Rroxscaffold_6G00408830

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003946
Physical Location & Seq
Reverse (-)
31306984 .. 31311962
4979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408830.1

Sequence Viewer

Length: 3696 bp
ATGACCAAAGAAGAAGACGCTGCTAAACATCACATAGAAGAGATCAGGACCCAAAAGTTCTCCATAGGGAAGAAAGACTCAAATCCCTTAACTCTTGATCTGCACCATGCAGTCACTAGTCTATCTGCCGAGCTTTATCAGAAAGACATCCATTTCCTAATGGAGCTCATACAGAATGCAGAGGACAATGAGGACAAAGAAGGTGTAGAGCCAACGCTGGAATTTGTGCTGACCAAGAAAGACATAACGGGGTCTGGAGCATCTGCTACTCTGCTCGTTTTCAACAATGAGGTTGGCTTTACTAGGAAGAACATCGATTCCATATGCAGCATAGGCCGTTCTACCAAGAAGGGGAAGAGACAACAAGGCTTTATTGGAGAAAAAGGAATTGGATTCAAAAGTGTGTTTCTTGTGTCTTCACAACCGCACATATTTAGCAATGGATATCGTGTCAGATTTGATGAAGAACCAGACCAGGATTGTGGTATTGGGTATGTAGCTCCTCAATGGGTTTCCGGAAAGCCTTACTTGTCAAGCATATGTGATGTGTATGGGTCTGAGAAGATCTTGCCAACGACTACATTCATCCTTCCTCTAAAGCTTGACAAGGTGGAAGCGGTAAGAGCACAATTGTCAGAGCTGCACCCAGAGATTCTCTTGTTTTTGTCCAAGGTAAAGCGGCTATATGTACACGGGTGTGATCCTGAAGAAGCTGATGATGTCTCCACAATATCTATATTTAGTGAGACTGAACATCTGAAGTTGAGCGATAAAACAGCCAAGTCACGATTTGTTCAACTTTCAGTCAAAGAAAAGATGTGTGATGCTGAGCAGCTGTGCAAGTATTTCTTATGGAGAGAGGAATTTCCCGTAAACCCTGGAAATAAAGTTCGTATAAGGATGGATGTGGAGAAATGGGTGATTACCCTTGCTTTCCCATTTGGAGAAAGATTGAGAAGGGGAACATCATCTGTTGGTATATTTGCTTTCCTGCCCACAGCGATGGTTATAAACCTTCCGTTTATAATTCAAGCAGATTTCATCCTTGCTTCTTCACGAGAATCTATAATGTTGGACAATGTATGGAATTTGGGAATTCTTAAATGTGCCCCTTCAGCTTTTGTGAATGCTTTTCAGTCTTGTGTGGAAGACTTCTCTCTGTTTCCTTCCATAAGTCAGCCATTTGAGTTTTTACCTGCTCAAGATTCACCAATTCCTGAGTTTAGCAACCTAAGAGAGTCTATTAGAACTAACTTGCAAGATTTAAAGATAGTGCCCTGTCAAGTATTTCCTGGTAATCGTTTATTCCTGCAGCCTCAATATGCACACCGGATCCTGCCAAAATTTAGGGACCTACTGGTTCGAATTAGAAGTGAAGGGGCTGTTTCAAGTGGTTCAGCTTCATTGAAGAGAGTCTTGCATCCATATCTGGATATTGAAAAATACAGTGCAGTTCTGGAGTTCCTAGGTGTGACATCTTCCAGTGGTGCTTGGTACACAAACTGCATCAACTCCTGCAATCTTGTTTTGTTATCTGATGAGGTCTATATTCAACTACTTGGTTTCATTGCTGATAATAAGGAAAAATTACTTCGAACTAGGGGTTCATGTTTACCTGCAAAGTTCATTGAAAGCATTCGAGTTGGAAAATGGATGAAGACATATACAGGCTATGTTTCTCCAAGACTGGCTTTTCTTCCCAATGAAACAGGGAAAATTATTTTTGATATGATGAAGCATGTTATGGAGGATATCTCCATTATAGACGTTGAGTTTTATGAGAATCAAATCACTCTTTATCAAGATGAATTGAAATTTCTTGATGTGGGGCTTGGATCAGAAGCTGTGAGGAAGCTGGTTTCTAATCACTTTAAGTCTTTTACTTCCCAGGGAATGAGCAAAGAGTTCAGCTTTGCTTTGCTGGATTTCATTAGTTTCTCTAAACGAAGAATGATGGTGGATAAGGATTGGTTGTCTGTTATGAAGCAGAAGAAGTGGCTGAAGACTCATCATGGCTATGATGCTCCTAAGGGATCTATTCTCTTGCCATCTGACCTAGAAGCTGAAACATGTTCAAAAGTTACAAATCTTCCTATTGTTGATGAAGCATTCTATGGTAGCGGATTAAGATCTTTCTTATCTGATTTAAGGCTACTTGAGGTTGAATATGACAGGGAGGAGGTGCAAAAATTAATTGCAGAGAATGTAATATTGACTCCAAATCTGTCTTCGATGACTGGTAGTTGTGGTTTATTGCTTCTTAAATGTATCATATGTTTGGGAAGTGGAGCTGCTGGTGTGATTAATATGATTAAATGTCAACCTTGGATGAAGACAACTTTTGGTTTTAGATGCCCTGAAGAAACTGTCCTTATTGATCCTTGTTGGGGTTCTTTGTTGAGTGTTCTTCAGGTGCCTACCATAGATGAATTGTACTATGGAAATGAAATGAGGCATTTCATTGATGAGTTGCAAGCCATCAGAGTGGTGGTTGGAAATGTTGGAGTAAGTAATACGATTGCTGCTCGGTTTAAATCAATCTTGTCTTCTTCTGGTCTGGCGCCTTCCAATGTCCTGTCTCTATTAGGTTGCATTGGAGAAATCAGTCAAACGATGTCTTTACAGTCCCCTGAATTGAATTGGTTGCTATGTGAGAAATGGCTGAAAACACGTCATGGTTATAAGACTCCTAATGAATCAATTATTTTCAGCTCTAAATGGGGATCAACCTTGTTGTATGTGGATCTCCCTCTCGTAGATGATATTTATTATGGTCTTGGCATATATAAGTTCAAGGATGAACTCCAGATGCTGGGTGCCATCACGGATTTTGAAGGAGGAGCTCTTTTTGTAGCTAAAGGTCTATGCAGCCCAATTGACCCTGAGCTAGTTTCTGCAGATGGATTTATATCATTGCTAGAGTGCCTCAAATCCCTGAAGTCGAGGTCTCTTGATGACCCCTTGCTGGGTGACTTTGTCAAGAACATTGCAAAAAGCAGGTGTCTAAAGATCAGGAATGGGTACAAAACGCCTGAAGAATGTGTTCTGTTTGATCCAGCCTGGGAAGGTATTCTGAAGCGATCAGATGCACCAACAATTGATGAAAGTTTTACTGGAACTGACATTTCTTTATACAAGAATCAGTTGAGAGATATTGGTGTGAAGGTAGATCCTCTCGAGGTTTGTTCTCTTCTTTCTAGGCTTCTTTTGTCACTGACAGACACGGCATATATAACAAGGATTTATAGCTTCCTTACTGAGTTCTTCTGGAGTCCCCAAGTGCTAGATAATTGCAACTCTAAGGTTTGGATACCTGACCCAAAGGGTACTGGTGAATGGGTTAATTCTCTGGATTGCATACTTCATGATAGGAAAAATCTCTTTGGTTCTCGCTTTTTTTGTCTTGACAAGTTGTATAGGAAAGAGCTTCTCCCCATGTTTTGTTCAGCCTTTGGAGTTGCAGAACATCCTTCCATCAATGATTACTTGCAGCTATGGAGCACATGGGCTATGAGGGATGACTTCCAAGTGACTGTTGAAGAGTGCAACTCGTTTTGGGGATGTGTATTCGACAACTGGAATCAACATGTTGAAGATACTCTGAAGGAGAAGTTGACCAAATTACCTGCCACGATTTTGTCCATGGTTGGGGAAATATATATATGTGATGAGCAGAGAGGAAGTATTCATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1231

Amino Acids

139.01

Weight (kDa)

5.61

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 46 - 154 5.3e-11 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1010, 1025, 2682
AarI CACCTGC 1 cut(s) 2988
Acc36I ACCTGC 4 cut(s) 1204, 1624, 2988, 3634
AccB1I GGYRCC 3 cut(s) 2413, 2560, 2816
AccB7I CCANNNNNTGG 1 cut(s) 2812
AccIII TCCGGA 1 cut(s) 515
AciI CCGC 4 cut(s) 425, 617, 679, 2121
AcsI RAATTY 6 cut(s) 221, 863, 1087, 1095, 1343, 1814
