Rorug02G0266200

THO complex subunit 4A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004491
Physical Location & Seq
Forward (+)
28157911 .. 28162090
4180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0266200.1

Sequence Viewer

Length: 636 bp
ATGGGGAAGGAGGAGATCACAGTAGTTCTCAGGCTTGGTTTCACCATTCGATGGTTTCAGCGGCGGACTAGTTCGGTGTTGGGGGATCGGAGGTCTGACGGTGACATGCTTGTGGTGGCATGTTGTCGTACCGTTCGGAGGTTGGTCAGTGGTGGCATGGCTTGGAGGTACAGCTTTCTGCCAATCGGATCAATCCGTTCTGGGTTGGAACCCTTGGATCCGACCTGGGATTGGGTTCGTCTGCTTGGGGCGGTGATTATTCATGGTTTGTCTGGAAAGGCAGTGGGTTCAAGTCGGCGGTCCTGCGAATGCGGTGTGGTGCGGTGGTCTGGCGACGGAGGTTGGGGCGGGGATGCGAGGCATGGCAGGCTTCTTGGGGTCGTCCTCTCTCCTGGGTCAAAGCTCGGGCCCGGCATAGATGGGTATGAACTAGCTGTCCAGATTCATGAAAAGTTTACAAAACGTCATGAACGACCAGTATTAGTTGCACTTACTGGAAACATTAACAAGATGACAAAGGAGAGCTGCATGAGGGTTGGTATGGACGGAGTTATACTAAAACCTGTTTCAGTTGATAAACTGAGAAGTGTTTTATCTGAACTACTAGAGCATCGGGTTCTATTTGAGGCAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

211

Amino Acids

23.25

Weight (kDa)

9.84

Isoelectric Point (pI)

