCTTAAGAGCTCTTGTAGCTTTTCTTTTAACTTTGTTTAATGATAAAATATGGGGTACCGAGAAAGCTATGGAGCTGTTTGCATTGCTTGTAAATAAACTTTATGTTGGAGAAGCAACAAATGCAAACTGTTTGAGGATTCTTCCTCTGATTGTTAATGTTCTTATTCAACCATTAAGTCAAAGAAGCATCAGATGTAACGACTCTAGCGGAGACGCTCAACATGATTCTTCTGGGGAAAATCATGTGCAAGATGTTATTGAAGGCTGGCTAAAAAAAGCTCGATCATTTCCGCCTTTGATCACGTGGTGGCAAACAGGAGAAGATATGGAAGATTGGATGCAGCTGGTTATTTCTTGTTATCCCATTAGTGCAGTAGAGGACATACAAACACCGAAGCTGGAGAGAAAGATCAGCTCGGTAGAGAGAAAGCTCCTACTTGAATTATTCCGGAAGCAGAGACATGGTGTTGGCACACCAGCTGTAATTAATCAGCTTCCAGTTGTGCAGATGTTGCTATCAAAGCTTATGGTCGTTTCAGTTGGTTATTGTTGGAAAGAATTTGATGAAGAAGATTGGGAGTTTGTGTTATCTCAGATAAGACGCTGGCTGCAGTCTATTGTTGTAATGATGGAGGAAATTGCTGAAAATGTCAATGACACTATCACTAGCAGCTTTACTTCTGATAACTTGGATGATGTCGTTGATAACCTTGGCAAAATTGTTTTCGTTTCAGATCCCTTTCCTATAGACATTGCCAAAAATGCCCTTTTATCATTTTCTCTGTCCTGTGGACCTTTCGGTCGCCGACAAGCAGAAGATGCAGATAACTTAAATCCCGTGAGAACAGAAAGATGGGACCCCATCAAAAATAGAATTTTAGAAGGCATCCTTCGATTGTTGTTCTGCACGGGTATTGCTGAGGCCATTGCAAGCTCCTGTTGTCACGAGGCTGCATTTATTGTATCAGCGTTGCGATTTGAACATTCCTATTTCTGGGAATTGGTGGCCTCAAATGTGGTCAACTCATCAACAGATGCCATAGATAGAGCAGTGAAGTCAGTTGAATTCTGGGGGCTGAGCAAAGGACCCATTAGCTCTTTGTATGCAATCCTTTTTTCTGCCAAAACAGTTCCTCTGTTGCAGTTCGCTGCTTACTTTATTCTTTCAACTGAACTTGTTTTACGCCTGGCTATTGTTGAAGAAGACAAAAGTTACTTGGATGGCGTTAGTAACAATGAAGAGGTTTCATCCTCTCTTGACATGTCGACAGAAACAGATATTCATTTGAGAGCTGAAATATCTTGCATGATTGAAAAGTTACCTAGTAATGTTCTCGAGATGGATTTAGTGGCAGATCAAAGGGTACATGTTTTTCTTGCGTGGTCCTTGTTGTTATCTCATCTAGGGTCATTGCCATCATCATCGCCTGCAAGGGAGAGACTAGTCCAGTACGTACAAGATTCTGCTAATCCAGTAATATTAGATTGCCTCTTCCAGCATATTCCTCTAGAACTGTGGATTCTTAAGAAGAAAGACGAAGAGCTTCCTGCTGGCATAGCAGAAGCTGCAGCTGCAGCAACACGTTCCATTAGAACTGGTTCCTTATTGTTTTCTGTACAGTCTCTCTGGCCTGTTGAACCACTGAAAATGGCTTCGCTTGCTGGAGCAATGTTTGGTCTGATGCTCCACATTCTTCCCGCCTATGTAAGACAGTGGTCCAATGATTTACGTGACCGCTCTACATTATCAGGAATTGAATCGTTCACAAGAGCATGGTGCAGCCCACATCTCATCGCTGGCGAATTAAGTCAGATTAAAAAGGATGAAATTGCTGACGAGAATTTTACCATTGCTGTAAGCAAATCAGCAAATGAGGTTGTTGCTACTTATACAAAGGATGAGACTGCAATGAATCTAGTAATCCGTCTTCCTTCATCTTACCCATTACGGCCTGTTGATGTTGATTGTACAAGGAGTCTAGGTATTAGTGAAGCGAAGCAGAGGAAATGGTCAATGTCTATGACATCATTCGTACGGAATCAGAATGGAGCTTTAGCAGAAGCTATCCGGATATGGAAGCGCAACTTTGATAAGGAGTTTGAAGGTGTCGAGGAGTGTCCTATTTGTTACAGTGTCATCCATACCGTCAACCACGCCCTTCCACGTCTAGCGTGCAAGACCTGCAAGCACAAGTTCCATTCGGCCTGCCTCTATAAGTGGTTCTCAACATCTCACAAATCAACTTGCCCATTGTGCCAGTCTCCATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004019 