MD07G1194100.v1.1

THO complex subunit 4A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004491
Physical Location & Seq
Forward (+)
27362245 .. 27364805
2561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1194100.v1.1.491

Sequence Viewer

Length: 756 bp
ATGCCCCCGCCGCTGGACATGTCGCTCGACGATCTCATATGGAGGCGCAAGGCGAAGAAGTCGGAAGCCGGAGGGCATCGCCGTGATTTCAGAGTCCCCCCAGGCGGCCGAGGCTCCGCGCCGGGACCCACTCGTCAGCTCGTGAAACGCAACCCCGTTAGAACGGCGCCCTACTTTCCTAAACCGAAAATGCAAGTTCCAAGATCCAGATTTGAGCAAGAAATGGATGTGACCATGGGGACAAACTCAGAGAAGGGAACCAAATTATACATATCTAACTTGGATTATGAGGTTTCGAATAGCGATATTGAGTTGCTCTTCTCTGATATTGGTCATGTGATACACCATTCAATCCATTATGATAGGAGTGGAAGATCAAAGGGAACGGCGGAAGTTAGATATCTACACCATTCAGATGCTCTAACAGCAATCAAGAAATATAACGATGTTCAGCTTGATGGAAAGCCAATGAAAATTGAGCTTGTGGGAGTGAGTCCTGTTGCTCCTGTTCCTGTGCCTCCAATTACAAGTAGATTGATGGGAAAACCAAACGTTGTCTTCCAAAGTGGACGAGGTCAAGCTGGTGGTAGGGGATTCTTTCATGGTCGTGGTACCGGTGGCTTTGGACTTGGAAGGGGCCATGGACGGGTGAAGCAGCCTCGTGAGCAGGTGAAGCCGACTCGTGAGAAAGTGACTTTTGAAGATCTGGATGCAGACTTGGAGAACTACCGCCAAAAACGCTTGCTGGTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

252

Amino Acids

28.05

Weight (kDa)

10.08

Isoelectric Point (pI)

