Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003616

Physical Location & Seq

Genomic Coordinates Reverse (-)

4960177 .. 4964680

Gene Structure 4504 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1063300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2157 bp

ATGGCTTTGAGGCGCCTGATCACTCAGGTTTCCAGGCATCGATCAGAGTTGGGACAATTGACAAAAGTGGTTCCCAGAACTTATTTTCCTGCCAACAGGTTAGGAGGAGGTGCCGGAGGGAATAAGTTTTTAAGTACTCAAGAAAGGTTCAAGTCCAGTTATGTTGGCAATTTTTCTCGTAGAGTACGCGATACCGATGAAGCTAGTGAAATTTCCTATCTAAAAGAACTTTACCATCGGGGTGATCATGAAGCAGTGATAAGAGCATTTGAAAGTCAGCCATCATTACATTCTAACCCTTCAGCACTTTCTGAATATGTGAAAGCATTAGTAAGAGTTGGCAGGCTGGATGAGAGTGAGTTACTCAAGACATTGCAGAGAGGCGTCGCCAACTCTGCAAGGGAGGAAGAAAGCTTTGCTGGCTTCTCAGTTCTCAGTAATGTTGGAAAATCAACAAAAGAAGGTATTCTTGGAACTGCAAGTGCTCCTATTCATATGGTGGCTAGAGAAGGAGGTCAGTTTAAGGAGCAGCTGTGGCGTACGATTCGCACTATCGCACTGGCCTTTCTTTTGATATCTGGTGTAGGAGCACTCATTGAGGATAGAGGAATAAGCAAAGGACTTGGACTAAATGAGGAGGTTCAACCTAGCCTGGAATCAAACACAAAATTTAGTGATGTAAAGGGTGTTGATGAAGCTAAATCCGAGTTAGAGGAAATTGTTCACTATCTGCGAGATCCCAAGCGTTTTACTCGTCTAGGTGGCAAGCTCCCAAAAGGTGTTTTGCTTGTTGGTCCACCTGGCACTGGAAAAACCATGTTAGCAAGAGCCATTGCTGGAGAAGCAGGGGTGCCTTTCTTTTCATGCAGCGGCAGCGAGTTTGAAGAGATGTTTGTTGGTGTTGGGGCTCGAAGGGTGAGGGATCTTTTCTCGGCTGCAAAGAAACGGTCACCTTGTATAATATTCATTGATGAGATAGACGCAATAGGAGGAAGCCGGAACCCAAAGGATCAGCAGTACATGAAGATGACCCTTAATCAGTTACTTGTTGAACTTGATGGTTTTAAACAAAATGAAGGGATTATTGTCATTGCTGCTACTAATTTCCCAGAGTCTTTGGACAAGGCATTGGTGAGACCTGGACGGTTTGACCGGCACATTGTTGTCCCTAATCCTGATGTTGAAGGGCGAAGGCAGATTATGGAAGCCCACATGTCGAAGGTTCTGAAGGCCAATGACGTTGATTTGAGTATTATTGCTAGAGGAACTCCGGGGTTCTCCGGAGCTGATCTTGCGAACTTGATCAACATTGCTGCTCTGAAGGCTGCCATGGACGGTGCTAAAGCAGTGAACATGGCTGACCTTGAATATGCAAAAGACAAGATTATGATGGGAAGCGAACGCAAGTCGGCTGTTATATCTGATGAGTCAAGAAAGCTTACAGCTTTTCACGAGGGTGGCCATGCACTTGTAGCTATCCACACCGATGGTGCTCATCCAGTTCACAAGGCAACAATAGTTCCTAGGGGTATGTCGCTTGGCATGGTTGCACAGCTACCCGAAAAGGACGAGACAAGTGTCTCCCGGAAACAGATGCTTGCACGGCTCGACGTTTGTATGGGGGGGCGTGTTGCAGAAGAGCTCATCTTTGGAGAAAGTGAAGTTACTTCCGGTGCATCAGATGATCTCCGGCAAGCTACTTCGCTTGCTAGAGCAATGGTTACAAAGTATGGTATGAGCAAACAAGTTGGACTCGTCAGTCACAATTACGACGACAATGGTAAGAGTATGAGCACAGAAACTAGGCTGCTCATTGAAGAAGAAGTAAAGAACCTCTTGGAAAAGGCATACAACAATGCAAAAACCATCCTCACGGAACACAGCAAGGAACTCTACGCCCTCGCTAATGCTTTACTAGAGGAAGAAACACTGACCGGAACTCAGATCAAGTCCTTGCTCGCACAAGTAAACTCCCAGCAACAGCAGCAGCAGCAACCGCACCAGCAAGTAGTTGCAGCAAAGAATAACTCGCAGTCTAAGCCAGTGCCACCCTCCACACCCAACGCGGCTGCATCTGCTGCTGCTGCTGCAGCCGCAGCTGCAGCTGCCGCAACTGCTGCGGCCAAGTCAACAGGCATTGCTCCTGCCGGATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009536 GO:0009888 GO:0009941 GO:0010073 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048507 GO:0048856 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04139 map04139

