Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003265

Physical Location & Seq

Genomic Coordinates Forward (+)

45161593 .. 45164207

Gene Structure 2615 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1325500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1890 bp

ATGGCCTCAGCGAAACGCGAAGCTCCTCCCAGTGAAGACCAACCCCAGGAGGCCCTGAAGAAGGCCAGGGTCGCCGGCGATAACGTTTCGTCCTGCAACTCGTCTTCAGAACCCCTAAACCGGAAGCAGCGTGTAGCGCTCAATCGCGCCGACTGTGCCCTAGATTTTGTAATCGAGGGCAATGAGCGTCGAGGATATGCACTTTGCGATCAGGGCTTTGCTTATTGCTGGTCCGGAGCTCGAGCGAATGTGGGGATTAATGGAGGGAAGTACTGTTTCGGCTGCAAGATCGTGTCTTATCAGTCGGTGGACATGGAGGATACTAAGCTTGATCAGCAGCATCTATGCCGACTCGGAATTTCGAGAGGGGATGATGCGGTTGGGAGCCTTGGGGAAACAGCTTTCGGCTTTGGGTATGGGGGCACTGGAAAATTCTCGTATCATAGCAAGTTTTCGGATTATGGTGAGAAGTTTGGTCTTGGCGATACCATAGCGTGTGCTGTCGATCTCGAGGATAAATCTCTTGCTTCCATTGGGTTCTATAAGAATGGGAAATGGTTGGGTCGAGCAACGTTTTTCGAGGTTAAGGTTTTGGGCGTTTCAGGGGTGGATACTGCTGCGGGGAAGATGGAATGGGAGTCGGGGTTTTTCCCTCACGTTTTGTTGAAAAATGTGGTGGTGGAGCTGCAGTTCAATGTTGAAGATGGACTTGTTGCTGAAGAGGGTTTTAAGCCTTGGGCATGTGCACTTGAGGATGGAAATGCAGTGATGGGACCTGCTTTTTCAGACCCGAGGGCTTGTGAAGTGGTTATGATGGTGGGTTTGCCTGCATCTGGAAAGACTACTTGGGCAGAGAAATGGGCGAAACAACATCCCGAGAAGCGTTATATTGTGCTTGGAACAAATTTAATTCTCGATCAAATGAAGGTGCCAGGGCTTTTGCGCGAGAGCAATTATGGTGAACGATTTCATTTCCTGATGAATCGAGCAACGGATATTTTTAACGTCCTTTTATCCAGAGCAGCCACCACACCTCGCAATTACATAATTGATCAGACAAATGTATACAAGAGTGCTCGTAAACGTAAACTTAAGCCATTTGCTTTCTTCCGGAAGATTGCTGTTGTGGTGTTCCCAAGCCCCGAAGTGCTGAGTGTTCGTTCCGAGAAAAGAATGGAAGAAATGGGAAAGGAGCTCCCTGCTGAAGCAGTAAATAATATGTTAGCCAATTTTGTTTTACCTGTCAGCAAGGATATGCGTGGTTCAGATGAGTACTTCGACGAGGCTATGTTTGTAGAACTCAACAGAGAAGAGTCACAGAGATATTTGGATGAGATGAAGCGGTCACTCGCAAGCATTGACTCTAATACGTATTCTGTTGGAAGCTCTGTTCCTACGTTCTCACCAAATTATGGGCAAAGTCTTGTGCTGCCAATTCCTCCGCCTGCACCTAGGATGACTGTGGTGCGGCCATATCCTATTGTTTCGCCTTCTCCAAGGGCTGTTAACCCTTCTGGAACTTATTCTGGCTACTCGGGCAGTTACAGTGGTGTTCCTAGGGGAGGTACAGATCATTACCAGAGTTATGGACGACCAGGTGCTCCCACTAACCCCTACGGTAGTAGTACCAGTGGAGCTTATCCAGTAGGCAGTGCAACAACTTCTTTCACAGGCAATGCCTACGGACATCTTGGTGGTGCTGGAGATCCCAGAAGTGCTGCTCCCTGGGCTCCAATAGCACCCACATTTCTTCCCAATCCTTCCCCACCTGCACATGGAGGCTTACCATATGGAACTCCAGCTCCTGGTCCTCAGTACGGACATCTTCCTCCTGTTAACACTCCATATCATGGAGGCCATTACCCTCCTACGCCGGGGTACTACCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0019899 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0070013

