Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003616

Physical Location & Seq

Genomic Coordinates Forward (+)

3916201 .. 3920556

Gene Structure 4356 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1058500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGAATGCTGTGCTCGAGGTTTCAAGGCATCGATCAGAGTTGGGACAACTGACAAAAGTGGTTACCAGAACTTATCATCCTACCAACAGGTTAGGAGGAGGTGCCGGAGGGAATAAGTTTTTAAGTACTCAAGAAAGATTCAAGTCCAGTTATGTTGCCAATTTCGGCCGTAGAGTACGCGATACCGATGAAGCTAGTGAAATTTCCTATCTAAAAGAACTTTACCATCGGAGTGATTATGAAGCAGTGATAAGAGCATTTGAAAGTCAACCTTCATTACATTCTAACCCTTCAGCACTTTCTGAATATGTGAAAGCATTAGTGAGAGTTGGCAGGCTGGATGACAGTGGGTTACTCAAGACATTGCAGAGAGGTGTTGCTAACTCTGCAAGGGAAGAAGAAAGCATTGCCAGCTTCTCAGTTCTCAAGAATTTTGGAAAAACAACGAAAGAAGGTATTCTTGGAACTGCAAGTGCTCCTATTCATATGGTGGCTACAGAAGGAGGTCAGTTTAAAGAGCAGCTGTGGCGTACAATTCGCTCTATCGCACTGGCCTTTCTTTTGATATCTGGTATAGGAGCACTCATTGAGGATAAAGGAATAAGCAAAGGACTCGGACTAAATGAGGAGGTTCAACCTATTGTGGAATCAAGCACAAAATTTAGTGATGTAAAGGGTGTTGATGAGGCTAAATCTGAGTTAGAGGAAATTGTTTACTATCTTCGAGATCCTAAGCGTTTCACTCGTCTAGGTGGCAAGCTCCCAAAAGGTGTTTTGCTTGTTGGTCCACCTGGCACTGGGAAAACCATGTTAGCAAGAGCCATTGCTGGAGAAGCTGGGGTACCTTTCTTTTCATGCAGCGGCAGTGAGTTTGAAGAGATGTTTGTTGGTGTAGGTGCTCGAAGGGTGAGGGATCTTTTCTCGGCTGCAAAGAAGCAATCACCTTGTATAATATTCATTGATGAGATAGACGTAATAGGAGGAAGCCGAAACCCAATGGATCAGAAGTACATGAAGATGACCCTCAATCAGTTACTTGTCGAACTTGATGGTTTTAAACAAAATGAAGGGATTATTGTCATTGCTGCTACTAATTTCCCAGAGTCTCTGGACAAGGCATTGGTGAGACCTGGGCGGTTTGATCGGCACATTGTTGTCCCTAATCCTGATGTTGAAGGGCGAAGGCAGATTATGGAAGCCCACATGTCGAAGGTTCTGAAGGCCAATGACGTTGATTTGAGTATTATTGCCAGAGGAACTCCGGGGTTCTCCGGAGCTGATCTTGCGAACTTGATCAACATTGCTGCTCTGAAAGCTGCCATGGACGGTGCTAAAGCAGTGAACATGGCTGATCTTGAATATGCAAAAGACAAAATTATGATGGGAAGCGAACGCAAGTCAGCTGTTATATCTGATGAGTCAAGAAAGGTAACAGCTTTTCATGAGGGTGGCCATGCACTTGTAGCAATCCACACTGATGGTGCTCATCCAGTTCACAAGGCAACAATAGTTCCTAGGGGTATGGCACTTGGCATGGTTATGCAGCTACCTGACAAGGACCAGACAAGTGTCTCCCGGAAACAGATGCTTGCACGGCTCGACGTTTGTATGGGGGGGCGTGTTGCAGAAGAGCTCATTTTTGGAGAAAGTGAAGTTACTTCGGGTGCATCAAATGATCTCCAGCAAGCAACTTCGCTTGCTAGAGCAATGGTTACAAAGTACGGCATGAGCAAACAAGTTGGGCTCGTCAGTCACAATTACGACGACAATGGTAAGAGCATGAGCACAGAAACTAGGCTGCTCATCGAAGAAGAAGTGAAGTACTTATTGGAAAGGGCATACGACAATGCAAAAACCATCCTCACCGAACACAGCAAGGAACATCACGCCCTTGCTAATGCTTTACTGGAGGAGGAAACACTCACCGGAACTCAGATCAAATCCTTGCTCGCACAAGTAAGCTCGCAGCAACAGCAGCAACCGCACCAGCAAGTAGTTGCAGCGAAGAGCAACTCGCAGTCTAAGCCAGTGCCACCCTCCACCCCCAACGCAGCTGCATCTGCTGCTGCGGCTGCATCCGCCGCTGCCGCTGCCGCAACTGCTGCGGCCAAGTCAACAGGTATTGCTCCAGTTGGATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009536 GO:0009888 GO:0009941 GO:0010073 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048507 GO:0048856 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04139 map04139

