pycom04g19790

Transcription factor UNE12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002134
Physical Location & Seq
Forward (+)
21429164 .. 21433508
4345 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g19790.1

Sequence Viewer

Length: 993 bp
ATGGCGGGAAATCCCCAAGAGGGACTTGGAGACGATTTCTTTGAGCAGATCTTAGCTGTGCCGCCGTCGTATAGTGCCGGCGGTGGTGGTGAGTCAGCTGGTGGTGGGTACGGAGGAGATGCGGGGTCCATGCCCATGGTGCTGCAGCTGGGTTCTGCTGGGTCTTCGGGTGGTGGTGCTGGGTACAGAGGAGGAGTTGGGATGGGGCTGGGTATGCCGTTGGGTTTGAACTTGGAGCAGGGTGGTTTTCTTGGGCGAGACAACAGGTTCAGAGATGAAGCCAACAATACTAACAACACAAACAGTTCCAATGTTTCTGCTTCAATTAATGAAAGAGAATCTGTTCATATGTCAAGTTTGTTTCCAGCATTTGGACACTTGCAAAATCACTCATCACTCCGTCCAACGCCACCACCTCCACAACCTCCTCACCAGTTTCATAGCCAACCACCACCAGGGCCGTCTAATGCTGTCCATCACCCTCCTTCCATCCGCCCAAGGGTTCGAGCAAGGCGAGGCCAAGCAACAGATCCCCACAGTATTGCTGAGCGGTTACGCCGGGAAAGAATTGCAGAGAGAATGAAGGCTTTGCAGGAATTGGTACCTAGTTGTAACAAGACAGATAGGGCAGCTATGCTTGATGAAATTGTGGATTACGTGAAATTTCTAAGGCTCCAAGTCAAGGTTTTAAGCATGAGTCGACTCGGGGGAGCAGGCGCAGTGGCTCAGCTTGTTGCCGACGTACCCCTATCAGCAGTCGAGGGGGAGGGCATGGAAGGAGGAACCAATCAACAAGCATGTGAGAAGTGGTCGAACGATGGCACAGAACAAGAAGTAGCTAAGCTGATGGAAGAAGATGTAGGAGCTGCCATGCAGTTCCTCCAGTCCAAGGCCCTCTGCATCATGCCCATATCCCTTGCCCCAGCAATTTTCCGGACACATCAACCAGACGCACCAACAATGGTGAAGCCGGAATCAAACTCCTCCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

331

Amino Acids

34.86

Weight (kDa)

5.86

Isoelectric Point (pI)

