Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0005778

Physical Location & Seq

Genomic Coordinates Forward (+)

33132835 .. 33136528

Gene Structure 3694 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1197300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1695 bp

ATGGAGCCGAAGGAGCTGAATTTGAACAGGGACTATGAAACTGGTAGCAGTGGCGGCGGGGGTGGAGGTTGTGGCGGCGGCGGTGGTGGTGGTGCTGATGGGTTCATCGACAGGAGCAGAGTGAGGATTTTACTGTGCGATAACAACGGGTCCAGTTCGGAAGAGGTTTTCACGCTTCTTGTGAAATGCTCTTACCAGGTTATTTCCGTGAAGTCTCCTCGACAGGTGATTGATGCATTGAATGCAGAGGGACCTGATATCGATCTCATACTTGCCGAAGTTGACCTTCCAATGCGCAAAGGCATGAAATTGTTGAAGTACATCACACGGGACAGAGAGTTAAGGCGCATTCCTGTAATCATGATGTCGGCAGAAGATGAGGTCTCTACTGTTGTGAAGTGCTTGAAGCTTGGAGCAGCAGACTATCTTGTAAAGCCTTTACGAACTAATGAGCTTCTGAACTTGTGGACGCACATGTGGAGAAGAAGGCGCATGCTTGGGCTGGCAGAGAAGAACCTTATAAATTATGTTGATCTGGTGATATCAGACCCTAGCGGATCCAATACGAACAGTACTACTTTATTCTCGGATGACGCAGATGATAAATCTGGCAATCCCGAGACAGGAACCTCAGCTCCTGAGGAAGATAAGTCTGCTGCTGTTGCTGCTGCTATGGGGCTTCCGGTCAAGAGAATATCAGAATTTCAGCCTGATGATCCAGGAATAAGTGACCACCAAACTGGAAAGTTTTTATCTGCCCCAAGAAAGAGTAAACTAAAGATTGGCATTGGCGAGTCATCTGCCTTCTTTACCTATGTCAAATCAAGCAAACTTAAAATCAACTCCCAGGTGGTTGCCCATGTTGAAGACATTGCCACTGAACATTTGAGGATAGAAGAGAAACATCAAGAATTTGTTTGCCACCAAGTGGTTGATGATGATCCCCAAGTACATGGAAATGGAGAGGCGTGGGAAAGCAACTCACAGGGAGATGACTTGCCAAGTAGTAATAGTATCCCAGATTCTCTTTCGTTGGAGAGGTCTAGTACCCCGTCTGGATCAATGGAACTTCAACATCAGAGAAGTTTTGAGGAAGACAGATCCTCTCAAGTGCCTGCACATCCAAGAAATGAACTTCAACATGATTTTTCTGGCTTACCTGCCCAAGCCGCCTATCAATATTATATGCCGGGGGCTGTCAATCAAGTTATGATGTCATCGTCACCCCAAGTTTATCAAAAGAATCTGCAAGACATGCAAAATCATGCTATGATGCCACAATACAATCATCTTCCGCGCTGCCCTCCACACATAAATGGGATGGCTTCGTTCCCCTATTATAGTATATGCATGCATCCTGGTCAACAAATGCCGAATGCTCAGCCATGGCCCTCATTTGGAAGTTCAGCTTCCACTGAAGTGAATCCAAATAAGGTTGACAGAAGGGAGGCAGCATTGATTAAATTTAGGCAAAAAAGAAAGGAGCGGTGTTTTGATAAAAAGATCAGGTATGTAAATCGAAAGAAACTTGCCGAAAGGAGGCCTCGTGTCCGGGGACAGTTTGTGAGGAAGTTAAATGGTGTAAATGTGGATCTTAACGGCGAACCTGCATCTGTTGAAGATGATGAGGAGGATGAAAGGGACGACGAGGAGCTTGCATCAAGGGATTCCTCACCGGAAGATGGTGCTCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006725 GO:0006807 GO:0007623 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009892 GO:0009987 GO:0010031 GO:0010467 GO:0010468 GO:0010556 GO:0010605 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0032501 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0048511 GO:0048519 GO:0050789 GO:0050794 GO:0050879 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04712 map04712