AcyI GRCGYC 1 cut(s) 2561
AfaI GTAC 5 cut(s) 690, 1496, 2435, 3023, 3328
AfiI CCNNNNNNNGG 6 cut(s) 351, 2387, 2812, 2965, 2966, 3648
AflIII ACRYGT 3 cut(s) 2069, 2669, 3586
AhlI ACTAGT 1 cut(s) 116
AjiI CACGTC 1 cut(s) 2672
AjnI CCWGG 5 cut(s) 474, 877, 1291, 1887, 3059
AjuI GAANNNNNNNTTGG 4 cut(s) 372, 404, 2212, 2244
AleI CACNNNNGTG 1 cut(s) 696
AloI GAACNNNNNNTCC 4 cut(s) 302, 334, 873, 905
Alw21I GWGCWC 4 cut(s) 168, 628, 2845, 3503
Alw26I GTCTC 5 cut(s) 352, 727, 740, 2583, 2952
AlwNI CAGNNNCTG 2 cut(s) 1844, 2812
Ama87I CYCGRG 1 cut(s) 3176
Aor13HI TCCGGA 1 cut(s) 515
AoxI GGCC 1 cut(s) 334
ApeKI GCWGC 9 cut(s) 20, 327, 640, 832, 1312, 2291, 2522, 2868, 3490
ApoI RAATTY 6 cut(s) 221, 863, 1087, 1095, 1343, 1814
AseI ATTAAT 2 cut(s) 2192, 2304
Asp700I GAANNNNTTC 3 cut(s) 1635, 3042, 3069
AspA2I CCTAGG 1 cut(s) 1465
AspLEI GCGC 1 cut(s) 2563
AspS9I GGNCC 2 cut(s) 48, 1351
AsuHPI GGTGA 4 cut(s) 931, 1200, 2981, 3344
AsuII TTCGAA 2 cut(s) 1363, 1594
AvaI CYCGRG 1 cut(s) 3176
AvaII GGWCC 2 cut(s) 48, 1351
AvrII CCTAGG 1 cut(s) 1465
AxyI CCTNAGG 1 cut(s) 2028
BaeGI GKGCMC 2 cut(s) 1111, 1278
BamHI GGATCC 1 cut(s) 1332
BanI GGYRCC 3 cut(s) 2413, 2560, 2816
BanII GRGCYC 2 cut(s) 168, 2845
BauI CACGAG 1 cut(s) 1056
BbsI GAAGAC 8 cut(s) 21, 408, 1155, 1664, 2009, 2220, 2339, 2538
Bbv12I GWGCWC 4 cut(s) 168, 628, 2845, 3503
BbvI GCAGC 9 cut(s) 7, 339, 627, 844, 1324, 2278, 2509, 2880, 3502
BccI CCATC 8 cut(s) 895, 997, 1948, 2056, 2486, 2828, 2894, 3482
BceAI ACGGC 2 cut(s) 321, 3240
BciT130I CCWGG 5 cut(s) 476, 879, 1293, 1889, 3061
BciVI GTATCC 1 cut(s) 3303
BcoDI GTCTC 5 cut(s) 352, 727, 740, 2583, 2952
BcuI ACTAGT 1 cut(s) 116
BfaI CTAG 9 cut(s) 117, 303, 1466, 1599, 2057, 2888, 2918, 3198, 3284
BfmI CTRYAG 2 cut(s) 1310, 2895
BfoI RGCGCY 1 cut(s) 2564
BfuAI ACCTGC 4 cut(s) 1204, 1624, 2988, 3634
BfuI GTATCC 1 cut(s) 3303
BglII AGATCT 2 cut(s) 564, 2129
BlnI CCTAGG 1 cut(s) 1465
BlpI GCTNAGC 1 cut(s) 828
Bme1390I CCNGG 5 cut(s) 476, 879, 1293, 1889, 3061
Bme18I GGWCC 2 cut(s) 48, 1351
BmeT110I CYCGRG 1 cut(s) 3176
BmgBI CACGTC 1 cut(s) 2672
BmgT120I GGNCC 2 cut(s) 48, 1351
BmiI GGNNCC 6 cut(s) 50, 1334, 1352, 2415, 2562, 2818
BmrFI CCNGG 5 cut(s) 476, 879, 1293, 1889, 3061
