MD05G1345000.v1.1

GPI-anchored protein At1g61900-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009727
Physical Location & Seq
Reverse (-)
46479600 .. 46481200
1601 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1345000.v1.1.491

Sequence Viewer

Length: 414 bp
ATGGAGAGCTATGTGGCCCACTTGCAAAAGCAGAGTCTCATAACCAACTTGCAAGCTTTGGATTGTGCTGCATCACTGGGAATGAAGTTAAGAAAGTCAAATATTATTGAAGATGTGTATAGCCTATGTCGTATAAGCCTCAAGGATTTCTCTCTTCAAGTGTCTGGATGTCTTTTGCCGAGCCTGCCTTCCGATGCAACATTTGACAGTTCAGGAGTCAGCTTCCTTTGTGATTTGAATGATAACATTCCAGCTCCATGGCCCACCTCTCAAGGGGCAGCGTCCTCGTGCAGTAAAAATGTCAAGATTCCTGCACTTCCTGCAGCAGCATCTGCTGGAAATGGTCTGTACCATGATAATGTGATGCTTTCTCTACTCCTTGCTTCCGGGATGGTCCTTATGATGCTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

14.51

Weight (kDa)

5.46

Isoelectric Point (pI)

53.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At1g61900 PF26584 1 - 44 1.5e-20 At1g61900-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 273
AgsI TTSAA 3 cut(s) 110, 158, 238
AluBI AGCT 4 cut(s) 9, 56, 222, 254
AluI AGCT 4 cut(s) 9, 56, 222, 254
Alw26I GTCTC 1 cut(s) 41
AlwNI CAGNNNCTG 1 cut(s) 332
AoxI GGCC 2 cut(s) 15, 260
ApeKI GCWGC 4 cut(s) 68, 278, 323, 326
ArsI GACNNNNNNTTYG 2 cut(s) 19, 51
AspS9I GGNCC 3 cut(s) 16, 261, 394
AsuC2I CCSGG 1 cut(s) 388
AvaII GGWCC 1 cut(s) 394
BauI CACGAG 1 cut(s) 286
BbvI GCAGC 4 cut(s) 55, 290, 335, 338
BccI CCATC 1 cut(s) 385
BcgI CGANNNNNNTGC 2 cut(s) 267, 301
BcnI CCSGG 1 cut(s) 388
BcoDI GTCTC 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 321
BisI GCNGC 4 cut(s) 69, 279, 324, 327
BlsI GCNGC 4 cut(s) 70, 280, 325, 328
Bme1390I CCNGG 1 cut(s) 388
Bme18I GGWCC 1 cut(s) 394
BmgT120I GGNCC 3 cut(s) 16, 261, 394
BmrFI CCNGG 1 cut(s) 388
BmrI ACTGGG 1 cut(s) 86
BmsI GCATC 5 cut(s) 80, 184, 338, 354, 393
BmuI ACTGGG 1 cut(s) 86
BpuEI CTTGAG 2 cut(s) 125, 255
BpuMI CCSGG 1 cut(s) 388
BsaJI CCNNGG 1 cut(s) 257
Bsc4I CCNNNNNNNGG 1 cut(s) 273
Bse1I ACTGG 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 257
BseGI GGATG 2 cut(s) 173, 396
BseLI CCNNNNNNNGG 1 cut(s) 273
BseNI ACTGG 1 cut(s) 81
BseXI GCAGC 4 cut(s) 55, 290, 335, 338
BsgI GTGCAG 2 cut(s) 297, 310
BshFI GGCC 2 cut(s) 17, 262
BsiSI CCGG 1 cut(s) 387
BslI CCNNNNNNNGG 1 cut(s) 273
BsmAI GTCTC 1 cut(s) 41
BsnI GGCC 2 cut(s) 17, 262
Bsp19I CCATGG 1 cut(s) 257
BspANI GGCC 2 cut(s) 17, 262
BspMAI CTGCAG 1 cut(s) 325
BsrI ACTGG 1 cut(s) 81
BssECI CCNNGG 1 cut(s) 257
BssSI CACGAG 1 cut(s) 286
BssT1I CCWWGG 1 cut(s) 257
Bst2BI CACGAG 1 cut(s) 286
Bst4CI ACNGT 1 cut(s) 209
Bst6I CTCTTC 1 cut(s) 159
BstAPI GCANNNNNTGC 2 cut(s) 320, 332
BstC8I GCNNGC 2 cut(s) 54, 185
BstDSI CCRYGG 1 cut(s) 257
BstF5I GGATG 2 cut(s) 173, 396
BstMAI GTCTC 1 cut(s) 41
BstMWI GCNNNNNNNGC 3 cut(s) 184, 320, 332
BstSCI CCNGG 1 cut(s) 386
BstSFI CTRYAG 1 cut(s) 321
BstV1I GCAGC 4 cut(s) 55, 290, 335, 338
