RLG00000028318

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0009727
Physical Location & Seq
Reverse (-)
21371030 .. 21372854
1825 bp
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UTR
Exon/CDS
Intron
RLM00000028318

Sequence Viewer

Length: 561 bp
ATGAGGATCTGCAACGCTAGAGATATTGATGAGGATCTACACAGCCGGTGGCTAGCTGTCTATGGCCGAAAGACGATGAGGAGACTCTTCAATTCGAATGTTCTGATCTCAGGGATCCCGGGGGTTTTCGGAATTCGAGGGAGCTGTGACAAGGCTCTCTCTATTTTCTCTAAGTGCAAATTTGAGGCACCGGTGAAGAGCTCTGATGACTCTTCCGGCGATGAGGTCAAAGAAATCGTCTCTCTCTCCTCCTTGCCTTCATCTGCGACCAGTTCTCCATCGTTGGCCGGGTTCGATGGCCTGAAGATTCAGTGTGCTGATCAAAACCTCCTCTTCGTCGATGACAACGGCTGGACTCACGAAGTTCTGGTCGAATTACTACTAGTCCTCAACCGCAACTGGAAAATGTTTGATGCCTTCGAGAATGCAGGAGCGCCGATGACCGAGTCCGATATTGCCAGATTCTGCTCAAGAATTTCTATTGCAGTCTCGAACGCGAGCTTGGCCCAACAAGATATATCGCTGATTCGAACCTTCTCGGGCCACCAAATCTCAATCTGA

Protein Analysis

187

Amino Acids

20.42

Weight (kDa)