KEGG Pathways

Metabolic & Signaling

ko05164 map05164

Pfam Domains

Protein Families

AAA_33 SPRY

Protein Analysis

630

Amino Acids

68.34

Weight (kDa)

7.55

Isoelectric Point (pI)

40.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPRY	PF00622	111 - 190	2.7e-08	SPRY domain
AAA_33	PF13671	269 - 414	9.4e-25	AAA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003265)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g03980 FvH4_3g03980 FvH4_3g03980 FvH4_3g03980 FvH4_6g02023
malus_domestica	MD05G1325500.v1.1
prunus_persica	Prupe.4G036900_v2.0.a1
pyrus_communis	pycom05g29950 pycom10g25600
rosa_chinensis	RchiOBHm_Chr5g0006311 RchiOBHm_Chr7g0240451
rosa_laevigata	RLG00000031365
rosa_multiflora	Rmu_co8212078.1_g000001 Rmu_co8265987.1_g000001 Rmu_sc0003884.1_g000002 Rmu_sc0005327.1_g000018
rosa_roxburghii	Rroxscaffold_1G00069680 Rroxscaffold_3G00220000 Rroxscaffold_3G00220020 Rroxscaffold_3G00220060
rosa_rugosa	Rorug04G0421000 Rorug07G0335900 Rorug07G0336000 Rorug07G0336100 Rorug07G0336200
rosa_samantha	Rh5AG048900 Rh5BG048000 Rh5BG048100 Rh5CG056300 Rh5DG047200 Rh7AG490300 Rh7AG490400 Rh7BG463200 Rh7CG506700 Rh7DG474300
rosa_wichuraiana	Rw5G004870 Rw7G041090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1777
Acc36I	ACCTGC	2 cut(s)	784, 1777
AccB1I	GGYRCC	1 cut(s)	928
AccB7I	CCANNNNNTGG	3 cut(s)	1412, 1805, 1850
AccI	GTMKAC	1 cut(s)	1065
AccII	CGCG	3 cut(s)	18, 147, 945
AccIII	TCCGGA	2 cut(s)	233, 1110
AciI	CCGC	5 cut(s)	377, 620, 1342, 1442, 1468
AclI	AACGTT	2 cut(s)	84, 572
AclWI	GGATC	1 cut(s)	1700
AcoI	YGGCCR	1 cut(s)	1469
AcsI	RAATTY	3 cut(s)	357, 431, 904
AcuI	CTGAAG	4 cut(s)	77, 90, 738, 1224
AfaI	GTAC	6 cut(s)	272, 1274, 1567, 1627, 1817, 1880
AfeI	AGCGCT	1 cut(s)	138
AflII	CTTAAG	1 cut(s)	1091
AgsI	TTSAA	3 cut(s)	667, 694, 701
AjnI	CCWGG	6 cut(s)	45, 65, 931, 1594, 1724, 1804
AjuI	GAANNNNNNNTTGG	2 cut(s)	829, 861
AloI	GAACNNNNNNTCC	6 cut(s)	674, 706, 1374, 1406, 1786, 1818
AluBI	AGCT	9 cut(s)	23, 239, 328, 401, 685, 1195, 1386, 1637, 1802
AluI	AGCT	9 cut(s)	23, 239, 328, 401, 685, 1195, 1386, 1637, 1802
Alw21I	GWGCWC	5 cut(s)	241, 748, 1078, 1197, 1603
Alw44I	GTGCAC	1 cut(s)	744
AlwI	GGATC	1 cut(s)	1700
