MD05G1296400.v1.1

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003875
Physical Location & Seq
Forward (+)
43078863 .. 43079570
708 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1296400.v1.1.491

Sequence Viewer

Length: 708 bp
ATGGAAGCAGACGAAGTAATGGAGCTATATGATTCTTTCTGGTTTGAGCTCCAAATCATCAACAAACAACCGATTTCACCAATCCCATCAAGGATTGAAGAAAACCCAGATCATGAAATCGAAGAAAACCCAGAAAAACCATCAAAGCCAGAGTTTTTACGCATTCCAACCAGGCACACCAGGTCCATGAGCGACGAGTTGAGCTCCAAAACGACCTTCAACTTGAGCTCTCTCTCGCCTGATTCTGTCCTCCACAGACCGAATCTCTCCACGGTTCTTTCCGGAAAAGAAGCCGGAGAAATCGAAAACCCGAAACAGAGATGTGCAGAAGAAGAAACACCGAAGAAAGTTGAAGGGAGGAGAAGAAAGAAGAAGAGTGAGAGCAAGAGCTTGACGGACCTTCAGTTTGAAGAGCTAAAAGGGTTTATGGATCTTGGTTTTGTTTTCTCAGAGGAAGATAAAGATTCAAACTTGGTTTCAATCATTCCTGGGCTGCAAAGATTGGGAAAGAAGGATGATAGCAGATATGAGAGTTTTGACGAGTCTGCAATTTCAAGGCCGTACCTTTCTGAAGCTTGGGAGGTTAGGGAGAAGAGGAAGAGAGAGAAGCCATTGATGAATTGGAGGTTTCCTGCTTTGGAGAATGAGATTGACATGAAGGACAATCTCAAATGGTGGGCTCATACTGTTGCTTCTGCAGTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

236

Amino Acids

27.4

Weight (kDa)

5.3

Isoelectric Point (pI)

