Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0002471

Physical Location & Seq

Genomic Coordinates Forward (+)

12383515 .. 12387249

Gene Structure 3735 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g13590.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2364 bp

ATGGATGGAGACCTTAAGCATAAAATACAAGTTGACCTCTGCCGCTTCCCTCGCGCTCTGTCTTTTCATATTTCCACAGTCCGAGGTAGAAACACAACCAACGAAGCGACGATGAGAAAACTCAAATCAACGAAATCTCGCATCCAAAGTCAACACTCTGAGCTCCACGAAATCGAGCTTCTGAAATCGTGGATCGAATCCCAGCAACCCGAACGCGGCTCCAACCCCATGTCTCTCCCCGCTTTGCCCTCAGACGCCCGCGCTGGCCGCCTCGACTCCACACGATTTTCGCGCTACGCCGGTGCCACCAGGTTCGACCAGCTGCCCATTTCTAAGAAGACCAAGGACGCCTTGCGCCAATGCAACAAGTACGTCCAAATGAGCGATATTCAGAGGGCGTGTTTGCCCCACGCCTTATGCGGCAGAGATGTTCGCGGTGCTGCCAAGACTGGCTCCGGAAAGACTCTTGCTTTTGTTATTCCAATTGTGGAAAAATTGTACAGAGGGAGATGGTGTCCTCAGGATGGGGTGGGCAGCATCGTAATCTCTCCGACGAGGGAAATAGCCAGTCAAACATTTGACGTGTTGAGATTGGTGGGGAGGCACCATAGTTTTAGTGCTGCCCTCCTAATTGGTGGTCGCCGTGGAAAAGATGTCCAAGTAGAGAAGGAGCTCGTAAATGAGTGCAACATATTGGTTTGCACGCCCGGCCGGCTTCTTCAGCACATGGATGAAACACCGCATTTTGACTGTTCACAGCTTCAGGTTTTTGTCCTTGACGAGGTGGATAGTATGCTCAGCGATCCAAGTTTTAAGCAGACAATGAATGCAATCATTTCAGAGCTACCAAAGCAAAGGCAAACCTTGCTCTTCTCGGCAACTCACCCAAAGGATGCTAAGGATCTTGCGGTTCTATGTTTGAAGGATCCAGAATTTCTGAGCGTGCATGAGAAGTCTGTGACAGCCACTCCTAATAAATTGCGGCAAACATTTGTCATCGTCCCTCTTCACCAAAAGTTAGACATGTTGTGGAGCTTCATAAAAAGAAATCTAAAATCCAGGATTCTAGTGTTTCTATCGACCAGAAAGCAAGTACAATTTGTATGTGAAGCATTTAAAATGCTTTCTCCTACAATAACCTGGATGGCTCTTTATGGTAAGTTGGATCAACGTAGAAGAATGGCAACCGTCTCAGAGTTTAGTAACCAACCTTCCGTACTTTTTTCGACTGATGTGGCTTCAAGAGGCCTTGATTTCAACCAGGGAGTTGACTGGGTTGTTCAGGTGGATTGTCCAGGAGATGTTGCATCTTACATACACAGAGTTGGTCGAACTGCTCGCTTTGATTCAGTAGGGAAATCACTTTTATTTCTGATGCCGTCTGAAGAAAAAATGCTTGAGAAATTACAAGCGGCAAACATACCTATACGGGAAATAAAGCCTAACAAGAACGTAGGGCAATCAGTGTCTGGAAAGTTGGCAAATTTTATAGTCCAGTACCCAGGTCTACTGCATGCTCGGGCTCAAAGTGCCCTTAAAACGTATCTGAAGTCTGTTACCAAGAAGGACAAAGAGATTTTTGACGTTACGAAACTACCAATTGATGAGTTTTCTGCATCCATAGGTCTAGCATCCACCCCCAGAATACGTTTTTTAAGCAACAAAATCAAGTCCTCATCCAAGAAGGCGTCAGAACTATCTCCCGTTGTTGAACCAGAGCTTTTGGACAAGGACCATGAATTGGAGCTGCTTCCTAAAGAGGAGCTTGATCTTGGTGCTTCTAGTGACGAGGAAAGGGATAAAGATCCTCTTCCAACAAATGATACAGCGGATATTTCACCGCCTTGTACAGCGTCAACTCGGGTTTCGAAGAAGAAGAAGAAGTTAAAGATCAATATCCATAGACCAGTGGGAAGTAGAGTTGTTTTTGATGAGGAAGGCAACACGCTACCACCACTAGCAAGGCTGGCTGATATCAGCAATGCCAGTGGTCTGCTCGATCAAATTCAAGACAAGAAAAACGAATATTATAAGAAGCTTAGGGAAGGTTTGAAGAAGGTGGACAAGGATGACAAGGTTGTGGACGGTCATCGCCGCCGTGAGAAGCGTTTGAAGGATAAGATTAGGTCGAAGAGAGGGGACACAGACGAGGAGCCGGAGGAGGAGGAGGAGGAGGATGTTTCAGATTCGGAAGGAGAGCCGTGTGCCGATCGACCCCACAAAAGGCCAAAGATATACTTTGGTAGTGATAGCGACGGGGAGATAAAAGAAAGAAAGGACAAACCGGGGTTCGTTGCTGACTCAATATCTTTGGAGGAACAAGAGGCTGTTGCTCTCAAATTGTTAAGTGCCATGCACTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

788

Amino Acids

88.58

Weight (kDa)

9.05

Isoelectric Point (pI)

47.4

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002471)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54910
fragaria_vesca	FvH4_4g36230 FvH4_4g36230 FvH4_4g36230 FvH4_5g34980
malus_domestica	MD08G1022200.v1.1 MD13G1010800.v1.1 MD15G1026900.v1.1
prunus_persica	Prupe.1G342200_v2.0.a1
pyrus_communis	pycom08g13590 pycom13g00910 pycom15g02270 pycom15g02280 pycom15g02290
rosa_chinensis	RchiOBHm_Chr1g0336991 RchiOBHm_Chr4g0445981
rosa_laevigata	RLG00000005702 RLG00000029316
rosa_multiflora	Rmu_co8191210.1_g000001 Rmu_sc0000115.1_g000004 Rmu_sc0003001.1_g000004 Rmu_sc0008543.1_g000002 Rmu_sc0009742.1_g000002 Rmu_sc0036469.1_g000003
rosa_roxburghii	Rroxscaffold_4G00315110 Rroxscaffold_5G00386390
rosa_rugosa	Rorug01G0129500 Rorug01G0130100 Rorug04G0366700 Rorug04G0366700
rosa_samantha	Rh1AG150500 Rh1BG118700 Rh1BG118900 Rh1CG141800 Rh1DG157800 Rh4AG428100 Rh4BG437700 Rh4CG454400 Rh4DG436200
rosa_wichuraiana	Rw1G012290 Rw4G036610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2031
AccB1I	GGYRCC	2 cut(s)	302, 603
AccB7I	CCANNNNNTGG	1 cut(s)	1741
AccI	GTMKAC	1 cut(s)	1507
AccII	CGCG	5 cut(s)	54, 216, 261, 292, 435
AccIII	TCCGGA	1 cut(s)	455
AclWI	GGATC	7 cut(s)	200, 797, 909, 920, 933, 1173, 1799
AcoI	YGGCCR	2 cut(s)	265, 709
AcsI	RAATTY	3 cut(s)	932, 1483, 2004
AcuI	CTGAAG	4 cut(s)	704, 746, 1404, 1568
AcyI	GRCGYC	3 cut(s)	255, 348, 1688
AfaI	GTAC	6 cut(s)	371, 500, 1095, 1218, 1499, 1849
AfiI	CCNNNNNNNGG	5 cut(s)	215, 524, 781, 1741, 2223
AflII	CTTAAG	1 cut(s)	14
AflIII	ACRYGT	2 cut(s)	582, 1023
