Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0003616

Physical Location & Seq

Genomic Coordinates Reverse (-)

67319044 .. 67323832

Gene Structure 4789 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG515700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2151 bp

ATGGCCCTGAGACGCCTGCTCACTCAGGTATCTAAGCATCGATCAGAGTTGGGACAGCTGAAACAAGTTGTTTCTAGAACATATTTTCCGGTCAACAGGGTTGCAGGAGGTGCTGGAGGGAATACAATTTTTAATACTCAAGAGAGGTGTCAGTCAAGCTATGTGCGCAATTTGGCTCGCAGAGGACGTGATGTTGATGAAGCTAATGAACTTGCCTATCTAAAAGAACTGTCCATGCGAAATGACCCTGAAGAAGTTATAAGATTATTTGAAAGTCAACCATCATTGCATTCCAACTCTTCAGCGCTTTCAGAATATGTGAAAGCATTGGTAAAAGTTGGCAGGCTGGATGAGAGTGAGTTACTGAAGACATTGCAGAGAGGTGTATCCAACTCTGCAAGGGAGGATGAAAGCATGGGTGGCTTCTCAGTTCTTAGGAACGTTGGGAAGTCTACAAAAGATGGCGTTCTTGGAACTGCAAGTGCACCTATTCATATGGTGGCTAGAGAAGGTGGTCATTTTAAAGAGCAGCTGTGGCGTACGGTTCGCACAATTGCACTAGCCTTTCTTTTGATATCTGGCGTTGGAGCACTCATTGAGGATAGAGGAATAAGCAAAGGACTTGGATTAAATGAAGAGGTCCAACCTAGCATGGAATCTAGCACTAAGTTTAGCGATGTCAAAGGAGTTGATGAGGCTAAGGCGGAGCTAGAAGAAATTGTTCACTATCTTCGAGATCCCAAGCGTTTCACTCGTCTTGGTGGCAAGCTACCAAAGGGTGTTTTGCTTGTTGGCCCACCTGGCACTGGAAAGACAATGTTAGCAAGAGCAATTGCCGGAGAAGCAGGGGTACCTTTCTTTTCATGCAGTGGCAGTGAATTTGAAGAGATGTTTGTTGGCGTTGGAGCTCGAAGGGTGAGGGATCTCTTTGCAGCTGCAAAGAAGCGGTCACCGTGTATTATATTTATTGATGAGATAGATGCTATAGGAGGAAGTCGCAACCCAAAGGATCAGCAGTACATGAAGATGACTCTGAATCAGTTACTTGTTGAACTTGATGGTTTTAAACAAAATGAAGGAATTATTGTTATTGCCGCTACTAATTTCCCCGAGTCACTGGACAAGGCATTGGTGAGACCTGGACGGTTTGATCGCCACATTGTTGTTCCCAATCCTGATGTTGAAGGGCGGAGGCAGATTATGGAAGTTCACATGTCTAAGGTTATGAAGGCTGATGATGTTGATTTGATGATCATTGCTAGAGGAACTCCTGGGTTCTCCGGTGCTGATCTGGCCAACTTGATCAACGTAGCTGCTCTTAAGGCTGCCATGGATGGCGCTAAAGCAGTGAGTATGGCTGATCTGGAGTATGCAAAAGACAAGATTATGATGGGAAGTGAGCGAAAGTCAGCTGTCATATCTGATGAGTCACGAAAGCTAACAGCTTTTCATGAGGGTGGCCATGCACTTGTGGCTGTTCACACTGATGGTGCCCATCCAGTTCACAAGGCAACAATAGTTCCCCGTGGTATGGCACTTGGCATGGTTGCCCAGTTGCCCGAAAAGGATGAGACAAGTGTCTCACGGAAACAGATGCTTGCGCGGCTTGATGTTTGCATGGGGGGACGGGTTGCAGAAGAGCTCATCTTTGGTGAAAGTGAAGTAACTTCTGGTGCTTCTGACGATCTCCGGCAAGCAACTGCTCTTGCCAGAGCAATGGTTACAAAGTATGGTATGAGCAAAGAAGTGGGGCTTGTCACTCACAATTATGACGACAATGGGAAGAGCATGAGCACAGAAACTAGGCTGCTTATTGAAGAAGAAGTGAAGCACCTCTTGGAAACGGCTTACAACAATGCAAAGAATATACTTACCACACATCACAAGGAGCTCCATGCTCTTGCTAATGCCTTATTGGAGCAAGAGACACTCACCGGAAAACAGATCAAGGTCTTGCTTGCTAACGTAAACTCTCAGCAGAACCAACAACCGCAACAGCAAGTTGTTGCAGAAACGTCACAGTCAAAACCGGTGCCGCCTCCATCACCACCCAATGCAGCTCAGTCTGCTGCAGCAGCAGCAGCAGCCGCCGCGGCCGCCGCTGCAACTGCTGCAGCCAAATCAAAAGGCATGGCTCCTGTGGGATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009536 GO:0009888 GO:0009941 GO:0010073 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048507 GO:0048856 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04139 map04139