Pfam Domains

Protein Families

AAA Peptidase_M41

Protein Analysis

714

Amino Acids

76.83

Weight (kDa)

8.14

Isoelectric Point (pI)

40.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_5	PF07728	257 - 375	2.4e-07	AAA domain (dynein-related subfamily)
AAA	PF00004	258 - 387	2.6e-45	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	410 - 453	2.7e-12	AAA+ lid domain
Peptidase_M41	PF01434	469 - 649	7.6e-68	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003616)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26140
fragaria_vesca	FvH4_1g07720 FvH4_2g31730 FvH4_2g31730
malus_domestica	MD08G1063300.v1.1 MD15G1058100.v1.1 MD15G1058500.v1.1
prunus_persica	Prupe.1G410800_v2.0.a1
pyrus_communis	pycom08g05200 pycom15g05470 pycom15g05500
rosa_chinensis	RchiOBHm_Chr2g0093601 RchiOBHm_Chr6g0311091
rosa_laevigata	RLG00000010436 RLG00000016420
rosa_multiflora	Rmu_co8327861.1_g000001 Rmu_co8491229.1_g000001 Rmu_sc0002126.1_g000022 Rmu_sc0008967.1_g000002 Rmu_sc0012235.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147960 Rroxscaffold_7G00158030
rosa_rugosa	Rorug02G0037800 Rorug02G0037800 Rorug06G0385300 Rorug06G0385400
rosa_samantha	Rh2AG084300 Rh2BG084900 Rh2CG087200 Rh2DG082900 Rh6AG499400 Rh6BG509900 Rh6CG515700 Rh6DG502300
rosa_wichuraiana	Rw6G043500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	841
AccB1I	GGYRCC	2 cut(s)	101, 841
AccII	CGCG	1 cut(s)	180
AccIII	TCCGGA	1 cut(s)	1271
AciI	CCGC	9 cut(s)	861, 1135, 1982, 2069, 2079, 2082, 2088, 2094, 2105
AclWI	GGATC	3 cut(s)	722, 921, 1008
AcoI	YGGCCR	3 cut(s)	166, 1450, 2106
AcsI	RAATTY	3 cut(s)	201, 430, 659
AcuI	CTGAAG	2 cut(s)	276, 1238
AfaI	GTAC	7 cut(s)	127, 177, 532, 843, 1010, 1721, 1823
AfiI	CCNNNNNNNGG	1 cut(s)	797
AflIII	ACRYGT	1 cut(s)	1203
AgsI	TTSAA	7 cut(s)	24, 142, 263, 635, 875, 1175, 1358
AjnI	CCWGG	2 cut(s)	790, 1129
AjuI	GAANNNNNNNTTGG	4 cut(s)	444, 476, 1811, 1843
AloI	GAACNNNNNNTCC	2 cut(s)	61, 93
Alw21I	GWGCWC	7 cut(s)	15, 478, 583, 901, 1486, 1635, 1787
Alw26I	GTCTC	3 cut(s)	1110, 1120, 1576
AlwI	GGATC	3 cut(s)	722, 921, 1008
Ama87I	CYCGRG	1 cut(s)	14
Aor13HI	TCCGGA	1 cut(s)	1271
