Rorug02G0266100

THO complex subunit 4A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004491
Physical Location & Seq
Forward (+)
28124935 .. 28125642
708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0266100.1

Sequence Viewer

Length: 708 bp
ATGTTGGACGAAGCATATTTCGCCGGTGCGAAAGAGGGAGCGATTTCCAAGGACGGTTGGATGTACACGGTGGGAAAACATCTGGGCCGCTTCCGTTGGGATGACTTCTCCACCTGGCAAAATTTTTGCTTGCCATTTAATCCGTCAGATGCAAAGGTGCTTGCTGTTACTGAGACCGAGGTTTACCTGTTTTGCCCTTCATTCGGGGGTGGCGGTAGTATAGTCCGCGGCGATCTGAAATCTAACTGTTGGAAGACCCTTAATCTGAATTCTCCGTGTTCCCATTGGTCCTCCGGCGTGGTGCTGGAAAACAATTATTTGGTTTCCTTTGGAGTTCTGGAGCGCAACAGCATGCAGTGCCGGGATTATCTTCCGGGTGTATACATTTATGATATTCATAGCGAAACATGGCTCTCCAAACCAGTTCAGGGCCTCCCAAATGATGGGATCATCCTTCCCACCCTAGGCCTAAAAGAAGATGGTGAAAGTGTTTTTCACTCATCCCTGATGCAAGTCCGGAGAGATGAACTGAAATTGGCTCTGGTTTGGTCCCATGAGGTGCCCCGCGAACAGACTCAAGTTCATTGGTCCAAATTCTTACTCCACAGCAACAAAAACCACGGTGCCCCAGACTTCTATGCTGAACTCTTATCTCATGGAGTCTGTTTCGGTGGTGACACCCTTTTGCTCAATAGCACAACAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

235

Amino Acids

26.36

Weight (kDa)

5.68

Isoelectric Point (pI)