BmsI GCATC 8 cut(s) 269, 814, 1429, 1515, 2011, 2342, 2799, 3076
BpiI GAAGAC 8 cut(s) 21, 408, 1155, 1664, 2009, 2220, 2339, 2538
BplI GAGNNNNNCTC 4 cut(s) 1739, 1771, 3533, 3565
BpmI CTGGAG 4 cut(s) 276, 1478, 2789, 3289
Bpu10I CCTNAGC 1 cut(s) 2883
Bpu1102I GCTNAGC 1 cut(s) 828
Bpu14I TTCGAA 2 cut(s) 1363, 1594
BpuEI CTTGAG 2 cut(s) 1185, 2177
Bsa29I ATCGAT 1 cut(s) 315
BsaBI GATNNNNATC 2 cut(s) 2128, 2908
BsaHI GRCGYC 1 cut(s) 2561
BsaI GGTCTC 1 cut(s) 2952
BsaJI CCNNGG 8 cut(s) 669, 877, 1465, 1887, 1888, 2324, 3060, 3642
BsaWI WCCGGW 2 cut(s) 515, 1329
Bsc4I CCNNNNNNNGG 6 cut(s) 351, 2387, 2812, 2965, 2966, 3648
Bse1I ACTGG 7 cut(s) 1362, 1482, 1692, 2242, 3118, 3334, 3581
Bse21I CCTNAGG 1 cut(s) 2028
Bse3DI GCAATG 5 cut(s) 445, 1566, 2912, 2985, 3688
Bse8I GATNNNNATC 2 cut(s) 2128, 2908
BseAI TCCGGA 1 cut(s) 515
BseBI CCWGG 5 cut(s) 476, 879, 1293, 1889, 3061
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 8 cut(s) 669, 877, 1465, 1887, 1888, 2324, 3060, 3642
BseJI GATNNNNATC 2 cut(s) 2128, 2908
BseLI CCNNNNNNNGG 6 cut(s) 351, 2387, 2812, 2965, 2966, 3648
BseMI GCAATG 5 cut(s) 445, 1566, 2912, 2985, 3688
BseMII CTCAG 5 cut(s) 549, 819, 1209, 2874, 3249
BseNI ACTGG 7 cut(s) 1362, 1482, 1692, 2242, 3118, 3334, 3581
BseRI GAGGAG 3 cut(s) 492, 2192, 2853
BseSI GKGCMC 2 cut(s) 1111, 1278
BseXI GCAGC 9 cut(s) 7, 339, 627, 844, 1324, 2278, 2509, 2880, 3502
BseYI CCCAGC 2 cut(s) 2812, 2965
BsgI GTGCAG 3 cut(s) 86, 626, 1470
BshFI GGCC 1 cut(s) 336
BshNI GGYRCC 3 cut(s) 2413, 2560, 2816
BshVI ATCGAT 1 cut(s) 315
BsiHKAI GWGCWC 4 cut(s) 168, 628, 2845, 3503
BsiHKCI CYCGRG 1 cut(s) 3176
BsiSI CCGG 2 cut(s) 516, 1330
BslFI GGGAC 3 cut(s) 1364, 2611, 3258
BslI CCNNNNNNNGG 6 cut(s) 351, 2387, 2812, 2965, 2966, 3648
BsmAI GTCTC 5 cut(s) 352, 727, 740, 2583, 2952
BsmFI GGGAC 3 cut(s) 1364, 2611, 3258
BsmI GAATGC 4 cut(s) 181, 1132, 1635, 2108
BsnI GGCC 1 cut(s) 336
Bso31I GGTCTC 1 cut(s) 2952
BsoBI CYCGRG 1 cut(s) 3176
Bsp119I TTCGAA 2 cut(s) 1363, 1594
Bsp1286I GDGCHC 6 cut(s) 168, 628, 1111, 1278, 2845, 3503
Bsp13I TCCGGA 1 cut(s) 515
Bsp1407I TGTACA 1 cut(s) 688
Bsp1720I GCTNAGC 1 cut(s) 828
Bsp19I CCATGG 1 cut(s) 3642
BspACI CCGC 4 cut(s) 425, 617, 679, 2121
BspANI GGCC 1 cut(s) 336
