RLG00000027356

THO complex subunit 4A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004491
Physical Location & Seq
Reverse (-)
10878714 .. 10881399
2686 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027356

Sequence Viewer

Length: 852 bp
ATGCTCCTCCCTCACGACGCCCTCATCGCCGAATCCAAGCAACAACGCCGTCACAACAACCAGGCTCACCACCCACGAGGGCATACCGGTCATTTCGGCGGCAGAGGCCGTGGAGGCTCCACCTCGGGCCCCGCTCGTCGGCCGATGAACCGCAACTCCTACAGAACGGCGCCGTATCCTACTCTGCAGGAAATCCAAGGACAGCCTGGACCCAGAAGGATTGAGCTGCCATTGGCTTTGAGCCAAGCGATGAACAGAGAGGGAAGCACGAAGCTTTATATATCTAACTTGGACTATGATGTTACCAATAGAGATATAGAGTTGCTCTTCTCTGAGGTTGGTGAGTTGGAAAGGCACTCAATCCATTATGATAAAAGTGGAAGATCTAAGGGAACAGCTGAAGTTGTCTATAAGCACCATTCAGATGCTCTAACAGCAATTGAGAGATATAACAATCAGCATCTTGATGGAAAGAGAGTGGAAATTAAGCTTGTGGGACTCAATGTTGTTTCTCCTGTTCCTCTGCTTCTGCCTCCAAATACAAATTTCTTGCAAGAAAATCCAAGTCCTGTCTTCCTGAGGCTAGGAAGAGCCGGTTCAAGGGGATCGTTTCATGGTCATGGTGGTGTTGGACTTGGAAGAGGCCGTGGACAGGTGAGAAATGGCGGTCTGAAGGTTACTCCAGAATCTCAGCAAGGGAAGAATGTGACTACAGAAAATCAGCAAAGGAAGAATGTGAATACAGAATCTCATAACAGGAAGAAAACTTTTTATGGAGATGCAACTGTGACTTTTGAAGATCTGGATGCTGATTTGGAGAAATACCGCCTAAAAGGTAGGCTGGTAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

284

Amino Acids

31.57

Weight (kDa)