AlwNI	CAGNNNCTG	1 cut(s)	1805
Ama87I	CYCGRG	5 cut(s)	240, 509, 790, 875, 1534
Aor13HI	TCCGGA	2 cut(s)	233, 1110
Aor51HI	AGCGCT	1 cut(s)	138
AoxI	GGCC	5 cut(s)	3, 51, 63, 1469, 1855
ApaLI	GTGCAC	1 cut(s)	744
ApeKI	GCWGC	8 cut(s)	127, 282, 337, 617, 685, 1022, 1429, 1718
ApoI	RAATTY	3 cut(s)	357, 431, 904
AseI	ATTAAT	1 cut(s)	258
Asp700I	GAANNNNTTC	2 cut(s)	966, 1522
AspA2I	CCTAGG	2 cut(s)	1451, 1556
AspLEI	GCGC	3 cut(s)	139, 149, 945
AspS9I	GGNCC	4 cut(s)	52, 231, 773, 1808
AsuC2I	CCSGG	1 cut(s)	1875
AsuHPI	GGTGA	3 cut(s)	476, 971, 1395
AvaI	CYCGRG	5 cut(s)	240, 509, 790, 875, 1534
AvaII	GGWCC	3 cut(s)	231, 773, 1808
AvrII	CCTAGG	2 cut(s)	1451, 1556
BaeGI	GKGCMC	3 cut(s)	160, 425, 748
BanI	GGYRCC	1 cut(s)	928
BanII	GRGCYC	3 cut(s)	241, 1197, 1732
BbsI	GAAGAC	2 cut(s)	42, 96
Bbv12I	GWGCWC	5 cut(s)	241, 748, 1078, 1197, 1603
BbvCI	CCTCAGC	1 cut(s)	7
BbvI	GCAGC	8 cut(s)	139, 269, 349, 604, 672, 1034, 1416, 1705
BccI	CCATC	5 cut(s)	622, 698, 749, 763, 808
BciT130I	CCWGG	6 cut(s)	47, 67, 933, 1596, 1726, 1806
BciVI	GTATCC	2 cut(s)	313, 604
BclI	TGATCA	2 cut(s)	331, 1051
BcnI	CCSGG	1 cut(s)	1875
BfaI	CTAG	3 cut(s)	161, 1452, 1557
BfmI	CTRYAG	1 cut(s)	686
BfoI	RGCGCY	1 cut(s)	140
BfrI	CTTAAG	1 cut(s)	1091
BfuAI	ACCTGC	2 cut(s)	784, 1777
BfuI	GTATCC	2 cut(s)	313, 604
BisI	GCNGC	9 cut(s)	128, 283, 338, 618, 686, 1023, 1430, 1469, 1719
BlnI	CCTAGG	2 cut(s)	1451, 1556
BlsI	GCNGC	9 cut(s)	129, 284, 339, 619, 687, 1024, 1431, 1470, 1720
BmcAI	AGTACT	2 cut(s)	272, 1274
Bme1390I	CCNGG	7 cut(s)	47, 67, 933, 1596, 1726, 1806, 1875
Bme18I	GGWCC	3 cut(s)	231, 773, 1808
BmeT110I	CYCGRG	5 cut(s)	240, 509, 790, 875, 1534
BmgT120I	GGNCC	4 cut(s)	52, 231, 773, 1808
BmiI	GGNNCC	4 cut(s)	386, 774, 930, 1731
BmrFI	CCNGG	7 cut(s)	47, 67, 933, 1596, 1726, 1806, 1875
BmrI	ACTGGG	1 cut(s)	24
BmsI	GCATC	3 cut(s)	349, 364, 839
BmuI	ACTGGG	1 cut(s)	24
BpiI	GAAGAC	2 cut(s)	42, 96
BpmI	CTGGAG	2 cut(s)	1722, 1782
Bpu10I	CCTNAGC	1 cut(s)	7
BpuEI	CTTGAG	1 cut(s)	770
BpuMI	CCSGG	1 cut(s)	1875
BsaAI	YACGTR	1 cut(s)	1371
BsaWI	WCCGGW	3 cut(s)	120, 233, 1110
BsaXI	ACNNNNNCTCC	6 cut(s)	629, 659, 674, 704, 1786, 1816
Bse118I	RCCGGY	1 cut(s)	74
Bse1I	ACTGG	4 cut(s)	30, 430, 1629, 1643