61.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 137 - 197 3.3e-06 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 51
AciI CCGC 7 cut(s) 61, 64, 251, 298, 312, 322, 348
AclWI GGATC 4 cut(s) 93, 196, 212, 225
AfaI GTAC 2 cut(s) 130, 170
AfiI CCNNNNNNNGG 3 cut(s) 51, 138, 231
AgsI TTSAA 1 cut(s) 291
AhlI ACTAGT 1 cut(s) 68
AjnI CCWGG 2 cut(s) 224, 391
AloI GAACNNNNNNTCC 2 cut(s) 420, 452
AluBI AGCT 4 cut(s) 174, 403, 434, 525
AluI AGCT 4 cut(s) 174, 403, 434, 525
AlwI GGATC 4 cut(s) 93, 196, 212, 225
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 1 cut(s) 407
ApaI GGGCCC 1 cut(s) 411
ApeKI GCWGC 1 cut(s) 525
AspS9I GGNCC 3 cut(s) 300, 407, 408
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 3 cut(s) 34, 113, 265
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 1 cut(s) 300
BaeGI GKGCMC 1 cut(s) 411
BamHI GGATCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 411
BbvI GCAGC 1 cut(s) 512
BccI CCATC 2 cut(s) 45, 413
BciT130I CCWGG 2 cut(s) 226, 393
BcnI CCSGG 1 cut(s) 411
BcuI ACTAGT 1 cut(s) 68
BfaI CTAG 3 cut(s) 69, 431, 605
BisI GCNGC 2 cut(s) 62, 526
BlsI GCNGC 2 cut(s) 63, 527
Bme1390I CCNGG 3 cut(s) 226, 393, 411
Bme18I GGWCC 1 cut(s) 300
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 3 cut(s) 300, 407, 408
BmiI GGNNCC 3 cut(s) 210, 219, 409
BmrFI CCNGG 3 cut(s) 226, 393, 411
BmsI GCATC 2 cut(s) 343, 619
BpuMI CCSGG 1 cut(s) 411
BsaJI CCNNGG 3 cut(s) 213, 225, 392
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 138, 231
Bse1I ACTGG 2 cut(s) 476, 499
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 2 cut(s) 226, 393
BseDI CCNNGG 3 cut(s) 213, 225, 392
BseGI GGATG 1 cut(s) 358
BseLI CCNNNNNNNGG 3 cut(s) 51, 138, 231
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 2 cut(s) 43, 572
BseNI ACTGG 2 cut(s) 476, 499
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 1 cut(s) 411
BseXI GCAGC 1 cut(s) 512
BshFI GGCC 1 cut(s) 409
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 1 cut(s) 411
BslI CCNNNNNNNGG 3 cut(s) 51, 138, 231
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 1 cut(s) 409
BsoBI CYCGRG 1 cut(s) 404
Bsp120I GGGCCC 1 cut(s) 407
Bsp1286I GDGCHC 1 cut(s) 411
Bsp143I GATC 4 cut(s) 15, 85, 188, 217
BspACI CCGC 7 cut(s) 61, 64, 251, 298, 312, 322, 348
BspANI GGCC 1 cut(s) 409
BspCNI CTCAG 2 cut(s) 42, 573
BspHI TCATGA 2 cut(s) 445, 466
BspLI GGNNCC 3 cut(s) 210, 219, 409
BspPI GGATC 4 cut(s) 93, 196, 212, 225
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 2 cut(s) 476, 499
BssECI CCNNGG 3 cut(s) 213, 225, 392
BssMI GATC 4 cut(s) 15, 85, 188, 217
BssT1I CCWWGG 1 cut(s) 213
Bst2UI CCWGG 2 cut(s) 226, 393
Bst4CI ACNGT 3 cut(s) 22, 101, 133
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 2 cut(s) 29, 581
BstF5I GGATG 1 cut(s) 358
BstKTI GATC 4 cut(s) 18, 88, 191, 220
BstMBI GATC 4 cut(s) 15, 85, 188, 217
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstNI CCWGG 2 cut(s) 226, 393
BstNSI RCATGY 2 cut(s) 109, 123
BstSCI CCNGG 3 cut(s) 224, 391, 409
BstSLI GKGCMC 1 cut(s) 411
BstV1I GCAGC 1 cut(s) 512
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuRI GGCC 1 cut(s) 409
BtsCI GGATG 1 cut(s) 358
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 2 cut(s) 154, 288
Cac8I GCNNGC 1 cut(s) 368
CciI TCATGA 2 cut(s) 445, 466
Cfr13I GGNCC 3 cut(s) 300, 407, 408
Csp6I GTAC 2 cut(s) 129, 169
CviAII CATG 8 cut(s) 106, 120, 157, 263, 362, 446, 467, 529
CviJI RGCY 8 cut(s) 34, 161, 174, 370, 403, 409, 434, 525
CviKI_1 RGCY 8 cut(s) 34, 161, 174, 370, 403, 409, 434, 525
CviQI GTAC 2 cut(s) 129, 169
DdeI CTNAG 2 cut(s) 29, 581
DpnI GATC 4 cut(s) 17, 87, 190, 219