64.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 131 - 165 6.1e-06 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 281
AclWI GGATC 1 cut(s) 438
AcuI CTGAAG 2 cut(s) 386, 591
AfaI GTAC 1 cut(s) 563
AgsI TTSAA 7 cut(s) 98, 220, 353, 410, 468, 480, 555
AjnI CCWGG 3 cut(s) 170, 179, 487
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 7 cut(s) 25, 49, 204, 228, 390, 415, 575
AluI AGCT 7 cut(s) 25, 49, 204, 228, 390, 415, 575
Alw21I GWGCWC 3 cut(s) 51, 206, 230
AlwI GGATC 1 cut(s) 438
Aor13HI TCCGGA 1 cut(s) 281
AoxI GGCC 1 cut(s) 557
ApeKI GCWGC 1 cut(s) 493
ArsI GACNNNNNNTTYG 2 cut(s) 389, 421
AspS9I GGNCC 2 cut(s) 183, 397
AsuHPI GGTGA 1 cut(s) 69
AvaII GGWCC 2 cut(s) 183, 397
BanII GRGCYC 4 cut(s) 51, 206, 230, 682
Bbv12I GWGCWC 3 cut(s) 51, 206, 230
BbvI GCAGC 1 cut(s) 480
BccI CCATC 2 cut(s) 94, 148
BceAI ACGGC 1 cut(s) 544
BciT130I CCWGG 3 cut(s) 172, 181, 489
BfmI CTRYAG 1 cut(s) 696
BisI GCNGC 1 cut(s) 494
BlsI GCNGC 1 cut(s) 495
Bme1390I CCNGG 3 cut(s) 172, 181, 489
Bme18I GGWCC 2 cut(s) 183, 397
BmgT120I GGNCC 2 cut(s) 183, 397
BmrFI CCNGG 3 cut(s) 172, 181, 489
BplI GAGNNNNNCTC 2 cut(s) 188, 220
BpuEI CTTGAG 1 cut(s) 244
BsaJI CCNNGG 2 cut(s) 270, 488
BsaWI WCCGGW 1 cut(s) 281
BseAI TCCGGA 1 cut(s) 281
BseBI CCWGG 3 cut(s) 172, 181, 489
BseDI CCNNGG 2 cut(s) 270, 488
BseGI GGATG 1 cut(s) 520
BseMII CTCAG 1 cut(s) 462
BseRI GAGGAG 1 cut(s) 373
BseXI GCAGC 1 cut(s) 480
BsgI GTGCAG 1 cut(s) 345
BshFI GGCC 1 cut(s) 559
BsiHKAI GWGCWC 3 cut(s) 51, 206, 230
BsiSI CCGG 2 cut(s) 282, 294
BsmI GAATGC 1 cut(s) 162
BsnI GGCC 1 cut(s) 559
Bsp1286I GDGCHC 4 cut(s) 51, 206, 230, 682
Bsp13I TCCGGA 1 cut(s) 281
Bsp143I GATC 2 cut(s) 109, 430
BspANI GGCC 1 cut(s) 559
BspCNI CTCAG 1 cut(s) 461
BspEI TCCGGA 1 cut(s) 281
BspHI TCATGA 1 cut(s) 112
BspMAI CTGCAG 1 cut(s) 700
BspPI GGATC 1 cut(s) 438
BspQI GCTCTTC 1 cut(s) 405
BssECI CCNNGG 2 cut(s) 270, 488
BssMI GATC 2 cut(s) 109, 430
Bst2UI CCWGG 3 cut(s) 172, 181, 489
Bst4CI ACNGT 2 cut(s) 274, 688
Bst6I CTCTTC 4 cut(s) 368, 405, 587, 593
BstDEI CTNAG 1 cut(s) 448
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 1 cut(s) 520
BstKTI GATC 2 cut(s) 112, 433
BstMBI GATC 2 cut(s) 109, 430
BstNI CCWGG 3 cut(s) 172, 181, 489
BstSCI CCNGG 3 cut(s) 170, 179, 487
BstSFI CTRYAG 1 cut(s) 696
BstV1I GCAGC 1 cut(s) 480
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
BsuRI GGCC 1 cut(s) 559
BtgI CCRYGG 1 cut(s) 270
BtsCI GGATG 1 cut(s) 520
CciI TCATGA 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 183, 397
CsiI ACCWGGT 1 cut(s) 179
Csp6I GTAC 1 cut(s) 562
CviAII CATG 3 cut(s) 113, 187, 655
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 1 cut(s) 448
DpnI GATC 2 cut(s) 111, 432
DpnII GATC 2 cut(s) 109, 430
Eam1104I CTCTTC 4 cut(s) 368, 405, 587, 593
EarI CTCTTC 4 cut(s) 368, 405, 587, 593
Ecl136II GAGCTC 3 cut(s) 49, 204, 228
Eco24I GRGCYC 4 cut(s) 51, 206, 230, 682
