Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002471

Physical Location & Seq

Genomic Coordinates Reverse (-)

657237 .. 661538

Gene Structure 4302 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1010800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2235 bp

ATGAGAAAACCCAAATCGAAGTGCTCCCGCAAGCAAAAGGACCTCTCGGAGCTCCAGGAAATCGAGCTTCTGAATTCATGGATTGAATCCCAGAAACCCGATTGTGGCTCCAACCCCATGTCTCTCCCCGCCTTGCCCTCCGACGCCCCCGTCGGCCGTCCCGGTGACACCTTATTTTCTCCCTACGCCGGCGGCACCAGATTCGAGCAGCTGCCCGTCTCTAAGAAGACAAAGGACGCCCTGCGCCAGTGCAAGTACGTTGAAATGACAGATATTCAGAGGGCTTCTTTGCCCCATGCGCTGTGTGGCAGAGATATTCTCGGCGCCGCCAAGACCGGGTCGGGAAAGACTTTGGCTTTTGTTATTCCGCTTGTGGAAAAATTGTATAGAGAAAGATGGGGACCTCAGGATGGGGTAGGCAGCATCATAATATCTCCAACAAGGGAGATAGCTGACCAAACTTTTGATGTGTTAAAATTGGTTGGGAGGCACCATAACTTTAGTGCTGGTCTTCTAATTGGTGGTCGCAAAGTTGAAGAAGAGAAGGAGCACGTAAATGACCTGAACATACTGGTTTGCACTCCAGGACGGCTTCTTCAGCACATGGATGAAACCCCAAATTTTGATTGCTCACAACTTCAGGTTTTGGTCCTTGATGAGGCAGATCGTATTCTTGATGTCGGATTTAAGGACACACTGAATGCAATCATTTCACAGCTACCTAAAAAAAGACAAACGTTTCTCTTCTCAGCAACTCAAACAAAGTCGGTTAAAGATCTTGCAAGACTCAGTTTGAAGGATCCAGAATATCTCAGTGTGCATGAGAAGGCTGTGACAGCGACTCCTACTCTTTTGCAGCAGGCGGCAGTCATCGTTTCACTTGACAAAAAATTAGACGTGTTGTGGAGCTTCATAAAGACACATCTAAATTATAGAATTCTAGTGTTTCTATCAACCCGCAAACAGGTAAAGTTTGTCTATGAAGCATTTAAAAAGCTCCGTCCTGGAATACCCTTGAAGTGTCTTCATGGTAAGATGAAGCAAGAGGGACGAATGGGAACGTACTCACAGTTTTCTGAAAAACGTTGTGTTCTCTTCTCAACTGATGTGGCTTCAAGAGGCCTTGATTTCAACAAGGCAGTTGACTGGGTTGTTCAGGTGGACTGCCCAGACGATGTTGCATCTTACATACACAGAGTTGGACGAACTGCTCGTTATCAATCTAAAGGGCAATCACTTTTATTTCTGATGCCTTCAGAAGTAAAAATGCTTGAGAAATTGAAGGAGGCAAAAATACCTATTAAGTCCACCCAGGCTAACCCGGATAAACTGCAACCAGTGTCTGGGTTGTTAGCATCTTTGCTAGTCAAGTACCCAGATCCACTTCAGGGTCTAGCTAAAAGGGCATTTATCACGTACGTGAAATCTATTCAAAAGCAGAAGGACAAAGAGATTTTTGATGTTACGAATTTACCAGTTGAAGAATTTTCAGCATCACTGGGTCTACCAATGACTCCTAAAATTCGTTTTGCAAACCAGAAAATCAAGTCGAAGAAAGTGCCTGAATTATTTCCCCTTGTTGAACCAGAAAGTTGGGACAAGGAGGATAGGTTGGAGCTTCCAAAAGAGGAGCTCGATATTGGTGATTTTATGGAAGAGGACGGGGAGGAAGATGCTCTTGTAAGAAATGCTACTGCAAATGAGGCAGAGGGGGAAGGACGAAAAATCGAAGATATTATACCGGCTACTCGGGTTTCGAAGAAAAAGAAGTTAAAGATCAATATCCATAGGCCAGTGGGGACTAGAGTCGTCTTTGATGAGGAAGGCAACACGCTACCTCCATTAGCAAGGCTGGCTGATATCAATACCAGTGGTCTGCCTGATGAAATTCATGACATGAAAAATGACTATTACAAGAAGCTTAGGGAAGATATGAAGGTGGTGGACAAGGAAGACAAGGTTCTAGACCGCCAGCGTCGGCGTGAGAAACGTTTGAAGCATAAGATGAAGTTGAAGAGAGGAAACACAGAAGAAGATTCGGAGGATAATCTTTCTGATTCAGAAGGAGAACCAGCTGAGGATCGACCCCGCAAAAAGTCGAAGATATACTTTGGTAGTGATGATGAAGGTGAGAGAGAAGAAAGTAAGAAGAAACTGGGTTTCACTGCTGACTCAATGTCTTTAGAGGAACAAGAAGCTTTGGCTCTCAAATTATTAAATTCCATGCACTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

