MD08G1075700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003946
Physical Location & Seq
Forward (+)
6186036 .. 6188890
2855 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1075700.v1.1.491

Sequence Viewer

Length: 2226 bp
ATGAAACCCTCCGCTGCAGAAAAAGCCTTGCACTCTGCCAAACTTCACATAGAAGAAATTAGAACCAAAAAGTTCTCCATCGGAAAAACAAGGATAAACCCCTTAACTCTCGACCTCCACCACGCCGTCACTAGCCTCTCTGCTGAGCTCTACCAGAAAGACATCCACTTTCTAATGGAGCTCATACAGAATGCGGAAGATAATGAATACAAAAAAGGGATTGAACCGACGCTGGAATTTGTCCTGACAAAGAAGGACATAACTGGCAGTGGAGCTCCGGCTACTCTGCTTGTTTTTAACAATGAGGTCGGATTTTCCCGGAGCAACATTGATTCCATATGCAGTGTAGGCCGTTCTACTAAGAAGGGGAAAAGACATCTCGGGTTTATTGGAGAAAAAGGAATTGGGTTTAAAAGTGTGTTTCTTGTAAGTTCACAACCTCACATATTCAGCAATGGATATCGAATCAAATTCAGGGAAGAACCGAACAAAGATTGCAGTATTGGATACATTGTTCCTGAATGGGTTAGTGGGGAGCCTACTCTTTCGAGCATACTTGATGTATATGGTGCCACCAAGGTCGTGCCGGCAACTACAATTGTCCTTCCTTTAAAGCCTGAGAAGGTTGAAACAGTGAGGGCACAACTATCGGGTTTGCATCCTGAGCTTCTTCTTTTCTTATCCAAGGTAAACCGGGTATATGTACGTGGGTGTGATCCCAAAGCAGCCAATGGTGTCTCCAAAATTTCTATATGCACCGGGACTGATCTTATGAATGTCCGTGATAAGACTGCCAATGCACGTGTTGTTGAACTTTCGGTTAAAGAAAAAATGGGTGCCAGTGAGGAGAAGTGCAAGTATTACTTATGGAGACAGGCATTTCCAGTAAAACCTGCTAATAGAGTTAATACTAGGATGAATGTCGAGGAATGGGTGATCACTCTTGCTTTCCCATTTGGAAACAGACTGAGAAGAGATACTTCTTTTGTCGGTGTATTTGCTTTCCTGCCTACGGCCATGGTTACAAGCTTTCCCTTTCTCTCCCTGTTTCCTTCAGTGGGTCAGGCATTTCAGTTCATACCAACTCAAGCATCACCAATTCCGGTGTTTAATAAATTAACAGAGTCCATCAAAACTAGACTGCAAGGTCTCCAGATAGTACCCTGTGAAATATTTTCTAGCGAGAGGTTGTTTTTCCAGCCTAAAGAAGCAGTTAGAATCCTACATACATTCAGAGACCTCTTAGTTCGCATTAGAAGCAAAGGGGTTGTTGTAAGTGGTTTATCTTCTTTGATGAAAGTCTCACATTCATCTCTGAACTTTCAAGAAACCAGTGGAGTTATGGACTATCTAGGTGTAGTATATGCTTCATATAATTGGTATGCACAATGCATTCAATCCTGCAATATTGTGTCGCAAGTATCTGATGATGTCTACATTAAACTGCTTGGTTTTATTGCTAATACTTGTAAGGATACATCGTCCATACAATTGTTAGGTACCATGCCTCTTCTTAAATACATCAATCGTGAAGGGAATATGGAATTGCGTACAATTCCTAATACAACGCCCAAGATTGTATATAGTGTGGAGCTGGAGATTCACACATGGCTGAGTAAGTGCAATATGGAGTTTGGATGTCTCGGTAATTTTTATTTCTTGCCCAATAGCACTCGGGAAGCACTTTTAACTCACAAAAGAAGTTCATTTCTCCTCGAATGGCTTTCCAGTAATGCAGGTTTAAGTCCTTGTTCGGCAACAGAATTTGTTTCCTGGCTTTATCCTTATGTATCAAATAAGGAACCACTTCTTCCAGTCACCTTGGTCCATTTCCTTTATCATGCCCATATGAAAAACATCATTGATGATTCTCAATTGTATTCCTTTCTCACGGTGATCCCACTTATTGATGGGACTAACCAAGTCAGAATGCAAAGAACTGCAACTCTTGTCTCGGCATCAAGGAGCAAATGGGTGAAGTTGTTGGGACCTCTAAATCCATTCGTGGGACAATATTACATTGATATAGGAGATGTTTATGCTAAAAGTAGTATGCTTCTTGGAGAATTTGTGCCTCAGGATGAGCTTCTTCACTTTGTTGTTAAGTTCAGTAAAGCTGTGGACATTCCTGAGTTATGTCCTCCAGATGTGGTGTTACAGATAGCATCTCATGAACTATCAAGTGAGAAAGCATTTCTGTTGCTCGACTGGATCAGATTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