Pfam Domains

Protein Families

AAA Peptidase_M41

Protein Analysis

716

Amino Acids

77.27

Weight (kDa)

8.39

Isoelectric Point (pI)

43.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIP49	PF06068	206 - 294	5.5e-06	TIP49 P-loop domain
AAA_5	PF07728	260 - 330	4.3e-06	AAA domain (dynein-related subfamily)
AAA	PF00004	261 - 390	3.8e-46	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	413 - 456	5.4e-12	AAA+ lid domain
Peptidase_M41	PF01434	472 - 650	2.9e-67	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003616)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26140
fragaria_vesca	FvH4_1g07720 FvH4_2g31730 FvH4_2g31730
malus_domestica	MD08G1063300.v1.1 MD15G1058100.v1.1 MD15G1058500.v1.1
prunus_persica	Prupe.1G410800_v2.0.a1
pyrus_communis	pycom08g05200 pycom15g05470 pycom15g05500
rosa_chinensis	RchiOBHm_Chr2g0093601 RchiOBHm_Chr6g0311091
rosa_laevigata	RLG00000010436 RLG00000016420
rosa_multiflora	Rmu_co8327861.1_g000001 Rmu_co8491229.1_g000001 Rmu_sc0002126.1_g000022 Rmu_sc0008967.1_g000002 Rmu_sc0012235.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147960 Rroxscaffold_7G00158030
rosa_rugosa	Rorug02G0037800 Rorug02G0037800 Rorug06G0385300 Rorug06G0385400
rosa_samantha	Rh2AG084300 Rh2BG084900 Rh2CG087200 Rh2DG082900 Rh6AG499400 Rh6BG509900 Rh6CG515700 Rh6DG502300
rosa_wichuraiana	Rw6G043500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	260
Acc16I	TGCGCA	1 cut(s)	167
Acc65I	GGTACC	1 cut(s)	850
AccB1I	GGYRCC	3 cut(s)	850, 1490, 2032
AccI	GTMKAC	1 cut(s)	452
AccII	CGCG	2 cut(s)	1603, 2093
AclI	AACGTT	1 cut(s)	441
AclWI	GGATC	3 cut(s)	731, 930, 1017
AcoI	YGGCCR	3 cut(s)	1293, 1459, 2094
AcsI	RAATTY	1 cut(s)	878
AcuI	CTGAAG	3 cut(s)	270, 285, 386
AcyI	GRCGYC	1 cut(s)	13
AfaI	GTAC	3 cut(s)	541, 852, 1019
AfeI	AGCGCT	1 cut(s)	306
AfiI	CCNNNNNNNGG	2 cut(s)	806, 1531
AflII	CTTAAG	1 cut(s)	1319
AflIII	ACRYGT	1 cut(s)	1212
AgeI	ACCGGT	1 cut(s)	2029
AgsI	TTSAA	5 cut(s)	272, 884, 1052, 1184, 1817
AjiI	CACGTC	1 cut(s)	188
AjnI	CCWGG	3 cut(s)	799, 1138, 1270
AjuI	GAANNNNNNNTTGG	2 cut(s)	1820, 1852
