RchiOBHm_Chr4g0412151

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003946
Physical Location & Seq
Reverse (-)
35895097 .. 35902117
7021 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38289

Sequence Viewer

Length: 5262 bp
ATGGGAAATCCCAGCATTGAAGCCATGCAACACATAGAAGAGATCAGGACAAACAAGTTCTCAATAGGGAAGAAAGAACCAAACCCCTTAACTCTTGATCTTCACCATGCTGTCACCAGTCTATCTGCAGAGCTGTATCAGAAAGACATCCATTTCCTGATGGAGCTCATACAGAATGCTGAAGACAATGAGTACAAAGAAGGTGTAGAACCGACTCTGGAGTTTGTGCTGACCGAGAAAGACATAACTGGGACTGGAGCACCGGCTACTCTTTTGGTTTTCAATAATGAGGTTGGCTTTTCCAGGGAGAATATAGACTCCATTTGCAGCATTGGCCGTTCTACCAAGAAAGGGAAGAGACGTCAGGGCTTTATTGGAGAGAAAGGAATTGGATTCAAAAGTGTGTTTCTTGTGAGTTCACAACCTCACATATTTAGCAATGGATATCATGTCAAATTCAGGGAAGAACCAAACCAGGTCTGTGGTATTGGTTATATAGTTCCTGAATGGTTTAGGGCTGCAGATTTGTCAGTATTAATGGTTCAGGCTGTGTATGGGCACGGCAAGGACTTGCCAACAACTACGTTCGTACTTCCTCTAAAGCGAGACAAGGTGGAAGCAGTGAGAGCACAACTGTTGGAGCTGCACCCGGAGATTCTCTTGTTCTTATCCAAAATAAAGCGGCTATATGTACGTGGGTGTGATCCTCAAAAAGCTGATGATGTCTCAACATTATCTATATTTAGTGAGACTGAACATACAGAGTTAAGGAGTAAAACAGCCAATTCTCGTGTCGTGCAACTTTCTGTTAAGGAAAAAATGTGTGATACTGAGCAGTTGTGCAAGTATTACTTGTGGAGAGAGGAGTTTCCAGTGAAACCTGGGAATAGAGTTGAAATAAGGATGGATGTGGAGAAATGGGTGATTACTCTTGCTTTCCCATTTGGAAAAAGGCTGAGAAGAGGAACGTCTCCTGTTGGTATATTTGCTTTCCTGCCAACAACAATGGTCACAAACTTCCCCTTTGTAGTTCAAGCTGACTTCATCCTTGCTTCTTCGCGCGAATCCATAATTTTGGACAATTCATGGAACATGGGAATTCTTCAATGTGTCCCATCAGCCTTTGTGAATGCATTTCAACATTGCGTGGGACAACTGAACCTCTTTTCTTCTGTAAGTCAGCCATTTGAGTTTTTACCTGCTCAAAACTCGCCAACACCAGAGTTTAATACAGTACAAGAGTCCATTAGGACTAGGCTGCAAGGTCTAAACATAGTGCCCTGTAAAAGATTTTTTGGTGATAGTCTTTCCTCCAAGCTGCCTCGACGTGTAGTACGGATCAAGCCAAAATTTAGGGACCTGGTTGTTAAAATTAAAGCGGAAGGTGCTGTTTTTGGCAGTTCATCTTCATCTTCATTGAAGAGGGTCTTGGATTCATCTCTGGATGTTGAAAAATACAGGGACATTATGAACTTTCTAGGTGTGGCATATGCTGATGATGATTGGTACACAGCCTGCATCAACTCTTGTAATCTTTTGTTGTTATCAGATGAAGTCTACATTGAACTACTTGGTTTTTTGGCTGATAATGATGCAGGGTTTGACTCAGATAGCATTGAGACCATACCTCTTCTTAAGTATACTGATCGGAAAGGGAGTCTGGCCTTGTGTACAATTAAAACTACAGAAGTGGAACTCAAGATTCGATATGCTATGGGCCTGGAGCTTCACACATGGCTCAGCAAGTGCAATATGGAGTTTGGCTGTCCTGATAATGTGTATTTCTTACCAAATAAGACACTGGAAGCTGTTTTGAATCACCAGAAAAGTCATCCCCCAAAGCGACGTTATAGAAAGAAAAATTACATGGAGAGCTCATCTGTCTGCAATTGGCTGAATAGAAGTGCTGGAGTGAAATCTCTTTCAGCATATGATTATGCTTTCTTACTTTTGAATCATGTATCAAAGAAGGAATCCAATGTTGCAGTCAGATTATCCAATTTCCTTTATCTTGCCCACAAGGAAGGCTTCCTCACTGAATATCAAATATCTGATCTTTGTGGTAGGATTCCAATTGTTGATGGGTCTGATAAAGTAAGAGTGCAAAGAACCGTAACTTTGGTCCCTTCATCAGGGAGCAAATGGGCCAAATTGTTTGGACTTCAAAATCCATTTGCTGAACAACATTATGTTGATATAGGGGATGTTTATGCTAAAAGCAGTTTGTTTCTTGGGAAATATTCTACCCCTGAGAAAGAGCTTCTGTATTTTGTTGGCGAGTATAGCAAAGCTACGGACTTACCCGACTTGTGTCCTCCTGATGTGGTGTTACAGATCGCATCTCATGAACTCTCGAGTGAGCAGGCCTTTTTACTCCTTGATTGGATCAGGTTACTGCGCACCAAGAATTCATCCATACCCAAAAACTTCATTGAAAGCATTGAAAATGGAAAGTGGATGAAGACATTCTCAGGTTATAAATCTCCACAAGAGGTCATTTTTCCAGATGAAACAGGAAAAGTTATTTTTGATTTGATGAAGCATGTTCTGGAGGATGTTGCTATTTTGGACCAAGAATTTTATATGAATAAAATCAAACTTTACCAAGATGAACTGAAATTTCTTGGTGTGCGGCTTGGATCTGAGAATATAGGGAAGCTGGTCACCACTCGCTTTTTGTCTCTCGCATCTTCTGGAATGAGCAAAGAATCCACATATTCTTTGCTAACTTTCATTAGCTTCTCTAGAGGGAGGAGCATGGTTGATAATGGTTGGTTGGCTGTTATGAAGGAGAAGAAGTGGCTGAAGACGCTCCGAGGTTACAATCCTCCCAACGGATCTATTCTTTTGCTGCAATCTGAGATAGAAGTTGAAAACTGTTCAAAGGTTACAAATCTTCCGATTGTTGATGAAGCATTCTATGGAAGCAGATTAGGTTCATTCTTATCTGAGTTGAGGCTACTTGGAGTGACATATGATGCGGATGTGCTCCAAAGATTGGTTGCAGAGAACGTAAATCTAACTTCAAATCTGTCTTCTATGACTGGTGGTTGTGGTTTGCTGATTCTTAAATGTGTTAGATGGTTGGGATCTGGAGCTGCAGGCTTGATTAACAGGATAAAATGCCATCCTTGGGTAAAGACAACTCTAGGTTTCAAAAGCCCTTCGGAAACTGTTCTTCCTGATCCTAAGTGGGTTTCTTTGCTCAGTACTCTTCAAGTCCCTGCCATAGATGAATTGTATTATGACAATGCAATTAGGCAATTTGTTGATGAATTGAATGCATTGGGAGTGGTAGTTGATAACACTGGAACAACTGTGATGATAGCTGCTCGTTTTAAATCCCTATTGTCTTCCTCTAGCTTAGATCCTGCCACTGTTATGGCGCTGTTACGTTGCATCAGAGAAATGAATCTAACTATGTCTTTACAACACTCTGAACTGCAGTGGCTGCTGTCTGAAAAATGGTTAAAAACTCGGCATGGATTCAAGACTCCTGATGAATCAATTATTTTCAGCTCTAAATGGGGACAATTGATGTTGTTTGTTGATCTTCCTCTAATTGATGACGTGTACTATGGTACTGGCATATATAAGTTTAGGGATGAGCTAGAGAAGTTGGGTGCCATCACCGATTTTGAACGAGGAGCTCTATTTGTAGCTAAAGGTCTACACAGTCCTATTGAAGCTGAGCTACTTGATGCAGATGGTATTAGATCATTGCTAGAGTGCATAAAGTCTCTGATGTCCCGATCGCTTGATGATCCCTTAGTGGGAGATTTTCTGAAGAACATTGCAAACAGCAGGTGTTTGAAGACCACAAACGGTTATAAAGTGCCAGAAGACTGCATTCTATTTGATTCATCCTGGGAATGCATTCTGAACAGGTTGGATGCACCAAGCATTGATGAAGCATTTTACAGAACTGACATTTCTGCTTACAAGAATCAATTGAGAGATGTTGGTGTGAAGGTAGATCCTCAGGATGTTTGTTCTCTTCTTTCTGGTCATCTTTTGTCACTGACAGACACAGCTTCAATAACAAGGATATATAGCTTCCTCAACAAGTTCCAGTGGAGTCCTAAAGTTCTAAATAAGTGCAACTCTCAGGTGTGGATTCCTGAACCAAAGAGTAAGGCAGGGGCATGGGCAAAATCTCAATGTTGTGTACTTCATGATAAGAAAAATCTGTTTGGCTCTTGTTTGTTCAGTCTGGACAAATCTTACAGTAAAGAGCTTCTCCCCATTTTTTCTACAGCCTTTGGAGTTCCTGAAAATCCATCTATCAAGGATTACTTGCTGCTATGGGACATTTGGGCCTCAATGAACAACTCTAAAGTGACAGTTGAAAAATGCAGCTCGTTTTGGAAATGTATTGTTGACAACTGGAATCCACACATTGAAGAGACCCTAAAACAGAACTTAACAAAAGTACCTGCCACAATGTCCATGTCGGGGCAAATATATCTGATCAGAAAAGAGGAAGCATTCATTGCTGATGATTTGCAACTGAAAACAATTTTTTCGAGCTTTGATAAGGCACCTCTGTTTGTCTGGTTTCCTAATAGCTCGTCTTCTGTTCCTCATAGAAGACTTCTTGAAATTTATGAATCTCTTGGAGTTAGAAAGATCTCTGCATCTGTTAAGTGCACTGTGAAGGGCATGCTATCATCAGAGCATTGGGAAAAGGTCGAACAAAATAATGGATTGATTGGCAGGAGTTTGACCAAAATTGTTCTGGCTTTTCTTGCTGGTCCTGAGCTGAATATGCCGTTCAAAGAGAGACTTGAGGCTGCAAAATCGATGAGTGTGCTGACAGCGTATAAGACTGACAAACCAATTAAAGTCTGTTATCGGCTAATGCCATGCGCAAGTACAACAGTGGAAGTGGAAAAACTGAAGCTGGTTCTTTGGGAGAAGGATTCTAAGCGGCTGTTCATTGATAAATCAGGTTATGATGGAGGAAAATGTGATTTAGAATTCGTTACTTCTTTTGCCAATGAACTGGCACAAGAACTACTAGCACAGGCAAGACCCGCAGCTGCACATGCATTAAGCAGAATTATCCAAATGGCATACATATATGGTTTTAATGAGAATGAAGTGGAGTTTTTGCTGATGACTGAAAACTTAGAGTTATTTGTGGAAGATAAGAAGTTTCTTGATGATGCATATCCTGTTAGCCAAAAGCGCACAAGTAAGAAGCTAGAGCAATTAGGTCCCTCAACACCAATGCCTACCTGCAAGAAGCAGCGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1753

Amino Acids

197.97

Weight (kDa)

7.83

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 46 - 150 3.4e-10 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2478, 3831
AarI CACCTGC 1 cut(s) 3795
AatII GACGTC 1 cut(s) 364
Acc16I TGCGCA 2 cut(s) 2399, 4870
Acc36I ACCTGC 4 cut(s) 1207, 3795, 4444, 5249
AccB1I GGYRCC 2 cut(s) 3623, 4540
AccB7I CCANNNNNTGG 1 cut(s) 2998
AccI GTMKAC 3 cut(s) 1557, 1640, 3670
AccII CGCG 2 cut(s) 1060, 1062
AciI CCGC 6 cut(s) 682, 1379, 2632, 2981, 4930, 5037
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 8 cut(s) 455, 1098, 1349, 2407, 2576, 2618, 4602, 4979
AcuI CTGAAG 4 cut(s) 201, 2825, 3806, 4919
AcyI GRCGYC 1 cut(s) 361
AfiI CCNNNNNNNGG 7 cut(s) 351, 2132, 2834, 2998, 3133, 3773, 4455
AflII CTTAAG 1 cut(s) 1634
AflIII ACRYGT 2 cut(s) 1327, 3570
AjiI CACGTC 2 cut(s) 1328, 3571
AjnI CCWGG 6 cut(s) 302, 474, 880, 1359, 1719, 3866
AjuI GAANNNNNNNTTGG 2 cut(s) 1412, 1444
Alw21I GWGCWC 7 cut(s) 168, 262, 631, 1877, 2991, 3652, 4652
Alw44I GTGCAC 1 cut(s) 4648
AlwNI CAGNNNCTG 1 cut(s) 3451
Ama87I CYCGRG 1 cut(s) 2353
AoxI GGCC 6 cut(s) 334, 1662, 1717, 2145, 2364, 4317
ApaLI GTGCAC 1 cut(s) 4648
ApoI RAATTY 8 cut(s) 455, 1098, 1349, 2407, 2576, 2618, 4602, 4979
AseI ATTAAT 1 cut(s) 536
Asp700I GAANNNNTTC 3 cut(s) 2465, 3174, 3876
AspLEI GCGC 5 cut(s) 1062, 2400, 3388, 4871, 5193
AspS9I GGNCC 8 cut(s) 1357, 1717, 2122, 2145, 2569, 4317, 4754, 5219
AsuC2I CCSGG 1 cut(s) 650
AsuHPI GGTGA 7 cut(s) 95, 106, 934, 1310, 1811, 2656, 3622
AvaI CYCGRG 1 cut(s) 2353
AvaII GGWCC 5 cut(s) 1357, 2122, 2569, 4754, 5219
AxyI CCTNAGG 1 cut(s) 3981
BaeGI GKGCMC 3 cut(s) 561, 1281, 4652
BaeI ACNNNNGTAYC 2 cut(s) 4416, 4449
BanI GGYRCC 2 cut(s) 3623, 4540
BanII GRGCYC 3 cut(s) 168, 1877, 3652
