Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002471

Physical Location & Seq

Genomic Coordinates Reverse (-)

1611015 .. 1614922

Gene Structure 3908 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1022200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1803 bp

ATGAGAAAACCCAAATCAAAGAAATCTCGCATGCAAAGTAAACACTCGGAGCTCCAGGAAGTCGAGCTTCTGAAATCGTGGATCGAATCCCAGCAACCCGAACGTGGTTCCAACCCCACATCTCTCCCCGCTTTGCCCTTGGACGCCCGCGCTGGCCGCCTCGACTCCACAAGATTTTCGCGCTACGCCGGCGCCACCAGGTTCGACCAGCTGCCCATCTCTAAGAAGACCAAGGACGCCTTACGCCAATCCAAGAAGTATGTCCAAATGAGCGATATTCAGAGGGCGTGTTTGCCCCACGCCTTATGCGGCAGAGATGTTCGCGGTGCTGCCAAGACTGGCTCCGGAAAGACTCTTGCTTTTGTTATTCCAATTGTGGAAAAATTGTACAGAGAGAGATGGTGTCCTCAGGATGGGGTGGGCAGCATCGTAATCTCTCCAACAAGGGAAATAGCCAGTCAAACATTTGATGTGTTGAGACTGGTGGGGAGGCACCATAGTTTTAGTGCTGCCCTCCTAATTGGAGGTCGCCGAGGAAAACATGTTGAAGCAGAGAAGGCACTCGTAAATGAGTGCAACATAGTGGTTTGCACGCCCGGCCGGCTTCTTCAGCACATGGATGAAACGCCGCATTTTGACTGTTCACAGCTTCAGGTCGACTTTATCGTCTCTATCAGTAATATTGTATTGAATCTGCAGTTTTTGTCCTTGACGAGGTGGATAGTATGCTCAGCGATCCAAGTTTTAAGCAGAATGAATGCAATCATTTCACAGCTACCGAAGCAAAGGCAAACCTTACTCTTCTCGGCAACTCAACCGAAGGATGTTAAGGATCTCGCAGCTCTATGTTTGAAGGATCCAGAATTTCTCAGCGTGCATGAGAAGTCTGTGACAGCCACTCCTAGTAATTTGCAGCAAACATTTGTCATCGTTCATCTTCACCAAAAGTTAGAGATGTTGTGGAGGTTCATAAAAAAAAATCTAAAATCTAGGGTTCTAGTGTTTCTATCAACCAGAAAGCAGGTACAATTTATATATGAAGCATTTACAACGCTTCGTCCTGGAATATCCTTGATGGCTTTATATGGTAAGTTGGATCAACTTAAAAGAATGGCTACGGCCTCAGAGTTTAGTAAACAACCTTCAGTACTTTTTTCGACTGATGTGGCTTCAAGAGGCATTGACTTCAACCAGGGAGTCGACTGGGTTGTTCAGGTGGATTGTCCAGGAGATGTTGCATCTTACATACACAGAGTTGGTCGAACCGCACGCTATGACTCGGTAGGGAAATCACTTTTATTTCTGATGCCGTCGGAAGAAAAAATGGTTGAGAAATTACAAGCGGCAAAAATACCTATACGGGAAATAAAGCCTAACAAGAACGTAGTGCAATCAGTGTCTGGAAAGTTGGCAGATTCTATAGTCCAAGACCCAGATCTACACGGTCTGGCTCAAAATGCCTTTAAAACGTATCTGAAGTCTGTTGACAAGAAGGACAAAGAGATTTTCGACGTTACAAAACTACCAATTGATGAGTTTTCTGCATCGATAGGTCTTGCATCGACCCCCAGAATCCGCTTTTTAAACAACAAAATCAAGTCCGCATCCAAGAAAGTATCAGAACTATCTCCCACTGTTGAACCAGAGCATTTGGACAAGGACAATGAATTGGAGCTGCTTCGTAAAGAGGAGCTTGATCTTGGTTATTCTAGTGACGAGGAAGGGGATAAAGATCCTCTTCTAACAAATGATATAGCACATGCCGCAGAAGGGGAAGGAAGAAAAATTGAACATATTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

601

Amino Acids

67.34

Weight (kDa)

9.31

Isoelectric Point (pI)

