Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0002471

Physical Location & Seq

Genomic Coordinates Reverse (-)

948846 .. 953081

Gene Structure 4236 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005702

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2235 bp

ATGAGAAAACCCATATCAAAAACAACGCGGAAGCAAAAGCTACTCTCGGAGGTCGAAGAAATCAACCTCCTCAATTCATGGATCGAATCCCAGAAACCCGACTGCGGCTCCAACCCCATGTCTCTCCCGCCTCTTCCCTCCAAGGCCCCCATTGGGCGCGTGGACCCCACCTCCTTCTCCCGCTACGCCGGCGCCACCAGGTTCGCCGACCTCCCCATCTCCAAGAAGACCAAGGACGCCTTGCGCCAGTCCAAGTACACCGACATGACCGATATTCAGAGGGCGTGTTTGCCCCACGCGCTCTGCGGCAGGGATATTCTCGGCGCCGCCAAGACCGGCTCCGGCAAGACTCTGGCTTTTGTGATTCCGCTTCTGGAAAAATTGTATAAAGACAGATGGAATCCTGAGGATGGGGTAGGCAGCATTATTATATCTCCAACAAGGGAATTAGCTAGTCAAACGTTTGATGTGTTAAGAGCAGTTGGGAAGCACCATAATTTTAGTGCTGGCCTCCTAATTGGTGGTCGAAAAGATGTTGAAGCGGAGAAGGAACGTGTAAATGAACTGAACATACTGGTTTGCACTCCTGGACGACTTCTACAACACATGGATGAGACACCAAATTTTGAGTGTTCACAGCTACAGGTTTTGGTCCTTGATGAGGCAGATCGTATTCTTGATGTGGGATTTAAGAAGACACTGAACGCCATCATTTCACAGCTACCTAAGAGAAGACAAACCTTTCTCTTCTCTGCAACTCAAACAAAGTCGGTTCAAGATCTTGCAAGACTTAGTTTGAAGGACCCAGAGTACCTTAGCGTGCATGAGAAAGCTACAAGAGCCACTCCTAATCGTTTGAAGCAAACTGCAATGATTGTTCCTCTTGATAAAAAACTAGATATGCTGTGGAGCTTCATAAAGGCGCATCTGAATTCTAGGATTTTAGTGTTCTTATCGACACGTAAACAGGTAAAGTTTGTCTATGAAGCATTTAAGAAGCTCCGTCCTGGAATACCCCTGAAGTGTCTTCATGGTAAGATGAAGCAAGAGGGAAGAATGGGAACATACTCGCAGTTTCATGAAAAAGCTACGGTCCTTTTCTCGACTGATGTGGCTTCGAGAGGCCTTGATTTCAACCAGGCAGTCGACTGGGTTGTTCAGGTGGATTGTCCAGAAGATGTTGCATCCTATATACACAGAGTTGGCCGAACTGCGCGCTATCAATCAGAAGGGCGATCACTTTTATTTGTGATGCCCTCAGAAACAAAAATGCTTGAGAAATTAGATGCCGCAAAAATACCCATAGAAAGCATCAAGGCCAACAACAAGAGACTGCAACCTGTCAGTGGGTTGCTATCAGCTTTGCTAGTCAAGTACCCAGACACACTTCCTGGTCTGGCCAAAAGGGCCTTTATCACGTACTTGAAGTCTATTCAAAATCACAAGGATAAAGAAGTTTTTGACGTAATGCAACTGCCAGTTGATGAGTTTTCAGCATCAGTGGGTTTACCAATGACCCCCAAGATCCGTTTTTCAAACCACAAAATGAAGTCCAAGAAACCATTGGAACTGTCTCCTATTGTTGAACCAGAAAGTTCCGATATGGAGAATGCATTAGAGACCCCCAGAGAGGAGCTTGATATTGGTGATTCGGAGGAAGAGGAAGTGGAGAAAGGTCTTCTTCAAATAAATGATGCACCAACTGAGAGAGAGGAGGAAGGAAGACAGATTGCAGATATTATGCCGGCAACTCGGGTTCTGAAGAAAAAGAAGTTGAAGATCAATCTCAATAGACCATTGGGAACTAGGAAGGTCTATGATGAGGATGGCAATTTGGTGGACCCACTTGCAAGGCTGGCTGGTCCCAGTGATTCTAGCATTCTTGATGGACTTCAAGACAAGAAAGAAGAATTTCATAAGAGGCTTAGGGAAGAGGTGAAGATGGTAGACAAGGAAGATAAGGTAAAAGATCGCCAACGTCGCCGTGAAAAACGAATGAAGGAGAAGATGAAGTTGAAGAGAGCAGATGCAGAAGAAGAGGAGGATGATGATTCTGAGTCGGAAGAAGAGCCATCTGATAATCGATCGCACAAAAGGTCAAAGATTTACTTTGATAGTGATGATAATGGTGAGAGGGAAGAAAAGAAGGATAAAGCTGGGTTCATTGCTGACCCAAGTTATATAGAGCATCAGCAAGCTTTGGCTCTCAAGCTGTTAGATTCTATGCACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

745

Amino Acids

84.48

Weight (kDa)

8.86

Isoelectric Point (pI)

52.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	90 - 261	6e-45	DEAD/DEAH box helicase
ResIII	PF04851	109 - 253	5.6e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	298 - 407	8.8e-24	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	466 - 509	2.1e-09	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002471)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54910
fragaria_vesca	FvH4_4g36230 FvH4_4g36230 FvH4_4g36230 FvH4_5g34980
malus_domestica	MD08G1022200.v1.1 MD13G1010800.v1.1 MD15G1026900.v1.1
prunus_persica	Prupe.1G342200_v2.0.a1
pyrus_communis	pycom08g13590 pycom13g00910 pycom15g02270 pycom15g02280 pycom15g02290
rosa_chinensis	RchiOBHm_Chr1g0336991 RchiOBHm_Chr4g0445981
rosa_laevigata	RLG00000005702 RLG00000029316
rosa_multiflora	Rmu_co8191210.1_g000001 Rmu_sc0000115.1_g000004 Rmu_sc0003001.1_g000004 Rmu_sc0008543.1_g000002 Rmu_sc0009742.1_g000002 Rmu_sc0036469.1_g000003
rosa_roxburghii	Rroxscaffold_4G00315110 Rroxscaffold_5G00386390
rosa_rugosa	Rorug01G0129500 Rorug01G0130100 Rorug04G0366700 Rorug04G0366700
rosa_samantha	Rh1AG150500 Rh1BG118700 Rh1BG118900 Rh1CG141800 Rh1DG157800 Rh4AG428100 Rh4BG437700 Rh4CG454400 Rh4DG436200
rosa_wichuraiana	Rw1G012290 Rw4G036610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	191, 323
AccI	GTMKAC	2 cut(s)	1146, 1947
AccII	CGCG	4 cut(s)	28, 159, 299, 1216
AciI	CCGC	9 cut(s)	28, 105, 128, 181, 306, 327, 368, 542, 1290
AclI	AACGTT	1 cut(s)	461
AclWI	GGATC	2 cut(s)	89, 1519
AcoI	YGGCCR	2 cut(s)	1204, 1398
AcsI	RAATTY	3 cut(s)	622, 931, 1910
AcuI	CTGAAG	2 cut(s)	1040, 1781
AcyI	GRCGYC	3 cut(s)	192, 237, 324
AfaI	GTAC	4 cut(s)	257, 812, 1376, 1421
AfiI	CCNNNNNNNGG	6 cut(s)	104, 153, 410, 661, 1346, 1630
AflIII	ACRYGT	2 cut(s)	553, 959
AjnI	CCWGG	5 cut(s)	197, 586, 1006, 1137, 1390
