Rw4G014340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003946
Physical Location & Seq
Reverse (-)
33275568 .. 33282620
7053 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G014340.1

Sequence Viewer

Length: 5259 bp
ATGGGAAATCCCAGCATTGAAGCCATGCGACACATAGAAGAGATCAGGACAAACAAGTTCTCATTAGGGAAGAAAGAACCAAACCCCTTAACTCTTGATCTTCACCATGCTGTGACCAGTCTATCTGCAGAGCTGTATCAGAAAGACATCCATTTCCTGATGGAGCTCATACAGAATGCTGAAGACAATGAGTACAAAGAAGGTGTAGAACCGACTCTGGAGTTTGTGCTGACCGAGAAAGACATAACTGGGACTGGAGCACCGGCTACTCTTTTGGTTTTCAATAATGAGGTTGGCTTTTCCAGGGAGAATATAGACTCCATTTGCAGCATTGGCCGTTCTACCAAGAAAGGGAAGAGACGTCAGGGCTTTATTGGAGAGAAAGGAATTGGATTCAAAAGTGTGTTTCTTGTGAGTTCACAACCTCACATATTTAGCAATGGATATCATGTCAAATTCAGGGAAGAACCAAACCAGGTCTGTGGTATTGGTTATATAGTTCCTGAATGGATTAGGGCTGCAGATTTGTCAGTATTAATGGTTCAGGCTGTGTATGGGCACGGCAAGGACTTGCCAACAACTACATTCTTACTTCCTCTAAAGCGAGACAAGGTGGAAGCAGTAAGAGCACAACTGTTGGAGCTGCACCCGGAGATTCTCTTGTTCTTATCCAAAATAAAGCGGCTATATGTACGTGGGTGTGATCCTCAAAAAGCTGATGATGTCTCAACATTATCTATATTTAGTGAGATTGAACATACAGAGTTAAGGAGTAAAACAGCCAATTCTCGTGTTGTGCAACTTTCTGTTAAGGAAAAAATGTGTGATACTGAGCAGTTGTGCAAGTATTACTTGTGGAGAGAGGAGTTTCCAGTGAAACCTGTGAATAGAGTTGAAATTAGGATGGATGTGGAGAAATGGGTGATTACTCTTGCTTTCCCATTTGGAAAAAGGCTGAGAAGAGGAACGTCTCCTGTTGGTATATTTGCTTTCCTGCCAACAACAATGGTCACAAACTTCCCCTTTGTAGTTCAAGCTGACTTCATCCTTGCTTCTTCGCGCGAATCCATAATTTTGGACAATTCATGGAACATGGGAATTCTTCAATGTGTCCCATCAGCCTTTGTGAATGCATTTCAACATTGCGTGGGACAATTGAACCTCTTTTCTTCTGTAAGTCAGCCATTTGAGTTTTTTCCTGCTCAAAACTCGCCAACGCCAGAGTTTAATACAGTACGAGGGTCCATTAGGACTAGGCTGCAAGGTCTAAACATAGTGCCCTGTAAAAGATTTTTTGGTGATAATCTTTCCTCCAAGCTGCCTCGACATGTAGTATGGATCAAGCCAAAATTTAGGGACCTGGTTGTTAAAATTAAAGCGGAAGGTGCTGTTTTTGGCAGTTCATCTTCATCTTCATTGAAGAGGGTCTTGGATTCATCTCTGGATGTTGAAAAATACAGGGACATTATGAACTTTCTAGGTGTGGCATATGCTGATGATGATTGGTACACAGCCTGCATCAACTCTTGTAATCTTTTGTTGTTATCAGATGAAGTCTACATTGAACTACTTGGTTTTTTGGCTGATAATGATGTAGAGTTTGACTCAGATAGCATTGAGACCATACCTCTTCTTAAGTATACTGATCGGAAAGGGAGTCCGGCCTTGTGTACAATTAAAACTACAGAAGAGGAACTCAAGATTCGATATGCTATGGGCCTGGAGCTTCACACATGGCTCAGCAAGTGCAATATGGATTTTGGCTGTCCTGATAATGTGTATTTCTTACCAAATAAGACACTGGAAGCTGTTTTGAATCACCAGAAAAGTCATCCCCCAAAGCGACGTTATAGAAAGAAAAATTACATGGAGAGTTCATCTGTCTGCAATTGGCTGAATAGAAGTGCTGGAGTGAAATCTCTTTCAGCATATGATTATGCTTTCTTACTTTTGAATCATGTATCGAAGAAGGAATCCAATGTTGCAGTCAGATTATCCAATTTCCTTTATCTTGCCCACAAGGAAGGCTTCCTCACTGAATATCAAATATCTGATCTTTGTGGTAGGATTCCAATTGTTGATGGGTCTGATAAAGTAAGAGTGCAAAGAACCGTAACTTTGGTCCCTTCATCAGGGAGCAAATGGGCCAAATTGTTTGGACTTCAAAATCCATTTGCTGAACAACATTATGTTGATATAGGGGATGTTTATGCTAAAAGCAGTTTGTTTCTGGGGAAATTCACCCCAGAGAAAGAGCTTCTGCATTTTGTTGGCGAGTATAGCAAAGCTACGGACTTACCCGACTTGTGTCCTCCTGATGTGGTGTTACAGATCGCATCTCATGAACTCTCGAGTGAGCAGGCCTTTTTACTCCTTGATTGGATCAGGTTACTGCGCACCAAGAATTCATCCATACCCAAAAACTTCATTGAAAGCATTGAAAATGGAAAGTGGATGAAGACATTCTCAGGTTATAAATCTCCACGAGCGGTGATTTTTCCAGATGAAACAGGAAAAGTTCTTTTTGATTTGATGAAGCATGTTCTGGAGGATGTTGCTATTTTGGACCAAGAATTTTATATGAATAAAATCAAACTTTACCAAGATGAACTGAAATTTCTTGGTGTGCGGCTTGGATCTGAGAATATAGGGAAGCTGGTCACCACTCGCTTTTTGTCTCTCGCATCTTCTGGAATGAGCAAAGAATCCACATATTCTTTGCTAACTTTCATTAGCTTCTCTAGAGGGAGGAGCATGGTTGATAATGGTTGGTTGGCTGTTATGAAGGAGAAGAAGTGGCTGAAGACGCTCCGAGGTTACAATCCTCCCAACGGATCTATTCTTTTGCTGCAATCTGAGATAGAAGTTGAAAACTGTTCAAAGGTTACAAATCTTCCAATTGTTGATGAAGCATTCTATGGAAGCAGATTAGGTTCATTCTTATCTGAGTTGAGGCTACTTGGAGTGACATATGATGCGGATGTGCTCCAAAGATTGGTTGCAGAGAACGTAAATCTAACTTCAAATCTGTCTTCTATGACTGGTGGTTGTGGTTTGCTGATTCTTAAATGTGTTAGATGGTTGGGATCTGGAGCTGAAGGCTTGATTAACAGGATTAAATGCCATCCTTGGGTAAAGACAACTCTAGGTTTCAAAAGCCCTTCGGAAACTGTTCTTCCTGATCCTAAGTGGGTTTCTTTGCTCAGTACTCTTCAAGTCCCTGCCATAGATGAATTGTATTATGACAATGCAATTAGGCAATTTGTTGATGAATTGAATGCATTGGGAGTGGTAGTTGATAACACTGGAACAACTGTGATGATTGCTGCTCGTTTTAAATCCCTATTGTCTTCCTCTAGCTTAGATCCTGCCACTGTTATGGCGCTGTTACGTTGCATCAGAGAAATGAATCTAACTATGTCTTTACAACACTCTGAACTGCAGTGGCTGCTGTCTGAAAAATGGTTAAAAACTCGGCATGGATTCAAGACTCCTGATGAATCAATTATTTTCAGCTCTAAATGGGGACAATTGATGTTGTTTGTTGATCTTCCTCTAATTGATGACGTGTACTATGGTACTGGCATATATAAGTTTAGGGATGAGCTAGAGAAGTTGGGTGCCATCACCGATTTTGAACGAGGAGCTCCGTTTGTAGCTAAAGGTCTACACAGTCCTATTGAAGCTGAGCTACTTGATGCAGATGGTATTAGATCATTGCTAGAGTGCATAAAGTCTCTGATGTCCCGATCGCTTGATGATCCCTTAGTGGGAGATTTTCTGAAGAACATTGCAAACAGCAGGTGTATGAAGACCACAAACGGTTATAAAGTGCCAGAAGACTGCATTCTATTTGATTCATCCTGGGAATGCATTCTGAACAGGTTGGATGCACCAAGCATTGATGAAGCATTTTACAGAACTGACATTTCTGCTTACAAGAATCAATTGAGAGATGTTGGTGTGAAGGTAGATCCTCAGGATGTTTGTTCTCTTCTTTCTGGGCATCTTTTGTCACTGACAGACACAGCTTCAATAACAAGGATATATAGCTTCCTCAACAAGTTCCAGTGGAGTCCTAAAGTTCTAAATAAGTGCAACTCTCAGGTGTGGATTCCTGAACCAAAGAGTAAGGCAGGGGCATGGGCAAAATCTCAATGTTGTGTACTTCATGATAAGAAAAATCTGTTTGGCTCTTGTTTGTTCAGTCTGGACAAATCTTACAGTAAAGAGCTTCTCCCCATTTTTTCTACAGCCTTTGGAGTTCCTGAAAATCCATCTATCAAGGATTACTTGCTGCTATGGGACATTTGGGCCTCAATGAACAACTCTAAAGTGACAGTTGAAAAATGCAGCTCGTTTTGGAAATGTATTGTTGACAACTGGAATCCACACATTGAAGAGACCCTAAAACAGAACTTAACAAAAGTACCTGCCACAATGTCCATGTCGGGGCAAATATATCTGATCAGAAAAGAGGAAGCATTCATTGCTGATGATTTGCAACTGAAAACAATTTTTTCGAGCTTTGATAAGGCACCTCTGTTTGTCTGGTTTCCCAATAGCTCGTCTTCTGTTCCTCATAGAAGACTTCTTGAAATTTATGAATCTCTTGGAGTTAGAAAGATCTCTGCATCTGTTAAGTGCACTGTGAAGGGCATGCTATCATCAGAGCATTGGGAAAAGGTCGAACAAAATAATGGATTGATTGGCAGGAGTTTGACCAAAATTGTTCTGGCTTTTCTTGCTGGTCCTGAGCTGAATATGCCGTTCAAAGAGAGACTTGAGGCTGCAAAATCGATGAGTGTACTGACAGTGTATAAGACTGACAAACCAATTAAAGTCTGTTATCGGCTAATGCCATGCGCAAGTACAACAGTGGAAGTGGAAAAACTGAAGCTGGTTCTTTGGGAGAAGGATTCTAAGTGGCTGTTCATTGATAAATCAGGTTATGATGGAGGAAAATGTGATTTAGAATTCGTTACTTCTTTTGCCAATGAACTGGCACAAGGACTACTAGCACAGGCAAGGCCCGCAGCTGCACATGCATTAAGCAGAATTATCCAAATGGCATACATATATGGTTTTAATGAGAATGAAGTGGAGTTTTTGCTGATGACTGAAAACTTAGAGTTATTTGTGGAAGATAAGAAGTTTCTTGATGATGCATATCCTGTTAGCCAAAAGCGCACAAGTAAGAAGCTAGAGCAATTAGGTCCCTCAACACCAATGCCTACCTGCAAGAAGCAGCGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1752

Amino Acids

198.04

Weight (kDa)

7.83

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 46 - 150 3.2e-10 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2475, 3828
AarI CACCTGC 1 cut(s) 3792
AatII GACGTC 1 cut(s) 364
Acc16I TGCGCA 2 cut(s) 2396, 4867
Acc36I ACCTGC 3 cut(s) 3792, 4441, 5246
AccB1I GGYRCC 2 cut(s) 3620, 4537
AccB7I CCANNNNNTGG 1 cut(s) 2995
AccBSI CCGCTC 1 cut(s) 2489
AccI GTMKAC 3 cut(s) 1557, 1640, 3667
AccII CGCG 2 cut(s) 1060, 1062
AciI CCGC 6 cut(s) 682, 1379, 2489, 2629, 2978, 5034
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 9 cut(s) 455, 1098, 1349, 2237, 2404, 2573, 2615, 4599, 4976
AcuI CTGAAG 5 cut(s) 201, 2822, 3117, 3803, 4916
AcyI GRCGYC 1 cut(s) 361
AfiI CCNNNNNNNGG 7 cut(s) 351, 2132, 2831, 2995, 3130, 3770, 4452
AflII CTTAAG 1 cut(s) 1634
AflIII ACRYGT 2 cut(s) 1327, 3567
AjiI CACGTC 1 cut(s) 3568
AjnI CCWGG 5 cut(s) 302, 474, 1359, 1719, 3863
AjuI GAANNNNNNNTTGG 2 cut(s) 1412, 1444
Alw21I GWGCWC 6 cut(s) 168, 262, 631, 2988, 3649, 4649
Alw26I GTCTC 9 cut(s) 352, 600, 730, 975, 1613, 2682, 3741, 4397, 4774
Alw44I GTGCAC 1 cut(s) 4645
AlwNI CAGNNNCTG 1 cut(s) 3448
Ama87I CYCGRG 1 cut(s) 2350
AoxI GGCC 7 cut(s) 334, 1662, 1717, 2145, 2361, 4314, 5030
ApaLI GTGCAC 1 cut(s) 4645
ApoI RAATTY 9 cut(s) 455, 1098, 1349, 2237, 2404, 2573, 2615, 4599, 4976
AseI ATTAAT 1 cut(s) 536
Asp700I GAANNNNTTC 3 cut(s) 2462, 3171, 3873
AspLEI GCGC 5 cut(s) 1062, 2397, 3385, 4868, 5190
AsuC2I CCSGG 1 cut(s) 650
AsuHPI GGTGA 8 cut(s) 95, 934, 1310, 1811, 2233, 2503, 2653, 3619
AvaI CYCGRG 1 cut(s) 2350
AvaII GGWCC 6 cut(s) 1242, 1357, 2122, 2566, 4751, 5216
AxyI CCTNAGG 1 cut(s) 3978
BaeGI GKGCMC 3 cut(s) 561, 1281, 4649
BaeI ACNNNNGTAYC 2 cut(s) 4413, 4446
BanI GGYRCC 2 cut(s) 3620, 4537
