Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0002967

Physical Location & Seq

Genomic Coordinates Reverse (-)

5064148 .. 5065483

Gene Structure 1336 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1054800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGTCGAGTGGAAAACTCTCAGATGCATTCAAATCCCACCCTATCTTCCTCCACAAGCAACACCCAGACGACTTCAGCTCATTACAGGAATTGCCAGAGTCGTATGCATGGACGTCACAGCTTAACGAATATCCGTCTTTCGAATCATTATCTTGCTCAGAGTCTGTGCCGGTAATCGATCTCGCCGACCCAAATGTCGTTGAACTCGTAGGACATGCATGCAAGACTTGGGGCATGTTCCAAGCCACAAACCATGGCGTCCCCCAAAAACTTGTTGATGACATGGAATCTACTGTTCGAAGCCTTTTCTCTCTGCCACCCCAACAAAAGCTCAAAGCAGCTCGATTGCCCGATGGCATTTCCGGTTACGGTTTCCATCGCATATCTGCCTTTTGTGAAAAGCTAATGTGGTCTGAGAGCTTCACTATCGTTGGCTCAGCACTTCAACATTTTTGCCAACTTTGGCCCCAAGATCACACCAAGTTCTGTTGTGTTACTGAAGAATATGAGAAAGAGATGAAAAGGCTTGCAGCTAGGTTGATGTGGCTAATGCTTGGCTCATTAGACATATCCAAGGAAGACATCAAATGGGCGGGTCCAAGAGGTGAATTTGAAGATGCATCTGCAGCCTTACAATTGAACTCATACCCCGCTTGTCCGGATCCAGATCGGACCATGGGTCTTGCCCAACATACTGATTCAAACCTCCTGTCAATTATCCACCAAAGCGCTAAGGCCAGTGGCTTGCAAGTTTTCCAAGAAGGGAAGGACGAGGGCCGGGGGTGGCTTATGGTTCCACCGGTCCCGGGGGCTTTTGTGATCAATGTGGGTGACTTCATTCACATATTATCAAACGGATTGTACCAGACCCCCCTTCACCGGGCAGTGGTGAACCGGAGCCAGCACCGTGTATCAGTTGCTTACTTATATGGTCCTCCGGAGAATGCTCAGGTCTCTCCCCTCTCGAAATTACTCGGCCCAAGTTGCCCTCCGCTGTACGCGCCCGTCACTTGGAATGAGTACCTTGGAACCAAGGCAAAGCTTAACAACAAGGCACTTCAATCACTCCGACTGAATGCTCCTCTGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009987 GO:0010033 GO:0010114 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0016707 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01110 map00904 map01110

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

364

Amino Acids

40.35

Weight (kDa)

6.11

Isoelectric Point (pI)

