Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0002471

Physical Location & Seq

Genomic Coordinates Forward (+)

68703644 .. 68708288

Gene Structure 4645 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG454400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

ATGAGACACCAAATTTTGAGTGTTCACAGCTACAGGGAACAGGTTTTGGTCCTCGATGAGGCAGATCGTATTCTTGATGTGGGATTTAAGAAGGCACTGAACGCCATCATTTCACAGCTACCTAAGAGAAGACAAACCTTTCTCTTCTCTGCAACTCAAACAAAGTCGGTTCAAGATCTTGTAAGACTTAGTTTGAAGGACCCAGAGTACCTTAGCGTGCATGAGAAAGCTACAAGAGCCACTCCTAATCGTTTGAAGCAAACTGCAATGATTGTTCCTCTTGATAAAAAACTAGATATGCTGTGGAGCTTCATAAAGGCGCATCTGAATTCTAGGATTTTAGTGTTCTTATCGACACGTAAACAGGTAAAGTTTGTCTATGAAGCATTTAAGAAGCTCCGACCTGGAATACCCCTGAAGTGTCTTCATGGTAAGATGAAGCAAGAGGGAAGAATGGGAACATACTCGCAGTTTCATGAAAAAGCTACGGTCCTTTTCTCGACTGATGTAGCTTCGAGAGGCCTTGATTTCAACCAGGCAGTCGACTGGGTTGTTCAGGTGGATTGTCCAGAAGATGTTGCATCCTATATACACAGAGTTGGCCGAACTGCGCGCTATCAATCAGAAGGGCGATCACTTTTATTTGTGATACCCTCAGAAACAAAAATGCTTGAGAAATTAGATGCCGCAAAAATACCCATAGAAAGCATCAAGGCTAACAACAAGAGACTGCAACCTGTCAGTGGGTTGCTATCAGCTTTGCTAGTCAAGTACCCAGACACACTTCCAGGTCTGGCCAAAAGGGCCTTTATCACGTACTTGAAGTCTATTCAAAATCACAAGGACAAAGAAGTTTTTGACGTAATGCAACTGCCAGTTGATGAGTTTTCAGCATCAATGGGTTTACCAATGACCCCCAAGATCCGTTTTTCAAACCACAAAATGAAGTCCAAGAAACCATTGGAATTGTCTCCCATTGTTGAACCAGAAAGTTCCGACATGGAGAATGCATTAGAGACTCCCAGAGAGGAGCTTGATATTGGTGATTTGGAGGAAGAGGAAGTGGAGAAAGGTCTTCTTCGAATAAATGATGCACCAACTGAGAGAGAGGAGGAAGGAAGACAGATTGCAGATATTATGCCGGCAACTCGGGTTCTGAAGAAAAAGAAGTTGAAGATCAATCTCGATAGACCATTGGGAACTAGGAAGGTCTATGATGAGGACGGCAATTTGGTGGACCCACTTGCAAGGCTGGCTGGGCCCAGTGATCCTAGCGTTCTTGATGGACTTCAAGACAAGAAAGAAGAATTTCATAAGAGGCTTAGGGAAGAGGTGAAGATGGTAGACAAGGAAGATAAGGTAAAAGATCGCCAGCGTCGCCGTGAAAAACGAATGAAGGAGAAGATGAAGTTGAAGAGAGCAGATGCAGAAGAAGAGGAGGATGATGATTCTGAGTCGGAAGAAGAGCCATCTGATAATCGATCGCACAAAAGGTCAAAGATTTACTTTGATAGTGATGATAATGGTGAGAGAGAAGAAAAGAAGGATAAAGCTGGGTTCATTGCTGACCCAAGTTATATAGAGCATCAGCAAGCTTTGGCTCTCAAACTGTTAGATTCTATGCACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

543

Amino Acids

62.48

Weight (kDa)

8.7

Isoelectric Point (pI)

