Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003616

Physical Location & Seq

Genomic Coordinates Forward (+)

3904277 .. 3909404

Gene Structure 5128 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1058100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2151 bp

ATGGCTTTGAGGCGCCTGATCACTCAGGTTTCAAGGCATCGATCAGAGTTGGGACAACTGACAAAAGTGGTTGCCAGAACTTATCATCCAACCAACAGGTTCGGAGGAGGTGCCGGAGGGAACAAGTTTTTCAGTGCTCAAGAAAGGTTCAAGTCGAGCTATGTTGGCAATTTCGCTCGTAGAGTACGCGATACCGATGAAGCTAGTGAAATTTCCTATCTAAAAGAACTTTACAATCGGAGTGATTATGAAGCAGTGATAAGAGCATTTGAAAGTCAACCATCATTACATTCTAACCCTTCAGCACTTTCTGAATATGTGAAAGCATTAGTAAGAGTTGGCAGGCTGGACGAGAGTGAGTTACTCAAGACATTGCAGAGAGGTGTTGCTAACTCTGCAAGGGAGGAAGAAAGCATTGCCGGCTTCTCAGTTCTCAGGAATTTTGGAAAATCAACAAAAGAAGGTATTCTTGGAACTGCAAGTGCTCCTATTCATATGGTGGCTACAGAAGGAGGTCAGTTTAAAGAGCAGCTGTGGCGTACAATTCGCACAATCGCACTGGCCTTCCTTATGATATCTGGTATTGGAGCACTCATTGAGGATAAAGGAATAAGCAAAGGACTCGGACTAAATGAGGAGGTTCAACCTAGCATGGAATCAAACACAAAATTTAGCGATGTTAAGGGTGTTGATGAGGCTAAATCCGAGTTAGAGGAAATTGTTCACTATCTTCGGGATCCTAAGCGTTTTACTCGTCTAGGTGGAAAGCTCCCAAAAGGTGTTTTACTTGTTGGTCCACCTGGCACAGGAAAAACCATGTTAGCAAGAGCCATTGCTGGAGAAGCAGGGGTTCCTTTCTATTCATGCAGTGGCAGTGAGTTTGAAGAGATGTATGTTGGTGTTGGGGCTCGAAGGGTGAGGGATCTTTTCTCAGCTGCAAAGAAGCGGTCACCTTGTATAATATTCATTGATGAGATAGACGCAATAGGAGGAAGCCGAAATCCAAAGGATATGCAGTACATGAAAATGACCCTTAATCAGTTACTTGTTGAACTTGATGGTTTTAAACAAAATGAAGGGATTATTGTCATTGCTGCTACTAATTTCCCAGAGTCTCTGGACAAGGCATTGGTGAGACCTGGACGGTTTGATCGGCATATTGTTGTTCCTAATCCTGACGTTGAAGGGCGAAGGCAGATTATGGAAGCCCACATGTCGAAGGTTCTGAAGGCCAATGACGTCGATTTGAGTATTATTGCTAGAGGAACTCCAGGGTTCTCCGGAGCTGATCTTGCGAACTTGATCAACGTTGCTGCTCTGAAGGCTGCCATGGACGGTTCTAAAGCAGTGAATATGGCTGATCTTGAATATGCAAAAGACAAGATTATGATGGGAAGCGAACGCAAGTCAGCCGTTATATCTGATGAGTCAAGAAAGCTAACAGCTTTTCATGAGGGTGGGCATGCACTTGTAGCAATCCACACCGATGGTGCTCTTCCAGTTCACAAGGCAACAATAGTTCCTAGGGGTATGGCGCTTGGCATGGTTGCCCTGCTGCCCGAGAAGGACCAGACAAGTGTCTCGCGGAAACAGATGCTTGCAGAGCTCGACGTTTGTATGGGGGGGCGTGTTGCAGAAGAGCTCATCTTTGGAGAAAGTGAAGTCACTTCCGGTGCATCAAATGATCTCTGGAAAGCAACTTCGGTGGCTAGAGCAATGGTTACAAAGTACGGTATGAGCAAACAAGTTGGTCTCGTCAGTCATGATTACGACGACAACGGTAGTAGCATGAGCACAGAAACTAGGTTGCTCATCGAAGAAGTGAAGTACTTATTGGAAAGGGCATACAACAATGCAAAAACCATCCTCACCGAACACAACAAGGAACACCACGCCCTTGCTAATGCTTTAATAGAGAAGGAAACACTCACCGGAACTCAGATAAAGTCCTTGCTTGCACAAGTAAACTCCCAGCAGCAGCAGCAGCAACCGCACCAGCAAGTAGTTGCAGCGAAGAGCGACTCACAGTCTAAGCCAGTACCGCCCTCCACCCCCAACGCGGCTGCTTCTGCCGCAGCCGCTGCATCCGCTGCTGCAGCTGCCGCAACTGCTGCAGCCAAGTCCACAGGTATTGCACCCGTCGGATCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009536 GO:0009888 GO:0009941 GO:0010073 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048507 GO:0048856 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04139 map04139