DpnII GATC 4 cut(s) 15, 85, 188, 217
EciI GGCGGA 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 411
Eco47I GGWCC 1 cut(s) 300
Eco88I CYCGRG 1 cut(s) 404
EcoRII CCWGG 2 cut(s) 224, 391
EcoT14I CCWWGG 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 411
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 8 cut(s) 109, 123, 160, 266, 365, 449, 470, 532
FatI CATG 8 cut(s) 105, 119, 156, 262, 361, 445, 466, 528
FauI CCCGC 1 cut(s) 341
Fnu4HI GCNGC 2 cut(s) 62, 526
FokI GGATG 1 cut(s) 365
FriOI GRGCYC 1 cut(s) 411
Fsp4HI GCNGC 2 cut(s) 62, 526
FspBI CTAG 3 cut(s) 69, 431, 605
GluI GCNGC 2 cut(s) 62, 526
HaeIII GGCC 1 cut(s) 409
HapII CCGG 1 cut(s) 411
Hin1II CATG 8 cut(s) 109, 123, 160, 266, 365, 449, 470, 532
HinfI GANTC 1 cut(s) 442
HpaII CCGG 1 cut(s) 411
HphI GGTGA 3 cut(s) 34, 113, 265
Hpy166II GTNNAC 1 cut(s) 456
Hpy188I TCNGA 6 cut(s) 90, 97, 138, 188, 222, 598
Hpy188III TCNNGA 4 cut(s) 273, 439, 446, 467
Hpy8I GTNNAC 1 cut(s) 456
Hpy99I CGWCG 1 cut(s) 338
HpyCH4III ACNGT 3 cut(s) 22, 101, 133
HpyCH4IV ACGT 1 cut(s) 463
HpyCH4V TGCA 2 cut(s) 488, 528
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpyF3I CTNAG 2 cut(s) 29, 581
HpySE526I ACGT 1 cut(s) 463
Hsp92II CATG 8 cut(s) 109, 123, 160, 266, 365, 449, 470, 532
Kzo9I GATC 4 cut(s) 15, 85, 188, 217
Lsp1109I GCAGC 1 cut(s) 512
LweI GCATC 2 cut(s) 343, 619
MaeI CTAG 3 cut(s) 69, 431, 605
MaeII ACGT 1 cut(s) 463
MaeIII GTNAC 1 cut(s) 101
MalI GATC 4 cut(s) 17, 87, 190, 219
MboI GATC 4 cut(s) 15, 85, 188, 217
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 1 cut(s) 411
MmeI TCCRAC 2 cut(s) 186, 245
MnlI CCTC 9 cut(s) 4, 84, 132, 159, 332, 351, 395, 525, 619
MseI TTAA 1 cut(s) 504
MslI CAYNNNNRTG 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 1 cut(s) 411
MspR9I CCNGG 3 cut(s) 226, 393, 411
Mva1269I GAATGC 1 cut(s) 314
MvaI CCWGG 2 cut(s) 226, 393
MwoI GCNNNNNNNGC 1 cut(s) 367
NciI CCSGG 1 cut(s) 411
NdeII GATC 4 cut(s) 15, 85, 188, 217
NlaIII CATG 8 cut(s) 109, 123, 160, 266, 365, 449, 470, 532
NlaIV GGNNCC 3 cut(s) 210, 219, 409
NmuCI GTSAC 1 cut(s) 101
NspI RCATGY 2 cut(s) 109, 123
PagI TCATGA 2 cut(s) 445, 466
PcsI WCGNNNNNNNCGW 1 cut(s) 469
PctI GAATGC 1 cut(s) 314
PfeI GAWTC 1 cut(s) 442
PflMI CCANNNNNTGG 1 cut(s) 51
PkrI GCNGC 2 cut(s) 63, 527
Psp6I CCWGG 2 cut(s) 224, 391
PspGI CCWGG 2 cut(s) 224, 391
PspN4I GGNNCC 3 cut(s) 210, 219, 409
PspOMI GGGCCC 1 cut(s) 407
PspPI GGNCC 3 cut(s) 300, 407, 408
PsuI RGATCY 1 cut(s) 217
RsaI GTAC 2 cut(s) 130, 170
RsaNI GTAC 2 cut(s) 129, 169
RseI CAYNNNNRTG 1 cut(s) 110
SaqAI TTAA 1 cut(s) 504
SatI GCNGC 2 cut(s) 62, 526
Sau3AI GATC 4 cut(s) 15, 85, 188, 217
Sau96I GGNCC 3 cut(s) 300, 407, 408
ScrFI CCNGG 3 cut(s) 226, 393, 411
SduI GDGCHC 1 cut(s) 411
SfaNI GCATC 2 cut(s) 343, 619
SinI GGWCC 1 cut(s) 300
SmiMI CAYNNNNRTG 1 cut(s) 110
SpeI ACTAGT 1 cut(s) 68
SsiI CCGC 7 cut(s) 61, 64, 251, 298, 312, 322, 348
SspMI CTAG 3 cut(s) 69, 431, 605
StyD4I CCNGG 3 cut(s) 224, 391, 409
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 3 cut(s) 22, 101, 133
TaiI ACGT 1 cut(s) 466
TaqI TCGA 1 cut(s) 49
TauI GCSGC 1 cut(s) 64
TfiI GAWTC 1 cut(s) 442
Tru1I TTAA 1 cut(s) 504
Tru9I TTAA 1 cut(s) 504
TscAI CASTG 2 cut(s) 154, 288
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 1 cut(s) 525
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 5 cut(s) 251, 434, 441, 462, 483
TspGWI ACGGA 3 cut(s) 185, 351, 561
TspRI CASTG 2 cut(s) 154, 288
Van91I CCANNNNNTGG 1 cut(s) 51
VpaK11BI GGWCC 1 cut(s) 300
XceI RCATGY 2 cut(s) 109, 123
XspI CTAG 3 cut(s) 69, 431, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.