Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002967

Physical Location & Seq

Genomic Coordinates Reverse (-)

54677403 .. 54678798

Gene Structure 1396 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000019636

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1080 bp

ATGCCTGTAAGAATCTCCGATGCCTTTCAATCCCACCCCCATAAACACTACCCTGCAGACTTCAACTCACTGCACGAGTTACCGGACTCATATGCATGGACTCATCAACTCGACGAGTACCCATCTCTGGACTCGTCATTCGGCTCAGAGCTGTGTGTTCCGATCATCGATCTAACTCACCAAAATGCCCTTAAACTCATAGGACATGCATGTAAAACATGGGGGGTGTTCCAAGTCACAAACCATGGAATCCCAGAGAAGCTTCTTCATGACATTGAATCAACTTGTCGAAGCCTGTTCTCTCTGCCAGTCCAGCAGAAGCTCAAAGCCGCTCGACCGGCGGATGGCATTTCTGGTTATGGAATCCATCGTATATCTTCGTTTTTCCAAAAACTCATGTGGTCGGAGGGCTTTACTATTGCTGGCTCACCACTCGAACATTTTCGACAACTCTGGCCCCAAGATTACAACAAATTCTGTTGTTTGGTAGAAGAATATGAGAAAGAGATGAAAGGGCTAGCGGGGAAGCTGATGTGGTTAATATTTGGATCGCTGGGCATTCCGAAGGAAGACGTAATTTGGGCCGGCCCAAAAGGTGACTTTGAAGATGCATCCAATGCCATACAATTTAATTCTTACCCTGCATGTCCGGATCCCAATCGTGCCATGGGTTTGGCCGAACATACGGATTCAACCCTCCTGTCAATCATCCACCAAACCAACATAAGTGGCTTGCAAGTTCTTCATGAGGGCGCCGGGTGGCTCACGGTTCCGCCAGTCGCGGGAGCTTTGGTGGTCAACATTGGGGACCTTACTCATATCTTGTCCAACGGGGTGTACCACAATGTCCTTCACCGGGCAATTGTGAACCGGAGTCAACAACGTCTATCCATTGCTTACCTATATGGGCCACCAGCTAGTGTCCAAATCTCGCCTCAGTCGAAAGTAGTCACCCCAAGTCACCCTCCCCTTTATCGGCCAATCACTTGGAAGGAGTACCTTGCAATCAAAGGAAGACTTTACAACCAGACCCTTTCATCAATTAAGCTTTATGATCCTCTAAATGACCAAATGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009987 GO:0010033 GO:0010114 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0016707 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01110 map00904 map01110

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

360

Amino Acids

40.19

Weight (kDa)

6.4

Isoelectric Point (pI)