Pfam Domains

Protein Families

AAA Peptidase_M41

Protein Analysis

719

Amino Acids

77.45

Weight (kDa)

8.74

Isoelectric Point (pI)

43.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIP49	PF06068	206 - 294	6.4e-06	TIP49 P-loop domain
AAA_5	PF07728	260 - 330	5.7e-06	AAA domain (dynein-related subfamily)
AAA	PF00004	261 - 390	2.7e-46	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	413 - 456	2.8e-12	AAA+ lid domain
Peptidase_M41	PF01434	472 - 652	4.3e-67	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003616)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26140
fragaria_vesca	FvH4_1g07720 FvH4_2g31730 FvH4_2g31730
malus_domestica	MD08G1063300.v1.1 MD15G1058100.v1.1 MD15G1058500.v1.1
prunus_persica	Prupe.1G410800_v2.0.a1
pyrus_communis	pycom08g05200 pycom15g05470 pycom15g05500
rosa_chinensis	RchiOBHm_Chr2g0093601 RchiOBHm_Chr6g0311091
rosa_laevigata	RLG00000010436 RLG00000016420
rosa_multiflora	Rmu_co8327861.1_g000001 Rmu_co8491229.1_g000001 Rmu_sc0002126.1_g000022 Rmu_sc0008967.1_g000002 Rmu_sc0012235.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147960 Rroxscaffold_7G00158030
rosa_rugosa	Rorug02G0037800 Rorug02G0037800 Rorug06G0385300 Rorug06G0385400
rosa_samantha	Rh2AG084300 Rh2BG084900 Rh2CG087200 Rh2DG082900 Rh6AG499400 Rh6BG509900 Rh6CG515700 Rh6DG502300
rosa_wichuraiana	Rw6G043500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1758
AccB1I	GGYRCC	3 cut(s)	12, 110, 850
AccII	CGCG	2 cut(s)	189, 2066
AccIII	TCCGGA	1 cut(s)	1280
AciI	CCGC	6 cut(s)	870, 1997, 2066, 2094, 2109, 2120
AclWI	GGATC	3 cut(s)	731, 930, 1017
AcoI	YGGCCR	2 cut(s)	1459, 2121
AcsI	RAATTY	2 cut(s)	210, 668
AcuI	CTGAAG	3 cut(s)	285, 1247, 1340
AcyI	GRCGYC	2 cut(s)	13, 384
AfaI	GTAC	4 cut(s)	136, 186, 541, 1019
AfiI	CCNNNNNNNGG	1 cut(s)	806
AflIII	ACRYGT	1 cut(s)	1212
AgsI	TTSAA	8 cut(s)	151, 272, 644, 884, 1052, 1184, 1367, 1817
AjnI	CCWGG	4 cut(s)	32, 651, 799, 1138
AjuI	GAANNNNNNNTTGG	2 cut(s)	453, 485
AleI	CACNNNNGTG	1 cut(s)	1455
AloI	GAACNNNNNNTCC	2 cut(s)	70, 102
Alw21I	GWGCWC	5 cut(s)	487, 592, 1495, 1644, 1796
Alw26I	GTCTC	3 cut(s)	1129, 1565, 1585
AlwI	GGATC	3 cut(s)	731, 930, 1017
Aor13HI	TCCGGA	1 cut(s)	1280
AoxI	GGCC	4 cut(s)	561, 1230, 1459, 2121
ApoI	RAATTY	2 cut(s)	210, 668