31.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 559, 623
AccB7I CCANNNNNTGG 1 cut(s) 443
AccI GTMKAC 1 cut(s) 381
AccII CGCG 2 cut(s) 228, 567
AccIII TCCGGA 1 cut(s) 516
AciI CCGC 5 cut(s) 88, 213, 226, 228, 565
AclWI GGATC 1 cut(s) 455
AcsI RAATTY 3 cut(s) 121, 268, 593
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 4 cut(s) 203, 428, 443, 464
AjnI CCWGG 1 cut(s) 113
Alw26I GTCTC 1 cut(s) 167
AlwI GGATC 1 cut(s) 455
Aor13HI TCCGGA 1 cut(s) 516
AoxI GGCC 3 cut(s) 85, 430, 466
ApoI RAATTY 3 cut(s) 121, 268, 593
AspA2I CCTAGG 1 cut(s) 463
AspLEI GCGC 1 cut(s) 345
AspS9I GGNCC 5 cut(s) 85, 288, 430, 549, 588
AsuC2I CCSGG 2 cut(s) 362, 375
AsuHPI GGTGA 2 cut(s) 494, 686
AvaII GGWCC 3 cut(s) 288, 549, 588
AvrII CCTAGG 1 cut(s) 463
BaeGI GKGCMC 2 cut(s) 564, 628
BanI GGYRCC 2 cut(s) 559, 623
BbsI GAAGAC 1 cut(s) 260
BccI CCATC 2 cut(s) 437, 473
BciT130I CCWGG 1 cut(s) 115
BcnI CCSGG 2 cut(s) 362, 375
BcoDI GTCTC 1 cut(s) 167
BfaI CTAG 1 cut(s) 464
BisI GCNGC 2 cut(s) 88, 229
BlnI CCTAGG 1 cut(s) 463
BlsI GCNGC 2 cut(s) 89, 230
Bme1390I CCNGG 3 cut(s) 115, 362, 375
Bme18I GGWCC 3 cut(s) 288, 549, 588
BmgT120I GGNCC 5 cut(s) 85, 288, 430, 549, 588
BmiI GGNNCC 3 cut(s) 551, 561, 625
BmrFI CCNGG 3 cut(s) 115, 362, 375
BmsI GCATC 2 cut(s) 139, 498
BpiI GAAGAC 1 cut(s) 260
BpmI CTGGAG 1 cut(s) 359
BpuEI CTTGAG 1 cut(s) 561
BpuMI CCSGG 2 cut(s) 362, 375
BsaI GGTCTC 1 cut(s) 167
BsaJI CCNNGG 5 cut(s) 48, 177, 226, 463, 619
BsaWI WCCGGW 1 cut(s) 516
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 428, 443, 464
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 1 cut(s) 422
BseAI TCCGGA 1 cut(s) 516
BseBI CCWGG 1 cut(s) 115
BseDI CCNNGG 5 cut(s) 48, 177, 226, 463, 619
BseGI GGATG 4 cut(s) 66, 106, 450, 500
BseLI CCNNNNNNNGG 4 cut(s) 203, 428, 443, 464
BseMII CTCAG 1 cut(s) 162
BseNI ACTGG 1 cut(s) 422
BseSI GKGCMC 2 cut(s) 564, 628
Bsh1236I CGCG 2 cut(s) 228, 567
BshFI GGCC 3 cut(s) 87, 432, 468
BshNI GGYRCC 2 cut(s) 559, 623
BsiSI CCGG 5 cut(s) 24, 294, 361, 374, 517
BslFI GGGAC 1 cut(s) 535
BslI CCNNNNNNNGG 4 cut(s) 203, 428, 443, 464
BsmAI GTCTC 1 cut(s) 167
BsmFI GGGAC 1 cut(s) 535
BsnI GGCC 3 cut(s) 87, 432, 468
Bso31I GGTCTC 1 cut(s) 167
Bsp1286I GDGCHC 2 cut(s) 564, 628
Bsp13I TCCGGA 1 cut(s) 516
Bsp1407I TGTACA 1 cut(s) 63
Bsp143I GATC 2 cut(s) 232, 447
BspACI CCGC 5 cut(s) 88, 213, 226, 228, 565
BspANI GGCC 3 cut(s) 87, 432, 468
BspCNI CTCAG 1 cut(s) 163
BspEI TCCGGA 1 cut(s) 516
BspFNI CGCG 2 cut(s) 228, 567
BspLI GGNNCC 3 cut(s) 551, 561, 625
BspPI GGATC 1 cut(s) 455
BspT107I GGYRCC 2 cut(s) 559, 623
BspTNI GGTCTC 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 23
BsrGI TGTACA 1 cut(s) 63
BsrI ACTGG 1 cut(s) 422