42.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	53 - 154	5.5e-22	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	208 - 304	1.3e-29	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002967)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15550 AT1G80340
fragaria_vesca	FvH4_6g30780
malus_domestica	MD02G1263100.v1.1 MD02G1263200.v1.1 MD07G1054800.v1.1 MD09G1217400.v1.1 MD17G1199100.v1.1
prunus_persica	Prupe.2G061700_v2.0.a1 Prupe.3G075600_v2.0.a1
pyrus_communis	pycom07g04260 pycom09g13440 pycom17g20380
rosa_chinensis	RchiOBHm_Chr2g0137231 RchiOBHm_Chr7g0236321
rosa_laevigata	RLG00000001086 RLG00000019636
rosa_multiflora	Rmu_co8273963.1_g000001 Rmu_sc0000894.1_g000034 Rmu_sc0001441.1_g000006 Rmu_sc0009898.1_g000005 Rmu_sc0029248.1_g000002
rosa_roxburghii	Rroxscaffold_2G00107310 Rroxscaffold_3G00225230
rosa_rugosa	Rorug02G0339700 Rorug07G0295900 Rorug07G0296000
rosa_samantha	Rh2AG388500 Rh2BG396100 Rh2CG375900 Rh2DG411200 Rh7AG450800 Rh7BG422300 Rh7CG470400 Rh7CG470500 Rh7DG439800
rosa_wichuraiana	Rw2G031830 Rw7G037480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	752
AccBSI	CCGCTC	1 cut(s)	332
AccII	CGCG	1 cut(s)	782
AccIII	TCCGGA	1 cut(s)	649
AciI	CCGC	5 cut(s)	330, 341, 521, 773, 782
AclWI	GGATC	4 cut(s)	556, 647, 660, 1049
AcoI	YGGCCR	2 cut(s)	675, 977
AcsI	RAATTY	1 cut(s)	473
AcyI	GRCGYC	1 cut(s)	753
AfaI	GTAC	3 cut(s)	119, 839, 998
AfiI	CCNNNNNNNGG	5 cut(s)	127, 344, 782, 856, 975
AgsI	TTSAA	5 cut(s)	29, 64, 278, 605, 693
AluBI	AGCT	7 cut(s)	151, 262, 322, 529, 788, 917, 1048
AluI	AGCT	7 cut(s)	151, 262, 322, 529, 788, 917, 1048
AlwI	GGATC	4 cut(s)	556, 647, 660, 1049
Aor13HI	TCCGGA	1 cut(s)	649
AoxI	GGCC	6 cut(s)	455, 582, 586, 675, 908, 977
ApoI	RAATTY	1 cut(s)	473
ArsI	GACNNNNNNTTYG	2 cut(s)	122, 154
Asp700I	GAANNNNTTC	1 cut(s)	441
AspLEI	GCGC	1 cut(s)	755
AspS9I	GGNCC	5 cut(s)	456, 582, 587, 808, 908
AsuC2I	CCSGG	2 cut(s)	757, 857
AsuHPI	GGTGA	6 cut(s)	170, 420, 608, 845, 943, 953
AsuNHI	GCTAGC	1 cut(s)	517
AvaII	GGWCC	1 cut(s)	808
BamHI	GGATCC	1 cut(s)	652
BanI	GGYRCC	1 cut(s)	752
BauI	CACGAG	1 cut(s)	74
BbsI	GAAGAC	2 cut(s)	576, 1021
BccI	CCATC	3 cut(s)	130, 338, 375
BcnI	CCSGG	2 cut(s)	757, 857
BfaI	CTAG	2 cut(s)	518, 918
BfmI	CTRYAG	1 cut(s)	54