BanII GRGCYC 2 cut(s) 168, 3649
BarI GAAGNNNNNNTAC 2 cut(s) 3675, 3707
BauI CACGAG 2 cut(s) 789, 2484
BbsI GAAGAC 9 cut(s) 189, 2465, 2810, 3024, 3342, 3818, 3846, 4563, 4594
Bbv12I GWGCWC 6 cut(s) 168, 262, 631, 2988, 3649, 4649
BceAI ACGGC 3 cut(s) 321, 577, 4753
BciT130I CCWGG 5 cut(s) 304, 476, 1361, 1721, 3865
BclI TGATCA 1 cut(s) 4467
BcnI CCSGG 1 cut(s) 650
BcoDI GTCTC 9 cut(s) 352, 600, 730, 975, 1613, 2682, 3741, 4397, 4774
BfaI CTAG 9 cut(s) 1254, 1478, 2742, 3146, 3357, 3608, 3722, 5018, 5204
BfmI CTRYAG 5 cut(s) 126, 519, 1683, 3440, 4251
BfoI RGCGCY 1 cut(s) 3386
BfrI CTTAAG 1 cut(s) 1634
BfuAI ACCTGC 3 cut(s) 3792, 4441, 5246
BglII AGATCT 1 cut(s) 4626
BlpI GCTNAGC 2 cut(s) 1739, 3687
BmcAI AGTACT 1 cut(s) 3208
Bme1390I CCNGG 6 cut(s) 304, 476, 650, 1361, 1721, 3865
Bme18I GGWCC 6 cut(s) 1242, 1357, 2122, 2566, 4751, 5216
BmeT110I CYCGRG 1 cut(s) 2350
BmgBI CACGTC 1 cut(s) 3568
BmiI GGNNCC 6 cut(s) 1243, 1358, 2124, 3622, 4539, 5218
BmrFI CCNGG 6 cut(s) 304, 476, 650, 1361, 1721, 3865
BmrI ACTGGG 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 258
BoxI GACNNNNGTC 1 cut(s) 2307
BpiI GAAGAC 9 cut(s) 189, 2465, 2810, 3024, 3342, 3818, 3846, 4563, 4594
BplI GAGNNNNNCTC 2 cut(s) 1589, 1621
BpmI CTGGAG 6 cut(s) 239, 276, 1742, 1929, 2567, 3111
Bpu10I CCTNAGC 1 cut(s) 4755
Bpu1102I GCTNAGC 2 cut(s) 1739, 3687
BpuEI CTTGAG 2 cut(s) 1682, 4805
BpuMI CCSGG 1 cut(s) 650
Bsa29I ATCGAT 1 cut(s) 4799
BsaAI YACGTR 1 cut(s) 695
BsaBI GATNNNNATC 1 cut(s) 5169
BsaHI GRCGYC 1 cut(s) 361
BsaI GGTCTC 2 cut(s) 1613, 4397
BsaJI CCNNGG 4 cut(s) 303, 2812, 3128, 3864
BsaXI ACNNNNNCTCC 4 cut(s) 2780, 2810, 3279, 3309
Bsc4I CCNNNNNNNGG 7 cut(s) 351, 2132, 2831, 2995, 3130, 3770, 4452
Bse118I RCCGGY 1 cut(s) 262
Bse21I CCTNAGG 1 cut(s) 3978
Bse3DI GCAATG 5 cut(s) 445, 1141, 3716, 3789, 4488
Bse8I GATNNNNATC 1 cut(s) 5169
BseBI CCWGG 5 cut(s) 304, 476, 1361, 1721, 3865
BseCI ATCGAT 1 cut(s) 4799
BseDI CCNNGG 4 cut(s) 303, 2812, 3128, 3864
BseJI GATNNNNATC 1 cut(s) 5169
BseLI CCNNNNNNNGG 7 cut(s) 351, 2132, 2831, 2995, 3130, 3770, 4452
BseMI GCAATG 5 cut(s) 445, 1141, 3716, 3789, 4488
BseRI GAGGAG 3 cut(s) 878, 2764, 3657
BseSI GKGCMC 3 cut(s) 561, 1281, 4649
BseYI CCCAGC 1 cut(s) 11
BsgI GTGCAG 2 cut(s) 629, 5025
Bsh1236I CGCG 2 cut(s) 1060, 1062
Bsh1285I CGRYCG 1 cut(s) 3752
BshFI GGCC 7 cut(s) 336, 1664, 1719, 2147, 2363, 4316, 5032
BshNI GGYRCC 2 cut(s) 3620, 4537
BshVI ATCGAT 1 cut(s) 4799
