RLG00000024070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003946
Physical Location & Seq
Reverse (-)
31460140 .. 31465942
5803 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024070

Sequence Viewer

Length: 5301 bp
ATGGAAAGCTCCTCAACTGAAGCTTTTATAAGCTCTGTAATGACCAAAGAAGAAGACGCTGCTAAACTTCACATAGAAGAGATCAGGACCCAGAAGTTCTCCTTAGCGAAGAAAGACTCAAATCCCTTAACGCTTGATCTACACCATGCAGTCACTAGTCTATCTGGCGAGCTTTATCAGAAAGACATCCATTTCCTAATGGAGCTCATACAGAATGCGGAGGACAATGAGTACAAAGAAGGTGTAGAGCCAACGCTGGAATTTGTGCTGACCAAGAAAGACATAACCGGGTCTGGAGCATCTGCTACTCTGCTCGTTTTCAACAATGAGGTTGGCTTTACTAGGAAGAACATCGATTCCATATGCAGCATAGGCCGTTCTACCAAGAAGGGGAAGAGACAACAGGGCTTTATTGGAGAAAAAGGAATTGGATTCAAAAGTGTGTTTCTGGTGTCTTCACAACCGCACATATTTAGCAATGGATATCGTGTCAGATTTGATGAAGAACCAGACCAGGATTGTGGTATTGGGTATGTAGTTCCTCAATGGGTTTCCGGAAAGCCTTACTTGTCAAGCATATGTGATGTGTATGGGTCTGAGAAGATCTTACCAACGACTACATTCATCCTTCCTCTAAAGCTTGACAAGGTGGAAGCGGTAAGAGCACAATTGTCAGAGCTGCACCCAGAGATTCTCTTGTTTTTGTCCAAGGTAAAGCGGCTATATGTACGCGGGTGTGATCCTGAAGAAGCTGATGATGTCTCCACAATTTCTATATTTAGTGAGACTGAACATATGAAGTTGAGTGATAAAACAGCGAAGTCACGATTTGTTCAACTTTCAGTCAAAGAAAAGATGTGTGATGCTGAGCAGCTGTGCAAGTATTACTTATGGAGAGAGGAATTTCCCGTAAACCCTAGAAATAAAGTTCGTATAAGGATGGATGTGGAGAAATGGGTGATTACGCTTGCTTTCCCATTTGGAGAAAGATTGAGAAGGGGAACATCATCTATTGGTATATTTGCTTTCCTGCCCACAGCGATGGTTACAAACTTTCCGTTTATAATTCAAGCAGATTTCATCCTTGCTTCTTCACGAGAATCTATAATGTTGGACAATGTATGGAATTTGGGAATTCTTAAATGTGTCCCTTCAGCTTTTGTGAATGCTTTTCAGTCTTGTGTGGAAGACTTCTCTCTGTTTCCTTCCATAAGTCAGCCATTTGAGTTTGTACCTGCACAAGATTCACCAATTCCTGAGTTTAGCAACCTAAGAGAGTCTATTAGAACTAACTTGCAAGATTTAAAGATAGTGCCCTGTCAAGTATTTTCTGGTAAGTGTTTATTCCTGCAGCCTCAATATGCACACCGGATCCTGCCAAAATTTAGGGACCTACTGGTTCAAATTAGAAGTGAAGGGGCTGTTTCAAGTGGTTCAGCTTCATTGAAGAGGGTCTTGCATCCATATCTGGATATTGAAAGATACAGTGCAGTTCTGGAGTTCCTAGGTGTGACATCTTCCAGTGGTGCTTGGTACACAAACTGCATCAACTCGTGCAATCTTGTTTTGTTATCTGATGAGGTCTATATTCAACTACTTGGTTTCATTGCTGATAATAAGGAAAAGTTCTCGAATCGTATTGACTGTCTACCTCTTATTAAATACATCAATCGGGAAGGGAATTCAGAATTGAGTACAATGACCAAAACCACCACAGCAGGGATGCTAAAGGTTCGATATTCTTTGGATGTAGAGCTTCACTCATGGCTGAACAAGTGCAATATGGAGCTTGGATGTCCTAATAACGTGTACTTGCTCCCCAATAGCACACAGAAAGCTCTTTTGAATTATCCAAAAATTGTTAATAATGAAAAGACAAATCATTTTAAAAGCTCATCTCTCTACAATTGGCTTTCATACCATGCAGGAGTGAAATTATTTACAACACATGATTATGCTTCCTTGCTCTGGAATCATGTATCAACTAAGGAACCATCTCTTGCAGTTACATTGGCCAGTTTCCTTTATCACGCCCACAAGAAATGTTTCCTTAATGATAGTCGGATACATTATTTCTGTGAAAGGATCCCAGTTATTGATGGGGCTGGCAATATTCAAATGCAAAGGACTGTAACTCTCGTCCATGCACTAGGAAGCAAATGGGTGAAGTTGTTTGGACCTCACAATCCTTTTCTGCAACAAAATTATGTGGATATAGGGGGTGTTTATTCCAAAAGTTGTATGTTCCTAGGAGAATCTACTCCTAGGAAAGAGCTTCTTAATTTTGTTTCCAAGTACAGTAAAGCTGTGGACTTACCCGAGCTGAGTCCTCCAGATGTAACGTTGCAGATAACATCTCATGAACTGTCAAGCGAACAGGCCTTTTTGCTGCTTGATTGGATCAGATTACTTCGAACTAGGGGTTCATGTTTACCTAGAAAGTTCATTGAAAGCATTCGAGATGGAAAATGGATGAAGACATATACAGGCTATGTTTCTCCAAGACGGGCCTTTCTTCCCAATGAAACAGGGAAAATTATTTTTGATATGATGAAGCATGTTATGGTGGATATCTCCATTATAGACGTTGAGTTTTATGAGAATCAAATCACTCTTTATCAAGATGAATTGAAATTTCTTGGTGTGGGGCTTGGATCAGAAGCTGTGTGGAAGCTGGTTTCTAATCACTTTAAGTCTTTCACTTCTCAGGGAATGAGCAAAGAGTTCAGCTTTGCTTTGCTGGATTTCATTAGTTTCTCTAAACGAAGAAGGATGGTGGATAAGGATTGGTTGTCTGTTATGAAGGAGAAGAAGTGGCTGAAGACTCATCATGGCTATGATGCTCCTAAGGGATCTATTCTCTTGCCATCTGACATAGAAGCTGAAGCATGTTCAAAAGTTACAAATCTTCCTATTGTTGATGAAGCATTCTATGGTAGCAGATTAAGATCATTCTTATCTGATTTAAGGCTACTTGAGGTTGAATATGACAGGGAGGAGGTGCAAAAATTAATTGCAGAGAATGTAATATTGACTCCAAATCTGTCTTCGATGACTGGTAGTTGTGGTTTATTGCTTCTTAAATGTATCAGATGTTTGGGAAGTGGAGCTGCTGGTTTGAATAATATGATTAAATGTCAACCTTGGATGAAGACGACTTTTGGTTTTAGATGCCCTGAAGAAACTGTCCTTATTGATCCTTGTTGGGGTTCTTTGTTGAGTGTCCTTCAGGTGCCTACCATAGATGAATTATACTATGGAAATGAAATGAGGCATTTCATTGATGAGTTGCAAGCCATTGGAGTGGTGGTTGGAAATGTTGGAGTAAGTAATACGATTGCTGCTCGCTTTAAATCAATCTTGTCTTCTTCTGGTCTGGCGCCTTCCAATGTCCTGTCTCTATTAGGTTGCATTAGAGAAATCAGTCAAACGATGTCTTTACAGTCCCCTGAATTGAATTGGTTGCTGTGTGAGAAATGGCTGAAAACACGTCATGGGTATAAGACTCCTAATGAATCCGTTATTTTCAGCTCTAAATGGGGATCAATCTTGTTGTATGTGGATCTCCCTCTCGTAGATGATATTTATTATGGTCTTGGCATATATAAGTTCAAGGATGAACTCCAGATGCTGGGTGCCATCACGGATTTTGAAGGAGGAGCTCTTTTTGTAGCTAAAGGTCTATGCAGCCCAATTGACCCTGAGCTAGTTTCTGCAGATGGATTTATATCATTGCTAGAGTGCATCAAGTCCCTGAAGTCAAGGTCTCTTGATGACCCCTTGCTGGGTGACTTTGTCAAGAACATTGCAAAAAGCAGGTGTCTAAAGATCAGGAATGGGTACAAAACGCCTGAAGAGTGTATTCTGTTTGATCCAGCCTGGGAAGGTATTCTGAAGCGATCAGATGCACCAACAATTGATGAAAGTTTTACTGGAACTGACATTTCTTTATACAAGAATCAGTTGAGAGATATTGGTGTGAAGGTAGATCCTCTCGAGGTTTGTTCTCTTCTTTCTAGGCTTCTTTTGTCACTGACAGACACGGCATATATAACAAGGATTTATAGCTTCCTCACTGAGTTCTTCTGGGGTCCACAAGTGCTAGATAATTGCAACTCTAAGGTTTGGATACCTGACCCAAAGGGTACTGGTGAATGGGTTAATTCTCTGGATTGCGTACTTCATGATAATAAAAATCTCTTTGGCTCTCGCTTTTTTTGTCTTGACAAGTTGTATAAGAAAGAGCTTCTCCCCATGTTTTGTTCAGCCTTTGGAGTTGCAGAACATCCTTCCATCAATGATTACTTGCAGCTATGGAACACATGGGCTATGAGGGATAACTTCCAAGTGACTGTTGAAGAGTGCAACTCGTTTTGGGGATGTGTATTTGACAATTGGAATCAACATGTAGAAGATATTCTGAAGGAGAAGTTGACCAAATTACCTGCCACGATTTCGTCCATGGTTGGGGAAATATATGTGATGAGCAGAGAGGAAGTATTCATTGGTGATGACTTGCAATTGAAAACTATCTTCTCAAGCTGTGATAAGGTTCCTTTGTTTGTGTGGTTTCCTAAGAGTAACAGCTCGTCTTTTATTCCTCCTAGAAGACTACGTAAAATTTATGATTGCCTTGGAGTTAAAAGAATTTCTCAATCAGTTGAGTATAATGTGAGTGGCATGGAATCATTAGATCATTTGGAAAAGGTTGAACCAAGAAATGAATTGATCAGAAGGGGTTTAATCAAAATTATTCTGGGTTTTCTTGCTGGTCCTTTGGTAGATATGCCATTGAAAGAGAGACACAAGGCTGCAAAGTTGATAAGCGTGCTGTCAGTTTATAAGAGTGACAAGCCAATTAAGGTCTGCTATCGACTAATGCCATCTGTGAGTACAACAGTGGAGGTAGAGAAAATAAAGCTGGTTCTTTGGGAGAAGAATTTGCAGCGATTACTGATTGATAGATTGGCCTATGAAGATGGAAAAGCTGATCTAGAATTTGTTACTTCTTTTGCTGATGAACTGGCGCAAGGACTGTTAGCGGATGCAAGACCCACAGCTGCAGATGCACTAAGCAAGATTATCCAAATGGGATTTATGTTTGATTTTATTGAGAATGAAGTTGATTTTTTGCTGATGAAAGAAAATCTAGAGTTATATATGGAAGATGAGCAGTTTCTTGATTCTGCTTTCGTCTCATCTAGAAAAACTGGTCTTGTTTATCGAAAGCGATCATGTAAGCAGTCGCAACAATTTGATCCTTCCACGCCAATGGTGTCTCGCAAGAAGCAGCGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1767

Amino Acids

200.53

Weight (kDa)

6.57

Isoelectric Point (pI)

39.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 59 - 167 1.2e-10 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 29, 1064, 4842
AarI CACCTGC 1 cut(s) 3807
Acc36I ACCTGC 3 cut(s) 1243, 3807, 4453
AccB1I GGYRCC 3 cut(s) 3232, 3379, 3635
AccB7I CCANNNNNTGG 1 cut(s) 3631
AccI GTMKAC 1 cut(s) 1648
AccII CGCG 1 cut(s) 732
AccIII TCCGGA 1 cut(s) 554
AciI CCGC 6 cut(s) 218, 464, 656, 718, 732, 5042
AclI AACGTT 1 cut(s) 2342
AcoI YGGCCR 1 cut(s) 2013
AcyI GRCGYC 1 cut(s) 3380
AfiI CCNNNNNNNGG 8 cut(s) 390, 1719, 1968, 3206, 3631, 3784, 3785, 4467
AflIII ACRYGT 3 cut(s) 1806, 3488, 4405
AhlI ACTAGT 1 cut(s) 155
AjiI CACGTC 1 cut(s) 3491
AjnI CCWGG 2 cut(s) 513, 3878
AjuI GAANNNNNNNTTGG 6 cut(s) 411, 443, 3031, 3063, 4488, 4520
AloI GAACNNNNNNTCC 2 cut(s) 341, 373
Alw21I GWGCWC 3 cut(s) 207, 667, 3664
Alw26I GTCTC 8 cut(s) 391, 766, 779, 3402, 3771, 4795, 5200, 5283
AlwNI CAGNNNCTG 2 cut(s) 2663, 3631
Ama87I CYCGRG 2 cut(s) 2318, 3995
Aor13HI TCCGGA 1 cut(s) 554
AoxI GGCC 5 cut(s) 373, 2013, 2379, 2508, 4968
AseI ATTAAT 1 cut(s) 3011
Asp700I GAANNNNTTC 4 cut(s) 2045, 2454, 3888, 4416
AspA2I CCTAGG 3 cut(s) 1504, 2248, 2264
AspLEI GCGC 2 cut(s) 3382, 5029
AspS9I GGNCC 6 cut(s) 87, 1390, 2177, 2508, 4091, 4772
AsuC2I CCSGG 1 cut(s) 289
AsuHPI GGTGA 6 cut(s) 970, 1239, 2176, 3800, 4163, 4520
AsuII TTCGAA 1 cut(s) 2413
AvaI CYCGRG 2 cut(s) 2318, 3995
AvaII GGWCC 5 cut(s) 87, 1390, 2177, 4091, 4772
AvrII CCTAGG 3 cut(s) 1504, 2248, 2264
