pycom10g23080

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003875
Physical Location & Seq
Forward (+)
25339153 .. 25340005
853 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g23080.1

Sequence Viewer

Length: 600 bp
ATGGAAAACGGAAGCAGACGAAAAAACCCATCAAAGCCAGAGTTTTTACGGATTCCAACCAGGCACACCAGGTCCATGAGCGACGAGTTGAGCTCCAATACGAGCTTCAACTTGAGCACTCTCTCGCCGGACTCTGTCCTCCACAGAACGAAGCTCTCCACAATTCTTTCCGGAAAAGAAGCCAGGGAAATCGGAAACCCGAAACAGATATATTCAGAAGAAGAACCACAGAAGAAAGTTGAAAGGAGGAGGAGAAGAAAGAAGAAGAGTGAGAGCAAGAGCTTGACGGACCTTCAGTTTGAAGAGCTAAAAGGGTTTATGGATCTTGGTTTTGTTTTCTCAGAGGAAGATAAAGATTCAAACTTGGTTTCAATCATTCCTGGGCTGCAAAGATTGGGAAAGAAGGATGATAGCAGAGAGGAGAGGATTGATGAGTCTGCAATTTCAAGGCCGTACCTTTCTGAAGCTTGGGAGGTTAGGGAGAAGAGGAAGAGAGAGAAGCCATTGATGAATTGGAGGTTTCCTGTGTTGGAAAATGAAATTGACATGAAAGACAATCTCAAATGGTGGGCTCATACTGTTGCTTCTGCAGTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

200

Amino Acids

23.35

Weight (kDa)

9.57

Isoelectric Point (pI)

