Rh2CG087200

ATP-dependent zinc metalloprotease FTSH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003616
Physical Location & Seq
Reverse (-)
7193727 .. 7194179
453 bp
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UTR
Exon/CDS
Intron
Rh2CG087200.1

Sequence Viewer

Length: 453 bp
ATGTTTGTGGGTGTTGGAGCTGCGAGAGTGAGGAAGCTTTTTAATGCTGCAAAAGAGAAATCTCCCAGCATTATATTTATTGACGAAATAGATGCTGTTGGGGGACAACGAAGATCCGATGGCTATAGGGATACAGTGAACCAGTTGCTAGTCGAACTAGATGGCTTCAAACAAAATGATGGAGTAATTGTGATAGGTGCGACTAACTTTCTGGAATTATTGGATAAAGCTCTAGTGAGAGCTGGACGATTTGACCGTCAAATTTTTATCCATATGCCAGATGTTAAAGGACTGAGGGAGATATTGGAATCATACATGTCAAAAGTTGTTGCGGCAGATGGTGTAAACCTGGGTGCTAGAGGAACGAAGGAGTTTTCAACTGCTGATCTTGCAAACTTGGTCAACATTGCAGCCGTGAAAGCAGCAGTTGATGGTTCTAAACAAGTGAAGTGA

Protein Analysis

150

Amino Acids

16.25

Weight (kDa)

8.92

Isoelectric Point (pI)