55.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 179 - 224 1.1e-08 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 183 - 227 4.6e-06 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 601
AccB1I GGYRCC 1 cut(s) 601
AccB7I CCANNNNNTGG 1 cut(s) 371
AccBSI CCGCTC 1 cut(s) 550
AccI GTMKAC 1 cut(s) 700
AccIII TCCGGA 1 cut(s) 933
AciI CCGC 6 cut(s) 5, 62, 81, 122, 493, 550
AclWI GGATC 1 cut(s) 524
AcsI RAATTY 1 cut(s) 662
AfaI GTAC 4 cut(s) 110, 185, 603, 744
AfiI CCNNNNNNNGG 6 cut(s) 20, 371, 455, 499, 682, 889
AgsI TTSAA 2 cut(s) 229, 324
AjnI CCWGG 1 cut(s) 454
AluBI AGCT 8 cut(s) 56, 98, 148, 632, 730, 839, 844, 866
AluI AGCT 8 cut(s) 56, 98, 148, 632, 730, 839, 844, 866
Alw26I GTCTC 2 cut(s) 24, 252
AlwI GGATC 1 cut(s) 524
Ama87I CYCGRG 1 cut(s) 704
Aor13HI TCCGGA 1 cut(s) 933
AoxI GGCC 3 cut(s) 458, 517, 891
ApeKI GCWGC 4 cut(s) 142, 145, 629, 866
ApoI RAATTY 1 cut(s) 662
ArsI GACNNNNNNTTYG 2 cut(s) 23, 55
AseI ATTAAT 1 cut(s) 327
Asp700I GAANNNNTTC 1 cut(s) 342
Asp718I GGTACC 1 cut(s) 601
AspLEI GCGC 1 cut(s) 719
AspS9I GGNCC 3 cut(s) 126, 458, 892
AsuC2I CCSGG 1 cut(s) 560
AsuHPI GGTGA 4 cut(s) 101, 422, 470, 976
AvaI CYCGRG 1 cut(s) 704
AvaII GGWCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 601
BbsI GAAGAC 1 cut(s) 156
BbvI GCAGC 4 cut(s) 129, 157, 641, 853
BccI CCATC 5 cut(s) 196, 483, 497, 812, 841
BceAI ACGGC 3 cut(s) 49, 202, 445
BciT130I CCWGG 1 cut(s) 456
BcnI CCSGG 1 cut(s) 560
BcoDI GTCTC 2 cut(s) 24, 252
BfaI CTAG 1 cut(s) 606
BfmI CTRYAG 1 cut(s) 143
BglII AGATCT 1 cut(s) 48
BisI GCNGC 5 cut(s) 62, 143, 146, 630, 867
BlpI GCTNAGC 3 cut(s) 546, 726, 840
BlsI GCNGC 5 cut(s) 63, 144, 147, 631, 868
Bme1390I CCNGG 2 cut(s) 456, 560
Bme18I GGWCC 1 cut(s) 126
BmeT110I CYCGRG 1 cut(s) 704
BmgT120I GGNCC 3 cut(s) 126, 458, 892
BmiI GGNNCC 4 cut(s) 127, 603, 674, 784
BmrFI CCNGG 2 cut(s) 456, 560
BmsI GCATC 2 cut(s) 109, 909
BpiI GAAGAC 1 cut(s) 156
BpmI CTGGAG 1 cut(s) 866
Bpu1102I GCTNAGC 3 cut(s) 546, 726, 840
BpuMI CCSGG 1 cut(s) 560
BsaAI YACGTR 1 cut(s) 658
BsaJI CCNNGG 4 cut(s) 135, 455, 497, 888
BsaWI WCCGGW 1 cut(s) 933
Bsc4I CCNNNNNNNGG 6 cut(s) 20, 371, 455, 499, 682, 889
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 2 cut(s) 433, 883
BseAI TCCGGA 1 cut(s) 933
BseBI CCWGG 1 cut(s) 456
BseDI CCNNGG 4 cut(s) 135, 455, 497, 888
BseGI GGATG 2 cut(s) 207, 489
BseLI CCNNNNNNNGG 6 cut(s) 20, 371, 455, 499, 682, 889
BseMII CTCAG 2 cut(s) 537, 740
BseNI ACTGG 2 cut(s) 433, 883
BseRI GAGGAG 6 cut(s) 129, 204, 207, 417, 973, 976
BseXI GCAGC 4 cut(s) 129, 157, 641, 853
BseYI CCCAGC 5 cut(s) 148, 158, 179, 208, 922
BshFI GGCC 3 cut(s) 460, 519, 893
BshNI GGYRCC 1 cut(s) 601
BsiHKCI CYCGRG 1 cut(s) 704
BsiSI CCGG 4 cut(s) 78, 559, 934, 971
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 6 cut(s) 20, 371, 455, 499, 682, 889
BsmAI GTCTC 2 cut(s) 24, 252
BsmBI CGTCTC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 36
BsnI GGCC 3 cut(s) 460, 519, 893
BsoBI CYCGRG 1 cut(s) 704
Bsp13I TCCGGA 1 cut(s) 933
Bsp143I GATC 2 cut(s) 48, 529
Bsp1720I GCTNAGC 3 cut(s) 546, 726, 840
Bsp19I CCATGG 1 cut(s) 135
BspACI CCGC 6 cut(s) 5, 62, 81, 122, 493, 550
BspANI GGCC 3 cut(s) 460, 519, 893