50.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	14 - 60	1.1e-09	DEAD/DEAH box helicase
Helicase_C	PF00271	97 - 206	5.4e-24	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	265 - 308	1e-10	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002471)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54910
fragaria_vesca	FvH4_4g36230 FvH4_4g36230 FvH4_4g36230 FvH4_5g34980
malus_domestica	MD08G1022200.v1.1 MD13G1010800.v1.1 MD15G1026900.v1.1
prunus_persica	Prupe.1G342200_v2.0.a1
pyrus_communis	pycom08g13590 pycom13g00910 pycom15g02270 pycom15g02280 pycom15g02290
rosa_chinensis	RchiOBHm_Chr1g0336991 RchiOBHm_Chr4g0445981
rosa_laevigata	RLG00000005702 RLG00000029316
rosa_multiflora	Rmu_co8191210.1_g000001 Rmu_sc0000115.1_g000004 Rmu_sc0003001.1_g000004 Rmu_sc0008543.1_g000002 Rmu_sc0009742.1_g000002 Rmu_sc0036469.1_g000003
rosa_roxburghii	Rroxscaffold_4G00315110 Rroxscaffold_5G00386390
rosa_rugosa	Rorug01G0129500 Rorug01G0130100 Rorug04G0366700 Rorug04G0366700
rosa_samantha	Rh1AG150500 Rh1BG118700 Rh1BG118900 Rh1CG141800 Rh1DG157800 Rh4AG428100 Rh4BG437700 Rh4CG454400 Rh4DG436200
rosa_wichuraiana	Rw1G012290 Rw4G036610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	543, 1344
AccII	CGCG	1 cut(s)	613
AciI	CCGC	1 cut(s)	687
AclWI	GGATC	2 cut(s)	916, 1262
AcoI	YGGCCR	2 cut(s)	601, 795
AcsI	RAATTY	3 cut(s)	12, 328, 1307
AcuI	CTGAAG	2 cut(s)	437, 1178
AfaI	GTAC	3 cut(s)	209, 773, 818
AfiI	CCNNNNNNNGG	2 cut(s)	58, 743
AflIII	ACRYGT	1 cut(s)	356
AjnI	CCWGG	3 cut(s)	403, 534, 787
Alw26I	GTCTC	3 cut(s)	721, 975, 1010
AlwI	GGATC	2 cut(s)	916, 1262
Ama87I	CYCGRG	1 cut(s)	1149
AoxI	GGCC	5 cut(s)	520, 601, 795, 804, 1259
ApaI	GGGCCC	1 cut(s)	1263
ApoI	RAATTY	3 cut(s)	12, 328, 1307
Asp700I	GAANNNNTTC	1 cut(s)	1308
AspLEI	GCGC	3 cut(s)	322, 613, 615
AspS9I	GGNCC	7 cut(s)	49, 199, 490, 804, 1237, 1259, 1260
AsuHPI	GGTGA	3 cut(s)	1055, 1345, 1538
AsuII	TTCGAA	1 cut(s)	1081
AvaI	CYCGRG	1 cut(s)	1149
AvaII	GGWCC	4 cut(s)	49, 199, 490, 1237
BaeGI	GKGCMC	1 cut(s)	1263
BalI	TGGCCA	1 cut(s)	797
BanII	GRGCYC	1 cut(s)	1263
BbsI	GAAGAC	4 cut(s)	136, 416, 1067, 1126
BccI	CCATC	4 cut(s)	113, 1277, 1333, 1477
BceAI	ACGGC	2 cut(s)	1240, 1365
BciT130I	CCWGG	3 cut(s)	405, 536, 789
BcoDI	GTCTC	3 cut(s)	721, 975, 1010
BfaI	CTAG	5 cut(s)	293, 333, 764, 1203, 1272
BfmI	CTRYAG	1 cut(s)	31
BglI	GCCNNNNNGGC	1 cut(s)	803