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006163 GO:0006164 GO:0006167 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010035 GO:0010038 GO:0016874 GO:0016879 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0034641 GO:0034654 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044208 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046033 GO:0046040 GO:0046390 GO:0046483 GO:0046686 GO:0048046 GO:0050896 GO:0055086 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00230 ko00250 ko01100 map00230 map00250 map01100

Pfam Domains

Protein Families

Adenylsucc_synt

Protein Analysis

1903

Amino Acids

212.55

Weight (kDa)

5.64

Isoelectric Point (pI)

47.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ltn1_1st	PF22958	60 - 387	5.7e-88	E3 ubiquitin-protein ligase listerin, N-terminal domain
LTN1_E3_ligase_6th	PF22999	1510 - 1753	4.8e-28	LTN1 family HEAT repeat region
UBC_like	PF23009	1763 - 1846	8.7e-30	Ubiquitin conjugating domain-like
zf-RING_2	PF13639	1852 - 1900	7.6e-07	Ring finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002974)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57610
fragaria_vesca	FvH4_2g02690 FvH4_7g07940
malus_domestica	MD00G1080700.v1.1 MD05G1111200.v1.1 MD15G1386600.v1.1
prunus_persica	Prupe.5G015200_v2.0.a1 Prupe.5G015200_v2.0.a1 Prupe.5G015200_v2.0.a1 Prupe.5G015200_v2.0.a1 Prupe.8G156300_v2.0.a1 Prupe.8G156300_v2.0.a1 Prupe.8G156300_v2.0.a1
pyrus_communis	pycom05g10700 pycom10g09910 pycom15g34650
rosa_chinensis	RchiOBHm_Chr6g0247091 RchiOBHm_Chr6g0247101 RchiOBHm_Chr7g0177111 RchiOBHm_Chr7g0177151
rosa_laevigata	RLG00000015220 RLG00000030365
rosa_multiflora	Rmu_sc0005310.1_g000020 Rmu_sc0010391.1_g000001 Rmu_sc0012524.1_g000001
rosa_roxburghii	Rroxscaffold_3G00276060 Rroxscaffold_7G00214350
rosa_rugosa	Rorug05G0522100 Rorug05G0522200 Rorug05G0522300
rosa_samantha	Rh6AG037300 Rh6BG032200 Rh6CG033300 Rh6DG031000 Rh7AG003500 Rh7CG003200