48.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 89 - 157 8.8e-14 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 658
AasI GACNNNNNNGTC 1 cut(s) 132
Acc36I ACCTGC 1 cut(s) 658
Acc65I GGTACC 1 cut(s) 611
AccB1I GGYRCC 2 cut(s) 166, 611
AccII CGCG 1 cut(s) 119
AciI CCGC 6 cut(s) 8, 11, 105, 117, 389, 730
AclI AACGTT 1 cut(s) 552
AclWI GGATC 1 cut(s) 198
AcoI YGGCCR 1 cut(s) 106
AcyI GRCGYC 1 cut(s) 167
AfaI GTAC 1 cut(s) 613
AfiI CCNNNNNNNGG 3 cut(s) 13, 104, 646
AflIII ACRYGT 1 cut(s) 18
AgeI ACCGGT 1 cut(s) 614
AgsI TTSAA 2 cut(s) 351, 701
AjnI CCWGG 1 cut(s) 100
AluBI AGCT 4 cut(s) 139, 454, 481, 581
AluI AGCT 4 cut(s) 139, 454, 481, 581
AlwI GGATC 1 cut(s) 198
AoxI GGCC 2 cut(s) 106, 637
ApeKI GCWGC 1 cut(s) 655
AsiGI ACCGGT 1 cut(s) 614
Asp718I GGTACC 1 cut(s) 611
AspLEI GCGC 3 cut(s) 48, 121, 169
AspS9I GGNCC 2 cut(s) 125, 637
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 2 cut(s) 661, 682
AsuII TTCGAA 1 cut(s) 296
AvaII GGWCC 1 cut(s) 125
BanI GGYRCC 2 cut(s) 166, 611
BauI CACGAG 3 cut(s) 140, 660, 681
BbsI GAAGAC 1 cut(s) 550
BbvI GCAGC 1 cut(s) 667
BccI CCATC 2 cut(s) 452, 532
BceAI ACGGC 3 cut(s) 66, 180, 402
BciT130I CCWGG 1 cut(s) 102
BcnI CCSGG 1 cut(s) 123
BfoI RGCGCY 1 cut(s) 170
BfuAI ACCTGC 1 cut(s) 658
BglII AGATCT 1 cut(s) 703
BisI GCNGC 3 cut(s) 11, 106, 656
BlsI GCNGC 3 cut(s) 12, 107, 657
Bme1390I CCNGG 2 cut(s) 102, 123
Bme18I GGWCC 1 cut(s) 125
BmgT120I GGNCC 2 cut(s) 125, 637
BmiI GGNNCC 7 cut(s) 115, 126, 127, 168, 259, 613, 638
BmrFI CCNGG 2 cut(s) 102, 123
BmsI GCATC 3 cut(s) 85, 406, 700
BpiI GAAGAC 1 cut(s) 550
Bpu14I TTCGAA 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 123
BsaHI GRCGYC 1 cut(s) 167
BsaJI CCNNGG 4 cut(s) 100, 109, 234, 640
BsaWI WCCGGW 1 cut(s) 614
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 104, 646
Bse118I RCCGGY 1 cut(s) 614
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 4 cut(s) 100, 109, 234, 640
BseGI GGATG 2 cut(s) 232, 715
BseLI CCNNNNNNNGG 3 cut(s) 13, 104, 646
BseMII CTCAG 1 cut(s) 261
BseX3I CGGCCG 1 cut(s) 106
BseXI GCAGC 1 cut(s) 667
Bsh1236I CGCG 1 cut(s) 119
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 2 cut(s) 108, 639
BshNI GGYRCC 2 cut(s) 166, 611
BshTI ACCGGT 1 cut(s) 614
BsiEI CGRYCG 1 cut(s) 109
BsiSI CCGG 3 cut(s) 69, 122, 615
BslFI GGGAC 3 cut(s) 80, 138, 253
BslI CCNNNNNNNGG 3 cut(s) 13, 104, 646
BsmFI GGGAC 3 cut(s) 80, 138, 253
BsnI GGCC 2 cut(s) 108, 639
Bsp119I TTCGAA 1 cut(s) 296
Bsp143I GATC 4 cut(s) 31, 203, 374, 703
Bsp19I CCATGG 2 cut(s) 234, 640
BspACI CCGC 6 cut(s) 8, 11, 105, 117, 389, 730
BspANI GGCC 2 cut(s) 108, 639
BspCNI CTCAG 1 cut(s) 260
BspFNI CGCG 1 cut(s) 119
BspLI GGNNCC 7 cut(s) 115, 126, 127, 168, 259, 613, 638
BspMI ACCTGC 1 cut(s) 658
BspPI GGATC 1 cut(s) 198
BspQI GCTCTTC 1 cut(s) 323
BspT104I TTCGAA 1 cut(s) 296
BspT107I GGYRCC 2 cut(s) 166, 611
BsrFI RCCGGY 1 cut(s) 614
BssAI RCCGGY 1 cut(s) 614
BssECI CCNNGG 4 cut(s) 100, 109, 234, 640
BssMI GATC 4 cut(s) 31, 203, 374, 703
BssNI GRCGYC 1 cut(s) 167