BglII	AGATCT	1 cut(s)	175
BisI	GCNGC	1 cut(s)	687
BlsI	GCNGC	1 cut(s)	688
Bme1390I	CCNGG	3 cut(s)	405, 536, 789
Bme18I	GGWCC	4 cut(s)	49, 199, 490, 1237
BmeT110I	CYCGRG	1 cut(s)	1149
BmgT120I	GGNCC	7 cut(s)	49, 199, 490, 804, 1237, 1259, 1260
BmiI	GGNNCC	3 cut(s)	201, 1239, 1261
BmrFI	CCNGG	3 cut(s)	405, 536, 789
BmrI	ACTGGG	2 cut(s)	556, 1257
BmsI	GCATC	8 cut(s)	331, 590, 673, 717, 902, 1081, 1414, 1594
BmuI	ACTGGG	2 cut(s)	556, 1257
BpiI	GAAGAC	4 cut(s)	136, 416, 1067, 1126
Bpu10I	CCTNAGC	2 cut(s)	212, 1322
Bpu14I	TTCGAA	1 cut(s)	1081
BpuEI	CTTGAG	1 cut(s)	692
Bsa29I	ATCGAT	1 cut(s)	1480
BsaAI	YACGTR	2 cut(s)	359, 816
Bsc4I	CCNNNNNNNGG	2 cut(s)	58, 743
Bse118I	RCCGGY	1 cut(s)	1141
Bse1I	ACTGG	3 cut(s)	551, 875, 1263
Bse3DI	GCAATG	2 cut(s)	273, 1560
BseBI	CCWGG	3 cut(s)	405, 536, 789
BseCI	ATCGAT	1 cut(s)	1480
BseGI	GGATG	2 cut(s)	581, 1447
BseLI	CCNNNNNNNGG	2 cut(s)	58, 743
BseMI	GCAATG	2 cut(s)	273, 1560
BseMII	CTCAG	3 cut(s)	669, 1092, 1443
BseNI	ACTGG	3 cut(s)	551, 875, 1263
BsePI	GCGCGC	1 cut(s)	611
BseRI	GAGGAG	3 cut(s)	1043, 1124, 1451
BseSI	GKGCMC	1 cut(s)	1263
BseYI	CCCAGC	2 cut(s)	1256, 1553
Bsh1236I	CGCG	1 cut(s)	613
Bsh1285I	CGRYCG	1 cut(s)	1484
BshFI	GGCC	5 cut(s)	522, 603, 797, 806, 1261
BshVI	ATCGAT	1 cut(s)	1480
BsiEI	CGRYCG	1 cut(s)	1484
BsiHKCI	CYCGRG	1 cut(s)	1149
BsiSI	CCGG	1 cut(s)	1142
BslI	CCNNNNNNNGG	2 cut(s)	58, 743
BsmAI	GTCTC	3 cut(s)	721, 975, 1010
BsmI	GAATGC	1 cut(s)	1012
BsnI	GGCC	5 cut(s)	522, 603, 797, 806, 1261
BsoBI	CYCGRG	1 cut(s)	1149
Bsp119I	TTCGAA	1 cut(s)	1081
Bsp120I	GGGCCC	1 cut(s)	1259
Bsp1286I	GDGCHC	1 cut(s)	1263
Bsp143I	GATC	8 cut(s)	64, 175, 632, 921, 1176, 1267, 1366, 1481
BspACI	CCGC	1 cut(s)	687
BspANI	GGCC	5 cut(s)	522, 603, 797, 806, 1261
BspCNI	CTCAG	3 cut(s)	668, 1093, 1444
BspDI	ATCGAT	1 cut(s)	1480
BspFNI	CGCG	1 cut(s)	613
BspHI	TCATGA	1 cut(s)	475
BspLI	GGNNCC	3 cut(s)	201, 1239, 1261
BspPI	GGATC	2 cut(s)	916, 1262
BspQI	GCTCTTC	1 cut(s)	1458
BspT104I	TTCGAA	1 cut(s)	1081
BsrDI	GCAATG	2 cut(s)	273, 1560
BsrFI	RCCGGY	1 cut(s)	1141
BsrI	ACTGG	3 cut(s)	551, 875, 1263
BssAI	RCCGGY	1 cut(s)	1141
BssHII	GCGCGC	1 cut(s)	611
BssMI	GATC	8 cut(s)	64, 175, 632, 921, 1176, 1267, 1366, 1481
Bst2UI	CCWGG	3 cut(s)	405, 536, 789
Bst4CI	ACNGT	2 cut(s)	490, 1611
Bst6I	CTCTTC	6 cut(s)	149, 1050, 1323, 1409, 1428, 1458
BstBAI	YACGTR	2 cut(s)	359, 816