5.6

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANKRD13_C PF11904 94 - 178 4.1e-07 ANKRD13 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 187
AccII CGCG 1 cut(s) 497
AciI CCGC 1 cut(s) 394
AclWI GGATC 4 cut(s) 14, 42, 109, 122
AcoI YGGCCR 2 cut(s) 64, 285
AcsI RAATTY 3 cut(s) 132, 179, 474
AcuI CTGAAG 1 cut(s) 323
AgeI ACCGGT 1 cut(s) 190
AgsI TTSAA 1 cut(s) 91
AhlI ACTAGT 1 cut(s) 382
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
AluBI AGCT 4 cut(s) 56, 144, 201, 501
AluI AGCT 4 cut(s) 56, 144, 201, 501
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 3 cut(s) 76, 244, 493
AlwI GGATC 4 cut(s) 14, 42, 109, 122
AlwNI CAGNNNCTG 1 cut(s) 465
Ama87I CYCGRG 2 cut(s) 118, 538
AoxI GGCC 5 cut(s) 64, 285, 298, 504, 541
ApoI RAATTY 3 cut(s) 132, 179, 474
ArsI GACNNNNNNTTYG 4 cut(s) 222, 254, 354, 386
AsiGI ACCGGT 1 cut(s) 190
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 2 cut(s) 505, 541
AsuC2I CCSGG 3 cut(s) 119, 120, 289
AsuHPI GGTGA 1 cut(s) 205
AsuII TTCGAA 2 cut(s) 95, 529
AsuNHI GCTAGC 1 cut(s) 52
AvaI CYCGRG 2 cut(s) 118, 538
BamHI GGATCC 1 cut(s) 114
BanI GGYRCC 1 cut(s) 187
BanII GRGCYC 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 203
BccI CCATC 2 cut(s) 286, 290
BceAI ACGGC 1 cut(s) 364
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 3 cut(s) 119, 120, 289
BcoDI GTCTC 3 cut(s) 76, 244, 493
BcuI ACTAGT 1 cut(s) 382
BfaI CTAG 3 cut(s) 18, 53, 383
BfoI RGCGCY 1 cut(s) 437
Bme1390I CCNGG 3 cut(s) 119, 120, 289
BmeT110I CYCGRG 2 cut(s) 118, 538
BmgT120I GGNCC 2 cut(s) 505, 541
BmiI GGNNCC 2 cut(s) 116, 189
BmrFI CCNGG 3 cut(s) 119, 120, 289
BmsI GCATC 1 cut(s) 403
BmtI GCTAGC 1 cut(s) 56
Bpu14I TTCGAA 2 cut(s) 95, 529
BpuEI CTTGAG 1 cut(s) 454
BpuMI CCSGG 3 cut(s) 119, 120, 289
BsaBI GATNNNNATC 1 cut(s) 33
BsaJI CCNNGG 2 cut(s) 118, 119
BsaWI WCCGGW 1 cut(s) 190
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bse118I RCCGGY 2 cut(s) 45, 190
Bse1I ACTGG 2 cut(s) 270, 404
Bse8I GATNNNNATC 1 cut(s) 33
BseDI CCNNGG 2 cut(s) 118, 119
BseJI GATNNNNATC 1 cut(s) 33
BseMII CTCAG 1 cut(s) 123
BseNI ACTGG 2 cut(s) 270, 404
BseRI GAGGAG 3 cut(s) 94, 238, 320
Bsh1236I CGCG 1 cut(s) 497
BshFI GGCC 5 cut(s) 66, 287, 300, 506, 543
BshNI GGYRCC 1 cut(s) 187
BshTI ACCGGT 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 203
BsiHKCI CYCGRG 2 cut(s) 118, 538
BsiSI CCGG 5 cut(s) 46, 119, 191, 216, 288
BsmAI GTCTC 3 cut(s) 76, 244, 493
BsmBI CGTCTC 1 cut(s) 244
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 5 cut(s) 66, 287, 300, 506, 543
BsoBI CYCGRG 2 cut(s) 118, 538
Bsp119I TTCGAA 2 cut(s) 95, 529
Bsp1286I GDGCHC 1 cut(s) 203
Bsp143I GATC 5 cut(s) 6, 34, 105, 114, 319
BspACI CCGC 1 cut(s) 394
BspANI GGCC 5 cut(s) 66, 287, 300, 506, 543
BspCNI CTCAG 1 cut(s) 122
BspFNI CGCG 1 cut(s) 497
BspLI GGNNCC 2 cut(s) 116, 189
BspOI GCTAGC 1 cut(s) 56
BspPI GGATC 4 cut(s) 14, 42, 109, 122
BspQI GCTCTTC 1 cut(s) 191
BspT104I TTCGAA 2 cut(s) 95, 529
BspT107I GGYRCC 1 cut(s) 187
BsrFI RCCGGY 2 cut(s) 45, 190
BsrI ACTGG 2 cut(s) 270, 404
BssAI RCCGGY 2 cut(s) 45, 190
BssECI CCNNGG 2 cut(s) 118, 119
BssMI GATC 5 cut(s) 6, 34, 105, 114, 319
Bst6I CTCTTC 4 cut(s) 92, 191, 217, 338
BstBI TTCGAA 2 cut(s) 95, 529
BstC8I GCNNGC 2 cut(s) 54, 499
BstDEI CTNAG 2 cut(s) 109, 171
BstFNI CGCG 1 cut(s) 497
BstH2I RGCGCY 1 cut(s) 437
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 5 cut(s) 9, 37, 108, 117, 322
BstMAI GTCTC 3 cut(s) 76, 244, 493
BstMBI GATC 5 cut(s) 6, 34, 105, 114, 319
BstMWI GCNNNNNNNGC 1 cut(s) 503
BstSCI CCNGG 3 cut(s) 117, 118, 287
BstUI CGCG 1 cut(s) 497
BstX2I RGATCY 3 cut(s) 6, 34, 114
BstYI RGATCY 3 cut(s) 6, 34, 114
BsuRI GGCC 5 cut(s) 66, 287, 300, 506, 543
BtgZI GCGATG 1 cut(s) 234
BtsIMutI CAGTG 1 cut(s) 317
Cac8I GCNNGC 2 cut(s) 54, 499
CaiI CAGNNNCTG 1 cut(s) 465