BfoI	RGCGCY	1 cut(s)	756
BisI	GCNGC	1 cut(s)	330
BlsI	GCNGC	1 cut(s)	331
Bme1390I	CCNGG	2 cut(s)	757, 857
Bme18I	GGWCC	1 cut(s)	808
BmgT120I	GGNCC	5 cut(s)	456, 582, 587, 808, 908
BmiI	GGNNCC	5 cut(s)	458, 654, 754, 771, 809
BmrFI	CCNGG	2 cut(s)	757, 857
BmsI	GCATC	3 cut(s)	10, 598, 620
BmtI	GCTAGC	1 cut(s)	521
BpiI	GAAGAC	2 cut(s)	576, 1021
BpuMI	CCSGG	2 cut(s)	757, 857
Bsa29I	ATCGAT	1 cut(s)	168
BsaBI	GATNNNNATC	1 cut(s)	657
BsaHI	GRCGYC	1 cut(s)	753
BsaJI	CCNNGG	2 cut(s)	244, 666
BsaWI	WCCGGW	3 cut(s)	82, 649, 870
BsaXI	ACNNNNNCTCC	2 cut(s)	777, 807
Bsc4I	CCNNNNNNNGG	5 cut(s)	127, 344, 782, 856, 975
Bse118I	RCCGGY	2 cut(s)	337, 584
Bse1I	ACTGG	2 cut(s)	308, 776
Bse3DI	GCAATG	1 cut(s)	891
Bse8I	GATNNNNATC	1 cut(s)	657
BseAI	TCCGGA	1 cut(s)	649
BseCI	ATCGAT	1 cut(s)	168
BseDI	CCNNGG	2 cut(s)	244, 666
BseGI	GGATG	3 cut(s)	349, 611, 708
BseJI	GATNNNNATC	1 cut(s)	657
BseLI	CCNNNNNNNGG	5 cut(s)	127, 344, 782, 856, 975
BseMI	GCAATG	1 cut(s)	891
BseMII	CTCAG	2 cut(s)	159, 950
BseNI	ACTGG	2 cut(s)	308, 776
BseYI	CCCAGC	1 cut(s)	553
BsgI	GTGCAG	1 cut(s)	56
Bsh1236I	CGCG	1 cut(s)	782
Bsh1285I	CGRYCG	1 cut(s)	338
BshFI	GGCC	6 cut(s)	457, 584, 588, 677, 910, 979
BshNI	GGYRCC	1 cut(s)	752
BshVI	ATCGAT	1 cut(s)	168
BsiEI	CGRYCG	1 cut(s)	338
BsiSI	CCGG	7 cut(s)	83, 338, 585, 650, 756, 856, 871
BslFI	GGGAC	1 cut(s)	821
BslI	CCNNNNNNNGG	5 cut(s)	127, 344, 782, 856, 975
BsmFI	GGGAC	1 cut(s)	821
BsmI	GAATGC	1 cut(s)	558
BsnI	GGCC	6 cut(s)	457, 584, 588, 677, 910, 979
Bsp13I	TCCGGA	1 cut(s)	649
Bsp143I	GATC	5 cut(s)	162, 169, 548, 652, 1054
Bsp19I	CCATGG	2 cut(s)	244, 666
BspACI	CCGC	5 cut(s)	330, 341, 521, 773, 782
BspANI	GGCC	6 cut(s)	457, 584, 588, 677, 910, 979
BspCNI	CTCAG	2 cut(s)	158, 949
BspDI	ATCGAT	1 cut(s)	168
BspEI	TCCGGA	1 cut(s)	649
BspFNI	CGCG	1 cut(s)	782
BspHI	TCATGA	2 cut(s)	268, 745
BspLI	GGNNCC	5 cut(s)	458, 654, 754, 771, 809
BspMAI	CTGCAG	1 cut(s)	58
BspOI	GCTAGC	1 cut(s)	521
BspPI	GGATC	4 cut(s)	556, 647, 660, 1049
BspT107I	GGYRCC	1 cut(s)	752
BsrBI	CCGCTC	1 cut(s)	332
BsrDI	GCAATG	1 cut(s)	891
BsrFI	RCCGGY	2 cut(s)	337, 584
BsrI	ACTGG	2 cut(s)	308, 776
BssAI	RCCGGY	2 cut(s)	337, 584
BssECI	CCNNGG	2 cut(s)	244, 666
BssMI	GATC	5 cut(s)	162, 169, 548, 652, 1054
BssNI	GRCGYC	1 cut(s)	753