Alw26I	GTCTC	5 cut(s)	126, 608, 1324, 1578, 1613
AlwI	GGATC	2 cut(s)	89, 1519
Ama87I	CYCGRG	1 cut(s)	1752
AoxI	GGCC	7 cut(s)	144, 508, 1123, 1204, 1317, 1398, 1407
ApeKI	GCWGC	1 cut(s)	420
ApoI	RAATTY	3 cut(s)	622, 931, 1910
Asp700I	GAANNNNTTC	1 cut(s)	1911
AspLEI	GCGC	8 cut(s)	159, 194, 246, 301, 326, 925, 1216, 1218
AspS9I	GGNCC	8 cut(s)	145, 163, 652, 802, 1093, 1407, 1840, 1862
AsuHPI	GGTGA	3 cut(s)	1658, 1948, 2141
AvaI	CYCGRG	1 cut(s)	1752
AvaII	GGWCC	6 cut(s)	163, 652, 802, 1093, 1840, 1862
AxyI	CCTNAGG	1 cut(s)	405
BalI	TGGCCA	1 cut(s)	1400
BanI	GGYRCC	2 cut(s)	191, 323
BbsI	GAAGAC	6 cut(s)	233, 701, 739, 1019, 1670, 1729
BbvI	GCAGC	1 cut(s)	432
BccI	CCATC	8 cut(s)	224, 390, 404, 716, 1820, 1880, 1936, 2080
BceAI	ACGGC	1 cut(s)	1968
BciT130I	CCWGG	5 cut(s)	199, 588, 1008, 1139, 1392
BcoDI	GTCTC	5 cut(s)	126, 608, 1324, 1578, 1613
BfaI	CTAG	6 cut(s)	453, 896, 936, 1367, 1806, 1875
BfmI	CTRYAG	1 cut(s)	641
BfoI	RGCGCY	2 cut(s)	195, 327
BglI	GCCNNNNNGGC	1 cut(s)	1406
BglII	AGATCT	1 cut(s)	778
BisI	GCNGC	5 cut(s)	106, 307, 327, 421, 1290
BlsI	GCNGC	5 cut(s)	107, 308, 328, 422, 1291
Bme1390I	CCNGG	5 cut(s)	199, 588, 1008, 1139, 1392
Bme18I	GGWCC	6 cut(s)	163, 652, 802, 1093, 1840, 1862
BmeT110I	CYCGRG	1 cut(s)	1752
BmgT120I	GGNCC	8 cut(s)	145, 163, 652, 802, 1093, 1407, 1840, 1862
BmiI	GGNNCC	9 cut(s)	109, 147, 165, 193, 325, 340, 804, 1842, 1864
BmrFI	CCNGG	5 cut(s)	199, 588, 1008, 1139, 1392
BmrI	ACTGGG	2 cut(s)	1159, 1860
BmsI	GCATC	9 cut(s)	934, 1193, 1242, 1276, 1320, 1505, 1684, 2017, 2197
BmuI	ACTGGG	2 cut(s)	1159, 1860
BpiI	GAAGAC	6 cut(s)	233, 701, 739, 1019, 1670, 1729
Bpu10I	CCTNAGC	2 cut(s)	815, 1925
BpuEI	CTTGAG	2 cut(s)	1295, 2192
Bsa29I	ATCGAT	1 cut(s)	2083
BsaAI	YACGTR	2 cut(s)	962, 1419
BsaHI	GRCGYC	3 cut(s)	192, 237, 324
BsaI	GGTCTC	1 cut(s)	1613
BsaJI	CCNNGG	2 cut(s)	141, 231
BsaXI	ACNNNNNCTCC	4 cut(s)	92, 122, 155, 185
Bsc4I	CCNNNNNNNGG	6 cut(s)	104, 153, 410, 661, 1346, 1630
Bse118I	RCCGGY	3 cut(s)	188, 335, 1744
Bse1I	ACTGG	5 cut(s)	247, 579, 1154, 1478, 1866
Bse21I	CCTNAGG	1 cut(s)	405
Bse3DI	GCAATG	2 cut(s)	876, 2163
BseBI	CCWGG	5 cut(s)	199, 588, 1008, 1139, 1392
BseCI	ATCGAT	1 cut(s)	2083
BseDI	CCNNGG	2 cut(s)	141, 231