745

Amino Acids

84.18

Weight (kDa)

8.74

Isoelectric Point (pI)

44.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	90 - 260	6e-44	DEAD/DEAH box helicase
ResIII	PF04851	109 - 252	6.6e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	297 - 406	1.2e-23	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	464 - 508	1.8e-12	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002471)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54910
fragaria_vesca	FvH4_4g36230 FvH4_4g36230 FvH4_4g36230 FvH4_5g34980
malus_domestica	MD08G1022200.v1.1 MD13G1010800.v1.1 MD15G1026900.v1.1
prunus_persica	Prupe.1G342200_v2.0.a1
pyrus_communis	pycom08g13590 pycom13g00910 pycom15g02270 pycom15g02280 pycom15g02290
rosa_chinensis	RchiOBHm_Chr1g0336991 RchiOBHm_Chr4g0445981
rosa_laevigata	RLG00000005702 RLG00000029316
rosa_multiflora	Rmu_co8191210.1_g000001 Rmu_sc0000115.1_g000004 Rmu_sc0003001.1_g000004 Rmu_sc0008543.1_g000002 Rmu_sc0009742.1_g000002 Rmu_sc0036469.1_g000003
rosa_roxburghii	Rroxscaffold_4G00315110 Rroxscaffold_5G00386390
rosa_rugosa	Rorug01G0129500 Rorug01G0130100 Rorug04G0366700 Rorug04G0366700
rosa_samantha	Rh1AG150500 Rh1BG118700 Rh1BG118900 Rh1CG141800 Rh1DG157800 Rh4AG428100 Rh4BG437700 Rh4CG454400 Rh4DG436200
rosa_wichuraiana	Rw1G012290 Rw4G036610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	149
AccB1I	GGYRCC	3 cut(s)	194, 323, 489
AccB7I	CCANNNNNTGG	1 cut(s)	1343
AccI	GTMKAC	1 cut(s)	1504
AciI	CCGC	9 cut(s)	28, 129, 192, 327, 368, 863, 958, 1969, 2089
AclI	AACGTT	3 cut(s)	737, 1084, 1990
AclWI	GGATC	4 cut(s)	794, 807, 1373, 2088
AcoI	YGGCCR	1 cut(s)	154
AcsI	RAATTY	8 cut(s)	73, 619, 936, 1468, 1484, 1521, 1887, 2218
AcuI	CTGAAG	4 cut(s)	581, 623, 1239, 1370
AcyI	GRCGYC	3 cut(s)	144, 237, 324
AfaI	GTAC	4 cut(s)	257, 1064, 1373, 1418
AfiI	CCNNNNNNNGG	8 cut(s)	104, 188, 336, 410, 658, 964, 1343, 1388
AflIII	ACRYGT	1 cut(s)	897
AhdI	GACNNNNNGTC	1 cut(s)	2176
AjiI	CACGTC	1 cut(s)	898
AjnI	CCWGG	4 cut(s)	54, 583, 1003, 1311