ArsI	GACNNNNNNTTYG	2 cut(s)	932, 964
Asp700I	GAANNNNTTC	2 cut(s)	465, 720
AspA2I	CCTAGG	1 cut(s)	1523
AspLEI	GCGC	1 cut(s)	15
AspS9I	GGNCC	1 cut(s)	794
AsuC2I	CCSGG	2 cut(s)	1272, 1585
AsuHPI	GGTGA	4 cut(s)	254, 928, 942, 1144
AvaII	GGWCC	1 cut(s)	794
AvrII	CCTAGG	1 cut(s)	1523
BalI	TGGCCA	1 cut(s)	1461
BanI	GGYRCC	3 cut(s)	12, 110, 850
BanII	GRGCYC	2 cut(s)	910, 1644
BauI	CACGAG	1 cut(s)	1451
Bbv12I	GWGCWC	5 cut(s)	487, 592, 1495, 1644, 1796
BccI	CCATC	6 cut(s)	243, 289, 1052, 1384, 1481, 1874
BceAI	ACGGC	1 cut(s)	1619
BciT130I	CCWGG	4 cut(s)	34, 653, 801, 1140
BclI	TGATCA	3 cut(s)	18, 244, 1302
BcnI	CCSGG	2 cut(s)	1272, 1585
BcoDI	GTCTC	3 cut(s)	1129, 1565, 1585
BfaI	CTAG	9 cut(s)	204, 504, 648, 758, 1260, 1524, 1710, 1803, 1916
BfmI	CTRYAG	2 cut(s)	2088, 2100
BfoI	RGCGCY	1 cut(s)	16
BlnI	CCTAGG	1 cut(s)	1523
BmcAI	AGTACT	1 cut(s)	136
Bme1390I	CCNGG	6 cut(s)	34, 653, 801, 1140, 1272, 1585
Bme18I	GGWCC	1 cut(s)	794
BmgT120I	GGNCC	1 cut(s)	794
BmiI	GGNNCC	5 cut(s)	14, 72, 112, 852, 1001
BmrFI	CCNGG	6 cut(s)	34, 653, 801, 1140, 1272, 1585
BmsI	GCATC	4 cut(s)	46, 1584, 1685, 2081
BoxI	GACNNNNGTC	1 cut(s)	1577
BpmI	CTGGAG	1 cut(s)	858
BpuEI	CTTGAG	2 cut(s)	123, 350
BpuMI	CCSGG	2 cut(s)	1272, 1585
Bsa29I	ATCGAT	1 cut(s)	40
BsaHI	GRCGYC	2 cut(s)	13, 384
BsaI	GGTCTC	1 cut(s)	1129
BsaJI	CCNNGG	3 cut(s)	1271, 1329, 1523
BsaWI	WCCGGW	3 cut(s)	1280, 1670, 1934
BsaXI	ACNNNNNCTCC	2 cut(s)	518, 548
Bsc4I	CCNNNNNNNGG	1 cut(s)	806
Bse118I	RCCGGY	1 cut(s)	1152
Bse1I	ACTGG	5 cut(s)	156, 564, 811, 1499, 2042
Bse3DI	GCAATG	6 cut(s)	371, 831, 1089, 1308, 1722, 2136
BseAI	TCCGGA	1 cut(s)	1280
BseBI	CCWGG	4 cut(s)	34, 653, 801, 1140
BseCI	ATCGAT	1 cut(s)	40
BseDI	CCNNGG	3 cut(s)	1271, 1329, 1523
BseGI	GGATG	3 cut(s)	355, 1495, 1866
BseLI	CCNNNNNNNGG	1 cut(s)	806
BseMI	GCAATG	6 cut(s)	371, 831, 1089, 1308, 1722, 2136
BseMII	CTCAG	4 cut(s)	38, 441, 448, 1955
BseNI	ACTGG	5 cut(s)	156, 564, 811, 1499, 2042
BseRI	GAGGAG	2 cut(s)	120, 650
BseYI	CCCAGC	1 cut(s)	1974
Bsh1236I	CGCG	2 cut(s)	189, 2066
BshFI	GGCC	4 cut(s)	563, 1232, 1461, 2123
BshNI	GGYRCC	3 cut(s)	12, 110, 850
BshVI	ATCGAT	1 cut(s)	40
BsiHKAI	GWGCWC	5 cut(s)	487, 592, 1495, 1644, 1796
BsiWI	CGTACG	1 cut(s)	539
BslFI	GGGAC	2 cut(s)	66, 1151