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 5 cut(s) 48, 177, 226, 463, 619
BssMI GATC 2 cut(s) 232, 447
BssNAI GTATAC 1 cut(s) 382
BssT1I CCWWGG 2 cut(s) 48, 463
Bst1107I GTATAC 1 cut(s) 382
Bst2UI CCWGG 1 cut(s) 115
Bst4CI ACNGT 4 cut(s) 56, 70, 248, 623
BstAPI GCANNNNNTGC 1 cut(s) 357
BstAUI TGTACA 1 cut(s) 63
BstC8I GCNNGC 3 cut(s) 131, 162, 353
BstDEI CTNAG 1 cut(s) 171
BstDSI CCRYGG 2 cut(s) 226, 619
BstF5I GGATG 4 cut(s) 66, 106, 450, 500
BstFNI CGCG 2 cut(s) 228, 567
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 2 cut(s) 235, 450
BstMAI GTCTC 1 cut(s) 167
BstMBI GATC 2 cut(s) 232, 447
BstMWI GCNNNNNNNGC 2 cut(s) 20, 357
BstNI CCWGG 1 cut(s) 115
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 3 cut(s) 113, 360, 373
BstSLI GKGCMC 2 cut(s) 564, 628
BstUI CGCG 2 cut(s) 228, 567
BstV2I GAAGAC 1 cut(s) 260
BstZ17I GTATAC 1 cut(s) 382
BsuRI GGCC 3 cut(s) 87, 432, 468
BtgI CCRYGG 2 cut(s) 226, 619
BtsCI GGATG 4 cut(s) 66, 106, 450, 500
BtsI GCAGTG 1 cut(s) 362
BtsIMutI CAGTG 1 cut(s) 362
Cac8I GCNNGC 3 cut(s) 131, 162, 353
CfoI GCGC 1 cut(s) 345
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 5 cut(s) 85, 288, 430, 549, 588
Cfr42I CCGCGG 1 cut(s) 229
Csp6I GTAC 1 cut(s) 64
CviAII CATG 4 cut(s) 352, 408, 554, 656
CviJI RGCY 5 cut(s) 87, 412, 432, 468, 539
CviKI_1 RGCY 5 cut(s) 87, 412, 432, 468, 539
CviQI GTAC 1 cut(s) 64
DdeI CTNAG 1 cut(s) 171
DpnI GATC 2 cut(s) 234, 449
DpnII GATC 2 cut(s) 232, 447
Eco130I CCWWGG 2 cut(s) 48, 463
Eco147I AGGCCT 1 cut(s) 468
Eco31I GGTCTC 1 cut(s) 167
Eco47I GGWCC 3 cut(s) 288, 549, 588
EcoO109I RGGNCCY 1 cut(s) 430
EcoRI GAATTC 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 113
EcoT14I CCWWGG 2 cut(s) 48, 463
ErhI CCWWGG 2 cut(s) 48, 463
FaeI CATG 4 cut(s) 355, 411, 557, 659
FaqI GGGAC 1 cut(s) 535
FatI CATG 4 cut(s) 351, 407, 553, 655
FauI CCCGC 1 cut(s) 572
FblI GTMKAC 1 cut(s) 381
Fnu4HI GCNGC 2 cut(s) 88, 229
FokI GGATG 4 cut(s) 73, 113, 437, 487
Fsp4HI GCNGC 2 cut(s) 88, 229
FspBI CTAG 1 cut(s) 464
GlaI GCGC 1 cut(s) 344
GluI GCNGC 2 cut(s) 88, 229
GsuI CTGGAG 1 cut(s) 359
HaeIII GGCC 3 cut(s) 87, 432, 468
HapII CCGG 5 cut(s) 24, 294, 361, 374, 517
HhaI GCGC 1 cut(s) 345
Hin1II CATG 4 cut(s) 355, 411, 557, 659
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HinfI GANTC 2 cut(s) 574, 660
HpaII CCGG 5 cut(s) 24, 294, 361, 374, 517
HphI GGTGA 2 cut(s) 494, 686
Hpy166II GTNNAC 3 cut(s) 66, 184, 382
Hpy188I TCNGA 3 cut(s) 148, 237, 267
Hpy188III TCNNGA 2 cut(s) 338, 517
Hpy8I GTNNAC 3 cut(s) 66, 184, 382
HpyAV CCTTC 2 cut(s) 207, 464
HpyCH4III ACNGT 4 cut(s) 56, 70, 248, 623
HpyCH4V TGCA 3 cut(s) 152, 355, 511
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 357
HpyF3I CTNAG 1 cut(s) 171