BssSI	CACGAG	1 cut(s)	74
BssT1I	CCWWGG	2 cut(s)	244, 666
Bst2BI	CACGAG	1 cut(s)	74
Bst4CI	ACNGT	1 cut(s)	769
BstACI	GRCGYC	1 cut(s)	753
BstAPI	GCANNNNNTGC	1 cut(s)	617
BstC8I	GCNNGC	4 cut(s)	424, 519, 586, 734
BstDEI	CTNAG	2 cut(s)	145, 936
BstDSI	CCRYGG	2 cut(s)	244, 666
BstF5I	GGATG	3 cut(s)	349, 611, 708
BstFNI	CGCG	1 cut(s)	782
BstH2I	RGCGCY	1 cut(s)	756
BstHHI	GCGC	1 cut(s)	755
BstKTI	GATC	5 cut(s)	165, 172, 551, 655, 1057
BstMBI	GATC	5 cut(s)	162, 169, 548, 652, 1054
BstMCI	CGRYCG	1 cut(s)	338
BstMWI	GCNNNNNNNGC	3 cut(s)	313, 338, 617
BstNSI	RCATGY	3 cut(s)	209, 213, 648
BstSCI	CCNGG	2 cut(s)	755, 855
BstSFI	CTRYAG	1 cut(s)	54
BstUI	CGCG	1 cut(s)	782
BstV2I	GAAGAC	2 cut(s)	576, 1021
BstX2I	RGATCY	1 cut(s)	652
BstXI	CCANNNNNNTGG	2 cut(s)	673, 987
BstYI	RGATCY	1 cut(s)	652
Bsu15I	ATCGAT	1 cut(s)	168
BsuRI	GGCC	6 cut(s)	457, 584, 588, 677, 910, 979
BsuTUI	ATCGAT	1 cut(s)	168
BtgI	CCRYGG	2 cut(s)	244, 666
BtsCI	GGATG	3 cut(s)	349, 611, 708
BtsI	GCAGTG	1 cut(s)	68
BtsIMutI	CAGTG	1 cut(s)	68
Cac8I	GCNNGC	4 cut(s)	424, 519, 586, 734
CciI	TCATGA	2 cut(s)	268, 745
CfoI	GCGC	1 cut(s)	755
Cfr10I	RCCGGY	2 cut(s)	337, 584
Cfr13I	GGNCC	5 cut(s)	456, 582, 587, 808, 908
ClaI	ATCGAT	1 cut(s)	168
Csp6I	GTAC	3 cut(s)	118, 838, 997
CspCI	CAANNNNNGTGG	2 cut(s)	709, 744
CviQI	GTAC	3 cut(s)	118, 838, 997
DdeI	CTNAG	2 cut(s)	145, 936
DinI	GGCGCC	1 cut(s)	754
DpnI	GATC	5 cut(s)	164, 171, 550, 654, 1056
DpnII	GATC	5 cut(s)	162, 169, 548, 652, 1054
EaeI	YGGCCR	2 cut(s)	675, 977
EciI	GGCGGA	2 cut(s)	356, 762
Eco130I	CCWWGG	2 cut(s)	244, 666
Eco47I	GGWCC	1 cut(s)	808
EcoO109I	RGGNCCY	1 cut(s)	808
EcoT14I	CCWWGG	2 cut(s)	244, 666
EcoT22I	ATGCAT	3 cut(s)	97, 211, 613
EgeI	GGCGCC	1 cut(s)	754
EheI	GGCGCC	1 cut(s)	754
ErhI	CCWWGG	2 cut(s)	244, 666
FalI	AAGNNNNNCTT	2 cut(s)	1002, 1034
FaqI	GGGAC	1 cut(s)	821
FauI	CCCGC	2 cut(s)	514, 775
FauNDI	CATATG	1 cut(s)	91
Fnu4HI	GCNGC	1 cut(s)	330
FokI	GGATG	3 cut(s)	356, 598, 695
FseI	GGCCGGCC	1 cut(s)	588
Fsp4HI	GCNGC	1 cut(s)	330
FspBI	CTAG	2 cut(s)	518, 918
GlaI	GCGC	1 cut(s)	754
GluI	GCNGC	1 cut(s)	330
GsaI	CCCAGC	1 cut(s)	557
HaeII	RGCGCY	1 cut(s)	756
HaeIII	GGCC	6 cut(s)	457, 584, 588, 677, 910, 979
HapII	CCGG	7 cut(s)	83, 338, 585, 650, 756, 856, 871