AgsI	TTSAA	7 cut(s)	922, 1242, 1258, 1712, 2009, 2053, 2113
AjiI	CACGTC	1 cut(s)	583
AjnI	CCWGG	6 cut(s)	308, 1058, 1139, 1260, 1294, 1501
Alw21I	GWGCWC	2 cut(s)	165, 675
Alw26I	GTCTC	3 cut(s)	3, 237, 1195
AlwI	GGATC	7 cut(s)	200, 797, 909, 920, 933, 1173, 1799
AlwNI	CAGNNNCTG	1 cut(s)	1469
Ama87I	CYCGRG	2 cut(s)	1518, 1860
Aor13HI	TCCGGA	1 cut(s)	455
AoxI	GGCC	4 cut(s)	265, 709, 1246, 2225
ApeKI	GCWGC	5 cut(s)	322, 440, 534, 620, 1747
ApoI	RAATTY	3 cut(s)	932, 1483, 2004
AspLEI	GCGC	4 cut(s)	56, 263, 294, 357
AspS9I	GGNCC	1 cut(s)	1732
AsuC2I	CCSGG	2 cut(s)	708, 2286
AsuHPI	GGTGA	3 cut(s)	875, 1001, 1830
AsuII	TTCGAA	1 cut(s)	1868
AvaI	CYCGRG	2 cut(s)	1518, 1860
AvaII	GGWCC	1 cut(s)	1732
AxyI	CCTNAGG	1 cut(s)	519
BaeGI	GKGCMC	1 cut(s)	1534
BamHI	GGATCC	1 cut(s)	925
BanI	GGYRCC	2 cut(s)	302, 603
BanII	GRGCYC	3 cut(s)	165, 675, 1525
BarI	GAAGNNNNNNTAC	2 cut(s)	1195, 1227
BbsI	GAAGAC	1 cut(s)	344
Bbv12I	GWGCWC	2 cut(s)	165, 675
BbvI	GCAGC	5 cut(s)	309, 427, 546, 607, 1734
BccI	CCATC	3 cut(s)	504, 518, 1138
BceAI	ACGGC	4 cut(s)	627, 1363, 2082, 2185
BciT130I	CCWGG	6 cut(s)	310, 1060, 1141, 1262, 1296, 1503
BcnI	CCSGG	2 cut(s)	708, 2286
BcoDI	GTCTC	3 cut(s)	3, 237, 1195
BfaI	CTAG	4 cut(s)	1067, 1628, 1782, 1958
BfrI	CTTAAG	1 cut(s)	14
BglI	GCCNNNNNGGC	1 cut(s)	712
BlpI	GCTNAGC	1 cut(s)	797
Bme1390I	CCNGG	8 cut(s)	310, 708, 1060, 1141, 1262, 1296, 1503, 2286
Bme18I	GGWCC	1 cut(s)	1732
BmeT110I	CYCGRG	2 cut(s)	1518, 1860
BmgBI	CACGTC	1 cut(s)	583
BmgT120I	GGNCC	1 cut(s)	1732
BmiI	GGNNCC	6 cut(s)	220, 304, 454, 605, 927, 2154
BmrFI	CCNGG	8 cut(s)	310, 708, 1060, 1141, 1262, 1296, 1503, 2286
BmrI	ACTGGG	1 cut(s)	1282
BmsI	GCATC	7 cut(s)	150, 546, 883, 1316, 1365, 1625, 1640
BmuI	ACTGGG	1 cut(s)	1282
BpiI	GAAGAC	1 cut(s)	344
Bpu10I	CCTNAGC	2 cut(s)	897, 2039
Bpu1102I	GCTNAGC	1 cut(s)	797
Bpu14I	TTCGAA	1 cut(s)	1868
BpuEI	CTTGAG	1 cut(s)	1418
BpuMI	CCSGG	2 cut(s)	708, 2286
BsaBI	GATNNNNATC	2 cut(s)	1803, 1895
BsaHI	GRCGYC	3 cut(s)	255, 348, 1688
BsaI	GGTCTC	1 cut(s)	3
BsaJI	CCNNGG	6 cut(s)	82, 342, 643, 1261, 1501, 2285
BsaWI	WCCGGW	1 cut(s)	455
BsaXI	ACNNNNNCTCC	4 cut(s)	499, 529, 952, 982
Bsc4I	CCNNNNNNNGG	5 cut(s)	215, 524, 781, 1741, 2223