BssSI CACGAG 3 cut(s) 140, 660, 681
BssT1I CCWWGG 2 cut(s) 234, 640
Bst2BI CACGAG 3 cut(s) 140, 660, 681
Bst2UI CCWGG 1 cut(s) 102
Bst6I CTCTTC 1 cut(s) 323
BstACI GRCGYC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 743
BstDEI CTNAG 1 cut(s) 247
BstDSI CCRYGG 2 cut(s) 234, 640
BstF5I GGATG 2 cut(s) 232, 715
BstFNI CGCG 1 cut(s) 119
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 3 cut(s) 48, 121, 169
BstKTI GATC 4 cut(s) 34, 206, 377, 706
BstMBI GATC 4 cut(s) 31, 203, 374, 703
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 6 cut(s) 10, 111, 425, 664, 673, 738
BstNI CCWGG 1 cut(s) 102
BstNSI RCATGY 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 100, 121
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 1 cut(s) 667
BstV2I GAAGAC 1 cut(s) 550
BstX2I RGATCY 2 cut(s) 203, 703
BstYI RGATCY 2 cut(s) 203, 703
BstZI CGGCCG 1 cut(s) 106
BsuRI GGCC 2 cut(s) 108, 639
BtgI CCRYGG 2 cut(s) 234, 640
BtgZI GCGATG 1 cut(s) 62
BtsCI GGATG 2 cut(s) 232, 715
BveI ACCTGC 1 cut(s) 658
Cac8I GCNNGC 1 cut(s) 743
CfoI GCGC 3 cut(s) 48, 121, 169
Cfr10I RCCGGY 1 cut(s) 614
Cfr13I GGNCC 2 cut(s) 125, 637
Csp6I GTAC 1 cut(s) 612
CspAI ACCGGT 1 cut(s) 614
CviAII CATG 5 cut(s) 19, 235, 335, 602, 641
CviQI GTAC 1 cut(s) 612
DdeI CTNAG 1 cut(s) 247
DinI GGCGCC 1 cut(s) 168
DpnI GATC 4 cut(s) 33, 205, 376, 705
DpnII GATC 4 cut(s) 31, 203, 374, 703
DrdI GACNNNNNNGTC 1 cut(s) 132
DseDI GACNNNNNNGTC 1 cut(s) 132
EaeI YGGCCR 1 cut(s) 106
EagI CGGCCG 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 323
EciI GGCGGA 1 cut(s) 404
EclXI CGGCCG 1 cut(s) 106
Eco130I CCWWGG 2 cut(s) 234, 640
Eco32I GATATC 1 cut(s) 401
Eco47I GGWCC 1 cut(s) 125
Eco52I CGGCCG 1 cut(s) 106
EcoO109I RGGNCCY 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 100
EcoRV GATATC 1 cut(s) 401
EcoT14I CCWWGG 2 cut(s) 234, 640
EgeI GGCGCC 1 cut(s) 168
EheI GGCGCC 1 cut(s) 168
ErhI CCWWGG 2 cut(s) 234, 640
FaeI CATG 5 cut(s) 22, 238, 338, 605, 644
FaqI GGGAC 3 cut(s) 80, 138, 253
FatI CATG 5 cut(s) 18, 234, 334, 601, 640
FauI CCCGC 1 cut(s) 15
FauNDI CATATG 1 cut(s) 38
Fnu4HI GCNGC 3 cut(s) 11, 106, 656
FokI GGATG 2 cut(s) 239, 722
Fsp4HI GCNGC 3 cut(s) 11, 106, 656
GlaI GCGC 3 cut(s) 47, 120, 168
GluI GCNGC 3 cut(s) 11, 106, 656
HaeII RGCGCY 1 cut(s) 170
HaeIII GGCC 2 cut(s) 108, 639
HapII CCGG 3 cut(s) 69, 122, 615
HhaI GCGC 3 cut(s) 48, 121, 169
Hin1I GRCGYC 1 cut(s) 167
Hin1II CATG 5 cut(s) 22, 238, 338, 605, 644
Hin6I GCGC 3 cut(s) 46, 119, 167
HinP1I GCGC 3 cut(s) 46, 119, 167
HinfI GANTC 4 cut(s) 93, 493, 594, 679
HpaII CCGG 3 cut(s) 69, 122, 615
HphI GGTGA 2 cut(s) 661, 682
Hpy166II GTNNAC 1 cut(s) 569
Hpy188I TCNGA 5 cut(s) 64, 92, 250, 325, 415
Hpy188III TCNNGA 6 cut(s) 142, 207, 433, 662, 683, 707
Hpy8I GTNNAC 1 cut(s) 569
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 247, 627
HpyCH4IV ACGT 1 cut(s) 552
HpyCH4V TGCA 2 cut(s) 193, 713
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 111, 425, 664, 673, 738
HpyF3I CTNAG 1 cut(s) 247