BarI GAAGNNNNNNTAC 2 cut(s) 3678, 3710
BauI CACGAG 1 cut(s) 789
BbsI GAAGAC 9 cut(s) 189, 2468, 2813, 3027, 3345, 3821, 3849, 4566, 4597
Bbv12I GWGCWC 7 cut(s) 168, 262, 631, 1877, 2991, 3652, 4652
BceAI ACGGC 3 cut(s) 321, 577, 4756
BcgI CGANNNNNNTGC 2 cut(s) 4792, 4826
BciT130I CCWGG 6 cut(s) 304, 476, 882, 1361, 1721, 3868
BclI TGATCA 1 cut(s) 4470
BcnI CCSGG 1 cut(s) 650
BfaI CTAG 9 cut(s) 1254, 1478, 2745, 3149, 3360, 3611, 3725, 5021, 5207
BfmI CTRYAG 6 cut(s) 126, 519, 1683, 3099, 3443, 4254
BfoI RGCGCY 1 cut(s) 3389
BfrI CTTAAG 1 cut(s) 1634
BfuAI ACCTGC 4 cut(s) 1207, 3795, 4444, 5249
BglII AGATCT 1 cut(s) 4629
BlpI GCTNAGC 2 cut(s) 1739, 3690
BmcAI AGTACT 1 cut(s) 3211
Bme1390I CCNGG 7 cut(s) 304, 476, 650, 882, 1361, 1721, 3868
Bme18I GGWCC 5 cut(s) 1357, 2122, 2569, 4754, 5219
BmeT110I CYCGRG 1 cut(s) 2353
BmgBI CACGTC 2 cut(s) 1328, 3571
BmgT120I GGNCC 8 cut(s) 1357, 1717, 2122, 2145, 2569, 4317, 4754, 5219
BmiI GGNNCC 5 cut(s) 1358, 2124, 3625, 4542, 5221
BmrFI CCNGG 7 cut(s) 304, 476, 650, 882, 1361, 1721, 3868
BmrI ACTGGG 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 258
BoxI GACNNNNGTC 1 cut(s) 2310
BpiI GAAGAC 9 cut(s) 189, 2468, 2813, 3027, 3345, 3821, 3849, 4566, 4597
BpmI CTGGAG 6 cut(s) 239, 276, 1742, 1929, 2570, 3114
Bpu10I CCTNAGC 1 cut(s) 4758
Bpu1102I GCTNAGC 2 cut(s) 1739, 3690
BpuEI CTTGAG 2 cut(s) 1682, 4808
BpuMI CCSGG 1 cut(s) 650
Bsa29I ATCGAT 1 cut(s) 4802
BsaAI YACGTR 1 cut(s) 695
BsaBI GATNNNNATC 1 cut(s) 5172
BsaHI GRCGYC 1 cut(s) 361
BsaI GGTCTC 2 cut(s) 1613, 4400
BsaJI CCNNGG 5 cut(s) 303, 881, 2815, 3131, 3867
BsaXI ACNNNNNCTCC 4 cut(s) 2783, 2813, 3282, 3312
Bsc4I CCNNNNNNNGG 7 cut(s) 351, 2132, 2834, 2998, 3133, 3773, 4455
Bse118I RCCGGY 1 cut(s) 262
Bse21I CCTNAGG 1 cut(s) 3981
Bse3DI GCAATG 5 cut(s) 445, 1141, 3719, 3792, 4491
Bse8I GATNNNNATC 1 cut(s) 5172
BseBI CCWGG 6 cut(s) 304, 476, 882, 1361, 1721, 3868
BseCI ATCGAT 1 cut(s) 4802
BseDI CCNNGG 5 cut(s) 303, 881, 2815, 3131, 3867
BseJI GATNNNNATC 1 cut(s) 5172
BseLI CCNNNNNNNGG 7 cut(s) 351, 2132, 2834, 2998, 3133, 3773, 4455
BseMI GCAATG 5 cut(s) 445, 1141, 3719, 3792, 4491
BseRI GAGGAG 3 cut(s) 878, 2767, 3660
BseSI GKGCMC 3 cut(s) 561, 1281, 4652
BseYI CCCAGC 1 cut(s) 11
BsgI GTGCAG 2 cut(s) 629, 5028
Bsh1236I CGCG 2 cut(s) 1060, 1062
Bsh1285I CGRYCG 1 cut(s) 3755
BshFI GGCC 6 cut(s) 336, 1664, 1719, 2147, 2366, 4319
BshNI GGYRCC 2 cut(s) 3623, 4540
BshVI ATCGAT 1 cut(s) 4802
BsiEI CGRYCG 1 cut(s) 3755