Bse118I	RCCGGY	2 cut(s)	299, 711
Bse1I	ACTGG	6 cut(s)	454, 567, 1277, 1495, 1907, 1986
Bse21I	CCTNAGG	1 cut(s)	519
Bse3DI	GCAATG	1 cut(s)	1987
Bse8I	GATNNNNATC	2 cut(s)	1803, 1895
BseAI	TCCGGA	1 cut(s)	455
BseBI	CCWGG	6 cut(s)	310, 1060, 1141, 1262, 1296, 1503
BseDI	CCNNGG	6 cut(s)	82, 342, 643, 1261, 1501, 2285
BseJI	GATNNNNATC	2 cut(s)	1803, 1895
BseLI	CCNNNNNNNGG	5 cut(s)	215, 524, 781, 1741, 2223
BseMI	GCAATG	1 cut(s)	1987
BseMII	CTCAG	6 cut(s)	150, 264, 533, 811, 929, 1206
BseNI	ACTGG	6 cut(s)	454, 567, 1277, 1495, 1907, 1986
BseRI	GAGGAG	7 cut(s)	1775, 2165, 2174, 2177, 2180, 2183, 2186
BseSI	GKGCMC	1 cut(s)	1534
BseX3I	CGGCCG	1 cut(s)	709
BseXI	GCAGC	5 cut(s)	309, 427, 546, 607, 1734
BseYI	CCCAGC	1 cut(s)	201
Bsh1236I	CGCG	5 cut(s)	54, 216, 261, 292, 435
Bsh1285I	CGRYCG	2 cut(s)	712, 2212
BshFI	GGCC	4 cut(s)	267, 711, 1248, 2227
BshNI	GGYRCC	2 cut(s)	302, 603
BsiEI	CGRYCG	2 cut(s)	712, 2212
BsiHKAI	GWGCWC	2 cut(s)	165, 675
BsiHKCI	CYCGRG	2 cut(s)	1518, 1860
BsiSI	CCGG	6 cut(s)	300, 456, 708, 712, 2156, 2285
BslFI	GGGAC	2 cut(s)	986, 2153
BslI	CCNNNNNNNGG	5 cut(s)	215, 524, 781, 1741, 2223
BsmAI	GTCTC	3 cut(s)	3, 237, 1195
BsmBI	CGTCTC	1 cut(s)	1195
BsmFI	GGGAC	2 cut(s)	986, 2153
BsmI	GAATGC	1 cut(s)	832
BsnI	GGCC	4 cut(s)	267, 711, 1248, 2227
Bso31I	GGTCTC	1 cut(s)	3
BsoBI	CYCGRG	2 cut(s)	1518, 1860
Bsp119I	TTCGAA	1 cut(s)	1868
Bsp1286I	GDGCHC	4 cut(s)	165, 675, 1525, 1534
Bsp13I	TCCGGA	1 cut(s)	455
Bsp1407I	TGTACA	2 cut(s)	498, 1847
Bsp1720I	GCTNAGC	1 cut(s)	797
BspANI	GGCC	4 cut(s)	267, 711, 1248, 2227
BspCNI	CTCAG	6 cut(s)	151, 263, 532, 810, 930, 1205
BspEI	TCCGGA	1 cut(s)	455
BspFNI	CGCG	5 cut(s)	54, 216, 261, 292, 435
BspLI	GGNNCC	6 cut(s)	220, 304, 454, 605, 927, 2154
BspPI	GGATC	7 cut(s)	200, 797, 909, 920, 933, 1173, 1799
BspQI	GCTCTTC	1 cut(s)	875
BspT104I	TTCGAA	1 cut(s)	1868
BspT107I	GGYRCC	2 cut(s)	302, 603
BspTI	CTTAAG	1 cut(s)	14
BspTNI	GGTCTC	1 cut(s)	3
BsrDI	GCAATG	1 cut(s)	1987
BsrFI	RCCGGY	2 cut(s)	299, 711
BsrGI	TGTACA	2 cut(s)	498, 1847
BsrI	ACTGG	6 cut(s)	454, 567, 1277, 1495, 1907, 1986
BssAI	RCCGGY	2 cut(s)	299, 711
BssECI	CCNNGG	6 cut(s)	82, 342, 643, 1261, 1501, 2285