BspCNI CTCAG 2 cut(s) 538, 739
BspEI TCCGGA 1 cut(s) 933
BspLI GGNNCC 4 cut(s) 127, 603, 674, 784
BspMAI CTGCAG 1 cut(s) 147
BspPI GGATC 1 cut(s) 524
BspT107I GGYRCC 1 cut(s) 601
BsrBI CCGCTC 1 cut(s) 550
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 2 cut(s) 433, 883
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 4 cut(s) 135, 455, 497, 888
BssMI GATC 2 cut(s) 48, 529
BssT1I CCWWGG 3 cut(s) 135, 497, 888
Bst2UI CCWGG 1 cut(s) 456
Bst4CI ACNGT 2 cut(s) 305, 539
BstBAI YACGTR 1 cut(s) 658
BstC8I GCNNGC 2 cut(s) 79, 715
BstDEI CTNAG 5 cut(s) 52, 546, 668, 726, 840
BstDSI CCRYGG 1 cut(s) 135
BstF5I GGATG 2 cut(s) 207, 489
BstHHI GCGC 1 cut(s) 719
BstKTI GATC 2 cut(s) 51, 532
BstMAI GTCTC 2 cut(s) 24, 252
BstMBI GATC 2 cut(s) 48, 529
BstMWI GCNNNNNNNGC 2 cut(s) 139, 214
BstNI CCWGG 1 cut(s) 456
BstNSI RCATGY 1 cut(s) 801
BstSCI CCNGG 2 cut(s) 454, 558
BstSFI CTRYAG 1 cut(s) 143
BstV1I GCAGC 4 cut(s) 129, 157, 641, 853
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 2 cut(s) 48, 529
BstXI CCANNNNNNTGG 1 cut(s) 136
BstYI RGATCY 2 cut(s) 48, 529
BsuRI GGCC 3 cut(s) 460, 519, 893
BtgI CCRYGG 1 cut(s) 135
BtsCI GGATG 2 cut(s) 207, 489
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 1 cut(s) 726
Cac8I GCNNGC 2 cut(s) 79, 715
CfoI GCGC 1 cut(s) 719
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 3 cut(s) 126, 458, 892
CseI GACGC 1 cut(s) 959
Csp6I GTAC 4 cut(s) 109, 184, 602, 743
CviAII CATG 7 cut(s) 130, 136, 694, 772, 798, 871, 904
CviQI GTAC 4 cut(s) 109, 184, 602, 743
DdeI CTNAG 5 cut(s) 52, 546, 668, 726, 840
DpnI GATC 2 cut(s) 50, 531
DpnII GATC 2 cut(s) 48, 529
EciI GGCGGA 1 cut(s) 482
Eco130I CCWWGG 3 cut(s) 135, 497, 888
Eco47I GGWCC 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 704
EcoO109I RGGNCCY 1 cut(s) 892
EcoRII CCWGG 1 cut(s) 454
EcoT14I CCWWGG 3 cut(s) 135, 497, 888
ErhI CCWWGG 3 cut(s) 135, 497, 888
Esp3I CGTCTC 1 cut(s) 24
FaeI CATG 7 cut(s) 133, 139, 697, 775, 801, 874, 907
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 1 cut(s) 36
FatI CATG 7 cut(s) 129, 135, 693, 771, 797, 870, 903
FauI CCCGC 1 cut(s) 115
FauNDI CATATG 1 cut(s) 348
FblI GTMKAC 1 cut(s) 700
Fnu4HI GCNGC 5 cut(s) 62, 143, 146, 630, 867
FokI GGATG 2 cut(s) 214, 476
Fsp4HI GCNGC 5 cut(s) 62, 143, 146, 630, 867
FspBI CTAG 1 cut(s) 606
GlaI GCGC 1 cut(s) 718
GluI GCNGC 5 cut(s) 62, 143, 146, 630, 867
GsaI CCCAGC 5 cut(s) 152, 162, 183, 212, 926
GsuI CTGGAG 1 cut(s) 866
HaeIII GGCC 3 cut(s) 460, 519, 893
HapII CCGG 4 cut(s) 78, 559, 934, 971
HgaI GACGC 1 cut(s) 959
HhaI GCGC 1 cut(s) 719
Hin1II CATG 7 cut(s) 133, 139, 697, 775, 801, 874, 907
Hin6I GCGC 1 cut(s) 717
HinP1I GCGC 1 cut(s) 717
HincII GTYRAC 1 cut(s) 701
HindII GTYRAC 1 cut(s) 701
HinfI GANTC 5 cut(s) 92, 338, 697, 702, 974
HpaII CCGG 4 cut(s) 78, 559, 934, 971
HphI GGTGA 4 cut(s) 101, 422, 470, 976
Hpy166II GTNNAC 1 cut(s) 701
Hpy188I TCNGA 1 cut(s) 272
Hpy188III TCNNGA 1 cut(s) 934
Hpy8I GTNNAC 1 cut(s) 701
Hpy99I CGWCG 2 cut(s) 70, 743
HpyAV CCTTC 3 cut(s) 495, 577, 770
HpyCH4III ACNGT 2 cut(s) 305, 539
HpyCH4IV ACGT 2 cut(s) 657, 741
HpyCH4V TGCA 6 cut(s) 145, 382, 572, 592, 874, 900
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 214