Bse3DI	GCAATG	2 cut(s)	187, 1681
BseAI	TCCGGA	2 cut(s)	233, 1110
BseBI	CCWGG	6 cut(s)	47, 67, 933, 1596, 1726, 1806
BseGI	GGATG	5 cut(s)	376, 760, 871, 1336, 1461
BseMI	GCAATG	2 cut(s)	187, 1681
BseMII	CTCAG	3 cut(s)	21, 1142, 1826
BseNI	ACTGG	4 cut(s)	30, 430, 1629, 1643
BseRI	GAGGAG	1 cut(s)	15
BseSI	GKGCMC	3 cut(s)	160, 425, 748
BseXI	GCAGC	8 cut(s)	139, 269, 349, 604, 672, 1034, 1416, 1705
BsgI	GTGCAG	2 cut(s)	1431, 1755
Bsh1236I	CGCG	3 cut(s)	18, 147, 945
BshFI	GGCC	5 cut(s)	5, 53, 65, 1471, 1857
BshNI	GGYRCC	1 cut(s)	928
BsiHKAI	GWGCWC	5 cut(s)	241, 748, 1078, 1197, 1603
BsiHKCI	CYCGRG	5 cut(s)	240, 509, 790, 875, 1534
BsiSI	CCGG	5 cut(s)	75, 121, 234, 1111, 1874
BslFI	GGGAC	1 cut(s)	786
BsmFI	GGGAC	1 cut(s)	786
BsnI	GGCC	5 cut(s)	5, 53, 65, 1471, 1857
BsoBI	CYCGRG	5 cut(s)	240, 509, 790, 875, 1534
Bsp1286I	GDGCHC	8 cut(s)	160, 241, 425, 748, 1078, 1197, 1603, 1732
Bsp13I	TCCGGA	2 cut(s)	233, 1110
Bsp143I	GATC	8 cut(s)	208, 288, 331, 505, 916, 1051, 1570, 1705
BspACI	CCGC	5 cut(s)	377, 620, 1342, 1442, 1468
BspANI	GGCC	5 cut(s)	5, 53, 65, 1471, 1857
BspCNI	CTCAG	3 cut(s)	20, 1143, 1825
BspEI	TCCGGA	2 cut(s)	233, 1110
BspFNI	CGCG	3 cut(s)	18, 147, 945
BspLI	GGNNCC	4 cut(s)	386, 774, 930, 1731
BspMAI	CTGCAG	1 cut(s)	690
BspMI	ACCTGC	2 cut(s)	784, 1777
BspPI	GGATC	1 cut(s)	1700
BspT107I	GGYRCC	1 cut(s)	928
BspTI	CTTAAG	1 cut(s)	1091
BsrDI	GCAATG	2 cut(s)	187, 1681
BsrFI	RCCGGY	1 cut(s)	74
BsrI	ACTGG	4 cut(s)	30, 430, 1629, 1643
BssAI	RCCGGY	1 cut(s)	74
BssMI	GATC	8 cut(s)	208, 288, 331, 505, 916, 1051, 1570, 1705
BssNAI	GTATAC	1 cut(s)	1066
BssT1I	CCWWGG	5 cut(s)	388, 734, 1451, 1496, 1556
Bst1107I	GTATAC	1 cut(s)	1066
Bst2UI	CCWGG	6 cut(s)	47, 67, 933, 1596, 1726, 1806
Bst4CI	ACNGT	5 cut(s)	155, 275, 1462, 1547, 1619
Bst6I	CTCTTC	2 cut(s)	714, 1305
BstAFI	CTTAAG	1 cut(s)	1091
BstBAI	YACGTR	1 cut(s)	1371
BstC8I	GCNNGC	4 cut(s)	76, 828, 1354, 1446
BstDEI	CTNAG	4 cut(s)	7, 324, 1151, 1812
BstENI	CCTNNNNNAGG	2 cut(s)	59, 1560
BstF5I	GGATG	5 cut(s)	376, 760, 871, 1336, 1461
BstFNI	CGCG	3 cut(s)	18, 147, 945
BstH2I	RGCGCY	1 cut(s)	140
BstHHI	GCGC	3 cut(s)	139, 149, 945
BstKTI	GATC	8 cut(s)	211, 291, 334, 508, 919, 1054, 1573, 1708
