Rw1G029840

THO complex subunit 4A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004491
Physical Location & Seq
Forward (+)
57540157 .. 57542855
2699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G029840.1

Sequence Viewer

Length: 873 bp
ATGCCCTTCCCTCAGGACCGCCTGGACCTCCCTCTCGACGCCCTCATCGCCGAATCCAAGCAACAACGCCGTCACAACAACCAGGCTCACCACCCACGAGGGCATACCGGTCATTTCGGCGGCAGAGGCCACGGAGGCTCCACCTCAGGCCCCGCTCGTCGGCCGATGAACCGCAACGCCTACAGAACGGCGCCGTATCCCACTCTGCCGCAAATGCAAGGACAGCCTGGACCCAGAAGGATTGAGTATCCATTGGATTTGAGCCAAGAGATGAACAGAGAGGAAAGCACCAAGCTTTATATATCTAACTTGGACTATGATGTTACCAATAGAGATATAGAGTTGCTCTTCTCTGAGGTTGGTGAGTTGGAAAGGCACTCAATCCATTATGATAAAAGTGGAAGATCTAAGGGAACAGCTGAAGTTGTCTATAAGCACCATTCAGATGCTCTAGCAGCAATCAAGAGATATAACAATCAGCATCTTGATGGAAAGAAAGTGGAAATTAAGCTTGTGGGACTCAATGTTGTTTCTCCTGTTCCTCTGCTTCTGCCTCCAAATAAAAATTTCATGCAAGAACATCCAACTCCCGTCTTCCAGAGGCTAGGAAGAGCTGGTTCAAGGGGATCGTTTCATGGTCATGGTGGTGTTGGACTTGAAAGAGGCTGTGGACAGGTGAGAAATGGTGGTCCGAAGGTTACTACAGAATCTCAGCAATGGAAGAATGTGACTGCAGAAAATCAGCAAAGGAAGAATGTGATTACAGAATCTGTTAAAAGGAAGAAAACATTTTATGGAGATACAACTGTGACTTTTGATGATCTGGATGCTGATTTGGAGAAATACCGCCTAAAATGTAGGCCGGTAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

290

Amino Acids

32.74

Weight (kDa)