AloI	GAACNNNNNNTCC	2 cut(s)	70, 102
Alw21I	GWGCWC	6 cut(s)	487, 592, 910, 1644, 1796, 1893
Alw26I	GTCTC	5 cut(s)	4, 1129, 1565, 1585, 1919
Alw44I	GTGCAC	1 cut(s)	483
AlwI	GGATC	3 cut(s)	731, 930, 1017
Ama87I	CYCGRG	1 cut(s)	1109
Aor51HI	AGCGCT	1 cut(s)	306
AoxI	GGCC	5 cut(s)	3, 793, 1293, 1459, 2094
ApaLI	GTGCAC	1 cut(s)	483
ApoI	RAATTY	1 cut(s)	878
ArsI	GACNNNNNNTTYG	2 cut(s)	932, 964
AsiGI	ACCGGT	1 cut(s)	2029
Asp700I	GAANNNNTTC	1 cut(s)	720
Asp718I	GGTACC	1 cut(s)	850
AspLEI	GCGC	4 cut(s)	168, 307, 1340, 1603
AspS9I	GGNCC	3 cut(s)	4, 640, 794
AsuHPI	GGTGA	6 cut(s)	928, 942, 1144, 1664, 1924, 2037
AvaI	CYCGRG	1 cut(s)	1109
AvaII	GGWCC	1 cut(s)	640
BaeGI	GKGCMC	2 cut(s)	487, 1495
BaeI	ACNNNNGTAYC	2 cut(s)	12, 45
BalI	TGGCCA	2 cut(s)	1295, 1461
BanI	GGYRCC	3 cut(s)	850, 1490, 2032
BanII	GRGCYC	3 cut(s)	910, 1644, 1893
BarI	GAAGNNNNNNTAC	2 cut(s)	834, 866
BbsI	GAAGAC	1 cut(s)	374
Bbv12I	GWGCWC	6 cut(s)	487, 592, 910, 1644, 1796, 1893
BccI	CCATC	8 cut(s)	289, 455, 1052, 1328, 1384, 1481, 1503, 2050
BceAI	ACGGC	1 cut(s)	1860
BciT130I	CCWGG	3 cut(s)	801, 1140, 1272
BciVI	GTATCC	1 cut(s)	397
BclI	TGATCA	2 cut(s)	1251, 1302
BcoDI	GTCTC	5 cut(s)	4, 1129, 1565, 1585, 1919
BfaI	CTAG	8 cut(s)	75, 504, 560, 648, 660, 710, 1260, 1803
BfmI	CTRYAG	3 cut(s)	984, 2070, 2112
BfoI	RGCGCY	2 cut(s)	308, 1341
BfrI	CTTAAG	1 cut(s)	1319
BfuI	GTATCC	1 cut(s)	397
BglI	GCCNNNNNGGC	2 cut(s)	801, 2093
Bme1390I	CCNGG	3 cut(s)	801, 1140, 1272
Bme18I	GGWCC	1 cut(s)	640
BmeT110I	CYCGRG	1 cut(s)	1109
BmgBI	CACGTC	1 cut(s)	188
BmgT120I	GGNCC	3 cut(s)	4, 640, 794
BmiI	GGNNCC	4 cut(s)	852, 1492, 2034, 2136
BmrFI	CCNGG	3 cut(s)	801, 1140, 1272
BmrI	ACTGGG	1 cut(s)	1546
BmsI	GCATC	3 cut(s)	46, 970, 1584
BmuI	ACTGGG	1 cut(s)	1546
BoxI	GACNNNNGTC	1 cut(s)	1577
BpiI	GAAGAC	1 cut(s)	374
BpmI	CTGGAG	2 cut(s)	135, 1385
Bpu10I	CCTNAGC	1 cut(s)	699
BpuEI	CTTGAG	1 cut(s)	123
Bsa29I	ATCGAT	1 cut(s)	40
BsaHI	GRCGYC	1 cut(s)	13
BsaI	GGTCTC	1 cut(s)	1129
BsaJI	CCNNGG	4 cut(s)	1271, 1329, 1525, 2091
BsaWI	WCCGGW	4 cut(s)	88, 1280, 1934, 2029