BslI	CCNNNNNNNGG	1 cut(s)	806
BsmAI	GTCTC	3 cut(s)	1129, 1565, 1585
BsmFI	GGGAC	2 cut(s)	66, 1151
BsnI	GGCC	4 cut(s)	563, 1232, 1461, 2123
Bso31I	GGTCTC	1 cut(s)	1129
Bsp1286I	GDGCHC	6 cut(s)	487, 592, 910, 1495, 1644, 1796
Bsp13I	TCCGGA	1 cut(s)	1280
Bsp19I	CCATGG	1 cut(s)	1329
BspACI	CCGC	6 cut(s)	870, 1997, 2066, 2094, 2109, 2120
BspANI	GGCC	4 cut(s)	563, 1232, 1461, 2123
BspCNI	CTCAG	4 cut(s)	37, 440, 447, 1954
BspDI	ATCGAT	1 cut(s)	40
BspEI	TCCGGA	1 cut(s)	1280
BspFNI	CGCG	2 cut(s)	189, 2066
BspHI	TCATGA	1 cut(s)	247
BspLI	GGNNCC	5 cut(s)	14, 72, 112, 852, 1001
BspMAI	CTGCAG	2 cut(s)	2092, 2104
BspPI	GGATC	3 cut(s)	731, 930, 1017
BspQI	GCTCTTC	1 cut(s)	1632
BspT107I	GGYRCC	3 cut(s)	12, 110, 850
BspTNI	GGTCTC	1 cut(s)	1129
BsrDI	GCAATG	6 cut(s)	371, 831, 1089, 1308, 1722, 2136
BsrFI	RCCGGY	1 cut(s)	1152
BsrI	ACTGG	5 cut(s)	156, 564, 811, 1499, 2042
BssAI	RCCGGY	1 cut(s)	1152
BssECI	CCNNGG	3 cut(s)	1271, 1329, 1523
BssNI	GRCGYC	2 cut(s)	13, 384
BssSI	CACGAG	1 cut(s)	1451
BssT1I	CCWWGG	2 cut(s)	1329, 1523
Bst2BI	CACGAG	1 cut(s)	1451
Bst2UI	CCWGG	4 cut(s)	34, 653, 801, 1140
Bst4CI	ACNGT	3 cut(s)	948, 1146, 1337
Bst6I	CTCTTC	2 cut(s)	879, 1632
BstACI	GRCGYC	2 cut(s)	13, 384
BstAPI	GCANNNNNTGC	2 cut(s)	2078, 2117
BstC8I	GCNNGC	7 cut(s)	344, 421, 767, 1599, 1695, 1707, 1959
BstDEI	CTNAG	6 cut(s)	24, 427, 434, 1941, 2037, 2154
BstDSI	CCRYGG	1 cut(s)	1329
BstEII	GGTNACC	1 cut(s)	948
BstF5I	GGATG	3 cut(s)	355, 1495, 1866
BstFNI	CGCG	2 cut(s)	189, 2066
BstH2I	RGCGCY	1 cut(s)	16
BstHHI	GCGC	1 cut(s)	15
BstMAI	GTCTC	3 cut(s)	1129, 1565, 1585
BstNI	CCWGG	4 cut(s)	34, 653, 801, 1140
BstNSI	RCATGY	1 cut(s)	1216
BstPAI	GACNNNNGTC	1 cut(s)	1577
BstPI	GGTNACC	1 cut(s)	948
BstSCI	CCNGG	6 cut(s)	32, 651, 799, 1138, 1270, 1583
BstSFI	CTRYAG	2 cut(s)	2088, 2100
BstUI	CGCG	2 cut(s)	189, 2066
BstX2I	RGATCY	3 cut(s)	736, 922, 2150
BstXI	CCANNNNNNTGG	1 cut(s)	1487
BstYI	RGATCY	3 cut(s)	736, 922, 2150
Bsu15I	ATCGAT	1 cut(s)	40
BsuRI	GGCC	4 cut(s)	563, 1232, 1461, 2123
BsuTUI	ATCGAT	1 cut(s)	40
BtgI	CCRYGG	1 cut(s)	1329
BtsCI	GGATG	3 cut(s)	355, 1495, 1866
BtsI	GCAGTG	2 cut(s)	261, 1353
BtsIMutI	CAGTG	6 cut(s)	261, 557, 804, 1353, 1928, 2049
Cac8I	GCNNGC	7 cut(s)	344, 421, 767, 1599, 1695, 1707, 1959
CciI	TCATGA	1 cut(s)	247