BssNI	GRCGYC	3 cut(s)	255, 348, 1688
BssT1I	CCWWGG	1 cut(s)	342
Bst2UI	CCWGG	6 cut(s)	310, 1060, 1141, 1262, 1296, 1503
Bst4CI	ACNGT	4 cut(s)	79, 752, 1189, 2087
Bst6I	CTCTTC	4 cut(s)	875, 1011, 1815, 2126
BstACI	GRCGYC	3 cut(s)	255, 348, 1688
BstAFI	CTTAAG	1 cut(s)	14
BstAPI	GCANNNNNTGC	1 cut(s)	865
BstAUI	TGTACA	2 cut(s)	498, 1847
BstBI	TTCGAA	1 cut(s)	1868
BstC8I	GCNNGC	8 cut(s)	259, 265, 704, 713, 944, 1339, 1515, 1968
BstDEI	CTNAG	9 cut(s)	159, 250, 333, 519, 797, 897, 938, 1192, 2039
BstDSI	CCRYGG	1 cut(s)	643
BstENI	CCTNNNNNAGG	1 cut(s)	779
BstFNI	CGCG	5 cut(s)	54, 216, 261, 292, 435
BstHHI	GCGC	4 cut(s)	56, 263, 294, 357
BstMAI	GTCTC	3 cut(s)	3, 237, 1195
BstMCI	CGRYCG	2 cut(s)	712, 2212
BstMWI	GCNNNNNNNGC	9 cut(s)	51, 267, 708, 712, 721, 850, 865, 1529, 1967
BstNI	CCWGG	6 cut(s)	310, 1060, 1141, 1262, 1296, 1503
BstNSI	RCATGY	2 cut(s)	1027, 1517
BstSCI	CCNGG	8 cut(s)	308, 706, 1058, 1139, 1260, 1294, 1501, 2284
BstSLI	GKGCMC	1 cut(s)	1534
BstUI	CGCG	5 cut(s)	54, 216, 261, 292, 435
BstV1I	GCAGC	5 cut(s)	309, 427, 546, 607, 1734
BstV2I	GAAGAC	1 cut(s)	344
BstX2I	RGATCY	3 cut(s)	901, 925, 1804
BstYI	RGATCY	3 cut(s)	901, 925, 1804
BstZI	CGGCCG	1 cut(s)	709
Bsu36I	CCTNAGG	1 cut(s)	519
BsuRI	GGCC	4 cut(s)	267, 711, 1248, 2227
BtgI	CCRYGG	1 cut(s)	643
BtgZI	GCGATG	1 cut(s)	2075
BtrI	CACGTC	1 cut(s)	583
BtsIMutI	CAGTG	3 cut(s)	1470, 1914, 1993
Cac8I	GCNNGC	8 cut(s)	259, 265, 704, 713, 944, 1339, 1515, 1968
CaiI	CAGNNNCTG	1 cut(s)	1469
CfoI	GCGC	4 cut(s)	56, 263, 294, 357
Cfr10I	RCCGGY	2 cut(s)	299, 711
Cfr13I	GGNCC	1 cut(s)	1732
CseI	GACGC	4 cut(s)	263, 356, 1677, 1842
CsiI	ACCWGGT	1 cut(s)	308
Csp6I	GTAC	6 cut(s)	370, 499, 1094, 1217, 1498, 1848
CspCI	CAANNNNNGTGG	2 cut(s)	1969, 2004
CviAII	CATG	7 cut(s)	229, 727, 947, 1024, 1514, 1736, 2353
CviQI	GTAC	6 cut(s)	370, 499, 1094, 1217, 1498, 1848
DdeI	CTNAG	9 cut(s)	159, 250, 333, 519, 797, 897, 938, 1192, 2039
DraI	TTTAAA	1 cut(s)	1117
EaeI	YGGCCR	2 cut(s)	265, 709
EagI	CGGCCG	1 cut(s)	709
Eam1104I	CTCTTC	4 cut(s)	875, 1011, 1815, 2126
EarI	CTCTTC	4 cut(s)	875, 1011, 1815, 2126
Ecl136II	GAGCTC	2 cut(s)	163, 673
EclXI	CGGCCG	1 cut(s)	709
Eco130I	CCWWGG	1 cut(s)	342