BstMBI	GATC	8 cut(s)	208, 288, 331, 505, 916, 1051, 1570, 1705
BstMWI	GCNNNNNNNGC	8 cut(s)	71, 136, 155, 213, 334, 1536, 1727, 1736
BstNI	CCWGG	6 cut(s)	47, 67, 933, 1596, 1726, 1806
BstNSI	RCATGY	1 cut(s)	744
BstSCI	CCNGG	7 cut(s)	45, 65, 931, 1594, 1724, 1804, 1873
BstSFI	CTRYAG	1 cut(s)	686
BstSLI	GKGCMC	3 cut(s)	160, 425, 748
BstSNI	TACGTA	1 cut(s)	1371
BstUI	CGCG	3 cut(s)	18, 147, 945
BstV1I	GCAGC	8 cut(s)	139, 269, 349, 604, 672, 1034, 1416, 1705
BstV2I	GAAGAC	2 cut(s)	42, 96
BstX2I	RGATCY	1 cut(s)	1705
BstXI	CCANNNNNNTGG	1 cut(s)	1586
BstYI	RGATCY	1 cut(s)	1705
BstZ17I	GTATAC	1 cut(s)	1066
BsuI	GTATCC	2 cut(s)	313, 604
BsuRI	GGCC	5 cut(s)	5, 53, 65, 1471, 1857
BtsCI	GGATG	5 cut(s)	376, 760, 871, 1336, 1461
BtsI	GCAGTG	2 cut(s)	771, 1657
BtsIMutI	CAGTG	7 cut(s)	37, 423, 771, 1552, 1636, 1657, 1885
BveI	ACCTGC	2 cut(s)	784, 1777
Cac8I	GCNNGC	4 cut(s)	76, 828, 1354, 1446
CaiI	CAGNNNCTG	1 cut(s)	1805
CfoI	GCGC	3 cut(s)	139, 149, 945
Cfr10I	RCCGGY	1 cut(s)	74
Cfr13I	GGNCC	4 cut(s)	52, 231, 773, 1808
CseI	GACGC	1 cut(s)	176
CsiI	ACCWGGT	1 cut(s)	1594
Csp6I	GTAC	6 cut(s)	271, 1273, 1566, 1626, 1816, 1879
CviAII	CATG	4 cut(s)	313, 741, 1775, 1850
CviQI	GTAC	6 cut(s)	271, 1273, 1566, 1626, 1816, 1879
DdeI	CTNAG	4 cut(s)	7, 324, 1151, 1812
DpnI	GATC	8 cut(s)	210, 290, 333, 507, 918, 1053, 1572, 1707
DpnII	GATC	8 cut(s)	208, 288, 331, 505, 916, 1051, 1570, 1705
EaeI	YGGCCR	1 cut(s)	1469
Eam1104I	CTCTTC	2 cut(s)	714, 1305
EarI	CTCTTC	2 cut(s)	714, 1305
EciI	GGCGGA	1 cut(s)	1431
Ecl136II	GAGCTC	2 cut(s)	239, 1195
Eco105I	TACGTA	1 cut(s)	1371
Eco130I	CCWWGG	5 cut(s)	388, 734, 1451, 1496, 1556
Eco24I	GRGCYC	3 cut(s)	241, 1197, 1732
Eco47I	GGWCC	3 cut(s)	231, 773, 1808
Eco47III	AGCGCT	1 cut(s)	138
Eco53kI	GAGCTC	2 cut(s)	239, 1195
Eco57I	CTGAAG	4 cut(s)	77, 90, 738, 1224
Eco88I	CYCGRG	5 cut(s)	240, 509, 790, 875, 1534
EcoICRI	GAGCTC	2 cut(s)	239, 1195
EcoNI	CCTNNNNNAGG	2 cut(s)	59, 1560
EcoO109I	RGGNCCY	2 cut(s)	52, 773
EcoRII	CCWGG	6 cut(s)	45, 65, 931, 1594, 1724, 1804
EcoT14I	CCWWGG	5 cut(s)	388, 734, 1451, 1496, 1556
EcoT38I	GRGCYC	3 cut(s)	241, 1197, 1732
ErhI	CCWWGG	5 cut(s)	388, 734, 1451, 1496, 1556