742

Amino Acids

83.2

Weight (kDa)

8.95

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 51 - 153 1.5e-09 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1146
Acc36I ACCTGC 2 cut(s) 901, 1727
Acc65I GGTACC 1 cut(s) 1499
AccB1I GGYRCC 3 cut(s) 569, 836, 1499
AccI GTMKAC 1 cut(s) 1434
AciI CCGC 2 cut(s) 12, 194
AclWI GGATC 3 cut(s) 710, 1891, 2219
AcoI YGGCCR 1 cut(s) 1014
AcsI RAATTY 5 cut(s) 236, 470, 744, 1763, 2066
AcuI CTGAAG 1 cut(s) 1038
AcvI CACGTG 1 cut(s) 803
AfaI GTAC 4 cut(s) 705, 1161, 1501, 1552
AfiI CCNNNNNNNGG 3 cut(s) 1012, 1058, 2006
AflIII ACRYGT 1 cut(s) 802
AgsI TTSAA 5 cut(s) 224, 629, 812, 1325, 1397
AjnI CCWGG 1 cut(s) 1772
AjuI GAANNNNNNNTTGG 2 cut(s) 387, 419
AloI GAACNNNNNNTCC 2 cut(s) 498, 530
AluBI AGCT 8 cut(s) 148, 181, 275, 667, 1029, 1594, 2086, 2117
AluI AGCT 8 cut(s) 148, 181, 275, 667, 1029, 1594, 2086, 2117
Alw21I GWGCWC 3 cut(s) 150, 183, 277
Alw26I GTCTC 7 cut(s) 742, 865, 1154, 1230, 1306, 1646, 1957
AlwI GGATC 3 cut(s) 710, 1891, 2219
Ama87I CYCGRG 2 cut(s) 380, 1674
AoxI GGCC 2 cut(s) 349, 1014
ApeKI GCWGC 2 cut(s) 14, 725
ApoI RAATTY 5 cut(s) 236, 470, 744, 1763, 2066
Asp700I GAANNNNTTC 1 cut(s) 1806
Asp718I GGTACC 1 cut(s) 1499
AspS9I GGNCC 2 cut(s) 1825, 1988
AsuC2I CCSGG 3 cut(s) 319, 695, 760
AsuHPI GGTGA 5 cut(s) 946, 1086, 1810, 1906, 1987
AvaI CYCGRG 2 cut(s) 380, 1674
AvaII GGWCC 2 cut(s) 1825, 1988
AxyI CCTNAGG 1 cut(s) 2076
BaeGI GKGCMC 1 cut(s) 643
BanI GGYRCC 3 cut(s) 569, 836, 1499
BanII GRGCYC 3 cut(s) 150, 183, 277
BbrPI CACGTG 1 cut(s) 803
Bbv12I GWGCWC 3 cut(s) 150, 183, 277
BbvI GCAGC 1 cut(s) 737
BccI CCATC 3 cut(s) 86, 1136, 1904
BceAI ACGGC 3 cut(s) 110, 336, 1029
BcgI CGANNNNNNTGC 2 cut(s) 630, 664
BciT130I CCWGG 1 cut(s) 1774
BciVI GTATCC 2 cut(s) 500, 1468
BclI TGATCA 1 cut(s) 936
BcnI CCSGG 3 cut(s) 319, 695, 760
BcoDI GTCTC 7 cut(s) 742, 865, 1154, 1230, 1306, 1646, 1957
BfaI CTAG 5 cut(s) 132, 912, 1137, 1179, 1352
BfmI CTRYAG 1 cut(s) 15
BfuAI ACCTGC 2 cut(s) 901, 1727
BfuI GTATCC 2 cut(s) 500, 1468
BisI GCNGC 2 cut(s) 15, 726
BlpI GCTNAGC 1 cut(s) 144
BlsI GCNGC 2 cut(s) 16, 727
Bme1390I CCNGG 4 cut(s) 319, 695, 760, 1774
Bme18I GGWCC 2 cut(s) 1825, 1988
BmeT110I CYCGRG 2 cut(s) 380, 1674
