MD10G1276400.v1.1

Protein of unknown function (DUF1685)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003875
Physical Location & Seq
Forward (+)
36744204 .. 36744917
714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1276400.v1.1.491

Sequence Viewer

Length: 714 bp
ATGGAAGCAGACGAAGTAATGGATTTATACGATTCTTTCTGGTTCAAGTTCCCGATCTTCAAGAAACAACCAGTTTCATCGAACCCATCAAATTTTGAAGAACACCCAGATCATGAAATCGAAGAAAACCCAGAAAAACCATCAAAGCCAGAGTTTTTACGGATTCCAACCAGGCACACCAGGTCCATGAGCGACGAGTTGAGCTCCAATACGAGCTTCAACTTGAGCACTCTCTCGCCGGACACTGTCCTCCACAGACCGAAGCTCTCCACAATTCTTTCCGGAAAAGAAGCCAGGGAAATCGGAAACCCGAAACAGATATATTCAGAAGAAGAACCACAGAAGAAAGTTGAAAGGAGGAGGAGGAGAAGAAAGAAGAAGAGTGAGAGCAAGAGCTTGACAGACCTTCAGTTTGAAGAGCTAAAAGGGTTTATGGATCTTGGTTTTGTTTTCTCAGAGGAAGATAAAGATTCAAACTTGGTTTCAATCATTCCTGGGCTGCAAAGATTGGGAAAGAAGGATGACAGCAGAGAGGAGAGGTTTAATGAGTCTGCAATTTCAAGGCCGTACCTTTCTGAAGCTTGGGAGGTTAGGGAGAAGAGGAAGAGAGAGAAGCCATTGATGAATTGGAGGTTTCCTGCGTTGGAAAATGAAATTGACATGAAAGACAATCTCAAATGGTGGGCTCATACTGTTGCTTCTGCAGTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1685

Protein Analysis

238

Amino Acids

27.99

Weight (kDa)

6.57

Isoelectric Point (pI)