Pfam Domains

Protein Families

CCT Response_reg

Protein Analysis

565

Amino Acids

62.62

Weight (kDa)

5.58

Isoelectric Point (pI)

51.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Response_reg	PF00072	43 - 154	8.8e-18	Response regulator receiver domain
CCT	PF06203	482 - 524	2.4e-15	CCT motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005778)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61380
fragaria_vesca	FvH4_5g14560
malus_domestica	MD06G1197300.v1.1 MD14G1204700.v1.1
prunus_persica	Prupe.5G197900_v2.0.a1 Prupe.5G198000_v2.0.a1
pyrus_communis	pycom06g17420 pycom06g17430 pycom14g16910
rosa_chinensis	RchiOBHm_Chr7g0182001
rosa_laevigata	RLG00000005156 RLG00000005161
rosa_multiflora	Rmu_co8226389.1_g000001 Rmu_sc0003511.1_g000044 Rmu_sc0017796.1_g000001 Rmu_ssc0000297.1_g000003
rosa_roxburghii	Rroxscaffold_3G00271630
rosa_rugosa	Rorug06G0445000 Rorug06G0445100 Rorug06G0445300
rosa_samantha	Rh7AG047400 Rh7BG047400 Rh7BG047500 Rh7CG047700 Rh7DG046700
rosa_wichuraiana	Rw0G000460 Rw0G019690 Rw7G003930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	521
Acc16I	TGCGCA	1 cut(s)	296
Acc36I	ACCTGC	2 cut(s)	1168, 1615
AccB7I	CCANNNNNTGG	1 cut(s)	928
AccBSI	CCGCTC	1 cut(s)	1486
AccII	CGCG	1 cut(s)	1297
AciI	CCGC	9 cut(s)	54, 57, 75, 78, 81, 555, 1170, 1295, 1486
AclWI	GGATC	7 cut(s)	552, 565, 710, 935, 1066, 1095, 1599
AcsI	RAATTY	4 cut(s)	19, 701, 911, 1463
AcuI	CTGAAG	1 cut(s)	1437
AdeI	CACNNNGTG	1 cut(s)	928
AfaI	GTAC	4 cut(s)	320, 574, 951, 1048
AfiI	CCNNNNNNNGG	5 cut(s)	623, 928, 1397, 1539, 1682
AflIII	ACRYGT	1 cut(s)	474
AgsI	TTSAA	8 cut(s)	25, 241, 316, 406, 866, 1073, 1139, 1619
AjnI	CCWGG	4 cut(s)	195, 718, 846, 1357
AleI	CACNNNNGTG	1 cut(s)	1418
AloI	GAACNNNNNNTCC	2 cut(s)	619, 651
AluBI	AGCT	6 cut(s)	16, 409, 454, 635, 1409, 1654
AluI	AGCT	6 cut(s)	16, 409, 454, 635, 1409, 1654
Alw21I	GWGCWC	1 cut(s)	1690
Alw26I	GTCTC	3 cut(s)	219, 388, 614
AlwI	GGATC	7 cut(s)	552, 565, 710, 935, 1066, 1095, 1599
AlwNI	CAGNNNCTG	1 cut(s)	638
Ama87I	CYCGRG	1 cut(s)	617
AoxI	GGCC	2 cut(s)	1388, 1541
ApeKI	GCWGC	6 cut(s)	416, 656, 665, 668, 1299, 1451
ApoI	RAATTY	4 cut(s)	19, 701, 911, 1463
AspLEI	GCGC	4 cut(s)	297, 348, 492, 1299