Eco47I GGWCC 2 cut(s) 183, 397
Eco53kI GAGCTC 3 cut(s) 49, 204, 228
Eco57I CTGAAG 2 cut(s) 386, 591
EcoICRI GAGCTC 3 cut(s) 49, 204, 228
EcoRII CCWGG 3 cut(s) 170, 179, 487
EcoT38I GRGCYC 4 cut(s) 51, 206, 230, 682
FaeI CATG 3 cut(s) 116, 190, 658
FaiI YATR 8 cut(s) 28, 30, 114, 188, 428, 528, 656, 684
FatI CATG 3 cut(s) 112, 186, 654
Fnu4HI GCNGC 1 cut(s) 494
FokI GGATG 1 cut(s) 527
FriOI GRGCYC 4 cut(s) 51, 206, 230, 682
Fsp4HI GCNGC 1 cut(s) 494
GluI GCNGC 1 cut(s) 494
HaeIII GGCC 1 cut(s) 559
HapII CCGG 2 cut(s) 282, 294
Hin1II CATG 3 cut(s) 116, 190, 658
HindIII AAGCTT 1 cut(s) 573
HinfI GANTC 5 cut(s) 32, 242, 262, 464, 542
HpaII CCGG 2 cut(s) 282, 294
HphI GGTGA 1 cut(s) 69
Hpy188I TCNGA 2 cut(s) 451, 571
Hpy188III TCNNGA 2 cut(s) 113, 282
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 5 cut(s) 226, 347, 410, 505, 652
HpyCH4III ACNGT 2 cut(s) 274, 688
HpyCH4V TGCA 4 cut(s) 326, 496, 548, 698
HpyF3I CTNAG 1 cut(s) 448
Hsp92II CATG 3 cut(s) 116, 190, 658
Kpn2I TCCGGA 1 cut(s) 281
Kzo9I GATC 2 cut(s) 109, 430
LguI GCTCTTC 1 cut(s) 405
LmnI GCTCC 3 cut(s) 22, 54, 209
Lsp1109I GCAGC 1 cut(s) 480
MabI ACCWGGT 1 cut(s) 179
MalI GATC 2 cut(s) 111, 432
MboI GATC 2 cut(s) 109, 430
MflI RGATCY 1 cut(s) 430
MhlI GDGCHC 4 cut(s) 51, 206, 230, 682
MluCI AATT 2 cut(s) 549, 619
MlyI GAGTC 1 cut(s) 551
MmeI TCCRAC 1 cut(s) 191
MnlI CCTC 6 cut(s) 260, 351, 445, 574, 588, 618
MroI TCCGGA 1 cut(s) 281
MspI CCGG 2 cut(s) 282, 294
MspR9I CCNGG 3 cut(s) 172, 181, 489
Mva1269I GAATGC 1 cut(s) 162
MvaI CCWGG 3 cut(s) 172, 181, 489
NdeII GATC 2 cut(s) 109, 430
NlaIII CATG 3 cut(s) 116, 190, 658
PagI TCATGA 1 cut(s) 112
PciSI GCTCTTC 1 cut(s) 405
PctI GAATGC 1 cut(s) 162
PfeI GAWTC 4 cut(s) 32, 242, 262, 464
PkrI GCNGC 1 cut(s) 495
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
Psp124BI GAGCTC 3 cut(s) 51, 206, 230
Psp6I CCWGG 3 cut(s) 170, 179, 487
PspGI CCWGG 3 cut(s) 170, 179, 487
PspPI GGNCC 2 cut(s) 183, 397
PstI CTGCAG 1 cut(s) 700
PsuI RGATCY 1 cut(s) 430
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
SacI GAGCTC 3 cut(s) 51, 206, 230
SapI GCTCTTC 1 cut(s) 405
SatI GCNGC 1 cut(s) 494
Sau3AI GATC 2 cut(s) 109, 430
Sau96I GGNCC 2 cut(s) 183, 397
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 3 cut(s) 172, 181, 489
SduI GDGCHC 4 cut(s) 51, 206, 230, 682
SexAI ACCWGGT 1 cut(s) 179
SfcI CTRYAG 1 cut(s) 696
SinI GGWCC 2 cut(s) 183, 397
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
Sse9I AATT 2 cut(s) 549, 619
SstI GAGCTC 3 cut(s) 51, 206, 230
StyD4I CCNGG 3 cut(s) 170, 179, 487
TaaI ACNGT 2 cut(s) 274, 688
TaqI TCGA 2 cut(s) 120, 303
TaqII GACCGA 1 cut(s) 274
TasI AATT 2 cut(s) 549, 619
TfiI GAWTC 4 cut(s) 32, 242, 262, 464
TseI GCWGC 1 cut(s) 493
TspDTI ATGAA 3 cut(s) 129, 632, 671
TspGWI ACGGA 1 cut(s) 410
VpaK11BI GGWCC 2 cut(s) 183, 397
XcmI CCANNNNNNNNNTGG 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.