9.62

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 99 - 167 6.6e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 190
AccBSI CCGCTC 1 cut(s) 155
AciI CCGC 6 cut(s) 19, 120, 153, 172, 209, 847
AclWI GGATC 1 cut(s) 634
AcoI YGGCCR 1 cut(s) 161
AcsI RAATTY 1 cut(s) 565
AcuI CTGAAG 1 cut(s) 441
AcyI GRCGYC 2 cut(s) 39, 191
AfiI CCNNNNNNNGG 1 cut(s) 159
AgeI ACCGGT 1 cut(s) 107
AgsI TTSAA 2 cut(s) 621, 659
AjnI CCWGG 3 cut(s) 21, 81, 226
AluBI AGCT 4 cut(s) 295, 419, 511, 614
AluI AGCT 4 cut(s) 295, 419, 511, 614
AlwI GGATC 1 cut(s) 634
AlwNI CAGNNNCTG 1 cut(s) 770
AoxI GGCC 4 cut(s) 127, 148, 161, 860
ApeKI GCWGC 1 cut(s) 455
ApoI RAATTY 1 cut(s) 565
AsiGI ACCGGT 1 cut(s) 107
AspLEI GCGC 1 cut(s) 193
AspS9I GGNCC 5 cut(s) 16, 25, 149, 230, 689
AsuHPI GGTGA 3 cut(s) 80, 374, 688
AvaII GGWCC 4 cut(s) 16, 25, 230, 689
AxyI CCTNAGG 2 cut(s) 12, 145
BaeI ACNNNNGTAYC 2 cut(s) 792, 825
BanI GGYRCC 1 cut(s) 190
BauI CACGAG 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 586
BbvI GCAGC 1 cut(s) 467
BccI CCATC 1 cut(s) 482
BceAI ACGGC 3 cut(s) 54, 178, 204
BciT130I CCWGG 3 cut(s) 23, 83, 228
BciVI GTATCC 2 cut(s) 207, 258
BfaI CTAG 2 cut(s) 452, 605
BfmI CTRYAG 3 cut(s) 181, 702, 732
BfoI RGCGCY 1 cut(s) 194
BfuI GTATCC 2 cut(s) 207, 258
BglI GCCNNNNNGGC 1 cut(s) 135
BglII AGATCT 1 cut(s) 404
BisI GCNGC 3 cut(s) 121, 209, 456
BlsI GCNGC 3 cut(s) 122, 210, 457
Bme1390I CCNGG 3 cut(s) 23, 83, 228
Bme18I GGWCC 4 cut(s) 16, 25, 230, 689
BmgT120I GGNCC 5 cut(s) 16, 25, 149, 230, 689
BmiI GGNNCC 4 cut(s) 139, 151, 192, 232
BmrFI CCNGG 3 cut(s) 23, 83, 228
BmsI GCATC 3 cut(s) 436, 490, 817
BpiI GAAGAC 1 cut(s) 586
BsaHI GRCGYC 2 cut(s) 39, 191
BsaJI CCNNGG 1 cut(s) 130
BsaWI WCCGGW 1 cut(s) 107
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse118I RCCGGY 2 cut(s) 107, 862
Bse21I CCTNAGG 2 cut(s) 12, 145
Bse3DI GCAATG 1 cut(s) 722
BseBI CCWGG 3 cut(s) 23, 83, 228
BseDI CCNNGG 1 cut(s) 130
BseGI GGATG 2 cut(s) 580, 832
BseLI CCNNNNNNNGG 1 cut(s) 159
BseMI GCAATG 1 cut(s) 722
BseMII CTCAG 4 cut(s) 26, 159, 345, 725
BseX3I CGGCCG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 467
Bsh1285I CGRYCG 1 cut(s) 164
BshFI GGCC 4 cut(s) 129, 150, 163, 862
BshNI GGYRCC 1 cut(s) 190
BshTI ACCGGT 1 cut(s) 107
BsiEI CGRYCG 1 cut(s) 164
BsiSI CCGG 2 cut(s) 108, 863
BslFI GGGAC 1 cut(s) 531
BslI CCNNNNNNNGG 1 cut(s) 159
BsmFI GGGAC 1 cut(s) 531
BsnI GGCC 4 cut(s) 129, 150, 163, 862
Bsp143I GATC 3 cut(s) 404, 626, 820
BspACI CCGC 6 cut(s) 19, 120, 153, 172, 209, 847
BspANI GGCC 4 cut(s) 129, 150, 163, 862
BspCNI CTCAG 4 cut(s) 25, 158, 346, 724
BspLI GGNNCC 4 cut(s) 139, 151, 192, 232
BspMAI CTGCAG 1 cut(s) 736
BspPI GGATC 1 cut(s) 634
BspQI GCTCTTC 2 cut(s) 353, 604
BspT107I GGYRCC 1 cut(s) 190
BsrBI CCGCTC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 722