Eco147I	AGGCCT	1 cut(s)	1248
Eco24I	GRGCYC	3 cut(s)	165, 675, 1525
Eco31I	GGTCTC	1 cut(s)	3
Eco32I	GATATC	1 cut(s)	1975
Eco47I	GGWCC	1 cut(s)	1732
Eco52I	CGGCCG	1 cut(s)	709
Eco53kI	GAGCTC	2 cut(s)	163, 673
Eco57I	CTGAAG	4 cut(s)	704, 746, 1404, 1568
Eco81I	CCTNAGG	1 cut(s)	519
Eco88I	CYCGRG	2 cut(s)	1518, 1860
EcoICRI	GAGCTC	2 cut(s)	163, 673
EcoNI	CCTNNNNNAGG	1 cut(s)	779
EcoRII	CCWGG	6 cut(s)	308, 1058, 1139, 1260, 1294, 1501
EcoRV	GATATC	1 cut(s)	1975
EcoT14I	CCWWGG	1 cut(s)	342
EcoT38I	GRGCYC	3 cut(s)	165, 675, 1525
ErhI	CCWWGG	1 cut(s)	342
Esp3I	CGTCTC	1 cut(s)	1195
FaeI	CATG	7 cut(s)	232, 730, 950, 1027, 1517, 1739, 2356
FalI	AAGNNNNNCTT	8 cut(s)	335, 367, 1749, 1781, 1794, 1826, 2222, 2254
FaqI	GGGAC	2 cut(s)	986, 2153
FatI	CATG	7 cut(s)	228, 726, 946, 1023, 1513, 1735, 2352
FauI	CCCGC	2 cut(s)	247, 266
FblI	GTMKAC	1 cut(s)	1507
FriOI	GRGCYC	3 cut(s)	165, 675, 1525
FspBI	CTAG	4 cut(s)	1067, 1628, 1782, 1958
GlaI	GCGC	4 cut(s)	55, 262, 293, 356
GsaI	CCCAGC	1 cut(s)	205
HaeIII	GGCC	4 cut(s)	267, 711, 1248, 2227
HapII	CCGG	6 cut(s)	300, 456, 708, 712, 2156, 2285
HgaI	GACGC	4 cut(s)	263, 356, 1677, 1842
HhaI	GCGC	4 cut(s)	56, 263, 294, 357
Hin1I	GRCGYC	3 cut(s)	255, 348, 1688
Hin1II	CATG	7 cut(s)	232, 730, 950, 1027, 1517, 1739, 2356
Hin6I	GCGC	4 cut(s)	54, 261, 292, 355
HinP1I	GCGC	4 cut(s)	54, 261, 292, 355
HincII	GTYRAC	4 cut(s)	34, 152, 1270, 1857
HindII	GTYRAC	4 cut(s)	34, 152, 1270, 1857
HindIII	AAGCTT	1 cut(s)	2036
HinfI	GANTC	7 cut(s)	197, 275, 463, 1063, 1346, 2186, 2300
HpaII	CCGG	6 cut(s)	300, 456, 708, 712, 2156, 2285
HphI	GGTGA	3 cut(s)	875, 1001, 1830
Hpy166II	GTNNAC	8 cut(s)	34, 152, 755, 1270, 1508, 1857, 2062, 2083
Hpy188III	TCNNGA	6 cut(s)	456, 521, 929, 1242, 1470, 2009
Hpy8I	GTNNAC	8 cut(s)	34, 152, 755, 1270, 1508, 1857, 2062, 2083
Hpy99I	CGWCG	3 cut(s)	112, 556, 2258
HpyCH4III	ACNGT	4 cut(s)	79, 752, 1189, 2087
HpyCH4IV	ACGT	7 cut(s)	372, 582, 1171, 1452, 1541, 1584, 1648
HpyCH4V	TGCA	9 cut(s)	363, 687, 702, 830, 946, 1307, 1513, 1616, 2356
HpyF10VI	GCNNNNNNNGC	9 cut(s)	51, 267, 708, 712, 721, 850, 865, 1529, 1967
HpyF3I	CTNAG	9 cut(s)	159, 250, 333, 519, 797, 897, 938, 1192, 2039