BsiHKAI GWGCWC 7 cut(s) 168, 262, 631, 1877, 2991, 3652, 4652
BsiHKCI CYCGRG 1 cut(s) 2353
BsiSI CCGG 2 cut(s) 263, 650
BslI CCNNNNNNNGG 7 cut(s) 351, 2132, 2834, 2998, 3133, 3773, 4455
BsmBI CGTCTC 2 cut(s) 352, 975
BsmI GAATGC 8 cut(s) 181, 1135, 2915, 3286, 3849, 3876, 3878, 4487
BsnI GGCC 6 cut(s) 336, 1664, 1719, 2147, 2366, 4319
Bso31I GGTCTC 2 cut(s) 1613, 4400
BsoBI CYCGRG 1 cut(s) 2353
Bsp1286I GDGCHC 9 cut(s) 168, 262, 561, 631, 1281, 1877, 2991, 3652, 4652
Bsp1407I TGTACA 1 cut(s) 1670
Bsp1720I GCTNAGC 2 cut(s) 1739, 3690
BspACI CCGC 6 cut(s) 682, 1379, 2632, 2981, 4930, 5037
BspANI GGCC 6 cut(s) 336, 1664, 1719, 2147, 2366, 4319
BspDI ATCGAT 1 cut(s) 4802
BspFNI CGCG 2 cut(s) 1060, 1062
BspHI TCATGA 2 cut(s) 2344, 4174
BspLI GGNNCC 5 cut(s) 1358, 2124, 3625, 4542, 5221
BspMAI CTGCAG 4 cut(s) 130, 523, 3103, 3447
BspMI ACCTGC 4 cut(s) 1207, 3795, 4444, 5249
BspT107I GGYRCC 2 cut(s) 3623, 4540
BspTI CTTAAG 1 cut(s) 1634
BspTNI GGTCTC 2 cut(s) 1613, 4400
BsrDI GCAATG 5 cut(s) 445, 1141, 3719, 3792, 4491
BsrFI RCCGGY 1 cut(s) 262
BsrGI TGTACA 1 cut(s) 1670
BssAI RCCGGY 1 cut(s) 262
BssECI CCNNGG 5 cut(s) 303, 881, 2815, 3131, 3867
BssNAI GTATAC 1 cut(s) 1641
BssNI GRCGYC 1 cut(s) 361
BssSI CACGAG 1 cut(s) 789
BssT1I CCWWGG 1 cut(s) 3131
Bst1107I GTATAC 1 cut(s) 1641
Bst2BI CACGAG 1 cut(s) 789
Bst2UI CCWGG 6 cut(s) 304, 476, 882, 1361, 1721, 3868
Bst6I CTCTTC 8 cut(s) 33, 350, 955, 1415, 1635, 3219, 4002, 4398
BstACI GRCGYC 1 cut(s) 361
BstAFI CTTAAG 1 cut(s) 1634
BstAPI GCANNNNNTGC 2 cut(s) 3451, 4493
BstAUI TGTACA 1 cut(s) 1670
BstBAI YACGTR 1 cut(s) 695
BstC8I GCNNGC 4 cut(s) 1516, 2364, 3103, 4664
BstEII GGTNACC 1 cut(s) 2662
BstENI CCTNNNNNAGG 1 cut(s) 2130
BstFNI CGCG 2 cut(s) 1060, 1062
BstH2I RGCGCY 1 cut(s) 3389
BstHHI GCGC 5 cut(s) 1062, 2400, 3388, 4871, 5193
BstMCI CGRYCG 1 cut(s) 3755
BstNI CCWGG 6 cut(s) 304, 476, 882, 1361, 1721, 3868
BstNSI RCATGY 3 cut(s) 2546, 4666, 5051
BstPAI GACNNNNGTC 1 cut(s) 2310
BstPI GGTNACC 1 cut(s) 2662
BstSCI CCNGG 7 cut(s) 302, 474, 648, 880, 1359, 1719, 3866
BstSFI CTRYAG 6 cut(s) 126, 519, 1683, 3099, 3443, 4254
BstSLI GKGCMC 3 cut(s) 561, 1281, 4652
BstUI CGCG 2 cut(s) 1060, 1062
BstV2I GAAGAC 9 cut(s) 189, 2468, 2813, 3027, 3345, 3821, 3849, 4566, 4597
BstX2I RGATCY 6 cut(s) 2639, 2837, 3089, 3367, 3976, 4629
BstXI CCANNNNNNTGG 4 cut(s) 482, 1075, 3382, 5005
BstYI RGATCY 6 cut(s) 2639, 2837, 3089, 3367, 3976, 4629
BstZ17I GTATAC 1 cut(s) 1641