BsiEI CGRYCG 1 cut(s) 3752
BsiHKAI GWGCWC 6 cut(s) 168, 262, 631, 2988, 3649, 4649
BsiHKCI CYCGRG 1 cut(s) 2350
BsiSI CCGG 3 cut(s) 263, 650, 1661
BslI CCNNNNNNNGG 7 cut(s) 351, 2132, 2831, 2995, 3130, 3770, 4452
BsmAI GTCTC 9 cut(s) 352, 600, 730, 975, 1613, 2682, 3741, 4397, 4774
BsmBI CGTCTC 2 cut(s) 352, 975
BsmI GAATGC 8 cut(s) 181, 1135, 2912, 3283, 3846, 3873, 3875, 4484
BsnI GGCC 7 cut(s) 336, 1664, 1719, 2147, 2363, 4316, 5032
Bso31I GGTCTC 2 cut(s) 1613, 4397
BsoBI CYCGRG 1 cut(s) 2350
Bsp1286I GDGCHC 8 cut(s) 168, 262, 561, 631, 1281, 2988, 3649, 4649
Bsp1407I TGTACA 1 cut(s) 1670
Bsp1720I GCTNAGC 2 cut(s) 1739, 3687
BspACI CCGC 6 cut(s) 682, 1379, 2489, 2629, 2978, 5034
BspANI GGCC 7 cut(s) 336, 1664, 1719, 2147, 2363, 4316, 5032
BspDI ATCGAT 1 cut(s) 4799
BspFNI CGCG 2 cut(s) 1060, 1062
BspHI TCATGA 2 cut(s) 2341, 4171
BspLI GGNNCC 6 cut(s) 1243, 1358, 2124, 3622, 4539, 5218
BspMAI CTGCAG 3 cut(s) 130, 523, 3444
BspMI ACCTGC 3 cut(s) 3792, 4441, 5246
BspT107I GGYRCC 2 cut(s) 3620, 4537
BspTI CTTAAG 1 cut(s) 1634
BspTNI GGTCTC 2 cut(s) 1613, 4397
BsrBI CCGCTC 1 cut(s) 2489
BsrDI GCAATG 5 cut(s) 445, 1141, 3716, 3789, 4488
BsrFI RCCGGY 1 cut(s) 262
BsrGI TGTACA 1 cut(s) 1670
BssAI RCCGGY 1 cut(s) 262
BssECI CCNNGG 4 cut(s) 303, 2812, 3128, 3864
BssNAI GTATAC 1 cut(s) 1641
BssNI GRCGYC 1 cut(s) 361
BssSI CACGAG 2 cut(s) 789, 2484
BssT1I CCWWGG 1 cut(s) 3128
Bst1107I GTATAC 1 cut(s) 1641
Bst2BI CACGAG 2 cut(s) 789, 2484
Bst2UI CCWGG 5 cut(s) 304, 476, 1361, 1721, 3865
Bst6I CTCTTC 9 cut(s) 33, 350, 955, 1415, 1635, 1683, 3216, 3999, 4395
BstACI GRCGYC 1 cut(s) 361
BstAFI CTTAAG 1 cut(s) 1634
BstAPI GCANNNNNTGC 2 cut(s) 3448, 4490
BstAUI TGTACA 1 cut(s) 1670
BstBAI YACGTR 1 cut(s) 695
BstC8I GCNNGC 4 cut(s) 1516, 2361, 4661, 5034
BstEII GGTNACC 1 cut(s) 2659
BstENI CCTNNNNNAGG 1 cut(s) 2130
BstFNI CGCG 2 cut(s) 1060, 1062
BstH2I RGCGCY 1 cut(s) 3386
BstHHI GCGC 5 cut(s) 1062, 2397, 3385, 4868, 5190
BstMAI GTCTC 9 cut(s) 352, 600, 730, 975, 1613, 2682, 3741, 4397, 4774
BstMCI CGRYCG 1 cut(s) 3752
BstNI CCWGG 5 cut(s) 304, 476, 1361, 1721, 3865
BstNSI RCATGY 4 cut(s) 1331, 2543, 4663, 5048
BstPAI GACNNNNGTC 1 cut(s) 2307
BstPI GGTNACC 1 cut(s) 2659
BstSCI CCNGG 6 cut(s) 302, 474, 648, 1359, 1719, 3863
BstSFI CTRYAG 5 cut(s) 126, 519, 1683, 3440, 4251
BstSLI GKGCMC 3 cut(s) 561, 1281, 4649
BstUI CGCG 2 cut(s) 1060, 1062
BstV2I GAAGAC 9 cut(s) 189, 2465, 2810, 3024, 3342, 3818, 3846, 4563, 4594