Bsc4I	CCNNNNNNNGG	2 cut(s)	806, 1531
Bse118I	RCCGGY	1 cut(s)	2029
Bse1I	ACTGG	4 cut(s)	811, 1122, 1499, 1552
Bse3DI	GCAATG	4 cut(s)	284, 371, 1254, 1722
BseBI	CCWGG	3 cut(s)	801, 1140, 1272
BseCI	ATCGAT	1 cut(s)	40
BseDI	CCNNGG	4 cut(s)	1271, 1329, 1525, 2091
BseGI	GGATG	5 cut(s)	355, 412, 1339, 1495, 1573
BseLI	CCNNNNNNNGG	2 cut(s)	806, 1531
BseMI	GCAATG	4 cut(s)	284, 371, 1254, 1722
BseMII	CTCAG	4 cut(s)	38, 441, 1988, 2075
BseNI	ACTGG	4 cut(s)	811, 1122, 1499, 1552
BseSI	GKGCMC	2 cut(s)	487, 1495
BseX3I	CGGCCG	1 cut(s)	2094
Bsh1236I	CGCG	2 cut(s)	1603, 2093
Bsh1285I	CGRYCG	1 cut(s)	2097
BshFI	GGCC	5 cut(s)	5, 795, 1295, 1461, 2096
BshNI	GGYRCC	3 cut(s)	850, 1490, 2032
BshTI	ACCGGT	1 cut(s)	2029
BshVI	ATCGAT	1 cut(s)	40
BsiEI	CGRYCG	1 cut(s)	2097
BsiHKAI	GWGCWC	6 cut(s)	487, 592, 910, 1644, 1796, 1893
BsiHKCI	CYCGRG	1 cut(s)	1109
BsiSI	CCGG	6 cut(s)	89, 837, 1281, 1690, 1935, 2030
BsiWI	CGTACG	1 cut(s)	539
BslFI	GGGAC	2 cut(s)	66, 1638
BslI	CCNNNNNNNGG	2 cut(s)	806, 1531
BsmAI	GTCTC	5 cut(s)	4, 1129, 1565, 1585, 1919
BsmBI	CGTCTC	1 cut(s)	4
BsmFI	GGGAC	2 cut(s)	66, 1638
BsmI	GAATGC	1 cut(s)	289
BsnI	GGCC	5 cut(s)	5, 795, 1295, 1461, 2096
Bso31I	GGTCTC	1 cut(s)	1129
BsoBI	CYCGRG	1 cut(s)	1109
Bsp1286I	GDGCHC	7 cut(s)	487, 592, 910, 1495, 1644, 1796, 1893
Bsp19I	CCATGG	1 cut(s)	1329
BspANI	GGCC	5 cut(s)	5, 795, 1295, 1461, 2096
BspCNI	CTCAG	4 cut(s)	37, 440, 1987, 2074
BspDI	ATCGAT	1 cut(s)	40
BspFNI	CGCG	2 cut(s)	1603, 2093
BspHI	TCATGA	1 cut(s)	1450
BspLI	GGNNCC	4 cut(s)	852, 1492, 2034, 2136
BspMAI	CTGCAG	2 cut(s)	2074, 2116
BspPI	GGATC	3 cut(s)	731, 930, 1017
BspQI	GCTCTTC	2 cut(s)	1632, 1778
BspT107I	GGYRCC	3 cut(s)	850, 1490, 2032
BspTI	CTTAAG	1 cut(s)	1319
BspTNI	GGTCTC	1 cut(s)	1129
BsrDI	GCAATG	4 cut(s)	284, 371, 1254, 1722
BsrFI	RCCGGY	1 cut(s)	2029
BsrI	ACTGG	4 cut(s)	811, 1122, 1499, 1552
BssAI	RCCGGY	1 cut(s)	2029
BssECI	CCNNGG	4 cut(s)	1271, 1329, 1525, 2091
BssNI	GRCGYC	1 cut(s)	13
BssT1I	CCWWGG	1 cut(s)	1329
Bst2UI	CCWGG	3 cut(s)	801, 1140, 1272
Bst4CI	ACNGT	5 cut(s)	231, 544, 954, 1146, 2022