Pfam Domains

Protein Families

AAA Peptidase_M41

Protein Analysis

717

Amino Acids

77.34

Weight (kDa)

8.71

Isoelectric Point (pI)

42.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIP49	PF06068	206 - 294	3.6e-06	TIP49 P-loop domain
AAA	PF00004	261 - 390	6.2e-46	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	413 - 456	2.1e-12	AAA+ lid domain
Peptidase_M41	PF01434	472 - 651	3e-64	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003616)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26140
fragaria_vesca	FvH4_1g07720 FvH4_2g31730 FvH4_2g31730
malus_domestica	MD08G1063300.v1.1 MD15G1058100.v1.1 MD15G1058500.v1.1
prunus_persica	Prupe.1G410800_v2.0.a1
pyrus_communis	pycom08g05200 pycom15g05470 pycom15g05500
rosa_chinensis	RchiOBHm_Chr2g0093601 RchiOBHm_Chr6g0311091
rosa_laevigata	RLG00000010436 RLG00000016420
rosa_multiflora	Rmu_co8327861.1_g000001 Rmu_co8491229.1_g000001 Rmu_sc0002126.1_g000022 Rmu_sc0008967.1_g000002 Rmu_sc0012235.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147960 Rroxscaffold_7G00158030
rosa_rugosa	Rorug02G0037800 Rorug02G0037800 Rorug06G0385300 Rorug06G0385400
rosa_samantha	Rh2AG084300 Rh2BG084900 Rh2CG087200 Rh2DG082900 Rh6AG499400 Rh6BG509900 Rh6CG515700 Rh6DG502300
rosa_wichuraiana	Rw6G043500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1242
AccB1I	GGYRCC	2 cut(s)	12, 110
AccII	CGCG	3 cut(s)	189, 1585, 2060
AccIII	TCCGGA	1 cut(s)	1280
AciI	CCGC	9 cut(s)	946, 1585, 1991, 2042, 2060, 2073, 2079, 2088, 2103
AclI	AACGTT	1 cut(s)	1308
AclWI	GGATC	4 cut(s)	731, 744, 930, 2139
AcsI	RAATTY	3 cut(s)	210, 439, 668
AcuI	CTGAAG	3 cut(s)	285, 1247, 1340
AcyI	GRCGYC	2 cut(s)	13, 1239
AfaI	GTAC	6 cut(s)	186, 541, 1019, 1730, 1829, 2040
AfiI	CCNNNNNNNGG	2 cut(s)	806, 2059
AflIII	ACRYGT	1 cut(s)	1212
AgsI	TTSAA	8 cut(s)	33, 151, 272, 644, 884, 1052, 1184, 1367
AhdI	GACNNNNNGTC	1 cut(s)	2026
AjnI	CCWGG	3 cut(s)	799, 1138, 1270
AjuI	GAANNNNNNNTTGG	4 cut(s)	453, 485, 1817, 1849