40.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	92 - 279	1.5e-28	DEAD/DEAH box helicase
Helicase_C	PF00271	315 - 425	7e-22	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	477 - 525	1.4e-11	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002471)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54910
fragaria_vesca	FvH4_4g36230 FvH4_4g36230 FvH4_4g36230 FvH4_5g34980
malus_domestica	MD08G1022200.v1.1 MD13G1010800.v1.1 MD15G1026900.v1.1
prunus_persica	Prupe.1G342200_v2.0.a1
pyrus_communis	pycom08g13590 pycom13g00910 pycom15g02270 pycom15g02280 pycom15g02290
rosa_chinensis	RchiOBHm_Chr1g0336991 RchiOBHm_Chr4g0445981
rosa_laevigata	RLG00000005702 RLG00000029316
rosa_multiflora	Rmu_co8191210.1_g000001 Rmu_sc0000115.1_g000004 Rmu_sc0003001.1_g000004 Rmu_sc0008543.1_g000002 Rmu_sc0009742.1_g000002 Rmu_sc0036469.1_g000003
rosa_roxburghii	Rroxscaffold_4G00315110 Rroxscaffold_5G00386390
rosa_rugosa	Rorug01G0129500 Rorug01G0130100 Rorug04G0366700 Rorug04G0366700
rosa_samantha	Rh1AG150500 Rh1BG118700 Rh1BG118900 Rh1CG141800 Rh1DG157800 Rh4AG428100 Rh4BG437700 Rh4CG454400 Rh4DG436200
rosa_wichuraiana	Rw1G012290 Rw4G036610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	665
Acc36I	ACCTGC	1 cut(s)	1012
AccB1I	GGYRCC	2 cut(s)	191, 492
AccI	GTMKAC	2 cut(s)	657, 1200
AccII	CGCG	3 cut(s)	150, 181, 324
AccIII	TCCGGA	1 cut(s)	344
AclWI	GGATC	7 cut(s)	89, 730, 840, 851, 864, 1104, 1727
AcoI	YGGCCR	2 cut(s)	154, 598
AcsI	RAATTY	1 cut(s)	863
AcuI	CTGAAG	4 cut(s)	593, 635, 1128, 1496
AcyI	GRCGYC	3 cut(s)	144, 192, 237
AfaI	GTAC	3 cut(s)	389, 1026, 1149
AfiI	CCNNNNNNNGG	4 cut(s)	104, 413, 714, 1770
AflIII	ACRYGT	1 cut(s)	541
AgsI	TTSAA	7 cut(s)	548, 691, 853, 1173, 1189, 1640, 1790
AjnI	CCWGG	5 cut(s)	54, 197, 1060, 1191, 1225
AluBI	AGCT	8 cut(s)	52, 67, 211, 649, 775, 842, 1675, 1693
AluI	AGCT	8 cut(s)	52, 67, 211, 649, 775, 842, 1675, 1693
Alw21I	GWGCWC	1 cut(s)	54
Alw26I	GTCTC	2 cut(s)	472, 673
AlwI	GGATC	7 cut(s)	89, 730, 840, 851, 864, 1104, 1727
AlwNI	CAGNNNCTG	1 cut(s)	1400
Aor13HI	TCCGGA	1 cut(s)	344
AoxI	GGCC	3 cut(s)	154, 598, 1119
ApeKI	GCWGC	7 cut(s)	211, 329, 423, 509, 839, 913, 1675
ApoI	RAATTY	1 cut(s)	863
AspLEI	GCGC	3 cut(s)	152, 183, 194
AsuC2I	CCSGG	1 cut(s)	597
AsuHPI	GGTGA	1 cut(s)	932
AxyI	CCTNAGG	1 cut(s)	408