HhaI	GCGC	1 cut(s)	755
Hin1I	GRCGYC	1 cut(s)	753
Hin6I	GCGC	1 cut(s)	753
HinP1I	GCGC	1 cut(s)	753
HincII	GTYRAC	2 cut(s)	799, 878
HindII	GTYRAC	2 cut(s)	799, 878
HindIII	AAGCTT	2 cut(s)	260, 1046
HinfI	GANTC	9 cut(s)	12, 86, 100, 131, 249, 278, 363, 689, 874
HpaII	CCGG	7 cut(s)	83, 338, 585, 650, 756, 856, 871
HphI	GGTGA	6 cut(s)	170, 420, 608, 845, 943, 953
Hpy166II	GTNNAC	4 cut(s)	799, 838, 868, 878
Hpy188I	TCNGA	5 cut(s)	19, 148, 162, 406, 564
Hpy188III	TCNNGA	4 cut(s)	128, 269, 650, 746
Hpy8I	GTNNAC	4 cut(s)	799, 838, 868, 878
Hpy99I	CGWCG	1 cut(s)	116
HpyAV	CCTTC	3 cut(s)	559, 860, 985
HpyCH4III	ACNGT	1 cut(s)	769
HpyCH4IV	ACGT	2 cut(s)	573, 883
HpyCH4V	TGCA	8 cut(s)	56, 73, 95, 209, 611, 644, 736, 1004
HpyF10VI	GCNNNNNNNGC	3 cut(s)	313, 338, 617
HpyF3I	CTNAG	2 cut(s)	145, 936
HpySE526I	ACGT	2 cut(s)	573, 883
Hsp92I	GRCGYC	1 cut(s)	753
HspAI	GCGC	1 cut(s)	753
KasI	GGCGCC	1 cut(s)	752
Kpn2I	TCCGGA	1 cut(s)	649
KroI	GCCGGC	1 cut(s)	584
KroNI	GCCGGC	1 cut(s)	586
Kzo9I	GATC	5 cut(s)	162, 169, 548, 652, 1054
LmnI	GCTCC	1 cut(s)	785
LweI	GCATC	3 cut(s)	10, 598, 620
MaeI	CTAG	2 cut(s)	518, 918
MaeII	ACGT	2 cut(s)	573, 883
MaeIII	GTNAC	5 cut(s)	78, 235, 596, 949, 959
MalI	GATC	5 cut(s)	164, 171, 550, 654, 1056
MbiI	CCGCTC	1 cut(s)	332
MboI	GATC	5 cut(s)	162, 169, 548, 652, 1054
MboII	GAAGA	7 cut(s)	257, 369, 503, 581, 617, 734, 1026
MfeI	CAATTG	1 cut(s)	861
MflI	RGATCY	1 cut(s)	652
MluCI	AATT	7 cut(s)	473, 576, 626, 631, 861, 1041, 1075
Mly113I	GGCGCC	1 cut(s)	753
MlyI	GAGTC	4 cut(s)	80, 94, 125, 883
MmeI	TCCRAC	2 cut(s)	384, 852
MnlI	CCTC	6 cut(s)	400, 707, 742, 945, 975, 1068
Mph1103I	ATGCAT	3 cut(s)	97, 211, 613
MroI	TCCGGA	1 cut(s)	649
MroNI	GCCGGC	1 cut(s)	584
MroXI	GAANNNNTTC	1 cut(s)	441
MseI	TTAA	4 cut(s)	192, 539, 630, 1044
MslI	CAYNNNNRTG	2 cut(s)	94, 183
MspI	CCGG	7 cut(s)	83, 338, 585, 650, 756, 856, 871
MspR9I	CCNGG	2 cut(s)	757, 857
MunI	CAATTG	1 cut(s)	861
Mva1269I	GAATGC	1 cut(s)	558
MvnI	CGCG	1 cut(s)	782
MwoI	GCNNNNNNNGC	3 cut(s)	313, 338, 617
NaeI	GCCGGC	1 cut(s)	586
NarI	GGCGCC	1 cut(s)	753
NciI	CCSGG	2 cut(s)	757, 857
NcoI	CCATGG	2 cut(s)	244, 666
NdeI	CATATG	1 cut(s)	91
NdeII	GATC	5 cut(s)	162, 169, 548, 652, 1054
NgoMIV	GCCGGC	1 cut(s)	584
NheI	GCTAGC	1 cut(s)	517
