Rh1BG118900

RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002471
Physical Location & Seq
Forward (+)
20316011 .. 20320204
4194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG118900.1

Sequence Viewer

Length: 855 bp
ATGCTTGAGAAATTAAATGCTACAAGAATACCCATTCAGCTCATCAAGGCTAACAACAAAAGGCTTCAACCAATGTCTCCATTGTTGTCATCTTTGCTAGTCAAGTATCCTGATACACTCCTGGGTCTGGCAAAAAGGGCCTTTATCACTTACTTGAAGTCCATTCAAAATCACAAGGACAAAGAAATTTTTAATCTGTCGGAACTGCCACTTAATGAATTTTCAGCATCTATGGGTCTACCAATGACCCCCAGAATCCGTTTTCCAAATCAGAAAATGAAGTCCACGACAGCGGGAGAACTATATCCTATTGGTGGACCAGAAAGTTCTGACATGGAGAACGGATTGGAGATTCCGAAAGAGGAACTTGATATTGGTGATTTGAATGAGGGGGAAGTGGATAACGATGTTCTTCTAACAAATGAGGGAACAGAGGAAGGAACAAAAACTGGAGATAATGTGCCAGCGACTCAAGTCATGAAGAGAAGGAATGGACCTATGGGAACTAGGACAGTCTTTGATGATTCGTGCAAGCCGATATCGCCCTTTGCAAAGATGGCTGGTACCGTACATGCTAGTGATCCCATATTGCTTGATGAACTTAAATACAAGAAACAAAAATATTATGATAAGGTTAGGGAAGAGTTGAAGAGATCAGAACCAGAAGACAAGATTCTAGACCGCCAGCGTTGCAATGAAAAACGAAGGAAAGAGAAGATGAAGAGGAGTAGAGTGAATGAAAGAGAAGAGGAACAGGATGATCTTTCTGATTCCAAAGAAGTGCCATATGAGTATCGACCCTGCAAAAGGGTAAAGAACGAAAAATGCAAGGAAAAAGAAGGGTTTCGCTGCTGA

Protein Analysis

284

Amino Acids

32.64

Weight (kDa)

8.41

Isoelectric Point (pI)

