Rh5AG089300

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003875
Physical Location & Seq
Reverse (-)
7943547 .. 7945202
1656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG089300.1

Sequence Viewer

Length: 717 bp
ATGGAAGCAGAGCAAATCATGGAGCTCTTTGATTCTTACTGGTTTGAGCTTGAAATCCTCAAGAAACAACCATTTTCAGCAAAGTCATCAAGATTTGAAGCTGCCCCAGTTCATGAAATTGAAGAACAACCATCAAAACCAGAGGTCTTGCGCATTCCAAGCCTCCATACTAGATCCATGAGCGAAGATTTGAGCTCCAAAACGAGCTTTATGAATTGCGGTTCTCCCTCGCCAGACTCAGTCCTCCACAGACCAAAGCTCACCACCATTATTTCTGGAAAAGAAGCCACAGAATCAATGGAAGATATTCCAGTACAAAAACATGAAACAGAGTCATCGAAGAAGGCTAAAATTGAGAGAAGAAGGAAGAAGGGCGGGAGCAAGAGCTTGACAGACCTTCAGTTTGAGGAGCTAAAAGGGTTTATGGATTTGGGATTTGTTTTCTCAGAAGAAGACAAAGACTCAAACTTGGCTTCAATCATTCCTGGTCTGAAAAAATTGGGGAAGAAAGATGATGGTGAAGTAGACGAGGAAGCTTTTGATGATGAGTCTGCGATTTCAAGGCCTTATCTTTCTGAAGCGTGGGAGGTGATGGAGAAGAGAAAGAAAGAGAAGAAGCCATTGATGGATTGGAGGTTTCCATCTTTGAGCAATGAAATTGACATGAAGGACAATCTCAAATGGTGGGCTCATACTGTTGCTTCTACAGTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

238

Amino Acids

27.32

Weight (kDa)

5.37

Isoelectric Point (pI)