BsrFI RCCGGY 2 cut(s) 107, 862
BssAI RCCGGY 2 cut(s) 107, 862
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 3 cut(s) 404, 626, 820
BssNI GRCGYC 2 cut(s) 39, 191
BssSI CACGAG 1 cut(s) 96
Bst2BI CACGAG 1 cut(s) 96
Bst2UI CCWGG 3 cut(s) 23, 83, 228
Bst4CI ACNGT 1 cut(s) 808
Bst6I CTCTTC 2 cut(s) 353, 604
BstACI GRCGYC 2 cut(s) 39, 191
BstDEI CTNAG 5 cut(s) 12, 145, 354, 408, 711
BstDSI CCRYGG 1 cut(s) 130
BstF5I GGATG 2 cut(s) 580, 832
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 3 cut(s) 407, 629, 823
BstMBI GATC 3 cut(s) 404, 626, 820
BstMCI CGRYCG 1 cut(s) 164
BstMWI GCNNNNNNNGC 6 cut(s) 47, 126, 135, 214, 223, 455
BstNI CCWGG 3 cut(s) 23, 83, 228
BstSCI CCNGG 3 cut(s) 21, 81, 226
BstSFI CTRYAG 3 cut(s) 181, 702, 732
BstV1I GCAGC 1 cut(s) 467
BstV2I GAAGAC 1 cut(s) 586
BstX2I RGATCY 1 cut(s) 404
BstYI RGATCY 1 cut(s) 404
BstZI CGGCCG 1 cut(s) 161
Bsu36I CCTNAGG 2 cut(s) 12, 145
BsuI GTATCC 2 cut(s) 207, 258
BsuRI GGCC 4 cut(s) 129, 150, 163, 862
BtgI CCRYGG 1 cut(s) 130
BtgZI GCGATG 1 cut(s) 31
BtsCI GGATG 2 cut(s) 580, 832
CaiI CAGNNNCTG 1 cut(s) 770
CfoI GCGC 1 cut(s) 193
Cfr10I RCCGGY 2 cut(s) 107, 862
Cfr13I GGNCC 5 cut(s) 16, 25, 149, 230, 689
CseI GACGC 1 cut(s) 47
CspAI ACCGGT 1 cut(s) 107
CviAII CATG 3 cut(s) 571, 635, 641
DdeI CTNAG 5 cut(s) 12, 145, 354, 408, 711
DinI GGCGCC 1 cut(s) 192
DpnI GATC 3 cut(s) 406, 628, 822
DpnII GATC 3 cut(s) 404, 626, 820
EaeI YGGCCR 1 cut(s) 161
EagI CGGCCG 1 cut(s) 161
Eam1104I CTCTTC 2 cut(s) 353, 604
EarI CTCTTC 2 cut(s) 353, 604
EclXI CGGCCG 1 cut(s) 161
Eco47I GGWCC 4 cut(s) 16, 25, 230, 689
Eco52I CGGCCG 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 441
Eco81I CCTNAGG 2 cut(s) 12, 145
EcoO109I RGGNCCY 1 cut(s) 149
EcoRII CCWGG 3 cut(s) 21, 81, 226
EgeI GGCGCC 1 cut(s) 192
EheI GGCGCC 1 cut(s) 192
FaeI CATG 3 cut(s) 574, 638, 644
FaqI GGGAC 1 cut(s) 531
FatI CATG 3 cut(s) 570, 634, 640
FauI CCCGC 1 cut(s) 160
Fnu4HI GCNGC 3 cut(s) 121, 209, 456
FokI GGATG 2 cut(s) 567, 839
Fsp4HI GCNGC 3 cut(s) 121, 209, 456
FspBI CTAG 2 cut(s) 452, 605
GlaI GCGC 1 cut(s) 192
GluI GCNGC 3 cut(s) 121, 209, 456
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 4 cut(s) 129, 150, 163, 862
HapII CCGG 2 cut(s) 108, 863
HgaI GACGC 1 cut(s) 47
HhaI GCGC 1 cut(s) 193
Hin1I GRCGYC 2 cut(s) 39, 191
Hin1II CATG 3 cut(s) 574, 638, 644
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HindIII AAGCTT 2 cut(s) 293, 509
HinfI GANTC 4 cut(s) 53, 519, 707, 767
HpaII CCGG 2 cut(s) 108, 863
HphI GGTGA 3 cut(s) 80, 374, 688
Hpy166II GTNNAC 2 cut(s) 671, 868
Hpy188I TCNGA 3 cut(s) 355, 445, 693
Hpy188III TCNNGA 6 cut(s) 14, 35, 463, 485, 598, 824
Hpy8I GTNNAC 2 cut(s) 671, 868
Hpy99I CGWCG 2 cut(s) 41, 162
HpyAV CCTTC 3 cut(s) 16, 231, 688
HpyCH4III ACNGT 1 cut(s) 808
HpyCH4V TGCA 3 cut(s) 217, 574, 734