AspS9I	GGNCC	3 cut(s)	150, 251, 1389
AsuC2I	CCSGG	2 cut(s)	1191, 1553
AsuHPI	GGTGA	4 cut(s)	238, 550, 1215, 1665
AvaI	CYCGRG	1 cut(s)	617
AvaII	GGWCC	2 cut(s)	150, 251
AxyI	CCTNAGG	1 cut(s)	639
BamHI	GGATCC	1 cut(s)	557
BauI	CACGAG	1 cut(s)	1545
BbsI	GAAGAC	2 cut(s)	873, 1101
Bbv12I	GWGCWC	1 cut(s)	1690
BbvCI	CCTCAGC	1 cut(s)	631
BbvI	GCAGC	6 cut(s)	428, 643, 652, 655, 1286, 1463
BccI	CCATC	3 cut(s)	92, 1315, 1676
BceAI	ACGGC	1 cut(s)	1615
BcgI	CGANNNNNNTGC	4 cut(s)	782, 816, 1637, 1671
BciT130I	CCWGG	4 cut(s)	197, 720, 848, 1359
BciVI	GTATCC	1 cut(s)	1025
BcnI	CCSGG	2 cut(s)	1191, 1553
BcoDI	GTCTC	3 cut(s)	219, 388, 614
BfaI	CTAG	2 cut(s)	552, 1044
BfuAI	ACCTGC	2 cut(s)	1168, 1615
BfuI	GTATCC	1 cut(s)	1025
BlpI	GCTNAGC	1 cut(s)	1380
BmcAI	AGTACT	1 cut(s)	574
Bme1390I	CCNGG	6 cut(s)	197, 720, 848, 1191, 1359, 1553
Bme18I	GGWCC	2 cut(s)	150, 251
BmeT110I	CYCGRG	1 cut(s)	617
BmgT120I	GGNCC	3 cut(s)	150, 251, 1389
BmiI	GGNNCC	5 cut(s)	6, 151, 252, 559, 628
BmrFI	CCNGG	6 cut(s)	197, 720, 848, 1191, 1359, 1553
BmsI	GCATC	5 cut(s)	223, 1263, 1363, 1619, 1667
BpiI	GAAGAC	2 cut(s)	873, 1101
Bpu10I	CCTNAGC	1 cut(s)	631
Bpu1102I	GCTNAGC	1 cut(s)	1380
BpuEI	CTTGAG	1 cut(s)	1092
BpuMI	CCSGG	2 cut(s)	1191, 1553
Bsa29I	ATCGAT	1 cut(s)	261
BsaBI	GATNNNNATC	2 cut(s)	261, 939
BsaI	GGTCTC	1 cut(s)	388
BsaJI	CCNNGG	4 cut(s)	846, 1190, 1385, 1552
BsaWI	WCCGGW	2 cut(s)	682, 1675
BsaXI	ACNNNNNCTCC	4 cut(s)	619, 649, 1531, 1561
Bsc4I	CCNNNNNNNGG	5 cut(s)	623, 928, 1397, 1539, 1682
Bse1I	ACTGG	3 cut(s)	46, 153, 745
Bse21I	CCTNAGG	1 cut(s)	639
Bse3DI	GCAATG	1 cut(s)	870
Bse8I	GATNNNNATC	2 cut(s)	261, 939
BseBI	CCWGG	4 cut(s)	197, 720, 848, 1359
BseCI	ATCGAT	1 cut(s)	261
BseDI	CCNNGG	4 cut(s)	846, 1190, 1385, 1552
BseGI	GGATG	5 cut(s)	595, 1120, 1326, 1354, 1639
BseJI	GATNNNNATC	2 cut(s)	261, 939
BseLI	CCNNNNNNNGG	5 cut(s)	623, 928, 1397, 1539, 1682
BseMI	GCAATG	1 cut(s)	870
BseMII	CTCAG	3 cut(s)	630, 645, 1394
BseNI	ACTGG	3 cut(s)	46, 153, 745
BseRI	GAGGAG	3 cut(s)	207, 1643, 1664
BseXI	GCAGC	6 cut(s)	428, 643, 652, 655, 1286, 1463
BsgI	GTGCAG	1 cut(s)	1101
Bsh1236I	CGCG	1 cut(s)	1297
BshFI	GGCC	2 cut(s)	1390, 1543
BshVI	ATCGAT	1 cut(s)	261