AleI	CACNNNNGTG	1 cut(s)	1418
Alw21I	GWGCWC	4 cut(s)	26, 54, 552, 1635
Alw26I	GTCTC	2 cut(s)	126, 223
AlwI	GGATC	4 cut(s)	794, 807, 1373, 2088
AlwNI	CAGNNNCTG	1 cut(s)	1343
Ama87I	CYCGRG	1 cut(s)	1749
AoxI	GGCC	3 cut(s)	154, 1120, 1790
ApeKI	GCWGC	4 cut(s)	208, 211, 420, 856
ApoI	RAATTY	8 cut(s)	73, 619, 936, 1468, 1484, 1521, 1887, 2218
ArsI	GACNNNNNNTTYG	2 cut(s)	446, 478
Asp700I	GAANNNNTTC	1 cut(s)	1569
AspLEI	GCGC	3 cut(s)	246, 301, 326
AspS9I	GGNCC	3 cut(s)	40, 401, 649
AsuC2I	CCSGG	3 cut(s)	162, 337, 1322
AsuHPI	GGTGA	3 cut(s)	176, 1655, 2141
AsuII	TTCGAA	1 cut(s)	1757
AvaI	CYCGRG	1 cut(s)	1749
AvaII	GGWCC	3 cut(s)	40, 401, 649
AxyI	CCTNAGG	1 cut(s)	405
BamHI	GGATCC	1 cut(s)	799
BanI	GGYRCC	3 cut(s)	194, 323, 489
BanII	GRGCYC	2 cut(s)	54, 1635
BarI	GAAGNNNNNNTAC	2 cut(s)	1722, 1754
BbsI	GAAGAC	4 cut(s)	233, 503, 1016, 1959
Bbv12I	GWGCWC	4 cut(s)	26, 54, 552, 1635
BbvCI	CCTCAGC	1 cut(s)	2076
BbvI	GCAGC	4 cut(s)	198, 220, 432, 868
BccI	CCATC	2 cut(s)	390, 404
BceAI	ACGGC	2 cut(s)	141, 605
BcgI	CGANNNNNNTGC	4 cut(s)	184, 218, 1540, 1574
BciT130I	CCWGG	4 cut(s)	56, 585, 1005, 1313
BcnI	CCSGG	3 cut(s)	162, 337, 1322
BcoDI	GTCTC	2 cut(s)	126, 223
BfaI	CTAG	5 cut(s)	941, 1364, 1394, 1803, 1964
BfoI	RGCGCY	1 cut(s)	327
BglII	AGATCT	1 cut(s)	775
BisI	GCNGC	7 cut(s)	193, 209, 212, 327, 421, 857, 864
BlsI	GCNGC	7 cut(s)	194, 210, 213, 328, 422, 858, 865
Bme1390I	CCNGG	7 cut(s)	56, 162, 337, 585, 1005, 1313, 1322
Bme18I	GGWCC	3 cut(s)	40, 401, 649
BmeRI	GACNNNNNGTC	1 cut(s)	2176
BmeT110I	CYCGRG	1 cut(s)	1749
BmgBI	CACGTC	1 cut(s)	898
BmgT120I	GGNCC	3 cut(s)	40, 401, 649
BmiI	GGNNCC	6 cut(s)	109, 196, 325, 402, 491, 801
BmrFI	CCNGG	7 cut(s)	56, 162, 337, 585, 1005, 1313, 1322
BmrI	ACTGGG	3 cut(s)	1156, 1508, 2165
BmsI	GCATC	6 cut(s)	432, 1190, 1239, 1364, 1502, 1663
BmuI	ACTGGG	3 cut(s)	1156, 1508, 2165
BoxI	GACNNNNGTC	1 cut(s)	1805
BpiI	GAAGAC	4 cut(s)	233, 503, 1016, 1959
BplI	GAGNNNNNCTC	2 cut(s)	303, 335
BpmI	CTGGAG	2 cut(s)	38, 567
Bpu10I	CCTNAGC	2 cut(s)	1922, 2076
Bpu14I	TTCGAA	1 cut(s)	1757
BpuEI	CTTGAG	1 cut(s)	1292
BpuMI	CCSGG	3 cut(s)	162, 337, 1322
BsaAI	YACGTR	3 cut(s)	553, 1416, 1420
BsaBI	GATNNNNATC	1 cut(s)	1781
BsaHI	GRCGYC	3 cut(s)	144, 237, 324
BsaJI	CCNNGG	1 cut(s)	1311
BsaXI	ACNNNNNCTCC	6 cut(s)	826, 856, 1595, 1625, 1822, 1852