AloI	GAACNNNNNNTCC	2 cut(s)	70, 102
Alw21I	GWGCWC	7 cut(s)	139, 487, 592, 1495, 1608, 1644, 1796
Alw26I	GTCTC	4 cut(s)	1119, 1129, 1585, 1757
AlwI	GGATC	4 cut(s)	731, 744, 930, 2139
AlwNI	CAGNNNCTG	1 cut(s)	2081
Ama87I	CYCGRG	1 cut(s)	1559
Aor13HI	TCCGGA	1 cut(s)	1280
AoxI	GGCC	2 cut(s)	561, 1230
ApoI	RAATTY	3 cut(s)	210, 439, 668
ArsI	GACNNNNNNTTYG	2 cut(s)	932, 964
Asp700I	GAANNNNTTC	2 cut(s)	465, 720
AspA2I	CCTAGG	1 cut(s)	1523
AspLEI	GCGC	2 cut(s)	15, 1537
AspS9I	GGNCC	2 cut(s)	794, 1567
AsuHPI	GGTGA	5 cut(s)	928, 942, 1144, 1861, 1921
AvaI	CYCGRG	1 cut(s)	1559
AvaII	GGWCC	2 cut(s)	794, 1567
AvrII	CCTAGG	1 cut(s)	1523
BamHI	GGATCC	2 cut(s)	736, 2144
BanI	GGYRCC	2 cut(s)	12, 110
BanII	GRGCYC	3 cut(s)	910, 1608, 1644
Bbv12I	GWGCWC	7 cut(s)	139, 487, 592, 1495, 1608, 1644, 1796
BccI	CCATC	5 cut(s)	289, 1052, 1384, 1481, 1871
BceAI	ACGGC	1 cut(s)	1397
BcgI	CGANNNNNNTGC	2 cut(s)	604, 638
BciT130I	CCWGG	3 cut(s)	801, 1140, 1272
BclI	TGATCA	2 cut(s)	18, 1302
BcoDI	GTCTC	4 cut(s)	1119, 1129, 1585, 1757
BfaI	CTAG	8 cut(s)	204, 648, 758, 1260, 1524, 1710, 1803, 2149
BfmI	CTRYAG	3 cut(s)	504, 2094, 2112
BfoI	RGCGCY	2 cut(s)	16, 1538
BlnI	CCTAGG	1 cut(s)	1523
BmcAI	AGTACT	1 cut(s)	1829
Bme1390I	CCNGG	3 cut(s)	801, 1140, 1272
Bme18I	GGWCC	2 cut(s)	794, 1567
BmeRI	GACNNNNNGTC	1 cut(s)	2026
BmeT110I	CYCGRG	1 cut(s)	1559
BmgT120I	GGNCC	2 cut(s)	794, 1567
BmiI	GGNNCC	5 cut(s)	14, 112, 738, 852, 2146
BmrFI	CCNGG	3 cut(s)	801, 1140, 1272
BmsI	GCATC	4 cut(s)	46, 1584, 1685, 2093
BoxI	GACNNNNGTC	1 cut(s)	1577
BpmI	CTGGAG	2 cut(s)	858, 1254
Bpu10I	CCTNAGC	1 cut(s)	741
BpuEI	CTTGAG	2 cut(s)	123, 350
Bsa29I	ATCGAT	1 cut(s)	40
BsaHI	GRCGYC	2 cut(s)	13, 1239
BsaI	GGTCTC	2 cut(s)	1129, 1757
BsaJI	CCNNGG	3 cut(s)	1271, 1329, 1523
BsaWI	WCCGGW	3 cut(s)	1280, 1670, 1931
Bsc4I	CCNNNNNNNGG	2 cut(s)	806, 2059
Bse118I	RCCGGY	1 cut(s)	419
Bse1I	ACTGG	3 cut(s)	564, 1499, 2036
Bse3DI	GCAATG	5 cut(s)	371, 414, 831, 1089, 1722