Bsu15I ATCGAT 1 cut(s) 4802
Bsu36I CCTNAGG 1 cut(s) 3981
BsuRI GGCC 6 cut(s) 336, 1664, 1719, 2147, 2366, 4319
BsuTUI ATCGAT 1 cut(s) 4802
BtrI CACGTC 2 cut(s) 1328, 3571
BtsI GCAGTG 2 cut(s) 627, 3452
BveI ACCTGC 4 cut(s) 1207, 3795, 4444, 5249
Cac8I GCNNGC 4 cut(s) 1516, 2364, 3103, 4664
CaiI CAGNNNCTG 1 cut(s) 3451
CciI TCATGA 2 cut(s) 2344, 4174
CfoI GCGC 5 cut(s) 1062, 2400, 3388, 4871, 5193
Cfr10I RCCGGY 1 cut(s) 262
Cfr13I GGNCC 8 cut(s) 1357, 1717, 2122, 2145, 2569, 4317, 4754, 5219
ClaI ATCGAT 1 cut(s) 4802
CseI GACGC 1 cut(s) 2818
CsiI ACCWGGT 2 cut(s) 474, 1359
DraI TTTAAA 1 cut(s) 3340
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 8 cut(s) 33, 350, 955, 1415, 1635, 3219, 4002, 4398
EarI CTCTTC 8 cut(s) 33, 350, 955, 1415, 1635, 3219, 4002, 4398
Ecl136II GAGCTC 3 cut(s) 166, 1875, 3650
Eco130I CCWWGG 1 cut(s) 3131
Eco147I AGGCCT 1 cut(s) 2366
Eco24I GRGCYC 3 cut(s) 168, 1877, 3652
Eco31I GGTCTC 2 cut(s) 1613, 4400
Eco32I GATATC 1 cut(s) 446
Eco47I GGWCC 5 cut(s) 1357, 2122, 2569, 4754, 5219
Eco53kI GAGCTC 3 cut(s) 166, 1875, 3650
Eco57I CTGAAG 4 cut(s) 201, 2825, 3806, 4919
Eco81I CCTNAGG 1 cut(s) 3981
Eco88I CYCGRG 1 cut(s) 2353
Eco91I GGTNACC 1 cut(s) 2662
EcoICRI GAGCTC 3 cut(s) 166, 1875, 3650
EcoNI CCTNNNNNAGG 1 cut(s) 2130
EcoO109I RGGNCCY 2 cut(s) 1357, 5219
EcoO65I GGTNACC 1 cut(s) 2662
EcoRI GAATTC 3 cut(s) 1098, 2407, 4979
EcoRII CCWGG 6 cut(s) 302, 474, 880, 1359, 1719, 3866
EcoRV GATATC 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 3131
EcoT22I ATGCAT 5 cut(s) 1135, 3286, 3878, 5053, 5173
EcoT38I GRGCYC 3 cut(s) 168, 1877, 3652
ErhI CCWWGG 1 cut(s) 3131
Esp3I CGTCTC 2 cut(s) 352, 975
FauI CCCGC 1 cut(s) 5044
FauNDI CATATG 3 cut(s) 1489, 1930, 2974
FbaI TGATCA 1 cut(s) 4470
FblI GTMKAC 3 cut(s) 1557, 1640, 3670
FriOI GRGCYC 3 cut(s) 168, 1877, 3652
FspBI CTAG 9 cut(s) 1254, 1478, 2745, 3149, 3360, 3611, 3725, 5021, 5207
FspI TGCGCA 2 cut(s) 2399, 4870
GlaI GCGC 5 cut(s) 1061, 2399, 3387, 4870, 5192
GsaI CCCAGC 1 cut(s) 15
GsuI CTGGAG 6 cut(s) 239, 276, 1742, 1929, 2570, 3114
HaeII RGCGCY 1 cut(s) 3389
HaeIII GGCC 6 cut(s) 336, 1664, 1719, 2147, 2366, 4319
HapII CCGG 2 cut(s) 263, 650
HgaI GACGC 1 cut(s) 2818
HhaI GCGC 5 cut(s) 1062, 2400, 3388, 4871, 5193
Hin1I GRCGYC 1 cut(s) 361
Hin6I GCGC 5 cut(s) 1060, 2398, 3386, 4869, 5191
HinP1I GCGC 5 cut(s) 1060, 2398, 3386, 4869, 5191
HincII GTYRAC 1 cut(s) 4381
HindII GTYRAC 1 cut(s) 4381
HpaII CCGG 2 cut(s) 263, 650