BspCNI CTCAG 5 cut(s) 550, 820, 1210, 2875, 3250
BspDI ATCGAT 1 cut(s) 315
BspEI TCCGGA 1 cut(s) 515
BspHI TCATGA 1 cut(s) 3364
BspLI GGNNCC 6 cut(s) 50, 1334, 1352, 2415, 2562, 2818
BspMAI CTGCAG 2 cut(s) 1314, 2899
BspMI ACCTGC 4 cut(s) 1204, 1624, 2988, 3634
BspT104I TTCGAA 2 cut(s) 1363, 1594
BspT107I GGYRCC 3 cut(s) 2413, 2560, 2816
BspTNI GGTCTC 1 cut(s) 2952
BsrDI GCAATG 5 cut(s) 445, 1566, 2912, 2985, 3688
BsrGI TGTACA 1 cut(s) 688
BsrI ACTGG 7 cut(s) 1362, 1482, 1692, 2242, 3118, 3334, 3581
BssECI CCNNGG 8 cut(s) 669, 877, 1465, 1887, 1888, 2324, 3060, 3642
BssNI GRCGYC 1 cut(s) 2561
BssSI CACGAG 1 cut(s) 1056
BssT1I CCWWGG 4 cut(s) 669, 1465, 2324, 3642
Bst2BI CACGAG 1 cut(s) 1056
Bst2UI CCWGG 5 cut(s) 476, 879, 1293, 1889, 3061
Bst4CI ACNGT 4 cut(s) 1448, 2368, 2625, 3535
Bst6I CTCTTC 5 cut(s) 33, 350, 1403, 3195, 3534
BstACI GRCGYC 1 cut(s) 2561
BstAUI TGTACA 1 cut(s) 688
BstBI TTCGAA 2 cut(s) 1363, 1594
BstC8I GCNNGC 1 cut(s) 2475
BstDEI CTNAG 8 cut(s) 558, 828, 1218, 1232, 2028, 2883, 3258, 3300
BstDSI CCRYGG 1 cut(s) 3642
BstH2I RGCGCY 1 cut(s) 2564
BstHHI GCGC 1 cut(s) 2563
BstMAI GTCTC 5 cut(s) 352, 727, 740, 2583, 2952
BstMWI GCNNNNNNNGC 3 cut(s) 333, 623, 1115
BstNI CCWGG 5 cut(s) 476, 879, 1293, 1889, 3061
BstNSI RCATGY 3 cut(s) 1742, 2073, 3590
BstSCI CCNGG 5 cut(s) 474, 877, 1291, 1887, 3059
BstSFI CTRYAG 2 cut(s) 1310, 2895
BstSLI GKGCMC 2 cut(s) 1111, 1278
BstV1I GCAGC 9 cut(s) 7, 339, 627, 844, 1324, 2278, 2509, 2880, 3502
BstV2I GAAGAC 8 cut(s) 21, 408, 1155, 1664, 2009, 2220, 2339, 2538
BstX2I RGATCY 6 cut(s) 564, 1332, 2033, 2129, 2743, 3169
BstXI CCANNNNNNTGG 3 cut(s) 482, 1003, 2485
BstYI RGATCY 6 cut(s) 564, 1332, 2033, 2129, 2743, 3169
Bsu15I ATCGAT 1 cut(s) 315
Bsu36I CCTNAGG 1 cut(s) 2028
BsuI GTATCC 1 cut(s) 3303
BsuRI GGCC 1 cut(s) 336
BsuTUI ATCGAT 1 cut(s) 315
BtgI CCRYGG 1 cut(s) 3642
BtgZI GCGATG 1 cut(s) 1016
BtrI CACGTC 1 cut(s) 2672
BtsIMutI CAGTG 3 cut(s) 1453, 1489, 3212
BveI ACCTGC 4 cut(s) 1204, 1624, 2988, 3634
Cac8I GCNNGC 1 cut(s) 2475
CaiI CAGNNNCTG 2 cut(s) 1844, 2812
CciI TCATGA 1 cut(s) 3364
CfoI GCGC 1 cut(s) 2563
Cfr13I GGNCC 2 cut(s) 48, 1351
ClaI ATCGAT 1 cut(s) 315
CseI GACGC 1 cut(s) 26
Csp6I GTAC 5 cut(s) 689, 1495, 2434, 3022, 3327