46.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	56 - 146	2e-20	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	209 - 312	2.1e-29	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002967)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15550 AT1G80340
fragaria_vesca	FvH4_6g30780
malus_domestica	MD02G1263100.v1.1 MD02G1263200.v1.1 MD07G1054800.v1.1 MD09G1217400.v1.1 MD17G1199100.v1.1
prunus_persica	Prupe.2G061700_v2.0.a1 Prupe.3G075600_v2.0.a1
pyrus_communis	pycom07g04260 pycom09g13440 pycom17g20380
rosa_chinensis	RchiOBHm_Chr2g0137231 RchiOBHm_Chr7g0236321
rosa_laevigata	RLG00000001086 RLG00000019636
rosa_multiflora	Rmu_co8273963.1_g000001 Rmu_sc0000894.1_g000034 Rmu_sc0001441.1_g000006 Rmu_sc0009898.1_g000005 Rmu_sc0029248.1_g000002
rosa_roxburghii	Rroxscaffold_2G00107310 Rroxscaffold_3G00225230
rosa_rugosa	Rorug02G0339700 Rorug07G0295900 Rorug07G0296000
rosa_samantha	Rh2AG388500 Rh2BG396100 Rh2CG375900 Rh2DG411200 Rh7AG450800 Rh7BG422300 Rh7CG470400 Rh7CG470500 Rh7DG439800
rosa_wichuraiana	Rw2G031830 Rw7G037480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	194
AatII	GACGTC	1 cut(s)	116
AccII	CGCG	1 cut(s)	1001
AccIII	TCCGGA	2 cut(s)	658, 937
AciI	CCGC	3 cut(s)	593, 651, 992
AclWI	GGATC	2 cut(s)	656, 669
AcsI	RAATTY	1 cut(s)	608
AcuI	CTGAAG	2 cut(s)	58, 519
AcyI	GRCGYC	2 cut(s)	113, 258
AfaI	GTAC	3 cut(s)	863, 998, 1022
AfeI	AGCGCT	1 cut(s)	730
AfiI	CCNNNNNNNGG	5 cut(s)	806, 879, 880, 886, 1011
AgeI	ACCGGT	1 cut(s)	799
AgsI	TTSAA	7 cut(s)	31, 203, 446, 614, 640, 702, 1061
AhdI	GACNNNNNGTC	1 cut(s)	678
AluBI	AGCT	8 cut(s)	78, 121, 331, 341, 403, 420, 533, 1042
AluI	AGCT	8 cut(s)	78, 121, 331, 341, 403, 420, 533, 1042
Alw26I	GTCTC	1 cut(s)	958
AlwI	GGATC	2 cut(s)	656, 669
AlwNI	CAGNNNCTG	1 cut(s)	164
Ama87I	CYCGRG	1 cut(s)	805
Aor13HI	TCCGGA	2 cut(s)	658, 937
Aor51HI	AGCGCT	1 cut(s)	730
AoxI	GGCC	4 cut(s)	464, 735, 775, 976
ApeKI	GCWGC	3 cut(s)	338, 530, 626
ApoI	RAATTY	1 cut(s)	608
ArsI	GACNNNNNNTTYG	2 cut(s)	695, 727
AsiGI	ACCGGT	1 cut(s)	799
AspLEI	GCGC	2 cut(s)	731, 1003
AspS9I	GGNCC	7 cut(s)	465, 596, 672, 775, 802, 932, 977
AsuC2I	CCSGG	4 cut(s)	779, 806, 807, 881
AsuHPI	GGTGA	4 cut(s)	617, 842, 869, 901
AsuII	TTCGAA	2 cut(s)	141, 298
AvaI	CYCGRG	1 cut(s)	805
AvaII	GGWCC	4 cut(s)	596, 672, 802, 932
BamHI	GGATCC	1 cut(s)	661
BbsI	GAAGAC	1 cut(s)	585
BbvI	GCAGC	3 cut(s)	350, 542, 638