62.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 133 - 167 6.1e-06 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 281
AclWI GGATC 1 cut(s) 444
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 2 cut(s) 392, 597
AfaI GTAC 1 cut(s) 569
AgsI TTSAA 9 cut(s) 46, 61, 98, 220, 353, 416, 474, 486, 561
AjnI CCWGG 4 cut(s) 170, 179, 293, 493
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 6 cut(s) 204, 216, 265, 396, 421, 581
AluI AGCT 6 cut(s) 204, 216, 265, 396, 421, 581
Alw21I GWGCWC 2 cut(s) 206, 230
AlwI GGATC 1 cut(s) 444
Aor13HI TCCGGA 1 cut(s) 281
AoxI GGCC 1 cut(s) 563
ApeKI GCWGC 1 cut(s) 499
ApoI RAATTY 1 cut(s) 91
ArsI GACNNNNNNTTYG 2 cut(s) 395, 427
AspS9I GGNCC 1 cut(s) 183
AvaII GGWCC 1 cut(s) 183
BanII GRGCYC 2 cut(s) 206, 688
Bbv12I GWGCWC 2 cut(s) 206, 230
BbvI GCAGC 1 cut(s) 486
BccI CCATC 2 cut(s) 94, 148
BceAI ACGGC 1 cut(s) 550
BciT130I CCWGG 4 cut(s) 172, 181, 295, 495
BfmI CTRYAG 1 cut(s) 702
BisI GCNGC 1 cut(s) 500
BlsI GCNGC 1 cut(s) 501
Bme1390I CCNGG 4 cut(s) 172, 181, 295, 495
Bme18I GGWCC 1 cut(s) 183
BmgT120I GGNCC 1 cut(s) 183
BmrFI CCNGG 4 cut(s) 172, 181, 295, 495
BplI GAGNNNNNCTC 2 cut(s) 188, 220
BpuEI CTTGAG 1 cut(s) 244
BsaJI CCNNGG 2 cut(s) 294, 494
BsaWI WCCGGW 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 71
BseAI TCCGGA 1 cut(s) 281
BseBI CCWGG 4 cut(s) 172, 181, 295, 495
BseDI CCNNGG 2 cut(s) 294, 494
BseGI GGATG 1 cut(s) 526
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 1 cut(s) 71
BseRI GAGGAG 4 cut(s) 373, 376, 379, 548
BseXI GCAGC 1 cut(s) 486
BshFI GGCC 1 cut(s) 565
BsiHKAI GWGCWC 2 cut(s) 206, 230
BsiSI CCGG 2 cut(s) 239, 282
BsnI GGCC 1 cut(s) 565
Bsp1286I GDGCHC 3 cut(s) 206, 230, 688
Bsp13I TCCGGA 1 cut(s) 281
Bsp143I GATC 3 cut(s) 54, 109, 436
BspANI GGCC 1 cut(s) 565
BspCNI CTCAG 1 cut(s) 467
BspEI TCCGGA 1 cut(s) 281
BspHI TCATGA 1 cut(s) 112
BspMAI CTGCAG 1 cut(s) 706
BspPI GGATC 1 cut(s) 444
BspQI GCTCTTC 1 cut(s) 411
BsrI ACTGG 1 cut(s) 71
BssECI CCNNGG 2 cut(s) 294, 494
BssMI GATC 3 cut(s) 54, 109, 436
Bst2UI CCWGG 4 cut(s) 172, 181, 295, 495
Bst4CI ACNGT 2 cut(s) 247, 694
Bst6I CTCTTC 4 cut(s) 374, 411, 593, 599
BstDEI CTNAG 1 cut(s) 454
BstF5I GGATG 1 cut(s) 526
BstKTI GATC 3 cut(s) 57, 112, 439
BstMBI GATC 3 cut(s) 54, 109, 436
BstNI CCWGG 4 cut(s) 172, 181, 295, 495
BstSCI CCNGG 4 cut(s) 170, 179, 293, 493
BstSFI CTRYAG 1 cut(s) 702
BstV1I GCAGC 1 cut(s) 486
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BsuRI GGCC 1 cut(s) 565
BtsCI GGATG 1 cut(s) 526
BtsIMutI CAGTG 1 cut(s) 243
CciI TCATGA 1 cut(s) 112
Cfr13I GGNCC 1 cut(s) 183
CsiI ACCWGGT 1 cut(s) 179
Csp6I GTAC 1 cut(s) 568
CviAII CATG 3 cut(s) 113, 187, 661
CviQI GTAC 1 cut(s) 568
DdeI CTNAG 1 cut(s) 454
DpnI GATC 3 cut(s) 56, 111, 438
DpnII GATC 3 cut(s) 54, 109, 436
Eam1104I CTCTTC 4 cut(s) 374, 411, 593, 599
EarI CTCTTC 4 cut(s) 374, 411, 593, 599
Ecl136II GAGCTC 1 cut(s) 204
Eco24I GRGCYC 2 cut(s) 206, 688
Eco47I GGWCC 1 cut(s) 183
Eco53kI GAGCTC 1 cut(s) 204
Eco57I CTGAAG 2 cut(s) 392, 597