AxyI CCTNAGG 1 cut(s) 2847
BaeGI GKGCMC 1 cut(s) 1317
BalI TGGCCA 1 cut(s) 2015
BamHI GGATCC 2 cut(s) 1371, 2085
BanI GGYRCC 3 cut(s) 3232, 3379, 3635
BanII GRGCYC 2 cut(s) 207, 3664
BauI CACGAG 2 cut(s) 1095, 1552
BbsI GAAGAC 9 cut(s) 60, 447, 1194, 2483, 2828, 3039, 3158, 3357, 4615
Bbv12I GWGCWC 3 cut(s) 207, 667, 3664
BceAI ACGGC 2 cut(s) 360, 4059
BciT130I CCWGG 2 cut(s) 515, 3880
BciVI GTATCC 2 cut(s) 2058, 4122
BclI TGATCA 1 cut(s) 4728
BcnI CCSGG 1 cut(s) 289
BcoDI GTCTC 8 cut(s) 391, 766, 779, 3402, 3771, 4795, 5200, 5283
BcuI ACTAGT 1 cut(s) 155
BfmI CTRYAG 3 cut(s) 1349, 3714, 5061
BfoI RGCGCY 1 cut(s) 3383
BfuAI ACCTGC 3 cut(s) 1243, 3807, 4453
BfuI GTATCC 2 cut(s) 2058, 4122
BglII AGATCT 1 cut(s) 603
BlnI CCTAGG 3 cut(s) 1504, 2248, 2264
BlpI GCTNAGC 1 cut(s) 867
Bme1390I CCNGG 3 cut(s) 289, 515, 3880
Bme18I GGWCC 5 cut(s) 87, 1390, 2177, 4091, 4772
BmeT110I CYCGRG 2 cut(s) 2318, 3995
BmgBI CACGTC 1 cut(s) 3491
BmgT120I GGNCC 6 cut(s) 87, 1390, 2177, 2508, 4091, 4772
BmrFI CCNGG 3 cut(s) 289, 515, 3880
BmrI ACTGGG 1 cut(s) 2084
BmuI ACTGGG 1 cut(s) 2084
BpiI GAAGAC 9 cut(s) 60, 447, 1194, 2483, 2828, 3039, 3158, 3357, 4615
BplI GAGNNNNNCTC 4 cut(s) 1745, 1777, 4352, 4384
BpmI CTGGAG 4 cut(s) 315, 1517, 2316, 3608
Bpu10I CCTNAGC 2 cut(s) 103, 3702
Bpu1102I GCTNAGC 1 cut(s) 867
Bpu14I TTCGAA 1 cut(s) 2413
BpuEI CTTGAG 2 cut(s) 2996, 4522
BpuMI CCSGG 1 cut(s) 289
Bsa29I ATCGAT 1 cut(s) 354
BsaAI YACGTR 1 cut(s) 4616
BsaBI GATNNNNATC 2 cut(s) 2947, 3727
BsaHI GRCGYC 1 cut(s) 3380
BsaI GGTCTC 1 cut(s) 3771
BsaJI CCNNGG 8 cut(s) 708, 1504, 2248, 2264, 3143, 3879, 4461, 4633
BsaWI WCCGGW 2 cut(s) 554, 1368
BsaXI ACNNNNNCTCC 4 cut(s) 2798, 2828, 3294, 3324
Bsc4I CCNNNNNNNGG 8 cut(s) 390, 1719, 1968, 3206, 3631, 3784, 3785, 4467
Bse1I ACTGG 9 cut(s) 1401, 1521, 2016, 2090, 3061, 3937, 4153, 5028, 5215
Bse21I CCTNAGG 1 cut(s) 2847
Bse3DI GCAATG 4 cut(s) 484, 1605, 3731, 3804
Bse8I GATNNNNATC 2 cut(s) 2947, 3727
BseAI TCCGGA 1 cut(s) 554
BseBI CCWGG 2 cut(s) 515, 3880
BseCI ATCGAT 1 cut(s) 354
BseDI CCNNGG 8 cut(s) 708, 1504, 2248, 2264, 3143, 3879, 4461, 4633
BseJI GATNNNNATC 2 cut(s) 2947, 3727
BseLI CCNNNNNNNGG 8 cut(s) 390, 1719, 1968, 3206, 3631, 3784, 3785, 4467
BseMI GCAATG 4 cut(s) 484, 1605, 3731, 3804
BseMII CTCAG 7 cut(s) 588, 858, 1248, 2315, 2720, 3693, 4068
BseNI ACTGG 9 cut(s) 1401, 1521, 2016, 2090, 3061, 3937, 4153, 5028, 5215