FaeI	CATG	4 cut(s)	316, 744, 1778, 1853
FalI	AAGNNNNNCTT	2 cut(s)	693, 725
FaqI	GGGAC	1 cut(s)	786
FatI	CATG	4 cut(s)	312, 740, 1774, 1849
FauI	CCCGC	1 cut(s)	613
FauNDI	CATATG	1 cut(s)	1789
FbaI	TGATCA	2 cut(s)	331, 1051
FblI	GTMKAC	1 cut(s)	1065
Fnu4HI	GCNGC	9 cut(s)	128, 283, 338, 618, 686, 1023, 1430, 1469, 1719
FokI	GGATG	5 cut(s)	383, 767, 858, 1343, 1468
FriOI	GRGCYC	3 cut(s)	241, 1197, 1732
Fsp4HI	GCNGC	9 cut(s)	128, 283, 338, 618, 686, 1023, 1430, 1469, 1719
FspBI	CTAG	3 cut(s)	161, 1452, 1557
GlaI	GCGC	3 cut(s)	138, 148, 944
GluI	GCNGC	9 cut(s)	128, 283, 338, 618, 686, 1023, 1430, 1469, 1719
GsuI	CTGGAG	2 cut(s)	1722, 1782
HaeII	RGCGCY	1 cut(s)	140
HaeIII	GGCC	5 cut(s)	5, 53, 65, 1471, 1857
HapII	CCGG	5 cut(s)	75, 121, 234, 1111, 1874
HgaI	GACGC	1 cut(s)	176
HhaI	GCGC	3 cut(s)	139, 149, 945
Hin1II	CATG	4 cut(s)	316, 744, 1778, 1853
Hin6I	GCGC	3 cut(s)	137, 147, 943
HinP1I	GCGC	3 cut(s)	137, 147, 943
HincII	GTYRAC	2 cut(s)	1507, 1837
HindII	GTYRAC	2 cut(s)	1507, 1837
HindIII	AAGCTT	1 cut(s)	326
HinfI	GANTC	5 cut(s)	351, 638, 982, 1313, 1361
HpaI	GTTAAC	2 cut(s)	1507, 1837
HpaII	CCGG	5 cut(s)	75, 121, 234, 1111, 1874
HphI	GGTGA	3 cut(s)	476, 971, 1395
Hpy166II	GTNNAC	8 cut(s)	310, 746, 962, 1066, 1082, 1088, 1507, 1837
Hpy188I	TCNGA	7 cut(s)	109, 356, 457, 787, 1056, 1165, 1267
Hpy8I	GTNNAC	8 cut(s)	310, 746, 962, 1066, 1082, 1088, 1507, 1837
Hpy99I	CGWCG	2 cut(s)	192, 1283
HpyAV	CCTTC	5 cut(s)	55, 919, 1500, 1521, 1770
HpyCH4III	ACNGT	5 cut(s)	155, 275, 1462, 1547, 1619
HpyCH4IV	ACGT	7 cut(s)	84, 572, 657, 1005, 1084, 1370, 1397
HpyF10VI	GCNNNNNNNGC	8 cut(s)	71, 136, 155, 213, 334, 1536, 1727, 1736
HpyF3I	CTNAG	4 cut(s)	7, 324, 1151, 1812
HpySE526I	ACGT	7 cut(s)	84, 572, 657, 1005, 1084, 1370, 1397
Hsp92II	CATG	4 cut(s)	316, 744, 1778, 1853
HspAI	GCGC	3 cut(s)	137, 147, 943
Kpn2I	TCCGGA	2 cut(s)	233, 1110
KroI	GCCGGC	1 cut(s)	74
KroNI	GCCGGC	1 cut(s)	76
Ksp22I	TGATCA	2 cut(s)	331, 1051
KspAI	GTTAAC	2 cut(s)	1507, 1837
Kzo9I	GATC	8 cut(s)	208, 288, 331, 505, 916, 1051, 1570, 1705
Lsp1109I	GCAGC	8 cut(s)	139, 269, 349, 604, 672, 1034, 1416, 1705
LweI	GCATC	3 cut(s)	349, 364, 839
MabI	ACCWGGT	1 cut(s)	1594
MaeI	CTAG	3 cut(s)	161, 1452, 1557