BseGI	GGATG	5 cut(s)	415, 616, 1184, 1831, 2050
BseLI	CCNNNNNNNGG	6 cut(s)	104, 153, 410, 661, 1346, 1630
BseMI	GCAATG	2 cut(s)	876, 2163
BseMII	CTCAG	4 cut(s)	396, 1272, 1695, 2046
BseNI	ACTGG	5 cut(s)	247, 579, 1154, 1478, 1866
BsePI	GCGCGC	1 cut(s)	1214
BseRI	GAGGAG	4 cut(s)	59, 1646, 1727, 2054
BseXI	GCAGC	1 cut(s)	432
BseYI	CCCAGC	1 cut(s)	2156
Bsh1236I	CGCG	4 cut(s)	28, 159, 299, 1216
Bsh1285I	CGRYCG	1 cut(s)	2087
BshFI	GGCC	7 cut(s)	146, 510, 1125, 1206, 1319, 1400, 1409
BshNI	GGYRCC	2 cut(s)	191, 323
BshVI	ATCGAT	1 cut(s)	2083
BsiEI	CGRYCG	1 cut(s)	2087
BsiHKCI	CYCGRG	1 cut(s)	1752
BsiSI	CCGG	4 cut(s)	189, 336, 342, 1745
BslFI	GGGAC	1 cut(s)	1848
BslI	CCNNNNNNNGG	6 cut(s)	104, 153, 410, 661, 1346, 1630
BsmAI	GTCTC	5 cut(s)	126, 608, 1324, 1578, 1613
BsmFI	GGGAC	1 cut(s)	1848
BsmI	GAATGC	2 cut(s)	1615, 1878
BsnI	GGCC	7 cut(s)	146, 510, 1125, 1206, 1319, 1400, 1409
Bso31I	GGTCTC	1 cut(s)	1613
BsoBI	CYCGRG	1 cut(s)	1752
Bsp143I	GATC	8 cut(s)	81, 667, 778, 1235, 1524, 1779, 1969, 2084
BspACI	CCGC	9 cut(s)	28, 105, 128, 181, 306, 327, 368, 542, 1290
BspANI	GGCC	7 cut(s)	146, 510, 1125, 1206, 1319, 1400, 1409
BspCNI	CTCAG	4 cut(s)	397, 1271, 1696, 2047
BspDI	ATCGAT	1 cut(s)	2083
BspFNI	CGCG	4 cut(s)	28, 159, 299, 1216
BspHI	TCATGA	1 cut(s)	1078
BspLI	GGNNCC	9 cut(s)	109, 147, 165, 193, 325, 340, 804, 1842, 1864
BspPI	GGATC	2 cut(s)	89, 1519
BspQI	GCTCTTC	1 cut(s)	2061
BspT107I	GGYRCC	2 cut(s)	191, 323
BspTNI	GGTCTC	1 cut(s)	1613
BsrDI	GCAATG	2 cut(s)	876, 2163
BsrFI	RCCGGY	3 cut(s)	188, 335, 1744
BsrI	ACTGG	5 cut(s)	247, 579, 1154, 1478, 1866
BssAI	RCCGGY	3 cut(s)	188, 335, 1744
BssECI	CCNNGG	2 cut(s)	141, 231
BssHII	GCGCGC	1 cut(s)	1214
BssMI	GATC	8 cut(s)	81, 667, 778, 1235, 1524, 1779, 1969, 2084
BssNI	GRCGYC	3 cut(s)	192, 237, 324
BssT1I	CCWWGG	2 cut(s)	141, 231
Bst2UI	CCWGG	5 cut(s)	199, 588, 1008, 1139, 1392
Bst4CI	ACNGT	2 cut(s)	1093, 1572
Bst6I	CTCTTC	7 cut(s)	138, 752, 1653, 1926, 2012, 2031, 2061
BstACI	GRCGYC	3 cut(s)	192, 237, 324
BstBAI	YACGTR	2 cut(s)	962, 1419
BstC8I	GCNNGC	7 cut(s)	190, 508, 821, 1216, 1746, 1857, 2196
BstDEI	CTNAG	8 cut(s)	405, 726, 791, 815, 1258, 1704, 1925, 2055
BstENI	CCTNNNNNAGG	1 cut(s)	659
BstF5I	GGATG	5 cut(s)	415, 616, 1184, 1831, 2050