HphI GGTGA 7 cut(s) 95, 106, 934, 1310, 1811, 2656, 3622
Hpy99I CGWCG 2 cut(s) 1329, 1848
HpyCH4IV ACGT 9 cut(s) 361, 584, 694, 968, 1327, 1846, 3012, 3394, 3570
HpySE526I ACGT 9 cut(s) 361, 584, 694, 968, 1327, 1846, 3012, 3394, 3570
Hsp92I GRCGYC 1 cut(s) 361
HspAI GCGC 5 cut(s) 1060, 2398, 3386, 4869, 5191
Ksp22I TGATCA 1 cut(s) 4470
MabI ACCWGGT 2 cut(s) 474, 1359
MaeI CTAG 9 cut(s) 1254, 1478, 2745, 3149, 3360, 3611, 3725, 5021, 5207
MaeII ACGT 9 cut(s) 361, 584, 694, 968, 1327, 1846, 3012, 3394, 3570
MfeI CAATTG 4 cut(s) 1888, 2073, 3533, 3950
MflI RGATCY 6 cut(s) 2639, 2837, 3089, 3367, 3976, 4629
MhlI GDGCHC 9 cut(s) 168, 262, 561, 631, 1281, 1877, 2991, 3652, 4652
MlyI GAGTC 7 cut(s) 208, 311, 1250, 1598, 1666, 3487, 4087
MmeI TCCRAC 2 cut(s) 618, 3870
Mph1103I ATGCAT 5 cut(s) 1135, 3286, 3878, 5053, 5173
MroXI GAANNNNTTC 3 cut(s) 2465, 3174, 3876
MslI CAYNNNNRTG 1 cut(s) 3380
MspA1I CMGCKG 1 cut(s) 5042
MspCI CTTAAG 1 cut(s) 1634
MspI CCGG 2 cut(s) 263, 650
MspR9I CCNGG 7 cut(s) 304, 476, 650, 882, 1361, 1721, 3868
MunI CAATTG 4 cut(s) 1888, 2073, 3533, 3950
Mva1269I GAATGC 8 cut(s) 181, 1135, 2915, 3286, 3849, 3876, 3878, 4487
MvaI CCWGG 6 cut(s) 304, 476, 882, 1361, 1721, 3868
MvnI CGCG 2 cut(s) 1060, 1062
NciI CCSGG 1 cut(s) 650
NdeI CATATG 3 cut(s) 1489, 1930, 2974
NlaIV GGNNCC 5 cut(s) 1358, 2124, 3625, 4542, 5221
NmeAIII GCCGAG 1 cut(s) 3457
NmuCI GTSAC 6 cut(s) 112, 1009, 2662, 2968, 4017, 4339
NsbI TGCGCA 2 cut(s) 2399, 4870
NsiI ATGCAT 5 cut(s) 1135, 3286, 3878, 5053, 5173
NspI RCATGY 3 cut(s) 2546, 4666, 5051
PaeI GCATGC 1 cut(s) 4666
PaeR7I CTCGAG 1 cut(s) 2353
PagI TCATGA 2 cut(s) 2344, 4174
PaqCI CACCTGC 1 cut(s) 3795
PceI AGGCCT 1 cut(s) 2366
PctI GAATGC 8 cut(s) 181, 1135, 2915, 3286, 3849, 3876, 3878, 4487
PdmI GAANNNNTTC 3 cut(s) 2465, 3174, 3876
PflMI CCANNNNNTGG 1 cut(s) 2998
Ple19I CGATCG 1 cut(s) 3755
PleI GAGTC 7 cut(s) 208, 311, 1249, 1598, 1665, 3487, 4086
PpsI GAGTC 7 cut(s) 208, 311, 1249, 1598, 1665, 3487, 4086
Ppu21I YACGTR 1 cut(s) 695
PpuMI RGGWCCY 2 cut(s) 1357, 5219
PshAI GACNNNNGTC 1 cut(s) 2310
PshBI ATTAAT 1 cut(s) 536
PsiI TTATAA 2 cut(s) 2478, 3831
Psp124BI GAGCTC 3 cut(s) 168, 1877, 3652
Psp5II RGGWCCY 2 cut(s) 1357, 5219
Psp6I CCWGG 6 cut(s) 302, 474, 880, 1359, 1719, 3866
PspEI GGTNACC 1 cut(s) 2662
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 6 cut(s) 302, 474, 880, 1359, 1719, 3866
PspN4I GGNNCC 5 cut(s) 1358, 2124, 3625, 4542, 5221
