Rorug04G0457500

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003875
Physical Location & Seq
Reverse (-)
61053489 .. 61054461
973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0457500.1

Sequence Viewer

Length: 516 bp
ATGGTCGATTCAGCTCAAGATATTGAGATTGATTCGAAATCGGCTCAAACGGAGCCAGGTCCTCCGCCGTCACTACAGTCCATGTTGCGGCGCAAGGATGCCACCGGCGGCTGCGCAAGGTCTAGGCGAAGCTACTGGAGGTGGTCCGACGTCTTGAGGTGGCCTGAGGAGAGAGAGGTCGGCAGGGTAGAATTTGTGATACCCAGTCCAGGTCTGCATTTGGCTCTCAATGCTTGTGCAGCAGCGGCAGTTGCAACTCTTTTAGGAGTTTCTCTTTCTCAAGTTGGAATTTGTCTATCAGCATTTACTCCCGTCAAAATGAGGTCAGAGCTTGAAGTTGCTAGAAATGGAGTCAAGATAGTCAATGATGTTTACAATGCCAATCCTGTGAGCTCCAGAGCTGCCATTGACTTATTGAAGAGTATTGATTGCAAGGGCCAAAAAATTGCCATACTTGGTGACATGTTAGAACTCGGGGCCATTGAGTACGTTGGAGTCTCACAAGATGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

171

Amino Acids

18.44

Weight (kDa)

5.48

Isoelectric Point (pI)

