Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002134

Physical Location & Seq

Genomic Coordinates Reverse (-)

33533127 .. 33537089

Gene Structure 3963 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1206200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 903 bp

ATGGCCAACAACCCCTCGGAGCCCCAGGCCGACGACTTCCTCGAGCAAATTCTCGGCCTCCCCAACTTTGCCTCCGCCGATGCTAATTTGGCGGGAAGCGACGGCGGTTTGGCCGGAGGACAGGTATCTCCGGCGTCTATGATGCTGCAGCTGAACTCCGTCGACGGGTCCGGCCACCTAGGCGGAGTTGGCGGTGGCGGTGGGTTCCATGGAGGGGTGTTTCCCTTGGGGTTGAGCTTGGAGCAGGGGAAAGCTGGGTTTTTGAAGCACGAGGAGGCTTCTGGGAGCGGGAAGCGGTTTCGAGACGACGTCGTTGATAGTCGAGTTTCATCTGTGAAAAATGTTTTCCATGGCCAACCTATTTCAAATACAGTTGCTGCAGCACCGCATCCACCAGCAATGCGACCAAGGGTGCGAGCTAGAAGAGGACAAGCCACAGATCCACACAGCATTGCCGAGCGGTTGCGCAGAGAAAGAATAGCAGAAAGAATCAGGGCGTTGCAGGAACTAGTTCCTAGCGTCAACAAGACGGATAGAGCTGCCATGCTTGATGAGATAATGGACTATGTGAAGTTCCTAAGGCTCCAAGTAAAGGTTTTGAGCATGAGTAGATTGGGCGGGGCTGGTGCTGTGGCACCACTTGTAACAGATATTCCACTATCTTCAGTTGAGGAAGAAGGCAGCGAAGGTGGTAGAAACCAACCGGCGTGGGATAAGTGGTCTAATGACGGCACAGAACGACAAGTAGCTAAGCTTATGGAAGACAACGTTGGGGCGGCAATGCAGTTCCTCCAATACAAGGCTCTCTGCATCATGCCTATCTCACTGGCCTCGGCAATCTACAACACACAACCTCCGGACACCTCTAGCGTTGTCAAGCCTGAAATGAACCCTCCTTCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000976 GO:0000981 GO:0001046 GO:0001047 GO:0001067 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009566 GO:0009567 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019953 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0044703 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

301

Amino Acids

31.86

Weight (kDa)

5.9

Isoelectric Point (pI)