BstX2I RGATCY 6 cut(s) 2636, 2834, 3086, 3364, 3973, 4626
BstXI CCANNNNNNTGG 4 cut(s) 482, 1075, 3379, 5002
BstYI RGATCY 6 cut(s) 2636, 2834, 3086, 3364, 3973, 4626
BstZ17I GTATAC 1 cut(s) 1641
Bsu15I ATCGAT 1 cut(s) 4799
Bsu36I CCTNAGG 1 cut(s) 3978
BsuRI GGCC 7 cut(s) 336, 1664, 1719, 2147, 2363, 4316, 5032
BsuTUI ATCGAT 1 cut(s) 4799
BtrI CACGTC 1 cut(s) 3568
BtsI GCAGTG 1 cut(s) 3449
BveI ACCTGC 3 cut(s) 3792, 4441, 5246
Cac8I GCNNGC 4 cut(s) 1516, 2361, 4661, 5034
CaiI CAGNNNCTG 1 cut(s) 3448
CciI TCATGA 2 cut(s) 2341, 4171
CfoI GCGC 5 cut(s) 1062, 2397, 3385, 4868, 5190
Cfr10I RCCGGY 1 cut(s) 262
ClaI ATCGAT 1 cut(s) 4799
CseI GACGC 1 cut(s) 2815
CsiI ACCWGGT 2 cut(s) 474, 1359
DraI TTTAAA 1 cut(s) 3337
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 9 cut(s) 33, 350, 955, 1415, 1635, 1683, 3216, 3999, 4395
EarI CTCTTC 9 cut(s) 33, 350, 955, 1415, 1635, 1683, 3216, 3999, 4395
Ecl136II GAGCTC 2 cut(s) 166, 3647
Eco130I CCWWGG 1 cut(s) 3128
Eco147I AGGCCT 1 cut(s) 2363
Eco24I GRGCYC 2 cut(s) 168, 3649
Eco31I GGTCTC 2 cut(s) 1613, 4397
Eco32I GATATC 1 cut(s) 446
Eco47I GGWCC 6 cut(s) 1242, 1357, 2122, 2566, 4751, 5216
Eco53kI GAGCTC 2 cut(s) 166, 3647
Eco57I CTGAAG 5 cut(s) 201, 2822, 3117, 3803, 4916
Eco81I CCTNAGG 1 cut(s) 3978
Eco88I CYCGRG 1 cut(s) 2350
Eco91I GGTNACC 1 cut(s) 2659
EcoICRI GAGCTC 2 cut(s) 166, 3647
EcoNI CCTNNNNNAGG 1 cut(s) 2130
EcoO109I RGGNCCY 2 cut(s) 1357, 5216
EcoO65I GGTNACC 1 cut(s) 2659
EcoRI GAATTC 3 cut(s) 1098, 2404, 4976
EcoRII CCWGG 5 cut(s) 302, 474, 1359, 1719, 3863
EcoRV GATATC 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 3128
EcoT22I ATGCAT 5 cut(s) 1135, 3283, 3875, 5050, 5170
EcoT38I GRGCYC 2 cut(s) 168, 3649
ErhI CCWWGG 1 cut(s) 3128
Esp3I CGTCTC 2 cut(s) 352, 975
FauI CCCGC 1 cut(s) 5041
FauNDI CATATG 3 cut(s) 1489, 1930, 2971
FbaI TGATCA 1 cut(s) 4467
FblI GTMKAC 3 cut(s) 1557, 1640, 3667
FriOI GRGCYC 2 cut(s) 168, 3649
FspBI CTAG 9 cut(s) 1254, 1478, 2742, 3146, 3357, 3608, 3722, 5018, 5204
FspI TGCGCA 2 cut(s) 2396, 4867
GlaI GCGC 5 cut(s) 1061, 2396, 3384, 4867, 5189
GsaI CCCAGC 1 cut(s) 15
GsuI CTGGAG 6 cut(s) 239, 276, 1742, 1929, 2567, 3111
HaeII RGCGCY 1 cut(s) 3386
HaeIII GGCC 7 cut(s) 336, 1664, 1719, 2147, 2363, 4316, 5032
HapII CCGG 3 cut(s) 263, 650, 1661
HgaI GACGC 1 cut(s) 2815
HhaI GCGC 5 cut(s) 1062, 2397, 3385, 4868, 5190
Hin1I GRCGYC 1 cut(s) 361
Hin6I GCGC 5 cut(s) 1060, 2395, 3383, 4866, 5188