CviQI GTAC 5 cut(s) 689, 1495, 2434, 3022, 3327
DdeI CTNAG 8 cut(s) 558, 828, 1218, 1232, 2028, 2883, 3258, 3300
DinI GGCGCC 1 cut(s) 2562
DraI TTTAAA 2 cut(s) 1266, 2533
Eam1104I CTCTTC 5 cut(s) 33, 350, 1403, 3195, 3534
EarI CTCTTC 5 cut(s) 33, 350, 1403, 3195, 3534
Ecl136II GAGCTC 2 cut(s) 166, 2843
Eco130I CCWWGG 4 cut(s) 669, 1465, 2324, 3642
Eco24I GRGCYC 2 cut(s) 168, 2845
Eco31I GGTCTC 1 cut(s) 2952
Eco32I GATATC 2 cut(s) 446, 1753
Eco47I GGWCC 2 cut(s) 48, 1351
Eco53kI GAGCTC 2 cut(s) 166, 2843
Eco81I CCTNAGG 1 cut(s) 2028
Eco88I CYCGRG 1 cut(s) 3176
EcoICRI GAGCTC 2 cut(s) 166, 2843
EcoO109I RGGNCCY 2 cut(s) 48, 1351
EcoRI GAATTC 1 cut(s) 1095
EcoRII CCWGG 5 cut(s) 474, 877, 1291, 1887, 3059
EcoRV GATATC 2 cut(s) 446, 1753
EcoT14I CCWWGG 4 cut(s) 669, 1465, 2324, 3642
EcoT38I GRGCYC 2 cut(s) 168, 2845
EgeI GGCGCC 1 cut(s) 2562
EheI GGCGCC 1 cut(s) 2562
ErhI CCWWGG 4 cut(s) 669, 1465, 2324, 3642
FalI AAGNNNNNCTT 8 cut(s) 512, 544, 833, 865, 1400, 1432, 1675, 1707
FaqI GGGAC 3 cut(s) 1364, 2611, 3258
FauNDI CATATG 3 cut(s) 323, 539, 2273
FriOI GRGCYC 2 cut(s) 168, 2845
FspBI CTAG 9 cut(s) 117, 303, 1466, 1599, 2057, 2888, 2918, 3198, 3284
GlaI GCGC 1 cut(s) 2562
GsaI CCCAGC 2 cut(s) 2816, 2969
GsuI CTGGAG 4 cut(s) 276, 1478, 2789, 3289
HaeII RGCGCY 1 cut(s) 2564
HaeIII GGCC 1 cut(s) 336
HapII CCGG 2 cut(s) 516, 1330
HgaI GACGC 1 cut(s) 26
HhaI GCGC 1 cut(s) 2563
Hin1I GRCGYC 1 cut(s) 2561
Hin6I GCGC 1 cut(s) 2561
HinP1I GCGC 1 cut(s) 2561
HincII GTYRAC 2 cut(s) 2321, 3615
HindII GTYRAC 2 cut(s) 2321, 3615
HindIII AAGCTT 1 cut(s) 599
HpaII CCGG 2 cut(s) 516, 1330
HphI GGTGA 4 cut(s) 931, 1200, 2981, 3344
Hpy166II GTNNAC 6 cut(s) 691, 874, 1497, 1613, 2321, 3615
Hpy8I GTNNAC 6 cut(s) 691, 874, 1497, 1613, 2321, 3615
HpyCH4III ACNGT 4 cut(s) 1448, 2368, 2625, 3535
HpyCH4IV ACGT 2 cut(s) 1767, 2671
HpyF10VI GCNNNNNNNGC 3 cut(s) 333, 623, 1115
HpyF3I CTNAG 8 cut(s) 558, 828, 1218, 1232, 2028, 2883, 3258, 3300
HpySE526I ACGT 2 cut(s) 1767, 2671
Hsp92I GRCGYC 1 cut(s) 2561
HspAI GCGC 1 cut(s) 2561
KasI GGCGCC 1 cut(s) 2560
Kpn2I TCCGGA 1 cut(s) 515
LmnI GCTCC 7 cut(s) 163, 257, 505, 2029, 2288, 2840, 3498
Lsp1109I GCAGC 9 cut(s) 7, 339, 627, 844, 1324, 2278, 2509, 2880, 3502