45.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	149 - 194	1.9e-07	Helix-loop-helix DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002134)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02590 AT4G02590 AT4G02590
fragaria_vesca	FvH4_2g22560 FvH4_2g22560 FvH4_6g02900 FvH4_6g02900 FvH4_6g02900 FvH4_6g02900 FvH4_6g02900 FvH4_6g02900
malus_domestica	MD04G1224700.v1.1 MD05G1206200.v1.1 MD10G1193200.v1.1 MD12G1241500.v1.1
prunus_persica	Prupe.6G343400_v2.0.a1 Prupe.6G343400_v2.0.a1 Prupe.8G232600_v2.0.a1
pyrus_communis	pycom04g19790 pycom10g16690 pycom12g22100
rosa_chinensis	RchiOBHm_Chr3g0451111 RchiOBHm_Chr6g0289601
rosa_laevigata	RLG00000012289 RLG00000025689
rosa_multiflora	Rmu_sc0005376.1_g000001
rosa_roxburghii	Rroxscaffold_6G00428580 Rroxscaffold_7G00177820
rosa_rugosa	Rorug02G0631400 Rorug02G0631500 Rorug02G0631600 Rorug02G0631700 Rorug02G0631800 Rorug06G0205100
rosa_samantha	Rh3AG034400 Rh3BG034700 Rh3CG033400 Rh3DG034400 Rh6AG316600 Rh6BG323000 Rh6CG329600 Rh6DG315000
rosa_wichuraiana	Rw3G002500 Rw6G027300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	312
Acc16I	TGCGCA	1 cut(s)	467
AccB1I	GGYRCC	1 cut(s)	634
AccBSI	CCGCTC	2 cut(s)	288, 460
AccI	GTMKAC	1 cut(s)	162
AccIII	TCCGGA	1 cut(s)	856
AclI	AACGTT	1 cut(s)	768
AclWI	GGATC	1 cut(s)	434
AcoI	YGGCCR	4 cut(s)	3, 111, 172, 352
AcsI	RAATTY	1 cut(s)	48
AcuI	CTGAAG	1 cut(s)	648
AcyI	GRCGYC	2 cut(s)	134, 309
AfiI	CCNNNNNNNGG	4 cut(s)	165, 214, 592, 799
AgsI	TTSAA	2 cut(s)	265, 366
AhlI	ACTAGT	1 cut(s)	508
AjnI	CCWGG	1 cut(s)	24
AjuI	GAANNNNNNNTTGG	2 cut(s)	753, 785
AloI	GAACNNNNNNTCC	1 cut(s)	881
AluBI	AGCT	7 cut(s)	151, 237, 254, 419, 539, 749, 754
AluI	AGCT	7 cut(s)	151, 237, 254, 419, 539, 749, 754
Alw26I	GTCTC	1 cut(s)	297
AlwI	GGATC	1 cut(s)	434
AlwNI	CAGNNNCTG	1 cut(s)	377
Ama87I	CYCGRG	1 cut(s)	41
Aor13HI	TCCGGA	1 cut(s)	856
AoxI	GGCC	7 cut(s)	3, 27, 55, 111, 172, 352, 828
ApeKI	GCWGC	6 cut(s)	145, 148, 377, 380, 539, 681
ApoI	RAATTY	1 cut(s)	48
Asp700I	GAANNNNTTC	1 cut(s)	510
AspA2I	CCTAGG	1 cut(s)	178
AspLEI	GCGC	1 cut(s)	468
AspS9I	GGNCC	1 cut(s)	168
AvaI	CYCGRG	1 cut(s)	41
AvaII	GGWCC	1 cut(s)	168
AvrII	CCTAGG	1 cut(s)	178
AxyI	CCTNAGG	1 cut(s)	578
BalI	TGGCCA	2 cut(s)	5, 354
BanI	GGYRCC	1 cut(s)	634
BanII	GRGCYC	1 cut(s)	24
BauI	CACGAG	1 cut(s)	269
BbsI	GAAGAC	1 cut(s)	768
BbvI	GCAGC	6 cut(s)	132, 160, 364, 392, 526, 693
BceAI	ACGGC	2 cut(s)	118, 745
BciT130I	CCWGG	1 cut(s)	26
BcoDI	GTCTC	1 cut(s)	297
BcuI	ACTAGT	1 cut(s)	508
BfaI	CTAG	5 cut(s)	179, 420, 509, 516, 867
BfmI	CTRYAG	2 cut(s)	146, 378
BglI	GCCNNNNNGGC	1 cut(s)	180
BisI	GCNGC	7 cut(s)	146, 149, 378, 381, 540, 682, 777
