Rh4CG182600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003946
Physical Location & Seq
Reverse (-)
39430347 .. 39437744
7398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG182600.1

Sequence Viewer

Length: 5274 bp
ATGGGAAATCCCAGCATTGAAGCTATGCAACACATAGAAGAGATCAGGACAAAGAAGTTCTCAATAGGGAAGAAAGAACCAAACCCCTTAACTCTTGATCTTCACCATGCTGTGACCAGTCTATCTGCAGAGCTGTATCAGAAAGACATCCATTTCCTGATGGAGCTCATACAGAATGCTGAAGACAATGAGTACAAAGAAGGTGTAGAACCGACTCTGGAGTTTGTGCTGACCGAGAAAGACATAACTGGGACTGGAGCACCGGCTACTCTTTTGGTTTTCAATAATGAGGTTGGCTTTTCCAGGGAGAATATAGACTCCATTTGCAGCATTGGCCGTTCTACCAAGAAAGGGAAGAGACGTCAGGGCTTTATTGGAGAGAAAGGAATTGGATTCAAAAGTGTGTTTCTTGTGAGTTCACAACCTCACATATTTAGCAATGGATATCATGTCAAATTCAGGGAAGAACCAAACCAGGTCTGTGGTATTGGTTATATAGTTCCTGAATGGTTTAGGGCTGCAGATTTGTCAGTATTAATGGTTCAGGCTGTGTATGGGCACGGCAAGGACTTGCCAACAACTACATTCGTACTTCCTCTAAAGCGAGACAAGGTGGAAGCAGTAAGAGCACAACTGTTGGAGCTGCACCCGGAGATTCTCCTGTTCTTATCCAAAATAAAGCGGCTATATGTACGTGGGTGTGATCCTCAAAAAGCTGATGATGTCTCAACATTATCTATATTTAGTGAGACTGAACATACAGAGTTAAGGAGTAAAACAGCCAATTCTCGTGTTGTGCAACTTTCTGTTAAGGAAAAAATGTGTGATACTGAGCAGTTGTGCAAGTATTACTTGTGGAGAGAGGAGTTTCCAGTGAAACCTGGGAATAGAGTTGAAATAAGGATGGATGTGGAGAAATGGGTGATTACTCTTGCTTTCCCATTTGGAAAAAGGCTGCGAAGAGGAACGTCTCCTGTTGGTATATTTGCTTTCCTGCCAACAACAATGGTCACAAACTTCCCCTTTGTAGTTCAAGCTGACTTCATCCTTGCTTCTTCACGCGAATCCATAATTTTGGACAATTCATGGAACATGGGAATTCTTCAATGTGTCCCATCAGCCTTTGTGAATGCATTTCAACATTGCGTGGGACAACTGAACCTCTTTTCTTCTGTAAGTCAGCCATTTGAGTTTTTACCTGCTCAAAACTCGCCAACACCAGAGTTTAATACAGTACGAGAGTCCATTAGGACTAGGCTGCAAGGTCTAAACATAGTGCCCTGTAAAAGATTTTTTGGTGATAGTCTTTCCTCCAAGCTGCCTCGACGTGTAGTACGGATCAAGCCAAAATTTAGGGACCTGGTTGTTAAAATTAAAGCGGAAGGTGCTGTTTTTGGCAGTTCATCTTCATCTTCATTGAAGAGGGTCTTGGATTCATCTCTGGATGTTGAAAAATACAGGGACATTATGAACTTTCTAGGTGTGGCATATGCTGATGATGATTGGTACACAGCCTGCATCAACTCTTGTAATCTTTTGTTGTTATCAGATGAAGTCTACATTGAACTACTTGGTTTTTTGGCTGATAATGATGCAGAGTTTGACTCAGATAGCATTGAGACCATACCTCTTCTTAAGTATACTGATCGGAAAGGGAGTCTGGCCTTGTGTACAATTAAAACTACAGAAGTGGAACTCAAGATTCGATATGCTATGGGCCTGGAGCTTCACACATGGCTCAGCAAGTGCAATATGGAGTTTGGCTGTCCTGATAATGTGTATTTCTTACCAAATAAGACACTGGAAGCTGTTTTGAATCGCCAGAAAAGTCATCCCCCAAAGCGACGTTATAGAAAGAAAAATTACATGGAGAGTTCATCTGTCTGCAATTGGCTGAATAGAAGTGCTGGAGTGAAATCTCTTTCAGCATATGATTATGCTTTCTTACTTTTGAATCATGTATCAAAGAAGGAATCCAATGTTGCAGTCAGATTATCCAATTTCCTTTATCTTGCCCACAAGGAAGGCTTCCTCACTGAATATCAAATATCTGATCTTTGTGGTAGGATTCCAATTGTTGATGGGTCTGATAAAGTAAGAGTGCAAAGAACCGTAACTTTGGTCCCTTCATCAGGGAGCAAATGGGCCAAATTGTTTGGACTTCAAAATCCATTTGCTGAACAACATTATGTTGATATAGGGGATGTTTATGCTAAAAGCAGTTTGTTTCTTGGGAAATATTCTACCCCTGAGAAAGAGCTTCTGCATTTTGTTGGCGAGTATAGCAAAGCTACGGACTTACCCAACTTGTGTCCTCCTGATGTGGTGTTACAGATCGCATCTCATGAACTCTCGAGTGAGCAGGCCTTTTTACTCCTTGATTGGATCAGGTTACTGCGAACCAAGAATTCATCCATACCCAAAAACTTCATTGAAAGCATTGAAAATGGAAAGTGGATGAAGACATTCTCAGGTTATAAATCTCCACAAGAGGTCATTTTTCCCGATGAAACAGGAAAAGTTCTTTTTGATTTGATGAAGCATGTTCTGGAGGATGTTGCTATTTTGGACCAAGAATTTTATATGAATAAAATCAAACTTTACCAAGATGAACTGAAATTTCTTGGTGTGCGGCTTGGATCTGAGAATATAGGGAAGCTGGTCACCACTCGCTTTTTGTCTCTCGCATCTTCTGGAATGAGCAAAGAATCCACATATTCTTTGCTAACTTTCATTAGCTTCTCTAGAGGGAGGAACATGGTTGATAATGGTTGGTTGGCTGTTATGAAGGAGAAGAAGTGGCTGAAGACGCTCCGAGGTTACAATCCTCCCAACGGATCTATTCTTTTGCTGCAATCTGAGATAGAAGTTGAAAACTGTTCAAAGGTTACAAATCTTCCGATTGTTGATGAAGCATTCTATGGAAGCAGATTAGGTTCATTCTTATCTGAGTTGAGGCTACTTGGAGTGACATATGATGTGGATGTGCTCCAAAGATTGGTTGCAGAGAACGTAAATCTAACTTCAAATCTGTCTTCTATGACTGGTGGTTGTGGTTTGCTGATTCTTAAATGTGTTAGATGGTTGGGATCTGGAGCTGCAGGCTTGATTAACAGGATAAAATGCCATCCTTGGGTAAAGACAACTCTAGGTTTCAAAAGCCCTTCGGAAACTGTTCTTCCTGATCCTAAGTGGGTTTCTTTGCTCAGTACTCTTCAAGTCCCTGCCATAGATGAATTGTATTATGACAATGCAATTAGGCAATTTGTTGATGAATTGAATGCATTGGGAGTGGTAGTTGATAACACTGGAACAACTGTGATGATAGCTGCTCGTTTTAAATCCCTATTGTCTTCCTCTAGCTTAGATCCTGCCACTGTTATGGCGCTGTTACGTTGCATCAGAGAAATGAATCTAACTATGTCTTTACAACACTCTGAACTGCAGTGGCTGCTGTCTGAAAAATGGTTAAAAACTCGGCATGGATTCAAGACTCCTGATGAATCAATTATTTTCAGCTCTAAATGGGGACAATTGATGTTGTTTGTTGATCTTCCTCTAATTGATGACGTGTACTATGGTACTGGCATATATAAGTTTAGGGATGAGCTAGAGAAGTTGGGTGCCATCACCGATTTTGAACAAGGAGCTCTATTTGTAGCTAAAGGTCTACACAGTCACAGTCCTATTGAAGCTGAGCTACTTGATGCAGATGGTATTAGATCATTGCTAGAGTGCATAAAGTCTCTGATGTCCCGATCGCTTGATGATCCCTTAGTGGGAGATTTTCTGAAGAACATTGCAAACAGCAGGTGTTTGAAGACCACAAACGGTTATAAAGTGCCAGAAGACTGCATTCTATTTGATTCATCCTGGGAATGCATTCTGAACAGGTTGGATGCACCAAGCATTGATGAAGCATTTTACAGAACTGACATTTCTGCTTACAAGAATCAATTGAGAGATGTTGGTGTGAAGGTAGATCCTCAGGATGTTTGTTCTCTTCTTTCTGGTCATCTTTTGTCACTGACAGACACAGCTTCAATAACAAGGATATATAGCTTCCTCAACAAGTTCCAGTGGAGTCCTAAAGTTCTAAATAAGTGCAACTCTCAGGTGTGGATTCCTGAACCAAAGAGTAAGGCAGGGGCATGGGCATGGGCAAAATCTCAATGTTGTGTACTTCATGATAAGAAAAATCTGTTTGGCTCTTGTTTGTTCAGTCTGGACAAATCTTACAGTAAAGAGCTTCTCCCCATTTTTTCTACAGCCTTTGGAGTTCCTGAAAATCCATCTATCAAGGATTACTTGCTGCTATGGGACATTTGGGCCTCAATGAACAACTCTAAAGTGACAGTTGAAAAATGCAGCTCGTTTTGGAAATGTATTGTTGACAACTGGAATCCACACATTGAAGAGACCCTAAAACAGAACTTAACAAAAGTACCTGCCACAATGTCCATGTCGGGGCAAATATATCTGATCAGAAAAGAGGAAGCATTCATTGCTGATGATTTGCAACTGAAAACAATTTTTTCAAGCTTTGATAAGGCACCTCTGTTTGTCTGGTTTCCTAATAGCTCGTCTTCTGTTCCTCATAGAAGACTTCTTGAAATTTATGAATCTCTTGGAGTTAGAAAGATCTCTGCATCTGTTAAGTGCACTGTGAAGGGCATGCTATCATCAGAGCATTGGGAAAAGGTCGAACAAAATAATGGATTGATTGGCAGGAGTTTGACCAAAATTGTTCTGGCTTTTCTTGCTGGTCCTGAGCTGAATATGCCGTTCAAAGAGAGACTTGAGGCTGCAAAATCGATGAGTGTGCTGACAGTGTATAAGACTGACAAACCAATTAAAGTCTGTTATCGGCTAATGCCATGCGCAAGTACAACAGTGGAAGTGGAAAAACTGAAGCTGGTTCTTTGGGAGAAGGATTCTAAGCGGCTGTTCATTGATAAATCAGGTTATGATGGAGGAAAATGTGATTTAGAATTCGTTACTTCTTTTGCCAATGAACTGGCACAAGGACTACTAGCACAGGCAAGACCCGCAGCTGCACATGCATTAAGCAGAATTATCCAAATGGCATACATATATGGTTTTAATGAGAATGAAGTGGAGTTTTTGCTGATGACTGAAAACTTAGAGTTATTTGTGGAAGATAAGAAGTTTCTTGATGATGCATATCCTGTTAGCCAAAAGCGCACAAGTAAGAAGCTAGAGCAATTAGGTCCCTCAACACCAATGCCTACCTGCAAGAAGCAGCGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1757