rosa_wichuraiana	Rw6G003200 Rw7G000290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	899
AarI	CACCTGC	1 cut(s)	649
AasI	GACNNNNNNGTC	1 cut(s)	4239
Acc36I	ACCTGC	3 cut(s)	649, 2462, 5630
Acc65I	GGTACC	1 cut(s)	3494
AccB1I	GGYRCC	2 cut(s)	734, 3494
AccBSI	CCGCTC	1 cut(s)	5178
AccI	GTMKAC	1 cut(s)	4706
AccIII	TCCGGA	2 cut(s)	3888, 5508
AcoI	YGGCCR	4 cut(s)	297, 687, 3067, 3233
AcuI	CTGAAG	1 cut(s)	495
AcvI	CACGTG	1 cut(s)	3744
AcyI	GRCGYC	1 cut(s)	65
AdeI	CACNNNGTG	2 cut(s)	2600, 3747
AfiI	CCNNNNNNNGG	7 cut(s)	146, 311, 402, 1044, 1925, 2306, 4434
AflII	CTTAAG	2 cut(s)	3442, 4964
AflIII	ACRYGT	3 cut(s)	4701, 4807, 5021
AhlI	ACTAGT	1 cut(s)	4882
AjiI	CACGTC	1 cut(s)	5606
AjnI	CCWGG	7 cut(s)	730, 2103, 2723, 3197, 3235, 3306, 4626
AloI	GAACNNNNNNTCC	4 cut(s)	1965, 1966, 1997, 1998
Alw21I	GWGCWC	2 cut(s)	2023, 3451
AlwNI	CAGNNNCTG	3 cut(s)	473, 1844, 5006
Ama87I	CYCGRG	1 cut(s)	4775
Aor13HI	TCCGGA	2 cut(s)	3888, 5508
AseI	ATTAAT	1 cut(s)	3927
Asp700I	GAANNNNTTC	4 cut(s)	1290, 2745, 4983, 5038
Asp718I	GGTACC	1 cut(s)	3494
AspLEI	GCGC	3 cut(s)	418, 2312, 5523
AsuC2I	CCSGG	2 cut(s)	1446, 2491
AsuHPI	GGTGA	7 cut(s)	385, 673, 1806, 1940, 2612, 2750, 2996
AvaI	CYCGRG	1 cut(s)	4775
AvaII	GGWCC	9 cut(s)	440, 1769, 1859, 2965, 4232, 4297, 4526, 4824, 5157
BalI	TGGCCA	3 cut(s)	689, 3069, 3235
BamHI	GGATCC	2 cut(s)	979, 2938
BanI	GGYRCC	2 cut(s)	734, 3494
BanII	GRGCYC	1 cut(s)	3451
BarI	GAAGNNNNNNTAC	2 cut(s)	5352, 5384
BauI	CACGAG	2 cut(s)	3223, 4385
BbrPI	CACGTG	1 cut(s)	3744
BbsI	GAAGAC	2 cut(s)	4650, 5360
Bbv12I	GWGCWC	2 cut(s)	2023, 3451
BbvCI	CCTCAGC	1 cut(s)	4359
BceAI	ACGGC	3 cut(s)	61, 573, 5405
BcgI	CGANNNNNNTGC	2 cut(s)	1721, 1755
BciT130I	CCWGG	7 cut(s)	732, 2105, 2725, 3199, 3237, 3308, 4628
BclI	TGATCA	1 cut(s)	3738
BcnI	CCSGG	2 cut(s)	1446, 2491
BcuI	ACTAGT	1 cut(s)	4882
BfmI	CTRYAG	6 cut(s)	1614, 3249, 4049, 4185, 5007, 5013
BfoI	RGCGCY	2 cut(s)	419, 2313
BfrI	CTTAAG	2 cut(s)	3442, 4964
BfuAI	ACCTGC	3 cut(s)	649, 2462, 5630
BlpI	GCTNAGC	1 cut(s)	4517
BmcAI	AGTACT	1 cut(s)	2565
Bme1390I	CCNGG	9 cut(s)	732, 1446, 2105, 2491, 2725, 3199, 3237, 3308, 4628
Bme18I	GGWCC	9 cut(s)	440, 1769, 1859, 2965, 4232, 4297, 4526, 4824, 5157