BstBI	TTCGAA	1 cut(s)	1081
BstC8I	GCNNGC	6 cut(s)	218, 613, 1143, 1254, 1373, 1593
BstDEI	CTNAG	7 cut(s)	123, 188, 212, 655, 1101, 1322, 1452
BstENI	CCTNNNNNAGG	1 cut(s)	56
BstF5I	GGATG	2 cut(s)	581, 1447
BstFNI	CGCG	1 cut(s)	613
BstHHI	GCGC	3 cut(s)	322, 613, 615
BstKTI	GATC	8 cut(s)	67, 178, 635, 924, 1179, 1270, 1369, 1484
BstMAI	GTCTC	3 cut(s)	721, 975, 1010
BstMBI	GATC	8 cut(s)	64, 175, 632, 921, 1176, 1267, 1366, 1481
BstMCI	CGRYCG	1 cut(s)	1484
BstMWI	GCNNNNNNNGC	6 cut(s)	101, 236, 803, 1253, 1258, 1377
BstNI	CCWGG	3 cut(s)	405, 536, 789
BstSCI	CCNGG	3 cut(s)	403, 534, 787
BstSFI	CTRYAG	1 cut(s)	31
BstSLI	GKGCMC	1 cut(s)	1263
BstUI	CGCG	1 cut(s)	613
BstV2I	GAAGAC	4 cut(s)	136, 416, 1067, 1126
BstX2I	RGATCY	2 cut(s)	175, 921
BstYI	RGATCY	2 cut(s)	175, 921
Bsu15I	ATCGAT	1 cut(s)	1480
BsuRI	GGCC	5 cut(s)	522, 603, 797, 806, 1261
BsuTUI	ATCGAT	1 cut(s)	1480
BtsCI	GGATG	2 cut(s)	581, 1447
BtsIMutI	CAGTG	3 cut(s)	95, 748, 1270
Cac8I	GCNNGC	6 cut(s)	218, 613, 1143, 1254, 1373, 1593
CciI	TCATGA	1 cut(s)	475
CfoI	GCGC	3 cut(s)	322, 613, 615
Cfr10I	RCCGGY	1 cut(s)	1141
Cfr13I	GGNCC	7 cut(s)	49, 199, 490, 804, 1237, 1259, 1260
ClaI	ATCGAT	1 cut(s)	1480
CseI	GACGC	1 cut(s)	1364
Csp6I	GTAC	3 cut(s)	208, 772, 817
CviAII	CATG	4 cut(s)	221, 428, 476, 1000
CviQI	GTAC	3 cut(s)	208, 772, 817
DdeI	CTNAG	7 cut(s)	123, 188, 212, 655, 1101, 1322, 1452
DpnI	GATC	8 cut(s)	66, 177, 634, 923, 1178, 1269, 1368, 1483
DpnII	GATC	8 cut(s)	64, 175, 632, 921, 1176, 1267, 1366, 1481
EaeI	YGGCCR	2 cut(s)	601, 795
Eam1104I	CTCTTC	6 cut(s)	149, 1050, 1323, 1409, 1428, 1458
EarI	CTCTTC	6 cut(s)	149, 1050, 1323, 1409, 1428, 1458
Eco147I	AGGCCT	1 cut(s)	522
Eco24I	GRGCYC	1 cut(s)	1263
Eco47I	GGWCC	4 cut(s)	49, 199, 490, 1237
Eco57I	CTGAAG	2 cut(s)	437, 1178
Eco88I	CYCGRG	1 cut(s)	1149
EcoNI	CCTNNNNNAGG	1 cut(s)	56
EcoO109I	RGGNCCY	2 cut(s)	199, 804
EcoRI	GAATTC	1 cut(s)	328
EcoRII	CCWGG	3 cut(s)	403, 534, 787
EcoT22I	ATGCAT	1 cut(s)	1012
EcoT38I	GRGCYC	1 cut(s)	1263
FaeI	CATG	4 cut(s)	224, 431, 479, 1003
FalI	AAGNNNNNCTT	4 cut(s)	1062, 1094, 1490, 1522
FatI	CATG	4 cut(s)	220, 427, 475, 999
FblI	GTMKAC	2 cut(s)	543, 1344
Fnu4HI	GCNGC	1 cut(s)	687
FokI	GGATG	2 cut(s)	568, 1454
FriOI	GRGCYC	1 cut(s)	1263
Fsp4HI	GCNGC	1 cut(s)	687
FspBI	CTAG	5 cut(s)	293, 333, 764, 1203, 1272
GlaI	GCGC	3 cut(s)	321, 612, 614