BstFNI	CGCG	4 cut(s)	28, 159, 299, 1216
BstH2I	RGCGCY	2 cut(s)	195, 327
BstHHI	GCGC	8 cut(s)	159, 194, 246, 301, 326, 925, 1216, 1218
BstKTI	GATC	8 cut(s)	84, 670, 781, 1238, 1527, 1782, 1972, 2087
BstMAI	GTCTC	5 cut(s)	126, 608, 1324, 1578, 1613
BstMBI	GATC	8 cut(s)	81, 667, 778, 1235, 1524, 1779, 1969, 2084
BstMCI	CGRYCG	1 cut(s)	2087
BstMWI	GCNNNNNNNGC	6 cut(s)	189, 298, 839, 1406, 1856, 1980
BstNI	CCWGG	5 cut(s)	199, 588, 1008, 1139, 1392
BstSCI	CCNGG	5 cut(s)	197, 586, 1006, 1137, 1390
BstSFI	CTRYAG	1 cut(s)	641
BstUI	CGCG	4 cut(s)	28, 159, 299, 1216
BstV1I	GCAGC	1 cut(s)	432
BstV2I	GAAGAC	6 cut(s)	233, 701, 739, 1019, 1670, 1729
BstX2I	RGATCY	2 cut(s)	778, 1524
BstYI	RGATCY	2 cut(s)	778, 1524
Bsu15I	ATCGAT	1 cut(s)	2083
Bsu36I	CCTNAGG	1 cut(s)	405
BsuRI	GGCC	7 cut(s)	146, 510, 1125, 1206, 1319, 1400, 1409
BsuTUI	ATCGAT	1 cut(s)	2083
BtsCI	GGATG	5 cut(s)	415, 616, 1184, 1831, 2050
BtsIMutI	CAGTG	4 cut(s)	698, 1351, 1506, 1873
Cac8I	GCNNGC	7 cut(s)	190, 508, 821, 1216, 1746, 1857, 2196
CciI	TCATGA	1 cut(s)	1078
CfoI	GCGC	8 cut(s)	159, 194, 246, 301, 326, 925, 1216, 1218
Cfr10I	RCCGGY	3 cut(s)	188, 335, 1744
Cfr13I	GGNCC	8 cut(s)	145, 163, 652, 802, 1093, 1407, 1840, 1862
ClaI	ATCGAT	1 cut(s)	2083
CseI	GACGC	1 cut(s)	245
CsiI	ACCWGGT	1 cut(s)	197
Csp6I	GTAC	4 cut(s)	256, 811, 1375, 1420
CviAII	CATG	7 cut(s)	78, 118, 265, 607, 824, 1031, 1079
CviQI	GTAC	4 cut(s)	256, 811, 1375, 1420
DdeI	CTNAG	8 cut(s)	405, 726, 791, 815, 1258, 1704, 1925, 2055
DinI	GGCGCC	2 cut(s)	193, 325
DpnI	GATC	8 cut(s)	83, 669, 780, 1237, 1526, 1781, 1971, 2086
DpnII	GATC	8 cut(s)	81, 667, 778, 1235, 1524, 1779, 1969, 2084
EaeI	YGGCCR	2 cut(s)	1204, 1398
Eam1104I	CTCTTC	7 cut(s)	138, 752, 1653, 1926, 2012, 2031, 2061
EarI	CTCTTC	7 cut(s)	138, 752, 1653, 1926, 2012, 2031, 2061
Eco130I	CCWWGG	2 cut(s)	141, 231
Eco147I	AGGCCT	1 cut(s)	1125
Eco31I	GGTCTC	1 cut(s)	1613
Eco47I	GGWCC	6 cut(s)	163, 652, 802, 1093, 1840, 1862
Eco57I	CTGAAG	2 cut(s)	1040, 1781
Eco81I	CCTNAGG	1 cut(s)	405
Eco88I	CYCGRG	1 cut(s)	1752
EcoNI	CCTNNNNNAGG	1 cut(s)	659
EcoO109I	RGGNCCY	3 cut(s)	145, 802, 1407
EcoRI	GAATTC	1 cut(s)	931
EcoRII	CCWGG	5 cut(s)	197, 586, 1006, 1137, 1390