BmeT110I	CYCGRG	1 cut(s)	4775
BmgBI	CACGTC	1 cut(s)	5606
BmiI	GGNNCC	9 cut(s)	441, 736, 981, 2940, 3496, 4298, 4299, 4528, 5041
BmrFI	CCNGG	9 cut(s)	732, 1446, 2105, 2491, 2725, 3199, 3237, 3308, 4628
BmrI	ACTGGG	2 cut(s)	461, 2662
BmuI	ACTGGG	2 cut(s)	461, 2662
BoxI	GACNNNNGTC	2 cut(s)	1052, 5155
BpiI	GAAGAC	2 cut(s)	4650, 5360
BpmI	CTGGAG	5 cut(s)	507, 1607, 2126, 3860, 5124
Bpu10I	CCTNAGC	1 cut(s)	4359
Bpu1102I	GCTNAGC	1 cut(s)	4517
BpuEI	CTTGAG	4 cut(s)	468, 897, 1300, 1405
BpuMI	CCSGG	2 cut(s)	1446, 2491
BsaAI	YACGTR	4 cut(s)	1518, 3744, 4894, 5171
BsaBI	GATNNNNATC	1 cut(s)	2153
BsaHI	GRCGYC	1 cut(s)	65
BsaJI	CCNNGG	8 cut(s)	443, 1444, 1985, 2490, 2980, 3042, 3306, 4150
BsaWI	WCCGGW	3 cut(s)	2145, 3888, 5508
BsaXI	ACNNNNNCTCC	6 cut(s)	3142, 3172, 4010, 4040, 5407, 5437
Bsc4I	CCNNNNNNNGG	7 cut(s)	146, 311, 402, 1044, 1925, 2306, 4434
Bse1I	ACTGG	7 cut(s)	467, 2657, 3938, 4888, 4913, 5041, 5698
Bse8I	GATNNNNATC	1 cut(s)	2153
BseAI	TCCGGA	2 cut(s)	3888, 5508
BseBI	CCWGG	7 cut(s)	732, 2105, 2725, 3199, 3237, 3308, 4628
BseDI	CCNNGG	8 cut(s)	443, 1444, 1985, 2490, 2980, 3042, 3306, 4150
BseJI	GATNNNNATC	1 cut(s)	2153
BseLI	CCNNNNNNNGG	7 cut(s)	146, 311, 402, 1044, 1925, 2306, 4434
BseMII	CTCAG	8 cut(s)	581, 1617, 2058, 2806, 2948, 4046, 4350, 4508
BseNI	ACTGG	7 cut(s)	467, 2657, 3938, 4888, 4913, 5041, 5698
BseRI	GAGGAG	5 cut(s)	129, 1445, 2289, 2292, 5567
BseYI	CCCAGC	1 cut(s)	2728
BsgI	GTGCAG	6 cut(s)	936, 2106, 3831, 3965, 4330, 5239
Bsh1285I	CGRYCG	1 cut(s)	4243
BshNI	GGYRCC	2 cut(s)	734, 3494
BsiEI	CGRYCG	1 cut(s)	4243
BsiHKAI	GWGCWC	2 cut(s)	2023, 3451
BsiHKCI	CYCGRG	1 cut(s)	4775
BsiSI	CCGG	6 cut(s)	147, 1446, 2146, 2490, 3889, 5509
BsiWI	CGTACG	1 cut(s)	5473
BslFI	GGGAC	3 cut(s)	453, 893, 4310
BslI	CCNNNNNNNGG	7 cut(s)	146, 311, 402, 1044, 1925, 2306, 4434
BsmBI	CGTCTC	3 cut(s)	12, 1905, 3645
BsmFI	GGGAC	3 cut(s)	453, 893, 4310
BsmI	GAATGC	3 cut(s)	1240, 1292, 2110
BsoBI	CYCGRG	1 cut(s)	4775
Bsp1286I	GDGCHC	2 cut(s)	2023, 3451
Bsp13I	TCCGGA	2 cut(s)	3888, 5508
Bsp1407I	TGTACA	3 cut(s)	1060, 5056, 5408
Bsp1720I	GCTNAGC	1 cut(s)	4517
Bsp19I	CCATGG	2 cut(s)	443, 1985