BseRI GAGGAG 2 cut(s) 3011, 3672
BseSI GKGCMC 1 cut(s) 1317
BseYI CCCAGC 2 cut(s) 3631, 3784
BsgI GTGCAG 3 cut(s) 665, 1221, 1509
Bsh1236I CGCG 1 cut(s) 732
BshFI GGCC 5 cut(s) 375, 2015, 2381, 2510, 4970
BshNI GGYRCC 3 cut(s) 3232, 3379, 3635
BshVI ATCGAT 1 cut(s) 354
BsiHKAI GWGCWC 3 cut(s) 207, 667, 3664
BsiHKCI CYCGRG 2 cut(s) 2318, 3995
BsiSI CCGG 3 cut(s) 288, 555, 1369
BslFI GGGAC 4 cut(s) 1133, 1403, 3430, 3736
BslI CCNNNNNNNGG 8 cut(s) 390, 1719, 1968, 3206, 3631, 3784, 3785, 4467
BsmAI GTCTC 8 cut(s) 391, 766, 779, 3402, 3771, 4795, 5200, 5283
BsmBI CGTCTC 1 cut(s) 5200
BsmFI GGGAC 4 cut(s) 1133, 1403, 3430, 3736
BsmI GAATGC 4 cut(s) 220, 1171, 2454, 2927
BsnI GGCC 5 cut(s) 375, 2015, 2381, 2510, 4970
Bso31I GGTCTC 1 cut(s) 3771
BsoBI CYCGRG 2 cut(s) 2318, 3995
Bsp119I TTCGAA 1 cut(s) 2413
Bsp1286I GDGCHC 4 cut(s) 207, 667, 1317, 3664
Bsp13I TCCGGA 1 cut(s) 554
Bsp1720I GCTNAGC 1 cut(s) 867
Bsp19I CCATGG 1 cut(s) 4461
BspACI CCGC 6 cut(s) 218, 464, 656, 718, 732, 5042
BspANI GGCC 5 cut(s) 375, 2015, 2381, 2510, 4970
BspCNI CTCAG 7 cut(s) 589, 859, 1249, 2316, 2719, 3694, 4069
BspDI ATCGAT 1 cut(s) 354
BspEI TCCGGA 1 cut(s) 554
BspFNI CGCG 1 cut(s) 732
BspHI TCATGA 2 cut(s) 2359, 4183
BspMAI CTGCAG 3 cut(s) 1353, 3718, 5065
BspMI ACCTGC 3 cut(s) 1243, 3807, 4453
BspT104I TTCGAA 1 cut(s) 2413
BspT107I GGYRCC 3 cut(s) 3232, 3379, 3635
BspTNI GGTCTC 1 cut(s) 3771
BsrDI GCAATG 4 cut(s) 484, 1605, 3731, 3804
BsrI ACTGG 9 cut(s) 1401, 1521, 2016, 2090, 3061, 3937, 4153, 5028, 5215
BssECI CCNNGG 8 cut(s) 708, 1504, 2248, 2264, 3143, 3879, 4461, 4633
BssNI GRCGYC 1 cut(s) 3380
BssSI CACGAG 2 cut(s) 1095, 1552
BssT1I CCWWGG 7 cut(s) 708, 1504, 2248, 2264, 3143, 4461, 4633
Bst2BI CACGAG 2 cut(s) 1095, 1552
Bst2UI CCWGG 2 cut(s) 515, 3880
Bst6I CTCTTC 6 cut(s) 72, 389, 1442, 3849, 4014, 4353
BstACI GRCGYC 1 cut(s) 3380
BstBAI YACGTR 1 cut(s) 4616
BstBI TTCGAA 1 cut(s) 2413
BstC8I GCNNGC 6 cut(s) 170, 969, 2107, 3294, 3346, 4829
BstDSI CCRYGG 1 cut(s) 4461
BstFNI CGCG 1 cut(s) 732
BstH2I RGCGCY 1 cut(s) 3383
BstHHI GCGC 2 cut(s) 3382, 5029
BstMAI GTCTC 8 cut(s) 391, 766, 779, 3402, 3771, 4795, 5200, 5283
BstMWI GCNNNNNNNGC 3 cut(s) 372, 662, 5066
BstNI CCWGG 2 cut(s) 515, 3880
BstNSI RCATGY 3 cut(s) 2561, 2892, 4409
BstSCI CCNGG 3 cut(s) 287, 513, 3878
BstSFI CTRYAG 3 cut(s) 1349, 3714, 5061
BstSLI GKGCMC 1 cut(s) 1317