EcoT14I	CCWWGG	2 cut(s)	141, 231
EcoT22I	ATGCAT	1 cut(s)	1615
EgeI	GGCGCC	2 cut(s)	193, 325
EheI	GGCGCC	2 cut(s)	193, 325
ErhI	CCWWGG	2 cut(s)	141, 231
FaeI	CATG	7 cut(s)	81, 121, 268, 610, 827, 1034, 1082
FalI	AAGNNNNNCTT	6 cut(s)	224, 256, 1665, 1697, 2093, 2125
FaqI	GGGAC	1 cut(s)	1848
FatI	CATG	7 cut(s)	77, 117, 264, 606, 823, 1030, 1078
FauI	CCCGC	2 cut(s)	135, 188
FblI	GTMKAC	2 cut(s)	1146, 1947
Fnu4HI	GCNGC	5 cut(s)	106, 307, 327, 421, 1290
FokI	GGATG	5 cut(s)	422, 623, 1171, 1838, 2057
Fsp4HI	GCNGC	5 cut(s)	106, 307, 327, 421, 1290
FspBI	CTAG	6 cut(s)	453, 896, 936, 1367, 1806, 1875
GlaI	GCGC	8 cut(s)	158, 193, 245, 300, 325, 924, 1215, 1217
GluI	GCNGC	5 cut(s)	106, 307, 327, 421, 1290
GsaI	CCCAGC	1 cut(s)	2160
HaeII	RGCGCY	2 cut(s)	195, 327
HaeIII	GGCC	7 cut(s)	146, 510, 1125, 1206, 1319, 1400, 1409
HapII	CCGG	4 cut(s)	189, 336, 342, 1745
HgaI	GACGC	1 cut(s)	245
HhaI	GCGC	8 cut(s)	159, 194, 246, 301, 326, 925, 1216, 1218
Hin1I	GRCGYC	3 cut(s)	192, 237, 324
Hin1II	CATG	7 cut(s)	81, 121, 268, 610, 827, 1034, 1082
Hin6I	GCGC	8 cut(s)	157, 192, 244, 299, 324, 923, 1214, 1216
HinP1I	GCGC	8 cut(s)	157, 192, 244, 299, 324, 923, 1214, 1216
HincII	GTYRAC	1 cut(s)	1147
HindII	GTYRAC	1 cut(s)	1147
HindIII	AAGCTT	1 cut(s)	2196
HinfI	GANTC	9 cut(s)	86, 349, 364, 400, 1649, 1871, 2051, 2057, 2219
HpaII	CCGG	4 cut(s)	189, 336, 342, 1745
HphI	GGTGA	3 cut(s)	1658, 1948, 2141
Hpy166II	GTNNAC	8 cut(s)	163, 258, 635, 965, 1147, 1508, 1840, 1948
Hpy8I	GTNNAC	8 cut(s)	163, 258, 635, 965, 1147, 1508, 1840, 1948
Hpy99I	CGWCG	1 cut(s)	1983
HpyAV	CCTTC	8 cut(s)	184, 541, 793, 1223, 1712, 1804, 1993, 2140
HpyCH4III	ACNGT	2 cut(s)	1093, 1572
HpyCH4IV	ACGT	6 cut(s)	461, 553, 961, 1418, 1464, 1978
HpyF10VI	GCNNNNNNNGC	6 cut(s)	189, 298, 839, 1406, 1856, 1980
HpyF3I	CTNAG	8 cut(s)	405, 726, 791, 815, 1258, 1704, 1925, 2055
HpySE526I	ACGT	6 cut(s)	461, 553, 961, 1418, 1464, 1978
Hsp92I	GRCGYC	3 cut(s)	192, 237, 324
Hsp92II	CATG	7 cut(s)	81, 121, 268, 610, 827, 1034, 1082
HspAI	GCGC	8 cut(s)	157, 192, 244, 299, 324, 923, 1214, 1216
KasI	GGCGCC	2 cut(s)	191, 323
KroI	GCCGGC	2 cut(s)	188, 1744
KroNI	GCCGGC	2 cut(s)	190, 1746
Kzo9I	GATC	8 cut(s)	81, 667, 778, 1235, 1524, 1779, 1969, 2084