BsiHKAI	GWGCWC	1 cut(s)	1690
BsiHKCI	CYCGRG	1 cut(s)	617
BsiSI	CCGG	4 cut(s)	683, 1190, 1552, 1676
BslFI	GGGAC	5 cut(s)	44, 264, 344, 1569, 1655
BslI	CCNNNNNNNGG	5 cut(s)	623, 928, 1397, 1539, 1682
BsmAI	GTCTC	3 cut(s)	219, 388, 614
BsmFI	GGGAC	5 cut(s)	44, 264, 344, 1569, 1655
BsmI	GAATGC	3 cut(s)	247, 348, 1381
BsnI	GGCC	2 cut(s)	1390, 1543
Bso31I	GGTCTC	1 cut(s)	388
BsoBI	CYCGRG	1 cut(s)	617
Bsp1286I	GDGCHC	1 cut(s)	1690
Bsp143I	GATC	9 cut(s)	262, 532, 557, 715, 940, 1058, 1100, 1503, 1591
Bsp1720I	GCTNAGC	1 cut(s)	1380
Bsp19I	CCATGG	1 cut(s)	1385
BspACI	CCGC	9 cut(s)	54, 57, 75, 78, 81, 555, 1170, 1295, 1486
BspANI	GGCC	2 cut(s)	1390, 1543
BspCNI	CTCAG	3 cut(s)	631, 644, 1393
BspDI	ATCGAT	1 cut(s)	261
BspFNI	CGCG	1 cut(s)	1297
BspHI	TCATGA	1 cut(s)	360
BspLI	GGNNCC	5 cut(s)	6, 151, 252, 559, 628
BspMI	ACCTGC	2 cut(s)	1168, 1615
BspPI	GGATC	7 cut(s)	552, 565, 710, 935, 1066, 1095, 1599
BspTNI	GGTCTC	1 cut(s)	388
BsrBI	CCGCTC	1 cut(s)	1486
BsrDI	GCAATG	1 cut(s)	870
BsrI	ACTGG	3 cut(s)	46, 153, 745
BssECI	CCNNGG	4 cut(s)	846, 1190, 1385, 1552
BssMI	GATC	9 cut(s)	262, 532, 557, 715, 940, 1058, 1100, 1503, 1591
BssSI	CACGAG	1 cut(s)	1545
BssT1I	CCWWGG	1 cut(s)	1385
Bst2BI	CACGAG	1 cut(s)	1545
Bst2UI	CCWGG	4 cut(s)	197, 720, 848, 1359
Bst4CI	ACNGT	4 cut(s)	135, 391, 572, 1560
Bst6I	CTCTTC	2 cut(s)	156, 891
BstAPI	GCANNNNNTGC	2 cut(s)	242, 1255
BstC8I	GCNNGC	5 cut(s)	494, 504, 1116, 1352, 1656
BstDEI	CTNAG	3 cut(s)	631, 639, 1380
BstDSI	CCRYGG	1 cut(s)	1385
BstF5I	GGATG	5 cut(s)	595, 1120, 1326, 1354, 1639
BstFNI	CGCG	1 cut(s)	1297
BstHHI	GCGC	4 cut(s)	297, 348, 492, 1299
BstKTI	GATC	9 cut(s)	265, 535, 560, 718, 943, 1061, 1103, 1506, 1594
BstMAI	GTCTC	3 cut(s)	219, 388, 614
BstMBI	GATC	9 cut(s)	262, 532, 557, 715, 940, 1058, 1100, 1503, 1591
BstMWI	GCNNNNNNNGC	7 cut(s)	13, 54, 242, 662, 665, 1169, 1255
BstNI	CCWGG	4 cut(s)	197, 720, 848, 1359
BstNSI	RCATGY	4 cut(s)	478, 496, 1258, 1354
BstSCI	CCNGG	6 cut(s)	195, 718, 846, 1189, 1357, 1551
BstUI	CGCG	1 cut(s)	1297
BstV1I	GCAGC	6 cut(s)	428, 643, 652, 655, 1286, 1463
BstV2I	GAAGAC	2 cut(s)	873, 1101
BstX2I	RGATCY	3 cut(s)	557, 1100, 1591
BstXI	CCANNNNNNTGG	2 cut(s)	740, 953