BstSNI TACGTA 1 cut(s) 4616
BstUI CGCG 1 cut(s) 732
BstV2I GAAGAC 9 cut(s) 60, 447, 1194, 2483, 2828, 3039, 3158, 3357, 4615
BstX2I RGATCY 6 cut(s) 603, 1371, 2085, 2852, 3562, 3988
BstXI CCANNNNNNTGG 4 cut(s) 521, 1042, 3304, 5272
BstYI RGATCY 6 cut(s) 603, 1371, 2085, 2852, 3562, 3988
Bsu15I ATCGAT 1 cut(s) 354
Bsu36I CCTNAGG 1 cut(s) 2847
BsuI GTATCC 2 cut(s) 2058, 4122
BsuRI GGCC 5 cut(s) 375, 2015, 2381, 2510, 4970
BsuTUI ATCGAT 1 cut(s) 354
BtgI CCRYGG 1 cut(s) 4461
BtgZI GCGATG 1 cut(s) 1055
BtrI CACGTC 1 cut(s) 3491
BtsIMutI CAGTG 5 cut(s) 1492, 1528, 4031, 4074, 4905
BveI ACCTGC 3 cut(s) 1243, 3807, 4453
Cac8I GCNNGC 6 cut(s) 170, 969, 2107, 3294, 3346, 4829
CaiI CAGNNNCTG 2 cut(s) 2663, 3631
CciI TCATGA 2 cut(s) 2359, 4183
CfoI GCGC 2 cut(s) 3382, 5029
Cfr13I GGNCC 6 cut(s) 87, 1390, 2177, 2508, 4091, 4772
ClaI ATCGAT 1 cut(s) 354
CseI GACGC 1 cut(s) 65
DinI GGCGCC 1 cut(s) 3381
DraI TTTAAA 3 cut(s) 1305, 1888, 3352
EaeI YGGCCR 1 cut(s) 2013
Eam1104I CTCTTC 6 cut(s) 72, 389, 1442, 3849, 4014, 4353
EarI CTCTTC 6 cut(s) 72, 389, 1442, 3849, 4014, 4353
Ecl136II GAGCTC 2 cut(s) 205, 3662
Eco105I TACGTA 1 cut(s) 4616
Eco130I CCWWGG 7 cut(s) 708, 1504, 2248, 2264, 3143, 4461, 4633
Eco147I AGGCCT 1 cut(s) 2381
Eco24I GRGCYC 2 cut(s) 207, 3664
Eco31I GGTCTC 1 cut(s) 3771
Eco32I GATATC 2 cut(s) 485, 2572
Eco47I GGWCC 5 cut(s) 87, 1390, 2177, 4091, 4772
Eco53kI GAGCTC 2 cut(s) 205, 3662
Eco81I CCTNAGG 1 cut(s) 2847
Eco88I CYCGRG 2 cut(s) 2318, 3995
EcoICRI GAGCTC 2 cut(s) 205, 3662
EcoO109I RGGNCCY 2 cut(s) 87, 1390
EcoRI GAATTC 2 cut(s) 1134, 1681
EcoRII CCWGG 2 cut(s) 513, 3878
EcoRV GATATC 2 cut(s) 485, 2572
EcoT14I CCWWGG 7 cut(s) 708, 1504, 2248, 2264, 3143, 4461, 4633
EcoT38I GRGCYC 2 cut(s) 207, 3664
EgeI GGCGCC 1 cut(s) 3381
EheI GGCGCC 1 cut(s) 3381
ErhI CCWWGG 7 cut(s) 708, 1504, 2248, 2264, 3143, 4461, 4633
Esp3I CGTCTC 1 cut(s) 5200
FaqI GGGAC 4 cut(s) 1133, 1403, 3430, 3736
FauI CCCGC 1 cut(s) 725
FauNDI CATATG 3 cut(s) 362, 578, 795
FbaI TGATCA 1 cut(s) 4728
FblI GTMKAC 1 cut(s) 1648
FriOI GRGCYC 2 cut(s) 207, 3664
GlaI GCGC 2 cut(s) 3381, 5028
GsaI CCCAGC 2 cut(s) 3635, 3788
GsuI CTGGAG 4 cut(s) 315, 1517, 2316, 3608
HaeII RGCGCY 1 cut(s) 3383
HaeIII GGCC 5 cut(s) 375, 2015, 2381, 2510, 4970
HapII CCGG 3 cut(s) 288, 555, 1369
HgaI GACGC 1 cut(s) 65
HhaI GCGC 2 cut(s) 3382, 5029
Hin1I GRCGYC 1 cut(s) 3380
Hin6I GCGC 2 cut(s) 3380, 5027