AoxI	GGCC	5 cut(s)	166, 552, 1221, 1450, 2106
ApoI	RAATTY	3 cut(s)	201, 430, 659
Asp700I	GAANNNNTTC	1 cut(s)	456
Asp718I	GGTACC	1 cut(s)	841
AspA2I	CCTAGG	1 cut(s)	1514
AspS9I	GGNCC	2 cut(s)	785, 1558
AsuC2I	CCSGG	2 cut(s)	1263, 1576
AsuHPI	GGTGA	5 cut(s)	919, 933, 1135, 1855, 1915
AvaI	CYCGRG	1 cut(s)	14
AvaII	GGWCC	2 cut(s)	785, 1558
AvrII	CCTAGG	1 cut(s)	1514
BalI	TGGCCA	1 cut(s)	1452
BanI	GGYRCC	2 cut(s)	101, 841
BanII	GRGCYC	2 cut(s)	1635, 1746
BarI	GAAGNNNNNNTAC	2 cut(s)	825, 857
Bbv12I	GWGCWC	7 cut(s)	15, 478, 583, 901, 1486, 1635, 1787
BccI	CCATC	5 cut(s)	234, 1043, 1375, 1472, 1865
BceAI	ACGGC	3 cut(s)	153, 1610, 1738
BcgI	CGANNNNNNTGC	2 cut(s)	595, 629
BciT130I	CCWGG	2 cut(s)	792, 1131
BclI	TGATCA	1 cut(s)	1293
BcnI	CCSGG	2 cut(s)	1263, 1576
BcoDI	GTCTC	3 cut(s)	1110, 1120, 1576
BfaI	CTAG	5 cut(s)	195, 749, 1515, 1701, 1794
BfmI	CTRYAG	1 cut(s)	495
BlnI	CCTAGG	1 cut(s)	1514
BmcAI	AGTACT	2 cut(s)	127, 1823
Bme1390I	CCNGG	4 cut(s)	792, 1131, 1263, 1576
Bme18I	GGWCC	2 cut(s)	785, 1558
BmeT110I	CYCGRG	1 cut(s)	14
BmgT120I	GGNCC	2 cut(s)	785, 1558
BmiI	GGNNCC	2 cut(s)	103, 843
BmrFI	CCNGG	4 cut(s)	792, 1131, 1263, 1576
BmrI	ACTGGG	1 cut(s)	807
BmsI	GCATC	5 cut(s)	37, 1575, 1676, 2066, 2084
BmuI	ACTGGG	1 cut(s)	807
BoxI	GACNNNNGTC	1 cut(s)	1568
BpmI	CTGGAG	4 cut(s)	849, 1664, 1928, 2112
Bpu10I	CCTNAGC	1 cut(s)	732
BpuEI	CTTGAG	3 cut(s)	114, 341, 410
BpuMI	CCSGG	2 cut(s)	1263, 1576
Bsa29I	ATCGAT	1 cut(s)	31
BsaI	GGTCTC	1 cut(s)	1120
BsaJI	CCNNGG	4 cut(s)	1130, 1262, 1320, 1514
BsaWI	WCCGGW	2 cut(s)	1271, 1925
Bsc4I	CCNNNNNNNGG	1 cut(s)	797
Bse1I	ACTGG	7 cut(s)	147, 555, 802, 1490, 1911, 2027, 2129
Bse3DI	GCAATG	6 cut(s)	362, 405, 822, 1080, 1299, 1713
BseAI	TCCGGA	1 cut(s)	1271
BseBI	CCWGG	2 cut(s)	792, 1131
BseCI	ATCGAT	1 cut(s)	31
BseDI	CCNNGG	4 cut(s)	1130, 1262, 1320, 1514
BseGI	GGATG	5 cut(s)	76, 346, 1486, 1857, 2075
BseLI	CCNNNNNNNGG	1 cut(s)	797
BseMI	GCAATG	6 cut(s)	362, 405, 822, 1080, 1299, 1713
BseMII	CTCAG	3 cut(s)	432, 687, 1946
BseNI	ACTGG	7 cut(s)	147, 555, 802, 1490, 1911, 2027, 2129
BseRI	GAGGAG	3 cut(s)	111, 641, 1925