BspCNI	CTCAG	8 cut(s)	582, 1616, 2059, 2805, 2947, 4045, 4351, 4509
BspEI	TCCGGA	2 cut(s)	3888, 5508
BspHI	TCATGA	2 cut(s)	370, 2254
BspLI	GGNNCC	9 cut(s)	441, 736, 981, 2940, 3496, 4298, 4299, 4528, 5041
BspMAI	CTGCAG	4 cut(s)	1618, 4053, 5011, 5017
BspMI	ACCTGC	3 cut(s)	649, 2462, 5630
BspQI	GCTCTTC	1 cut(s)	4974
BspT107I	GGYRCC	2 cut(s)	734, 3494
BspTI	CTTAAG	2 cut(s)	3442, 4964
BsrBI	CCGCTC	1 cut(s)	5178
BsrGI	TGTACA	3 cut(s)	1060, 5056, 5408
BsrI	ACTGG	7 cut(s)	467, 2657, 3938, 4888, 4913, 5041, 5698
BssECI	CCNNGG	8 cut(s)	443, 1444, 1985, 2490, 2980, 3042, 3306, 4150
BssNI	GRCGYC	1 cut(s)	65
BssSI	CACGAG	2 cut(s)	3223, 4385
BssT1I	CCWWGG	5 cut(s)	443, 1985, 2980, 3042, 4150
Bst2BI	CACGAG	2 cut(s)	3223, 4385
Bst2UI	CCWGG	7 cut(s)	732, 2105, 2725, 3199, 3237, 3308, 4628
Bst6I	CTCTTC	8 cut(s)	197, 2403, 2856, 3281, 3307, 4674, 4937, 4974
BstACI	GRCGYC	1 cut(s)	65
BstAFI	CTTAAG	2 cut(s)	3442, 4964
BstAPI	GCANNNNNTGC	8 cut(s)	649, 1181, 1597, 3560, 3952, 4259, 5006, 5622
BstAUI	TGTACA	3 cut(s)	1060, 5056, 5408
BstBAI	YACGTR	4 cut(s)	1518, 3744, 4894, 5171
BstDSI	CCRYGG	2 cut(s)	443, 1985
BstH2I	RGCGCY	2 cut(s)	419, 2313
BstHHI	GCGC	3 cut(s)	418, 2312, 5523
BstMCI	CGRYCG	1 cut(s)	4243
BstNI	CCWGG	7 cut(s)	732, 2105, 2725, 3199, 3237, 3308, 4628
BstNSI	RCATGY	5 cut(s)	767, 1655, 1727, 4705, 4811
BstPAI	GACNNNNGTC	2 cut(s)	1052, 5155
BstSCI	CCNGG	9 cut(s)	730, 1444, 2103, 2489, 2723, 3197, 3235, 3306, 4626
BstSFI	CTRYAG	6 cut(s)	1614, 3249, 4049, 4185, 5007, 5013
BstSNI	TACGTA	1 cut(s)	4894
BstV2I	GAAGAC	2 cut(s)	4650, 5360
BstX2I	RGATCY	7 cut(s)	220, 979, 1115, 2117, 2843, 2938, 4174
BstXI	CCANNNNNNTGG	3 cut(s)	2104, 2247, 3065
BstYI	RGATCY	7 cut(s)	220, 979, 1115, 2117, 2843, 2938, 4174
BtgI	CCRYGG	2 cut(s)	443, 1985
BtgZI	GCGATG	4 cut(s)	165, 1221, 4848, 5218
BtrI	CACGTC	1 cut(s)	5606
BtsI	GCAGTG	5 cut(s)	599, 813, 1845, 2078, 4497
BveI	ACCTGC	3 cut(s)	649, 2462, 5630
CaiI	CAGNNNCTG	3 cut(s)	473, 1844, 5006
CciI	TCATGA	2 cut(s)	370, 2254
CfoI	GCGC	3 cut(s)	418, 2312, 5523
CseI	GACGC	3 cut(s)	54, 3662, 4050
CspCI	CAANNNNNGTGG	2 cut(s)	679, 714
DraIII	CACNNNGTG	2 cut(s)	2600, 3747
DrdI	GACNNNNNNGTC	1 cut(s)	4239
DseDI	GACNNNNNNGTC	1 cut(s)	4239