10.02

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 92 - 160 1.9e-15 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 169
AccBSI CCGCTC 1 cut(s) 134
AciI CCGC 5 cut(s) 99, 132, 151, 666, 826
AclWI GGATC 1 cut(s) 613
AcoI YGGCCR 1 cut(s) 140
AcsI RAATTY 1 cut(s) 544
AcuI CTGAAG 2 cut(s) 420, 692
AcyI GRCGYC 2 cut(s) 18, 170
AfiI CCNNNNNNNGG 3 cut(s) 138, 600, 652
AgeI ACCGGT 1 cut(s) 86
AgsI TTSAA 2 cut(s) 600, 797
AjnI CCWGG 2 cut(s) 60, 205
AloI GAACNNNNNNTCC 2 cut(s) 140, 172
AluBI AGCT 4 cut(s) 226, 274, 398, 490
AluI AGCT 4 cut(s) 226, 274, 398, 490
AlwI GGATC 1 cut(s) 613
Ama87I CYCGRG 1 cut(s) 124
AoxI GGCC 4 cut(s) 106, 127, 140, 643
ApaI GGGCCC 1 cut(s) 131
ApeKI GCWGC 1 cut(s) 226
ApoI RAATTY 1 cut(s) 544
AsiGI ACCGGT 1 cut(s) 86
AspLEI GCGC 1 cut(s) 172
AspS9I GGNCC 3 cut(s) 127, 128, 209
AsuHPI GGTGA 3 cut(s) 59, 353, 667
AvaI CYCGRG 1 cut(s) 124
AvaII GGWCC 1 cut(s) 209
AxyI CCTNAGG 1 cut(s) 578
BaeGI GKGCMC 1 cut(s) 131
BanI GGYRCC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 131
BauI CACGAG 1 cut(s) 75
BbsI GAAGAC 1 cut(s) 565
BbvI GCAGC 1 cut(s) 213
BccI CCATC 1 cut(s) 461
BceAI ACGGC 5 cut(s) 33, 93, 157, 183, 630
BciT130I CCWGG 2 cut(s) 62, 207
BciVI GTATCC 1 cut(s) 186
BfaI CTAG 1 cut(s) 584
BfmI CTRYAG 3 cut(s) 160, 185, 711
BfoI RGCGCY 1 cut(s) 173
BfuI GTATCC 1 cut(s) 186
BglI GCCNNNNNGGC 1 cut(s) 114
BglII AGATCT 2 cut(s) 383, 799
BisI GCNGC 2 cut(s) 100, 227
BlsI GCNGC 2 cut(s) 101, 228
Bme1390I CCNGG 2 cut(s) 62, 207
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 124
BmgT120I GGNCC 3 cut(s) 127, 128, 209
BmiI GGNNCC 5 cut(s) 118, 129, 130, 171, 211
BmrFI CCNGG 2 cut(s) 62, 207
BmsI GCATC 4 cut(s) 415, 469, 769, 796
BpiI GAAGAC 1 cut(s) 565
BpmI CTGGAG 1 cut(s) 666
BsaHI GRCGYC 2 cut(s) 18, 170
BsaJI CCNNGG 4 cut(s) 109, 123, 196, 646
BsaWI WCCGGW 1 cut(s) 86
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 600, 652
Bse118I RCCGGY 2 cut(s) 86, 593
Bse21I CCTNAGG 1 cut(s) 578
BseBI CCWGG 2 cut(s) 62, 207
BseDI CCNNGG 4 cut(s) 109, 123, 196, 646
BseGI GGATG 1 cut(s) 811
BseLI CCNNNNNNNGG 3 cut(s) 138, 600, 652
BseMII CTCAG 3 cut(s) 324, 569, 704
BseSI GKGCMC 1 cut(s) 131
BseX3I CGGCCG 1 cut(s) 140
BseXI GCAGC 1 cut(s) 213
Bsh1285I CGRYCG 1 cut(s) 143
BshFI GGCC 4 cut(s) 108, 129, 142, 645
BshNI GGYRCC 1 cut(s) 169
BshTI ACCGGT 1 cut(s) 86
BsiEI CGRYCG 1 cut(s) 143
BsiHKCI CYCGRG 1 cut(s) 124
BsiSI CCGG 2 cut(s) 87, 594
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 3 cut(s) 138, 600, 652
BsmFI GGGAC 1 cut(s) 510
BsnI GGCC 4 cut(s) 108, 129, 142, 645
BsoBI CYCGRG 1 cut(s) 124
Bsp120I GGGCCC 1 cut(s) 127
Bsp1286I GDGCHC 1 cut(s) 131
Bsp143I GATC 3 cut(s) 383, 605, 799
BspACI CCGC 5 cut(s) 99, 132, 151, 666, 826
BspANI GGCC 4 cut(s) 108, 129, 142, 645
BspCNI CTCAG 3 cut(s) 325, 570, 703
BspLI GGNNCC 5 cut(s) 118, 129, 130, 171, 211
BspMAI CTGCAG 1 cut(s) 189
BspPI GGATC 1 cut(s) 613
BspQI GCTCTTC 2 cut(s) 332, 583