BamHI	GGATCC	1 cut(s)	856
BanI	GGYRCC	2 cut(s)	191, 492
BanII	GRGCYC	1 cut(s)	54
BarI	GAAGNNNNNNTAC	2 cut(s)	1126, 1158
BbsI	GAAGAC	1 cut(s)	233
Bbv12I	GWGCWC	1 cut(s)	54
BbvI	GCAGC	7 cut(s)	198, 316, 435, 496, 851, 925, 1662
BccI	CCATC	4 cut(s)	224, 393, 407, 1069
BceAI	ACGGC	2 cut(s)	1134, 1294
BciT130I	CCWGG	5 cut(s)	56, 199, 1062, 1193, 1227
BcnI	CCSGG	1 cut(s)	597
BcoDI	GTCTC	2 cut(s)	472, 673
BfaI	CTAG	4 cut(s)	903, 990, 998, 1710
BfmI	CTRYAG	2 cut(s)	695, 1419
BfoI	RGCGCY	1 cut(s)	195
BfuAI	ACCTGC	1 cut(s)	1012
BglI	GCCNNNNNGGC	1 cut(s)	601
BglII	AGATCT	1 cut(s)	1435
BlpI	GCTNAGC	1 cut(s)	730
BmcAI	AGTACT	1 cut(s)	1149
Bme1390I	CCNGG	6 cut(s)	56, 199, 597, 1062, 1193, 1227
BmiI	GGNNCC	5 cut(s)	109, 193, 343, 494, 858
BmrFI	CCNGG	6 cut(s)	56, 199, 597, 1062, 1193, 1227
BmrI	ACTGGG	1 cut(s)	1213
BmsI	GCATC	6 cut(s)	435, 1247, 1296, 1553, 1568, 1613
BmuI	ACTGGG	1 cut(s)	1213
BpiI	GAAGAC	1 cut(s)	233
BpmI	CTGGAG	1 cut(s)	38
Bpu1102I	GCTNAGC	1 cut(s)	730
BpuMI	CCSGG	1 cut(s)	597
Bsa29I	ATCGAT	1 cut(s)	1547
BsaBI	GATNNNNATC	1 cut(s)	1731
BsaHI	GRCGYC	3 cut(s)	144, 192, 237
BsaJI	CCNNGG	4 cut(s)	138, 231, 532, 1192
BsaWI	WCCGGW	1 cut(s)	344
BsaXI	ACNNNNNCTCC	2 cut(s)	883, 913
Bsc4I	CCNNNNNNNGG	4 cut(s)	104, 413, 714, 1770
Bse118I	RCCGGY	2 cut(s)	188, 600
Bse1I	ACTGG	4 cut(s)	343, 456, 486, 1208
Bse21I	CCTNAGG	1 cut(s)	408
Bse8I	GATNNNNATC	1 cut(s)	1731
BseAI	TCCGGA	1 cut(s)	344
BseBI	CCWGG	5 cut(s)	56, 199, 1062, 1193, 1227
BseCI	ATCGAT	1 cut(s)	1547
BseDI	CCNNGG	4 cut(s)	138, 231, 532, 1192
BseGI	GGATG	4 cut(s)	418, 625, 829, 1604
BseJI	GATNNNNATC	1 cut(s)	1731
BseLI	CCNNNNNNNGG	4 cut(s)	104, 413, 714, 1770
BseMII	CTCAG	4 cut(s)	422, 744, 883, 1137
BseNI	ACTGG	4 cut(s)	343, 456, 486, 1208
BseRI	GAGGAG	1 cut(s)	1703
BseX3I	CGGCCG	1 cut(s)	598
BseXI	GCAGC	7 cut(s)	198, 316, 435, 496, 851, 925, 1662
BseYI	CCCAGC	1 cut(s)	90
Bsh1236I	CGCG	3 cut(s)	150, 181, 324
Bsh1285I	CGRYCG	1 cut(s)	601
BshFI	GGCC	3 cut(s)	156, 600, 1121
BshNI	GGYRCC	2 cut(s)	191, 492
BshVI	ATCGAT	1 cut(s)	1547
BsiEI	CGRYCG	1 cut(s)	601
BsiHKAI	GWGCWC	1 cut(s)	54
BsiSI	CCGG	4 cut(s)	189, 345, 597, 601
BslI	CCNNNNNNNGG	4 cut(s)	104, 413, 714, 1770
BsmAI	GTCTC	2 cut(s)	472, 673
BsmBI	CGTCTC	1 cut(s)	673
BsmI	GAATGC	1 cut(s)	763
BsnI	GGCC	3 cut(s)	156, 600, 1121