BccI	CCATC	2 cut(s)	347, 384
BclI	TGATCA	1 cut(s)	819
BcnI	CCSGG	4 cut(s)	779, 806, 807, 881
BcoDI	GTCTC	1 cut(s)	958
BfaI	CTAG	1 cut(s)	534
BfmI	CTRYAG	1 cut(s)	624
BfoI	RGCGCY	1 cut(s)	732
BisI	GCNGC	3 cut(s)	339, 531, 627
BlpI	GCTNAGC	1 cut(s)	436
BlsI	GCNGC	3 cut(s)	340, 532, 628
Bme1390I	CCNGG	4 cut(s)	779, 806, 807, 881
Bme18I	GGWCC	4 cut(s)	596, 672, 802, 932
BmeRI	GACNNNNNGTC	1 cut(s)	678
BmeT110I	CYCGRG	1 cut(s)	805
BmgT120I	GGNCC	7 cut(s)	465, 596, 672, 775, 802, 932, 977
BmiI	GGNNCC	7 cut(s)	467, 597, 663, 795, 804, 899, 1030
BmrFI	CCNGG	4 cut(s)	779, 806, 807, 881
BmsI	GCATC	3 cut(s)	13, 607, 629
BpiI	GAAGAC	1 cut(s)	585
Bpu10I	CCTNAGC	2 cut(s)	732, 948
Bpu1102I	GCTNAGC	1 cut(s)	436
Bpu14I	TTCGAA	2 cut(s)	141, 298
BpuMI	CCSGG	4 cut(s)	779, 806, 807, 881
Bsa29I	ATCGAT	1 cut(s)	177
BsaBI	GATNNNNATC	1 cut(s)	666
BsaHI	GRCGYC	2 cut(s)	113, 258
BsaI	GGTCTC	1 cut(s)	958
BsaJI	CCNNGG	8 cut(s)	253, 573, 675, 778, 805, 806, 1024, 1032
BsaWI	WCCGGW	5 cut(s)	362, 658, 799, 894, 937
Bsc4I	CCNNNNNNNGG	5 cut(s)	806, 879, 880, 886, 1011
Bse118I	RCCGGY	2 cut(s)	169, 799
Bse1I	ACTGG	1 cut(s)	738
Bse8I	GATNNNNATC	1 cut(s)	666
BseAI	TCCGGA	2 cut(s)	658, 937
BseCI	ATCGAT	1 cut(s)	177
BseDI	CCNNGG	8 cut(s)	253, 573, 675, 778, 805, 806, 1024, 1032
BseJI	GATNNNNATC	1 cut(s)	666
BseLI	CCNNNNNNNGG	5 cut(s)	806, 879, 880, 886, 1011
BseMII	CTCAG	5 cut(s)	33, 171, 405, 450, 962
BseNI	ACTGG	1 cut(s)	738
BseRI	GAGGAG	1 cut(s)	1071
BseXI	GCAGC	3 cut(s)	350, 542, 638
Bsh1236I	CGCG	1 cut(s)	1001
BshFI	GGCC	4 cut(s)	466, 737, 777, 978
BshTI	ACCGGT	1 cut(s)	799
BshVI	ATCGAT	1 cut(s)	177
BsiHKCI	CYCGRG	1 cut(s)	805
BsiSI	CCGG	9 cut(s)	170, 363, 659, 778, 800, 806, 880, 895, 938
BslFI	GGGAC	2 cut(s)	245, 788
BslI	CCNNNNNNNGG	5 cut(s)	806, 879, 880, 886, 1011
BsmAI	GTCTC	1 cut(s)	958
BsmFI	GGGAC	2 cut(s)	245, 788
BsmI	GAATGC	3 cut(s)	26, 949, 1081
BsnI	GGCC	4 cut(s)	466, 737, 777, 978
Bso31I	GGTCTC	1 cut(s)	958
BsoBI	CYCGRG	1 cut(s)	805
Bsp119I	TTCGAA	2 cut(s)	141, 298
Bsp13I	TCCGGA	2 cut(s)	658, 937
Bsp143I	GATC	5 cut(s)	178, 472, 661, 667, 819
Bsp1720I	GCTNAGC	1 cut(s)	436
Bsp19I	CCATGG	2 cut(s)	253, 675
BspACI	CCGC	3 cut(s)	593, 651, 992
BspANI	GGCC	4 cut(s)	466, 737, 777, 978
BspCNI	CTCAG	5 cut(s)	32, 170, 406, 449, 961
BspDI	ATCGAT	1 cut(s)	177
BspEI	TCCGGA	2 cut(s)	658, 937
BspFNI	CGCG	1 cut(s)	1001