BstYI	RGATCY	3 cut(s)	557, 1100, 1591
Bsu15I	ATCGAT	1 cut(s)	261
Bsu36I	CCTNAGG	1 cut(s)	639
BsuI	GTATCC	1 cut(s)	1025
BsuRI	GGCC	2 cut(s)	1390, 1543
BsuTUI	ATCGAT	1 cut(s)	261
BtgI	CCRYGG	1 cut(s)	1385
BtsCI	GGATG	5 cut(s)	595, 1120, 1326, 1354, 1639
BtsI	GCAGTG	1 cut(s)	55
BtsIMutI	CAGTG	3 cut(s)	55, 876, 1413
BveI	ACCTGC	2 cut(s)	1168, 1615
Cac8I	GCNNGC	5 cut(s)	494, 504, 1116, 1352, 1656
CaiI	CAGNNNCTG	1 cut(s)	638
CciI	TCATGA	1 cut(s)	360
CfoI	GCGC	4 cut(s)	297, 348, 492, 1299
Cfr13I	GGNCC	3 cut(s)	150, 251, 1389
ClaI	ATCGAT	1 cut(s)	261
CseI	GACGC	2 cut(s)	478, 602
CsiI	ACCWGGT	1 cut(s)	195
Csp6I	GTAC	4 cut(s)	319, 573, 950, 1047
CviQI	GTAC	4 cut(s)	319, 573, 950, 1047
DdeI	CTNAG	3 cut(s)	631, 639, 1380
DpnI	GATC	9 cut(s)	264, 534, 559, 717, 942, 1060, 1102, 1505, 1593
DpnII	GATC	9 cut(s)	262, 532, 557, 715, 940, 1058, 1100, 1503, 1591
DraIII	CACNNNGTG	1 cut(s)	928
Eam1104I	CTCTTC	2 cut(s)	156, 891
EarI	CTCTTC	2 cut(s)	156, 891
Eco130I	CCWWGG	1 cut(s)	1385
Eco147I	AGGCCT	1 cut(s)	1543
Eco31I	GGTCTC	1 cut(s)	388
Eco32I	GATATC	2 cut(s)	259, 543
Eco47I	GGWCC	2 cut(s)	150, 251
Eco57I	CTGAAG	1 cut(s)	1437
Eco81I	CCTNAGG	1 cut(s)	639
Eco88I	CYCGRG	1 cut(s)	617
EcoO109I	RGGNCCY	1 cut(s)	251
EcoRII	CCWGG	4 cut(s)	195, 718, 846, 1357
EcoRV	GATATC	2 cut(s)	259, 543
EcoT14I	CCWWGG	1 cut(s)	1385
EcoT22I	ATGCAT	3 cut(s)	238, 1352, 1356
ErhI	CCWWGG	1 cut(s)	1385
FalI	AAGNNNNNCTT	4 cut(s)	270, 302, 1393, 1425
FaqI	GGGAC	5 cut(s)	44, 264, 344, 1569, 1655
FauI	CCCGC	1 cut(s)	50
FokI	GGATG	5 cut(s)	602, 1107, 1333, 1341, 1646
FspBI	CTAG	2 cut(s)	552, 1044
FspI	TGCGCA	1 cut(s)	296
GlaI	GCGC	4 cut(s)	296, 347, 491, 1298
HaeIII	GGCC	2 cut(s)	1390, 1543
HapII	CCGG	4 cut(s)	683, 1190, 1552, 1676
HgaI	GACGC	2 cut(s)	478, 602
HhaI	GCGC	4 cut(s)	297, 348, 492, 1299
Hin6I	GCGC	4 cut(s)	295, 346, 490, 1297
HinP1I	GCGC	4 cut(s)	295, 346, 490, 1297
HincII	GTYRAC	3 cut(s)	283, 1364, 1438
HindII	GTYRAC	3 cut(s)	283, 1364, 1438
HindIII	AAGCTT	1 cut(s)	407
HinfI	GANTC	5 cut(s)	794, 1022, 1243, 1423, 1667
HpaII	CCGG	4 cut(s)	683, 1190, 1552, 1676
HphI	GGTGA	4 cut(s)	238, 550, 1215, 1665
Hpy166II	GTNNAC	5 cut(s)	283, 468, 773, 1364, 1438