BseAI	TCCGGA	1 cut(s)	1280
BseBI	CCWGG	3 cut(s)	801, 1140, 1272
BseCI	ATCGAT	1 cut(s)	40
BseDI	CCNNGG	3 cut(s)	1271, 1329, 1523
BseGI	GGATG	3 cut(s)	85, 1863, 2084
BseLI	CCNNNNNNNGG	2 cut(s)	806, 2059
BseMI	GCAATG	5 cut(s)	371, 414, 831, 1089, 1722
BseMII	CTCAG	5 cut(s)	38, 441, 448, 945, 1952
BseNI	ACTGG	3 cut(s)	564, 1499, 2036
BseRI	GAGGAG	2 cut(s)	120, 650
BseYI	CCCAGC	1 cut(s)	1971
Bsh1236I	CGCG	3 cut(s)	189, 1585, 2060
BshFI	GGCC	2 cut(s)	563, 1232
BshNI	GGYRCC	2 cut(s)	12, 110
BshVI	ATCGAT	1 cut(s)	40
BsiHKAI	GWGCWC	7 cut(s)	139, 487, 592, 1495, 1608, 1644, 1796
BsiHKCI	CYCGRG	1 cut(s)	1559
BsiSI	CCGG	5 cut(s)	114, 420, 1281, 1671, 1932
BslFI	GGGAC	1 cut(s)	66
BslI	CCNNNNNNNGG	2 cut(s)	806, 2059
BsmAI	GTCTC	4 cut(s)	1119, 1129, 1585, 1757
BsmFI	GGGAC	1 cut(s)	66
BsnI	GGCC	2 cut(s)	563, 1232
Bso31I	GGTCTC	2 cut(s)	1129, 1757
BsoBI	CYCGRG	1 cut(s)	1559
Bsp1286I	GDGCHC	8 cut(s)	139, 487, 592, 910, 1495, 1608, 1644, 1796
Bsp13I	TCCGGA	1 cut(s)	1280
Bsp19I	CCATGG	1 cut(s)	1329
BspACI	CCGC	9 cut(s)	946, 1585, 1991, 2042, 2060, 2073, 2079, 2088, 2103
BspANI	GGCC	2 cut(s)	563, 1232
BspCNI	CTCAG	5 cut(s)	37, 440, 447, 944, 1951
BspDI	ATCGAT	1 cut(s)	40
BspEI	TCCGGA	1 cut(s)	1280
BspFNI	CGCG	3 cut(s)	189, 1585, 2060
BspHI	TCATGA	2 cut(s)	1450, 1762
BspLI	GGNNCC	5 cut(s)	14, 112, 738, 852, 2146
BspMAI	CTGCAG	2 cut(s)	2098, 2116
BspPI	GGATC	4 cut(s)	731, 744, 930, 2139
BspQI	GCTCTTC	3 cut(s)	1500, 1632, 2009
BspT107I	GGYRCC	2 cut(s)	12, 110
BspTNI	GGTCTC	2 cut(s)	1129, 1757
BsrDI	GCAATG	5 cut(s)	371, 414, 831, 1089, 1722
BsrFI	RCCGGY	1 cut(s)	419
BsrI	ACTGG	3 cut(s)	564, 1499, 2036
BssAI	RCCGGY	1 cut(s)	419
BssECI	CCNNGG	3 cut(s)	1271, 1329, 1523
BssNI	GRCGYC	2 cut(s)	13, 1239
BssT1I	CCWWGG	2 cut(s)	1329, 1523
Bst2UI	CCWGG	3 cut(s)	801, 1140, 1272
Bst4CI	ACNGT	5 cut(s)	1146, 1337, 1733, 1781, 2028
Bst6I	CTCTTC	4 cut(s)	879, 1500, 1632, 2009
BstACI	GRCGYC	2 cut(s)	13, 1239