CfoI GCGC 1 cut(s) 436
Cfr10I RCCGGY 2 cut(s) 45, 190
Cfr13I GGNCC 2 cut(s) 505, 541
Cfr9I CCCGGG 1 cut(s) 118
CspAI ACCGGT 1 cut(s) 190
DdeI CTNAG 2 cut(s) 109, 171
DpnI GATC 5 cut(s) 8, 36, 107, 116, 321
DpnII GATC 5 cut(s) 6, 34, 105, 114, 319
EaeI YGGCCR 2 cut(s) 64, 285
Eam1104I CTCTTC 4 cut(s) 92, 191, 217, 338
EarI CTCTTC 4 cut(s) 92, 191, 217, 338
Ecl136II GAGCTC 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 203
Eco53kI GAGCTC 1 cut(s) 201
Eco57I CTGAAG 1 cut(s) 323
Eco88I CYCGRG 2 cut(s) 118, 538
EcoICRI GAGCTC 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 203
Esp3I CGTCTC 1 cut(s) 244
FaiI YATR 2 cut(s) 63, 518
FbaI TGATCA 1 cut(s) 319
FriOI GRGCYC 1 cut(s) 203
FspBI CTAG 3 cut(s) 18, 53, 383
GlaI GCGC 1 cut(s) 435
HaeII RGCGCY 1 cut(s) 437
HaeIII GGCC 5 cut(s) 66, 287, 300, 506, 543
HapII CCGG 5 cut(s) 46, 119, 191, 216, 288
HhaI GCGC 1 cut(s) 436
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HinfI GANTC 7 cut(s) 84, 209, 307, 355, 446, 462, 526
HpaII CCGG 5 cut(s) 46, 119, 191, 216, 288
HphI GGTGA 1 cut(s) 205
Hpy188I TCNGA 5 cut(s) 105, 131, 205, 451, 560
Hpy188III TCNNGA 4 cut(s) 359, 421, 471, 490
Hpy99I CGWCG 1 cut(s) 341
HpyAV CCTTC 3 cut(s) 267, 427, 544
HpyCH4V TGCA 4 cut(s) 12, 177, 428, 485
HpyF10VI GCNNNNNNNGC 1 cut(s) 503
HpyF3I CTNAG 2 cut(s) 109, 171
HspAI GCGC 1 cut(s) 434
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 5 cut(s) 6, 34, 105, 114, 319
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 2 cut(s) 141, 431
LweI GCATC 1 cut(s) 403
MaeI CTAG 3 cut(s) 18, 53, 383
MaeIII GTNAC 1 cut(s) 146
MalI GATC 5 cut(s) 8, 36, 107, 116, 321
MboI GATC 5 cut(s) 6, 34, 105, 114, 319
MboII GAAGA 5 cut(s) 79, 204, 208, 316, 325
MflI RGATCY 3 cut(s) 6, 34, 114
MhlI GDGCHC 1 cut(s) 203
MluCI AATT 5 cut(s) 91, 132, 179, 374, 474
MlyI GAGTC 4 cut(s) 78, 203, 349, 455
MnlI CCTC 9 cut(s) 25, 72, 131, 178, 217, 259, 338, 341, 398
MspI CCGG 5 cut(s) 46, 119, 191, 216, 288
MspR9I CCNGG 3 cut(s) 119, 120, 289
Mva1269I GAATGC 1 cut(s) 430
MvnI CGCG 1 cut(s) 497
MwoI GCNNNNNNNGC 1 cut(s) 503
NciI CCSGG 3 cut(s) 119, 120, 289
NdeII GATC 5 cut(s) 6, 34, 105, 114, 319
NheI GCTAGC 1 cut(s) 52
NlaIV GGNNCC 2 cut(s) 116, 189
NmuCI GTSAC 1 cut(s) 146
NspV TTCGAA 2 cut(s) 95, 529
PciSI GCTCTTC 1 cut(s) 191
PctI GAATGC 1 cut(s) 430
PfeI GAWTC 3 cut(s) 307, 462, 526
PflFI GACNNNGTC 1 cut(s) 445
PinAI ACCGGT 1 cut(s) 190
PleI GAGTC 4 cut(s) 78, 203, 349, 454
PpsI GAGTC 4 cut(s) 78, 203, 349, 454
Psp124BI GAGCTC 1 cut(s) 203
PspN4I GGNNCC 2 cut(s) 116, 189
PspPI GGNCC 2 cut(s) 505, 541
PstNI CAGNNNCTG 1 cut(s) 465
PsuI RGATCY 3 cut(s) 6, 34, 114
PsyI GACNNNGTC 1 cut(s) 445
SacI GAGCTC 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 191
Sau3AI GATC 5 cut(s) 6, 34, 105, 114, 319
Sau96I GGNCC 2 cut(s) 505, 541
SchI GAGTC 4 cut(s) 78, 203, 349, 455
ScrFI CCNGG 3 cut(s) 119, 120, 289
SduI GDGCHC 1 cut(s) 203
SetI ASST 7 cut(s) 58, 146, 203, 228, 330, 503, 536
SfaNI GCATC 1 cut(s) 403
SfuI TTCGAA 2 cut(s) 95, 529
SgrAI CRCCGGYG 1 cut(s) 190
SmaI CCCGGG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
SpeI ACTAGT 1 cut(s) 382
Sse9I AATT 5 cut(s) 91, 132, 179, 374, 474
SsiI CCGC 1 cut(s) 394
SspMI CTAG 3 cut(s) 18, 53, 383
SstI GAGCTC 1 cut(s) 203
StyD4I CCNGG 3 cut(s) 117, 118, 287
TaqI TCGA 8 cut(s) 95, 136, 294, 339, 372, 420, 491, 529
TaqII GACCGA 1 cut(s) 458
TasI AATT 5 cut(s) 91, 132, 179, 374, 474
TfiI GAWTC 3 cut(s) 307, 462, 526
TscAI CASTG 1 cut(s) 317
TseFI GTSAC 1 cut(s) 146
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 1 cut(s) 249
TspMI CCCGGG 1 cut(s) 118
TspRI CASTG 1 cut(s) 317
Tth111I GACNNNGTC 1 cut(s) 445
XapI RAATTY 3 cut(s) 132, 179, 474
XmaI CCCGGG 1 cut(s) 118
XspI CTAG 3 cut(s) 18, 53, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.