Rw3G026530

THO complex subunit 4A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0004491
Physical Location & Seq
Reverse (-)
36445392 .. 36454815
9424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G026530.1

Sequence Viewer

Length: 663 bp
ATGCCCTTCCCTCAGGACCGCCTGGACCTCCCTCTCGACGCCCTCATCGCCGAATCCAAGCAACAACGCCGTCACAACAGCCACGCTCACCAGCCACGAGGGCATAACGGTCATTTCGGCGGCGGAGGCTCCACCTCAGGCCCCTCTCGTCGGCCGATGAACCGCAACGCCTACAGAACGGCGCCGTATCCCACTCTGCCGGAAATGCAAGGACAGCCTGGACCCAGAAGGATTGAGCTGCCATTGGCTTTGAGCCAAGCGATGAACAGTGAGGGAAGCACCAAGCTTTATATATCTAACTTGGACTATGATGTTACCAATCGAGATATAGAGTTGCTCTTCTCTGAGGTTGGTGAATTGGAAAGGCACTCAATCCATTATGATAAAAGTGGAAGATCTAAGGGAACAGCTGAAGTTGTCTATAAGCACTATTCAGATGCTCTAGCAGCAATCGAGAGATACAACAATCAGCATCTTGATGGAAAGAAAGTGGAAATTAAGCTTGTGGGACTCAATGTTGTTTCTCCTGTTCCTCTGCTTCTGCCTCCAAATACAAATTTCATGCAAGAACATCCAACTCCCGTCTTCCAGAGGTATAGTACTTTTGATGATCTGGATGCTGATTTGGAAAAATACCGCCTAAAATGTAGGCCGGTAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05168 map03013 map03015 map03040 map05168
Pfam Domains
Protein Families
FoP_duplication RRM_1

Protein Analysis

220

Amino Acids

24.88

Weight (kDa)

7.9

Isoelectric Point (pI)