BstXI CCANNNNNNTGG 1 cut(s) 258
BsuRI GGCC 2 cut(s) 17, 262
BtgI CCRYGG 1 cut(s) 257
BtsCI GGATG 2 cut(s) 173, 396
BtsIMutI CAGTG 1 cut(s) 74
Cac8I GCNNGC 2 cut(s) 54, 185
CaiI CAGNNNCTG 1 cut(s) 332
Cfr13I GGNCC 3 cut(s) 16, 261, 394
CseI GACGC 1 cut(s) 270
Csp6I GTAC 1 cut(s) 349
CviAII CATG 2 cut(s) 258, 353
CviJI RGCY 9 cut(s) 9, 17, 56, 123, 138, 183, 222, 254, 262
CviKI_1 RGCY 9 cut(s) 9, 17, 56, 123, 138, 183, 222, 254, 262
CviQI GTAC 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 257
FaeI CATG 2 cut(s) 261, 356
FaiI YATR 8 cut(s) 12, 41, 120, 127, 134, 259, 354, 401
FatI CATG 2 cut(s) 257, 352
Fnu4HI GCNGC 4 cut(s) 69, 279, 324, 327
FokI GGATG 2 cut(s) 180, 403
Fsp4HI GCNGC 4 cut(s) 69, 279, 324, 327
GluI GCNGC 4 cut(s) 69, 279, 324, 327
HaeIII GGCC 2 cut(s) 17, 262
HapII CCGG 1 cut(s) 387
HgaI GACGC 1 cut(s) 270
Hin1II CATG 2 cut(s) 261, 356
HindIII AAGCTT 1 cut(s) 54
HinfI GANTC 3 cut(s) 34, 216, 307
HpaII CCGG 1 cut(s) 387
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 3 cut(s) 165, 213, 304
HpyAV CCTTC 1 cut(s) 198
HpyCH4III ACNGT 1 cut(s) 209
HpyCH4V TGCA 7 cut(s) 25, 52, 71, 197, 291, 314, 323
HpyF10VI GCNNNNNNNGC 3 cut(s) 184, 320, 332
Hsp92II CATG 2 cut(s) 261, 356
LmnI GCTCC 1 cut(s) 259
LpnPI CCDG 9 cut(s) 62, 150, 197, 198, 264, 321, 324, 333, 400
Lsp1109I GCAGC 4 cut(s) 55, 290, 335, 338
LweI GCATC 5 cut(s) 80, 184, 338, 354, 393
MboII GAAGA 2 cut(s) 122, 146
MlyI GAGTC 2 cut(s) 43, 225
MnlI CCTC 3 cut(s) 149, 277, 295
MseI TTAA 1 cut(s) 89
MslI CAYNNNNRTG 1 cut(s) 357
MspI CCGG 1 cut(s) 387
MspR9I CCNGG 1 cut(s) 388
MwoI GCNNNNNNNGC 3 cut(s) 184, 320, 332
NciI CCSGG 1 cut(s) 388
NcoI CCATGG 1 cut(s) 257
NlaIII CATG 2 cut(s) 261, 356
NmeAIII GCCGAG 1 cut(s) 204
PfeI GAWTC 1 cut(s) 307
PfoI TCCNGGA 1 cut(s) 386
PkrI GCNGC 4 cut(s) 70, 280, 325, 328
PleI GAGTC 2 cut(s) 42, 224
PpsI GAGTC 2 cut(s) 42, 224
PspPI GGNCC 3 cut(s) 16, 261, 394
PstI CTGCAG 1 cut(s) 325
PstNI CAGNNNCTG 1 cut(s) 332
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
RseI CAYNNNNRTG 1 cut(s) 357
SaqAI TTAA 1 cut(s) 89
SatI GCNGC 4 cut(s) 69, 279, 324, 327
Sau96I GGNCC 3 cut(s) 16, 261, 394
SchI GAGTC 2 cut(s) 43, 225
ScrFI CCNGG 1 cut(s) 388
SetI ASST 5 cut(s) 11, 58, 224, 256, 269
SfaNI GCATC 5 cut(s) 80, 184, 338, 354, 393
SfcI CTRYAG 1 cut(s) 321
SinI GGWCC 1 cut(s) 394
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 2 cut(s) 140, 270
SmoI CTYRAG 2 cut(s) 140, 270
SspI AATATT 1 cut(s) 103
StyD4I CCNGG 1 cut(s) 386
StyI CCWWGG 1 cut(s) 257
TaaI ACNGT 1 cut(s) 209
TfiI GAWTC 1 cut(s) 307
Tru1I TTAA 1 cut(s) 89
Tru9I TTAA 1 cut(s) 89
TscAI CASTG 1 cut(s) 81
TseI GCWGC 4 cut(s) 68, 278, 323, 326
TspDTI ATGAA 1 cut(s) 98
TspRI CASTG 1 cut(s) 81
VpaK11BI GGWCC 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.