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 126, 135, 214, 223, 455
HpyF3I CTNAG 5 cut(s) 12, 145, 354, 408, 711
Hsp92I GRCGYC 2 cut(s) 39, 191
Hsp92II CATG 3 cut(s) 574, 638, 644
HspAI GCGC 1 cut(s) 191
KasI GGCGCC 1 cut(s) 190
Kzo9I GATC 3 cut(s) 404, 626, 820
LguI GCTCTTC 2 cut(s) 353, 604
LmnI GCTCC 1 cut(s) 143
Lsp1109I GCAGC 1 cut(s) 467
LweI GCATC 3 cut(s) 436, 490, 817
MaeI CTAG 2 cut(s) 452, 605
MaeIII GTNAC 5 cut(s) 71, 322, 697, 727, 808
MalI GATC 3 cut(s) 406, 628, 822
MbiI CCGCTC 1 cut(s) 155
MboI GATC 3 cut(s) 404, 626, 820
MboII GAAGA 7 cut(s) 340, 414, 586, 621, 733, 763, 793
MflI RGATCY 1 cut(s) 404
MluCI AATT 2 cut(s) 504, 565
Mly113I GGCGCC 1 cut(s) 191
MlyI GAGTC 1 cut(s) 513
MmeI TCCRAC 3 cut(s) 348, 608, 631
MseI TTAA 2 cut(s) 507, 774
MslI CAYNNNNRTG 4 cut(s) 444, 486, 639, 645
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 2 cut(s) 108, 863
MspR9I CCNGG 3 cut(s) 23, 83, 228
MvaI CCWGG 3 cut(s) 23, 83, 228
MwoI GCNNNNNNNGC 6 cut(s) 47, 126, 135, 214, 223, 455
NarI GGCGCC 1 cut(s) 191
NdeII GATC 3 cut(s) 404, 626, 820
NlaIII CATG 3 cut(s) 574, 638, 644
NlaIV GGNNCC 4 cut(s) 139, 151, 192, 232
NmuCI GTSAC 3 cut(s) 71, 727, 808
PciSI GCTCTTC 2 cut(s) 353, 604
PfeI GAWTC 3 cut(s) 53, 707, 767
PinAI ACCGGT 1 cut(s) 107
PkrI GCNGC 3 cut(s) 122, 210, 457
PleI GAGTC 1 cut(s) 513
PluTI GGCGCC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 513
Psp6I CCWGG 3 cut(s) 21, 81, 226
PspGI CCWGG 3 cut(s) 21, 81, 226
PspN4I GGNNCC 4 cut(s) 139, 151, 192, 232
PspPI GGNCC 5 cut(s) 16, 25, 149, 230, 689
PstI CTGCAG 1 cut(s) 736
PstNI CAGNNNCTG 1 cut(s) 770
PsuI RGATCY 1 cut(s) 404
PvuII CAGCTG 1 cut(s) 419
RseI CAYNNNNRTG 4 cut(s) 444, 486, 639, 645
SapI GCTCTTC 2 cut(s) 353, 604
SaqAI TTAA 2 cut(s) 507, 774
SatI GCNGC 3 cut(s) 121, 209, 456
Sau3AI GATC 3 cut(s) 404, 626, 820
Sau96I GGNCC 5 cut(s) 16, 25, 149, 230, 689
SchI GAGTC 1 cut(s) 513
ScrFI CCNGG 3 cut(s) 23, 83, 228
SetI ASST 9 cut(s) 30, 146, 297, 360, 421, 513, 616, 678, 699
SfaNI GCATC 3 cut(s) 436, 490, 817
SfcI CTRYAG 3 cut(s) 181, 702, 732
SfoI GGCGCC 1 cut(s) 192
SinI GGWCC 4 cut(s) 16, 25, 230, 689
SmiMI CAYNNNNRTG 4 cut(s) 444, 486, 639, 645
Sse9I AATT 2 cut(s) 504, 565
SsiI CCGC 6 cut(s) 19, 120, 153, 172, 209, 847
SspDI GGCGCC 1 cut(s) 190
SspMI CTAG 2 cut(s) 452, 605
StyD4I CCNGG 3 cut(s) 21, 81, 226
TaaI ACNGT 1 cut(s) 808
TaqI TCGA 1 cut(s) 36
TasI AATT 2 cut(s) 504, 565
TauI GCSGC 2 cut(s) 123, 211
TfiI GAWTC 3 cut(s) 53, 707, 767
Tru1I TTAA 2 cut(s) 507, 774
Tru9I TTAA 2 cut(s) 507, 774
TseFI GTSAC 3 cut(s) 71, 727, 808
TseI GCWGC 1 cut(s) 455
Tsp45I GTSAC 3 cut(s) 71, 727, 808
TspDTI ATGAA 4 cut(s) 182, 287, 559, 623
TspGWI ACGGA 1 cut(s) 147
VpaK11BI GGWCC 4 cut(s) 16, 25, 230, 689
XapI RAATTY 1 cut(s) 565
XspI CTAG 2 cut(s) 452, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.