LweI GCATC 8 cut(s) 269, 814, 1429, 1515, 2011, 2342, 2799, 3076
MaeI CTAG 9 cut(s) 117, 303, 1466, 1599, 2057, 2888, 2918, 3198, 3284
MaeII ACGT 2 cut(s) 1767, 2671
MaeIII GTNAC 7 cut(s) 112, 783, 1471, 2080, 2969, 3210, 3529
MfeI CAATTG 3 cut(s) 629, 2874, 3096
MflI RGATCY 6 cut(s) 564, 1332, 2033, 2129, 2743, 3169
MhlI GDGCHC 6 cut(s) 168, 628, 1111, 1278, 2845, 3503
Mly113I GGCGCC 1 cut(s) 2561
MlyI GAGTC 7 cut(s) 71, 1247, 1422, 1999, 2209, 2680, 3280
MmeI TCCRAC 4 cut(s) 1053, 1624, 2473, 2482
MroI TCCGGA 1 cut(s) 515
MroXI GAANNNNTTC 3 cut(s) 1635, 3042, 3069
MslI CAYNNNNRTG 4 cut(s) 696, 1001, 2016, 2483
MspA1I CMGCKG 1 cut(s) 835
MspI CCGG 2 cut(s) 516, 1330
MspR9I CCNGG 5 cut(s) 476, 879, 1293, 1889, 3061
MunI CAATTG 3 cut(s) 629, 2874, 3096
Mva1269I GAATGC 4 cut(s) 181, 1132, 1635, 2108
MvaI CCWGG 5 cut(s) 476, 879, 1293, 1889, 3061
MwoI GCNNNNNNNGC 3 cut(s) 333, 623, 1115
NarI GGCGCC 1 cut(s) 2561
NcoI CCATGG 1 cut(s) 3642
NdeI CATATG 3 cut(s) 323, 539, 2273
NlaIV GGNNCC 6 cut(s) 50, 1334, 1352, 2415, 2562, 2818
NmeAIII GCCGAG 1 cut(s) 154
NmuCI GTSAC 6 cut(s) 112, 783, 1471, 2969, 3210, 3529
NspI RCATGY 3 cut(s) 1742, 2073, 3590
NspV TTCGAA 2 cut(s) 1363, 1594
OliI CACNNNNGTG 1 cut(s) 696
PaeR7I CTCGAG 1 cut(s) 3176
PagI TCATGA 1 cut(s) 3364
PaqCI CACCTGC 1 cut(s) 2988
PasI CCCWGGG 1 cut(s) 1888
PciI ACATGT 2 cut(s) 2069, 3586
PctI GAATGC 4 cut(s) 181, 1132, 1635, 2108
PdmI GAANNNNTTC 3 cut(s) 1635, 3042, 3069
PfeI GAWTC 9 cut(s) 317, 393, 652, 1061, 1205, 1783, 2696, 3139, 3580
PflFI GACNNNGTC 1 cut(s) 2975
PflMI CCANNNNNTGG 1 cut(s) 2812
PleI GAGTC 7 cut(s) 71, 1246, 1421, 1999, 2209, 2680, 3279
PluTI GGCGCC 1 cut(s) 2564
PpsI GAGTC 7 cut(s) 71, 1246, 1421, 1999, 2209, 2680, 3279
PpuMI RGGWCCY 2 cut(s) 48, 1351
PscI ACATGT 2 cut(s) 2069, 3586
PshBI ATTAAT 2 cut(s) 2192, 2304
PsiI TTATAA 3 cut(s) 1010, 1025, 2682
Psp124BI GAGCTC 2 cut(s) 168, 2845
Psp5II RGGWCCY 2 cut(s) 48, 1351
Psp6I CCWGG 5 cut(s) 474, 877, 1291, 1887, 3059
PspFI CCCAGC 2 cut(s) 2812, 2965
PspGI CCWGG 5 cut(s) 474, 877, 1291, 1887, 3059
PspN4I GGNNCC 6 cut(s) 50, 1334, 1352, 2415, 2562, 2818
PspPI GGNCC 2 cut(s) 48, 1351
PspPPI RGGWCCY 2 cut(s) 48, 1351
PstI CTGCAG 2 cut(s) 1314, 2899