BstAPI	GCANNNNNTGC	3 cut(s)	2081, 2090, 2111
BstC8I	GCNNGC	5 cut(s)	344, 421, 1464, 1599, 1956
BstDEI	CTNAG	7 cut(s)	24, 427, 434, 741, 931, 1938, 2031
BstDSI	CCRYGG	1 cut(s)	1329
BstEII	GGTNACC	1 cut(s)	948
BstENI	CCTNNNNNAGG	1 cut(s)	804
BstF5I	GGATG	3 cut(s)	85, 1863, 2084
BstFNI	CGCG	3 cut(s)	189, 1585, 2060
BstH2I	RGCGCY	2 cut(s)	16, 1538
BstHHI	GCGC	2 cut(s)	15, 1537
BstMAI	GTCTC	4 cut(s)	1119, 1129, 1585, 1757
BstNI	CCWGG	3 cut(s)	801, 1140, 1272
BstNSI	RCATGY	2 cut(s)	1216, 1466
BstPAI	GACNNNNGTC	1 cut(s)	1577
BstPI	GGTNACC	1 cut(s)	948
BstSCI	CCNGG	3 cut(s)	799, 1138, 1270
BstSFI	CTRYAG	3 cut(s)	504, 2094, 2112
BstUI	CGCG	3 cut(s)	189, 1585, 2060
BstX2I	RGATCY	3 cut(s)	736, 922, 2144
BstXI	CCANNNNNNTGG	1 cut(s)	1487
BstYI	RGATCY	3 cut(s)	736, 922, 2144
Bsu15I	ATCGAT	1 cut(s)	40
BsuRI	GGCC	2 cut(s)	563, 1232
BsuTUI	ATCGAT	1 cut(s)	40
BtgI	CCRYGG	1 cut(s)	1329
BtgZI	GCGATG	1 cut(s)	690
BtsCI	GGATG	3 cut(s)	85, 1863, 2084
BtsI	GCAGTG	4 cut(s)	261, 874, 880, 1353
BtsIMutI	CAGTG	6 cut(s)	139, 261, 557, 874, 880, 1353
Cac8I	GCNNGC	5 cut(s)	344, 421, 1464, 1599, 1956
CaiI	CAGNNNCTG	1 cut(s)	2081
CciI	TCATGA	2 cut(s)	1450, 1762
CfoI	GCGC	2 cut(s)	15, 1537
Cfr10I	RCCGGY	1 cut(s)	419
Cfr13I	GGNCC	2 cut(s)	794, 1567
ClaI	ATCGAT	1 cut(s)	40
CseI	GACGC	1 cut(s)	989
Csp6I	GTAC	6 cut(s)	185, 540, 1018, 1729, 1828, 2039
CspCI	CAANNNNNGTGG	4 cut(s)	1686, 1721, 2113, 2148
CviQI	GTAC	6 cut(s)	185, 540, 1018, 1729, 1828, 2039
DdeI	CTNAG	7 cut(s)	24, 427, 434, 741, 931, 1938, 2031
DinI	GGCGCC	1 cut(s)	14
DraI	TTTAAA	2 cut(s)	523, 1066
DriI	GACNNNNNGTC	1 cut(s)	2026
Eam1104I	CTCTTC	4 cut(s)	879, 1500, 1632, 2009
Eam1105I	GACNNNNNGTC	1 cut(s)	2026
EarI	CTCTTC	4 cut(s)	879, 1500, 1632, 2009
Ecl136II	GAGCTC	2 cut(s)	1606, 1642
Eco130I	CCWWGG	2 cut(s)	1329, 1523
Eco24I	GRGCYC	3 cut(s)	910, 1608, 1644
Eco31I	GGTCTC	2 cut(s)	1129, 1757
Eco32I	GATATC	1 cut(s)	576
Eco47I	GGWCC	2 cut(s)	794, 1567
Eco53kI	GAGCTC	2 cut(s)	1606, 1642