Bsp1286I	GDGCHC	1 cut(s)	54
Bsp13I	TCCGGA	1 cut(s)	344
Bsp1407I	TGTACA	1 cut(s)	387
Bsp143I	GATC	8 cut(s)	81, 735, 832, 856, 1096, 1435, 1696, 1732
Bsp1720I	GCTNAGC	1 cut(s)	730
BspANI	GGCC	3 cut(s)	156, 600, 1121
BspCNI	CTCAG	4 cut(s)	421, 743, 882, 1136
BspDI	ATCGAT	1 cut(s)	1547
BspEI	TCCGGA	1 cut(s)	344
BspFNI	CGCG	3 cut(s)	150, 181, 324
BspLI	GGNNCC	5 cut(s)	109, 193, 343, 494, 858
BspMAI	CTGCAG	1 cut(s)	699
BspMI	ACCTGC	1 cut(s)	1012
BspPI	GGATC	7 cut(s)	89, 730, 840, 851, 864, 1104, 1727
BspT107I	GGYRCC	2 cut(s)	191, 492
BsrFI	RCCGGY	2 cut(s)	188, 600
BsrGI	TGTACA	1 cut(s)	387
BsrI	ACTGG	4 cut(s)	343, 456, 486, 1208
BssAI	RCCGGY	2 cut(s)	188, 600
BssECI	CCNNGG	4 cut(s)	138, 231, 532, 1192
BssMI	GATC	8 cut(s)	81, 735, 832, 856, 1096, 1435, 1696, 1732
BssNI	GRCGYC	3 cut(s)	144, 192, 237
BssT1I	CCWWGG	2 cut(s)	138, 231
Bst2UI	CCWGG	5 cut(s)	56, 199, 1062, 1193, 1227
Bst4CI	ACNGT	3 cut(s)	641, 1445, 1636
Bst6I	CTCTTC	2 cut(s)	806, 1743
BstACI	GRCGYC	3 cut(s)	144, 192, 237
BstAUI	TGTACA	1 cut(s)	387
BstC8I	GCNNGC	8 cut(s)	32, 148, 154, 190, 593, 602, 875, 1270
BstDEI	CTNAG	5 cut(s)	222, 408, 730, 869, 1123
BstENI	CCTNNNNNAGG	1 cut(s)	712
BstF5I	GGATG	4 cut(s)	418, 625, 829, 1604
BstFNI	CGCG	3 cut(s)	150, 181, 324
BstH2I	RGCGCY	1 cut(s)	195
BstHHI	GCGC	3 cut(s)	152, 183, 194
BstKTI	GATC	8 cut(s)	84, 738, 835, 859, 1099, 1438, 1699, 1735
BstMAI	GTCTC	2 cut(s)	472, 673
BstMBI	GATC	8 cut(s)	81, 735, 832, 856, 1096, 1435, 1696, 1732
BstMCI	CGRYCG	1 cut(s)	601
BstMWI	GCNNNNNNNGC	9 cut(s)	156, 189, 557, 597, 601, 610, 781, 1457, 1763
BstNI	CCWGG	5 cut(s)	56, 199, 1062, 1193, 1227
BstNSI	RCATGY	3 cut(s)	34, 545, 1763
BstSCI	CCNGG	6 cut(s)	54, 197, 595, 1060, 1191, 1225
BstSFI	CTRYAG	2 cut(s)	695, 1419
BstUI	CGCG	3 cut(s)	150, 181, 324
BstV1I	GCAGC	7 cut(s)	198, 316, 435, 496, 851, 925, 1662
BstV2I	GAAGAC	1 cut(s)	233
BstX2I	RGATCY	4 cut(s)	832, 856, 1435, 1732
BstYI	RGATCY	4 cut(s)	832, 856, 1435, 1732
BstZI	CGGCCG	1 cut(s)	598
Bsu15I	ATCGAT	1 cut(s)	1547
Bsu36I	CCTNAGG	1 cut(s)	408
BsuRI	GGCC	3 cut(s)	156, 600, 1121
BsuTUI	ATCGAT	1 cut(s)	1547
BtsCI	GGATG	4 cut(s)	418, 625, 829, 1604
BtsIMutI	CAGTG	2 cut(s)	1401, 1632
BveI	ACCTGC	1 cut(s)	1012
Cac8I	GCNNGC	8 cut(s)	32, 148, 154, 190, 593, 602, 875, 1270