59.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 130 - 164 9.3e-06 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 152
AccI GTMKAC 1 cut(s) 525
AciI CCGC 2 cut(s) 219, 375
AclWI GGATC 1 cut(s) 168
AcuI CTGAAG 2 cut(s) 383, 597
AfaI GTAC 1 cut(s) 315
AgsI TTSAA 5 cut(s) 53, 98, 122, 477, 561
AjnI CCWGG 1 cut(s) 484
AluBI AGCT 9 cut(s) 25, 49, 101, 195, 207, 259, 387, 412, 536
AluI AGCT 9 cut(s) 25, 49, 101, 195, 207, 259, 387, 412, 536
Alw21I GWGCWC 2 cut(s) 27, 197
AlwI GGATC 1 cut(s) 168
AoxI GGCC 1 cut(s) 563
ApeKI GCWGC 1 cut(s) 101
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
Asp700I GAANNNNTTC 1 cut(s) 306
AspLEI GCGC 1 cut(s) 153
AsuHPI GGTGA 3 cut(s) 253, 530, 601
BanII GRGCYC 3 cut(s) 27, 197, 691
BbsI GAAGAC 1 cut(s) 459
Bbv12I GWGCWC 2 cut(s) 27, 197
BbvI GCAGC 1 cut(s) 88
BccI CCATC 5 cut(s) 139, 509, 586, 619, 649
BciT130I CCWGG 1 cut(s) 486
BfaI CTAG 1 cut(s) 171
BfmI CTRYAG 1 cut(s) 705
BisI GCNGC 1 cut(s) 102
BlsI GCNGC 1 cut(s) 103
Bme1390I CCNGG 1 cut(s) 486
BmrFI CCNGG 1 cut(s) 486
BmrI ACTGGG 1 cut(s) 101
BmuI ACTGGG 1 cut(s) 101
BpiI GAAGAC 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 44
Bse1I ACTGG 3 cut(s) 44, 107, 311
Bse3DI GCAATG 1 cut(s) 658
BseBI CCWGG 1 cut(s) 486
BseMI GCAATG 1 cut(s) 658
BseMII CTCAG 2 cut(s) 252, 459
BseNI ACTGG 3 cut(s) 44, 107, 311
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 1 cut(s) 88
BshFI GGCC 1 cut(s) 565
BsiHKAI GWGCWC 2 cut(s) 27, 197
BsmI GAATGC 1 cut(s) 153
BsnI GGCC 1 cut(s) 565
Bsp1286I GDGCHC 3 cut(s) 27, 197, 691
Bsp143I GATC 1 cut(s) 173
BspACI CCGC 2 cut(s) 219, 375
BspANI GGCC 1 cut(s) 565
BspCNI CTCAG 2 cut(s) 251, 458
BspHI TCATGA 1 cut(s) 112
BspPI GGATC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 658
BsrI ACTGG 3 cut(s) 44, 107, 311
BssMI GATC 1 cut(s) 173
Bst2UI CCWGG 1 cut(s) 486
Bst4CI ACNGT 2 cut(s) 697, 709
Bst6I CTCTTC 1 cut(s) 593
BstDEI CTNAG 2 cut(s) 238, 445
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 1 cut(s) 176
BstMBI GATC 1 cut(s) 173
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNI CCWGG 1 cut(s) 486
BstSCI CCNGG 1 cut(s) 484
BstSFI CTRYAG 1 cut(s) 705
BstV1I GCAGC 1 cut(s) 88
BstV2I GAAGAC 1 cut(s) 459
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
BsuRI GGCC 1 cut(s) 565
CciI TCATGA 1 cut(s) 112
CfoI GCGC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 314
CviAII CATG 5 cut(s) 19, 113, 178, 323, 664
CviQI GTAC 1 cut(s) 314
DdeI CTNAG 2 cut(s) 238, 445
DpnI GATC 1 cut(s) 175
DpnII GATC 1 cut(s) 173
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
Ecl136II GAGCTC 2 cut(s) 25, 195
Eco147I AGGCCT 1 cut(s) 565
Eco24I GRGCYC 3 cut(s) 27, 197, 691
Eco53kI GAGCTC 2 cut(s) 25, 195
Eco57I CTGAAG 2 cut(s) 383, 597
EcoICRI GAGCTC 2 cut(s) 25, 195
EcoRII CCWGG 1 cut(s) 484
EcoT38I GRGCYC 3 cut(s) 27, 197, 691
FaeI CATG 5 cut(s) 22, 116, 181, 326, 667
FaiI YATR 9 cut(s) 20, 114, 168, 179, 212, 324, 425, 665, 693
FatI CATG 5 cut(s) 18, 112, 177, 322, 663
FauI CCCGC 1 cut(s) 368
FblI GTMKAC 1 cut(s) 525
Fnu4HI GCNGC 1 cut(s) 102
FriOI GRGCYC 3 cut(s) 27, 197, 691
Fsp4HI GCNGC 1 cut(s) 102
FspBI CTAG 1 cut(s) 171
FspI TGCGCA 1 cut(s) 152
GlaI GCGC 1 cut(s) 152
GluI GCNGC 1 cut(s) 102
HaeIII GGCC 1 cut(s) 565
HhaI GCGC 1 cut(s) 153
Hin1II CATG 5 cut(s) 22, 116, 181, 326, 667
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HindIII AAGCTT 1 cut(s) 534
HinfI GANTC 6 cut(s) 32, 236, 293, 332, 461, 548
HphI GGTGA 3 cut(s) 253, 530, 601
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 3 cut(s) 448, 492, 577
Hpy188III TCNNGA 4 cut(s) 61, 90, 113, 276
Hpy8I GTNNAC 1 cut(s) 526
HpyAV CCTTC 5 cut(s) 337, 357, 364, 407, 661
HpyCH4III ACNGT 2 cut(s) 697, 709
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
HpyF3I CTNAG 2 cut(s) 238, 445
Hsp92II CATG 5 cut(s) 22, 116, 181, 326, 667
HspAI GCGC 1 cut(s) 151
Kzo9I GATC 1 cut(s) 173
LmnI GCTCC 4 cut(s) 22, 200, 378, 409
LpnPI CCDG 8 cut(s) 25, 120, 153, 246, 261, 324, 471, 498
Lsp1109I GCAGC 1 cut(s) 88
MaeI CTAG 1 cut(s) 171
MalI GATC 1 cut(s) 175
MboI GATC 1 cut(s) 173
MflI RGATCY 1 cut(s) 173
MhlI GDGCHC 3 cut(s) 27, 197, 691
MluCI AATT 5 cut(s) 117, 214, 351, 497, 657
MlyI GAGTC 4 cut(s) 230, 341, 455, 557
MnlI CCTC 9 cut(s) 68, 136, 173, 238, 254, 400, 523, 580, 627
MroXI GAANNNNTTC 1 cut(s) 306
MspR9I CCNGG 1 cut(s) 486
Mva1269I GAATGC 1 cut(s) 153
MvaI CCWGG 1 cut(s) 486
MwoI GCNNNNNNNGC 1 cut(s) 159
NdeII GATC 1 cut(s) 173
NlaIII CATG 5 cut(s) 22, 116, 181, 326, 667
NsbI TGCGCA 1 cut(s) 152
PagI TCATGA 1 cut(s) 112
PceI AGGCCT 1 cut(s) 565
PctI GAATGC 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 2 cut(s) 32, 293
PflFI GACNNNGTC 1 cut(s) 239
PkrI GCNGC 1 cut(s) 103
PleI GAGTC 4 cut(s) 230, 340, 455, 556
PpsI GAGTC 4 cut(s) 230, 340, 455, 556
Psp124BI GAGCTC 2 cut(s) 27, 197
Psp6I CCWGG 1 cut(s) 484
PspGI CCWGG 1 cut(s) 484
PsuI RGATCY 1 cut(s) 173
PsyI GACNNNGTC 1 cut(s) 239
RsaI GTAC 1 cut(s) 315
RsaNI GTAC 1 cut(s) 314
SacI GAGCTC 2 cut(s) 27, 197
SatI GCNGC 1 cut(s) 102
Sau3AI GATC 1 cut(s) 173
SchI GAGTC 4 cut(s) 230, 341, 455, 557
ScrFI CCNGG 1 cut(s) 486
SduI GDGCHC 3 cut(s) 27, 197, 691
SfcI CTRYAG 1 cut(s) 705
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 5 cut(s) 117, 214, 351, 497, 657
SseBI AGGCCT 1 cut(s) 565
SsiI CCGC 2 cut(s) 219, 375
SspMI CTAG 1 cut(s) 171
SstI GAGCTC 2 cut(s) 27, 197
StuI AGGCCT 1 cut(s) 565
StyD4I CCNGG 1 cut(s) 484
TaaI ACNGT 2 cut(s) 697, 709
TaqI TCGA 1 cut(s) 338
TasI AATT 5 cut(s) 117, 214, 351, 497, 657
TatI WGTACW 1 cut(s) 313
TfiI GAWTC 2 cut(s) 32, 293
TseI GCWGC 1 cut(s) 101
TspDTI ATGAA 6 cut(s) 101, 129, 227, 339, 669, 680
Tth111I GACNNNGTC 1 cut(s) 239
XcmI CCANNNNNNNNNTGG 2 cut(s) 295, 627
XmiI GTMKAC 1 cut(s) 525
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.