MaeII	ACGT	7 cut(s)	84, 572, 657, 1005, 1084, 1370, 1397
MaeIII	GTNAC	3 cut(s)	1314, 1344, 1541
MalI	GATC	8 cut(s)	210, 290, 333, 507, 918, 1053, 1572, 1707
MboI	GATC	8 cut(s)	208, 288, 331, 505, 916, 1051, 1570, 1705
MflI	RGATCY	1 cut(s)	1705
MhlI	GDGCHC	8 cut(s)	160, 241, 425, 748, 1078, 1197, 1603, 1732
MlyI	GAGTC	4 cut(s)	345, 647, 1322, 1355
MmeI	TCCRAC	1 cut(s)	1360
MreI	CGCCGGCG	1 cut(s)	74
MroI	TCCGGA	2 cut(s)	233, 1110
MroNI	GCCGGC	1 cut(s)	74
MroXI	GAANNNNTTC	2 cut(s)	966, 1522
MseI	TTAA	8 cut(s)	258, 585, 729, 908, 1002, 1092, 1506, 1836
MslI	CAYNNNNRTG	1 cut(s)	1692
MspCI	CTTAAG	1 cut(s)	1091
MspI	CCGG	5 cut(s)	75, 121, 234, 1111, 1874
MspR9I	CCNGG	7 cut(s)	47, 67, 933, 1596, 1726, 1806, 1875
MvaI	CCWGG	6 cut(s)	47, 67, 933, 1596, 1726, 1806
MvnI	CGCG	3 cut(s)	18, 147, 945
MwoI	GCNNNNNNNGC	8 cut(s)	71, 136, 155, 213, 334, 1536, 1727, 1736
NaeI	GCCGGC	1 cut(s)	76
NciI	CCSGG	1 cut(s)	1875
NdeI	CATATG	1 cut(s)	1789
NdeII	GATC	8 cut(s)	208, 288, 331, 505, 916, 1051, 1570, 1705
NgoMIV	GCCGGC	1 cut(s)	74
NlaIII	CATG	4 cut(s)	316, 744, 1778, 1853
NlaIV	GGNNCC	4 cut(s)	386, 774, 930, 1731
NmuCI	GTSAC	2 cut(s)	1314, 1344
NspI	RCATGY	1 cut(s)	744
PaeR7I	CTCGAG	2 cut(s)	240, 509
PaqCI	CACCTGC	1 cut(s)	1777
PasI	CCCWGGG	1 cut(s)	1725
PdiI	GCCGGC	1 cut(s)	76
PdmI	GAANNNNTTC	2 cut(s)	966, 1522
PfeI	GAWTC	1 cut(s)	982
PflMI	CCANNNNNTGG	3 cut(s)	1412, 1805, 1850
PkrI	GCNGC	9 cut(s)	129, 284, 339, 619, 687, 1024, 1431, 1470, 1720
PleI	GAGTC	4 cut(s)	345, 646, 1321, 1355
PpsI	GAGTC	4 cut(s)	345, 646, 1321, 1355
Ppu21I	YACGTR	1 cut(s)	1371
PpuMI	RGGWCCY	1 cut(s)	773
PshBI	ATTAAT	1 cut(s)	258
Psp124BI	GAGCTC	2 cut(s)	241, 1197
Psp1406I	AACGTT	2 cut(s)	84, 572
Psp5II	RGGWCCY	1 cut(s)	773
Psp6I	CCWGG	6 cut(s)	45, 65, 931, 1594, 1724, 1804
PspGI	CCWGG	6 cut(s)	45, 65, 931, 1594, 1724, 1804
PspN4I	GGNNCC	4 cut(s)	386, 774, 930, 1731
PspPI	GGNCC	4 cut(s)	52, 231, 773, 1808
PspPPI	RGGWCCY	1 cut(s)	773
PspXI	VCTCGAGB	1 cut(s)	240
PstI	CTGCAG	1 cut(s)	690
PstNI	CAGNNNCTG	1 cut(s)	1805
PsuI	RGATCY	1 cut(s)	1705
RsaI	GTAC	6 cut(s)	272, 1274, 1567, 1627, 1817, 1880
RsaNI	GTAC	6 cut(s)	271, 1273, 1566, 1626, 1816, 1879
RseI	CAYNNNNRTG	1 cut(s)	1692