CfoI	GCGC	1 cut(s)	15
Cfr10I	RCCGGY	1 cut(s)	1152
Cfr13I	GGNCC	1 cut(s)	794
ClaI	ATCGAT	1 cut(s)	40
CseI	GACGC	2 cut(s)	373, 989
Csp6I	GTAC	4 cut(s)	135, 185, 540, 1018
CviAII	CATG	9 cut(s)	248, 817, 864, 1021, 1213, 1330, 1354, 1463, 1543
CviQI	GTAC	4 cut(s)	135, 185, 540, 1018
DdeI	CTNAG	6 cut(s)	24, 427, 434, 1941, 2037, 2154
DinI	GGCGCC	1 cut(s)	14
DraI	TTTAAA	1 cut(s)	1066
DrdI	GACNNNNNNGTC	1 cut(s)	1758
DseDI	GACNNNNNNGTC	1 cut(s)	1758
EaeI	YGGCCR	2 cut(s)	1459, 2121
Eam1104I	CTCTTC	2 cut(s)	879, 1632
EarI	CTCTTC	2 cut(s)	879, 1632
Ecl136II	GAGCTC	1 cut(s)	1642
Eco130I	CCWWGG	2 cut(s)	1329, 1523
Eco24I	GRGCYC	2 cut(s)	910, 1644
Eco31I	GGTCTC	1 cut(s)	1129
Eco32I	GATATC	1 cut(s)	576
Eco47I	GGWCC	1 cut(s)	794
Eco53kI	GAGCTC	1 cut(s)	1642
Eco57I	CTGAAG	3 cut(s)	285, 1247, 1340
Eco91I	GGTNACC	1 cut(s)	948
EcoICRI	GAGCTC	1 cut(s)	1642
EcoO65I	GGTNACC	1 cut(s)	948
EcoRII	CCWGG	4 cut(s)	32, 651, 799, 1138
EcoRV	GATATC	1 cut(s)	576
EcoT14I	CCWWGG	2 cut(s)	1329, 1523
EcoT38I	GRGCYC	2 cut(s)	910, 1644
EgeI	GGCGCC	1 cut(s)	14
EheI	GGCGCC	1 cut(s)	14
ErhI	CCWWGG	2 cut(s)	1329, 1523
FaeI	CATG	9 cut(s)	251, 820, 867, 1024, 1216, 1333, 1357, 1466, 1546
FalI	AAGNNNNNCTT	4 cut(s)	453, 485, 1820, 1852
FaqI	GGGAC	2 cut(s)	66, 1151
FatI	CATG	9 cut(s)	247, 816, 863, 1020, 1212, 1329, 1353, 1462, 1542
FauNDI	CATATG	1 cut(s)	495
FbaI	TGATCA	3 cut(s)	18, 244, 1302
FokI	GGATG	3 cut(s)	362, 1482, 1853
FriOI	GRGCYC	2 cut(s)	910, 1644
FspBI	CTAG	9 cut(s)	204, 504, 648, 758, 1260, 1524, 1710, 1803, 1916
GlaI	GCGC	1 cut(s)	14
GsaI	CCCAGC	1 cut(s)	1978
GsuI	CTGGAG	1 cut(s)	858
HaeII	RGCGCY	1 cut(s)	16
HaeIII	GGCC	4 cut(s)	563, 1232, 1461, 2123
HgaI	GACGC	2 cut(s)	373, 989
HhaI	GCGC	1 cut(s)	15
Hin1I	GRCGYC	2 cut(s)	13, 384
Hin1II	CATG	9 cut(s)	251, 820, 867, 1024, 1216, 1333, 1357, 1466, 1546
Hin6I	GCGC	1 cut(s)	13
HinP1I	GCGC	1 cut(s)	13
HincII	GTYRAC	1 cut(s)	2130
HindII	GTYRAC	1 cut(s)	2130
HindIII	AAGCTT	2 cut(s)	412, 1436
HinfI	GANTC	5 cut(s)	544, 656, 1112, 1427, 1752
HphI	GGTGA	4 cut(s)	254, 928, 942, 1144
Hpy166II	GTNNAC	6 cut(s)	724, 797, 1351, 1504, 1969, 2130
Hpy188I	TCNGA	8 cut(s)	46, 313, 706, 1227, 1320, 1423, 1681, 1944
Hpy188III	TCNNGA	7 cut(s)	140, 248, 367, 1175, 1281, 1431, 1451