54.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 96 - 164 6.3e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 181
AciI CCGC 5 cut(s) 19, 120, 123, 163, 637
AcoI YGGCCR 1 cut(s) 152
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 1 cut(s) 432
AcyI GRCGYC 2 cut(s) 39, 182
AfaI GTAC 1 cut(s) 601
AfiI CCNNNNNNNGG 1 cut(s) 150
AjnI CCWGG 2 cut(s) 21, 217
AluBI AGCT 4 cut(s) 238, 286, 410, 502
AluI AGCT 4 cut(s) 238, 286, 410, 502
AoxI GGCC 3 cut(s) 139, 152, 650
ApeKI GCWGC 2 cut(s) 238, 446
ApoI RAATTY 1 cut(s) 556
AspLEI GCGC 1 cut(s) 184
AspS9I GGNCC 4 cut(s) 16, 25, 140, 221
AsuHPI GGTGA 2 cut(s) 80, 365
AvaII GGWCC 3 cut(s) 16, 25, 221
AxyI CCTNAGG 2 cut(s) 12, 136
BanI GGYRCC 1 cut(s) 181
BauI CACGAG 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 577
BbvI GCAGC 2 cut(s) 225, 458
BccI CCATC 1 cut(s) 473
BceAI ACGGC 3 cut(s) 54, 169, 195
BciT130I CCWGG 2 cut(s) 23, 219
BciVI GTATCC 1 cut(s) 198
BfaI CTAG 1 cut(s) 443
BfmI CTRYAG 1 cut(s) 172
BfoI RGCGCY 1 cut(s) 185
BfuI GTATCC 1 cut(s) 198
BglI GCCNNNNNGGC 1 cut(s) 100
BglII AGATCT 1 cut(s) 395
BisI GCNGC 3 cut(s) 121, 239, 447
BlsI GCNGC 3 cut(s) 122, 240, 448
BmcAI AGTACT 1 cut(s) 601
Bme1390I CCNGG 2 cut(s) 23, 219
Bme18I GGWCC 3 cut(s) 16, 25, 221
BmgT120I GGNCC 4 cut(s) 16, 25, 140, 221
BmiI GGNNCC 4 cut(s) 130, 142, 183, 223
BmrFI CCNGG 2 cut(s) 23, 219
BmsI GCATC 3 cut(s) 427, 481, 607
BpiI GAAGAC 1 cut(s) 577
BsaHI GRCGYC 2 cut(s) 39, 182
Bsc4I CCNNNNNNNGG 1 cut(s) 150
Bse118I RCCGGY 1 cut(s) 652
Bse21I CCTNAGG 2 cut(s) 12, 136
BseBI CCWGG 2 cut(s) 23, 219
BseGI GGATG 2 cut(s) 571, 622
BseLI CCNNNNNNNGG 1 cut(s) 150
BseMII CTCAG 3 cut(s) 26, 150, 336
BseX3I CGGCCG 1 cut(s) 152
BseXI GCAGC 2 cut(s) 225, 458
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 3 cut(s) 141, 154, 652
BshNI GGYRCC 1 cut(s) 181
BsiEI CGRYCG 1 cut(s) 155
BsiSI CCGG 2 cut(s) 200, 653
BslFI GGGAC 1 cut(s) 522
BslI CCNNNNNNNGG 1 cut(s) 150
BsmFI GGGAC 1 cut(s) 522
BsnI GGCC 3 cut(s) 141, 154, 652
Bsp143I GATC 2 cut(s) 395, 610
BspACI CCGC 5 cut(s) 19, 120, 123, 163, 637
BspANI GGCC 3 cut(s) 141, 154, 652
BspCNI CTCAG 3 cut(s) 25, 149, 337
BspLI GGNNCC 4 cut(s) 130, 142, 183, 223
BspQI GCTCTTC 1 cut(s) 344
BspT107I GGYRCC 1 cut(s) 181
BsrFI RCCGGY 1 cut(s) 652
BssAI RCCGGY 1 cut(s) 652
BssMI GATC 2 cut(s) 395, 610
BssNI GRCGYC 2 cut(s) 39, 182
BssSI CACGAG 1 cut(s) 96
Bst2BI CACGAG 1 cut(s) 96
Bst2UI CCWGG 2 cut(s) 23, 219
Bst4CI ACNGT 2 cut(s) 110, 269
Bst6I CTCTTC 1 cut(s) 344
BstACI GRCGYC 2 cut(s) 39, 182
BstDEI CTNAG 4 cut(s) 12, 136, 345, 399
BstF5I GGATG 2 cut(s) 571, 622
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 1 cut(s) 184
BstKTI GATC 2 cut(s) 398, 613
BstMBI GATC 2 cut(s) 395, 610
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 6 cut(s) 47, 100, 126, 205, 214, 446
BstNI CCWGG 2 cut(s) 23, 219
BstSCI CCNGG 2 cut(s) 21, 217
BstSFI CTRYAG 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 225, 458
BstV2I GAAGAC 1 cut(s) 577
BstX2I RGATCY 1 cut(s) 395
BstYI RGATCY 1 cut(s) 395
BstZI CGGCCG 1 cut(s) 152
Bsu36I CCTNAGG 2 cut(s) 12, 136
BsuI GTATCC 1 cut(s) 198
BsuRI GGCC 3 cut(s) 141, 154, 652
BtgZI GCGATG 2 cut(s) 31, 275
BtsCI GGATG 2 cut(s) 571, 622
BtsIMutI CAGTG 1 cut(s) 274
CfoI GCGC 1 cut(s) 184
Cfr10I RCCGGY 1 cut(s) 652
Cfr13I GGNCC 4 cut(s) 16, 25, 140, 221
CseI GACGC 1 cut(s) 47
Csp6I GTAC 1 cut(s) 600
CviAII CATG 1 cut(s) 562
CviQI GTAC 1 cut(s) 600
DdeI CTNAG 4 cut(s) 12, 136, 345, 399
DinI GGCGCC 1 cut(s) 183
DpnI GATC 2 cut(s) 397, 612
DpnII GATC 2 cut(s) 395, 610
EaeI YGGCCR 1 cut(s) 152
EagI CGGCCG 1 cut(s) 152
Eam1104I CTCTTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 344
EciI GGCGGA 1 cut(s) 138
EclXI CGGCCG 1 cut(s) 152
Eco47I GGWCC 3 cut(s) 16, 25, 221