BspLI	GGNNCC	7 cut(s)	467, 597, 663, 795, 804, 899, 1030
BspMAI	CTGCAG	1 cut(s)	628
BspPI	GGATC	2 cut(s)	656, 669
BspT104I	TTCGAA	2 cut(s)	141, 298
BspTNI	GGTCTC	1 cut(s)	958
BsrFI	RCCGGY	2 cut(s)	169, 799
BsrI	ACTGG	1 cut(s)	738
BssAI	RCCGGY	2 cut(s)	169, 799
BssECI	CCNNGG	8 cut(s)	253, 573, 675, 778, 805, 806, 1024, 1032
BssMI	GATC	5 cut(s)	178, 472, 661, 667, 819
BssNI	GRCGYC	2 cut(s)	113, 258
BssT1I	CCWWGG	5 cut(s)	253, 573, 675, 1024, 1032
Bst4CI	ACNGT	3 cut(s)	295, 371, 908
BstACI	GRCGYC	2 cut(s)	113, 258
BstBI	TTCGAA	2 cut(s)	141, 298
BstC8I	GCNNGC	4 cut(s)	220, 528, 746, 902
BstDEI	CTNAG	6 cut(s)	19, 157, 414, 436, 732, 948
BstDSI	CCRYGG	2 cut(s)	253, 675
BstFNI	CGCG	1 cut(s)	1001
BstH2I	RGCGCY	1 cut(s)	732
BstHHI	GCGC	2 cut(s)	731, 1003
BstKTI	GATC	5 cut(s)	181, 475, 664, 670, 822
BstMAI	GTCTC	1 cut(s)	958
BstMBI	GATC	5 cut(s)	178, 472, 661, 667, 819
BstMWI	GCNNNNNNNGC	3 cut(s)	626, 984, 1000
BstNSI	RCATGY	3 cut(s)	218, 222, 238
BstSCI	CCNGG	4 cut(s)	777, 804, 805, 879
BstSFI	CTRYAG	1 cut(s)	624
BstUI	CGCG	1 cut(s)	1001
BstV1I	GCAGC	3 cut(s)	350, 542, 638
BstV2I	GAAGAC	1 cut(s)	585
BstX2I	RGATCY	1 cut(s)	661
BstYI	RGATCY	1 cut(s)	661
Bsu15I	ATCGAT	1 cut(s)	177
BsuRI	GGCC	4 cut(s)	466, 737, 777, 978
BsuTUI	ATCGAT	1 cut(s)	177
BtgI	CCRYGG	2 cut(s)	253, 675
BtgZI	GCGATG	1 cut(s)	362
BtsI	GCAGTG	1 cut(s)	891
BtsIMutI	CAGTG	2 cut(s)	745, 891
Cac8I	GCNNGC	4 cut(s)	220, 528, 746, 902
CaiI	CAGNNNCTG	1 cut(s)	164
CfoI	GCGC	2 cut(s)	731, 1003
Cfr10I	RCCGGY	2 cut(s)	169, 799
Cfr13I	GGNCC	7 cut(s)	465, 596, 672, 775, 802, 932, 977
Cfr9I	CCCGGG	1 cut(s)	805
ClaI	ATCGAT	1 cut(s)	177
CseI	GACGC	1 cut(s)	247
Csp6I	GTAC	3 cut(s)	862, 997, 1021
CspAI	ACCGGT	1 cut(s)	799
CviAII	CATG	7 cut(s)	108, 215, 219, 235, 254, 283, 676
CviQI	GTAC	3 cut(s)	862, 997, 1021
DdeI	CTNAG	6 cut(s)	19, 157, 414, 436, 732, 948
DpnI	GATC	5 cut(s)	180, 474, 663, 669, 821
DpnII	GATC	5 cut(s)	178, 472, 661, 667, 819
DrdI	GACNNNNNNGTC	1 cut(s)	194
DriI	GACNNNNNGTC	1 cut(s)	678
DseDI	GACNNNNNNGTC	1 cut(s)	194
Eam1105I	GACNNNNNGTC	1 cut(s)	678
Eco130I	CCWWGG	5 cut(s)	253, 573, 675, 1024, 1032
Eco31I	GGTCTC	1 cut(s)	958
Eco47I	GGWCC	4 cut(s)	596, 672, 802, 932
Eco47III	AGCGCT	1 cut(s)	730
Eco57I	CTGAAG	2 cut(s)	58, 519
Eco88I	CYCGRG	1 cut(s)	805
EcoT14I	CCWWGG	5 cut(s)	253, 573, 675, 1024, 1032
EcoT22I	ATGCAT	4 cut(s)	28, 109, 220, 622