Hpy8I	GTNNAC	6 cut(s)	724, 797, 1351, 1504, 1969, 2130
Hpy99I	CGWCG	3 cut(s)	389, 1613, 1775
HpyCH4III	ACNGT	3 cut(s)	948, 1146, 1337
HpyCH4IV	ACGT	2 cut(s)	1239, 1611
HpyF3I	CTNAG	6 cut(s)	24, 427, 434, 1941, 2037, 2154
HpySE526I	ACGT	2 cut(s)	1239, 1611
Hsp92I	GRCGYC	2 cut(s)	13, 384
Hsp92II	CATG	9 cut(s)	251, 820, 867, 1024, 1216, 1333, 1357, 1466, 1546
HspAI	GCGC	1 cut(s)	13
KasI	GGCGCC	1 cut(s)	12
Kpn2I	TCCGGA	1 cut(s)	1280
Ksp22I	TGATCA	3 cut(s)	18, 244, 1302
LguI	GCTCTTC	1 cut(s)	1632
LmnI	GCTCC	6 cut(s)	490, 526, 587, 774, 1283, 2146
LweI	GCATC	4 cut(s)	46, 1584, 1685, 2081
MaeI	CTAG	9 cut(s)	204, 504, 648, 758, 1260, 1524, 1710, 1803, 1916
MaeII	ACGT	2 cut(s)	1239, 1611
MaeIII	GTNAC	6 cut(s)	360, 948, 1041, 1663, 1720, 1760
MboII	GAAGA	7 cut(s)	419, 896, 1036, 1649, 1829, 1832, 1934
MfeI	CAATTG	1 cut(s)	56
MflI	RGATCY	3 cut(s)	736, 922, 2150
MhlI	GDGCHC	6 cut(s)	487, 592, 910, 1495, 1644, 1796
MlsI	TGGCCA	1 cut(s)	1461
MluCI	AATT	7 cut(s)	56, 169, 210, 668, 717, 1102, 1765
MluNI	TGGCCA	1 cut(s)	1461
Mly113I	GGCGCC	1 cut(s)	13
MlyI	GAGTC	3 cut(s)	1121, 1436, 1746
MmeI	TCCRAC	2 cut(s)	424, 1729
Mox20I	TGGCCA	1 cut(s)	1461
MroI	TCCGGA	1 cut(s)	1280
MroXI	GAANNNNTTC	2 cut(s)	465, 720
MscI	TGGCCA	1 cut(s)	1461
MseI	TTAA	4 cut(s)	131, 522, 1035, 1065
MslI	CAYNNNNRTG	4 cut(s)	240, 1025, 1455, 1485
Msp20I	TGGCCA	1 cut(s)	1461
MspA1I	CMGCKG	4 cut(s)	532, 870, 2099, 2105
MspR9I	CCNGG	6 cut(s)	34, 653, 801, 1140, 1272, 1585
MunI	CAATTG	1 cut(s)	56
MvaI	CCWGG	4 cut(s)	34, 653, 801, 1140
MvnI	CGCG	2 cut(s)	189, 2066
NarI	GGCGCC	1 cut(s)	13
NciI	CCSGG	2 cut(s)	1272, 1585
NcoI	CCATGG	1 cut(s)	1329
NdeI	CATATG	1 cut(s)	495
NlaIII	CATG	9 cut(s)	251, 820, 867, 1024, 1216, 1333, 1357, 1466, 1546
NlaIV	GGNNCC	5 cut(s)	14, 72, 112, 852, 1001
NmeAIII	GCCGAG	1 cut(s)	911
NmuCI	GTSAC	2 cut(s)	948, 1760
NspI	RCATGY	1 cut(s)	1216
OliI	CACNNNNGTG	1 cut(s)	1455
PagI	TCATGA	1 cut(s)	247
PciI	ACATGT	1 cut(s)	1212
PciSI	GCTCTTC	1 cut(s)	1632
PdmI	GAANNNNTTC	2 cut(s)	465, 720
PfeI	GAWTC	2 cut(s)	544, 656
Pfl23II	CGTACG	1 cut(s)	539
PfoI	TCCNGGA	1 cut(s)	1583
PleI	GAGTC	3 cut(s)	1120, 1435, 1746
PluTI	GGCGCC	1 cut(s)	16
PpsI	GAGTC	3 cut(s)	1120, 1435, 1746
PscI	ACATGT	1 cut(s)	1212
PshAI	GACNNNNGTC	1 cut(s)	1577
Psp124BI	GAGCTC	1 cut(s)	1644