BseX3I	CGGCCG	1 cut(s)	166
BseYI	CCCAGC	1 cut(s)	836
Bsh1236I	CGCG	1 cut(s)	180
Bsh1285I	CGRYCG	1 cut(s)	169
BshFI	GGCC	5 cut(s)	168, 554, 1223, 1452, 2108
BshNI	GGYRCC	2 cut(s)	101, 841
BshVI	ATCGAT	1 cut(s)	31
BsiEI	CGRYCG	1 cut(s)	169
BsiHKAI	GWGCWC	7 cut(s)	15, 478, 583, 901, 1486, 1635, 1787
BsiHKCI	CYCGRG	1 cut(s)	14
BsiSI	CCGG	5 cut(s)	105, 1262, 1272, 1576, 1926
BslFI	GGGAC	2 cut(s)	57, 1142
BslI	CCNNNNNNNGG	1 cut(s)	797
BsmAI	GTCTC	3 cut(s)	1110, 1120, 1576
BsmFI	GGGAC	2 cut(s)	57, 1142
BsmI	GAATGC	1 cut(s)	10
BsnI	GGCC	5 cut(s)	168, 554, 1223, 1452, 2108
Bso31I	GGTCTC	1 cut(s)	1120
BsoBI	CYCGRG	1 cut(s)	14
Bsp1286I	GDGCHC	8 cut(s)	15, 478, 583, 901, 1486, 1635, 1746, 1787
Bsp13I	TCCGGA	1 cut(s)	1271
Bsp19I	CCATGG	1 cut(s)	1320
BspACI	CCGC	9 cut(s)	861, 1135, 1982, 2069, 2079, 2082, 2088, 2094, 2105
BspANI	GGCC	5 cut(s)	168, 554, 1223, 1452, 2108
BspCNI	CTCAG	3 cut(s)	431, 688, 1945
BspDI	ATCGAT	1 cut(s)	31
BspEI	TCCGGA	1 cut(s)	1271
BspFNI	CGCG	1 cut(s)	180
BspHI	TCATGA	1 cut(s)	1441
BspLI	GGNNCC	2 cut(s)	103, 843
BspPI	GGATC	3 cut(s)	722, 921, 1008
BspQI	GCTCTTC	2 cut(s)	1623, 2000
BspT107I	GGYRCC	2 cut(s)	101, 841
BspTNI	GGTCTC	1 cut(s)	1120
BsrDI	GCAATG	6 cut(s)	362, 405, 822, 1080, 1299, 1713
BsrI	ACTGG	7 cut(s)	147, 555, 802, 1490, 1911, 2027, 2129
BssECI	CCNNGG	4 cut(s)	1130, 1262, 1320, 1514
BssT1I	CCWWGG	2 cut(s)	1320, 1514
Bst2UI	CCWGG	2 cut(s)	792, 1131
Bst4CI	ACNGT	2 cut(s)	347, 1328
Bst6I	CTCTTC	3 cut(s)	870, 1623, 2000
BstAPI	GCANNNNNTGC	2 cut(s)	2063, 2102
BstC8I	GCNNGC	8 cut(s)	335, 412, 758, 1590, 1686, 1698, 1950, 1964
BstDEI	CTNAG	6 cut(s)	418, 696, 732, 1932, 2022, 2139
BstDSI	CCRYGG	1 cut(s)	1320
BstEII	GGTNACC	1 cut(s)	61
BstF5I	GGATG	5 cut(s)	76, 346, 1486, 1857, 2075
BstFNI	CGCG	1 cut(s)	180
BstMAI	GTCTC	3 cut(s)	1110, 1120, 1576
BstMCI	CGRYCG	1 cut(s)	169
BstNI	CCWGG	2 cut(s)	792, 1131
BstNSI	RCATGY	1 cut(s)	1207
BstPAI	GACNNNNGTC	1 cut(s)	1568
BstPI	GGTNACC	1 cut(s)	61
BstSCI	CCNGG	4 cut(s)	790, 1129, 1261, 1574
BstSFI	CTRYAG	1 cut(s)	495
BstUI	CGCG	1 cut(s)	180
BstX2I	RGATCY	3 cut(s)	727, 913, 2135
BstXI	CCANNNNNNTGG	1 cut(s)	1478
