pycom05g27480

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003875
Physical Location & Seq
Forward (+)
28478490 .. 28479011
522 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g27480.1

Sequence Viewer

Length: 522 bp
ATGAGCGACGAGTTGAGCTCCAAAACGACCTTCAACTTGAACTCTCTCTCGCCGGACTCTGTCCTCGACGGACCGAAGCTCTCAACTGTTCTTTCCGGAAAAGAAGCCGGGGAAATCGAAAACCAGAAACAGGTATGTGCAGAAGAAGAAACACCGAAGAAAGTTGAAAGGAGGAGAAGAAAGAAGAAGAGTGAGAGCAAGAGCTTGACGGACCTTCAGTTTGAAGAGCTAAAAGGGTTTATGGATCTTGGTTTTGTTTTCTCAGAGGAAGATAAAGATTCGAACTTGGTTTCAATCATTCCTGGGCTGCAAAGATTGGGAAAGAAGGATGATAGCAGATATGAGAGTTTTGATGAGTCTGCAATTTCAAGGCCGTACCTTTCTGAAGCTTGGAAGGTTAGGGAGAAGAGGAAGAGAGGGAAGCCATTGATGAATTGGAGGTTTCCCGCTTTGGAGAATGAAATTGACATGAAAGACAATCTCAAATGGTGGGCTCATACTGTTGCTTCTGCAGTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

174

Amino Acids

19.9

Weight (kDa)