BmgT120I GGNCC 2 cut(s) 1825, 1988
BmiI GGNNCC 6 cut(s) 537, 571, 838, 1501, 1803, 1989
BmrFI CCNGG 4 cut(s) 319, 695, 760, 1774
BmsI GCATC 4 cut(s) 667, 1100, 1967, 2174
BpmI CTGGAG 3 cut(s) 1136, 1616, 2127
Bpu10I CCTNAGC 1 cut(s) 663
Bpu1102I GCTNAGC 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 1071
BpuMI CCSGG 3 cut(s) 319, 695, 760
BsaAI YACGTR 2 cut(s) 707, 803
BsaI GGTCTC 2 cut(s) 1154, 1230
BsaJI CCNNGG 4 cut(s) 576, 684, 1017, 1821
BsaWI WCCGGW 1 cut(s) 1102
BsaXI ACNNNNNCTCC 2 cut(s) 2055, 2085
Bsc4I CCNNNNNNNGG 3 cut(s) 1012, 1058, 2006
Bse118I RCCGGY 1 cut(s) 586
Bse1I ACTGG 7 cut(s) 268, 840, 884, 1332, 1728, 1814, 2213
Bse21I CCTNAGG 1 cut(s) 2076
Bse3DI GCAATG 1 cut(s) 460
BseBI CCWGG 1 cut(s) 1774
BseDI CCNNGG 4 cut(s) 576, 684, 1017, 1821
BseGI GGATG 5 cut(s) 162, 658, 921, 1643, 2086
BseLI CCNNNNNNNGG 3 cut(s) 1012, 1058, 2006
BseMI GCAATG 1 cut(s) 460
BseMII CTCAG 7 cut(s) 135, 609, 654, 959, 1604, 2090, 2121
BseNI ACTGG 7 cut(s) 268, 840, 884, 1332, 1728, 1814, 2213
BseRI GAGGAG 2 cut(s) 860, 1703
BseSI GKGCMC 1 cut(s) 643
BseXI GCAGC 1 cut(s) 737
BshFI GGCC 2 cut(s) 351, 1016
BshNI GGYRCC 3 cut(s) 569, 836, 1499
BsiHKAI GWGCWC 3 cut(s) 150, 183, 277
BsiHKCI CYCGRG 2 cut(s) 380, 1674
BsiSI CCGG 6 cut(s) 278, 319, 587, 694, 759, 1103
BslFI GGGAC 4 cut(s) 775, 1927, 2001, 2022
BslI CCNNNNNNNGG 3 cut(s) 1012, 1058, 2006
BsmAI GTCTC 7 cut(s) 742, 865, 1154, 1230, 1306, 1646, 1957
BsmFI GGGAC 4 cut(s) 775, 1927, 2001, 2022
BsmI GAATGC 3 cut(s) 196, 1392, 1935
BsnI GGCC 2 cut(s) 351, 1016
Bso31I GGTCTC 2 cut(s) 1154, 1230
BsoBI CYCGRG 2 cut(s) 380, 1674
Bsp1286I GDGCHC 4 cut(s) 150, 183, 277, 643
Bsp143I GATC 5 cut(s) 715, 766, 936, 1896, 2211
Bsp1720I GCTNAGC 1 cut(s) 144
Bsp19I CCATGG 1 cut(s) 1017
BspACI CCGC 2 cut(s) 12, 194
BspANI GGCC 2 cut(s) 351, 1016
BspCNI CTCAG 7 cut(s) 136, 610, 655, 960, 1605, 2089, 2122
BspHI TCATGA 1 cut(s) 2170
BspLI GGNNCC 6 cut(s) 537, 571, 838, 1501, 1803, 1989
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 2 cut(s) 901, 1727
BspPI GGATC 3 cut(s) 710, 1891, 2219
BspT107I GGYRCC 3 cut(s) 569, 836, 1499
BspTNI GGTCTC 2 cut(s) 1154, 1230
BsrDI GCAATG 1 cut(s) 460
BsrFI RCCGGY 1 cut(s) 586
BsrI ACTGG 7 cut(s) 268, 840, 884, 1332, 1728, 1814, 2213
BssAI RCCGGY 1 cut(s) 586
BssECI CCNNGG 4 cut(s) 576, 684, 1017, 1821
BssMI GATC 5 cut(s) 715, 766, 936, 1896, 2211