Bst6I	CTCTTC	5 cut(s)	304, 630, 879, 1632, 1778
BstACI	GRCGYC	1 cut(s)	13
BstAFI	CTTAAG	1 cut(s)	1319
BstAPI	GCANNNNNTGC	2 cut(s)	110, 2111
BstC8I	GCNNGC	7 cut(s)	17, 178, 344, 767, 1599, 1695, 1959
BstDSI	CCRYGG	3 cut(s)	1329, 1525, 2091
BstEII	GGTNACC	1 cut(s)	948
BstF5I	GGATG	5 cut(s)	355, 412, 1339, 1495, 1573
BstFNI	CGCG	2 cut(s)	1603, 2093
BstH2I	RGCGCY	2 cut(s)	308, 1341
BstHHI	GCGC	4 cut(s)	168, 307, 1340, 1603
BstMAI	GTCTC	5 cut(s)	4, 1129, 1565, 1585, 1919
BstMCI	CGRYCG	1 cut(s)	2097
BstNI	CCWGG	3 cut(s)	801, 1140, 1272
BstNSI	RCATGY	1 cut(s)	1216
BstPAI	GACNNNNGTC	1 cut(s)	1577
BstPI	GGTNACC	1 cut(s)	948
BstSCI	CCNGG	3 cut(s)	799, 1138, 1270
BstSFI	CTRYAG	3 cut(s)	984, 2070, 2112
BstSLI	GKGCMC	2 cut(s)	487, 1495
BstUI	CGCG	2 cut(s)	1603, 2093
BstV2I	GAAGAC	1 cut(s)	374
BstX2I	RGATCY	3 cut(s)	736, 922, 2144
BstXI	CCANNNNNNTGG	1 cut(s)	1717
BstYI	RGATCY	3 cut(s)	736, 922, 2144
BstZI	CGGCCG	1 cut(s)	2094
Bsu15I	ATCGAT	1 cut(s)	40
BsuI	GTATCC	1 cut(s)	397
BsuRI	GGCC	5 cut(s)	5, 795, 1295, 1461, 2096
BsuTUI	ATCGAT	1 cut(s)	40
BtgI	CCRYGG	3 cut(s)	1329, 1525, 2091
BtgZI	GCGATG	1 cut(s)	690
BtrI	CACGTC	1 cut(s)	188
BtsCI	GGATG	5 cut(s)	355, 412, 1339, 1495, 1573
BtsI	GCAGTG	3 cut(s)	874, 880, 1353
BtsIMutI	CAGTG	6 cut(s)	804, 874, 880, 1115, 1353, 1482
Cac8I	GCNNGC	7 cut(s)	17, 178, 344, 767, 1599, 1695, 1959
CciI	TCATGA	1 cut(s)	1450
CciNI	GCGGCCGC	1 cut(s)	2094
CfoI	GCGC	4 cut(s)	168, 307, 1340, 1603
Cfr10I	RCCGGY	1 cut(s)	2029
Cfr13I	GGNCC	3 cut(s)	4, 640, 794
Cfr42I	CCGCGG	1 cut(s)	2094
ClaI	ATCGAT	1 cut(s)	40
CseI	GACGC	1 cut(s)	21
Csp6I	GTAC	3 cut(s)	540, 851, 1018
CspAI	ACCGGT	1 cut(s)	2029
CviQI	GTAC	3 cut(s)	540, 851, 1018
DraI	TTTAAA	2 cut(s)	523, 1066
EaeI	YGGCCR	3 cut(s)	1293, 1459, 2094
EagI	CGGCCG	1 cut(s)	2094
Eam1104I	CTCTTC	5 cut(s)	304, 630, 879, 1632, 1778
EarI	CTCTTC	5 cut(s)	304, 630, 879, 1632, 1778
EciI	GGCGGA	2 cut(s)	719, 1204
Ecl136II	GAGCTC	3 cut(s)	908, 1642, 1891
EclXI	CGGCCG	1 cut(s)	2094
Eco130I	CCWWGG	1 cut(s)	1329
Eco24I	GRGCYC	3 cut(s)	910, 1644, 1893