BstYI	RGATCY	3 cut(s)	727, 913, 2135
BstZI	CGGCCG	1 cut(s)	166
Bsu15I	ATCGAT	1 cut(s)	31
BsuRI	GGCC	5 cut(s)	168, 554, 1223, 1452, 2108
BsuTUI	ATCGAT	1 cut(s)	31
BtgI	CCRYGG	1 cut(s)	1320
BtsCI	GGATG	5 cut(s)	76, 346, 1486, 1857, 2075
BtsI	GCAGTG	3 cut(s)	252, 871, 1344
BtsIMutI	CAGTG	8 cut(s)	252, 352, 548, 795, 871, 1344, 1473, 2034
Cac8I	GCNNGC	8 cut(s)	335, 412, 758, 1590, 1686, 1698, 1950, 1964
CciI	TCATGA	1 cut(s)	1441
Cfr13I	GGNCC	2 cut(s)	785, 1558
ClaI	ATCGAT	1 cut(s)	31
Csp6I	GTAC	7 cut(s)	126, 176, 531, 842, 1009, 1720, 1822
CviQI	GTAC	7 cut(s)	126, 176, 531, 842, 1009, 1720, 1822
DdeI	CTNAG	6 cut(s)	418, 696, 732, 1932, 2022, 2139
DraI	TTTAAA	2 cut(s)	514, 1057
EaeI	YGGCCR	3 cut(s)	166, 1450, 2106
EagI	CGGCCG	1 cut(s)	166
Eam1104I	CTCTTC	3 cut(s)	870, 1623, 2000
EarI	CTCTTC	3 cut(s)	870, 1623, 2000
EciI	GGCGGA	1 cut(s)	2068
Ecl136II	GAGCTC	1 cut(s)	1633
EclXI	CGGCCG	1 cut(s)	166
Eco130I	CCWWGG	2 cut(s)	1320, 1514
Eco24I	GRGCYC	2 cut(s)	1635, 1746
Eco31I	GGTCTC	1 cut(s)	1120
Eco32I	GATATC	1 cut(s)	567
Eco47I	GGWCC	2 cut(s)	785, 1558
Eco52I	CGGCCG	1 cut(s)	166
Eco53kI	GAGCTC	1 cut(s)	1633
Eco57I	CTGAAG	2 cut(s)	276, 1238
Eco88I	CYCGRG	1 cut(s)	14
Eco91I	GGTNACC	1 cut(s)	61
EcoICRI	GAGCTC	1 cut(s)	1633
EcoO65I	GGTNACC	1 cut(s)	61
EcoRII	CCWGG	2 cut(s)	790, 1129
EcoRV	GATATC	1 cut(s)	567
EcoT14I	CCWWGG	2 cut(s)	1320, 1514
EcoT38I	GRGCYC	2 cut(s)	1635, 1746
ErhI	CCWWGG	2 cut(s)	1320, 1514
FalI	AAGNNNNNCTT	4 cut(s)	256, 288, 444, 476
FaqI	GGGAC	2 cut(s)	57, 1142
FauNDI	CATATG	1 cut(s)	486
FbaI	TGATCA	1 cut(s)	1293
FokI	GGATG	5 cut(s)	63, 353, 1473, 1844, 2062
FriOI	GRGCYC	2 cut(s)	1635, 1746
FspBI	CTAG	5 cut(s)	195, 749, 1515, 1701, 1794
GsaI	CCCAGC	1 cut(s)	840
GsuI	CTGGAG	4 cut(s)	849, 1664, 1928, 2112
HaeIII	GGCC	5 cut(s)	168, 554, 1223, 1452, 2108
HapII	CCGG	5 cut(s)	105, 1262, 1272, 1576, 1926
HincII	GTYRAC	2 cut(s)	269, 2115
HindII	GTYRAC	2 cut(s)	269, 2115
HinfI	GANTC	5 cut(s)	138, 612, 647, 1103, 1418
HpaII	CCGG	5 cut(s)	105, 1262, 1272, 1576, 1926
HphI	GGTGA	5 cut(s)	919, 933, 1135, 1855, 1915
Hpy166II	GTNNAC	6 cut(s)	269, 715, 788, 1342, 1495, 2115