BspT107I GGYRCC 1 cut(s) 169
BsrBI CCGCTC 1 cut(s) 134
BsrFI RCCGGY 2 cut(s) 86, 593
BssAI RCCGGY 2 cut(s) 86, 593
BssECI CCNNGG 4 cut(s) 109, 123, 196, 646
BssMI GATC 3 cut(s) 383, 605, 799
BssNI GRCGYC 2 cut(s) 18, 170
BssSI CACGAG 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 196
Bst2BI CACGAG 1 cut(s) 75
Bst2UI CCWGG 2 cut(s) 62, 207
Bst4CI ACNGT 1 cut(s) 787
Bst6I CTCTTC 3 cut(s) 332, 583, 634
BstACI GRCGYC 2 cut(s) 18, 170
BstDEI CTNAG 4 cut(s) 333, 387, 578, 690
BstDSI CCRYGG 2 cut(s) 109, 646
BstF5I GGATG 1 cut(s) 811
BstH2I RGCGCY 1 cut(s) 173
BstHHI GCGC 1 cut(s) 172
BstKTI GATC 3 cut(s) 386, 608, 802
BstMBI GATC 3 cut(s) 383, 605, 799
BstMCI CGRYCG 1 cut(s) 143
BstMWI GCNNNNNNNGC 4 cut(s) 26, 105, 114, 434
BstNI CCWGG 2 cut(s) 62, 207
BstSCI CCNGG 2 cut(s) 60, 205
BstSFI CTRYAG 3 cut(s) 160, 185, 711
BstSLI GKGCMC 1 cut(s) 131
BstV1I GCAGC 1 cut(s) 213
BstV2I GAAGAC 1 cut(s) 565
BstX2I RGATCY 2 cut(s) 383, 799
BstYI RGATCY 2 cut(s) 383, 799
BstZI CGGCCG 1 cut(s) 140
Bsu36I CCTNAGG 1 cut(s) 578
BsuI GTATCC 1 cut(s) 186
BsuRI GGCC 4 cut(s) 108, 129, 142, 645
BtgI CCRYGG 2 cut(s) 109, 646
BtgZI GCGATG 2 cut(s) 10, 263
BtsCI GGATG 1 cut(s) 811
CfoI GCGC 1 cut(s) 172
Cfr10I RCCGGY 2 cut(s) 86, 593
Cfr13I GGNCC 3 cut(s) 127, 128, 209
CseI GACGC 1 cut(s) 26
CspAI ACCGGT 1 cut(s) 86
CviAII CATG 2 cut(s) 614, 620
DdeI CTNAG 4 cut(s) 333, 387, 578, 690
DinI GGCGCC 1 cut(s) 171
DpnI GATC 3 cut(s) 385, 607, 801
DpnII GATC 3 cut(s) 383, 605, 799
EaeI YGGCCR 1 cut(s) 140
EagI CGGCCG 1 cut(s) 140
Eam1104I CTCTTC 3 cut(s) 332, 583, 634
EarI CTCTTC 3 cut(s) 332, 583, 634
EclXI CGGCCG 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 209
Eco52I CGGCCG 1 cut(s) 140
Eco57I CTGAAG 2 cut(s) 420, 692
Eco81I CCTNAGG 1 cut(s) 578
Eco88I CYCGRG 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 2 cut(s) 60, 205
EcoT14I CCWWGG 1 cut(s) 196
EcoT38I GRGCYC 1 cut(s) 131
EgeI GGCGCC 1 cut(s) 171
EheI GGCGCC 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 196
FaeI CATG 2 cut(s) 617, 623
FaqI GGGAC 1 cut(s) 510
FatI CATG 2 cut(s) 613, 619
FauI CCCGC 1 cut(s) 139
Fnu4HI GCNGC 2 cut(s) 100, 227
FokI GGATG 1 cut(s) 818
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 2 cut(s) 100, 227
FspBI CTAG 1 cut(s) 584
GlaI GCGC 1 cut(s) 171
GluI GCNGC 2 cut(s) 100, 227
GsuI CTGGAG 1 cut(s) 666
HaeII RGCGCY 1 cut(s) 173
HaeIII GGCC 4 cut(s) 108, 129, 142, 645
HapII CCGG 2 cut(s) 87, 594
HgaI GACGC 1 cut(s) 26
HhaI GCGC 1 cut(s) 172
Hin1I GRCGYC 2 cut(s) 18, 170
Hin1II CATG 2 cut(s) 617, 623
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HindIII AAGCTT 2 cut(s) 272, 488
HinfI GANTC 4 cut(s) 32, 498, 686, 746
HpaII CCGG 2 cut(s) 87, 594
HphI GGTGA 3 cut(s) 59, 353, 667
Hpy166II GTNNAC 2 cut(s) 650, 847
Hpy188I TCNGA 3 cut(s) 334, 424, 672
Hpy188III TCNNGA 5 cut(s) 14, 464, 577, 683, 803
Hpy8I GTNNAC 2 cut(s) 650, 847
Hpy99I CGWCG 2 cut(s) 20, 141
HpyAV CCTTC 2 cut(s) 210, 667