HpySE526I ACGT 1 cut(s) 552
Hsp92I GRCGYC 1 cut(s) 167
Hsp92II CATG 5 cut(s) 22, 238, 338, 605, 644
HspAI GCGC 3 cut(s) 46, 119, 167
KasI GGCGCC 1 cut(s) 166
KflI GGGWCCC 1 cut(s) 125
KpnI GGTACC 1 cut(s) 615
Kzo9I GATC 4 cut(s) 31, 203, 374, 703
LguI GCTCTTC 1 cut(s) 323
LmnI GCTCC 2 cut(s) 119, 508
Lsp1109I GCAGC 1 cut(s) 667
LweI GCATC 3 cut(s) 85, 406, 700
MaeII ACGT 1 cut(s) 552
MaeIII GTNAC 2 cut(s) 229, 691
MalI GATC 4 cut(s) 33, 205, 376, 705
MboI GATC 4 cut(s) 31, 203, 374, 703
MboII GAAGA 5 cut(s) 67, 310, 384, 550, 713
MflI RGATCY 2 cut(s) 203, 703
MluCI AATT 4 cut(s) 263, 474, 522, 751
Mly113I GGCGCC 1 cut(s) 167
MlyI GAGTC 3 cut(s) 102, 502, 673
MmeI TCCRAC 1 cut(s) 42
MnlI CCTC 7 cut(s) 36, 65, 104, 283, 528, 566, 669
MslI CAYNNNNRTG 3 cut(s) 81, 414, 606
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 3 cut(s) 69, 122, 615
MspR9I CCNGG 2 cut(s) 102, 123
MvaI CCWGG 1 cut(s) 102
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 6 cut(s) 10, 111, 425, 664, 673, 738
NarI GGCGCC 1 cut(s) 167
NciI CCSGG 1 cut(s) 123
NcoI CCATGG 2 cut(s) 234, 640
NdeI CATATG 1 cut(s) 38
NdeII GATC 4 cut(s) 31, 203, 374, 703
NlaIII CATG 5 cut(s) 22, 238, 338, 605, 644
NlaIV GGNNCC 7 cut(s) 115, 126, 127, 168, 259, 613, 638
NmeAIII GCCGAG 1 cut(s) 134
NmuCI GTSAC 2 cut(s) 229, 691
NspI RCATGY 1 cut(s) 22
NspV TTCGAA 1 cut(s) 296
PaqCI CACCTGC 1 cut(s) 658
PciI ACATGT 1 cut(s) 18
PciSI GCTCTTC 1 cut(s) 323
PfeI GAWTC 1 cut(s) 594
PflFI GACNNNGTC 1 cut(s) 573
PinAI ACCGGT 1 cut(s) 614
PkrI GCNGC 3 cut(s) 12, 107, 657
PleI GAGTC 3 cut(s) 101, 501, 673
PluTI GGCGCC 1 cut(s) 170
PpsI GAGTC 3 cut(s) 101, 501, 673
PpuMI RGGWCCY 1 cut(s) 125
PscI ACATGT 1 cut(s) 18
Psp1406I AACGTT 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 125
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 7 cut(s) 115, 126, 127, 168, 259, 613, 638
PspPI GGNCC 2 cut(s) 125, 637
PspPPI RGGWCCY 1 cut(s) 125
PsuI RGATCY 2 cut(s) 203, 703
PsyI GACNNNGTC 1 cut(s) 573
RsaI GTAC 1 cut(s) 613
RsaNI GTAC 1 cut(s) 612
RseI CAYNNNNRTG 3 cut(s) 81, 414, 606
SapI GCTCTTC 1 cut(s) 323
SatI GCNGC 3 cut(s) 11, 106, 656
Sau3AI GATC 4 cut(s) 31, 203, 374, 703
Sau96I GGNCC 2 cut(s) 125, 637
SchI GAGTC 3 cut(s) 102, 502, 673
ScrFI CCNGG 2 cut(s) 102, 123
SetI ASST 8 cut(s) 141, 294, 456, 483, 555, 577, 583, 672
SfaNI GCATC 3 cut(s) 85, 406, 700
SfoI GGCGCC 1 cut(s) 168
SfuI TTCGAA 1 cut(s) 296
SinI GGWCC 1 cut(s) 125
SmiMI CAYNNNNRTG 3 cut(s) 81, 414, 606
Sse9I AATT 4 cut(s) 263, 474, 522, 751
SsiI CCGC 6 cut(s) 8, 11, 105, 117, 389, 730
SspDI GGCGCC 1 cut(s) 166
StyD4I CCNGG 2 cut(s) 100, 121
StyI CCWWGG 2 cut(s) 234, 640
TaiI ACGT 1 cut(s) 555
TaqI TCGA 2 cut(s) 27, 296
TasI AATT 4 cut(s) 263, 474, 522, 751
TauI GCSGC 2 cut(s) 13, 108
TfiI GAWTC 1 cut(s) 594
TseFI GTSAC 2 cut(s) 229, 691
TseI GCWGC 1 cut(s) 655
Tsp45I GTSAC 2 cut(s) 229, 691
TspDTI ATGAA 2 cut(s) 485, 590
Tth111I GACNNNGTC 1 cut(s) 573
VpaK11BI GGWCC 1 cut(s) 125
XceI RCATGY 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.