Eco57I	CTGAAG	3 cut(s)	285, 1247, 1340
Eco88I	CYCGRG	1 cut(s)	1559
Eco91I	GGTNACC	1 cut(s)	948
EcoICRI	GAGCTC	2 cut(s)	1606, 1642
EcoNI	CCTNNNNNAGG	1 cut(s)	804
EcoO65I	GGTNACC	1 cut(s)	948
EcoRII	CCWGG	3 cut(s)	799, 1138, 1270
EcoRV	GATATC	1 cut(s)	576
EcoT14I	CCWWGG	2 cut(s)	1329, 1523
EcoT38I	GRGCYC	3 cut(s)	910, 1608, 1644
EgeI	GGCGCC	1 cut(s)	14
EheI	GGCGCC	1 cut(s)	14
ErhI	CCWWGG	2 cut(s)	1329, 1523
FalI	AAGNNNNNCTT	2 cut(s)	453, 485
FaqI	GGGAC	1 cut(s)	66
FauNDI	CATATG	1 cut(s)	495
FbaI	TGATCA	2 cut(s)	18, 1302
FokI	GGATG	3 cut(s)	72, 1850, 2071
FriOI	GRGCYC	3 cut(s)	910, 1608, 1644
FspBI	CTAG	8 cut(s)	204, 648, 758, 1260, 1524, 1710, 1803, 2149
GlaI	GCGC	2 cut(s)	14, 1536
GsaI	CCCAGC	1 cut(s)	1975
GsuI	CTGGAG	2 cut(s)	858, 1254
HaeII	RGCGCY	2 cut(s)	16, 1538
HaeIII	GGCC	2 cut(s)	563, 1232
HapII	CCGG	5 cut(s)	114, 420, 1281, 1671, 1932
HgaI	GACGC	1 cut(s)	989
HhaI	GCGC	2 cut(s)	15, 1537
Hin1I	GRCGYC	2 cut(s)	13, 1239
Hin6I	GCGC	2 cut(s)	13, 1535
HinP1I	GCGC	2 cut(s)	13, 1535
HincII	GTYRAC	1 cut(s)	278
HindII	GTYRAC	1 cut(s)	278
HinfI	GANTC	5 cut(s)	621, 656, 1112, 1427, 2021
HpaII	CCGG	5 cut(s)	114, 420, 1281, 1671, 1932
HphI	GGTGA	5 cut(s)	928, 942, 1144, 1861, 1921
Hpy166II	GTNNAC	6 cut(s)	278, 724, 797, 1504, 1966, 2124
Hpy8I	GTNNAC	6 cut(s)	278, 724, 797, 1504, 1966, 2124
Hpy99I	CGWCG	4 cut(s)	1244, 1613, 1775, 2144
HpyCH4III	ACNGT	5 cut(s)	1146, 1337, 1733, 1781, 2028
HpyCH4IV	ACGT	4 cut(s)	1179, 1239, 1308, 1611
HpyF3I	CTNAG	7 cut(s)	24, 427, 434, 741, 931, 1938, 2031
HpySE526I	ACGT	4 cut(s)	1179, 1239, 1308, 1611
Hsp92I	GRCGYC	2 cut(s)	13, 1239
HspAI	GCGC	2 cut(s)	13, 1535
KasI	GGCGCC	1 cut(s)	12
Kpn2I	TCCGGA	1 cut(s)	1280
KroI	GCCGGC	1 cut(s)	419
KroNI	GCCGGC	1 cut(s)	421
Ksp22I	TGATCA	2 cut(s)	18, 1302
LguI	GCTCTTC	3 cut(s)	1500, 1632, 2009
LmnI	GCTCC	4 cut(s)	490, 587, 774, 1283
LweI	GCATC	4 cut(s)	46, 1584, 1685, 2093
MaeI	CTAG	8 cut(s)	204, 648, 758, 1260, 1524, 1710, 1803, 2149