BssT1I CCWWGG 4 cut(s) 576, 684, 1017, 1821
Bst2UI CCWGG 1 cut(s) 1774
Bst4CI ACNGT 2 cut(s) 634, 1894
Bst6I CTCTTC 2 cut(s) 967, 1515
BstBAI YACGTR 2 cut(s) 707, 803
BstC8I GCNNGC 1 cut(s) 588
BstDEI CTNAG 9 cut(s) 144, 360, 618, 663, 968, 1243, 1613, 2076, 2130
BstDSI CCRYGG 1 cut(s) 1017
BstF5I GGATG 5 cut(s) 162, 658, 921, 1643, 2086
BstKTI GATC 5 cut(s) 718, 769, 939, 1899, 2214
BstMAI GTCTC 7 cut(s) 742, 865, 1154, 1230, 1306, 1646, 1957
BstMBI GATC 5 cut(s) 715, 766, 936, 1896, 2211
BstMWI GCNNNNNNNGC 4 cut(s) 23, 348, 664, 1257
BstNI CCWGG 1 cut(s) 1774
BstSCI CCNGG 4 cut(s) 317, 693, 758, 1772
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 1 cut(s) 643
BstV1I GCAGC 1 cut(s) 737
Bsu36I CCTNAGG 1 cut(s) 2076
BsuI GTATCC 2 cut(s) 500, 1468
BsuRI GGCC 2 cut(s) 351, 1016
BtgI CCRYGG 1 cut(s) 1017
BtsCI GGATG 5 cut(s) 162, 658, 921, 1643, 2086
BtsI GCAGTG 2 cut(s) 274, 349
BtsIMutI CAGTG 6 cut(s) 274, 349, 639, 847, 1062, 1339
BveI ACCTGC 2 cut(s) 901, 1727
Cac8I GCNNGC 1 cut(s) 588
CciI TCATGA 1 cut(s) 2170
Cfr10I RCCGGY 1 cut(s) 586
Cfr13I GGNCC 2 cut(s) 1825, 1988
CseI GACGC 1 cut(s) 238
Csp6I GTAC 4 cut(s) 704, 1160, 1500, 1551
CviAII CATG 5 cut(s) 1018, 1504, 1608, 1841, 2171
CviQI GTAC 4 cut(s) 704, 1160, 1500, 1551
DdeI CTNAG 9 cut(s) 144, 360, 618, 663, 968, 1243, 1613, 2076, 2130
DpnI GATC 5 cut(s) 717, 768, 938, 1898, 2213
DpnII GATC 5 cut(s) 715, 766, 936, 1896, 2211
DraI TTTAAA 2 cut(s) 412, 612
DrdI GACNNNNNNGTC 1 cut(s) 1146
DseDI GACNNNNNNGTC 1 cut(s) 1146
EaeI YGGCCR 1 cut(s) 1014
Eam1104I CTCTTC 2 cut(s) 967, 1515
EarI CTCTTC 2 cut(s) 967, 1515
Ecl136II GAGCTC 3 cut(s) 148, 181, 275
Eco130I CCWWGG 4 cut(s) 576, 684, 1017, 1821
Eco24I GRGCYC 3 cut(s) 150, 183, 277
Eco31I GGTCTC 2 cut(s) 1154, 1230
Eco32I GATATC 1 cut(s) 461
Eco47I GGWCC 2 cut(s) 1825, 1988
Eco53kI GAGCTC 3 cut(s) 148, 181, 275
Eco57I CTGAAG 1 cut(s) 1038
Eco72I CACGTG 1 cut(s) 803
Eco81I CCTNAGG 1 cut(s) 2076
Eco88I CYCGRG 2 cut(s) 380, 1674
EcoICRI GAGCTC 3 cut(s) 148, 181, 275
EcoO109I RGGNCCY 1 cut(s) 1988
EcoRII CCWGG 1 cut(s) 1772
EcoRV GATATC 1 cut(s) 461
EcoT14I CCWWGG 4 cut(s) 576, 684, 1017, 1821
EcoT22I ATGCAT 1 cut(s) 1394
EcoT38I GRGCYC 3 cut(s) 150, 183, 277
ErhI CCWWGG 4 cut(s) 576, 684, 1017, 1821
FaeI CATG 5 cut(s) 1021, 1507, 1611, 1844, 2174
FalI AAGNNNNNCTT 4 cut(s) 848, 880, 964, 996
FaqI GGGAC 4 cut(s) 775, 1927, 2001, 2022