Bsc4I	CCNNNNNNNGG	8 cut(s)	104, 188, 336, 410, 658, 964, 1343, 1388
Bse118I	RCCGGY	2 cut(s)	188, 1741
Bse1I	ACTGG	9 cut(s)	247, 576, 1151, 1337, 1475, 1503, 1793, 1869, 2160
Bse21I	CCTNAGG	1 cut(s)	405
Bse8I	GATNNNNATC	1 cut(s)	1781
BseBI	CCWGG	4 cut(s)	56, 585, 1005, 1313
BseDI	CCNNGG	1 cut(s)	1311
BseGI	GGATG	2 cut(s)	415, 613
BseJI	GATNNNNATC	1 cut(s)	1781
BseLI	CCNNNNNNNGG	8 cut(s)	104, 188, 336, 410, 658, 964, 1343, 1388
BseMII	CTCAG	5 cut(s)	419, 762, 802, 826, 2067
BseNI	ACTGG	9 cut(s)	247, 576, 1151, 1337, 1475, 1503, 1793, 1869, 2160
BseRI	GAGGAG	1 cut(s)	1643
BseX3I	CGGCCG	1 cut(s)	154
BseXI	GCAGC	4 cut(s)	198, 220, 432, 868
Bsh1285I	CGRYCG	1 cut(s)	157
BshFI	GGCC	3 cut(s)	156, 1122, 1792
BshNI	GGYRCC	3 cut(s)	194, 323, 489
BsiEI	CGRYCG	1 cut(s)	157
BsiHKAI	GWGCWC	4 cut(s)	26, 54, 552, 1635
BsiHKCI	CYCGRG	1 cut(s)	1749
BsiSI	CCGG	5 cut(s)	162, 189, 336, 1322, 1742
BsiWI	CGTACG	1 cut(s)	1416
BslFI	GGGAC	5 cut(s)	144, 414, 1062, 1610, 1813
BslI	CCNNNNNNNGG	8 cut(s)	104, 188, 336, 410, 658, 964, 1343, 1388
BsmAI	GTCTC	2 cut(s)	126, 223
BsmBI	CGTCTC	1 cut(s)	223
BsmFI	GGGAC	5 cut(s)	144, 414, 1062, 1610, 1813
BsmI	GAATGC	1 cut(s)	706
BsnI	GGCC	3 cut(s)	156, 1122, 1792
BsoBI	CYCGRG	1 cut(s)	1749
Bsp119I	TTCGAA	1 cut(s)	1757
Bsp1286I	GDGCHC	4 cut(s)	26, 54, 552, 1635
Bsp143I	GATC	6 cut(s)	664, 775, 799, 1378, 1776, 2080
BspACI	CCGC	9 cut(s)	28, 129, 192, 327, 368, 863, 958, 1969, 2089
BspANI	GGCC	3 cut(s)	156, 1122, 1792
BspCNI	CTCAG	5 cut(s)	418, 761, 801, 825, 2068
BspHI	TCATGA	1 cut(s)	1891
BspLI	GGNNCC	6 cut(s)	109, 196, 325, 402, 491, 801
BspPI	GGATC	4 cut(s)	794, 807, 1373, 2088
BspT104I	TTCGAA	1 cut(s)	1757
BspT107I	GGYRCC	3 cut(s)	194, 323, 489
BsrFI	RCCGGY	2 cut(s)	188, 1741
BsrI	ACTGG	9 cut(s)	247, 576, 1151, 1337, 1475, 1503, 1793, 1869, 2160
BssAI	RCCGGY	2 cut(s)	188, 1741
BssECI	CCNNGG	1 cut(s)	1311
BssMI	GATC	6 cut(s)	664, 775, 799, 1378, 1776, 2080
BssNI	GRCGYC	3 cut(s)	144, 237, 324
Bst2UI	CCWGG	4 cut(s)	56, 585, 1005, 1313
Bst4CI	ACNGT	1 cut(s)	1071
Bst6I	CTCTTC	5 cut(s)	534, 749, 1100, 1650, 2009
BstACI	GRCGYC	3 cut(s)	144, 237, 324
BstBAI	YACGTR	3 cut(s)	553, 1416, 1420
BstBI	TTCGAA	1 cut(s)	1757
BstC8I	GCNNGC	5 cut(s)	32, 190, 861, 1854, 1973
BstDEI	CTNAG	7 cut(s)	222, 405, 748, 788, 812, 1922, 2076