Psp6I	CCWGG	4 cut(s)	32, 651, 799, 1138
PspEI	GGTNACC	1 cut(s)	948
PspFI	CCCAGC	1 cut(s)	1974
PspGI	CCWGG	4 cut(s)	32, 651, 799, 1138
PspLI	CGTACG	1 cut(s)	539
PspN4I	GGNNCC	5 cut(s)	14, 72, 112, 852, 1001
PspPI	GGNCC	1 cut(s)	794
PstI	CTGCAG	2 cut(s)	2092, 2104
PsuI	RGATCY	3 cut(s)	736, 922, 2150
PvuII	CAGCTG	3 cut(s)	532, 2099, 2105
RsaI	GTAC	4 cut(s)	136, 186, 541, 1019
RsaNI	GTAC	4 cut(s)	135, 185, 540, 1018
RseI	CAYNNNNRTG	4 cut(s)	240, 1025, 1455, 1485
SacI	GAGCTC	1 cut(s)	1644
SapI	GCTCTTC	1 cut(s)	1632
SaqAI	TTAA	4 cut(s)	131, 522, 1035, 1065
Sau96I	GGNCC	1 cut(s)	794
ScaI	AGTACT	1 cut(s)	136
SchI	GAGTC	3 cut(s)	1121, 1436, 1746
ScrFI	CCNGG	6 cut(s)	34, 653, 801, 1140, 1272, 1585
SduI	GDGCHC	6 cut(s)	487, 592, 910, 1495, 1644, 1796
SfaNI	GCATC	4 cut(s)	46, 1584, 1685, 2081
SfcI	CTRYAG	2 cut(s)	2088, 2100
SfoI	GGCGCC	1 cut(s)	14
SinI	GGWCC	1 cut(s)	794
SmiMI	CAYNNNNRTG	4 cut(s)	240, 1025, 1455, 1485
SmlI	CTYRAG	2 cut(s)	138, 365
SmoI	CTYRAG	2 cut(s)	138, 365
Sse9I	AATT	7 cut(s)	56, 169, 210, 668, 717, 1102, 1765
SsiI	CCGC	6 cut(s)	870, 1997, 2066, 2094, 2109, 2120
SspDI	GGCGCC	1 cut(s)	12
SspI	AATATT	1 cut(s)	963
SspMI	CTAG	9 cut(s)	204, 504, 648, 758, 1260, 1524, 1710, 1803, 1916
SstI	GAGCTC	1 cut(s)	1644
StyD4I	CCNGG	6 cut(s)	32, 651, 799, 1138, 1270, 1583
StyI	CCWWGG	2 cut(s)	1329, 1523
TaaI	ACNGT	3 cut(s)	948, 1146, 1337
TaiI	ACGT	2 cut(s)	1242, 1614
TaqI	TCGA	4 cut(s)	40, 910, 1217, 1608
TasI	AATT	7 cut(s)	56, 169, 210, 668, 717, 1102, 1765
TatI	WGTACW	2 cut(s)	134, 1017
TauI	GCSGC	5 cut(s)	873, 2069, 2096, 2111, 2123
TfiI	GAWTC	2 cut(s)	544, 656
Tru1I	TTAA	4 cut(s)	131, 522, 1035, 1065
Tru9I	TTAA	4 cut(s)	131, 522, 1035, 1065
TscAI	CASTG	6 cut(s)	261, 564, 811, 1353, 1935, 2049
TseFI	GTSAC	2 cut(s)	948, 1760
Tsp45I	GTSAC	2 cut(s)	948, 1760
TspDTI	ATGAA	8 cut(s)	213, 264, 482, 708, 852, 955, 1037, 1089
TspGWI	ACGGA	1 cut(s)	1889
TspRI	CASTG	6 cut(s)	261, 564, 811, 1353, 1935, 2049
VpaK11BI	GGWCC	1 cut(s)	794
XapI	RAATTY	2 cut(s)	210, 668
XceI	RCATGY	1 cut(s)	1216
XmaJI	CCTAGG	1 cut(s)	1523
XmnI	GAANNNNTTC	2 cut(s)	465, 720
XspI	CTAG	9 cut(s)	204, 504, 648, 758, 1260, 1524, 1710, 1803, 1916
ZrmI	AGTACT	1 cut(s)	136

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.