MaeII	ACGT	4 cut(s)	1179, 1239, 1308, 1611
MaeIII	GTNAC	5 cut(s)	360, 948, 1041, 1663, 1720
MboII	GAAGA	7 cut(s)	419, 722, 896, 1487, 1649, 1829, 2026
MflI	RGATCY	3 cut(s)	736, 922, 2144
MhlI	GDGCHC	8 cut(s)	139, 487, 592, 910, 1495, 1608, 1644, 1796
MluCI	AATT	7 cut(s)	169, 210, 439, 543, 668, 717, 1102
Mly113I	GGCGCC	1 cut(s)	13
MlyI	GAGTC	4 cut(s)	615, 1121, 1436, 2015
MmeI	TCCRAC	2 cut(s)	113, 2122
MroI	TCCGGA	1 cut(s)	1280
MroNI	GCCGGC	1 cut(s)	419
MroXI	GAANNNNTTC	2 cut(s)	465, 720
MseI	TTAA	5 cut(s)	522, 681, 1035, 1065, 1910
MslI	CAYNNNNRTG	3 cut(s)	1025, 1455, 1485
MspA1I	CMGCKG	5 cut(s)	532, 935, 2081, 2090, 2099
MspI	CCGG	5 cut(s)	114, 420, 1281, 1671, 1932
MspR9I	CCNGG	3 cut(s)	801, 1140, 1272
MvaI	CCWGG	3 cut(s)	801, 1140, 1272
MvnI	CGCG	3 cut(s)	189, 1585, 2060
NaeI	GCCGGC	1 cut(s)	421
NarI	GGCGCC	1 cut(s)	13
NcoI	CCATGG	1 cut(s)	1329
NdeI	CATATG	1 cut(s)	495
NgoMIV	GCCGGC	1 cut(s)	419
NlaIV	GGNNCC	5 cut(s)	14, 112, 738, 852, 2146
NmuCI	GTSAC	2 cut(s)	948, 1663
NspI	RCATGY	2 cut(s)	1216, 1466
PaeI	GCATGC	1 cut(s)	1466
PagI	TCATGA	2 cut(s)	1450, 1762
PciI	ACATGT	1 cut(s)	1212
PciSI	GCTCTTC	3 cut(s)	1500, 1632, 2009
PdiI	GCCGGC	1 cut(s)	421
PdmI	GAANNNNTTC	2 cut(s)	465, 720
PfeI	GAWTC	1 cut(s)	656
PleI	GAGTC	4 cut(s)	615, 1120, 1435, 2015
PluTI	GGCGCC	1 cut(s)	16
PpsI	GAGTC	4 cut(s)	615, 1120, 1435, 2015
PscI	ACATGT	1 cut(s)	1212
PshAI	GACNNNNGTC	1 cut(s)	1577
Psp124BI	GAGCTC	2 cut(s)	1608, 1644
Psp1406I	AACGTT	1 cut(s)	1308
Psp6I	CCWGG	3 cut(s)	799, 1138, 1270
PspEI	GGTNACC	1 cut(s)	948
PspFI	CCCAGC	1 cut(s)	1971
PspGI	CCWGG	3 cut(s)	799, 1138, 1270
PspN4I	GGNNCC	5 cut(s)	14, 112, 738, 852, 2146
PspPI	GGNCC	2 cut(s)	794, 1567
PstI	CTGCAG	2 cut(s)	2098, 2116
PstNI	CAGNNNCTG	1 cut(s)	2081
PsuI	RGATCY	3 cut(s)	736, 922, 2144
PvuII	CAGCTG	3 cut(s)	532, 935, 2099
RsaI	GTAC	6 cut(s)	186, 541, 1019, 1730, 1829, 2040
RsaNI	GTAC	6 cut(s)	185, 540, 1018, 1729, 1828, 2039