BstENI	CCTNNNNNAGG	1 cut(s)	656
BstF5I	GGATG	2 cut(s)	415, 613
BstH2I	RGCGCY	1 cut(s)	327
BstHHI	GCGC	3 cut(s)	246, 301, 326
BstKTI	GATC	6 cut(s)	667, 778, 802, 1381, 1779, 2083
BstMAI	GTCTC	2 cut(s)	126, 223
BstMBI	GATC	6 cut(s)	664, 775, 799, 1378, 1776, 2080
BstMCI	CGRYCG	1 cut(s)	157
BstMWI	GCNNNNNNNGC	6 cut(s)	298, 598, 836, 1403, 1703, 1853
BstNI	CCWGG	4 cut(s)	56, 585, 1005, 1313
BstPAI	GACNNNNGTC	1 cut(s)	1805
BstSCI	CCNGG	7 cut(s)	54, 160, 335, 583, 1003, 1311, 1320
BstV1I	GCAGC	4 cut(s)	198, 220, 432, 868
BstV2I	GAAGAC	4 cut(s)	233, 503, 1016, 1959
BstX2I	RGATCY	3 cut(s)	775, 799, 1378
BstXI	CCANNNNNNTGG	1 cut(s)	1593
BstYI	RGATCY	3 cut(s)	775, 799, 1378
BstZI	CGGCCG	1 cut(s)	154
Bsu36I	CCTNAGG	1 cut(s)	405
BsuRI	GGCC	3 cut(s)	156, 1122, 1792
BtrI	CACGTC	1 cut(s)	898
BtsCI	GGATG	2 cut(s)	415, 613
BtsI	GCAGTG	1 cut(s)	2163
BtsIMutI	CAGTG	8 cut(s)	254, 695, 820, 1344, 1496, 1800, 1876, 2163
Cac8I	GCNNGC	5 cut(s)	32, 190, 861, 1854, 1973
CaiI	CAGNNNCTG	1 cut(s)	1343
CciI	TCATGA	1 cut(s)	1891
CfoI	GCGC	3 cut(s)	246, 301, 326
Cfr10I	RCCGGY	2 cut(s)	188, 1741
Cfr13I	GGNCC	3 cut(s)	40, 401, 649
CseI	GACGC	3 cut(s)	152, 245, 1964
Csp6I	GTAC	4 cut(s)	256, 1063, 1372, 1417
CspCI	CAANNNNNGTGG	4 cut(s)	1089, 1124, 1852, 1887
CviAII	CATG	9 cut(s)	78, 118, 296, 604, 821, 1028, 1892, 1897, 2224
CviQI	GTAC	4 cut(s)	256, 1063, 1372, 1417
DdeI	CTNAG	7 cut(s)	222, 405, 748, 788, 812, 1922, 2076
DinI	GGCGCC	1 cut(s)	325
DpnI	GATC	6 cut(s)	666, 777, 801, 1380, 1778, 2082
DpnII	GATC	6 cut(s)	664, 775, 799, 1378, 1776, 2080
DraI	TTTAAA	1 cut(s)	991
DrdI	GACNNNNNNGTC	1 cut(s)	149
DriI	GACNNNNNGTC	1 cut(s)	2176
DseDI	GACNNNNNNGTC	1 cut(s)	149
EaeI	YGGCCR	1 cut(s)	154
EagI	CGGCCG	1 cut(s)	154
Eam1104I	CTCTTC	5 cut(s)	534, 749, 1100, 1650, 2009
Eam1105I	GACNNNNNGTC	1 cut(s)	2176
EarI	CTCTTC	5 cut(s)	534, 749, 1100, 1650, 2009
Ecl136II	GAGCTC	2 cut(s)	52, 1633
EclXI	CGGCCG	1 cut(s)	154
Eco147I	AGGCCT	1 cut(s)	1122
Eco24I	GRGCYC	2 cut(s)	54, 1635
Eco32I	GATATC	1 cut(s)	1861
Eco47I	GGWCC	3 cut(s)	40, 401, 649
Eco52I	CGGCCG	1 cut(s)	154
Eco53kI	GAGCTC	2 cut(s)	52, 1633
Eco57I	CTGAAG	4 cut(s)	581, 623, 1239, 1370
Eco81I	CCTNAGG	1 cut(s)	405
Eco88I	CYCGRG	1 cut(s)	1749
EcoICRI	GAGCTC	2 cut(s)	52, 1633
EcoNI	CCTNNNNNAGG	1 cut(s)	656