BlnI	CCTAGG	1 cut(s)	178
BlpI	GCTNAGC	1 cut(s)	750
BlsI	GCNGC	7 cut(s)	147, 150, 379, 382, 541, 683, 778
Bme1390I	CCNGG	1 cut(s)	26
Bme18I	GGWCC	1 cut(s)	168
BmeT110I	CYCGRG	1 cut(s)	41
BmgT120I	GGNCC	1 cut(s)	168
BmiI	GGNNCC	5 cut(s)	21, 169, 206, 584, 636
BmrFI	CCNGG	1 cut(s)	26
BmsI	GCATC	4 cut(s)	70, 132, 397, 819
BpiI	GAAGAC	1 cut(s)	768
Bpu1102I	GCTNAGC	1 cut(s)	750
BsaHI	GRCGYC	2 cut(s)	134, 309
BsaJI	CCNNGG	8 cut(s)	15, 24, 178, 208, 225, 349, 407, 831
BsaWI	WCCGGW	1 cut(s)	856
BsaXI	ACNNNNNCTCC	6 cut(s)	56, 86, 108, 138, 838, 868
Bsc4I	CCNNNNNNNGG	4 cut(s)	165, 214, 592, 799
Bse118I	RCCGGY	1 cut(s)	703
Bse1I	ACTGG	1 cut(s)	831
Bse21I	CCTNAGG	1 cut(s)	578
Bse3DI	GCAATG	3 cut(s)	405, 450, 786
BseAI	TCCGGA	1 cut(s)	856
BseBI	CCWGG	1 cut(s)	26
BseDI	CCNNGG	8 cut(s)	15, 24, 178, 208, 225, 349, 407, 831
BseGI	GGATG	1 cut(s)	388
BseLI	CCNNNNNNNGG	4 cut(s)	165, 214, 592, 799
BseMI	GCAATG	3 cut(s)	405, 450, 786
BseNI	ACTGG	1 cut(s)	831
BseRI	GAGGAG	1 cut(s)	287
BseXI	GCAGC	6 cut(s)	132, 160, 364, 392, 526, 693
BseYI	CCCAGC	1 cut(s)	254
BshFI	GGCC	7 cut(s)	5, 29, 57, 113, 174, 354, 830
BshNI	GGYRCC	1 cut(s)	634
BsiHKCI	CYCGRG	1 cut(s)	41
BsiSI	CCGG	5 cut(s)	114, 131, 171, 704, 857
BslI	CCNNNNNNNGG	4 cut(s)	165, 214, 592, 799
BsmAI	GTCTC	1 cut(s)	297
BsmBI	CGTCTC	1 cut(s)	297
BsnI	GGCC	7 cut(s)	5, 29, 57, 113, 174, 354, 830
BsoBI	CYCGRG	1 cut(s)	41
Bsp1286I	GDGCHC	1 cut(s)	24
Bsp13I	TCCGGA	1 cut(s)	856
Bsp143I	GATC	1 cut(s)	439
Bsp1720I	GCTNAGC	1 cut(s)	750
Bsp19I	CCATGG	2 cut(s)	208, 349
BspANI	GGCC	7 cut(s)	5, 29, 57, 113, 174, 354, 830
BspEI	TCCGGA	1 cut(s)	856
BspLI	GGNNCC	5 cut(s)	21, 169, 206, 584, 636
BspMAI	CTGCAG	2 cut(s)	150, 382
BspPI	GGATC	1 cut(s)	434
BspT107I	GGYRCC	1 cut(s)	634
BsrBI	CCGCTC	2 cut(s)	288, 460
BsrDI	GCAATG	3 cut(s)	405, 450, 786
BsrFI	RCCGGY	1 cut(s)	703
BsrI	ACTGG	1 cut(s)	831
BssAI	RCCGGY	1 cut(s)	703
BssECI	CCNNGG	8 cut(s)	15, 24, 178, 208, 225, 349, 407, 831
BssMI	GATC	1 cut(s)	439
BssNI	GRCGYC	2 cut(s)	134, 309
BssSI	CACGAG	1 cut(s)	269
BssT1I	CCWWGG	5 cut(s)	178, 208, 225, 349, 407
Bst2BI	CACGAG	1 cut(s)	269
Bst2UI	CCWGG	1 cut(s)	26
Bst4CI	ACNGT	1 cut(s)	373
Bst6I	CTCTTC	1 cut(s)	418
BstACI	GRCGYC	2 cut(s)	134, 309
BstC8I	GCNNGC	1 cut(s)	417
BstDEI	CTNAG	2 cut(s)	578, 750
BstDSI	CCRYGG	2 cut(s)	208, 349
BstF5I	GGATG	1 cut(s)	388
BstHHI	GCGC	1 cut(s)	468
BstKTI	GATC	1 cut(s)	442
BstMAI	GTCTC	1 cut(s)	297
BstMBI	GATC	1 cut(s)	439
BstMWI	GCNNNNNNNGC	3 cut(s)	89, 180, 189
BstNI	CCWGG	1 cut(s)	26
BstSCI	CCNGG	1 cut(s)	24
BstSFI	CTRYAG	2 cut(s)	146, 378
BstV1I	GCAGC	6 cut(s)	132, 160, 364, 392, 526, 693
BstV2I	GAAGAC	1 cut(s)	768
BstX2I	RGATCY	1 cut(s)	439