HpySE526I	ACGT	7 cut(s)	372, 582, 1171, 1452, 1541, 1584, 1648
Hsp92I	GRCGYC	3 cut(s)	255, 348, 1688
Hsp92II	CATG	7 cut(s)	232, 730, 950, 1027, 1517, 1739, 2356
HspAI	GCGC	4 cut(s)	54, 261, 292, 355
Kpn2I	TCCGGA	1 cut(s)	455
KroI	GCCGGC	1 cut(s)	711
KroNI	GCCGGC	1 cut(s)	713
LguI	GCTCTTC	1 cut(s)	875
LmnI	GCTCC	8 cut(s)	168, 224, 458, 670, 1032, 1744, 1762, 2152
Lsp1109I	GCAGC	5 cut(s)	309, 427, 546, 607, 1734
LweI	GCATC	7 cut(s)	150, 546, 883, 1316, 1365, 1625, 1640
MabI	ACCWGGT	1 cut(s)	308
MaeI	CTAG	4 cut(s)	1067, 1628, 1782, 1958
MaeII	ACGT	7 cut(s)	372, 582, 1171, 1452, 1541, 1584, 1648
MaeIII	GTNAC	5 cut(s)	958, 1202, 1555, 1585, 1784
MfeI	CAATTG	2 cut(s)	483, 1599
MflI	RGATCY	3 cut(s)	901, 925, 1804
MhlI	GDGCHC	4 cut(s)	165, 675, 1525, 1534
MlyI	GAGTC	3 cut(s)	269, 457, 2294
MmeI	TCCRAC	4 cut(s)	246, 575, 1143, 1838
MroI	TCCGGA	1 cut(s)	455
MroNI	GCCGGC	1 cut(s)	711
MseI	TTAA	7 cut(s)	15, 813, 1116, 1536, 1655, 1886, 2345
MslI	CAYNNNNRTG	1 cut(s)	729
MspA1I	CMGCKG	2 cut(s)	322, 1829
MspCI	CTTAAG	1 cut(s)	14
MspI	CCGG	6 cut(s)	300, 456, 708, 712, 2156, 2285
MspR9I	CCNGG	8 cut(s)	310, 708, 1060, 1141, 1262, 1296, 1503, 2286
MunI	CAATTG	2 cut(s)	483, 1599
Mva1269I	GAATGC	1 cut(s)	832
MvaI	CCWGG	6 cut(s)	310, 1060, 1141, 1262, 1296, 1503
MvnI	CGCG	5 cut(s)	54, 216, 261, 292, 435
MwoI	GCNNNNNNNGC	9 cut(s)	51, 267, 708, 712, 721, 850, 865, 1529, 1967
NaeI	GCCGGC	1 cut(s)	713
NciI	CCSGG	2 cut(s)	708, 2286
NgoMIV	GCCGGC	1 cut(s)	711
NlaIII	CATG	7 cut(s)	232, 730, 950, 1027, 1517, 1739, 2356
NlaIV	GGNNCC	6 cut(s)	220, 304, 454, 605, 927, 2154
NmeAIII	GCCGAG	1 cut(s)	854
NmuCI	GTSAC	2 cut(s)	958, 1784
NspI	RCATGY	2 cut(s)	1027, 1517
NspV	TTCGAA	1 cut(s)	1868
PaeI	GCATGC	1 cut(s)	1517
PceI	AGGCCT	1 cut(s)	1248
PciI	ACATGT	1 cut(s)	1023
PciSI	GCTCTTC	1 cut(s)	875
PctI	GAATGC	1 cut(s)	832
PdiI	GCCGGC	1 cut(s)	713
PfeI	GAWTC	4 cut(s)	197, 1063, 1346, 2186
PflMI	CCANNNNNTGG	1 cut(s)	1741
PfoI	TCCNGGA	2 cut(s)	1058, 1294
Ple19I	CGATCG	1 cut(s)	2212
PleI	GAGTC	3 cut(s)	269, 457, 2294
PpsI	GAGTC	3 cut(s)	269, 457, 2294
PscI	ACATGT	1 cut(s)	1023
PsiI	TTATAA	1 cut(s)	2031
Psp124BI	GAGCTC	2 cut(s)	165, 675
Psp6I	CCWGG	6 cut(s)	308, 1058, 1139, 1260, 1294, 1501
