Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0003265

Physical Location & Seq

Genomic Coordinates Forward (+)

64041483 .. 64045433

Gene Structure 3951 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G041090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1437 bp

ATGAGCTGCCTTTGTGCTACCTTGCTGAGATTCTTCCTTGCGGATGTAGGTAAAAGCCTCAGTTTCAACATTGAAGGCAATGGGCTTTTGGGCCCTTCCCTATACGAGAAGGGATTAGCTTATTGCTGGTTCGGTGCTCGAGCCAATGTGGGTATATCCAGTGGGAAGTACTGTTTCGGTTGCAAAGTGGTGTCGTTCCAATCTGTTCAAATGGAGGACACTGACCCTGAAGACCGGAATCTTTGTCGGGTTAGTATTTCTAGAGGGGATGATGTGGTGGGAAGCCTTAGGGAAACAAGCCACAGCTTTGCATATGAGGGCACTGGAAAATTCTGGAATGTTATGAGGTCTCCGTGCTATGGTGAGAAGTTTGGGCTTGGAGATACTATTGTTTGTGCAGTCAGTCTTGAAGAAAAGCCCTTGGCTTCCATTTCTTTTTCCAAGAATGGAAAGTGTTTGGGTACGGCGATGAAGTTCAATGCAGCCATGGATCCTGAAGTGAAGAATTTTCAATGGGAATTTGCCTTTTTCCCTCACCTTCTGTTGAAAAATGTGGAAGTGGAGTTGCAGTTCAGTGTTGAAGATGAACTTGTTCTTGAAGATGGATTTAAGCCTTGGGCGGCTGCTCTTGGGGATGGAAATGCGATTGGGGGACCGGTTTTTGATGATCCAAAGGATTGTGAAGTGATGATGATGGTGGGTATGCCTGCCTCAGGCAAAACAACTGGGGCAAAGAAATGGGTGAAAGACCACCCAGAGAAACGATATGTTTTCGTTGGTATAAACATGGTTTTTGACCAAATGAAGGTTAAGGAGAATGATGATGACCTATATCATCTCAAACTTCTGCTAATTAAAGCATGTTATATCTTAGATAGTCTTTCATCCAGAGCAACCGTCAGAGCAGCCACCACTCCTCGCAATTACATTCTTGATAGCACATATCTAAAGAAGGGTGAACGTCAGTATGAACTTAGATTCAATCTCTATGGTTATATTTTATTATTTTCTATTTTTCAGATTGCTGTTGTGGTGTTTCCAAGCCCCGAAGAGGTGAAGGCTCGTTTGCTGCAAAGATTTGATGATTTGGGATTTTTGGTACACGCTGAAATAATGAATGAATTGTTGGCCACTTTTGTTTTACTTGTCAGCAAGGATGTGACTTGTTCAGATGAGCTTTTTGATGAGGTTATGTTCGTGGAACTCAACAGAGACGAACCACATAATGTTTTGGAGGAGATGAAGCAGGAAGAATCTGATTTGAACTCAAATGACTCTTCGCCATATTCCTGCAAAAACTTTGACGAGTCTTATCCTTTTTCACCAAATTATGGGGGGAGTCATGTCTTTCCATTTGCCCATTTATCTGTAAGGACCCATCATAGAGCGCTGTGGCAGCGCAATTGGCACATAAATGACTCACTCAAATGTCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0019899 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0070013

KEGG Pathways

Metabolic & Signaling

ko05164 map05164

Pfam Domains

Protein Families

AAA_33 SPRY

Protein Analysis

478

Amino Acids

53.98

Weight (kDa)

5.24

Isoelectric Point (pI)