RseI	CAYNNNNRTG	3 cut(s)	1025, 1455, 1485
SacI	GAGCTC	2 cut(s)	1608, 1644
SapI	GCTCTTC	3 cut(s)	1500, 1632, 2009
SaqAI	TTAA	5 cut(s)	522, 681, 1035, 1065, 1910
Sau96I	GGNCC	2 cut(s)	794, 1567
ScaI	AGTACT	1 cut(s)	1829
SchI	GAGTC	4 cut(s)	615, 1121, 1436, 2015
ScrFI	CCNGG	3 cut(s)	801, 1140, 1272
SduI	GDGCHC	8 cut(s)	139, 487, 592, 910, 1495, 1608, 1644, 1796
SfaNI	GCATC	4 cut(s)	46, 1584, 1685, 2093
SfcI	CTRYAG	3 cut(s)	504, 2094, 2112
SfoI	GGCGCC	1 cut(s)	14
SinI	GGWCC	2 cut(s)	794, 1567
SmiMI	CAYNNNNRTG	3 cut(s)	1025, 1455, 1485
SmlI	CTYRAG	2 cut(s)	138, 365
SmoI	CTYRAG	2 cut(s)	138, 365
SphI	GCATGC	1 cut(s)	1466
Sse9I	AATT	7 cut(s)	169, 210, 439, 543, 668, 717, 1102
SsiI	CCGC	9 cut(s)	946, 1585, 1991, 2042, 2060, 2073, 2079, 2088, 2103
SspDI	GGCGCC	1 cut(s)	12
SspI	AATATT	1 cut(s)	963
SspMI	CTAG	8 cut(s)	204, 648, 758, 1260, 1524, 1710, 1803, 2149
SstI	GAGCTC	2 cut(s)	1608, 1644
StyD4I	CCNGG	3 cut(s)	799, 1138, 1270
StyI	CCWWGG	2 cut(s)	1329, 1523
TaaI	ACNGT	5 cut(s)	1146, 1337, 1733, 1781, 2028
TaiI	ACGT	4 cut(s)	1182, 1242, 1311, 1614
TaqI	TCGA	7 cut(s)	40, 155, 910, 1217, 1242, 1608, 1815
TasI	AATT	7 cut(s)	169, 210, 439, 543, 668, 717, 1102
TatI	WGTACW	2 cut(s)	1017, 1827
TauI	GCSGC	4 cut(s)	2063, 2075, 2081, 2105
TfiI	GAWTC	1 cut(s)	656
Tru1I	TTAA	5 cut(s)	522, 681, 1035, 1065, 1910
Tru9I	TTAA	5 cut(s)	522, 681, 1035, 1065, 1910
TscAI	CASTG	6 cut(s)	139, 261, 564, 874, 880, 1353
TseFI	GTSAC	2 cut(s)	948, 1663
Tsp45I	GTSAC	2 cut(s)	948, 1663
TspDTI	ATGAA	8 cut(s)	213, 264, 482, 852, 955, 1037, 1089, 1439
TspRI	CASTG	6 cut(s)	139, 261, 564, 874, 880, 1353
VpaK11BI	GGWCC	2 cut(s)	794, 1567
XagI	CCTNNNNNAGG	1 cut(s)	804
XapI	RAATTY	3 cut(s)	210, 439, 668
XceI	RCATGY	2 cut(s)	1216, 1466
XmaJI	CCTAGG	1 cut(s)	1523
XmnI	GAANNNNTTC	2 cut(s)	465, 720
XspI	CTAG	8 cut(s)	204, 648, 758, 1260, 1524, 1710, 1803, 2149
ZraI	GACGTC	1 cut(s)	1240
ZrmI	AGTACT	1 cut(s)	1829

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.