EcoICRI GAGCTC 1 cut(s) 204
EcoRII CCWGG 4 cut(s) 170, 179, 293, 493
EcoT38I GRGCYC 2 cut(s) 206, 688
FaeI CATG 3 cut(s) 116, 190, 664
FaiI YATR 7 cut(s) 28, 114, 188, 322, 434, 662, 690
FatI CATG 3 cut(s) 112, 186, 660
Fnu4HI GCNGC 1 cut(s) 500
FokI GGATG 1 cut(s) 533
FriOI GRGCYC 2 cut(s) 206, 688
Fsp4HI GCNGC 1 cut(s) 500
GluI GCNGC 1 cut(s) 500
HaeIII GGCC 1 cut(s) 565
HapII CCGG 2 cut(s) 239, 282
Hin1II CATG 3 cut(s) 116, 190, 664
HindIII AAGCTT 1 cut(s) 579
HinfI GANTC 4 cut(s) 32, 163, 470, 548
HpaII CCGG 2 cut(s) 239, 282
Hpy188I TCNGA 4 cut(s) 305, 328, 457, 577
Hpy188III TCNNGA 4 cut(s) 52, 61, 113, 282
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 2 cut(s) 416, 511
HpyCH4III ACNGT 2 cut(s) 247, 694
HpyCH4V TGCA 3 cut(s) 502, 554, 704
HpyF3I CTNAG 1 cut(s) 454
Hsp92II CATG 3 cut(s) 116, 190, 664
Kpn2I TCCGGA 1 cut(s) 281
Kzo9I GATC 3 cut(s) 54, 109, 436
LguI GCTCTTC 1 cut(s) 411
LmnI GCTCC 1 cut(s) 209
Lsp1109I GCAGC 1 cut(s) 486
MabI ACCWGGT 1 cut(s) 179
MalI GATC 3 cut(s) 56, 111, 438
MboI GATC 3 cut(s) 54, 109, 436
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 3 cut(s) 206, 230, 688
MluCI AATT 5 cut(s) 91, 273, 555, 625, 654
MlyI GAGTC 1 cut(s) 557
MmeI TCCRAC 2 cut(s) 191, 624
MroI TCCGGA 1 cut(s) 281
MseI TTAA 1 cut(s) 543
MspI CCGG 2 cut(s) 239, 282
MspR9I CCNGG 4 cut(s) 172, 181, 295, 495
MvaI CCWGG 4 cut(s) 172, 181, 295, 495
NdeII GATC 3 cut(s) 54, 109, 436
NlaIII CATG 3 cut(s) 116, 190, 664
PagI TCATGA 1 cut(s) 112
PciSI GCTCTTC 1 cut(s) 411
PfeI GAWTC 3 cut(s) 32, 163, 470
PflFI GACNNNGTC 1 cut(s) 245
PkrI GCNGC 1 cut(s) 501
PleI GAGTC 1 cut(s) 556
PpsI GAGTC 1 cut(s) 556
Psp124BI GAGCTC 1 cut(s) 206
Psp6I CCWGG 4 cut(s) 170, 179, 293, 493
PspGI CCWGG 4 cut(s) 170, 179, 293, 493
PspPI GGNCC 1 cut(s) 183
PstI CTGCAG 1 cut(s) 706
PsuI RGATCY 1 cut(s) 436
PsyI GACNNNGTC 1 cut(s) 245
RsaI GTAC 1 cut(s) 569
RsaNI GTAC 1 cut(s) 568
SacI GAGCTC 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 411
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 1 cut(s) 500
Sau3AI GATC 3 cut(s) 54, 109, 436
Sau96I GGNCC 1 cut(s) 183
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 4 cut(s) 172, 181, 295, 495
SduI GDGCHC 3 cut(s) 206, 230, 688
SexAI ACCWGGT 1 cut(s) 179
SfcI CTRYAG 1 cut(s) 702
SinI GGWCC 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
Sse9I AATT 5 cut(s) 91, 273, 555, 625, 654
SstI GAGCTC 1 cut(s) 206
StyD4I CCNGG 4 cut(s) 170, 179, 293, 493
TaaI ACNGT 2 cut(s) 247, 694
TaqI TCGA 2 cut(s) 80, 120
TaqII GACCGA 1 cut(s) 274
TasI AATT 5 cut(s) 91, 273, 555, 625, 654
TfiI GAWTC 3 cut(s) 32, 163, 470
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TscAI CASTG 1 cut(s) 250
TseI GCWGC 1 cut(s) 499
TspDTI ATGAA 5 cut(s) 66, 129, 638, 666, 677
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 1 cut(s) 250
Tth111I GACNNNGTC 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 183
XapI RAATTY 1 cut(s) 91
XcmI CCANNNNNNNNNTGG 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.