PspPI GGNCC 8 cut(s) 1357, 1717, 2122, 2145, 2569, 4317, 4754, 5219
PspPPI RGGWCCY 2 cut(s) 1357, 5219
PsrI GAACNNNNNNTAC 2 cut(s) 525, 557
PstI CTGCAG 4 cut(s) 130, 523, 3103, 3447
PstNI CAGNNNCTG 1 cut(s) 3451
PsuI RGATCY 6 cut(s) 2639, 2837, 3089, 3367, 3976, 4629
PvuI CGATCG 1 cut(s) 3755
PvuII CAGCTG 1 cut(s) 5042
RseI CAYNNNNRTG 1 cut(s) 3380
SacI GAGCTC 3 cut(s) 168, 1877, 3652
Sau96I GGNCC 8 cut(s) 1357, 1717, 2122, 2145, 2569, 4317, 4754, 5219
ScaI AGTACT 1 cut(s) 3211
SchI GAGTC 7 cut(s) 208, 311, 1250, 1598, 1666, 3487, 4087
ScrFI CCNGG 7 cut(s) 304, 476, 650, 882, 1361, 1721, 3868
SduI GDGCHC 9 cut(s) 168, 262, 561, 631, 1281, 1877, 2991, 3652, 4652
SexAI ACCWGGT 2 cut(s) 474, 1359
SfcI CTRYAG 6 cut(s) 126, 519, 1683, 3099, 3443, 4254
Sfr274I CTCGAG 1 cut(s) 2353
SinI GGWCC 5 cut(s) 1357, 2122, 2569, 4754, 5219
SlaI CTCGAG 1 cut(s) 2353
SmiMI CAYNNNNRTG 1 cut(s) 3380
SmlI CTYRAG 4 cut(s) 1634, 1697, 2353, 4787
SmoI CTYRAG 4 cut(s) 1634, 1697, 2353, 4787
SphI GCATGC 1 cut(s) 4666
SseBI AGGCCT 1 cut(s) 2366
SsiI CCGC 6 cut(s) 682, 1379, 2632, 2981, 4930, 5037
SspI AATATT 1 cut(s) 2240
SspMI CTAG 9 cut(s) 1254, 1478, 2745, 3149, 3360, 3611, 3725, 5021, 5207
SstI GAGCTC 3 cut(s) 168, 1877, 3652
StuI AGGCCT 1 cut(s) 2366
StyD4I CCNGG 7 cut(s) 302, 474, 648, 880, 1359, 1719, 3866
StyI CCWWGG 1 cut(s) 3131
TaiI ACGT 9 cut(s) 364, 587, 697, 971, 1330, 1849, 3015, 3397, 3573
TaqI TCGA 6 cut(s) 1324, 1705, 2354, 4526, 4692, 4802
TaqII GACCGA 1 cut(s) 248
TatI WGTACW 7 cut(s) 192, 1234, 1670, 3209, 3573, 4168, 4874
TauI GCSGC 3 cut(s) 685, 2635, 4933
TseFI GTSAC 6 cut(s) 112, 1009, 2662, 2968, 4017, 4339
Tsp45I GTSAC 6 cut(s) 112, 1009, 2662, 2968, 4017, 4339
TspGWI ACGGA 3 cut(s) 1351, 2309, 2850
Van91I CCANNNNNTGG 1 cut(s) 2998
Vha464I CTTAAG 1 cut(s) 1634
VneI GTGCAC 1 cut(s) 4648
VpaK11BI GGWCC 5 cut(s) 1357, 2122, 2569, 4754, 5219
VspI ATTAAT 1 cut(s) 536
XagI CCTNNNNNAGG 1 cut(s) 2130
XapI RAATTY 8 cut(s) 455, 1098, 1349, 2407, 2576, 2618, 4602, 4979
XbaI TCTAGA 1 cut(s) 2744
XceI RCATGY 3 cut(s) 2546, 4666, 5051
XcmI CCANNNNNNNNNTGG 1 cut(s) 4735
XhoI CTCGAG 1 cut(s) 2353
XmiI GTMKAC 3 cut(s) 1557, 1640, 3670
XmnI GAANNNNTTC 3 cut(s) 2465, 3174, 3876
XspI CTAG 9 cut(s) 1254, 1478, 2745, 3149, 3360, 3611, 3725, 5021, 5207
ZraI GACGTC 1 cut(s) 362
ZrmI AGTACT 1 cut(s) 3211
Zsp2I ATGCAT 5 cut(s) 1135, 3286, 3878, 5053, 5173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.