Amino Acids

198.54

Weight (kDa)

8.09

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 46 - 150 3.4e-10 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2478, 3837
AarI CACCTGC 1 cut(s) 3801
AatII GACGTC 1 cut(s) 364
Acc16I TGCGCA 1 cut(s) 4882
Acc36I ACCTGC 4 cut(s) 1207, 3801, 4456, 5261
AccB1I GGYRCC 2 cut(s) 3623, 4552
AccB7I CCANNNNNTGG 1 cut(s) 2998
AccI GTMKAC 3 cut(s) 1557, 1640, 3670
AccII CGCG 1 cut(s) 1062
AciI CCGC 5 cut(s) 682, 1379, 2632, 4942, 5049
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 8 cut(s) 455, 1098, 1349, 2407, 2576, 2618, 4614, 4991
AcuI CTGAAG 4 cut(s) 201, 2825, 3812, 4931
AcyI GRCGYC 1 cut(s) 361
AfiI CCNNNNNNNGG 7 cut(s) 351, 2132, 2834, 2998, 3133, 3779, 4467
AflII CTTAAG 1 cut(s) 1634
AflIII ACRYGT 2 cut(s) 1327, 3570
AjiI CACGTC 2 cut(s) 1328, 3571
AjnI CCWGG 6 cut(s) 302, 474, 880, 1359, 1719, 3872
AjuI GAANNNNNNNTTGG 2 cut(s) 1412, 1444
Alw21I GWGCWC 6 cut(s) 168, 262, 631, 2991, 3652, 4664
Alw44I GTGCAC 1 cut(s) 4660
AlwNI CAGNNNCTG 1 cut(s) 3451
Ama87I CYCGRG 1 cut(s) 2353
AoxI GGCC 6 cut(s) 334, 1662, 1717, 2145, 2364, 4329
ApaLI GTGCAC 1 cut(s) 4660
ApoI RAATTY 8 cut(s) 455, 1098, 1349, 2407, 2576, 2618, 4614, 4991
AseI ATTAAT 1 cut(s) 536
Asp700I GAANNNNTTC 3 cut(s) 2465, 3174, 3882
AspLEI GCGC 4 cut(s) 3388, 4883, 5205, 5268
AspS9I GGNCC 8 cut(s) 1357, 1717, 2122, 2145, 2569, 4329, 4766, 5231
AsuC2I CCSGG 1 cut(s) 650
AsuHPI GGTGA 5 cut(s) 95, 934, 1310, 2656, 3622
AvaI CYCGRG 1 cut(s) 2353
AvaII GGWCC 5 cut(s) 1357, 2122, 2569, 4766, 5231
AxyI CCTNAGG 1 cut(s) 3987
BaeGI GKGCMC 3 cut(s) 561, 1281, 4664
BaeI ACNNNNGTAYC 2 cut(s) 4428, 4461
BanI GGYRCC 2 cut(s) 3623, 4552
BanII GRGCYC 2 cut(s) 168, 3652
BarI GAAGNNNNNNTAC 2 cut(s) 3684, 3716
BauI CACGAG 1 cut(s) 789
BbsI GAAGAC 9 cut(s) 189, 2468, 2813, 3027, 3345, 3827, 3855, 4578, 4609
Bbv12I GWGCWC 6 cut(s) 168, 262, 631, 2991, 3652, 4664
BceAI ACGGC 3 cut(s) 321, 577, 4768
BcgI CGANNNNNNTGC 2 cut(s) 4804, 4838