Eco31I	GGTCTC	1 cut(s)	1129
Eco32I	GATATC	1 cut(s)	576
Eco47I	GGWCC	1 cut(s)	640
Eco47III	AGCGCT	1 cut(s)	306
Eco52I	CGGCCG	1 cut(s)	2094
Eco53kI	GAGCTC	3 cut(s)	908, 1642, 1891
Eco57I	CTGAAG	3 cut(s)	270, 285, 386
Eco88I	CYCGRG	1 cut(s)	1109
Eco91I	GGTNACC	1 cut(s)	948
EcoICRI	GAGCTC	3 cut(s)	908, 1642, 1891
EcoO65I	GGTNACC	1 cut(s)	948
EcoRII	CCWGG	3 cut(s)	799, 1138, 1270
EcoRV	GATATC	1 cut(s)	576
EcoT14I	CCWWGG	1 cut(s)	1329
EcoT38I	GRGCYC	3 cut(s)	910, 1644, 1893
ErhI	CCWWGG	1 cut(s)	1329
Esp3I	CGTCTC	1 cut(s)	4
FalI	AAGNNNNNCTT	4 cut(s)	1737, 1769, 1820, 1852
FaqI	GGGAC	2 cut(s)	66, 1638
FauNDI	CATATG	1 cut(s)	495
FbaI	TGATCA	2 cut(s)	1251, 1302
FblI	GTMKAC	1 cut(s)	452
FokI	GGATG	5 cut(s)	362, 419, 1346, 1482, 1580
FriOI	GRGCYC	3 cut(s)	910, 1644, 1893
FspBI	CTAG	8 cut(s)	75, 504, 560, 648, 660, 710, 1260, 1803
FspI	TGCGCA	1 cut(s)	167
GlaI	GCGC	4 cut(s)	167, 306, 1339, 1602
GsuI	CTGGAG	2 cut(s)	135, 1385
HaeII	RGCGCY	2 cut(s)	308, 1341
HaeIII	GGCC	5 cut(s)	5, 795, 1295, 1461, 2096
HapII	CCGG	6 cut(s)	89, 837, 1281, 1690, 1935, 2030
HgaI	GACGC	1 cut(s)	21
HhaI	GCGC	4 cut(s)	168, 307, 1340, 1603
Hin1I	GRCGYC	1 cut(s)	13
Hin6I	GCGC	4 cut(s)	166, 305, 1338, 1601
HinP1I	GCGC	4 cut(s)	166, 305, 1338, 1601
HincII	GTYRAC	2 cut(s)	94, 278
HindII	GTYRAC	2 cut(s)	94, 278
HinfI	GANTC	5 cut(s)	656, 1030, 1036, 1112, 1427
HpaII	CCGG	6 cut(s)	89, 837, 1281, 1690, 1935, 2030
HphI	GGTGA	6 cut(s)	928, 942, 1144, 1664, 1924, 2037
Hpy166II	GTNNAC	9 cut(s)	94, 278, 453, 485, 724, 1210, 1480, 1504, 1969
Hpy188I	TCNGA	5 cut(s)	46, 313, 1035, 1423, 1681
Hpy188III	TCNNGA	7 cut(s)	75, 140, 734, 1175, 1364, 1431, 1451
Hpy8I	GTNNAC	9 cut(s)	94, 278, 453, 485, 724, 1210, 1480, 1504, 1969
HpyAV	CCTTC	5 cut(s)	503, 906, 1070, 1178, 1222
HpyCH4III	ACNGT	5 cut(s)	231, 544, 954, 1146, 2022
HpyCH4IV	ACGT	5 cut(s)	187, 441, 1308, 1965, 2015
HpySE526I	ACGT	5 cut(s)	187, 441, 1308, 1965, 2015
Hsp92I	GRCGYC	1 cut(s)	13
HspAI	GCGC	4 cut(s)	166, 305, 1338, 1601
KpnI	GGTACC	1 cut(s)	854
Ksp22I	TGATCA	2 cut(s)	1251, 1302
KspI	CCGCGG	1 cut(s)	2094