HinP1I GCGC 5 cut(s) 1060, 2395, 3383, 4866, 5188
HincII GTYRAC 1 cut(s) 4378
HindII GTYRAC 1 cut(s) 4378
HpaII CCGG 3 cut(s) 263, 650, 1661
HphI GGTGA 8 cut(s) 95, 934, 1310, 1811, 2233, 2503, 2653, 3619
Hpy99I CGWCG 1 cut(s) 1848
HpyCH4IV ACGT 7 cut(s) 361, 694, 968, 1846, 3009, 3391, 3567
HpySE526I ACGT 7 cut(s) 361, 694, 968, 1846, 3009, 3391, 3567
Hsp92I GRCGYC 1 cut(s) 361
HspAI GCGC 5 cut(s) 1060, 2395, 3383, 4866, 5188
Ksp22I TGATCA 1 cut(s) 4467
MabI ACCWGGT 2 cut(s) 474, 1359
MaeI CTAG 9 cut(s) 1254, 1478, 2742, 3146, 3357, 3608, 3722, 5018, 5204
MaeII ACGT 7 cut(s) 361, 694, 968, 1846, 3009, 3391, 3567
MbiI CCGCTC 1 cut(s) 2489
MfeI CAATTG 6 cut(s) 1154, 1888, 2073, 2898, 3530, 3947
MflI RGATCY 6 cut(s) 2636, 2834, 3086, 3364, 3973, 4626
MhlI GDGCHC 8 cut(s) 168, 262, 561, 631, 1281, 2988, 3649, 4649
MlyI GAGTC 6 cut(s) 208, 311, 1598, 1666, 3484, 4084
MmeI TCCRAC 2 cut(s) 618, 3867
Mph1103I ATGCAT 5 cut(s) 1135, 3283, 3875, 5050, 5170
MroXI GAANNNNTTC 3 cut(s) 2462, 3171, 3873
MslI CAYNNNNRTG 1 cut(s) 3377
MspA1I CMGCKG 1 cut(s) 5039
MspCI CTTAAG 1 cut(s) 1634
MspI CCGG 3 cut(s) 263, 650, 1661
MspR9I CCNGG 6 cut(s) 304, 476, 650, 1361, 1721, 3865
MunI CAATTG 6 cut(s) 1154, 1888, 2073, 2898, 3530, 3947
Mva1269I GAATGC 8 cut(s) 181, 1135, 2912, 3283, 3846, 3873, 3875, 4484
MvaI CCWGG 5 cut(s) 304, 476, 1361, 1721, 3865
MvnI CGCG 2 cut(s) 1060, 1062
NciI CCSGG 1 cut(s) 650
NdeI CATATG 3 cut(s) 1489, 1930, 2971
NlaIV GGNNCC 6 cut(s) 1243, 1358, 2124, 3622, 4539, 5218
NmeAIII GCCGAG 1 cut(s) 3454
NmuCI GTSAC 6 cut(s) 112, 1009, 2659, 2965, 4014, 4336
NsbI TGCGCA 2 cut(s) 2396, 4867
NsiI ATGCAT 5 cut(s) 1135, 3283, 3875, 5050, 5170
NspI RCATGY 4 cut(s) 1331, 2543, 4663, 5048
PaeI GCATGC 1 cut(s) 4663
PaeR7I CTCGAG 1 cut(s) 2350
PagI TCATGA 2 cut(s) 2341, 4171
PaqCI CACCTGC 1 cut(s) 3792
PceI AGGCCT 1 cut(s) 2363
PciI ACATGT 1 cut(s) 1327
PctI GAATGC 8 cut(s) 181, 1135, 2912, 3283, 3846, 3873, 3875, 4484
PdmI GAANNNNTTC 3 cut(s) 2462, 3171, 3873
PflMI CCANNNNNTGG 1 cut(s) 2995
Ple19I CGATCG 1 cut(s) 3752
PleI GAGTC 6 cut(s) 208, 311, 1598, 1665, 3484, 4083
PpsI GAGTC 6 cut(s) 208, 311, 1598, 1665, 3484, 4083
Ppu21I YACGTR 1 cut(s) 695
PpuMI RGGWCCY 2 cut(s) 1357, 5216
PscI ACATGT 1 cut(s) 1327
PshAI GACNNNNGTC 1 cut(s) 2307
PshBI ATTAAT 1 cut(s) 536
PsiI TTATAA 2 cut(s) 2475, 3828
Psp124BI GAGCTC 2 cut(s) 168, 3649
Psp5II RGGWCCY 2 cut(s) 1357, 5216
Psp6I CCWGG 5 cut(s) 302, 474, 1359, 1719, 3863