FatI CATG 5 cut(s) 1017, 1503, 1607, 1840, 2170
FauNDI CATATG 2 cut(s) 338, 1848
FbaI TGATCA 1 cut(s) 936
FblI GTMKAC 1 cut(s) 1434
Fnu4HI GCNGC 2 cut(s) 15, 726
FokI GGATG 5 cut(s) 149, 645, 928, 1650, 2093
FriOI GRGCYC 3 cut(s) 150, 183, 277
Fsp4HI GCNGC 2 cut(s) 15, 726
FspBI CTAG 5 cut(s) 132, 912, 1137, 1179, 1352
GluI GCNGC 2 cut(s) 15, 726
GsuI CTGGAG 3 cut(s) 1136, 1616, 2127
HaeIII GGCC 2 cut(s) 351, 1016
HapII CCGG 6 cut(s) 278, 319, 587, 694, 759, 1103
HgaI GACGC 1 cut(s) 238
Hin1II CATG 5 cut(s) 1021, 1507, 1611, 1844, 2174
HindIII AAGCTT 1 cut(s) 1027
HinfI GANTC 6 cut(s) 332, 465, 1124, 1218, 1600, 1868
HpaII CCGG 6 cut(s) 278, 319, 587, 694, 759, 1103
HphI GGTGA 5 cut(s) 946, 1086, 1810, 1906, 1987
Hpy166II GTNNAC 4 cut(s) 434, 691, 1435, 2122
Hpy188I TCNGA 7 cut(s) 83, 311, 1235, 1317, 1426, 1928, 2216
Hpy8I GTNNAC 4 cut(s) 434, 691, 1435, 2122
Hpy99I CGWCG 1 cut(s) 232
HpyAV CCTTC 6 cut(s) 247, 358, 614, 616, 1062, 1526
HpyCH4III ACNGT 2 cut(s) 634, 1894
HpyCH4IV ACGT 2 cut(s) 706, 802
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 348, 664, 1257
HpyF3I CTNAG 9 cut(s) 144, 360, 618, 663, 968, 1243, 1613, 2076, 2130
HpySE526I ACGT 2 cut(s) 706, 802
Hsp92II CATG 5 cut(s) 1021, 1507, 1611, 1844, 2174
KpnI GGTACC 1 cut(s) 1503
KroI GCCGGC 1 cut(s) 586
KroNI GCCGGC 1 cut(s) 588
Ksp22I TGATCA 1 cut(s) 936
Kzo9I GATC 5 cut(s) 715, 766, 936, 1896, 2211
LmnI GCTCC 7 cut(s) 178, 272, 280, 321, 535, 1591, 1965
Lsp1109I GCAGC 1 cut(s) 737
LweI GCATC 4 cut(s) 667, 1100, 1967, 2174
MaeI CTAG 5 cut(s) 132, 912, 1137, 1179, 1352
MaeII ACGT 2 cut(s) 706, 802
MaeIII GTNAC 4 cut(s) 127, 1021, 1816, 2154
MalI GATC 5 cut(s) 717, 768, 938, 1898, 2213
MboI GATC 5 cut(s) 715, 766, 936, 1896, 2211
MboII GAAGA 9 cut(s) 65, 209, 491, 662, 984, 1278, 1502, 1802, 2081
MfeI CAATTG 3 cut(s) 597, 1490, 1874
MhlI GDGCHC 4 cut(s) 150, 183, 277, 643
MlyI GAGTC 1 cut(s) 1133
MmeI TCCRAC 1 cut(s) 289
Mph1103I ATGCAT 1 cut(s) 1394
MroNI GCCGGC 1 cut(s) 586
MroXI GAANNNNTTC 1 cut(s) 1806
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 6 cut(s) 278, 319, 587, 694, 759, 1103
MspR9I CCNGG 4 cut(s) 319, 695, 760, 1774
MunI CAATTG 3 cut(s) 597, 1490, 1874
Mva1269I GAATGC 3 cut(s) 196, 1392, 1935
MvaI CCWGG 1 cut(s) 1774
MwoI GCNNNNNNNGC 4 cut(s) 23, 348, 664, 1257
NaeI GCCGGC 1 cut(s) 588
NciI CCSGG 3 cut(s) 319, 695, 760