BstYI	RGATCY	1 cut(s)	439
Bsu36I	CCTNAGG	1 cut(s)	578
BsuRI	GGCC	7 cut(s)	5, 29, 57, 113, 174, 354, 830
BtgI	CCRYGG	2 cut(s)	208, 349
BtsCI	GGATG	1 cut(s)	388
BtsIMutI	CAGTG	1 cut(s)	824
Cac8I	GCNNGC	1 cut(s)	417
CaiI	CAGNNNCTG	1 cut(s)	377
CfoI	GCGC	1 cut(s)	468
Cfr10I	RCCGGY	1 cut(s)	703
Cfr13I	GGNCC	1 cut(s)	168
CseI	GACGC	2 cut(s)	123, 508
CspCI	CAANNNNNGTGG	2 cut(s)	689, 724
CviAII	CATG	5 cut(s)	209, 350, 544, 604, 814
DdeI	CTNAG	2 cut(s)	578, 750
DpnI	GATC	1 cut(s)	441
DpnII	GATC	1 cut(s)	439
EaeI	YGGCCR	4 cut(s)	3, 111, 172, 352
Eam1104I	CTCTTC	1 cut(s)	418
EarI	CTCTTC	1 cut(s)	418
EciI	GGCGGA	2 cut(s)	64, 198
Eco130I	CCWWGG	5 cut(s)	178, 208, 225, 349, 407
Eco24I	GRGCYC	1 cut(s)	24
Eco47I	GGWCC	1 cut(s)	168
Eco57I	CTGAAG	1 cut(s)	648
Eco81I	CCTNAGG	1 cut(s)	578
Eco88I	CYCGRG	1 cut(s)	41
EcoRII	CCWGG	1 cut(s)	24
EcoT14I	CCWWGG	5 cut(s)	178, 208, 225, 349, 407
EcoT38I	GRGCYC	1 cut(s)	24
ErhI	CCWWGG	5 cut(s)	178, 208, 225, 349, 407
Esp3I	CGTCTC	1 cut(s)	297
FaeI	CATG	5 cut(s)	212, 353, 547, 607, 817
FaiI	YATR	8 cut(s)	140, 210, 351, 545, 567, 605, 758, 815
FatI	CATG	5 cut(s)	208, 349, 543, 603, 813
FauI	CCCGC	3 cut(s)	85, 281, 611
FblI	GTMKAC	1 cut(s)	162
Fnu4HI	GCNGC	7 cut(s)	146, 149, 378, 381, 540, 682, 777
FokI	GGATG	1 cut(s)	375
FriOI	GRGCYC	1 cut(s)	24
Fsp4HI	GCNGC	7 cut(s)	146, 149, 378, 381, 540, 682, 777
FspBI	CTAG	5 cut(s)	179, 420, 509, 516, 867
FspI	TGCGCA	1 cut(s)	467
GlaI	GCGC	1 cut(s)	467
GluI	GCNGC	7 cut(s)	146, 149, 378, 381, 540, 682, 777
GsaI	CCCAGC	1 cut(s)	258
HaeIII	GGCC	7 cut(s)	5, 29, 57, 113, 174, 354, 830
HapII	CCGG	5 cut(s)	114, 131, 171, 704, 857
HgaI	GACGC	2 cut(s)	123, 508
HhaI	GCGC	1 cut(s)	468
Hin1I	GRCGYC	2 cut(s)	134, 309
Hin1II	CATG	5 cut(s)	212, 353, 547, 607, 817
Hin6I	GCGC	1 cut(s)	466
HinP1I	GCGC	1 cut(s)	466
HincII	GTYRAC	2 cut(s)	163, 523
HindII	GTYRAC	2 cut(s)	163, 523
HindIII	AAGCTT	1 cut(s)	752
HinfI	GANTC	1 cut(s)	489
HpaII	CCGG	5 cut(s)	114, 131, 171, 704, 857
Hpy166II	GTNNAC	2 cut(s)	163, 523
Hpy188I	TCNGA	1 cut(s)	19
Hpy188III	TCNNGA	2 cut(s)	302, 857
Hpy8I	GTNNAC	2 cut(s)	163, 523
Hpy99I	CGWCG	6 cut(s)	35, 104, 164, 167, 311, 314
HpyAV	CCTTC	2 cut(s)	671, 680
HpyCH4III	ACNGT	1 cut(s)	373
HpyCH4IV	ACGT	2 cut(s)	309, 768
HpyCH4V	TGCA	5 cut(s)	148, 380, 502, 784, 810
HpyF10VI	GCNNNNNNNGC	3 cut(s)	89, 180, 189
HpyF3I	CTNAG	2 cut(s)	578, 750
HpySE526I	ACGT	2 cut(s)	309, 768
Hsp92I	GRCGYC	2 cut(s)	134, 309
Hsp92II	CATG	5 cut(s)	212, 353, 547, 607, 817
HspAI	GCGC	1 cut(s)	466
Kpn2I	TCCGGA	1 cut(s)	856
Kzo9I	GATC	1 cut(s)	439
LmnI	GCTCC	4 cut(s)	19, 241, 285, 588
Lsp1109I	GCAGC	6 cut(s)	132, 160, 364, 392, 526, 693