Hpy8I	GTNNAC	6 cut(s)	269, 715, 788, 1342, 1495, 2115
Hpy99I	CGWCG	2 cut(s)	1604, 1766
HpyCH4III	ACNGT	2 cut(s)	347, 1328
HpyCH4IV	ACGT	3 cut(s)	972, 1230, 1602
HpyF3I	CTNAG	6 cut(s)	418, 696, 732, 1932, 2022, 2139
HpySE526I	ACGT	3 cut(s)	972, 1230, 1602
Kpn2I	TCCGGA	1 cut(s)	1271
KpnI	GGTACC	1 cut(s)	845
Ksp22I	TGATCA	1 cut(s)	1293
LguI	GCTCTTC	2 cut(s)	1623, 2000
LmnI	GCTCC	5 cut(s)	481, 578, 765, 1274, 2131
LweI	GCATC	5 cut(s)	37, 1575, 1676, 2066, 2084
MaeI	CTAG	5 cut(s)	195, 749, 1515, 1701, 1794
MaeII	ACGT	3 cut(s)	972, 1230, 1602
MaeIII	GTNAC	7 cut(s)	61, 351, 1032, 1429, 1654, 1711, 1751
MboII	GAAGA	9 cut(s)	407, 410, 713, 887, 1027, 1640, 1820, 1823, 2017
MflI	RGATCY	3 cut(s)	727, 913, 2135
MhlI	GDGCHC	8 cut(s)	15, 478, 583, 901, 1486, 1635, 1746, 1787
MlsI	TGGCCA	1 cut(s)	1452
MluCI	AATT	9 cut(s)	160, 201, 430, 534, 659, 708, 1093, 1374, 1756
MluNI	TGGCCA	1 cut(s)	1452
MlyI	GAGTC	3 cut(s)	606, 1112, 1427
MmeI	TCCRAC	1 cut(s)	2113
Mox20I	TGGCCA	1 cut(s)	1452
MroI	TCCGGA	1 cut(s)	1271
MroXI	GAANNNNTTC	1 cut(s)	456
MscI	TGGCCA	1 cut(s)	1452
MseI	TTAA	3 cut(s)	122, 513, 1056
MslI	CAYNNNNRTG	5 cut(s)	231, 1016, 1446, 1476, 1538
Msp20I	TGGCCA	1 cut(s)	1452
MspA1I	CMGCKG	6 cut(s)	523, 861, 1403, 2054, 2084, 2090
MspI	CCGG	5 cut(s)	105, 1262, 1272, 1576, 1926
MspR9I	CCNGG	4 cut(s)	792, 1131, 1263, 1576
Mva1269I	GAATGC	1 cut(s)	10
MvaI	CCWGG	2 cut(s)	792, 1131
MvnI	CGCG	1 cut(s)	180
NciI	CCSGG	2 cut(s)	1263, 1576
NcoI	CCATGG	1 cut(s)	1320
NdeI	CATATG	1 cut(s)	486
NlaIV	GGNNCC	2 cut(s)	103, 843
NmeAIII	GCCGAG	1 cut(s)	902
NmuCI	GTSAC	1 cut(s)	1751
NspI	RCATGY	1 cut(s)	1207
PaeR7I	CTCGAG	1 cut(s)	14
PagI	TCATGA	1 cut(s)	1441
PciI	ACATGT	1 cut(s)	1203
PciSI	GCTCTTC	2 cut(s)	1623, 2000
PctI	GAATGC	1 cut(s)	10
PdmI	GAANNNNTTC	1 cut(s)	456
PfeI	GAWTC	2 cut(s)	138, 647
PfoI	TCCNGGA	1 cut(s)	1574
PleI	GAGTC	3 cut(s)	606, 1111, 1426
PpsI	GAGTC	3 cut(s)	606, 1111, 1426
PscI	ACATGT	1 cut(s)	1203
PshAI	GACNNNNGTC	1 cut(s)	1568
Psp124BI	GAGCTC	1 cut(s)	1635
Psp6I	CCWGG	2 cut(s)	790, 1129
PspEI	GGTNACC	1 cut(s)	61
PspFI	CCCAGC	1 cut(s)	836
PspGI	CCWGG	2 cut(s)	790, 1129