HinP1I GCGC 2 cut(s) 3380, 5027
HincII GTYRAC 2 cut(s) 3140, 4434
HindII GTYRAC 2 cut(s) 3140, 4434
HindIII AAGCTT 2 cut(s) 21, 638
HpaII CCGG 3 cut(s) 288, 555, 1369
HphI GGTGA 6 cut(s) 970, 1239, 2176, 3800, 4163, 4520
Hpy166II GTNNAC 9 cut(s) 913, 1536, 1649, 1810, 2311, 2432, 3140, 4094, 4434
Hpy8I GTNNAC 9 cut(s) 913, 1536, 1649, 1810, 2311, 2432, 3140, 4094, 4434
HpyCH4IV ACGT 5 cut(s) 1806, 2342, 2586, 3490, 4615
HpyF10VI GCNNNNNNNGC 3 cut(s) 372, 662, 5066
HpySE526I ACGT 5 cut(s) 1806, 2342, 2586, 3490, 4615
Hsp92I GRCGYC 1 cut(s) 3380
HspAI GCGC 2 cut(s) 3380, 5027
KasI GGCGCC 1 cut(s) 3379
Kpn2I TCCGGA 1 cut(s) 554
Ksp22I TGATCA 1 cut(s) 4728
LmnI GCTCC 8 cut(s) 14, 202, 296, 1786, 1821, 2848, 3107, 3659
MaeII ACGT 5 cut(s) 1806, 2342, 2586, 3490, 4615
MfeI CAATTG 6 cut(s) 668, 1906, 3693, 3915, 4393, 4520
MflI RGATCY 6 cut(s) 603, 1371, 2085, 2852, 3562, 3988
MhlI GDGCHC 4 cut(s) 207, 667, 1317, 3664
MlsI TGGCCA 1 cut(s) 2015
MluNI TGGCCA 1 cut(s) 2015
Mly113I GGCGCC 1 cut(s) 3380
MlyI GAGTC 6 cut(s) 110, 1286, 2335, 2818, 3028, 3499
MmeI TCCRAC 4 cut(s) 1092, 2042, 3292, 3301
Mox20I TGGCCA 1 cut(s) 2015
MroI TCCGGA 1 cut(s) 554
MroXI GAANNNNTTC 4 cut(s) 2045, 2454, 3888, 4416
MscI TGGCCA 1 cut(s) 2015
MslI CAYNNNNRTG 5 cut(s) 1040, 1953, 2835, 3302, 5270
Msp20I TGGCCA 1 cut(s) 2015
MspA1I CMGCKG 2 cut(s) 874, 5060
MspI CCGG 3 cut(s) 288, 555, 1369
MspR9I CCNGG 3 cut(s) 289, 515, 3880
MunI CAATTG 6 cut(s) 668, 1906, 3693, 3915, 4393, 4520
Mva1269I GAATGC 4 cut(s) 220, 1171, 2454, 2927
MvaI CCWGG 2 cut(s) 515, 3880
MvnI CGCG 1 cut(s) 732
MwoI GCNNNNNNNGC 3 cut(s) 372, 662, 5066
NarI GGCGCC 1 cut(s) 3380
NciI CCSGG 1 cut(s) 289
NcoI CCATGG 1 cut(s) 4461
NdeI CATATG 3 cut(s) 362, 578, 795
NmuCI GTSAC 7 cut(s) 151, 822, 1510, 3788, 4029, 4348, 4847
NspI RCATGY 3 cut(s) 2561, 2892, 4409
NspV TTCGAA 1 cut(s) 2413
PaeR7I CTCGAG 1 cut(s) 3995
PagI TCATGA 2 cut(s) 2359, 4183
PaqCI CACCTGC 1 cut(s) 3807
PceI AGGCCT 1 cut(s) 2381
PciI ACATGT 1 cut(s) 4405
PctI GAATGC 4 cut(s) 220, 1171, 2454, 2927
PdmI GAANNNNTTC 4 cut(s) 2045, 2454, 3888, 4416
PflFI GACNNNGTC 1 cut(s) 3794
PflMI CCANNNNNTGG 1 cut(s) 3631
PleI GAGTC 6 cut(s) 110, 1285, 2334, 2818, 3028, 3499
PluTI GGCGCC 1 cut(s) 3383
PpsI GAGTC 6 cut(s) 110, 1285, 2334, 2818, 3028, 3499
Ppu21I YACGTR 1 cut(s) 4616
PpuMI RGGWCCY 2 cut(s) 87, 1390
PscI ACATGT 1 cut(s) 4405