37.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPRY	PF00622	81 - 160	1.2e-08	SPRY domain
AAA_33	PF13671	229 - 379	1.4e-12	AAA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003265)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g03980 FvH4_3g03980 FvH4_3g03980 FvH4_3g03980 FvH4_6g02023
malus_domestica	MD05G1325500.v1.1
prunus_persica	Prupe.4G036900_v2.0.a1
pyrus_communis	pycom05g29950 pycom10g25600
rosa_chinensis	RchiOBHm_Chr5g0006311 RchiOBHm_Chr7g0240451
rosa_laevigata	RLG00000031365
rosa_multiflora	Rmu_co8212078.1_g000001 Rmu_co8265987.1_g000001 Rmu_sc0003884.1_g000002 Rmu_sc0005327.1_g000018
rosa_roxburghii	Rroxscaffold_1G00069680 Rroxscaffold_3G00220000 Rroxscaffold_3G00220020 Rroxscaffold_3G00220060
rosa_rugosa	Rorug04G0421000 Rorug07G0335900 Rorug07G0336000 Rorug07G0336100 Rorug07G0336200
rosa_samantha	Rh5AG048900 Rh5BG048000 Rh5BG048100 Rh5CG056300 Rh5DG047200 Rh7AG490300 Rh7AG490400 Rh7BG463200 Rh7CG506700 Rh7DG474300
rosa_wichuraiana	Rw5G004870 Rw7G041090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1331
AciI	CCGC	2 cut(s)	41, 620
AclWI	GGATC	3 cut(s)	485, 498, 662
AcoI	YGGCCR	1 cut(s)	1128
AcsI	RAATTY	3 cut(s)	329, 505, 518
AcuI	CTGAAG	2 cut(s)	249, 516
AfaI	GTAC	3 cut(s)	170, 463, 1101
AfeI	AGCGCT	1 cut(s)	1389
AfiI	CCNNNNNNNGG	5 cut(s)	359, 713, 805, 1051, 1331
AgeI	ACCGGT	1 cut(s)	655
AjuI	GAANNNNNNNTTGG	2 cut(s)	1109, 1141
AloI	GAACNNNNNNTCC	2 cut(s)	554, 586
AluBI	AGCT	4 cut(s)	6, 119, 306, 1177
AluI	AGCT	4 cut(s)	6, 119, 306, 1177
Alw21I	GWGCWC	1 cut(s)	139
Alw26I	GTCTC	2 cut(s)	354, 1206
AlwI	GGATC	3 cut(s)	485, 498, 662
Ama87I	CYCGRG	1 cut(s)	138
Aor51HI	AGCGCT	1 cut(s)	1389
AoxI	GGCC	2 cut(s)	91, 1128
ApaI	GGGCCC	1 cut(s)	95
ApeKI	GCWGC	6 cut(s)	6, 482, 623, 905, 1069, 1396
ApoI	RAATTY	3 cut(s)	329, 505, 518
AsiGI	ACCGGT	1 cut(s)	655
Asp700I	GAANNNNTTC	1 cut(s)	591
AspLEI	GCGC	2 cut(s)	1390, 1401
AspS9I	GGNCC	4 cut(s)	91, 92, 653, 1374
AsuHPI	GGTGA	6 cut(s)	374, 527, 754, 968, 1066, 1314
AvaI	CYCGRG	1 cut(s)	138
AvaII	GGWCC	2 cut(s)	653, 1374
AxyI	CCTNAGG	2 cut(s)	287, 712
BaeGI	GKGCMC	2 cut(s)	95, 323
BaeI	ACNNNNGTAYC	2 cut(s)	375, 408