62.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 95 - 129 4.7e-06 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 170
AclWI GGATC 1 cut(s) 330
AcuI CTGAAG 2 cut(s) 278, 483
AfaI GTAC 1 cut(s) 455
AgsI TTSAA 6 cut(s) 109, 242, 302, 360, 372, 447
AjnI CCWGG 4 cut(s) 59, 68, 182, 379
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AluBI AGCT 6 cut(s) 93, 105, 154, 282, 307, 467
AluI AGCT 6 cut(s) 93, 105, 154, 282, 307, 467
Alw21I GWGCWC 2 cut(s) 95, 119
AlwI GGATC 1 cut(s) 330
Aor13HI TCCGGA 1 cut(s) 170
AoxI GGCC 1 cut(s) 449
ApeKI GCWGC 1 cut(s) 385
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
AspS9I GGNCC 2 cut(s) 72, 289
AvaII GGWCC 2 cut(s) 72, 289
BanII GRGCYC 2 cut(s) 95, 574
Bbv12I GWGCWC 2 cut(s) 95, 119
BbvI GCAGC 1 cut(s) 372
BccI CCATC 1 cut(s) 37
BceAI ACGGC 1 cut(s) 436
BciT130I CCWGG 4 cut(s) 61, 70, 184, 381
BfmI CTRYAG 1 cut(s) 588
BisI GCNGC 1 cut(s) 386
BlsI GCNGC 1 cut(s) 387
Bme1390I CCNGG 4 cut(s) 61, 70, 184, 381
Bme18I GGWCC 2 cut(s) 72, 289
BmgT120I GGNCC 2 cut(s) 72, 289
BmrFI CCNGG 4 cut(s) 61, 70, 184, 381
BplI GAGNNNNNCTC 2 cut(s) 77, 109
BpuEI CTTGAG 1 cut(s) 133
BsaJI CCNNGG 2 cut(s) 183, 380
BsaWI WCCGGW 1 cut(s) 170
BseAI TCCGGA 1 cut(s) 170
BseBI CCWGG 4 cut(s) 61, 70, 184, 381
BseDI CCNNGG 2 cut(s) 183, 380
BseGI GGATG 1 cut(s) 412
BseMII CTCAG 1 cut(s) 354
BseRI GAGGAG 3 cut(s) 262, 265, 434
BseXI GCAGC 1 cut(s) 372
BshFI GGCC 1 cut(s) 451
BsiHKAI GWGCWC 2 cut(s) 95, 119
BsiSI CCGG 2 cut(s) 128, 171
BsnI GGCC 1 cut(s) 451
Bsp1286I GDGCHC 3 cut(s) 95, 119, 574
Bsp13I TCCGGA 1 cut(s) 170
Bsp143I GATC 1 cut(s) 322
BspANI GGCC 1 cut(s) 451
BspCNI CTCAG 1 cut(s) 353
BspEI TCCGGA 1 cut(s) 170
BspMAI CTGCAG 1 cut(s) 592
BspPI GGATC 1 cut(s) 330
BspQI GCTCTTC 1 cut(s) 297
BssECI CCNNGG 2 cut(s) 183, 380
BssMI GATC 1 cut(s) 322
Bst2UI CCWGG 4 cut(s) 61, 70, 184, 381
Bst4CI ACNGT 1 cut(s) 580
Bst6I CTCTTC 4 cut(s) 260, 297, 479, 485
BstDEI CTNAG 1 cut(s) 340
BstF5I GGATG 1 cut(s) 412
BstKTI GATC 1 cut(s) 325
BstMBI GATC 1 cut(s) 322
BstNI CCWGG 4 cut(s) 61, 70, 184, 381
BstSCI CCNGG 4 cut(s) 59, 68, 182, 379
BstSFI CTRYAG 1 cut(s) 588
BstV1I GCAGC 1 cut(s) 372
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BsuRI GGCC 1 cut(s) 451
BtsCI GGATG 1 cut(s) 412
Cfr13I GGNCC 2 cut(s) 72, 289
CsiI ACCWGGT 1 cut(s) 68
Csp6I GTAC 1 cut(s) 454
CviAII CATG 2 cut(s) 76, 547
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 1 cut(s) 340
DpnI GATC 1 cut(s) 324
DpnII GATC 1 cut(s) 322
Eam1104I CTCTTC 4 cut(s) 260, 297, 479, 485
EarI CTCTTC 4 cut(s) 260, 297, 479, 485
Ecl136II GAGCTC 1 cut(s) 93
Eco24I GRGCYC 2 cut(s) 95, 574
Eco47I GGWCC 2 cut(s) 72, 289
Eco53kI GAGCTC 1 cut(s) 93
Eco57I CTGAAG 2 cut(s) 278, 483
EcoICRI GAGCTC 1 cut(s) 93
EcoRII CCWGG 4 cut(s) 59, 68, 182, 379
EcoT38I GRGCYC 2 cut(s) 95, 574
FaeI CATG 2 cut(s) 79, 550
FaiI YATR 5 cut(s) 77, 211, 320, 548, 576
FatI CATG 2 cut(s) 75, 546
Fnu4HI GCNGC 1 cut(s) 386
FokI GGATG 1 cut(s) 419
FriOI GRGCYC 2 cut(s) 95, 574
Fsp4HI GCNGC 1 cut(s) 386
GluI GCNGC 1 cut(s) 386
HaeIII GGCC 1 cut(s) 451
HapII CCGG 2 cut(s) 128, 171
Hin1II CATG 2 cut(s) 79, 550
HindIII AAGCTT 1 cut(s) 465
HinfI GANTC 4 cut(s) 52, 131, 356, 434
HpaII CCGG 2 cut(s) 128, 171
Hpy188I TCNGA 4 cut(s) 194, 217, 343, 463
Hpy188III TCNNGA 1 cut(s) 171
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 2 cut(s) 302, 397
HpyCH4III ACNGT 1 cut(s) 580
HpyCH4V TGCA 3 cut(s) 388, 440, 590
HpyF3I CTNAG 1 cut(s) 340
Hsp92II CATG 2 cut(s) 79, 550
Kpn2I TCCGGA 1 cut(s) 170
Kzo9I GATC 1 cut(s) 322
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 1 cut(s) 98
Lsp1109I GCAGC 1 cut(s) 372
MabI ACCWGGT 1 cut(s) 68
MalI GATC 1 cut(s) 324
MboI GATC 1 cut(s) 322
MflI RGATCY 1 cut(s) 322
MhlI GDGCHC 3 cut(s) 95, 119, 574
MluCI AATT 4 cut(s) 162, 441, 511, 540
MlyI GAGTC 2 cut(s) 125, 443
MmeI TCCRAC 2 cut(s) 80, 510
MnlI CCTC 9 cut(s) 149, 240, 243, 337, 412, 417, 466, 480, 510
MroI TCCGGA 1 cut(s) 170
MspI CCGG 2 cut(s) 128, 171
MspR9I CCNGG 4 cut(s) 61, 70, 184, 381
MvaI CCWGG 4 cut(s) 61, 70, 184, 381
NdeII GATC 1 cut(s) 322
NlaIII CATG 2 cut(s) 79, 550
PciSI GCTCTTC 1 cut(s) 297
PfeI GAWTC 2 cut(s) 52, 356
PflFI GACNNNGTC 1 cut(s) 134
PkrI GCNGC 1 cut(s) 387
PleI GAGTC 2 cut(s) 125, 442
PpsI GAGTC 2 cut(s) 125, 442
Psp124BI GAGCTC 1 cut(s) 95
Psp6I CCWGG 4 cut(s) 59, 68, 182, 379
PspGI CCWGG 4 cut(s) 59, 68, 182, 379
PspPI GGNCC 2 cut(s) 72, 289
PstI CTGCAG 1 cut(s) 592
PsuI RGATCY 1 cut(s) 322
PsyI GACNNNGTC 1 cut(s) 134
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
SacI GAGCTC 1 cut(s) 95
SapI GCTCTTC 1 cut(s) 297
SatI GCNGC 1 cut(s) 386
Sau3AI GATC 1 cut(s) 322
Sau96I GGNCC 2 cut(s) 72, 289
SchI GAGTC 2 cut(s) 125, 443
ScrFI CCNGG 4 cut(s) 61, 70, 184, 381
SduI GDGCHC 3 cut(s) 95, 119, 574
SexAI ACCWGGT 1 cut(s) 68
SfcI CTRYAG 1 cut(s) 588
SinI GGWCC 2 cut(s) 72, 289
SmlI CTYRAG 1 cut(s) 112
SmoI CTYRAG 1 cut(s) 112
Sse9I AATT 4 cut(s) 162, 441, 511, 540
SstI GAGCTC 1 cut(s) 95
StyD4I CCNGG 4 cut(s) 59, 68, 182, 379
TaaI ACNGT 1 cut(s) 580
TasI AATT 4 cut(s) 162, 441, 511, 540
TfiI GAWTC 2 cut(s) 52, 356
TseI GCWGC 1 cut(s) 385
TspDTI ATGAA 3 cut(s) 524, 552, 563
TspGWI ACGGA 3 cut(s) 24, 64, 302
Tth111I GACNNNGTC 1 cut(s) 134
VpaK11BI GGWCC 2 cut(s) 72, 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.