HpyF3I CTNAG 5 cut(s) 52, 546, 668, 726, 840
HpySE526I ACGT 2 cut(s) 657, 741
Hsp92II CATG 7 cut(s) 133, 139, 697, 775, 801, 874, 907
HspAI GCGC 1 cut(s) 717
Kpn2I TCCGGA 1 cut(s) 933
KpnI GGTACC 1 cut(s) 605
KroI GCCGGC 1 cut(s) 77
KroNI GCCGGC 1 cut(s) 79
Kzo9I GATC 2 cut(s) 48, 529
LmnI GCTCC 4 cut(s) 235, 678, 710, 863
Lsp1109I GCAGC 4 cut(s) 129, 157, 641, 853
LweI GCATC 2 cut(s) 109, 909
MaeI CTAG 1 cut(s) 606
MaeII ACGT 2 cut(s) 657, 741
MaeIII GTNAC 2 cut(s) 552, 611
MalI GATC 2 cut(s) 50, 531
MbiI CCGCTC 1 cut(s) 550
MboI GATC 2 cut(s) 48, 529
MboII GAAGA 3 cut(s) 156, 863, 866
MflI RGATCY 2 cut(s) 48, 529
MluCI AATT 6 cut(s) 324, 567, 596, 645, 662, 927
MlyI GAGTC 3 cut(s) 101, 696, 706
MmeI TCCRAC 1 cut(s) 428
MroI TCCGGA 1 cut(s) 933
MroNI GCCGGC 1 cut(s) 77
MroXI GAANNNNTTC 1 cut(s) 342
MseI TTAA 2 cut(s) 327, 689
MslI CAYNNNNRTG 1 cut(s) 134
MspA1I CMGCKG 2 cut(s) 98, 148
MspI CCGG 4 cut(s) 78, 559, 934, 971
MspR9I CCNGG 2 cut(s) 456, 560
MvaI CCWGG 1 cut(s) 456
MwoI GCNNNNNNNGC 2 cut(s) 139, 214
NaeI GCCGGC 1 cut(s) 79
NciI CCSGG 1 cut(s) 560
NcoI CCATGG 1 cut(s) 135
NdeI CATATG 1 cut(s) 348
NdeII GATC 2 cut(s) 48, 529
NgoMIV GCCGGC 1 cut(s) 77
NlaIII CATG 7 cut(s) 133, 139, 697, 775, 801, 874, 907
NlaIV GGNNCC 4 cut(s) 127, 603, 674, 784
NspI RCATGY 1 cut(s) 801
PcsI WCGNNNNNNNCGW 1 cut(s) 511
PdiI GCCGGC 1 cut(s) 79
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 2 cut(s) 338, 974
PflMI CCANNNNNTGG 1 cut(s) 371
PkrI GCNGC 5 cut(s) 63, 144, 147, 631, 868
PleI GAGTC 3 cut(s) 100, 696, 705
PpsI GAGTC 3 cut(s) 100, 696, 705
Ppu21I YACGTR 1 cut(s) 658
PshBI ATTAAT 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 454
PspFI CCCAGC 5 cut(s) 148, 158, 179, 208, 922
PspGI CCWGG 1 cut(s) 454
PspN4I GGNNCC 4 cut(s) 127, 603, 674, 784
PspPI GGNCC 3 cut(s) 126, 458, 892
PstI CTGCAG 1 cut(s) 147
PsuI RGATCY 2 cut(s) 48, 529
PvuII CAGCTG 2 cut(s) 98, 148
RsaI GTAC 4 cut(s) 110, 185, 603, 744
RsaNI GTAC 4 cut(s) 109, 184, 602, 743
RseI CAYNNNNRTG 1 cut(s) 134
SalI GTCGAC 1 cut(s) 699
SaqAI TTAA 2 cut(s) 327, 689
SatI GCNGC 5 cut(s) 62, 143, 146, 630, 867
Sau3AI GATC 2 cut(s) 48, 529
Sau96I GGNCC 3 cut(s) 126, 458, 892
SchI GAGTC 3 cut(s) 101, 696, 706
ScrFI CCNGG 2 cut(s) 456, 560
SfaNI GCATC 2 cut(s) 109, 909
SfcI CTRYAG 1 cut(s) 143
SinI GGWCC 1 cut(s) 126
SmiMI CAYNNNNRTG 1 cut(s) 134
Sse9I AATT 6 cut(s) 324, 567, 596, 645, 662, 927
SsiI CCGC 6 cut(s) 5, 62, 81, 122, 493, 550
SspMI CTAG 1 cut(s) 606
StyD4I CCNGG 2 cut(s) 454, 558
StyI CCWWGG 3 cut(s) 135, 497, 888
TaaI ACNGT 2 cut(s) 305, 539
TaiI ACGT 2 cut(s) 660, 744
TaqI TCGA 4 cut(s) 505, 700, 759, 812
TasI AATT 6 cut(s) 324, 567, 596, 645, 662, 927
TauI GCSGC 1 cut(s) 64
TfiI GAWTC 2 cut(s) 338, 974
Tru1I TTAA 2 cut(s) 327, 689
Tru9I TTAA 2 cut(s) 327, 689
TscAI CASTG 1 cut(s) 726
TseI GCWGC 4 cut(s) 142, 145, 629, 866
TspDTI ATGAA 6 cut(s) 291, 335, 345, 428, 596, 657
TspGWI ACGGA 2 cut(s) 126, 389
TspRI CASTG 1 cut(s) 726
Van91I CCANNNNNTGG 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 126
VspI ATTAAT 1 cut(s) 327
XapI RAATTY 1 cut(s) 662
XceI RCATGY 1 cut(s) 801
XcmI CCANNNNNNNNNTGG 1 cut(s) 23
XmiI GTMKAC 1 cut(s) 700
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.