PstNI CAGNNNCTG 2 cut(s) 1844, 2812
PsuI RGATCY 6 cut(s) 564, 1332, 2033, 2129, 2743, 3169
PsyI GACNNNGTC 1 cut(s) 2975
PvuII CAGCTG 1 cut(s) 835
RsaI GTAC 5 cut(s) 690, 1496, 2435, 3023, 3328
RsaNI GTAC 5 cut(s) 689, 1495, 2434, 3022, 3327
RseI CAYNNNNRTG 4 cut(s) 696, 1001, 2016, 2483
SacI GAGCTC 2 cut(s) 168, 2845
Sau96I GGNCC 2 cut(s) 48, 1351
SchI GAGTC 7 cut(s) 71, 1247, 1422, 1999, 2209, 2680, 3280
ScrFI CCNGG 5 cut(s) 476, 879, 1293, 1889, 3061
SduI GDGCHC 6 cut(s) 168, 628, 1111, 1278, 2845, 3503
SfaNI GCATC 8 cut(s) 269, 814, 1429, 1515, 2011, 2342, 2799, 3076
SfcI CTRYAG 2 cut(s) 1310, 2895
SfoI GGCGCC 1 cut(s) 2562
Sfr274I CTCGAG 1 cut(s) 3176
SfuI TTCGAA 2 cut(s) 1363, 1594
SinI GGWCC 2 cut(s) 48, 1351
SlaI CTCGAG 1 cut(s) 3176
SmiMI CAYNNNNRTG 4 cut(s) 696, 1001, 2016, 2483
SmlI CTYRAG 3 cut(s) 1200, 2156, 3176
SmoI CTYRAG 3 cut(s) 1200, 2156, 3176
SpeI ACTAGT 1 cut(s) 116
SsiI CCGC 4 cut(s) 425, 617, 679, 2121
SspDI GGCGCC 1 cut(s) 2560
SspI AATATT 1 cut(s) 2211
SspMI CTAG 9 cut(s) 117, 303, 1466, 1599, 2057, 2888, 2918, 3198, 3284
SstI GAGCTC 2 cut(s) 168, 2845
StyD4I CCNGG 5 cut(s) 474, 877, 1291, 1887, 3059
StyI CCWWGG 4 cut(s) 669, 1465, 2324, 3642
TaaI ACNGT 4 cut(s) 1448, 2368, 2625, 3535
TaiI ACGT 2 cut(s) 1770, 2674
TaqI TCGA 8 cut(s) 315, 1363, 1594, 1639, 2231, 2942, 3177, 3570
TatI WGTACW 2 cut(s) 688, 2433
TauI GCSGC 1 cut(s) 682
TfiI GAWTC 9 cut(s) 317, 393, 652, 1061, 1205, 1783, 2696, 3139, 3580
TscAI CASTG 3 cut(s) 1453, 1489, 3219
TseFI GTSAC 6 cut(s) 112, 783, 1471, 2969, 3210, 3529
TseI GCWGC 9 cut(s) 20, 327, 640, 832, 1312, 2291, 2522, 2868, 3490
Tsp45I GTSAC 6 cut(s) 112, 783, 1471, 2969, 3210, 3529
TspGWI ACGGA 2 cut(s) 1008, 2840
TspRI CASTG 3 cut(s) 1453, 1489, 3219
Tth111I GACNNNGTC 1 cut(s) 2975
Van91I CCANNNNNTGG 1 cut(s) 2812
VpaK11BI GGWCC 2 cut(s) 48, 1351
VspI ATTAAT 2 cut(s) 2192, 2304
XapI RAATTY 6 cut(s) 221, 863, 1087, 1095, 1343, 1814
XceI RCATGY 3 cut(s) 1742, 2073, 3590
XcmI CCANNNNNNNNNTGG 1 cut(s) 2485
XhoI CTCGAG 1 cut(s) 3176
XmaJI CCTAGG 1 cut(s) 1465
XmnI GAANNNNTTC 3 cut(s) 1635, 3042, 3069
XspI CTAG 9 cut(s) 117, 303, 1466, 1599, 2057, 2888, 2918, 3198, 3284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.