Hsp92II CATG 4 cut(s) 355, 411, 557, 659
HspAI GCGC 1 cut(s) 343
Kpn2I TCCGGA 1 cut(s) 516
KspI CCGCGG 1 cut(s) 229
Kzo9I GATC 2 cut(s) 232, 447
LmnI GCTCC 2 cut(s) 38, 340
LweI GCATC 2 cut(s) 139, 498
MaeI CTAG 1 cut(s) 464
MaeIII GTNAC 2 cut(s) 166, 674
MalI GATC 2 cut(s) 234, 449
MboI GATC 2 cut(s) 232, 447
MboII GAAGA 3 cut(s) 265, 362, 488
MhlI GDGCHC 2 cut(s) 564, 628
MluCI AATT 5 cut(s) 121, 268, 313, 533, 593
MlyI GAGTC 2 cut(s) 568, 669
MmeI TCCRAC 2 cut(s) 38, 230
MnlI CCTC 5 cut(s) 28, 172, 301, 443, 550
MroI TCCGGA 1 cut(s) 516
MseI TTAA 2 cut(s) 138, 261
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 5 cut(s) 24, 294, 361, 374, 517
MspR9I CCNGG 3 cut(s) 115, 362, 375
MvaI CCWGG 1 cut(s) 115
MvnI CGCG 2 cut(s) 228, 567
MwoI GCNNNNNNNGC 2 cut(s) 20, 357
NciI CCSGG 2 cut(s) 362, 375
NdeII GATC 2 cut(s) 232, 447
NlaIII CATG 4 cut(s) 355, 411, 557, 659
NlaIV GGNNCC 3 cut(s) 551, 561, 625
NmuCI GTSAC 1 cut(s) 674
NspI RCATGY 1 cut(s) 355
PaeI GCATGC 1 cut(s) 355
PceI AGGCCT 1 cut(s) 468
PflMI CCANNNNNTGG 1 cut(s) 443
PkrI GCNGC 2 cut(s) 89, 230
PleI GAGTC 2 cut(s) 568, 668
PpsI GAGTC 2 cut(s) 568, 668
Psp6I CCWGG 1 cut(s) 113
PspGI CCWGG 1 cut(s) 113
PspN4I GGNNCC 3 cut(s) 551, 561, 625
PspPI GGNCC 5 cut(s) 85, 288, 430, 549, 588
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
SacII CCGCGG 1 cut(s) 229
SaqAI TTAA 2 cut(s) 138, 261
SatI GCNGC 2 cut(s) 88, 229
Sau3AI GATC 2 cut(s) 232, 447
Sau96I GGNCC 5 cut(s) 85, 288, 430, 549, 588
SchI GAGTC 2 cut(s) 568, 669
ScrFI CCNGG 3 cut(s) 115, 362, 375
SduI GDGCHC 2 cut(s) 564, 628
SetI ASST 5 cut(s) 116, 159, 183, 189, 561
SfaNI GCATC 2 cut(s) 139, 498
Sfr303I CCGCGG 1 cut(s) 229
SgrAI CRCCGGYG 1 cut(s) 23
SgrBI CCGCGG 1 cut(s) 229
SinI GGWCC 3 cut(s) 288, 549, 588
SmlI CTYRAG 1 cut(s) 576
SmoI CTYRAG 1 cut(s) 576
SphI GCATGC 1 cut(s) 355
Sse9I AATT 5 cut(s) 121, 268, 313, 533, 593
SseBI AGGCCT 1 cut(s) 468
SsiI CCGC 5 cut(s) 88, 213, 226, 228, 565
SspMI CTAG 1 cut(s) 464
StuI AGGCCT 1 cut(s) 468
StyD4I CCNGG 3 cut(s) 113, 360, 373
StyI CCWWGG 2 cut(s) 48, 463
TaaI ACNGT 4 cut(s) 56, 70, 248, 623
TaqII GACCGA 1 cut(s) 191
TasI AATT 5 cut(s) 121, 268, 313, 533, 593
TatI WGTACW 1 cut(s) 63
TauI GCSGC 2 cut(s) 90, 231
Tru1I TTAA 2 cut(s) 138, 261
Tru9I TTAA 2 cut(s) 138, 261
TscAI CASTG 1 cut(s) 362
TseFI GTSAC 1 cut(s) 674
Tsp45I GTSAC 1 cut(s) 674
TspDTI ATGAA 4 cut(s) 189, 386, 540, 572
TspGWI ACGGA 3 cut(s) 83, 132, 264
TspRI CASTG 1 cut(s) 362
Van91I CCANNNNNTGG 1 cut(s) 443
VpaK11BI GGWCC 3 cut(s) 288, 549, 588
XapI RAATTY 3 cut(s) 121, 268, 593
XceI RCATGY 1 cut(s) 355
XmaJI CCTAGG 1 cut(s) 463
XmiI GTMKAC 1 cut(s) 381
XspI CTAG 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.