HpyCH4III ACNGT 1 cut(s) 787
HpyCH4V TGCA 3 cut(s) 187, 553, 782
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 105, 114, 434
HpyF3I CTNAG 4 cut(s) 333, 387, 578, 690
Hsp92I GRCGYC 2 cut(s) 18, 170
Hsp92II CATG 2 cut(s) 617, 623
HspAI GCGC 1 cut(s) 170
KasI GGCGCC 1 cut(s) 169
Kzo9I GATC 3 cut(s) 383, 605, 799
LguI GCTCTTC 2 cut(s) 332, 583
LmnI GCTCC 2 cut(s) 9, 122
Lsp1109I GCAGC 1 cut(s) 213
LweI GCATC 4 cut(s) 415, 469, 769, 796
MaeI CTAG 1 cut(s) 584
MaeIII GTNAC 5 cut(s) 50, 301, 676, 706, 787
MalI GATC 3 cut(s) 385, 607, 801
MbiI CCGCTC 1 cut(s) 134
MboI GATC 3 cut(s) 383, 605, 799
MboII GAAGA 9 cut(s) 319, 393, 565, 600, 651, 712, 742, 772, 809
MfeI CAATTG 1 cut(s) 438
MflI RGATCY 2 cut(s) 383, 799
MhlI GDGCHC 1 cut(s) 131
MluCI AATT 3 cut(s) 438, 483, 544
Mly113I GGCGCC 1 cut(s) 170
MlyI GAGTC 1 cut(s) 492
MmeI TCCRAC 2 cut(s) 327, 610
MseI TTAA 1 cut(s) 486
MslI CAYNNNNRTG 4 cut(s) 423, 465, 618, 624
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 2 cut(s) 87, 594
MspR9I CCNGG 2 cut(s) 62, 207
MunI CAATTG 1 cut(s) 438
MvaI CCWGG 2 cut(s) 62, 207
MwoI GCNNNNNNNGC 4 cut(s) 26, 105, 114, 434
NarI GGCGCC 1 cut(s) 170
NdeII GATC 3 cut(s) 383, 605, 799
NlaIII CATG 2 cut(s) 617, 623
NlaIV GGNNCC 5 cut(s) 118, 129, 130, 171, 211
NmuCI GTSAC 3 cut(s) 50, 706, 787
PciSI GCTCTTC 2 cut(s) 332, 583
PfeI GAWTC 3 cut(s) 32, 686, 746
PinAI ACCGGT 1 cut(s) 86
PkrI GCNGC 2 cut(s) 101, 228
PleI GAGTC 1 cut(s) 492
PluTI GGCGCC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 492
Psp6I CCWGG 2 cut(s) 60, 205
PspGI CCWGG 2 cut(s) 60, 205
PspN4I GGNNCC 5 cut(s) 118, 129, 130, 171, 211
PspOMI GGGCCC 1 cut(s) 127
PspPI GGNCC 3 cut(s) 127, 128, 209
PstI CTGCAG 1 cut(s) 189
PsuI RGATCY 2 cut(s) 383, 799
PvuII CAGCTG 1 cut(s) 398
RseI CAYNNNNRTG 4 cut(s) 423, 465, 618, 624
SapI GCTCTTC 2 cut(s) 332, 583
SaqAI TTAA 1 cut(s) 486
SatI GCNGC 2 cut(s) 100, 227
Sau3AI GATC 3 cut(s) 383, 605, 799
Sau96I GGNCC 3 cut(s) 127, 128, 209
SchI GAGTC 1 cut(s) 492
ScrFI CCNGG 2 cut(s) 62, 207
SduI GDGCHC 1 cut(s) 131
SetI ASST 9 cut(s) 125, 228, 276, 339, 400, 492, 657, 678, 838
SfaNI GCATC 4 cut(s) 415, 469, 769, 796
SfcI CTRYAG 3 cut(s) 160, 185, 711
SfoI GGCGCC 1 cut(s) 171
SinI GGWCC 1 cut(s) 209
SmiMI CAYNNNNRTG 4 cut(s) 423, 465, 618, 624
Sse9I AATT 3 cut(s) 438, 483, 544
SsiI CCGC 5 cut(s) 99, 132, 151, 666, 826
SspDI GGCGCC 1 cut(s) 169
SspMI CTAG 1 cut(s) 584
StyD4I CCNGG 2 cut(s) 60, 205
StyI CCWWGG 1 cut(s) 196
TaaI ACNGT 1 cut(s) 787
TasI AATT 3 cut(s) 438, 483, 544
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 3 cut(s) 32, 686, 746
Tru1I TTAA 1 cut(s) 486
Tru9I TTAA 1 cut(s) 486
TseFI GTSAC 3 cut(s) 50, 706, 787
TseI GCWGC 1 cut(s) 226
Tsp45I GTSAC 3 cut(s) 50, 706, 787
TspDTI ATGAA 3 cut(s) 161, 266, 602
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 1 cut(s) 544
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XspI CTAG 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.