Eco52I CGGCCG 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 432
Eco81I CCTNAGG 2 cut(s) 12, 136
EcoO109I RGGNCCY 1 cut(s) 140
EcoRII CCWGG 2 cut(s) 21, 217
EgeI GGCGCC 1 cut(s) 183
EheI GGCGCC 1 cut(s) 183
FaeI CATG 1 cut(s) 565
FaiI YATR 9 cut(s) 105, 291, 293, 309, 329, 381, 423, 563, 597
FaqI GGGAC 1 cut(s) 522
FatI CATG 1 cut(s) 561
Fnu4HI GCNGC 3 cut(s) 121, 239, 447
FokI GGATG 2 cut(s) 558, 629
Fsp4HI GCNGC 3 cut(s) 121, 239, 447
FspBI CTAG 1 cut(s) 443
GlaI GCGC 1 cut(s) 183
GluI GCNGC 3 cut(s) 121, 239, 447
HaeII RGCGCY 1 cut(s) 185
HaeIII GGCC 3 cut(s) 141, 154, 652
HapII CCGG 2 cut(s) 200, 653
HgaI GACGC 1 cut(s) 47
HhaI GCGC 1 cut(s) 184
Hin1I GRCGYC 2 cut(s) 39, 182
Hin1II CATG 1 cut(s) 565
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HindIII AAGCTT 2 cut(s) 284, 500
HinfI GANTC 2 cut(s) 53, 510
HpaII CCGG 2 cut(s) 200, 653
HphI GGTGA 2 cut(s) 80, 365
Hpy166II GTNNAC 1 cut(s) 658
Hpy188I TCNGA 2 cut(s) 346, 436
Hpy188III TCNNGA 7 cut(s) 14, 35, 323, 454, 476, 589, 614
Hpy8I GTNNAC 1 cut(s) 658
Hpy99I CGWCG 2 cut(s) 41, 153
HpyAV CCTTC 2 cut(s) 16, 222
HpyCH4III ACNGT 2 cut(s) 110, 269
HpyCH4V TGCA 2 cut(s) 208, 565
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 100, 126, 205, 214, 446
HpyF3I CTNAG 4 cut(s) 12, 136, 345, 399
Hsp92I GRCGYC 2 cut(s) 39, 182
Hsp92II CATG 1 cut(s) 565
HspAI GCGC 1 cut(s) 182
KasI GGCGCC 1 cut(s) 181
Kzo9I GATC 2 cut(s) 395, 610
LguI GCTCTTC 1 cut(s) 344
LmnI GCTCC 1 cut(s) 134
Lsp1109I GCAGC 2 cut(s) 225, 458
LweI GCATC 3 cut(s) 427, 481, 607
MaeI CTAG 1 cut(s) 443
MaeIII GTNAC 2 cut(s) 71, 313
MalI GATC 2 cut(s) 397, 612
MboI GATC 2 cut(s) 395, 610
MboII GAAGA 3 cut(s) 331, 405, 577
MflI RGATCY 1 cut(s) 395
MluCI AATT 3 cut(s) 356, 495, 556
Mly113I GGCGCC 1 cut(s) 182
MlyI GAGTC 1 cut(s) 504
MmeI TCCRAC 1 cut(s) 599
MseI TTAA 1 cut(s) 498
MslI CAYNNNNRTG 1 cut(s) 477
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 2 cut(s) 200, 653
MspR9I CCNGG 2 cut(s) 23, 219
MvaI CCWGG 2 cut(s) 23, 219
MwoI GCNNNNNNNGC 6 cut(s) 47, 100, 126, 205, 214, 446
NarI GGCGCC 1 cut(s) 182
NdeII GATC 2 cut(s) 395, 610
NlaIII CATG 1 cut(s) 565
NlaIV GGNNCC 4 cut(s) 130, 142, 183, 223
NmuCI GTSAC 1 cut(s) 71
PciSI GCTCTTC 1 cut(s) 344
PfeI GAWTC 1 cut(s) 53
PkrI GCNGC 3 cut(s) 122, 240, 448
PleI GAGTC 1 cut(s) 504
PluTI GGCGCC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 504
Psp6I CCWGG 2 cut(s) 21, 217
PspGI CCWGG 2 cut(s) 21, 217
PspN4I GGNNCC 4 cut(s) 130, 142, 183, 223
PspPI GGNCC 4 cut(s) 16, 25, 140, 221
PsuI RGATCY 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 1 cut(s) 601
RsaNI GTAC 1 cut(s) 600
RseI CAYNNNNRTG 1 cut(s) 477
SapI GCTCTTC 1 cut(s) 344
SaqAI TTAA 1 cut(s) 498
SatI GCNGC 3 cut(s) 121, 239, 447
Sau3AI GATC 2 cut(s) 395, 610
Sau96I GGNCC 4 cut(s) 16, 25, 140, 221
ScaI AGTACT 1 cut(s) 601
SchI GAGTC 1 cut(s) 504
ScrFI CCNGG 2 cut(s) 23, 219
SetI ASST 8 cut(s) 30, 137, 240, 288, 351, 412, 504, 596
SfaNI GCATC 3 cut(s) 427, 481, 607
SfcI CTRYAG 1 cut(s) 172
SfoI GGCGCC 1 cut(s) 183
SinI GGWCC 3 cut(s) 16, 25, 221
SmiMI CAYNNNNRTG 1 cut(s) 477
Sse9I AATT 3 cut(s) 356, 495, 556
SsiI CCGC 5 cut(s) 19, 120, 123, 163, 637
SspDI GGCGCC 1 cut(s) 181
SspMI CTAG 1 cut(s) 443
StyD4I CCNGG 2 cut(s) 21, 217
TaaI ACNGT 2 cut(s) 110, 269
TaqI TCGA 3 cut(s) 36, 322, 453
TasI AATT 3 cut(s) 356, 495, 556
TatI WGTACW 1 cut(s) 599
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 1 cut(s) 53
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 2 cut(s) 238, 446
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 3 cut(s) 173, 278, 550
TspRI CASTG 1 cut(s) 274
VpaK11BI GGWCC 3 cut(s) 16, 25, 221
XapI RAATTY 1 cut(s) 556
XspI CTAG 1 cut(s) 443
ZrmI AGTACT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.