69.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mur_ligase_C PF02875 108 - 159 7.6e-07 Mur ligase, glutamate ligase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 153
Acc16I TGCGCA 1 cut(s) 115
AciI CCGC 4 cut(s) 65, 88, 108, 245
AcsI RAATTY 2 cut(s) 191, 288
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 1 cut(s) 488
AfiI CCNNNNNNNGG 2 cut(s) 87, 209
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 2 cut(s) 335, 418
AjnI CCWGG 2 cut(s) 55, 208
AluBI AGCT 5 cut(s) 14, 132, 331, 393, 401
AluI AGCT 5 cut(s) 14, 132, 331, 393, 401
Alw21I GWGCWC 1 cut(s) 395
Alw26I GTCTC 1 cut(s) 502
Ama87I CYCGRG 1 cut(s) 473
AoxI GGCC 3 cut(s) 161, 436, 477
ApeKI GCWGC 4 cut(s) 111, 239, 242, 401
ApoI RAATTY 2 cut(s) 191, 288
AspLEI GCGC 2 cut(s) 93, 116
AspS9I GGNCC 4 cut(s) 59, 144, 436, 477
AsuHPI GGTGA 1 cut(s) 470
AsuII TTCGAA 1 cut(s) 35
AvaI CYCGRG 1 cut(s) 473
AvaII GGWCC 2 cut(s) 59, 144
AxyI CCTNAGG 1 cut(s) 165
BanII GRGCYC 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 395
BbvI GCAGC 4 cut(s) 98, 251, 254, 388
BceAI ACGGC 1 cut(s) 52
BciT130I CCWGG 2 cut(s) 57, 210
BcoDI GTCTC 1 cut(s) 502
BfaI CTAG 2 cut(s) 123, 342
BfmI CTRYAG 1 cut(s) 74
BisI GCNGC 7 cut(s) 89, 109, 112, 240, 243, 246, 402
BlsI GCNGC 7 cut(s) 90, 110, 113, 241, 244, 247, 403
Bme1390I CCNGG 2 cut(s) 57, 210
Bme18I GGWCC 2 cut(s) 59, 144
BmeT110I CYCGRG 1 cut(s) 473
BmgT120I GGNCC 4 cut(s) 59, 144, 436, 477
BmiI GGNNCC 2 cut(s) 54, 478
BmrFI CCNGG 2 cut(s) 57, 210
BmrI ACTGGG 1 cut(s) 198
BmsI GCATC 1 cut(s) 88
BmuI ACTGGG 1 cut(s) 198
BpmI CTGGAG 2 cut(s) 157, 379
Bpu14I TTCGAA 1 cut(s) 35
BpuEI CTTGAG 2 cut(s) 175, 264
BsaHI GRCGYC 1 cut(s) 150
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 209
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 140, 204
Bse21I CCTNAGG 1 cut(s) 165
BseBI CCWGG 2 cut(s) 57, 210
BseGI GGATG 1 cut(s) 103
BseLI CCNNNNNNNGG 2 cut(s) 87, 209
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 2 cut(s) 140, 204
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 4 cut(s) 98, 251, 254, 388
BsgI GTGCAG 1 cut(s) 258
BshFI GGCC 3 cut(s) 163, 438, 479
BsiHKAI GWGCWC 1 cut(s) 395
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 1 cut(s) 105
BslI CCNNNNNNNGG 2 cut(s) 87, 209
BsmAI GTCTC 1 cut(s) 502
BsnI GGCC 3 cut(s) 163, 438, 479
BsoBI CYCGRG 1 cut(s) 473
Bsp119I TTCGAA 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 395
BspACI CCGC 4 cut(s) 65, 88, 108, 245
BspANI GGCC 3 cut(s) 163, 438, 479
BspCNI CTCAG 1 cut(s) 157
BspLI GGNNCC 2 cut(s) 54, 478
BspT104I TTCGAA 1 cut(s) 35
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 2 cut(s) 140, 204
BssAI RCCGGY 1 cut(s) 104
BssNI GRCGYC 1 cut(s) 150
Bst2UI CCWGG 2 cut(s) 57, 210
Bst4CI ACNGT 1 cut(s) 78
Bst6I CTCTTC 1 cut(s) 413
BstACI GRCGYC 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 35
BstDEI CTNAG 1 cut(s) 165
BstF5I GGATG 1 cut(s) 103
BstHHI GCGC 2 cut(s) 93, 116
BstMAI GTCTC 1 cut(s) 502
BstMWI GCNNNNNNNGC 4 cut(s) 230, 239, 245, 251
BstNI CCWGG 2 cut(s) 57, 210
BstNSI RCATGY 1 cut(s) 466
BstSCI CCNGG 2 cut(s) 55, 208
BstSFI CTRYAG 1 cut(s) 74
BstV1I GCAGC 4 cut(s) 98, 251, 254, 388
Bsu36I CCTNAGG 1 cut(s) 165
BsuRI GGCC 3 cut(s) 163, 438, 479
BtsCI GGATG 1 cut(s) 103
CfoI GCGC 2 cut(s) 93, 116
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 4 cut(s) 59, 144, 436, 477
Csp6I GTAC 1 cut(s) 487
CviAII CATG 2 cut(s) 82, 463
CviQI GTAC 1 cut(s) 487
DdeI CTNAG 1 cut(s) 165
Eam1104I CTCTTC 1 cut(s) 413
EarI CTCTTC 1 cut(s) 413
EciI GGCGGA 1 cut(s) 54
Ecl136II GAGCTC 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 395
Eco47I GGWCC 2 cut(s) 59, 144
Eco53kI GAGCTC 1 cut(s) 393
Eco81I CCTNAGG 1 cut(s) 165