EaeI	YGGCCR	4 cut(s)	297, 687, 3067, 3233
Eam1104I	CTCTTC	8 cut(s)	197, 2403, 2856, 3281, 3307, 4674, 4937, 4974
EarI	CTCTTC	8 cut(s)	197, 2403, 2856, 3281, 3307, 4674, 4937, 4974
EciI	GGCGGA	2 cut(s)	2836, 3720
Ecl136II	GAGCTC	1 cut(s)	3449
Eco105I	TACGTA	1 cut(s)	4894
Eco130I	CCWWGG	5 cut(s)	443, 1985, 2980, 3042, 4150
Eco24I	GRGCYC	1 cut(s)	3451
Eco32I	GATATC	2 cut(s)	666, 3105
Eco47I	GGWCC	9 cut(s)	440, 1769, 1859, 2965, 4232, 4297, 4526, 4824, 5157
Eco53kI	GAGCTC	1 cut(s)	3449
Eco57I	CTGAAG	1 cut(s)	495
Eco72I	CACGTG	1 cut(s)	3744
Eco88I	CYCGRG	1 cut(s)	4775
EcoICRI	GAGCTC	1 cut(s)	3449
EcoO109I	RGGNCCY	3 cut(s)	162, 4297, 4526
EcoRI	GAATTC	2 cut(s)	1202, 4505
EcoRII	CCWGG	7 cut(s)	730, 2103, 2723, 3197, 3235, 3306, 4626
EcoRV	GATATC	2 cut(s)	666, 3105
EcoT14I	CCWWGG	5 cut(s)	443, 1985, 2980, 3042, 4150
EcoT22I	ATGCAT	3 cut(s)	654, 2692, 2755
EcoT38I	GRGCYC	1 cut(s)	3451
ErhI	CCWWGG	5 cut(s)	443, 1985, 2980, 3042, 4150
Esp3I	CGTCTC	3 cut(s)	12, 1905, 3645
FalI	AAGNNNNNCTT	6 cut(s)	1033, 1065, 4314, 4346, 5510, 5542
FaqI	GGGAC	3 cut(s)	453, 893, 4310
FauI	CCCGC	2 cut(s)	47, 5146
FbaI	TGATCA	1 cut(s)	3738
FblI	GTMKAC	1 cut(s)	4706
FriOI	GRGCYC	1 cut(s)	3451
GlaI	GCGC	3 cut(s)	417, 2311, 5522
GsaI	CCCAGC	1 cut(s)	2732
GsuI	CTGGAG	5 cut(s)	507, 1607, 2126, 3860, 5124
HaeII	RGCGCY	2 cut(s)	419, 2313
HapII	CCGG	6 cut(s)	147, 1446, 2146, 2490, 3889, 5509
HgaI	GACGC	3 cut(s)	54, 3662, 4050
HhaI	GCGC	3 cut(s)	418, 2312, 5523
Hin1I	GRCGYC	1 cut(s)	65
Hin6I	GCGC	3 cut(s)	416, 2310, 5521
HinP1I	GCGC	3 cut(s)	416, 2310, 5521
HincII	GTYRAC	3 cut(s)	4462, 4707, 5590
HindII	GTYRAC	3 cut(s)	4462, 4707, 5590
HindIII	AAGCTT	4 cut(s)	233, 240, 1385, 3962
HpaII	CCGG	6 cut(s)	147, 1446, 2146, 2490, 3889, 5509
HphI	GGTGA	7 cut(s)	385, 673, 1806, 1940, 2612, 2750, 2996
Hpy99I	CGWCG	3 cut(s)	70, 82, 119
HpyCH4IV	ACGT	9 cut(s)	114, 906, 1517, 1898, 3743, 4893, 5023, 5170, 5605
HpySE526I	ACGT	9 cut(s)	114, 906, 1517, 1898, 3743, 4893, 5023, 5170, 5605
Hsp92I	GRCGYC	1 cut(s)	65
HspAI	GCGC	3 cut(s)	416, 2310, 5521
KflI	GGGWCCC	1 cut(s)	4297
Kpn2I	TCCGGA	2 cut(s)	3888, 5508
KpnI	GGTACC	1 cut(s)	3498
Ksp22I	TGATCA	1 cut(s)	3738