BalI	TGGCCA	1 cut(s)	1130
BamHI	GGATCC	1 cut(s)	490
BanII	GRGCYC	1 cut(s)	95
BbsI	GAAGAC	1 cut(s)	237
Bbv12I	GWGCWC	1 cut(s)	139
BbvI	GCAGC	5 cut(s)	494, 610, 917, 1056, 1408
BccI	CCATC	4 cut(s)	596, 629, 688, 1386
BceAI	ACGGC	1 cut(s)	480
BcoDI	GTCTC	2 cut(s)	354, 1206
BfaI	CTAG	1 cut(s)	261
BfoI	RGCGCY	1 cut(s)	1391
BisI	GCNGC	7 cut(s)	7, 483, 621, 624, 906, 1070, 1397
BlsI	GCNGC	7 cut(s)	8, 484, 622, 625, 907, 1071, 1398
BmcAI	AGTACT	1 cut(s)	170
Bme18I	GGWCC	2 cut(s)	653, 1374
BmeT110I	CYCGRG	1 cut(s)	138
BmgT120I	GGNCC	4 cut(s)	91, 92, 653, 1374
BmiI	GGNNCC	4 cut(s)	93, 492, 654, 1376
BmrI	ACTGGG	1 cut(s)	735
BmuI	ACTGGG	1 cut(s)	735
BpiI	GAAGAC	1 cut(s)	237
BsaI	GGTCTC	1 cut(s)	354
BsaJI	CCNNGG	3 cut(s)	420, 486, 614
BsaWI	WCCGGW	2 cut(s)	234, 655
BsaXI	ACNNNNNCTCC	2 cut(s)	554, 584
Bsc4I	CCNNNNNNNGG	5 cut(s)	359, 713, 805, 1051, 1331
Bse118I	RCCGGY	1 cut(s)	655
Bse1I	ACTGG	3 cut(s)	159, 328, 730
Bse21I	CCTNAGG	2 cut(s)	287, 712
Bse3DI	GCAATG	1 cut(s)	85
BseDI	CCNNGG	3 cut(s)	420, 486, 614
BseGI	GGATG	5 cut(s)	49, 274, 640, 884, 1162
BseLI	CCNNNNNNNGG	5 cut(s)	359, 713, 805, 1051, 1331
BseMI	GCAATG	1 cut(s)	85
BseMII	CTCAG	3 cut(s)	17, 73, 726
BseNI	ACTGG	3 cut(s)	159, 328, 730
BseRI	GAGGAG	2 cut(s)	906, 1250
BseSI	GKGCMC	2 cut(s)	95, 323
BseXI	GCAGC	5 cut(s)	494, 610, 917, 1056, 1408
BsgI	GTGCAG	1 cut(s)	417
BshFI	GGCC	2 cut(s)	93, 1130
BshTI	ACCGGT	1 cut(s)	655
BsiHKAI	GWGCWC	1 cut(s)	139
BsiHKCI	CYCGRG	1 cut(s)	138
BsiSI	CCGG	2 cut(s)	235, 656
BslFI	GGGAC	1 cut(s)	666
BslI	CCNNNNNNNGG	5 cut(s)	359, 713, 805, 1051, 1331
BsmAI	GTCTC	2 cut(s)	354, 1206
BsmBI	CGTCTC	1 cut(s)	1206
BsmFI	GGGAC	1 cut(s)	666
BsnI	GGCC	2 cut(s)	93, 1130
Bso31I	GGTCTC	1 cut(s)	354
BsoBI	CYCGRG	1 cut(s)	138
Bsp120I	GGGCCC	1 cut(s)	91
Bsp1286I	GDGCHC	3 cut(s)	95, 139, 323
Bsp143I	GATC	2 cut(s)	490, 667
Bsp19I	CCATGG	1 cut(s)	486
BspACI	CCGC	2 cut(s)	41, 620
BspANI	GGCC	2 cut(s)	93, 1130
BspCNI	CTCAG	3 cut(s)	18, 72, 725
BspLI	GGNNCC	4 cut(s)	93, 492, 654, 1376
BspPI	GGATC	3 cut(s)	485, 498, 662
BspTNI	GGTCTC	1 cut(s)	354
BsrDI	GCAATG	1 cut(s)	85
BsrFI	RCCGGY	1 cut(s)	655
BsrI	ACTGG	3 cut(s)	159, 328, 730