NcoI CCATGG 1 cut(s) 1017
NdeI CATATG 2 cut(s) 338, 1848
NdeII GATC 5 cut(s) 715, 766, 936, 1896, 2211
NgoMIV GCCGGC 1 cut(s) 586
NlaIII CATG 5 cut(s) 1021, 1507, 1611, 1844, 2174
NlaIV GGNNCC 6 cut(s) 537, 571, 838, 1501, 1803, 1989
NmeAIII GCCGAG 1 cut(s) 1934
NmuCI GTSAC 2 cut(s) 127, 1816
NsiI ATGCAT 1 cut(s) 1394
PagI TCATGA 1 cut(s) 2170
PctI GAATGC 3 cut(s) 196, 1392, 1935
PdiI GCCGGC 1 cut(s) 588
PdmI GAANNNNTTC 1 cut(s) 1806
PfeI GAWTC 5 cut(s) 332, 465, 1218, 1600, 1868
PfoI TCCNGGA 1 cut(s) 317
PkrI GCNGC 2 cut(s) 16, 727
PleI GAGTC 1 cut(s) 1132
PmaCI CACGTG 1 cut(s) 803
PmlI CACGTG 1 cut(s) 803
PpsI GAGTC 1 cut(s) 1132
Ppu21I YACGTR 2 cut(s) 707, 803
PpuMI RGGWCCY 1 cut(s) 1988
Psp124BI GAGCTC 3 cut(s) 150, 183, 277
Psp5II RGGWCCY 1 cut(s) 1988
Psp6I CCWGG 1 cut(s) 1772
PspCI CACGTG 1 cut(s) 803
PspGI CCWGG 1 cut(s) 1772
PspN4I GGNNCC 6 cut(s) 537, 571, 838, 1501, 1803, 1989
PspPI GGNCC 2 cut(s) 1825, 1988
PspPPI RGGWCCY 1 cut(s) 1988
PstI CTGCAG 1 cut(s) 19
RsaI GTAC 4 cut(s) 705, 1161, 1501, 1552
RsaNI GTAC 4 cut(s) 704, 1160, 1500, 1551
SacI GAGCTC 3 cut(s) 150, 183, 277
SatI GCNGC 2 cut(s) 15, 726
Sau3AI GATC 5 cut(s) 715, 766, 936, 1896, 2211
Sau96I GGNCC 2 cut(s) 1825, 1988
SchI GAGTC 1 cut(s) 1133
ScrFI CCNGG 4 cut(s) 319, 695, 760, 1774
SduI GDGCHC 4 cut(s) 150, 183, 277, 643
SfaNI GCATC 4 cut(s) 667, 1100, 1967, 2174
SfcI CTRYAG 1 cut(s) 15
SinI GGWCC 2 cut(s) 1825, 1988
SmlI CTYRAG 1 cut(s) 1086
SmoI CTYRAG 1 cut(s) 1086
SsiI CCGC 2 cut(s) 12, 194
SspI AATATT 3 cut(s) 1173, 1408, 2015
SspMI CTAG 5 cut(s) 132, 912, 1137, 1179, 1352
SstI GAGCTC 3 cut(s) 150, 183, 277
StyD4I CCNGG 4 cut(s) 317, 693, 758, 1772
StyI CCWWGG 4 cut(s) 576, 684, 1017, 1821
TaaI ACNGT 2 cut(s) 634, 1894
TaiI ACGT 2 cut(s) 709, 805
TaqI TCGA 6 cut(s) 111, 463, 548, 924, 1716, 2205
TfiI GAWTC 5 cut(s) 332, 465, 1218, 1600, 1868
TscAI CASTG 6 cut(s) 274, 349, 639, 847, 1062, 1339
TseFI GTSAC 2 cut(s) 127, 1816
TseI GCWGC 2 cut(s) 14, 725
Tsp45I GTSAC 2 cut(s) 127, 1816
TspGWI ACGGA 1 cut(s) 770
TspRI CASTG 6 cut(s) 274, 349, 639, 847, 1062, 1339
VpaK11BI GGWCC 2 cut(s) 1825, 1988
XapI RAATTY 5 cut(s) 236, 470, 744, 1763, 2066
XcmI CCANNNNNNNNNTGG 1 cut(s) 1339
XmiI GTMKAC 1 cut(s) 1434
XmnI GAANNNNTTC 1 cut(s) 1806
XspI CTAG 5 cut(s) 132, 912, 1137, 1179, 1352
Zsp2I ATGCAT 1 cut(s) 1394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.