LguI	GCTCTTC	2 cut(s)	1632, 1778
LmnI	GCTCC	7 cut(s)	587, 706, 905, 1888, 1896, 1918, 2140
LweI	GCATC	3 cut(s)	46, 970, 1584
MaeI	CTAG	8 cut(s)	75, 504, 560, 648, 660, 710, 1260, 1803
MaeII	ACGT	5 cut(s)	187, 441, 1308, 1965, 2015
MaeIII	GTNAC	9 cut(s)	360, 948, 1041, 1113, 1428, 1663, 1720, 1756, 2016
MfeI	CAATTG	2 cut(s)	552, 831
MflI	RGATCY	3 cut(s)	736, 922, 2144
MhlI	GDGCHC	7 cut(s)	487, 592, 910, 1495, 1644, 1796, 1893
MlsI	TGGCCA	2 cut(s)	1295, 1461
MluCI	AATT	9 cut(s)	126, 169, 552, 717, 831, 878, 1080, 1102, 1765
MluNI	TGGCCA	2 cut(s)	1295, 1461
MlyI	GAGTC	3 cut(s)	1024, 1121, 1436
MmeI	TCCRAC	5 cut(s)	318, 414, 565, 667, 883
Mox20I	TGGCCA	2 cut(s)	1295, 1461
MroXI	GAANNNNTTC	1 cut(s)	720
MscI	TGGCCA	2 cut(s)	1295, 1461
MseI	TTAA	5 cut(s)	132, 522, 629, 1065, 1320
MslI	CAYNNNNRTG	4 cut(s)	1025, 1455, 1485, 1767
Msp20I	TGGCCA	2 cut(s)	1295, 1461
MspA1I	CMGCKG	6 cut(s)	58, 532, 935, 1412, 2093, 2102
MspCI	CTTAAG	1 cut(s)	1319
MspI	CCGG	6 cut(s)	89, 837, 1281, 1690, 1935, 2030
MspR9I	CCNGG	3 cut(s)	801, 1140, 1272
MunI	CAATTG	2 cut(s)	552, 831
Mva1269I	GAATGC	1 cut(s)	289
MvaI	CCWGG	3 cut(s)	801, 1140, 1272
MvnI	CGCG	2 cut(s)	1603, 2093
NcoI	CCATGG	1 cut(s)	1329
NdeI	CATATG	1 cut(s)	495
NlaIV	GGNNCC	4 cut(s)	852, 1492, 2034, 2136
NmuCI	GTSAC	5 cut(s)	948, 1113, 1428, 1756, 2016
NotI	GCGGCCGC	1 cut(s)	2094
NsbI	TGCGCA	1 cut(s)	167
NspI	RCATGY	1 cut(s)	1216
PagI	TCATGA	1 cut(s)	1450
PciI	ACATGT	1 cut(s)	1212
PciSI	GCTCTTC	2 cut(s)	1632, 1778
PcsI	WCGNNNNNNNCGW	1 cut(s)	184
PctI	GAATGC	1 cut(s)	289
PdmI	GAANNNNTTC	1 cut(s)	720
PfeI	GAWTC	2 cut(s)	656, 1036
Pfl23II	CGTACG	1 cut(s)	539
PinAI	ACCGGT	1 cut(s)	2029
PleI	GAGTC	3 cut(s)	1024, 1120, 1435
PpsI	GAGTC	3 cut(s)	1024, 1120, 1435
PscI	ACATGT	1 cut(s)	1212
PshAI	GACNNNNGTC	1 cut(s)	1577
PsiI	TTATAA	1 cut(s)	260
Psp124BI	GAGCTC	3 cut(s)	910, 1644, 1893
Psp1406I	AACGTT	1 cut(s)	441
Psp6I	CCWGG	3 cut(s)	799, 1138, 1270
PspEI	GGTNACC	1 cut(s)	948
PspGI	CCWGG	3 cut(s)	799, 1138, 1270
PspLI	CGTACG	1 cut(s)	539
PspN4I	GGNNCC	4 cut(s)	852, 1492, 2034, 2136