BssAI	RCCGGY	1 cut(s)	655
BssECI	CCNNGG	3 cut(s)	420, 486, 614
BssMI	GATC	2 cut(s)	490, 667
BssT1I	CCWWGG	3 cut(s)	420, 486, 614
Bst4CI	ACNGT	2 cut(s)	173, 898
Bst6I	CTCTTC	2 cut(s)	1044, 1282
BstC8I	GCNNGC	1 cut(s)	708
BstDEI	CTNAG	6 cut(s)	26, 59, 287, 712, 871, 974
BstDSI	CCRYGG	1 cut(s)	486
BstENI	CCTNNNNNAGG	1 cut(s)	711
BstF5I	GGATG	5 cut(s)	49, 274, 640, 884, 1162
BstH2I	RGCGCY	1 cut(s)	1391
BstHHI	GCGC	2 cut(s)	1390, 1401
BstKTI	GATC	2 cut(s)	493, 670
BstMAI	GTCTC	2 cut(s)	354, 1206
BstMBI	GATC	2 cut(s)	490, 667
BstMWI	GCNNNNNNNGC	2 cut(s)	1396, 1405
BstNSI	RCATGY	1 cut(s)	864
BstSLI	GKGCMC	2 cut(s)	95, 323
BstV1I	GCAGC	5 cut(s)	494, 610, 917, 1056, 1408
BstV2I	GAAGAC	1 cut(s)	237
BstX2I	RGATCY	1 cut(s)	490
BstYI	RGATCY	1 cut(s)	490
Bsu36I	CCTNAGG	2 cut(s)	287, 712
BsuRI	GGCC	2 cut(s)	93, 1130
BtgI	CCRYGG	1 cut(s)	486
BtgZI	GCGATG	1 cut(s)	482
BtsCI	GGATG	5 cut(s)	49, 274, 640, 884, 1162
BtsIMutI	CAGTG	4 cut(s)	166, 219, 321, 580
Cac8I	GCNNGC	1 cut(s)	708
CfoI	GCGC	2 cut(s)	1390, 1401
Cfr10I	RCCGGY	1 cut(s)	655
Cfr13I	GGNCC	4 cut(s)	91, 92, 653, 1374
Csp6I	GTAC	3 cut(s)	169, 462, 1100
CspAI	ACCGGT	1 cut(s)	655
CviAII	CATG	4 cut(s)	487, 787, 861, 1343
CviQI	GTAC	3 cut(s)	169, 462, 1100
DdeI	CTNAG	6 cut(s)	26, 59, 287, 712, 871, 974
DpnI	GATC	2 cut(s)	492, 669
DpnII	GATC	2 cut(s)	490, 667
EaeI	YGGCCR	1 cut(s)	1128
Eam1104I	CTCTTC	2 cut(s)	1044, 1282
EarI	CTCTTC	2 cut(s)	1044, 1282
Eco130I	CCWWGG	3 cut(s)	420, 486, 614
Eco24I	GRGCYC	1 cut(s)	95
Eco31I	GGTCTC	1 cut(s)	354
Eco47I	GGWCC	2 cut(s)	653, 1374
Eco47III	AGCGCT	1 cut(s)	1389
Eco57I	CTGAAG	2 cut(s)	249, 516
Eco81I	CCTNAGG	2 cut(s)	287, 712
Eco88I	CYCGRG	1 cut(s)	138
EcoNI	CCTNNNNNAGG	1 cut(s)	711
EcoO109I	RGGNCCY	2 cut(s)	92, 1374
EcoT14I	CCWWGG	3 cut(s)	420, 486, 614
EcoT38I	GRGCYC	1 cut(s)	95
ErhI	CCWWGG	3 cut(s)	420, 486, 614
Esp3I	CGTCTC	1 cut(s)	1206
FaeI	CATG	4 cut(s)	490, 790, 864, 1346
FalI	AAGNNNNNCTT	2 cut(s)	573, 605
FaqI	GGGAC	1 cut(s)	666
FatI	CATG	4 cut(s)	486, 786, 860, 1342
FauNDI	CATATG	1 cut(s)	313
Fnu4HI	GCNGC	7 cut(s)	7, 483, 621, 624, 906, 1070, 1397
FokI	GGATG	5 cut(s)	56, 281, 647, 871, 1169