LguI	GCTCTTC	1 cut(s)	2061
LmnI	GCTCC	5 cut(s)	113, 344, 909, 1005, 1633
Lsp1109I	GCAGC	1 cut(s)	432
LweI	GCATC	9 cut(s)	934, 1193, 1242, 1276, 1320, 1505, 1684, 2017, 2197
MabI	ACCWGGT	1 cut(s)	197
MaeI	CTAG	6 cut(s)	453, 896, 936, 1367, 1806, 1875
MaeII	ACGT	6 cut(s)	461, 553, 961, 1418, 1464, 1978
MalI	GATC	8 cut(s)	83, 669, 780, 1237, 1526, 1781, 1971, 2086
MboI	GATC	8 cut(s)	81, 667, 778, 1235, 1524, 1779, 1969, 2084
MflI	RGATCY	2 cut(s)	778, 1524
MlsI	TGGCCA	1 cut(s)	1400
MluNI	TGGCCA	1 cut(s)	1400
Mly113I	GGCGCC	2 cut(s)	192, 324
MlyI	GAGTC	2 cut(s)	343, 2066
MmeI	TCCRAC	3 cut(s)	135, 461, 2040
Mox20I	TGGCCA	1 cut(s)	1400
Mph1103I	ATGCAT	1 cut(s)	1615
MreI	CGCCGGCG	1 cut(s)	188
MroNI	GCCGGC	2 cut(s)	188, 1744
MroXI	GAANNNNTTC	1 cut(s)	1911
MscI	TGGCCA	1 cut(s)	1400
MseI	TTAA	3 cut(s)	473, 690, 993
MslI	CAYNNNNRTG	2 cut(s)	263, 609
Msp20I	TGGCCA	1 cut(s)	1400
MspI	CCGG	4 cut(s)	189, 336, 342, 1745
MspR9I	CCNGG	5 cut(s)	199, 588, 1008, 1139, 1392
Mva1269I	GAATGC	2 cut(s)	1615, 1878
MvaI	CCWGG	5 cut(s)	199, 588, 1008, 1139, 1392
MvnI	CGCG	4 cut(s)	28, 159, 299, 1216
MwoI	GCNNNNNNNGC	6 cut(s)	189, 298, 839, 1406, 1856, 1980
NaeI	GCCGGC	2 cut(s)	190, 1746
NarI	GGCGCC	2 cut(s)	192, 324
NdeII	GATC	8 cut(s)	81, 667, 778, 1235, 1524, 1779, 1969, 2084
NgoMIV	GCCGGC	2 cut(s)	188, 1744
NlaIII	CATG	7 cut(s)	81, 121, 268, 610, 827, 1034, 1082
NlaIV	GGNNCC	9 cut(s)	109, 147, 165, 193, 325, 340, 804, 1842, 1864
NmeAIII	GCCGAG	1 cut(s)	300
NsiI	ATGCAT	1 cut(s)	1615
PagI	TCATGA	1 cut(s)	1078
PauI	GCGCGC	1 cut(s)	1214
PceI	AGGCCT	1 cut(s)	1125
PciSI	GCTCTTC	1 cut(s)	2061
PctI	GAATGC	2 cut(s)	1615, 1878
PdiI	GCCGGC	2 cut(s)	190, 1746
PdmI	GAANNNNTTC	1 cut(s)	1911
PfeI	GAWTC	7 cut(s)	86, 364, 400, 1649, 1871, 2051, 2219
PfoI	TCCNGGA	2 cut(s)	586, 1006
PkrI	GCNGC	5 cut(s)	107, 308, 328, 422, 1291
Ple19I	CGATCG	1 cut(s)	2087
PleI	GAGTC	2 cut(s)	343, 2065
PluTI	GGCGCC	2 cut(s)	195, 327
PpsI	GAGTC	2 cut(s)	343, 2065
Ppu21I	YACGTR	2 cut(s)	962, 1419
PpuMI	RGGWCCY	1 cut(s)	802
Psp1406I	AACGTT	1 cut(s)	461
Psp5II	RGGWCCY	1 cut(s)	802
Psp6I	CCWGG	5 cut(s)	197, 586, 1006, 1137, 1390
PspFI	CCCAGC	1 cut(s)	2156
PspGI	CCWGG	5 cut(s)	197, 586, 1006, 1137, 1390
PspN4I	GGNNCC	9 cut(s)	109, 147, 165, 193, 325, 340, 804, 1842, 1864