ErhI	CCWWGG	5 cut(s)	253, 573, 675, 1024, 1032
FaeI	CATG	7 cut(s)	111, 218, 222, 238, 257, 286, 679
FaqI	GGGAC	2 cut(s)	245, 788
FatI	CATG	7 cut(s)	107, 214, 218, 234, 253, 282, 675
FauI	CCCGC	2 cut(s)	586, 658
FbaI	TGATCA	1 cut(s)	819
Fnu4HI	GCNGC	3 cut(s)	339, 531, 627
Fsp4HI	GCNGC	3 cut(s)	339, 531, 627
FspBI	CTAG	1 cut(s)	534
GlaI	GCGC	2 cut(s)	730, 1002
GluI	GCNGC	3 cut(s)	339, 531, 627
HaeII	RGCGCY	1 cut(s)	732
HaeIII	GGCC	4 cut(s)	466, 737, 777, 978
HapII	CCGG	9 cut(s)	170, 363, 659, 778, 800, 806, 880, 895, 938
HgaI	GACGC	1 cut(s)	247
HhaI	GCGC	2 cut(s)	731, 1003
Hin1I	GRCGYC	2 cut(s)	113, 258
Hin1II	CATG	7 cut(s)	111, 218, 222, 238, 257, 286, 679
Hin6I	GCGC	2 cut(s)	729, 1001
HinP1I	GCGC	2 cut(s)	729, 1001
HindIII	AAGCTT	1 cut(s)	1040
HinfI	GANTC	5 cut(s)	98, 143, 161, 287, 698
HpaII	CCGG	9 cut(s)	170, 363, 659, 778, 800, 806, 880, 895, 938
HphI	GGTGA	4 cut(s)	617, 842, 869, 901
Hpy166II	GTNNAC	1 cut(s)	892
Hpy188I	TCNGA	6 cut(s)	22, 160, 415, 672, 1070, 1086
Hpy188III	TCNNGA	4 cut(s)	659, 665, 938, 964
Hpy8I	GTNNAC	1 cut(s)	892
HpyAV	CCTTC	3 cut(s)	755, 760, 884
HpyCH4III	ACNGT	3 cut(s)	295, 371, 908
HpyCH4IV	ACGT	1 cut(s)	113
HpyCH4V	TGCA	8 cut(s)	26, 107, 218, 222, 530, 620, 626, 748
HpyF10VI	GCNNNNNNNGC	3 cut(s)	626, 984, 1000
HpyF3I	CTNAG	6 cut(s)	19, 157, 414, 436, 732, 948
HpySE526I	ACGT	1 cut(s)	113
Hsp92I	GRCGYC	2 cut(s)	113, 258
Hsp92II	CATG	7 cut(s)	111, 218, 222, 238, 257, 286, 679
HspAI	GCGC	2 cut(s)	729, 1001
Kpn2I	TCCGGA	2 cut(s)	658, 937
Ksp22I	TGATCA	1 cut(s)	819
Kzo9I	GATC	5 cut(s)	178, 472, 661, 667, 819
LmnI	GCTCC	2 cut(s)	897, 1084
Lsp1109I	GCAGC	3 cut(s)	350, 542, 638
LweI	GCATC	3 cut(s)	13, 607, 629
MaeI	CTAG	1 cut(s)	534
MaeII	ACGT	1 cut(s)	113
MaeIII	GTNAC	5 cut(s)	114, 365, 493, 830, 1006
MalI	GATC	5 cut(s)	180, 474, 663, 669, 821
MboI	GATC	5 cut(s)	178, 472, 661, 667, 819
MboII	GAAGA	4 cut(s)	37, 512, 590, 626
MfeI	CAATTG	1 cut(s)	635
MflI	RGATCY	1 cut(s)	661
MluCI	AATT	6 cut(s)	89, 608, 635, 714, 968, 1087
MlyI	GAGTC	2 cut(s)	107, 170
MnlI	CCTC	8 cut(s)	59, 596, 716, 766, 945, 971, 999, 1092
Mph1103I	ATGCAT	4 cut(s)	28, 109, 220, 622
MroI	TCCGGA	2 cut(s)	658, 937
MseI	TTAA	2 cut(s)	123, 1044
MspA1I	CMGCKG	1 cut(s)	994
MspI	CCGG	9 cut(s)	170, 363, 659, 778, 800, 806, 880, 895, 938
MspR9I	CCNGG	4 cut(s)	779, 806, 807, 881
MunI	CAATTG	1 cut(s)	635
Mva1269I	GAATGC	3 cut(s)	26, 949, 1081