LguI	GCTCTTC	1 cut(s)	4974
MaeII	ACGT	9 cut(s)	114, 906, 1517, 1898, 3743, 4893, 5023, 5170, 5605
MbiI	CCGCTC	1 cut(s)	5178
MflI	RGATCY	7 cut(s)	220, 979, 1115, 2117, 2843, 2938, 4174
MhlI	GDGCHC	2 cut(s)	2023, 3451
MlsI	TGGCCA	3 cut(s)	689, 3069, 3235
MluNI	TGGCCA	3 cut(s)	689, 3069, 3235
MlyI	GAGTC	3 cut(s)	3184, 3635, 5425
MmeI	TCCRAC	6 cut(s)	97, 1025, 1509, 1747, 3526, 3971
Mox20I	TGGCCA	3 cut(s)	689, 3069, 3235
Mph1103I	ATGCAT	3 cut(s)	654, 2692, 2755
MroI	TCCGGA	2 cut(s)	3888, 5508
MroXI	GAANNNNTTC	4 cut(s)	1290, 2745, 4983, 5038
MscI	TGGCCA	3 cut(s)	689, 3069, 3235
MslI	CAYNNNNRTG	4 cut(s)	1000, 2228, 2758, 2893
Msp20I	TGGCCA	3 cut(s)	689, 3069, 3235
MspA1I	CMGCKG	4 cut(s)	424, 3784, 3920, 5012
MspCI	CTTAAG	2 cut(s)	3442, 4964
MspI	CCGG	6 cut(s)	147, 1446, 2146, 2490, 3889, 5509
MspR9I	CCNGG	9 cut(s)	732, 1446, 2105, 2491, 2725, 3199, 3237, 3308, 4628
Mva1269I	GAATGC	3 cut(s)	1240, 1292, 2110
MvaI	CCWGG	7 cut(s)	732, 2105, 2725, 3199, 3237, 3308, 4628
NciI	CCSGG	2 cut(s)	1446, 2491
NcoI	CCATGG	2 cut(s)	443, 1985
NlaIV	GGNNCC	9 cut(s)	441, 736, 981, 2940, 3496, 4298, 4299, 4528, 5041
NmeAIII	GCCGAG	1 cut(s)	2286
NmuCI	GTSAC	3 cut(s)	391, 4382, 5171
NsiI	ATGCAT	3 cut(s)	654, 2692, 2755
NspI	RCATGY	5 cut(s)	767, 1655, 1727, 4705, 4811
PaeI	GCATGC	1 cut(s)	1655
PaeR7I	CTCGAG	1 cut(s)	4775
PagI	TCATGA	2 cut(s)	370, 2254
PaqCI	CACCTGC	1 cut(s)	649
PciI	ACATGT	2 cut(s)	4701, 4807
PciSI	GCTCTTC	1 cut(s)	4974
PcsI	WCGNNNNNNNCGW	2 cut(s)	74, 111
PctI	GAATGC	3 cut(s)	1240, 1292, 2110
PdmI	GAANNNNTTC	4 cut(s)	1290, 2745, 4983, 5038
Pfl23II	CGTACG	1 cut(s)	5473
PflFI	GACNNNGTC	1 cut(s)	467
PleI	GAGTC	3 cut(s)	3184, 3635, 5424
PmaCI	CACGTG	1 cut(s)	3744
PmlI	CACGTG	1 cut(s)	3744
PpsI	GAGTC	3 cut(s)	3184, 3635, 5424
Ppu21I	YACGTR	4 cut(s)	1518, 3744, 4894, 5171
PpuMI	RGGWCCY	2 cut(s)	4297, 4526
PscI	ACATGT	2 cut(s)	4701, 4807
PshAI	GACNNNNGTC	2 cut(s)	1052, 5155
PshBI	ATTAAT	1 cut(s)	3927
PsiI	TTATAA	1 cut(s)	899
Psp124BI	GAGCTC	1 cut(s)	3451
Psp5II	RGGWCCY	2 cut(s)	4297, 4526
Psp6I	CCWGG	7 cut(s)	730, 2103, 2723, 3197, 3235, 3306, 4626
PspCI	CACGTG	1 cut(s)	3744
PspFI	CCCAGC	1 cut(s)	2728