PspPI	GGNCC	8 cut(s)	145, 163, 652, 802, 1093, 1407, 1840, 1862
PspPPI	RGGWCCY	1 cut(s)	802
PsrI	GAACNNNNNNTAC	2 cut(s)	555, 587
PsuI	RGATCY	2 cut(s)	778, 1524
PteI	GCGCGC	1 cut(s)	1214
PvuI	CGATCG	1 cut(s)	2087
RsaI	GTAC	4 cut(s)	257, 812, 1376, 1421
RsaNI	GTAC	4 cut(s)	256, 811, 1375, 1420
RseI	CAYNNNNRTG	2 cut(s)	263, 609
SalI	GTCGAC	1 cut(s)	1145
SapI	GCTCTTC	1 cut(s)	2061
SaqAI	TTAA	3 cut(s)	473, 690, 993
SatI	GCNGC	5 cut(s)	106, 307, 327, 421, 1290
Sau3AI	GATC	8 cut(s)	81, 667, 778, 1235, 1524, 1779, 1969, 2084
Sau96I	GGNCC	8 cut(s)	145, 163, 652, 802, 1093, 1407, 1840, 1862
SchI	GAGTC	2 cut(s)	343, 2066
ScrFI	CCNGG	5 cut(s)	199, 588, 1008, 1139, 1392
SexAI	ACCWGGT	1 cut(s)	197
SfaNI	GCATC	9 cut(s)	934, 1193, 1242, 1276, 1320, 1505, 1684, 2017, 2197
SfcI	CTRYAG	1 cut(s)	641
SfiI	GGCCNNNNNGGCC	1 cut(s)	1406
SfoI	GGCGCC	2 cut(s)	193, 325
SgrAI	CRCCGGYG	1 cut(s)	188
SinI	GGWCC	6 cut(s)	163, 652, 802, 1093, 1840, 1862
SmiMI	CAYNNNNRTG	2 cut(s)	263, 609
SmlI	CTYRAG	2 cut(s)	1274, 2207
SmoI	CTYRAG	2 cut(s)	1274, 2207
SseBI	AGGCCT	1 cut(s)	1125
SsiI	CCGC	9 cut(s)	28, 105, 128, 181, 306, 327, 368, 542, 1290
SspDI	GGCGCC	2 cut(s)	191, 323
SspMI	CTAG	6 cut(s)	453, 896, 936, 1367, 1806, 1875
StuI	AGGCCT	1 cut(s)	1125
StyD4I	CCNGG	5 cut(s)	197, 586, 1006, 1137, 1390
StyI	CCWWGG	2 cut(s)	141, 231
TaaI	ACNGT	2 cut(s)	1093, 1572
TaiI	ACGT	6 cut(s)	464, 556, 964, 1421, 1467, 1981
TaqI	TCGA	8 cut(s)	54, 84, 526, 956, 1103, 1118, 1146, 2083
TaqII	GACCGA	1 cut(s)	284
TatI	WGTACW	1 cut(s)	255
TauI	GCSGC	4 cut(s)	108, 309, 329, 1292
TfiI	GAWTC	7 cut(s)	86, 364, 400, 1649, 1871, 2051, 2219
Tru1I	TTAA	3 cut(s)	473, 690, 993
Tru9I	TTAA	3 cut(s)	473, 690, 993
TscAI	CASTG	4 cut(s)	705, 1351, 1506, 1873
TseI	GCWGC	1 cut(s)	420
TspGWI	ACGGA	2 cut(s)	992, 1517
TspRI	CASTG	4 cut(s)	705, 1351, 1506, 1873
VpaK11BI	GGWCC	6 cut(s)	163, 652, 802, 1093, 1840, 1862
XagI	CCTNNNNNAGG	1 cut(s)	659
XapI	RAATTY	3 cut(s)	622, 931, 1910
XcmI	CCANNNNNNNNNTGG	2 cut(s)	157, 1561
XmiI	GTMKAC	2 cut(s)	1146, 1947
XmnI	GAANNNNTTC	1 cut(s)	1911
XspI	CTAG	6 cut(s)	453, 896, 936, 1367, 1806, 1875
Zsp2I	ATGCAT	1 cut(s)	1615

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.