CaiI	CAGNNNCTG	1 cut(s)	1400
CfoI	GCGC	3 cut(s)	152, 183, 194
Cfr10I	RCCGGY	2 cut(s)	188, 600
ClaI	ATCGAT	1 cut(s)	1547
CseI	GACGC	2 cut(s)	152, 245
CsiI	ACCWGGT	1 cut(s)	197
Csp6I	GTAC	3 cut(s)	388, 1025, 1148
CviAII	CATG	5 cut(s)	31, 542, 616, 878, 1760
CviQI	GTAC	3 cut(s)	388, 1025, 1148
DdeI	CTNAG	5 cut(s)	222, 408, 730, 869, 1123
DinI	GGCGCC	1 cut(s)	193
DpnI	GATC	8 cut(s)	83, 737, 834, 858, 1098, 1437, 1698, 1734
DpnII	GATC	8 cut(s)	81, 735, 832, 856, 1096, 1435, 1696, 1732
DraI	TTTAAA	2 cut(s)	1465, 1584
DrdI	GACNNNNNNGTC	1 cut(s)	665
DseDI	GACNNNNNNGTC	1 cut(s)	665
EaeI	YGGCCR	2 cut(s)	154, 598
EagI	CGGCCG	1 cut(s)	598
Eam1104I	CTCTTC	2 cut(s)	806, 1743
EarI	CTCTTC	2 cut(s)	806, 1743
Ecl136II	GAGCTC	1 cut(s)	52
EclXI	CGGCCG	1 cut(s)	598
Eco130I	CCWWGG	2 cut(s)	138, 231
Eco24I	GRGCYC	1 cut(s)	54
Eco52I	CGGCCG	1 cut(s)	598
Eco53kI	GAGCTC	1 cut(s)	52
Eco57I	CTGAAG	4 cut(s)	593, 635, 1128, 1496
Eco81I	CCTNAGG	1 cut(s)	408
EcoICRI	GAGCTC	1 cut(s)	52
EcoNI	CCTNNNNNAGG	1 cut(s)	712
EcoRII	CCWGG	5 cut(s)	54, 197, 1060, 1191, 1225
EcoT14I	CCWWGG	2 cut(s)	138, 231
EcoT38I	GRGCYC	1 cut(s)	54
EgeI	GGCGCC	1 cut(s)	193
EheI	GGCGCC	1 cut(s)	193
ErhI	CCWWGG	2 cut(s)	138, 231
Esp3I	CGTCTC	1 cut(s)	673
FaeI	CATG	5 cut(s)	34, 545, 619, 881, 1763
FalI	AAGNNNNNCTT	8 cut(s)	51, 83, 224, 256, 1677, 1709, 1722, 1754
FatI	CATG	5 cut(s)	30, 541, 615, 877, 1759
FauI	CCCGC	2 cut(s)	136, 155
FblI	GTMKAC	2 cut(s)	657, 1200
FokI	GGATG	4 cut(s)	425, 632, 836, 1591
FriOI	GRGCYC	1 cut(s)	54
FspBI	CTAG	4 cut(s)	903, 990, 998, 1710
GlaI	GCGC	3 cut(s)	151, 182, 193
GsaI	CCCAGC	1 cut(s)	94
GsuI	CTGGAG	1 cut(s)	38
HaeII	RGCGCY	1 cut(s)	195
HaeIII	GGCC	3 cut(s)	156, 600, 1121
HapII	CCGG	4 cut(s)	189, 345, 597, 601
HgaI	GACGC	2 cut(s)	152, 245
HhaI	GCGC	3 cut(s)	152, 183, 194
Hin1I	GRCGYC	3 cut(s)	144, 192, 237
Hin1II	CATG	5 cut(s)	34, 545, 619, 881, 1763
Hin6I	GCGC	3 cut(s)	150, 181, 192
HinP1I	GCGC	3 cut(s)	150, 181, 192
HincII	GTYRAC	3 cut(s)	658, 1201, 1486
HindII	GTYRAC	3 cut(s)	658, 1201, 1486
HinfI	GANTC	8 cut(s)	86, 164, 352, 691, 1197, 1277, 1415, 1572
HpaII	CCGG	4 cut(s)	189, 345, 597, 601
HphI	GGTGA	1 cut(s)	932
Hpy166II	GTNNAC	6 cut(s)	41, 644, 658, 1136, 1201, 1486
Hpy188I	TCNGA	8 cut(s)	49, 72, 282, 1126, 1305, 1315, 1476, 1621