NlaIV	GGNNCC	5 cut(s)	458, 654, 754, 771, 809
NmuCI	GTSAC	4 cut(s)	235, 596, 949, 959
NsiI	ATGCAT	3 cut(s)	97, 211, 613
NspI	RCATGY	3 cut(s)	209, 213, 648
PagI	TCATGA	2 cut(s)	268, 745
PcsI	WCGNNNNNNNCGW	1 cut(s)	938
PctI	GAATGC	1 cut(s)	558
PdiI	GCCGGC	1 cut(s)	586
PdmI	GAANNNNTTC	1 cut(s)	441
PfeI	GAWTC	5 cut(s)	12, 249, 278, 363, 689
PkrI	GCNGC	1 cut(s)	331
PleI	GAGTC	4 cut(s)	80, 94, 125, 882
PluTI	GGCGCC	1 cut(s)	756
PpsI	GAGTC	4 cut(s)	80, 94, 125, 882
PpuMI	RGGWCCY	1 cut(s)	808
Psp5II	RGGWCCY	1 cut(s)	808
PspFI	CCCAGC	1 cut(s)	553
PspN4I	GGNNCC	5 cut(s)	458, 654, 754, 771, 809
PspPI	GGNCC	5 cut(s)	456, 582, 587, 808, 908
PspPPI	RGGWCCY	1 cut(s)	808
PstI	CTGCAG	1 cut(s)	58
PsuI	RGATCY	1 cut(s)	652
RigI	GGCCGGCC	1 cut(s)	588
RsaI	GTAC	3 cut(s)	119, 839, 998
RsaNI	GTAC	3 cut(s)	118, 838, 997
RseI	CAYNNNNRTG	2 cut(s)	94, 183
SaqAI	TTAA	4 cut(s)	192, 539, 630, 1044
SatI	GCNGC	1 cut(s)	330
Sau3AI	GATC	5 cut(s)	162, 169, 548, 652, 1054
Sau96I	GGNCC	5 cut(s)	456, 582, 587, 808, 908
SchI	GAGTC	4 cut(s)	80, 94, 125, 883
ScrFI	CCNGG	2 cut(s)	757, 857
SfaNI	GCATC	3 cut(s)	10, 598, 620
SfcI	CTRYAG	1 cut(s)	54
SfoI	GGCGCC	1 cut(s)	754
SinI	GGWCC	1 cut(s)	808
SmiMI	CAYNNNNRTG	2 cut(s)	94, 183
Sse9I	AATT	7 cut(s)	473, 576, 626, 631, 861, 1041, 1075
SsiI	CCGC	5 cut(s)	330, 341, 521, 773, 782
SspDI	GGCGCC	1 cut(s)	752
SspI	AATATT	1 cut(s)	543
SspMI	CTAG	2 cut(s)	518, 918
StyD4I	CCNGG	2 cut(s)	755, 855
StyI	CCWWGG	2 cut(s)	244, 666
TaaI	ACNGT	1 cut(s)	769
TaiI	ACGT	2 cut(s)	576, 886
TaqI	TCGA	7 cut(s)	111, 168, 289, 334, 435, 445, 941
TasI	AATT	7 cut(s)	473, 576, 626, 631, 861, 1041, 1075
TauI	GCSGC	1 cut(s)	332
TfiI	GAWTC	5 cut(s)	12, 249, 278, 363, 689
Tru1I	TTAA	4 cut(s)	192, 539, 630, 1044
Tru9I	TTAA	4 cut(s)	192, 539, 630, 1044
TscAI	CASTG	1 cut(s)	75
TseFI	GTSAC	4 cut(s)	235, 596, 949, 959
Tsp45I	GTSAC	4 cut(s)	235, 596, 949, 959
TspDTI	ATGAA	4 cut(s)	257, 524, 734, 1026
TspGWI	ACGGA	1 cut(s)	701
TspRI	CASTG	1 cut(s)	75
VpaK11BI	GGWCC	1 cut(s)	808
XapI	RAATTY	1 cut(s)	473
XceI	RCATGY	3 cut(s)	209, 213, 648
XcmI	CCANNNNNNNNNTGG	1 cut(s)	664
XmnI	GAANNNNTTC	1 cut(s)	441
XspI	CTAG	2 cut(s)	518, 918
Zsp2I	ATGCAT	3 cut(s)	97, 211, 613

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.