PspPI	GGNCC	3 cut(s)	4, 640, 794
PstI	CTGCAG	2 cut(s)	2074, 2116
PsuI	RGATCY	3 cut(s)	736, 922, 2144
PvuII	CAGCTG	4 cut(s)	58, 532, 935, 1412
RsaI	GTAC	3 cut(s)	541, 852, 1019
RsaNI	GTAC	3 cut(s)	540, 851, 1018
RseI	CAYNNNNRTG	4 cut(s)	1025, 1455, 1485, 1767
SacI	GAGCTC	3 cut(s)	910, 1644, 1893
SacII	CCGCGG	1 cut(s)	2094
SapI	GCTCTTC	2 cut(s)	1632, 1778
SaqAI	TTAA	5 cut(s)	132, 522, 629, 1065, 1320
Sau96I	GGNCC	3 cut(s)	4, 640, 794
SchI	GAGTC	3 cut(s)	1024, 1121, 1436
ScrFI	CCNGG	3 cut(s)	801, 1140, 1272
SduI	GDGCHC	7 cut(s)	487, 592, 910, 1495, 1644, 1796, 1893
SfaNI	GCATC	3 cut(s)	46, 970, 1584
SfcI	CTRYAG	3 cut(s)	984, 2070, 2112
Sfr303I	CCGCGG	1 cut(s)	2094
SgrBI	CCGCGG	1 cut(s)	2094
SinI	GGWCC	1 cut(s)	640
SmiMI	CAYNNNNRTG	4 cut(s)	1025, 1455, 1485, 1767
SmlI	CTYRAG	2 cut(s)	138, 1319
SmoI	CTYRAG	2 cut(s)	138, 1319
Sse9I	AATT	9 cut(s)	126, 169, 552, 717, 831, 878, 1080, 1102, 1765
SspMI	CTAG	8 cut(s)	75, 504, 560, 648, 660, 710, 1260, 1803
SstI	GAGCTC	3 cut(s)	910, 1644, 1893
StyD4I	CCNGG	3 cut(s)	799, 1138, 1270
StyI	CCWWGG	1 cut(s)	1329
TaaI	ACNGT	5 cut(s)	231, 544, 954, 1146, 2022
TaiI	ACGT	5 cut(s)	190, 444, 1311, 1968, 2018
TaqI	TCGA	3 cut(s)	40, 733, 910
TasI	AATT	9 cut(s)	126, 169, 552, 717, 831, 878, 1080, 1102, 1765
TatI	WGTACW	1 cut(s)	1017
TauI	GCSGC	8 cut(s)	1097, 1606, 2038, 2090, 2093, 2096, 2099, 2102
TfiI	GAWTC	2 cut(s)	656, 1036
Tru1I	TTAA	5 cut(s)	132, 522, 629, 1065, 1320
Tru9I	TTAA	5 cut(s)	132, 522, 629, 1065, 1320
TscAI	CASTG	6 cut(s)	811, 874, 880, 1122, 1353, 1489
TseFI	GTSAC	5 cut(s)	948, 1113, 1428, 1756, 2016
Tsp45I	GTSAC	5 cut(s)	948, 1113, 1428, 1756, 2016
TspGWI	ACGGA	1 cut(s)	1600
TspRI	CASTG	6 cut(s)	811, 874, 880, 1122, 1353, 1489
Vha464I	CTTAAG	1 cut(s)	1319
VneI	GTGCAC	1 cut(s)	483
VpaK11BI	GGWCC	1 cut(s)	640
XapI	RAATTY	1 cut(s)	878
XbaI	TCTAGA	1 cut(s)	74
XceI	RCATGY	1 cut(s)	1216
XmiI	GTMKAC	1 cut(s)	452
XmnI	GAANNNNTTC	1 cut(s)	720
XspI	CTAG	8 cut(s)	75, 504, 560, 648, 660, 710, 1260, 1803

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.