55.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTE_SPB4 PF13959 40 - 86 1.3e-12 ATP-dependent rRNA helicase SPB4-like, C-terminal extension
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 563
AccB1I GGYRCC 1 cut(s) 563
AccI GTMKAC 1 cut(s) 238
AciI CCGC 2 cut(s) 293, 682
AclWI GGATC 1 cut(s) 575
AcsI RAATTY 2 cut(s) 186, 218
AfaI GTAC 2 cut(s) 565, 570
AfiI CCNNNNNNNGG 3 cut(s) 127, 314, 807
AgsI TTSAA 5 cut(s) 68, 157, 167, 385, 649
AjnI CCWGG 1 cut(s) 120
AjuI GAANNNNNNNTTGG 2 cut(s) 357, 389
AluBI AGCT 1 cut(s) 40
AluI AGCT 1 cut(s) 40
Alw26I GTCTC 1 cut(s) 81
AlwI GGATC 1 cut(s) 575
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 1 cut(s) 849
ApoI RAATTY 2 cut(s) 186, 218
Asp700I GAANNNNTTC 1 cut(s) 843
Asp718I GGTACC 1 cut(s) 563
AspS9I GGNCC 3 cut(s) 138, 317, 494
AsuHPI GGTGA 1 cut(s) 389
AvaII GGWCC 2 cut(s) 317, 494
BanI GGYRCC 1 cut(s) 563
BbsI GAAGAC 1 cut(s) 672
BbvI GCAGC 1 cut(s) 836
BccI CCATC 1 cut(s) 550
BciT130I CCWGG 1 cut(s) 122
BciVI GTATCC 1 cut(s) 117
BcoDI GTCTC 1 cut(s) 81
BfaI CTAG 4 cut(s) 98, 507, 576, 677
BfuI GTATCC 1 cut(s) 117
BisI GCNGC 1 cut(s) 850
BlsI GCNGC 1 cut(s) 851
Bme1390I CCNGG 1 cut(s) 122
Bme18I GGWCC 2 cut(s) 317, 494
BmgT120I GGNCC 3 cut(s) 138, 317, 494
BmiI GGNNCC 1 cut(s) 565
BmrFI CCNGG 1 cut(s) 122
BmsI GCATC 1 cut(s) 236
BoxI GACNNNNGTC 1 cut(s) 473
BpiI GAAGAC 1 cut(s) 672
BpmI CTGGAG 1 cut(s) 471
BpuEI CTTGAG 2 cut(s) 26, 456
BsaJI CCNNGG 1 cut(s) 121
BsaXI ACNNNNNCTCC 2 cut(s) 444, 474
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 314, 807
Bse1I ACTGG 1 cut(s) 454
Bse3DI GCAATG 1 cut(s) 700
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 121
BseGI GGATG 1 cut(s) 763
BseLI CCNNNNNNNGG 3 cut(s) 127, 314, 807
BseMI GCAATG 1 cut(s) 700
BseNI ACTGG 1 cut(s) 454
BseRI GAGGAG 1 cut(s) 739
BseXI GCAGC 1 cut(s) 836
BshFI GGCC 1 cut(s) 140
BshNI GGYRCC 1 cut(s) 563
BslI CCNNNNNNNGG 3 cut(s) 127, 314, 807
BsmAI GTCTC 1 cut(s) 81
BsnI GGCC 1 cut(s) 140
Bsp143I GATC 3 cut(s) 580, 653, 760
BspACI CCGC 2 cut(s) 293, 682
BspANI GGCC 1 cut(s) 140
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 1 cut(s) 565
BspPI GGATC 1 cut(s) 575
BspT107I GGYRCC 1 cut(s) 563
BsrDI GCAATG 1 cut(s) 700
BsrI ACTGG 1 cut(s) 454
BssECI CCNNGG 1 cut(s) 121
BssMI GATC 3 cut(s) 580, 653, 760
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 2 cut(s) 514, 568
Bst6I CTCTTC 5 cut(s) 476, 636, 644, 716, 741
BstC8I GCNNGC 3 cut(s) 465, 533, 686
BstENI CCTNNNNNAGG 1 cut(s) 805
BstF5I GGATG 1 cut(s) 763
BstKTI GATC 3 cut(s) 583, 656, 763
BstMAI GTCTC 1 cut(s) 81
BstMBI GATC 3 cut(s) 580, 653, 760
BstMWI GCNNNNNNNGC 4 cut(s) 137, 541, 557, 690
BstNI CCWGG 1 cut(s) 122
BstNSI RCATGY 1 cut(s) 575
BstPAI GACNNNNGTC 1 cut(s) 473
BstSCI CCNGG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 836
BstV2I GAAGAC 1 cut(s) 672
BsuI GTATCC 1 cut(s) 117
BsuRI GGCC 1 cut(s) 140
BtsCI GGATG 1 cut(s) 763
Cac8I GCNNGC 3 cut(s) 465, 533, 686
CciI TCATGA 1 cut(s) 477
Cfr13I GGNCC 3 cut(s) 138, 317, 494
Csp6I GTAC 2 cut(s) 564, 569
CviAII CATG 3 cut(s) 334, 478, 572
CviJI RGCY 6 cut(s) 40, 50, 64, 140, 535, 560
CviKI_1 RGCY 6 cut(s) 40, 50, 64, 140, 535, 560
CviQI GTAC 2 cut(s) 564, 569
DpnI GATC 3 cut(s) 582, 655, 762
DpnII GATC 3 cut(s) 580, 653, 760
Eam1104I CTCTTC 5 cut(s) 476, 636, 644, 716, 741
EarI CTCTTC 5 cut(s) 476, 636, 644, 716, 741
Eco32I GATATC 1 cut(s) 540
Eco47I GGWCC 2 cut(s) 317, 494