Hpy188I	TCNGA	6 cut(s)	160, 459, 547, 589, 700, 1080
Hpy188III	TCNNGA	6 cut(s)	361, 617, 638, 688, 908, 1056
Hpy8I	GTNNAC	5 cut(s)	283, 468, 773, 1364, 1438
Hpy99I	CGWCG	1 cut(s)	1649
HpyAV	CCTTC	5 cut(s)	4, 296, 480, 814, 1437
HpyCH4III	ACNGT	4 cut(s)	135, 391, 572, 1560
HpyCH4V	TGCA	9 cut(s)	236, 245, 1118, 1249, 1258, 1350, 1354, 1610, 1658
HpyF10VI	GCNNNNNNNGC	7 cut(s)	13, 54, 242, 662, 665, 1169, 1255
HpyF3I	CTNAG	3 cut(s)	631, 639, 1380
HspAI	GCGC	4 cut(s)	295, 346, 490, 1297
Kzo9I	GATC	9 cut(s)	262, 532, 557, 715, 940, 1058, 1100, 1503, 1591
LmnI	GCTCC	8 cut(s)	4, 13, 114, 413, 640, 1483, 1651, 1693
Lsp1109I	GCAGC	6 cut(s)	428, 643, 652, 655, 1286, 1463
LweI	GCATC	5 cut(s)	223, 1263, 1363, 1619, 1667
MabI	ACCWGGT	1 cut(s)	195
MaeI	CTAG	2 cut(s)	552, 1044
MaeIII	GTNAC	2 cut(s)	728, 1221
MalI	GATC	9 cut(s)	264, 534, 559, 717, 942, 1060, 1102, 1505, 1593
MbiI	CCGCTC	1 cut(s)	1486
MboI	GATC	9 cut(s)	262, 532, 557, 715, 940, 1058, 1100, 1503, 1591
MflI	RGATCY	3 cut(s)	557, 1100, 1591
MhlI	GDGCHC	1 cut(s)	1690
MluCI	AATT	6 cut(s)	19, 308, 523, 701, 911, 1463
MlyI	GAGTC	1 cut(s)	803
MmeI	TCCRAC	1 cut(s)	1014
Mph1103I	ATGCAT	3 cut(s)	238, 1352, 1356
MseI	TTAA	6 cut(s)	341, 834, 1461, 1574, 1596, 1693
MslI	CAYNNNNRTG	3 cut(s)	957, 1314, 1418
MspI	CCGG	4 cut(s)	683, 1190, 1552, 1676
MspR9I	CCNGG	6 cut(s)	197, 720, 848, 1191, 1359, 1553
Mva1269I	GAATGC	3 cut(s)	247, 348, 1381
MvaI	CCWGG	4 cut(s)	197, 720, 848, 1359
MvnI	CGCG	1 cut(s)	1297
MwoI	GCNNNNNNNGC	7 cut(s)	13, 54, 242, 662, 665, 1169, 1255
NciI	CCSGG	2 cut(s)	1191, 1553
NcoI	CCATGG	1 cut(s)	1385
NdeII	GATC	9 cut(s)	262, 532, 557, 715, 940, 1058, 1100, 1503, 1591
NlaIV	GGNNCC	5 cut(s)	6, 151, 252, 559, 628
NmuCI	GTSAC	2 cut(s)	728, 1221
NsbI	TGCGCA	1 cut(s)	296
NsiI	ATGCAT	3 cut(s)	238, 1352, 1356
NspI	RCATGY	4 cut(s)	478, 496, 1258, 1354
OliI	CACNNNNGTG	1 cut(s)	1418
PaeI	GCATGC	2 cut(s)	496, 1354
PagI	TCATGA	1 cut(s)	360
PceI	AGGCCT	1 cut(s)	1543
PciI	ACATGT	1 cut(s)	474
PctI	GAATGC	3 cut(s)	247, 348, 1381
PfeI	GAWTC	4 cut(s)	1022, 1243, 1423, 1667
PflMI	CCANNNNNTGG	1 cut(s)	928
PfoI	TCCNGGA	1 cut(s)	718
PleI	GAGTC	1 cut(s)	802
PpsI	GAGTC	1 cut(s)	802
PpuMI	RGGWCCY	1 cut(s)	251