PspGI	CCWGG	7 cut(s)	730, 2103, 2723, 3197, 3235, 3306, 4626
PspLI	CGTACG	1 cut(s)	5473
PspN4I	GGNNCC	9 cut(s)	441, 736, 981, 2940, 3496, 4298, 4299, 4528, 5041
PspPPI	RGGWCCY	2 cut(s)	4297, 4526
PsrI	GAACNNNNNNTAC	2 cut(s)	4605, 4637
PstI	CTGCAG	4 cut(s)	1618, 4053, 5011, 5017
PstNI	CAGNNNCTG	3 cut(s)	473, 1844, 5006
PsuI	RGATCY	7 cut(s)	220, 979, 1115, 2117, 2843, 2938, 4174
PsyI	GACNNNGTC	1 cut(s)	467
PvuII	CAGCTG	4 cut(s)	424, 3784, 3920, 5012
RseI	CAYNNNNRTG	4 cut(s)	1000, 2228, 2758, 2893
SacI	GAGCTC	1 cut(s)	3451
SalI	GTCGAC	1 cut(s)	4705
SapI	GCTCTTC	1 cut(s)	4974
ScaI	AGTACT	1 cut(s)	2565
SchI	GAGTC	3 cut(s)	3184, 3635, 5425
ScrFI	CCNGG	9 cut(s)	732, 1446, 2105, 2491, 2725, 3199, 3237, 3308, 4628
SduI	GDGCHC	2 cut(s)	2023, 3451
SfcI	CTRYAG	6 cut(s)	1614, 3249, 4049, 4185, 5007, 5013
Sfr274I	CTCGAG	1 cut(s)	4775
SinI	GGWCC	9 cut(s)	440, 1769, 1859, 2965, 4232, 4297, 4526, 4824, 5157
SlaI	CTCGAG	1 cut(s)	4775
SmiMI	CAYNNNNRTG	4 cut(s)	1000, 2228, 2758, 2893
SmlI	CTYRAG	7 cut(s)	483, 912, 1315, 1420, 3442, 4775, 4964
SmoI	CTYRAG	7 cut(s)	483, 912, 1315, 1420, 3442, 4775, 4964
SnaBI	TACGTA	1 cut(s)	4894
SpeI	ACTAGT	1 cut(s)	4882
SphI	GCATGC	1 cut(s)	1655
SspI	AATATT	4 cut(s)	827, 2060, 3216, 4920
SstI	GAGCTC	1 cut(s)	3451
StyD4I	CCNGG	9 cut(s)	730, 1444, 2103, 2489, 2723, 3197, 3235, 3306, 4626
StyI	CCWWGG	5 cut(s)	443, 1985, 2980, 3042, 4150
TaiI	ACGT	9 cut(s)	117, 909, 1520, 1901, 3746, 4896, 5026, 5173, 5608
TaqII	GACCGA	1 cut(s)	4229
TatI	WGTACW	6 cut(s)	1060, 1072, 2333, 2563, 5056, 5408
TauI	GCSGC	5 cut(s)	65, 95, 299, 361, 2577
TseFI	GTSAC	3 cut(s)	391, 4382, 5171
Tsp45I	GTSAC	3 cut(s)	391, 4382, 5171
TspGWI	ACGGA	5 cut(s)	36, 854, 2202, 5354, 5491
Tth111I	GACNNNGTC	1 cut(s)	467
Vha464I	CTTAAG	2 cut(s)	3442, 4964
VpaK11BI	GGWCC	9 cut(s)	440, 1769, 1859, 2965, 4232, 4297, 4526, 4824, 5157
VspI	ATTAAT	1 cut(s)	3927
XbaI	TCTAGA	2 cut(s)	2263, 4948
XceI	RCATGY	5 cut(s)	767, 1655, 1727, 4705, 4811
XhoI	CTCGAG	1 cut(s)	4775
XmiI	GTMKAC	1 cut(s)	4706
XmnI	GAANNNNTTC	4 cut(s)	1290, 2745, 4983, 5038
ZrmI	AGTACT	1 cut(s)	2565
Zsp2I	ATGCAT	3 cut(s)	654, 2692, 2755

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.