EcoNI CCTNNNNNAGG 1 cut(s) 805
EcoO109I RGGNCCY 1 cut(s) 138
EcoRII CCWGG 1 cut(s) 120
EcoRV GATATC 1 cut(s) 540
FaeI CATG 3 cut(s) 337, 481, 575
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FatI CATG 3 cut(s) 333, 477, 571
FauI CCCGC 1 cut(s) 286
FauNDI CATATG 1 cut(s) 787
FblI GTMKAC 1 cut(s) 238
Fnu4HI GCNGC 1 cut(s) 850
FokI GGATG 1 cut(s) 770
Fsp4HI GCNGC 1 cut(s) 850
FspBI CTAG 4 cut(s) 98, 507, 576, 677
GluI GCNGC 1 cut(s) 850
GsuI CTGGAG 1 cut(s) 471
HaeIII GGCC 1 cut(s) 140
Hin1II CATG 3 cut(s) 337, 481, 575
HinfI GANTC 6 cut(s) 255, 352, 469, 524, 673, 770
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 3 cut(s) 239, 285, 317
Hpy188I TCNGA 6 cut(s) 202, 273, 331, 357, 658, 769
Hpy188III TCNNGA 3 cut(s) 110, 478, 677
Hpy8I GTNNAC 3 cut(s) 239, 285, 317
HpyAV CCTTC 4 cut(s) 431, 480, 699, 833
HpyCH4III ACNGT 2 cut(s) 514, 568
HpyCH4V TGCA 5 cut(s) 531, 551, 693, 804, 828
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 541, 557, 690
Hsp92II CATG 3 cut(s) 337, 481, 575
KpnI GGTACC 1 cut(s) 567
Kzo9I GATC 3 cut(s) 580, 653, 760
Lsp1109I GCAGC 1 cut(s) 836
LweI GCATC 1 cut(s) 236
MaeI CTAG 4 cut(s) 98, 507, 576, 677
MalI GATC 3 cut(s) 582, 655, 762
MboI GATC 3 cut(s) 580, 653, 760
MboII GAAGA 8 cut(s) 404, 493, 653, 661, 677, 727, 733, 758
MluCI AATT 3 cut(s) 11, 186, 218
MlyI GAGTC 1 cut(s) 463
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 6 cut(s) 355, 382, 418, 427, 717, 742
MroXI GAANNNNTTC 1 cut(s) 843
MseI TTAA 4 cut(s) 14, 192, 213, 603
MslI CAYNNNNRTG 1 cut(s) 576
MspA1I CMGCKG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 122
MvaI CCWGG 1 cut(s) 122
MwoI GCNNNNNNNGC 4 cut(s) 137, 541, 557, 690
NdeI CATATG 1 cut(s) 787
NdeII GATC 3 cut(s) 580, 653, 760
NlaIII CATG 3 cut(s) 337, 481, 575
NlaIV GGNNCC 1 cut(s) 565
NspI RCATGY 1 cut(s) 575
PagI TCATGA 1 cut(s) 477
PcsI WCGNNNNNNNCGW 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 843
PfeI GAWTC 5 cut(s) 255, 352, 524, 673, 770
PkrI GCNGC 1 cut(s) 851
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
PshAI GACNNNNGTC 1 cut(s) 473
Psp6I CCWGG 1 cut(s) 120
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 1 cut(s) 565
PspPI GGNCC 3 cut(s) 138, 317, 494
RsaI GTAC 2 cut(s) 565, 570
RsaNI GTAC 2 cut(s) 564, 569
RseI CAYNNNNRTG 1 cut(s) 576
SaqAI TTAA 4 cut(s) 14, 192, 213, 603
SatI GCNGC 1 cut(s) 850
Sau3AI GATC 3 cut(s) 580, 653, 760
Sau96I GGNCC 3 cut(s) 138, 317, 494
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 1 cut(s) 122
SetI ASST 3 cut(s) 42, 499, 636
SfaNI GCATC 1 cut(s) 236
SinI GGWCC 2 cut(s) 317, 494
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 2 cut(s) 5, 471
SmoI CTYRAG 2 cut(s) 5, 471
Sse9I AATT 3 cut(s) 11, 186, 218
SsiI CCGC 2 cut(s) 293, 682
SspI AATATT 1 cut(s) 623
SspMI CTAG 4 cut(s) 98, 507, 576, 677
StyD4I CCNGG 1 cut(s) 120
TaaI ACNGT 2 cut(s) 514, 568
TaqI TCGA 1 cut(s) 796
TasI AATT 3 cut(s) 11, 186, 218
TfiI GAWTC 5 cut(s) 255, 352, 524, 673, 770
Tru1I TTAA 4 cut(s) 14, 192, 213, 603
Tru9I TTAA 4 cut(s) 14, 192, 213, 603
TseI GCWGC 1 cut(s) 849
TspDTI ATGAA 7 cut(s) 231, 293, 494, 612, 711, 734, 753
TspGWI ACGGA 2 cut(s) 248, 357
VpaK11BI GGWCC 2 cut(s) 317, 494
XagI CCTNNNNNAGG 1 cut(s) 805
XapI RAATTY 2 cut(s) 186, 218
XbaI TCTAGA 1 cut(s) 676
XceI RCATGY 1 cut(s) 575
XmiI GTMKAC 1 cut(s) 238
XmnI GAANNNNTTC 1 cut(s) 843
XspI CTAG 4 cut(s) 98, 507, 576, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.