7.75

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 69 - 103 3.7e-06 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 95
AciI CCGC 1 cut(s) 447
AclWI GGATC 1 cut(s) 252
AcuI CTGAAG 2 cut(s) 200, 405
AfaI GTAC 1 cut(s) 377
AfiI CCNNNNNNNGG 1 cut(s) 130
AgsI TTSAA 6 cut(s) 34, 40, 167, 224, 294, 369
AjnI CCWGG 1 cut(s) 301
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AluBI AGCT 5 cut(s) 18, 79, 204, 229, 389
AluI AGCT 5 cut(s) 18, 79, 204, 229, 389
Alw21I GWGCWC 1 cut(s) 20
AlwI GGATC 1 cut(s) 252
Aor13HI TCCGGA 1 cut(s) 95
AoxI GGCC 1 cut(s) 371
ApeKI GCWGC 1 cut(s) 307
ArsI GACNNNNNNTTYG 2 cut(s) 203, 235
AspS9I GGNCC 2 cut(s) 71, 211
AsuC2I CCSGG 1 cut(s) 109
AsuII TTCGAA 1 cut(s) 281
AvaII GGWCC 2 cut(s) 71, 211
BanII GRGCYC 2 cut(s) 20, 496
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 1 cut(s) 294
BceAI ACGGC 1 cut(s) 358
BciT130I CCWGG 1 cut(s) 303
BcnI CCSGG 1 cut(s) 109
BfmI CTRYAG 1 cut(s) 510
BisI GCNGC 1 cut(s) 308
BlsI GCNGC 1 cut(s) 309
Bme1390I CCNGG 2 cut(s) 109, 303
Bme18I GGWCC 2 cut(s) 71, 211
BmgT120I GGNCC 2 cut(s) 71, 211
BmrFI CCNGG 2 cut(s) 109, 303
BplI GAGNNNNNCTC 1 cut(s) 34
Bpu14I TTCGAA 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 108, 302
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 130
BseAI TCCGGA 1 cut(s) 95
BseBI CCWGG 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 108, 302
BseGI GGATG 1 cut(s) 334
BseLI CCNNNNNNNGG 1 cut(s) 130
BseMII CTCAG 1 cut(s) 276
BseRI GAGGAG 1 cut(s) 187
BseXI GCAGC 1 cut(s) 294
BsgI GTGCAG 1 cut(s) 159
BshFI GGCC 1 cut(s) 373
BsiHKAI GWGCWC 1 cut(s) 20
BsiSI CCGG 3 cut(s) 53, 96, 108
BslI CCNNNNNNNGG 1 cut(s) 130
BsnI GGCC 1 cut(s) 373
Bsp119I TTCGAA 1 cut(s) 281
Bsp1286I GDGCHC 2 cut(s) 20, 496
Bsp13I TCCGGA 1 cut(s) 95
Bsp143I GATC 1 cut(s) 244
BspACI CCGC 1 cut(s) 447
BspANI GGCC 1 cut(s) 373
BspCNI CTCAG 1 cut(s) 275
BspEI TCCGGA 1 cut(s) 95
BspMAI CTGCAG 1 cut(s) 514
BspPI GGATC 1 cut(s) 252
BspQI GCTCTTC 1 cut(s) 219
BspT104I TTCGAA 1 cut(s) 281
BssECI CCNNGG 2 cut(s) 108, 302
BssMI GATC 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 303
Bst4CI ACNGT 2 cut(s) 88, 502
Bst6I CTCTTC 4 cut(s) 182, 219, 401, 407
BstBI TTCGAA 1 cut(s) 281
BstDEI CTNAG 1 cut(s) 262
BstF5I GGATG 1 cut(s) 334
BstKTI GATC 1 cut(s) 247
BstMBI GATC 1 cut(s) 244
BstNI CCWGG 1 cut(s) 303
BstSCI CCNGG 2 cut(s) 107, 301
BstSFI CTRYAG 1 cut(s) 510
BstV1I GCAGC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
BsuRI GGCC 1 cut(s) 373
BtsCI GGATG 1 cut(s) 334
Cfr13I GGNCC 2 cut(s) 71, 211
CpoI CGGWCCG 1 cut(s) 71
Csp6I GTAC 1 cut(s) 376
CspI CGGWCCG 1 cut(s) 71
CviAII CATG 1 cut(s) 469
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 1 cut(s) 262
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
Eam1104I CTCTTC 4 cut(s) 182, 219, 401, 407
EarI CTCTTC 4 cut(s) 182, 219, 401, 407
Ecl136II GAGCTC 1 cut(s) 18
Eco24I GRGCYC 2 cut(s) 20, 496
Eco47I GGWCC 2 cut(s) 71, 211
Eco53kI GAGCTC 1 cut(s) 18
Eco57I CTGAAG 2 cut(s) 200, 405
EcoICRI GAGCTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 301
EcoT38I GRGCYC 2 cut(s) 20, 496
FaeI CATG 1 cut(s) 472
FaiI YATR 5 cut(s) 136, 242, 342, 470, 498
FatI CATG 1 cut(s) 468
FauI CCCGC 1 cut(s) 454
Fnu4HI GCNGC 1 cut(s) 308
FokI GGATG 1 cut(s) 341
FriOI GRGCYC 2 cut(s) 20, 496
Fsp4HI GCNGC 1 cut(s) 308
GluI GCNGC 1 cut(s) 308
HaeIII GGCC 1 cut(s) 373
HapII CCGG 3 cut(s) 53, 96, 108
Hin1II CATG 1 cut(s) 472
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 3 cut(s) 56, 278, 356
HpaII CCGG 3 cut(s) 53, 96, 108
Hpy188I TCNGA 2 cut(s) 265, 385
Hpy188III TCNNGA 1 cut(s) 96
Hpy99I CGWCG 2 cut(s) 11, 71
HpyAV CCTTC 4 cut(s) 40, 224, 319, 388
HpyCH4III ACNGT 2 cut(s) 88, 502
HpyCH4V TGCA 4 cut(s) 140, 310, 362, 512
HpyF3I CTNAG 1 cut(s) 262
Hsp92II CATG 1 cut(s) 472
Kpn2I TCCGGA 1 cut(s) 95
Kzo9I GATC 1 cut(s) 244
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 7 cut(s) 66, 109, 116, 121, 137, 288, 315
Lsp1109I GCAGC 1 cut(s) 294
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MflI RGATCY 1 cut(s) 244
MhlI GDGCHC 2 cut(s) 20, 496
MluCI AATT 3 cut(s) 363, 433, 462
MlyI GAGTC 2 cut(s) 50, 365
MnlI CCTC 6 cut(s) 74, 165, 259, 402, 410, 432
MroI TCCGGA 1 cut(s) 95
MspI CCGG 3 cut(s) 53, 96, 108
MspR9I CCNGG 2 cut(s) 109, 303
MvaI CCWGG 1 cut(s) 303
NciI CCSGG 1 cut(s) 109
NdeII GATC 1 cut(s) 244
NlaIII CATG 1 cut(s) 472
NspV TTCGAA 1 cut(s) 281
PciSI GCTCTTC 1 cut(s) 219
PfeI GAWTC 1 cut(s) 278
PflFI GACNNNGTC 1 cut(s) 59
PkrI GCNGC 1 cut(s) 309
PleI GAGTC 2 cut(s) 50, 364
PpsI GAGTC 2 cut(s) 50, 364
Psp124BI GAGCTC 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 301
PspGI CCWGG 1 cut(s) 301
PspPI GGNCC 2 cut(s) 71, 211
PstI CTGCAG 1 cut(s) 514
PsuI RGATCY 1 cut(s) 244
PsyI GACNNNGTC 1 cut(s) 59
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
Rsr2I CGGWCCG 1 cut(s) 71
RsrII CGGWCCG 1 cut(s) 71
SacI GAGCTC 1 cut(s) 20
SapI GCTCTTC 1 cut(s) 219
SatI GCNGC 1 cut(s) 308
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 2 cut(s) 71, 211
SchI GAGTC 2 cut(s) 50, 365
ScrFI CCNGG 2 cut(s) 109, 303
SduI GDGCHC 2 cut(s) 20, 496
SfcI CTRYAG 1 cut(s) 510
SfuI TTCGAA 1 cut(s) 281
SinI GGWCC 2 cut(s) 71, 211
Sse9I AATT 3 cut(s) 363, 433, 462
SsiI CCGC 1 cut(s) 447
SstI GAGCTC 1 cut(s) 20
StyD4I CCNGG 2 cut(s) 107, 301
TaaI ACNGT 2 cut(s) 88, 502
TaqI TCGA 3 cut(s) 66, 117, 281
TaqII GACCGA 1 cut(s) 88
TasI AATT 3 cut(s) 363, 433, 462
TfiI GAWTC 1 cut(s) 278
TseI GCWGC 1 cut(s) 307
TspDTI ATGAA 3 cut(s) 446, 474, 485
TspGWI ACGGA 2 cut(s) 84, 224
Tth111I GACNNNGTC 1 cut(s) 59
VpaK11BI GGWCC 2 cut(s) 71, 211
XcmI CCANNNNNNNNNTGG 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.