LweI	GCATC	4 cut(s)	70, 132, 397, 819
MaeI	CTAG	5 cut(s)	179, 420, 509, 516, 867
MaeII	ACGT	2 cut(s)	309, 768
MaeIII	GTNAC	1 cut(s)	643
MalI	GATC	1 cut(s)	441
MbiI	CCGCTC	2 cut(s)	288, 460
MboI	GATC	1 cut(s)	439
MboII	GAAGA	4 cut(s)	435, 654, 686, 773
MflI	RGATCY	1 cut(s)	439
MhlI	GDGCHC	1 cut(s)	24
MlsI	TGGCCA	2 cut(s)	5, 354
MluCI	AATT	2 cut(s)	48, 85
MluNI	TGGCCA	2 cut(s)	5, 354
Mox20I	TGGCCA	2 cut(s)	5, 354
MroI	TCCGGA	1 cut(s)	856
MroXI	GAANNNNTTC	1 cut(s)	510
MscI	TGGCCA	2 cut(s)	5, 354
Msp20I	TGGCCA	2 cut(s)	5, 354
MspA1I	CMGCKG	1 cut(s)	151
MspI	CCGG	5 cut(s)	114, 131, 171, 704, 857
MspR9I	CCNGG	1 cut(s)	26
MvaI	CCWGG	1 cut(s)	26
MwoI	GCNNNNNNNGC	3 cut(s)	89, 180, 189
NcoI	CCATGG	2 cut(s)	208, 349
NdeII	GATC	1 cut(s)	439
NlaIII	CATG	5 cut(s)	212, 353, 547, 607, 817
NlaIV	GGNNCC	5 cut(s)	21, 169, 206, 584, 636
NmeAIII	GCCGAG	3 cut(s)	33, 481, 812
NsbI	TGCGCA	1 cut(s)	467
PaeR7I	CTCGAG	1 cut(s)	41
PcsI	WCGNNNNNNNCGW	1 cut(s)	39
PdmI	GAANNNNTTC	1 cut(s)	510
PfeI	GAWTC	1 cut(s)	489
PflFI	GACNNNGTC	1 cut(s)	308
PkrI	GCNGC	7 cut(s)	147, 150, 379, 382, 541, 683, 778
Psp1406I	AACGTT	1 cut(s)	768
Psp6I	CCWGG	1 cut(s)	24
PspFI	CCCAGC	1 cut(s)	254
PspGI	CCWGG	1 cut(s)	24
PspN4I	GGNNCC	5 cut(s)	21, 169, 206, 584, 636
PspPI	GGNCC	1 cut(s)	168
PspXI	VCTCGAGB	1 cut(s)	41
PstI	CTGCAG	2 cut(s)	150, 382
PstNI	CAGNNNCTG	1 cut(s)	377
PsuI	RGATCY	1 cut(s)	439
PsyI	GACNNNGTC	1 cut(s)	308
PvuII	CAGCTG	1 cut(s)	151
SalI	GTCGAC	1 cut(s)	161
SatI	GCNGC	7 cut(s)	146, 149, 378, 381, 540, 682, 777
Sau3AI	GATC	1 cut(s)	439
Sau96I	GGNCC	1 cut(s)	168
ScrFI	CCNGG	1 cut(s)	26
SduI	GDGCHC	1 cut(s)	24
SfaNI	GCATC	4 cut(s)	70, 132, 397, 819
SfcI	CTRYAG	2 cut(s)	146, 378
Sfr274I	CTCGAG	1 cut(s)	41
SgrDI	CGTCGACG	1 cut(s)	161
SinI	GGWCC	1 cut(s)	168
SlaI	CTCGAG	1 cut(s)	41
SmlI	CTYRAG	1 cut(s)	41
SmoI	CTYRAG	1 cut(s)	41
SpeI	ACTAGT	1 cut(s)	508
Sse9I	AATT	2 cut(s)	48, 85
SspMI	CTAG	5 cut(s)	179, 420, 509, 516, 867
StyD4I	CCNGG	1 cut(s)	24
StyI	CCWWGG	5 cut(s)	178, 208, 225, 349, 407
TaaI	ACNGT	1 cut(s)	373
TaiI	ACGT	2 cut(s)	312, 771
TaqI	TCGA	4 cut(s)	42, 162, 301, 322
TasI	AATT	2 cut(s)	48, 85
TauI	GCSGC	1 cut(s)	779
TfiI	GAWTC	1 cut(s)	489
TscAI	CASTG	1 cut(s)	831
TseI	GCWGC	6 cut(s)	145, 148, 377, 380, 539, 681
TspDTI	ATGAA	2 cut(s)	318, 902
TspGWI	ACGGA	2 cut(s)	148, 545
TspRI	CASTG	1 cut(s)	831
Tth111I	GACNNNGTC	1 cut(s)	308
VpaK11BI	GGWCC	1 cut(s)	168
XapI	RAATTY	1 cut(s)	48
XhoI	CTCGAG	1 cut(s)	41
XmaJI	CCTAGG	1 cut(s)	178
XmiI	GTMKAC	1 cut(s)	162
XmnI	GAANNNNTTC	1 cut(s)	510
XspI	CTAG	5 cut(s)	179, 420, 509, 516, 867
ZraI	GACGTC	1 cut(s)	310

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.