FriOI	GRGCYC	1 cut(s)	95
Fsp4HI	GCNGC	7 cut(s)	7, 483, 621, 624, 906, 1070, 1397
FspBI	CTAG	1 cut(s)	261
GlaI	GCGC	2 cut(s)	1389, 1400
GluI	GCNGC	7 cut(s)	7, 483, 621, 624, 906, 1070, 1397
HaeII	RGCGCY	1 cut(s)	1391
HaeIII	GGCC	2 cut(s)	93, 1130
HapII	CCGG	2 cut(s)	235, 656
HhaI	GCGC	2 cut(s)	1390, 1401
Hin1II	CATG	4 cut(s)	490, 790, 864, 1346
Hin6I	GCGC	2 cut(s)	1388, 1399
HinP1I	GCGC	2 cut(s)	1388, 1399
HinfI	GANTC	8 cut(s)	30, 238, 978, 1253, 1274, 1307, 1339, 1418
HpaII	CCGG	2 cut(s)	235, 656
HphI	GGTGA	6 cut(s)	374, 527, 754, 968, 1066, 1314
Hpy166II	GTNNAC	2 cut(s)	959, 1102
Hpy188I	TCNGA	4 cut(s)	902, 1020, 1171, 1258
Hpy188III	TCNNGA	7 cut(s)	261, 334, 407, 494, 596, 888, 932
Hpy8I	GTNNAC	2 cut(s)	959, 1102
HpyAV	CCTTC	7 cut(s)	68, 103, 105, 548, 799, 946, 1051
HpyCH4III	ACNGT	2 cut(s)	173, 898
HpyCH4IV	ACGT	1 cut(s)	961
HpyCH4V	TGCA	7 cut(s)	183, 311, 398, 482, 568, 1072, 1293
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1396, 1405
HpyF3I	CTNAG	6 cut(s)	26, 59, 287, 712, 871, 974
HpySE526I	ACGT	1 cut(s)	961
Hsp92II	CATG	4 cut(s)	490, 790, 864, 1346
HspAI	GCGC	2 cut(s)	1388, 1399
Kzo9I	GATC	2 cut(s)	490, 667
Lsp1109I	GCAGC	5 cut(s)	494, 610, 917, 1056, 1408
MaeI	CTAG	1 cut(s)	261
MaeII	ACGT	1 cut(s)	961
MaeIII	GTNAC	1 cut(s)	1159
MalI	GATC	2 cut(s)	492, 669
MboI	GATC	2 cut(s)	490, 667
MboII	GAAGA	9 cut(s)	25, 242, 422, 514, 593, 611, 1061, 1262, 1269
MfeI	CAATTG	1 cut(s)	1402
MflI	RGATCY	1 cut(s)	490
MhlI	GDGCHC	3 cut(s)	95, 139, 323
MlsI	TGGCCA	1 cut(s)	1130
MluCI	AATT	8 cut(s)	329, 505, 518, 852, 922, 1121, 1327, 1402
MluNI	TGGCCA	1 cut(s)	1130
MlyI	GAGTC	4 cut(s)	1268, 1316, 1348, 1412
Mox20I	TGGCCA	1 cut(s)	1130
MroXI	GAANNNNTTC	1 cut(s)	591
MscI	TGGCCA	1 cut(s)	1130
MseI	TTAA	3 cut(s)	609, 810, 855
MslI	CAYNNNNRTG	2 cut(s)	1413, 1426
Msp20I	TGGCCA	1 cut(s)	1130
MspI	CCGG	2 cut(s)	235, 656
MunI	CAATTG	1 cut(s)	1402
MwoI	GCNNNNNNNGC	2 cut(s)	1396, 1405
NcoI	CCATGG	1 cut(s)	486
NdeI	CATATG	1 cut(s)	313
NdeII	GATC	2 cut(s)	490, 667
NlaIII	CATG	4 cut(s)	490, 790, 864, 1346
NlaIV	GGNNCC	4 cut(s)	93, 492, 654, 1376
NmuCI	GTSAC	1 cut(s)	1159
NspI	RCATGY	1 cut(s)	864