PshBI ATTAAT 1 cut(s) 3011
PsiI TTATAA 3 cut(s) 29, 1064, 4842
Psp124BI GAGCTC 2 cut(s) 207, 3664
Psp1406I AACGTT 1 cut(s) 2342
Psp5II RGGWCCY 2 cut(s) 87, 1390
Psp6I CCWGG 2 cut(s) 513, 3878
PspFI CCCAGC 2 cut(s) 3631, 3784
PspGI CCWGG 2 cut(s) 513, 3878
PspPI GGNCC 6 cut(s) 87, 1390, 2177, 2508, 4091, 4772
PspPPI RGGWCCY 2 cut(s) 87, 1390
PstI CTGCAG 3 cut(s) 1353, 3718, 5065
PstNI CAGNNNCTG 2 cut(s) 2663, 3631
PsuI RGATCY 6 cut(s) 603, 1371, 2085, 2852, 3562, 3988
PsyI GACNNNGTC 1 cut(s) 3794
PvuII CAGCTG 2 cut(s) 874, 5060
RseI CAYNNNNRTG 5 cut(s) 1040, 1953, 2835, 3302, 5270
SacI GAGCTC 2 cut(s) 207, 3664
Sau96I GGNCC 6 cut(s) 87, 1390, 2177, 2508, 4091, 4772
SchI GAGTC 6 cut(s) 110, 1286, 2335, 2818, 3028, 3499
ScrFI CCNGG 3 cut(s) 289, 515, 3880
SduI GDGCHC 4 cut(s) 207, 667, 1317, 3664
SfcI CTRYAG 3 cut(s) 1349, 3714, 5061
SfoI GGCGCC 1 cut(s) 3381
Sfr274I CTCGAG 1 cut(s) 3995
SfuI TTCGAA 1 cut(s) 2413
SinI GGWCC 5 cut(s) 87, 1390, 2177, 4091, 4772
SlaI CTCGAG 1 cut(s) 3995
SmiMI CAYNNNNRTG 5 cut(s) 1040, 1953, 2835, 3302, 5270
SmlI CTYRAG 3 cut(s) 2975, 3995, 4537
SmoI CTYRAG 3 cut(s) 2975, 3995, 4537
SnaBI TACGTA 1 cut(s) 4616
SpeI ACTAGT 1 cut(s) 155
SseBI AGGCCT 1 cut(s) 2381
SsiI CCGC 6 cut(s) 218, 464, 656, 718, 732, 5042
SspDI GGCGCC 1 cut(s) 3379
SspI AATATT 2 cut(s) 2113, 3030
SstI GAGCTC 2 cut(s) 207, 3664
StuI AGGCCT 1 cut(s) 2381
StyD4I CCNGG 3 cut(s) 287, 513, 3878
StyI CCWWGG 7 cut(s) 708, 1504, 2248, 2264, 3143, 4461, 4633
TaiI ACGT 5 cut(s) 1809, 2345, 2589, 3493, 4618
TaqI TCGA 9 cut(s) 354, 1631, 1735, 2413, 2458, 3050, 3996, 4873, 5224
TatI WGTACW 5 cut(s) 231, 1694, 1809, 2295, 4892
TauI GCSGC 1 cut(s) 721
TscAI CASTG 5 cut(s) 1492, 1528, 4038, 4081, 4905
TseFI GTSAC 7 cut(s) 151, 822, 1510, 3788, 4029, 4348, 4847
Tsp45I GTSAC 7 cut(s) 151, 822, 1510, 3788, 4029, 4348, 4847
TspGWI ACGGA 3 cut(s) 1047, 3508, 3659
TspRI CASTG 5 cut(s) 1492, 1528, 4038, 4081, 4905
Tth111I GACNNNGTC 1 cut(s) 3794
Van91I CCANNNNNTGG 1 cut(s) 3631
VpaK11BI GGWCC 5 cut(s) 87, 1390, 2177, 4091, 4772
VspI ATTAAT 1 cut(s) 3011
XbaI TCTAGA 3 cut(s) 4993, 5149, 5201
XceI RCATGY 3 cut(s) 2561, 2892, 4409
XcmI CCANNNNNNNNNTGG 1 cut(s) 3304
XhoI CTCGAG 1 cut(s) 3995
XmaJI CCTAGG 3 cut(s) 1504, 2248, 2264
XmiI GTMKAC 1 cut(s) 1648
XmnI GAANNNNTTC 4 cut(s) 2045, 2454, 3888, 4416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.