SacI	GAGCTC	2 cut(s)	241, 1197
SaqAI	TTAA	8 cut(s)	258, 585, 729, 908, 1002, 1092, 1506, 1836
SatI	GCNGC	9 cut(s)	128, 283, 338, 618, 686, 1023, 1430, 1469, 1719
Sau3AI	GATC	8 cut(s)	208, 288, 331, 505, 916, 1051, 1570, 1705
Sau96I	GGNCC	4 cut(s)	52, 231, 773, 1808
ScaI	AGTACT	2 cut(s)	272, 1274
SchI	GAGTC	4 cut(s)	345, 647, 1322, 1355
ScrFI	CCNGG	7 cut(s)	47, 67, 933, 1596, 1726, 1806, 1875
SduI	GDGCHC	8 cut(s)	160, 241, 425, 748, 1078, 1197, 1603, 1732
SexAI	ACCWGGT	1 cut(s)	1594
SfaNI	GCATC	3 cut(s)	349, 364, 839
SfcI	CTRYAG	1 cut(s)	686
Sfr274I	CTCGAG	2 cut(s)	240, 509
SgrAI	CRCCGGYG	1 cut(s)	74
SinI	GGWCC	3 cut(s)	231, 773, 1808
SlaI	CTCGAG	2 cut(s)	240, 509
SmiMI	CAYNNNNRTG	1 cut(s)	1692
SmlI	CTYRAG	4 cut(s)	240, 509, 749, 1091
SmoI	CTYRAG	4 cut(s)	240, 509, 749, 1091
SnaBI	TACGTA	1 cut(s)	1371
SsiI	CCGC	5 cut(s)	377, 620, 1342, 1442, 1468
SspMI	CTAG	3 cut(s)	161, 1452, 1557
SstI	GAGCTC	2 cut(s)	241, 1197
StyD4I	CCNGG	7 cut(s)	45, 65, 931, 1594, 1724, 1804, 1873
StyI	CCWWGG	5 cut(s)	388, 734, 1451, 1496, 1556
TaaI	ACNGT	5 cut(s)	155, 275, 1462, 1547, 1619
TaiI	ACGT	7 cut(s)	87, 575, 660, 1008, 1087, 1373, 1400
TatI	WGTACW	2 cut(s)	270, 1272
TauI	GCSGC	1 cut(s)	1471
TfiI	GAWTC	1 cut(s)	982
Tru1I	TTAA	8 cut(s)	258, 585, 729, 908, 1002, 1092, 1506, 1836
Tru9I	TTAA	8 cut(s)	258, 585, 729, 908, 1002, 1092, 1506, 1836
TscAI	CASTG	6 cut(s)	37, 430, 771, 1552, 1636, 1657
TseFI	GTSAC	2 cut(s)	1314, 1344
TseI	GCWGC	8 cut(s)	127, 282, 337, 617, 685, 1022, 1429, 1718
Tsp45I	GTSAC	2 cut(s)	1314, 1344
TspDTI	ATGAA	4 cut(s)	938, 959, 995, 1352
TspGWI	ACGGA	3 cut(s)	1007, 1698, 1833
TspRI	CASTG	6 cut(s)	37, 430, 771, 1552, 1636, 1657
Van91I	CCANNNNNTGG	3 cut(s)	1412, 1805, 1850
Vha464I	CTTAAG	1 cut(s)	1091
VneI	GTGCAC	1 cut(s)	744
VpaK11BI	GGWCC	3 cut(s)	231, 773, 1808
VspI	ATTAAT	1 cut(s)	258
XagI	CCTNNNNNAGG	2 cut(s)	59, 1560
XapI	RAATTY	3 cut(s)	357, 431, 904
XceI	RCATGY	1 cut(s)	744
XhoI	CTCGAG	2 cut(s)	240, 509
XmaJI	CCTAGG	2 cut(s)	1451, 1556
XmiI	GTMKAC	1 cut(s)	1065
XmnI	GAANNNNTTC	2 cut(s)	966, 1522
XspI	CTAG	3 cut(s)	161, 1452, 1557
ZrmI	AGTACT	2 cut(s)	272, 1274

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.