PaeR7I	CTCGAG	1 cut(s)	138
PdmI	GAANNNNTTC	1 cut(s)	591
PfeI	GAWTC	4 cut(s)	30, 238, 978, 1253
PflMI	CCANNNNNTGG	1 cut(s)	1331
PinAI	ACCGGT	1 cut(s)	655
PkrI	GCNGC	7 cut(s)	8, 484, 622, 625, 907, 1071, 1398
PleI	GAGTC	4 cut(s)	1268, 1315, 1347, 1412
PpsI	GAGTC	4 cut(s)	1268, 1315, 1347, 1412
PpuMI	RGGWCCY	1 cut(s)	1374
Psp5II	RGGWCCY	1 cut(s)	1374
PspN4I	GGNNCC	4 cut(s)	93, 492, 654, 1376
PspOMI	GGGCCC	1 cut(s)	91
PspPI	GGNCC	4 cut(s)	91, 92, 653, 1374
PspPPI	RGGWCCY	1 cut(s)	1374
PspXI	VCTCGAGB	1 cut(s)	138
PsuI	RGATCY	1 cut(s)	490
RsaI	GTAC	3 cut(s)	170, 463, 1101
RsaNI	GTAC	3 cut(s)	169, 462, 1100
RseI	CAYNNNNRTG	2 cut(s)	1413, 1426
SaqAI	TTAA	3 cut(s)	609, 810, 855
SatI	GCNGC	7 cut(s)	7, 483, 621, 624, 906, 1070, 1397
Sau3AI	GATC	2 cut(s)	490, 667
Sau96I	GGNCC	4 cut(s)	91, 92, 653, 1374
ScaI	AGTACT	1 cut(s)	170
SchI	GAGTC	4 cut(s)	1268, 1316, 1348, 1412
SduI	GDGCHC	3 cut(s)	95, 139, 323
Sfr274I	CTCGAG	1 cut(s)	138
SinI	GGWCC	2 cut(s)	653, 1374
SlaI	CTCGAG	1 cut(s)	138
SmiMI	CAYNNNNRTG	2 cut(s)	1413, 1426
SmlI	CTYRAG	1 cut(s)	138
SmoI	CTYRAG	1 cut(s)	138
Sse9I	AATT	8 cut(s)	329, 505, 518, 852, 922, 1121, 1327, 1402
SsiI	CCGC	2 cut(s)	41, 620
SspMI	CTAG	1 cut(s)	261
StyI	CCWWGG	3 cut(s)	420, 486, 614
TaaI	ACNGT	2 cut(s)	173, 898
TaiI	ACGT	1 cut(s)	964
TaqI	TCGA	1 cut(s)	139
TasI	AATT	8 cut(s)	329, 505, 518, 852, 922, 1121, 1327, 1402
TatI	WGTACW	1 cut(s)	168
TauI	GCSGC	1 cut(s)	623
TfiI	GAWTC	4 cut(s)	30, 238, 978, 1253
Tru1I	TTAA	3 cut(s)	609, 810, 855
Tru9I	TTAA	3 cut(s)	609, 810, 855
TscAI	CASTG	4 cut(s)	166, 226, 328, 580
TseFI	GTSAC	1 cut(s)	1159
TseI	GCWGC	6 cut(s)	6, 482, 623, 905, 1069, 1396
Tsp45I	GTSAC	1 cut(s)	1159
TspDTI	ATGAA	8 cut(s)	485, 600, 818, 873, 984, 1130, 1134, 1256
TspGWI	ACGGA	1 cut(s)	342
TspRI	CASTG	4 cut(s)	166, 226, 328, 580
Van91I	CCANNNNNTGG	1 cut(s)	1331
VpaK11BI	GGWCC	2 cut(s)	653, 1374
XagI	CCTNNNNNAGG	1 cut(s)	711
XapI	RAATTY	3 cut(s)	329, 505, 518
XbaI	TCTAGA	1 cut(s)	260
XceI	RCATGY	1 cut(s)	864
XhoI	CTCGAG	1 cut(s)	138
XmnI	GAANNNNTTC	1 cut(s)	591
XspI	CTAG	1 cut(s)	261
ZrmI	AGTACT	1 cut(s)	170

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.