GluI	GCNGC	1 cut(s)	687
GsaI	CCCAGC	2 cut(s)	1260, 1557
HaeIII	GGCC	5 cut(s)	522, 603, 797, 806, 1261
HapII	CCGG	1 cut(s)	1142
HgaI	GACGC	1 cut(s)	1364
HhaI	GCGC	3 cut(s)	322, 613, 615
Hin1II	CATG	4 cut(s)	224, 431, 479, 1003
Hin6I	GCGC	3 cut(s)	320, 611, 613
HinP1I	GCGC	3 cut(s)	320, 611, 613
HincII	GTYRAC	1 cut(s)	544
HindII	GTYRAC	1 cut(s)	544
HindIII	AAGCTT	1 cut(s)	1593
HinfI	GANTC	4 cut(s)	1018, 1448, 1454, 1616
HpaII	CCGG	1 cut(s)	1142
HphI	GGTGA	3 cut(s)	1055, 1345, 1538
Hpy166II	GTNNAC	6 cut(s)	25, 362, 544, 905, 1237, 1345
Hpy188I	TCNGA	9 cut(s)	327, 401, 625, 658, 997, 1158, 1453, 1459, 1474
Hpy8I	GTNNAC	6 cut(s)	25, 362, 544, 905, 1237, 1345
Hpy99I	CGWCG	1 cut(s)	1380
HpyAV	CCTTC	7 cut(s)	85, 190, 620, 1109, 1201, 1390, 1537
HpyCH4III	ACNGT	2 cut(s)	490, 1611
HpyCH4IV	ACGT	3 cut(s)	358, 815, 861
HpyF10VI	GCNNNNNNNGC	6 cut(s)	101, 236, 803, 1253, 1258, 1377
HpyF3I	CTNAG	7 cut(s)	123, 188, 212, 655, 1101, 1322, 1452
HpySE526I	ACGT	3 cut(s)	358, 815, 861
Hsp92II	CATG	4 cut(s)	224, 431, 479, 1003
HspAI	GCGC	3 cut(s)	320, 611, 613
KroI	GCCGGC	1 cut(s)	1141
KroNI	GCCGGC	1 cut(s)	1143
Kzo9I	GATC	8 cut(s)	64, 175, 632, 921, 1176, 1267, 1366, 1481
LguI	GCTCTTC	1 cut(s)	1458
LmnI	GCTCC	3 cut(s)	306, 402, 1030
LweI	GCATC	8 cut(s)	331, 590, 673, 717, 902, 1081, 1414, 1594
MaeI	CTAG	5 cut(s)	293, 333, 764, 1203, 1272
MaeII	ACGT	3 cut(s)	358, 815, 861
MalI	GATC	8 cut(s)	66, 177, 634, 923, 1178, 1269, 1368, 1483
MboI	GATC	8 cut(s)	64, 175, 632, 921, 1176, 1267, 1366, 1481
MflI	RGATCY	2 cut(s)	175, 921
MhlI	GDGCHC	1 cut(s)	1263
MlsI	TGGCCA	1 cut(s)	797
MluCI	AATT	6 cut(s)	12, 328, 677, 965, 1228, 1307
MluNI	TGGCCA	1 cut(s)	797
MlyI	GAGTC	2 cut(s)	1012, 1463
MmeI	TCCRAC	3 cut(s)	424, 1020, 1437
Mox20I	TGGCCA	1 cut(s)	797
Mph1103I	ATGCAT	1 cut(s)	1012
MroNI	GCCGGC	1 cut(s)	1141
MroXI	GAANNNNTTC	1 cut(s)	1308
MscI	TGGCCA	1 cut(s)	797
MseI	TTAA	2 cut(s)	87, 390
Msp20I	TGGCCA	1 cut(s)	797
MspI	CCGG	1 cut(s)	1142
MspR9I	CCNGG	3 cut(s)	405, 536, 789
Mva1269I	GAATGC	1 cut(s)	1012
MvaI	CCWGG	3 cut(s)	405, 536, 789
MvnI	CGCG	1 cut(s)	613
MwoI	GCNNNNNNNGC	6 cut(s)	101, 236, 803, 1253, 1258, 1377
NaeI	GCCGGC	1 cut(s)	1143
NdeII	GATC	8 cut(s)	64, 175, 632, 921, 1176, 1267, 1366, 1481
NgoMIV	GCCGGC	1 cut(s)	1141
NlaIII	CATG	4 cut(s)	224, 431, 479, 1003
NlaIV	GGNNCC	3 cut(s)	201, 1239, 1261