Eco88I CYCGRG 1 cut(s) 473
EcoICRI GAGCTC 1 cut(s) 393
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 2 cut(s) 55, 208
EcoT38I GRGCYC 1 cut(s) 395
FaeI CATG 2 cut(s) 85, 466
FaiI YATR 3 cut(s) 83, 452, 464
FatI CATG 2 cut(s) 81, 462
Fnu4HI GCNGC 7 cut(s) 89, 109, 112, 240, 243, 246, 402
FokI GGATG 1 cut(s) 110
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 7 cut(s) 89, 109, 112, 240, 243, 246, 402
FspBI CTAG 2 cut(s) 123, 342
FspI TGCGCA 1 cut(s) 115
GlaI GCGC 2 cut(s) 92, 115
GluI GCNGC 7 cut(s) 89, 109, 112, 240, 243, 246, 402
GsuI CTGGAG 2 cut(s) 157, 379
HaeIII GGCC 3 cut(s) 163, 438, 479
HapII CCGG 1 cut(s) 105
HhaI GCGC 2 cut(s) 93, 116
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 2 cut(s) 85, 466
Hin6I GCGC 2 cut(s) 91, 114
HinP1I GCGC 2 cut(s) 91, 114
HinfI GANTC 5 cut(s) 8, 32, 351, 495, 509
HpaII CCGG 1 cut(s) 105
HphI GGTGA 1 cut(s) 470
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 148, 328
Hpy188III TCNNGA 4 cut(s) 17, 154, 355, 396
Hpy8I GTNNAC 1 cut(s) 373
Hpy99I CGWCG 1 cut(s) 152
HpyCH4III ACNGT 1 cut(s) 78
HpyCH4IV ACGT 2 cut(s) 150, 489
HpyCH4V TGCA 4 cut(s) 217, 239, 254, 432
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 239, 245, 251
HpyF3I CTNAG 1 cut(s) 165
HpySE526I ACGT 2 cut(s) 150, 489
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 2 cut(s) 85, 466
HspAI GCGC 2 cut(s) 91, 114
LmnI GCTCC 2 cut(s) 52, 398
Lsp1109I GCAGC 4 cut(s) 98, 251, 254, 388
LweI GCATC 1 cut(s) 88
MaeI CTAG 2 cut(s) 123, 342
MaeII ACGT 2 cut(s) 150, 489
MaeIII GTNAC 2 cut(s) 69, 458
MboII GAAGA 1 cut(s) 430
MhlI GDGCHC 1 cut(s) 395
MluCI AATT 3 cut(s) 191, 288, 444
MlyI GAGTC 2 cut(s) 360, 504
MmeI TCCRAC 3 cut(s) 171, 265, 472
MnlI CCTC 6 cut(s) 72, 132, 150, 160, 169, 315
MseI TTAA 1 cut(s) 514
MspA1I CMGCKG 1 cut(s) 245
MspI CCGG 1 cut(s) 105
MspR9I CCNGG 2 cut(s) 57, 210
MvaI CCWGG 2 cut(s) 57, 210
MwoI GCNNNNNNNGC 4 cut(s) 230, 239, 245, 251
NlaIII CATG 2 cut(s) 85, 466
NlaIV GGNNCC 2 cut(s) 54, 478
NmuCI GTSAC 2 cut(s) 69, 458
NsbI TGCGCA 1 cut(s) 115
NspI RCATGY 1 cut(s) 466
NspV TTCGAA 1 cut(s) 35
PciI ACATGT 1 cut(s) 462
PfeI GAWTC 3 cut(s) 8, 32, 509
PkrI GCNGC 7 cut(s) 90, 110, 113, 241, 244, 247, 403
PleI GAGTC 2 cut(s) 359, 503
PpsI GAGTC 2 cut(s) 359, 503
PpuMI RGGWCCY 1 cut(s) 59
PscI ACATGT 1 cut(s) 462
Psp124BI GAGCTC 1 cut(s) 395
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 2 cut(s) 55, 208
PspGI CCWGG 2 cut(s) 55, 208
PspN4I GGNNCC 2 cut(s) 54, 478
PspPI GGNCC 4 cut(s) 59, 144, 436, 477
PspPPI RGGWCCY 1 cut(s) 59
RsaI GTAC 1 cut(s) 488
RsaNI GTAC 1 cut(s) 487
SacI GAGCTC 1 cut(s) 395
SaqAI TTAA 1 cut(s) 514
SatI GCNGC 7 cut(s) 89, 109, 112, 240, 243, 246, 402
Sau96I GGNCC 4 cut(s) 59, 144, 436, 477
SchI GAGTC 2 cut(s) 360, 504
ScrFI CCNGG 2 cut(s) 57, 210
SduI GDGCHC 1 cut(s) 395
SfaNI GCATC 1 cut(s) 88
SfcI CTRYAG 1 cut(s) 74
SfuI TTCGAA 1 cut(s) 35
SgrAI CRCCGGYG 1 cut(s) 104
SinI GGWCC 2 cut(s) 59, 144
SmlI CTYRAG 3 cut(s) 15, 154, 279
SmoI CTYRAG 3 cut(s) 15, 154, 279
Sse9I AATT 3 cut(s) 191, 288, 444
SsiI CCGC 4 cut(s) 65, 88, 108, 245
SspMI CTAG 2 cut(s) 123, 342
SstI GAGCTC 1 cut(s) 395
StyD4I CCNGG 2 cut(s) 55, 208
TaaI ACNGT 1 cut(s) 78
TaiI ACGT 2 cut(s) 153, 492
TaqI TCGA 2 cut(s) 6, 35
TasI AATT 3 cut(s) 191, 288, 444
TauI GCSGC 3 cut(s) 91, 111, 248
TfiI GAWTC 3 cut(s) 8, 32, 509
Tru1I TTAA 1 cut(s) 514
Tru9I TTAA 1 cut(s) 514
TseFI GTSAC 2 cut(s) 69, 458
TseI GCWGC 4 cut(s) 111, 239, 242, 401
Tsp45I GTSAC 2 cut(s) 69, 458
TspGWI ACGGA 1 cut(s) 65
VpaK11BI GGWCC 2 cut(s) 59, 144
XapI RAATTY 2 cut(s) 191, 288
XceI RCATGY 1 cut(s) 466
XspI CTAG 2 cut(s) 123, 342
ZraI GACGTC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.