EcoO109I	RGGNCCY	2 cut(s)	40, 401
EcoRI	GAATTC	2 cut(s)	73, 936
EcoRII	CCWGG	4 cut(s)	54, 583, 1003, 1311
EcoRV	GATATC	1 cut(s)	1861
EcoT38I	GRGCYC	2 cut(s)	54, 1635
EgeI	GGCGCC	1 cut(s)	325
EheI	GGCGCC	1 cut(s)	325
Esp3I	CGTCTC	1 cut(s)	223
FaeI	CATG	9 cut(s)	81, 121, 299, 607, 824, 1031, 1895, 1900, 2227
FalI	AAGNNNNNCTT	4 cut(s)	1662, 1694, 2093, 2125
FaqI	GGGAC	5 cut(s)	144, 414, 1062, 1610, 1813
FatI	CATG	9 cut(s)	77, 117, 295, 603, 820, 1027, 1891, 1896, 2223
FauI	CCCGC	4 cut(s)	35, 136, 965, 2096
FblI	GTMKAC	1 cut(s)	1504
Fnu4HI	GCNGC	7 cut(s)	193, 209, 212, 327, 421, 857, 864
FokI	GGATG	2 cut(s)	422, 620
FriOI	GRGCYC	2 cut(s)	54, 1635
Fsp4HI	GCNGC	7 cut(s)	193, 209, 212, 327, 421, 857, 864
FspBI	CTAG	5 cut(s)	941, 1364, 1394, 1803, 1964
GlaI	GCGC	3 cut(s)	245, 300, 325
GluI	GCNGC	7 cut(s)	193, 209, 212, 327, 421, 857, 864
GsuI	CTGGAG	2 cut(s)	38, 567
HaeII	RGCGCY	1 cut(s)	327
HaeIII	GGCC	3 cut(s)	156, 1122, 1792
HapII	CCGG	5 cut(s)	162, 189, 336, 1322, 1742
HgaI	GACGC	3 cut(s)	152, 245, 1964
HhaI	GCGC	3 cut(s)	246, 301, 326
Hin1I	GRCGYC	3 cut(s)	144, 237, 324
Hin1II	CATG	9 cut(s)	81, 121, 299, 607, 824, 1031, 1895, 1900, 2227
Hin6I	GCGC	3 cut(s)	244, 299, 324
HinP1I	GCGC	3 cut(s)	244, 299, 324
HincII	GTYRAC	1 cut(s)	1144
HindII	GTYRAC	1 cut(s)	1144
HindIII	AAGCTT	2 cut(s)	1919, 2196
HinfI	GANTC	9 cut(s)	86, 201, 786, 841, 1513, 1806, 2036, 2057, 2171
HpaII	CCGG	5 cut(s)	162, 189, 336, 1322, 1742
HphI	GGTGA	3 cut(s)	176, 1655, 2141
Hpy166II	GTNNAC	5 cut(s)	1144, 1162, 1308, 1505, 1945
Hpy188III	TCNNGA	7 cut(s)	342, 407, 674, 803, 1116, 1892, 1964
Hpy8I	GTNNAC	5 cut(s)	1144, 1162, 1308, 1505, 1945
Hpy99I	CGWCG	3 cut(s)	146, 155, 1980
HpyCH4III	ACNGT	1 cut(s)	1071
HpyCH4IV	ACGT	9 cut(s)	258, 552, 737, 897, 1061, 1084, 1415, 1419, 1990
HpyF10VI	GCNNNNNNNGC	6 cut(s)	298, 598, 836, 1403, 1703, 1853
HpyF3I	CTNAG	7 cut(s)	222, 405, 748, 788, 812, 1922, 2076
HpySE526I	ACGT	9 cut(s)	258, 552, 737, 897, 1061, 1084, 1415, 1419, 1990
Hsp92I	GRCGYC	3 cut(s)	144, 237, 324
Hsp92II	CATG	9 cut(s)	81, 121, 299, 607, 824, 1031, 1895, 1900, 2227
HspAI	GCGC	3 cut(s)	244, 299, 324
KasI	GGCGCC	1 cut(s)	323
KroI	GCCGGC	1 cut(s)	188
KroNI	GCCGGC	1 cut(s)	190
Kzo9I	GATC	6 cut(s)	664, 775, 799, 1378, 1776, 2080
LmnI	GCTCC	9 cut(s)	29, 49, 57, 113, 547, 906, 1002, 1615, 1630