NsiI	ATGCAT	1 cut(s)	1012
NspV	TTCGAA	1 cut(s)	1081
PagI	TCATGA	1 cut(s)	475
PauI	GCGCGC	1 cut(s)	611
PceI	AGGCCT	1 cut(s)	522
PciSI	GCTCTTC	1 cut(s)	1458
PctI	GAATGC	1 cut(s)	1012
PdiI	GCCGGC	1 cut(s)	1143
PdmI	GAANNNNTTC	1 cut(s)	1308
PfeI	GAWTC	2 cut(s)	1448, 1616
PkrI	GCNGC	1 cut(s)	688
Ple19I	CGATCG	1 cut(s)	1484
PleI	GAGTC	2 cut(s)	1012, 1462
PpsI	GAGTC	2 cut(s)	1012, 1462
Ppu21I	YACGTR	2 cut(s)	359, 816
PpuMI	RGGWCCY	1 cut(s)	199
Psp5II	RGGWCCY	1 cut(s)	199
Psp6I	CCWGG	3 cut(s)	403, 534, 787
PspFI	CCCAGC	2 cut(s)	1256, 1553
PspGI	CCWGG	3 cut(s)	403, 534, 787
PspN4I	GGNNCC	3 cut(s)	201, 1239, 1261
PspOMI	GGGCCC	1 cut(s)	1259
PspPI	GGNCC	7 cut(s)	49, 199, 490, 804, 1237, 1259, 1260
PspPPI	RGGWCCY	1 cut(s)	199
PsuI	RGATCY	2 cut(s)	175, 921
PteI	GCGCGC	1 cut(s)	611
PvuI	CGATCG	1 cut(s)	1484
RsaI	GTAC	3 cut(s)	209, 773, 818
RsaNI	GTAC	3 cut(s)	208, 772, 817
SalI	GTCGAC	1 cut(s)	542
SapI	GCTCTTC	1 cut(s)	1458
SaqAI	TTAA	2 cut(s)	87, 390
SatI	GCNGC	1 cut(s)	687
Sau3AI	GATC	8 cut(s)	64, 175, 632, 921, 1176, 1267, 1366, 1481
Sau96I	GGNCC	7 cut(s)	49, 199, 490, 804, 1237, 1259, 1260
SchI	GAGTC	2 cut(s)	1012, 1463
ScrFI	CCNGG	3 cut(s)	405, 536, 789
SduI	GDGCHC	1 cut(s)	1263
SfaNI	GCATC	8 cut(s)	331, 590, 673, 717, 902, 1081, 1414, 1594
SfcI	CTRYAG	1 cut(s)	31
SfiI	GGCCNNNNNGGCC	1 cut(s)	803
SfuI	TTCGAA	1 cut(s)	1081
SinI	GGWCC	4 cut(s)	49, 199, 490, 1237
SmlI	CTYRAG	1 cut(s)	671
SmoI	CTYRAG	1 cut(s)	671
Sse9I	AATT	6 cut(s)	12, 328, 677, 965, 1228, 1307
SseBI	AGGCCT	1 cut(s)	522
SsiI	CCGC	1 cut(s)	687
SspMI	CTAG	5 cut(s)	293, 333, 764, 1203, 1272
StuI	AGGCCT	1 cut(s)	522
StyD4I	CCNGG	3 cut(s)	403, 534, 787
TaaI	ACNGT	2 cut(s)	490, 1611
TaiI	ACGT	3 cut(s)	361, 818, 864
TaqI	TCGA	8 cut(s)	54, 353, 500, 515, 543, 1081, 1185, 1480
TasI	AATT	6 cut(s)	12, 328, 677, 965, 1228, 1307
TauI	GCSGC	1 cut(s)	689
TfiI	GAWTC	2 cut(s)	1448, 1616
Tru1I	TTAA	2 cut(s)	87, 390
Tru9I	TTAA	2 cut(s)	87, 390
TscAI	CASTG	3 cut(s)	102, 748, 1270
TspGWI	ACGGA	1 cut(s)	914
TspRI	CASTG	3 cut(s)	102, 748, 1270
VpaK11BI	GGWCC	4 cut(s)	49, 199, 490, 1237
XagI	CCTNNNNNAGG	1 cut(s)	56
XapI	RAATTY	3 cut(s)	12, 328, 1307
XcmI	CCANNNNNNNNNTGG	1 cut(s)	958
XmiI	GTMKAC	2 cut(s)	543, 1344
XmnI	GAANNNNTTC	1 cut(s)	1308
XspI	CTAG	5 cut(s)	293, 333, 764, 1203, 1272
Zsp2I	ATGCAT	1 cut(s)	1012

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.