PspFI	CCCAGC	1 cut(s)	201
PspGI	CCWGG	6 cut(s)	308, 1058, 1139, 1260, 1294, 1501
PspN4I	GGNNCC	6 cut(s)	220, 304, 454, 605, 927, 2154
PspPI	GGNCC	1 cut(s)	1732
PstNI	CAGNNNCTG	1 cut(s)	1469
PsuI	RGATCY	3 cut(s)	901, 925, 1804
PvuI	CGATCG	1 cut(s)	2212
PvuII	CAGCTG	1 cut(s)	322
RsaI	GTAC	6 cut(s)	371, 500, 1095, 1218, 1499, 1849
RsaNI	GTAC	6 cut(s)	370, 499, 1094, 1217, 1498, 1848
RseI	CAYNNNNRTG	1 cut(s)	729
SacI	GAGCTC	2 cut(s)	165, 675
SapI	GCTCTTC	1 cut(s)	875
SaqAI	TTAA	7 cut(s)	15, 813, 1116, 1536, 1655, 1886, 2345
Sau96I	GGNCC	1 cut(s)	1732
SchI	GAGTC	3 cut(s)	269, 457, 2294
ScrFI	CCNGG	8 cut(s)	310, 708, 1060, 1141, 1262, 1296, 1503, 2286
SduI	GDGCHC	4 cut(s)	165, 675, 1525, 1534
SexAI	ACCWGGT	1 cut(s)	308
SfaNI	GCATC	7 cut(s)	150, 546, 883, 1316, 1365, 1625, 1640
SfuI	TTCGAA	1 cut(s)	1868
SgrAI	CRCCGGYG	1 cut(s)	299
SinI	GGWCC	1 cut(s)	1732
SmiMI	CAYNNNNRTG	1 cut(s)	729
SmlI	CTYRAG	2 cut(s)	14, 1397
SmoI	CTYRAG	2 cut(s)	14, 1397
SphI	GCATGC	1 cut(s)	1517
SseBI	AGGCCT	1 cut(s)	1248
SspI	AATATT	1 cut(s)	2027
SspMI	CTAG	4 cut(s)	1067, 1628, 1782, 1958
SstI	GAGCTC	2 cut(s)	165, 675
StuI	AGGCCT	1 cut(s)	1248
StyD4I	CCNGG	8 cut(s)	308, 706, 1058, 1139, 1260, 1294, 1501, 2284
StyI	CCWWGG	1 cut(s)	342
TaaI	ACNGT	4 cut(s)	79, 752, 1189, 2087
TaiI	ACGT	7 cut(s)	375, 585, 1174, 1455, 1544, 1587, 1651
TatI	WGTACW	3 cut(s)	498, 1093, 1847
TauI	GCSGC	7 cut(s)	45, 219, 270, 423, 985, 1415, 2097
TfiI	GAWTC	4 cut(s)	197, 1063, 1346, 2186
Tru1I	TTAA	7 cut(s)	15, 813, 1116, 1536, 1655, 1886, 2345
Tru9I	TTAA	7 cut(s)	15, 813, 1116, 1536, 1655, 1886, 2345
TscAI	CASTG	3 cut(s)	1470, 1914, 1993
TseFI	GTSAC	2 cut(s)	958, 1784
TseI	GCWGC	5 cut(s)	322, 440, 534, 620, 1747
Tsp45I	GTSAC	2 cut(s)	958, 1784
TspDTI	ATGAA	5 cut(s)	56, 747, 839, 1027, 1752
TspGWI	ACGGA	1 cut(s)	1204
TspRI	CASTG	3 cut(s)	1470, 1914, 1993
Van91I	CCANNNNNTGG	1 cut(s)	1741
Vha464I	CTTAAG	1 cut(s)	14
VpaK11BI	GGWCC	1 cut(s)	1732
XagI	CCTNNNNNAGG	1 cut(s)	779
XapI	RAATTY	3 cut(s)	932, 1483, 2004
XceI	RCATGY	2 cut(s)	1027, 1517
XmiI	GTMKAC	1 cut(s)	1507
XspI	CTAG	4 cut(s)	1067, 1628, 1782, 1958

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.