Lsp1109I	GCAGC	4 cut(s)	198, 220, 432, 868
LweI	GCATC	6 cut(s)	432, 1190, 1239, 1364, 1502, 1663
MaeI	CTAG	5 cut(s)	941, 1364, 1394, 1803, 1964
MaeII	ACGT	9 cut(s)	258, 552, 737, 897, 1061, 1084, 1415, 1419, 1990
MaeIII	GTNAC	3 cut(s)	164, 832, 1462
MalI	GATC	6 cut(s)	666, 777, 801, 1380, 1778, 2082
MboI	GATC	6 cut(s)	664, 775, 799, 1378, 1776, 2080
MflI	RGATCY	3 cut(s)	775, 799, 1378
MhlI	GDGCHC	4 cut(s)	26, 54, 552, 1635
Mly113I	GGCGCC	1 cut(s)	324
MlyI	GAGTC	5 cut(s)	780, 835, 1507, 1815, 2165
MmeI	TCCRAC	6 cut(s)	135, 165, 461, 661, 1180, 1593
MreI	CGCCGGCG	1 cut(s)	188
MroNI	GCCGGC	1 cut(s)	188
MroXI	GAANNNNTTC	1 cut(s)	1569
MseI	TTAA	7 cut(s)	473, 687, 771, 990, 1302, 1772, 2216
MslI	CAYNNNNRTG	3 cut(s)	555, 606, 1418
MspA1I	CMGCKG	2 cut(s)	211, 2075
MspI	CCGG	5 cut(s)	162, 189, 336, 1322, 1742
MspR9I	CCNGG	7 cut(s)	56, 162, 337, 585, 1005, 1313, 1322
Mva1269I	GAATGC	1 cut(s)	706
MvaI	CCWGG	4 cut(s)	56, 585, 1005, 1313
MwoI	GCNNNNNNNGC	6 cut(s)	298, 598, 836, 1403, 1703, 1853
NaeI	GCCGGC	1 cut(s)	190
NarI	GGCGCC	1 cut(s)	324
NciI	CCSGG	3 cut(s)	162, 337, 1322
NdeII	GATC	6 cut(s)	664, 775, 799, 1378, 1776, 2080
NgoMIV	GCCGGC	1 cut(s)	188
NlaIII	CATG	9 cut(s)	81, 121, 299, 607, 824, 1031, 1895, 1900, 2227
NlaIV	GGNNCC	6 cut(s)	109, 196, 325, 402, 491, 801
NmeAIII	GCCGAG	1 cut(s)	300
NmuCI	GTSAC	2 cut(s)	164, 832
NspV	TTCGAA	1 cut(s)	1757
OliI	CACNNNNGTG	1 cut(s)	1418
PagI	TCATGA	1 cut(s)	1891
PceI	AGGCCT	1 cut(s)	1122
PcsI	WCGNNNNNNNCGW	1 cut(s)	1210
PctI	GAATGC	1 cut(s)	706
PdiI	GCCGGC	1 cut(s)	190
PdmI	GAANNNNTTC	1 cut(s)	1569
PfeI	GAWTC	4 cut(s)	86, 201, 2036, 2057
Pfl23II	CGTACG	1 cut(s)	1416
PflFI	GACNNNGTC	1 cut(s)	337
PflMI	CCANNNNNTGG	1 cut(s)	1343
PfoI	TCCNGGA	3 cut(s)	54, 583, 1003
PkrI	GCNGC	7 cut(s)	194, 210, 213, 328, 422, 858, 865
PleI	GAGTC	5 cut(s)	780, 835, 1507, 1814, 2165
PluTI	GGCGCC	1 cut(s)	327
PpsI	GAGTC	5 cut(s)	780, 835, 1507, 1814, 2165
Ppu21I	YACGTR	3 cut(s)	553, 1416, 1420
PpuMI	RGGWCCY	2 cut(s)	40, 401
PshAI	GACNNNNGTC	1 cut(s)	1805
Psp124BI	GAGCTC	2 cut(s)	54, 1635
Psp1406I	AACGTT	3 cut(s)	737, 1084, 1990
Psp5II	RGGWCCY	2 cut(s)	40, 401
Psp6I	CCWGG	4 cut(s)	54, 583, 1003, 1311
PspGI	CCWGG	4 cut(s)	54, 583, 1003, 1311
PspLI	CGTACG	1 cut(s)	1416