PspN4I	GGNNCC	2 cut(s)	103, 843
PspPI	GGNCC	2 cut(s)	785, 1558
PspXI	VCTCGAGB	1 cut(s)	14
PsuI	RGATCY	3 cut(s)	727, 913, 2135
PvuII	CAGCTG	3 cut(s)	523, 1403, 2054
RsaI	GTAC	7 cut(s)	127, 177, 532, 843, 1010, 1721, 1823
RsaNI	GTAC	7 cut(s)	126, 176, 531, 842, 1009, 1720, 1822
RseI	CAYNNNNRTG	5 cut(s)	231, 1016, 1446, 1476, 1538
SacI	GAGCTC	1 cut(s)	1635
SapI	GCTCTTC	2 cut(s)	1623, 2000
SaqAI	TTAA	3 cut(s)	122, 513, 1056
Sau96I	GGNCC	2 cut(s)	785, 1558
ScaI	AGTACT	2 cut(s)	127, 1823
SchI	GAGTC	3 cut(s)	606, 1112, 1427
ScrFI	CCNGG	4 cut(s)	792, 1131, 1263, 1576
SduI	GDGCHC	8 cut(s)	15, 478, 583, 901, 1486, 1635, 1746, 1787
SfaNI	GCATC	5 cut(s)	37, 1575, 1676, 2066, 2084
SfcI	CTRYAG	1 cut(s)	495
Sfr274I	CTCGAG	1 cut(s)	14
SinI	GGWCC	2 cut(s)	785, 1558
SlaI	CTCGAG	1 cut(s)	14
SmiMI	CAYNNNNRTG	5 cut(s)	231, 1016, 1446, 1476, 1538
SmlI	CTYRAG	4 cut(s)	14, 129, 356, 425
SmoI	CTYRAG	4 cut(s)	14, 129, 356, 425
Sse9I	AATT	9 cut(s)	160, 201, 430, 534, 659, 708, 1093, 1374, 1756
SsiI	CCGC	9 cut(s)	861, 1135, 1982, 2069, 2079, 2082, 2088, 2094, 2105
SspI	AATATT	1 cut(s)	954
SspMI	CTAG	5 cut(s)	195, 749, 1515, 1701, 1794
SstI	GAGCTC	1 cut(s)	1635
StyD4I	CCNGG	4 cut(s)	790, 1129, 1261, 1574
StyI	CCWWGG	2 cut(s)	1320, 1514
TaaI	ACNGT	2 cut(s)	347, 1328
TaiI	ACGT	3 cut(s)	975, 1233, 1605
TaqI	TCGA	8 cut(s)	15, 31, 724, 901, 1041, 1208, 1599, 1806
TasI	AATT	9 cut(s)	160, 201, 430, 534, 659, 708, 1093, 1374, 1756
TatI	WGTACW	3 cut(s)	125, 1008, 1821
TauI	GCSGC	6 cut(s)	864, 2072, 2084, 2090, 2096, 2108
TfiI	GAWTC	2 cut(s)	138, 647
Tru1I	TTAA	3 cut(s)	122, 513, 1056
Tru9I	TTAA	3 cut(s)	122, 513, 1056
TscAI	CASTG	8 cut(s)	252, 352, 555, 802, 871, 1344, 1480, 2034
TseFI	GTSAC	1 cut(s)	1751
Tsp45I	GTSAC	1 cut(s)	1751
TspRI	CASTG	8 cut(s)	252, 352, 555, 802, 871, 1344, 1480, 2034
VpaK11BI	GGWCC	2 cut(s)	785, 1558
XapI	RAATTY	3 cut(s)	201, 430, 659
XceI	RCATGY	1 cut(s)	1207
XhoI	CTCGAG	1 cut(s)	14
XmaJI	CCTAGG	1 cut(s)	1514
XmnI	GAANNNNTTC	1 cut(s)	456
XspI	CTAG	5 cut(s)	195, 749, 1515, 1701, 1794
ZrmI	AGTACT	2 cut(s)	127, 1823

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.