28.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 2 - 91 1.6e-26 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 332
AclWI GGATC 1 cut(s) 108
AcsI RAATTY 1 cut(s) 261
AflIII ACRYGT 1 cut(s) 315
AgsI TTSAA 2 cut(s) 169, 378
AjnI CCWGG 1 cut(s) 348
AluBI AGCT 4 cut(s) 20, 37, 230, 242
AluI AGCT 4 cut(s) 20, 37, 230, 242
AlwI GGATC 1 cut(s) 108
ApeKI GCWGC 4 cut(s) 20, 47, 410, 422
ApoI RAATTY 1 cut(s) 261
BbvI GCAGC 4 cut(s) 7, 34, 422, 434
BccI CCATC 5 cut(s) 113, 155, 173, 332, 425
BceAI ACGGC 1 cut(s) 398
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BciT130I CCWGG 1 cut(s) 350
BciVI GTATCC 1 cut(s) 124
BfaI CTAG 4 cut(s) 149, 158, 233, 357
BfmI CTRYAG 1 cut(s) 124
BfuI GTATCC 1 cut(s) 124
BisI GCNGC 5 cut(s) 21, 48, 333, 411, 423
BlsI GCNGC 5 cut(s) 22, 49, 334, 412, 424
Bme1390I CCNGG 1 cut(s) 350
BmrFI CCNGG 1 cut(s) 350
BmsI GCATC 1 cut(s) 82
BsaJI CCNNGG 1 cut(s) 349
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bse1I ACTGG 1 cut(s) 142
Bse3DI GCAATG 1 cut(s) 405
BseBI CCWGG 1 cut(s) 350
BseDI CCNNGG 1 cut(s) 349
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 1 cut(s) 142
BseXI GCAGC 4 cut(s) 7, 34, 422, 434
BseYI CCCAGC 1 cut(s) 65
BslFI GGGAC 1 cut(s) 117
BsmFI GGGAC 1 cut(s) 117
Bsp143I GATC 2 cut(s) 113, 385
BspACI CCGC 1 cut(s) 332
BspCNI CTCAG 1 cut(s) 285
BspPI GGATC 1 cut(s) 108
BsrDI GCAATG 1 cut(s) 405
BsrI ACTGG 1 cut(s) 142
BssECI CCNNGG 1 cut(s) 349
BssMI GATC 2 cut(s) 113, 385
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 2 cut(s) 136, 257
BstDEI CTNAG 1 cut(s) 293
BstKTI GATC 2 cut(s) 116, 388
BstMBI GATC 2 cut(s) 113, 385
BstMWI GCNNNNNNNGC 2 cut(s) 389, 419
BstNI CCWGG 1 cut(s) 350
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 1 cut(s) 348
BstSFI CTRYAG 1 cut(s) 124
BstV1I GCAGC 4 cut(s) 7, 34, 422, 434
BstX2I RGATCY 1 cut(s) 113
BstYI RGATCY 1 cut(s) 113
BsuI GTATCC 1 cut(s) 124
BtsIMutI CAGTG 1 cut(s) 141
CviAII CATG 1 cut(s) 316
CviJI RGCY 7 cut(s) 20, 37, 123, 165, 230, 242, 413
CviKI_1 RGCY 7 cut(s) 20, 37, 123, 165, 230, 242, 413
DdeI CTNAG 1 cut(s) 293
DpnI GATC 2 cut(s) 115, 387
DpnII GATC 2 cut(s) 113, 385
EcoRII CCWGG 1 cut(s) 348
FaeI CATG 1 cut(s) 319
FaiI YATR 6 cut(s) 74, 126, 273, 275, 313, 317
FaqI GGGAC 1 cut(s) 117
FatI CATG 1 cut(s) 315
FauNDI CATATG 1 cut(s) 273
Fnu4HI GCNGC 5 cut(s) 21, 48, 333, 411, 423
Fsp4HI GCNGC 5 cut(s) 21, 48, 333, 411, 423
FspBI CTAG 4 cut(s) 149, 158, 233, 357
GluI GCNGC 5 cut(s) 21, 48, 333, 411, 423
GsaI CCCAGC 1 cut(s) 69
Hin1II CATG 1 cut(s) 319
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 1 cut(s) 308
Hpy166II GTNNAC 3 cut(s) 139, 346, 403
Hpy188I TCNGA 1 cut(s) 118
Hpy188III TCNNGA 1 cut(s) 212
Hpy8I GTNNAC 3 cut(s) 139, 346, 403
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 2 cut(s) 136, 257
HpyCH4V TGCA 3 cut(s) 50, 392, 410
HpyF10VI GCNNNNNNNGC 2 cut(s) 389, 419
HpyF3I CTNAG 1 cut(s) 293
Hsp92II CATG 1 cut(s) 319
Kzo9I GATC 2 cut(s) 113, 385
LmnI GCTCC 1 cut(s) 17
LpnPI CCDG 7 cut(s) 79, 155, 197, 228, 291, 335, 362
Lsp1109I GCAGC 4 cut(s) 7, 34, 422, 434
LweI GCATC 1 cut(s) 82
MaeI CTAG 4 cut(s) 149, 158, 233, 357
MalI GATC 2 cut(s) 115, 387
MboI GATC 2 cut(s) 113, 385
MboII GAAGA 1 cut(s) 123
MflI RGATCY 1 cut(s) 113
MluCI AATT 3 cut(s) 186, 215, 261
MnlI CCTC 3 cut(s) 24, 288, 353
MseI TTAA 2 cut(s) 42, 285
MspR9I CCNGG 1 cut(s) 350
MvaI CCWGG 1 cut(s) 350
MwoI GCNNNNNNNGC 2 cut(s) 389, 419
NdeI CATATG 1 cut(s) 273
NdeII GATC 2 cut(s) 113, 385
NlaIII CATG 1 cut(s) 319
NspI RCATGY 1 cut(s) 319
PciI ACATGT 1 cut(s) 315
PcsI WCGNNNNNNNCGW 1 cut(s) 253
PfeI GAWTC 1 cut(s) 308
PkrI GCNGC 5 cut(s) 22, 49, 334, 412, 424
PscI ACATGT 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 348
PspFI CCCAGC 1 cut(s) 65
PspGI CCWGG 1 cut(s) 348
PsuI RGATCY 1 cut(s) 113
SaqAI TTAA 2 cut(s) 42, 285
SatI GCNGC 5 cut(s) 21, 48, 333, 411, 423
Sau3AI GATC 2 cut(s) 113, 385
ScrFI CCNGG 1 cut(s) 350
SetI ASST 6 cut(s) 22, 39, 199, 232, 244, 351
SfaNI GCATC 1 cut(s) 82
SfcI CTRYAG 1 cut(s) 124
Sse9I AATT 3 cut(s) 186, 215, 261
SsiI CCGC 1 cut(s) 332
SspMI CTAG 4 cut(s) 149, 158, 233, 357
StyD4I CCNGG 1 cut(s) 348
TaaI ACNGT 2 cut(s) 136, 257
TaqI TCGA 1 cut(s) 153
TasI AATT 3 cut(s) 186, 215, 261
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 2 cut(s) 42, 285
Tru9I TTAA 2 cut(s) 42, 285
TscAI CASTG 1 cut(s) 141
TseI GCWGC 4 cut(s) 20, 47, 410, 422
TspRI CASTG 1 cut(s) 141
XapI RAATTY 1 cut(s) 261
XceI RCATGY 1 cut(s) 319
XspI CTAG 4 cut(s) 149, 158, 233, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.