PspN4I	GGNNCC	6 cut(s)	109, 196, 325, 402, 491, 801
PspPI	GGNCC	3 cut(s)	40, 401, 649
PspPPI	RGGWCCY	2 cut(s)	40, 401
PstNI	CAGNNNCTG	1 cut(s)	1343
PsuI	RGATCY	3 cut(s)	775, 799, 1378
PsyI	GACNNNGTC	1 cut(s)	337
PvuII	CAGCTG	2 cut(s)	211, 2075
RsaI	GTAC	4 cut(s)	257, 1064, 1373, 1418
RsaNI	GTAC	4 cut(s)	256, 1063, 1372, 1417
RseI	CAYNNNNRTG	3 cut(s)	555, 606, 1418
SacI	GAGCTC	2 cut(s)	54, 1635
SaqAI	TTAA	7 cut(s)	473, 687, 771, 990, 1302, 1772, 2216
SatI	GCNGC	7 cut(s)	193, 209, 212, 327, 421, 857, 864
Sau3AI	GATC	6 cut(s)	664, 775, 799, 1378, 1776, 2080
Sau96I	GGNCC	3 cut(s)	40, 401, 649
SchI	GAGTC	5 cut(s)	780, 835, 1507, 1815, 2165
ScrFI	CCNGG	7 cut(s)	56, 162, 337, 585, 1005, 1313, 1322
SduI	GDGCHC	4 cut(s)	26, 54, 552, 1635
SfaNI	GCATC	6 cut(s)	432, 1190, 1239, 1364, 1502, 1663
SfoI	GGCGCC	1 cut(s)	325
SfuI	TTCGAA	1 cut(s)	1757
SgrAI	CRCCGGYG	1 cut(s)	188
SinI	GGWCC	3 cut(s)	40, 401, 649
SmiMI	CAYNNNNRTG	3 cut(s)	555, 606, 1418
SmlI	CTYRAG	1 cut(s)	1271
SmoI	CTYRAG	1 cut(s)	1271
SseBI	AGGCCT	1 cut(s)	1122
SsiI	CCGC	9 cut(s)	28, 129, 192, 327, 368, 863, 958, 1969, 2089
SspDI	GGCGCC	1 cut(s)	323
SspMI	CTAG	5 cut(s)	941, 1364, 1394, 1803, 1964
SstI	GAGCTC	2 cut(s)	54, 1635
StuI	AGGCCT	1 cut(s)	1122
StyD4I	CCNGG	7 cut(s)	54, 160, 335, 583, 1003, 1311, 1320
TaaI	ACNGT	1 cut(s)	1071
TaiI	ACGT	9 cut(s)	261, 555, 740, 900, 1064, 1087, 1418, 1422, 1993
TaqI	TCGA	9 cut(s)	17, 63, 204, 1550, 1635, 1728, 1757, 2083, 2099
TauI	GCSGC	3 cut(s)	195, 329, 866
TfiI	GAWTC	4 cut(s)	86, 201, 2036, 2057
Tru1I	TTAA	7 cut(s)	473, 687, 771, 990, 1302, 1772, 2216
Tru9I	TTAA	7 cut(s)	473, 687, 771, 990, 1302, 1772, 2216
TscAI	CASTG	8 cut(s)	254, 702, 820, 1344, 1503, 1800, 1876, 2170
TseFI	GTSAC	2 cut(s)	164, 832
TseI	GCWGC	4 cut(s)	208, 211, 420, 856
Tsp45I	GTSAC	2 cut(s)	164, 832
TspGWI	ACGGA	1 cut(s)	989
TspRI	CASTG	8 cut(s)	254, 702, 820, 1344, 1503, 1800, 1876, 2170
Tth111I	GACNNNGTC	1 cut(s)	337
Van91I	CCANNNNNTGG	1 cut(s)	1343
VpaK11BI	GGWCC	3 cut(s)	40, 401, 649
XagI	CCTNNNNNAGG	1 cut(s)	656
XapI	RAATTY	8 cut(s)	73, 619, 936, 1468, 1484, 1521, 1887, 2218
XbaI	TCTAGA	1 cut(s)	1963
XcmI	CCANNNNNNNNNTGG	1 cut(s)	302
XmiI	GTMKAC	1 cut(s)	1504
XmnI	GAANNNNTTC	1 cut(s)	1569
XspI	CTAG	5 cut(s)	941, 1364, 1394, 1803, 1964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.