Hpy188III	TCNNGA	5 cut(s)	345, 410, 860, 1173, 1401
Hpy8I	GTNNAC	6 cut(s)	41, 644, 658, 1136, 1201, 1486
Hpy99I	CGWCG	2 cut(s)	1315, 1514
HpyAV	CCTTC	8 cut(s)	550, 814, 847, 1152, 1486, 1715, 1763, 1769
HpyCH4III	ACNGT	3 cut(s)	641, 1445, 1636
HpyCH4IV	ACGT	4 cut(s)	103, 1383, 1469, 1512
HpyF10VI	GCNNNNNNNGC	9 cut(s)	156, 189, 557, 597, 601, 610, 781, 1457, 1763
HpyF3I	CTNAG	5 cut(s)	222, 408, 730, 869, 1123
HpySE526I	ACGT	4 cut(s)	103, 1383, 1469, 1512
Hsp92I	GRCGYC	3 cut(s)	144, 192, 237
Hsp92II	CATG	5 cut(s)	34, 545, 619, 881, 1763
HspAI	GCGC	3 cut(s)	150, 181, 192
KasI	GGCGCC	1 cut(s)	191
Kpn2I	TCCGGA	1 cut(s)	344
KroI	GCCGGC	2 cut(s)	188, 600
KroNI	GCCGGC	2 cut(s)	190, 602
Kzo9I	GATC	8 cut(s)	81, 735, 832, 856, 1096, 1435, 1696, 1732
LmnI	GCTCC	5 cut(s)	49, 57, 347, 1672, 1690
Lsp1109I	GCAGC	7 cut(s)	198, 316, 435, 496, 851, 925, 1662
LweI	GCATC	6 cut(s)	435, 1247, 1296, 1553, 1568, 1613
MabI	ACCWGGT	1 cut(s)	197
MaeI	CTAG	4 cut(s)	903, 990, 998, 1710
MaeII	ACGT	4 cut(s)	103, 1383, 1469, 1512
MaeIII	GTNAC	3 cut(s)	889, 1513, 1712
MalI	GATC	8 cut(s)	83, 737, 834, 858, 1098, 1437, 1698, 1734
MboI	GATC	8 cut(s)	81, 735, 832, 856, 1096, 1435, 1696, 1732
MboII	GAAGA	7 cut(s)	238, 599, 793, 929, 1328, 1730, 1791
MfeI	CAATTG	2 cut(s)	372, 1527
MflI	RGATCY	4 cut(s)	832, 856, 1435, 1732
MhlI	GDGCHC	1 cut(s)	54
Mly113I	GGCGCC	1 cut(s)	192
MlyI	GAGTC	4 cut(s)	158, 346, 1206, 1271
MmeI	TCCRAC	4 cut(s)	135, 464, 1074, 1293
MreI	CGCCGGCG	1 cut(s)	188
MroI	TCCGGA	1 cut(s)	344
MroNI	GCCGGC	2 cut(s)	188, 600
MseI	TTAA	5 cut(s)	746, 828, 1104, 1464, 1583
MslI	CAYNNNNRTG	1 cut(s)	618
MspA1I	CMGCKG	1 cut(s)	211
MspI	CCGG	4 cut(s)	189, 345, 597, 601
MspR9I	CCNGG	6 cut(s)	56, 199, 597, 1062, 1193, 1227
MunI	CAATTG	2 cut(s)	372, 1527
Mva1269I	GAATGC	1 cut(s)	763
MvaI	CCWGG	5 cut(s)	56, 199, 1062, 1193, 1227
MvnI	CGCG	3 cut(s)	150, 181, 324
MwoI	GCNNNNNNNGC	9 cut(s)	156, 189, 557, 597, 601, 610, 781, 1457, 1763
NaeI	GCCGGC	2 cut(s)	190, 602
NarI	GGCGCC	1 cut(s)	192
NciI	CCSGG	1 cut(s)	597
NdeII	GATC	8 cut(s)	81, 735, 832, 856, 1096, 1435, 1696, 1732
NgoMIV	GCCGGC	2 cut(s)	188, 600
NlaIII	CATG	5 cut(s)	34, 545, 619, 881, 1763
NlaIV	GGNNCC	5 cut(s)	109, 193, 343, 494, 858
NmeAIII	GCCGAG	2 cut(s)	557, 785
NmuCI	GTSAC	2 cut(s)	889, 1712
NspI	RCATGY	3 cut(s)	34, 545, 1763