PscI	ACATGT	1 cut(s)	474
PsiI	TTATAA	1 cut(s)	521
Psp5II	RGGWCCY	1 cut(s)	251
Psp6I	CCWGG	4 cut(s)	195, 718, 846, 1357
PspGI	CCWGG	4 cut(s)	195, 718, 846, 1357
PspN4I	GGNNCC	5 cut(s)	6, 151, 252, 559, 628
PspPI	GGNCC	3 cut(s)	150, 251, 1389
PspPPI	RGGWCCY	1 cut(s)	251
PstNI	CAGNNNCTG	1 cut(s)	638
PsuI	RGATCY	3 cut(s)	557, 1100, 1591
RsaI	GTAC	4 cut(s)	320, 574, 951, 1048
RsaNI	GTAC	4 cut(s)	319, 573, 950, 1047
RseI	CAYNNNNRTG	3 cut(s)	957, 1314, 1418
SaqAI	TTAA	6 cut(s)	341, 834, 1461, 1574, 1596, 1693
Sau3AI	GATC	9 cut(s)	262, 532, 557, 715, 940, 1058, 1100, 1503, 1591
Sau96I	GGNCC	3 cut(s)	150, 251, 1389
ScaI	AGTACT	1 cut(s)	574
SchI	GAGTC	1 cut(s)	803
ScrFI	CCNGG	6 cut(s)	197, 720, 848, 1191, 1359, 1553
SduI	GDGCHC	1 cut(s)	1690
SexAI	ACCWGGT	1 cut(s)	195
SfaNI	GCATC	5 cut(s)	223, 1263, 1363, 1619, 1667
SinI	GGWCC	2 cut(s)	150, 251
SmiMI	CAYNNNNRTG	3 cut(s)	957, 1314, 1418
SmlI	CTYRAG	1 cut(s)	1107
SmoI	CTYRAG	1 cut(s)	1107
SphI	GCATGC	2 cut(s)	496, 1354
Sse9I	AATT	6 cut(s)	19, 308, 523, 701, 911, 1463
SseBI	AGGCCT	1 cut(s)	1543
SsiI	CCGC	9 cut(s)	54, 57, 75, 78, 81, 555, 1170, 1295, 1486
SspI	AATATT	1 cut(s)	1181
SspMI	CTAG	2 cut(s)	552, 1044
StuI	AGGCCT	1 cut(s)	1543
StyD4I	CCNGG	6 cut(s)	195, 718, 846, 1189, 1357, 1551
StyI	CCWWGG	1 cut(s)	1385
TaaI	ACNGT	4 cut(s)	135, 391, 572, 1560
TaqI	TCGA	4 cut(s)	108, 220, 261, 1519
TasI	AATT	6 cut(s)	19, 308, 523, 701, 911, 1463
TatI	WGTACW	3 cut(s)	318, 572, 949
TauI	GCSGC	4 cut(s)	57, 78, 81, 1172
TfiI	GAWTC	4 cut(s)	1022, 1243, 1423, 1667
Tru1I	TTAA	6 cut(s)	341, 834, 1461, 1574, 1596, 1693
Tru9I	TTAA	6 cut(s)	341, 834, 1461, 1574, 1596, 1693
TscAI	CASTG	3 cut(s)	55, 883, 1420
TseFI	GTSAC	2 cut(s)	728, 1221
TseI	GCWGC	6 cut(s)	416, 656, 665, 668, 1299, 1451
Tsp45I	GTSAC	2 cut(s)	728, 1221
TspDTI	ATGAA	5 cut(s)	51, 94, 320, 1146, 1650
TspGWI	ACGGA	1 cut(s)	196
TspRI	CASTG	3 cut(s)	55, 883, 1420
Van91I	CCANNNNNTGG	1 cut(s)	928
VpaK11BI	GGWCC	2 cut(s)	150, 251
XapI	RAATTY	4 cut(s)	19, 701, 911, 1463
XceI	RCATGY	4 cut(s)	478, 496, 1258, 1354
XspI	CTAG	2 cut(s)	552, 1044
ZrmI	AGTACT	1 cut(s)	574
Zsp2I	ATGCAT	3 cut(s)	238, 1352, 1356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.