MvnI	CGCG	1 cut(s)	1001
MwoI	GCNNNNNNNGC	3 cut(s)	626, 984, 1000
NciI	CCSGG	4 cut(s)	779, 806, 807, 881
NcoI	CCATGG	2 cut(s)	253, 675
NdeII	GATC	5 cut(s)	178, 472, 661, 667, 819
NlaIII	CATG	7 cut(s)	111, 218, 222, 238, 257, 286, 679
NlaIV	GGNNCC	7 cut(s)	467, 597, 663, 795, 804, 899, 1030
NmeAIII	GCCGAG	1 cut(s)	954
NmuCI	GTSAC	3 cut(s)	114, 830, 1006
NsiI	ATGCAT	4 cut(s)	28, 109, 220, 622
NspI	RCATGY	3 cut(s)	218, 222, 238
NspV	TTCGAA	2 cut(s)	141, 298
PaeI	GCATGC	1 cut(s)	222
PcsI	WCGNNNNNNNCGW	2 cut(s)	183, 204
PctI	GAATGC	3 cut(s)	26, 949, 1081
PfeI	GAWTC	3 cut(s)	143, 287, 698
PinAI	ACCGGT	1 cut(s)	799
PkrI	GCNGC	3 cut(s)	340, 532, 628
PleI	GAGTC	2 cut(s)	106, 169
PpsI	GAGTC	2 cut(s)	106, 169
PspN4I	GGNNCC	7 cut(s)	467, 597, 663, 795, 804, 899, 1030
PspPI	GGNCC	7 cut(s)	465, 596, 672, 775, 802, 932, 977
PstI	CTGCAG	1 cut(s)	628
PstNI	CAGNNNCTG	1 cut(s)	164
PsuI	RGATCY	1 cut(s)	661
RsaI	GTAC	3 cut(s)	863, 998, 1022
RsaNI	GTAC	3 cut(s)	862, 997, 1021
SaqAI	TTAA	2 cut(s)	123, 1044
SatI	GCNGC	3 cut(s)	339, 531, 627
Sau3AI	GATC	5 cut(s)	178, 472, 661, 667, 819
Sau96I	GGNCC	7 cut(s)	465, 596, 672, 775, 802, 932, 977
SchI	GAGTC	2 cut(s)	107, 170
ScrFI	CCNGG	4 cut(s)	779, 806, 807, 881
SfaNI	GCATC	3 cut(s)	13, 607, 629
SfcI	CTRYAG	1 cut(s)	624
SfuI	TTCGAA	2 cut(s)	141, 298
SinI	GGWCC	4 cut(s)	596, 672, 802, 932
SmaI	CCCGGG	1 cut(s)	807
SphI	GCATGC	1 cut(s)	222
Sse9I	AATT	6 cut(s)	89, 608, 635, 714, 968, 1087
SsiI	CCGC	3 cut(s)	593, 651, 992
SspMI	CTAG	1 cut(s)	534
StyD4I	CCNGG	4 cut(s)	777, 804, 805, 879
StyI	CCWWGG	5 cut(s)	253, 573, 675, 1024, 1032
TaaI	ACNGT	3 cut(s)	295, 371, 908
TaiI	ACGT	1 cut(s)	116
TaqI	TCGA	6 cut(s)	5, 141, 177, 298, 343, 965
TasI	AATT	6 cut(s)	89, 608, 635, 714, 968, 1087
TfiI	GAWTC	3 cut(s)	143, 287, 698
Tru1I	TTAA	2 cut(s)	123, 1044
Tru9I	TTAA	2 cut(s)	123, 1044
TscAI	CASTG	2 cut(s)	745, 891
TseFI	GTSAC	3 cut(s)	114, 830, 1006
TseI	GCWGC	3 cut(s)	338, 530, 626
Tsp45I	GTSAC	3 cut(s)	114, 830, 1006
TspDTI	ATGAA	2 cut(s)	533, 826
TspGWI	ACGGA	2 cut(s)	123, 870
TspMI	CCCGGG	1 cut(s)	805
TspRI	CASTG	2 cut(s)	745, 891
VpaK11BI	GGWCC	4 cut(s)	596, 672, 802, 932
XapI	RAATTY	1 cut(s)	608
XceI	RCATGY	3 cut(s)	218, 222, 238
XmaI	CCCGGG	1 cut(s)	805
XspI	CTAG	1 cut(s)	534
ZraI	GACGTC	1 cut(s)	114
Zsp2I	ATGCAT	4 cut(s)	28, 109, 220, 622

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.