PaeI	GCATGC	1 cut(s)	34
PciI	ACATGT	1 cut(s)	541
PcsI	WCGNNNNNNNCGW	1 cut(s)	663
PctI	GAATGC	1 cut(s)	763
PdiI	GCCGGC	2 cut(s)	190, 602
PfeI	GAWTC	4 cut(s)	86, 691, 1415, 1572
PfoI	TCCNGGA	3 cut(s)	54, 1060, 1225
PleI	GAGTC	4 cut(s)	158, 346, 1205, 1271
PluTI	GGCGCC	1 cut(s)	195
PpsI	GAGTC	4 cut(s)	158, 346, 1205, 1271
PscI	ACATGT	1 cut(s)	541
Psp124BI	GAGCTC	1 cut(s)	54
Psp6I	CCWGG	5 cut(s)	54, 197, 1060, 1191, 1225
PspFI	CCCAGC	1 cut(s)	90
PspGI	CCWGG	5 cut(s)	54, 197, 1060, 1191, 1225
PspN4I	GGNNCC	5 cut(s)	109, 193, 343, 494, 858
PstI	CTGCAG	1 cut(s)	699
PstNI	CAGNNNCTG	1 cut(s)	1400
PsuI	RGATCY	4 cut(s)	832, 856, 1435, 1732
PvuII	CAGCTG	1 cut(s)	211
RsaI	GTAC	3 cut(s)	389, 1026, 1149
RsaNI	GTAC	3 cut(s)	388, 1025, 1148
RseI	CAYNNNNRTG	1 cut(s)	618
SacI	GAGCTC	1 cut(s)	54
SalI	GTCGAC	2 cut(s)	656, 1199
SaqAI	TTAA	5 cut(s)	746, 828, 1104, 1464, 1583
Sau3AI	GATC	8 cut(s)	81, 735, 832, 856, 1096, 1435, 1696, 1732
ScaI	AGTACT	1 cut(s)	1149
SchI	GAGTC	4 cut(s)	158, 346, 1206, 1271
ScrFI	CCNGG	6 cut(s)	56, 199, 597, 1062, 1193, 1227
SduI	GDGCHC	1 cut(s)	54
SexAI	ACCWGGT	1 cut(s)	197
SfaNI	GCATC	6 cut(s)	435, 1247, 1296, 1553, 1568, 1613
SfcI	CTRYAG	2 cut(s)	695, 1419
SfoI	GGCGCC	1 cut(s)	193
SgrAI	CRCCGGYG	1 cut(s)	188
SmiMI	CAYNNNNRTG	1 cut(s)	618
SphI	GCATGC	1 cut(s)	34
SspDI	GGCGCC	1 cut(s)	191
SspI	AATATT	1 cut(s)	682
SspMI	CTAG	4 cut(s)	903, 990, 998, 1710
SstI	GAGCTC	1 cut(s)	54
StyD4I	CCNGG	6 cut(s)	54, 197, 595, 1060, 1191, 1225
StyI	CCWWGG	2 cut(s)	138, 231
TaaI	ACNGT	3 cut(s)	641, 1445, 1636
TaiI	ACGT	4 cut(s)	106, 1386, 1472, 1515
TatI	WGTACW	2 cut(s)	387, 1147
TauI	GCSGC	5 cut(s)	159, 312, 631, 1346, 1766
TfiI	GAWTC	4 cut(s)	86, 691, 1415, 1572
Tru1I	TTAA	5 cut(s)	746, 828, 1104, 1464, 1583
Tru9I	TTAA	5 cut(s)	746, 828, 1104, 1464, 1583
TscAI	CASTG	2 cut(s)	1401, 1639
TseFI	GTSAC	2 cut(s)	889, 1712
TseI	GCWGC	7 cut(s)	211, 329, 423, 509, 839, 913, 1675
Tsp45I	GTSAC	2 cut(s)	889, 1712
TspDTI	ATGAA	6 cut(s)	636, 770, 923, 958, 1053, 1680
TspRI	CASTG	2 cut(s)	1401, 1639
XagI	CCTNNNNNAGG	1 cut(s)	712
XapI	RAATTY	1 cut(s)	863
XceI	RCATGY	3 cut(s)	34, 545, 1763
XmiI	GTMKAC	2 cut(s)	657, 1200
XspI	CTAG	4 cut(s)	903, 990, 998, 1710
ZrmI	AGTACT	1 cut(s)	1149

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.