PspEI GGTNACC 1 cut(s) 2659
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 5 cut(s) 302, 474, 1359, 1719, 3863
PspN4I GGNNCC 6 cut(s) 1243, 1358, 2124, 3622, 4539, 5218
PspPPI RGGWCCY 2 cut(s) 1357, 5216
PsrI GAACNNNNNNTAC 2 cut(s) 525, 557
PstI CTGCAG 3 cut(s) 130, 523, 3444
PstNI CAGNNNCTG 1 cut(s) 3448
PsuI RGATCY 6 cut(s) 2636, 2834, 3086, 3364, 3973, 4626
PvuI CGATCG 1 cut(s) 3752
PvuII CAGCTG 1 cut(s) 5039
RseI CAYNNNNRTG 1 cut(s) 3377
SacI GAGCTC 2 cut(s) 168, 3649
ScaI AGTACT 1 cut(s) 3208
SchI GAGTC 6 cut(s) 208, 311, 1598, 1666, 3484, 4084
ScrFI CCNGG 6 cut(s) 304, 476, 650, 1361, 1721, 3865
SduI GDGCHC 8 cut(s) 168, 262, 561, 631, 1281, 2988, 3649, 4649
SexAI ACCWGGT 2 cut(s) 474, 1359
SfcI CTRYAG 5 cut(s) 126, 519, 1683, 3440, 4251
Sfr274I CTCGAG 1 cut(s) 2350
SinI GGWCC 6 cut(s) 1242, 1357, 2122, 2566, 4751, 5216
SlaI CTCGAG 1 cut(s) 2350
SmiMI CAYNNNNRTG 1 cut(s) 3377
SmlI CTYRAG 4 cut(s) 1634, 1697, 2350, 4784
SmoI CTYRAG 4 cut(s) 1634, 1697, 2350, 4784
SphI GCATGC 1 cut(s) 4663
SseBI AGGCCT 1 cut(s) 2363
SsiI CCGC 6 cut(s) 682, 1379, 2489, 2629, 2978, 5034
SspMI CTAG 9 cut(s) 1254, 1478, 2742, 3146, 3357, 3608, 3722, 5018, 5204
SstI GAGCTC 2 cut(s) 168, 3649
StuI AGGCCT 1 cut(s) 2363
StyD4I CCNGG 6 cut(s) 302, 474, 648, 1359, 1719, 3863
StyI CCWWGG 1 cut(s) 3128
TaiI ACGT 7 cut(s) 364, 697, 971, 1849, 3012, 3394, 3570
TaqI TCGA 7 cut(s) 1324, 1705, 1964, 2351, 4523, 4689, 4799
TaqII GACCGA 1 cut(s) 248
TatI WGTACW 7 cut(s) 192, 1670, 3206, 3570, 4165, 4807, 4871
TauI GCSGC 2 cut(s) 685, 2632
TseFI GTSAC 6 cut(s) 112, 1009, 2659, 2965, 4014, 4336
Tsp45I GTSAC 6 cut(s) 112, 1009, 2659, 2965, 4014, 4336
TspGWI ACGGA 3 cut(s) 2306, 2847, 3639
Van91I CCANNNNNTGG 1 cut(s) 2995
Vha464I CTTAAG 1 cut(s) 1634
VneI GTGCAC 1 cut(s) 4645
VpaK11BI GGWCC 6 cut(s) 1242, 1357, 2122, 2566, 4751, 5216
VspI ATTAAT 1 cut(s) 536
XagI CCTNNNNNAGG 1 cut(s) 2130
XapI RAATTY 9 cut(s) 455, 1098, 1349, 2237, 2404, 2573, 2615, 4599, 4976
XbaI TCTAGA 1 cut(s) 2741
XceI RCATGY 4 cut(s) 1331, 2543, 4663, 5048
XcmI CCANNNNNNNNNTGG 1 cut(s) 4732
XhoI CTCGAG 1 cut(s) 2350
XmiI GTMKAC 3 cut(s) 1557, 1640, 3667
XmnI GAANNNNTTC 3 cut(s) 2462, 3171, 3873
XspI CTAG 9 cut(s) 1254, 1478, 2742, 3146, 3357, 3608, 3722, 5018, 5204
ZraI GACGTC 1 cut(s) 362
ZrmI AGTACT 1 cut(s) 3208
Zsp2I ATGCAT 5 cut(s) 1135, 3283, 3875, 5050, 5170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.