BciT130I CCWGG 6 cut(s) 304, 476, 882, 1361, 1721, 3874
BclI TGATCA 1 cut(s) 4482
BcnI CCSGG 1 cut(s) 650
BfaI CTAG 9 cut(s) 1254, 1478, 2745, 3149, 3360, 3611, 3731, 5033, 5219
BfmI CTRYAG 6 cut(s) 126, 519, 1683, 3099, 3443, 4266
BfoI RGCGCY 1 cut(s) 3389
BfrI CTTAAG 1 cut(s) 1634
BfuAI ACCTGC 4 cut(s) 1207, 3801, 4456, 5261
BglII AGATCT 1 cut(s) 4641
BlpI GCTNAGC 2 cut(s) 1739, 3696
BmcAI AGTACT 1 cut(s) 3211
Bme1390I CCNGG 7 cut(s) 304, 476, 650, 882, 1361, 1721, 3874
Bme18I GGWCC 5 cut(s) 1357, 2122, 2569, 4766, 5231
BmeT110I CYCGRG 1 cut(s) 2353
BmgBI CACGTC 2 cut(s) 1328, 3571
BmgT120I GGNCC 8 cut(s) 1357, 1717, 2122, 2145, 2569, 4329, 4766, 5231
BmiI GGNNCC 5 cut(s) 1358, 2124, 3625, 4554, 5233
BmrFI CCNGG 7 cut(s) 304, 476, 650, 882, 1361, 1721, 3874
BmrI ACTGGG 1 cut(s) 258
BmsI GCATC 9 cut(s) 1527, 1582, 2348, 2696, 3408, 3697, 3889, 4658, 5170
BmuI ACTGGG 1 cut(s) 258
BpiI GAAGAC 9 cut(s) 189, 2468, 2813, 3027, 3345, 3827, 3855, 4578, 4609
BplI GAGNNNNNCTC 2 cut(s) 1589, 1621
BpmI CTGGAG 6 cut(s) 239, 276, 1742, 1929, 2570, 3114
Bpu10I CCTNAGC 1 cut(s) 4770
Bpu1102I GCTNAGC 2 cut(s) 1739, 3696
BpuEI CTTGAG 2 cut(s) 1682, 4820
BpuMI CCSGG 1 cut(s) 650
Bsa29I ATCGAT 1 cut(s) 4814
BsaAI YACGTR 1 cut(s) 695
BsaBI GATNNNNATC 1 cut(s) 5184
BsaHI GRCGYC 1 cut(s) 361
BsaI GGTCTC 2 cut(s) 1613, 4412
BsaJI CCNNGG 5 cut(s) 303, 881, 2815, 3131, 3873
BsaXI ACNNNNNCTCC 4 cut(s) 2783, 2813, 3282, 3312
Bsc4I CCNNNNNNNGG 7 cut(s) 351, 2132, 2834, 2998, 3133, 3779, 4467
Bse118I RCCGGY 1 cut(s) 262
Bse21I CCTNAGG 1 cut(s) 3987
Bse3DI GCAATG 5 cut(s) 445, 1141, 3725, 3798, 4503
Bse8I GATNNNNATC 1 cut(s) 5184
BseBI CCWGG 6 cut(s) 304, 476, 882, 1361, 1721, 3874
BseCI ATCGAT 1 cut(s) 4814
BseDI CCNNGG 5 cut(s) 303, 881, 2815, 3131, 3873
BseJI GATNNNNATC 1 cut(s) 5184
BseLI CCNNNNNNNGG 7 cut(s) 351, 2132, 2834, 2998, 3133, 3779, 4467
BseMI GCAATG 5 cut(s) 445, 1141, 3725, 3798, 4503
BseRI GAGGAG 1 cut(s) 878
BseSI GKGCMC 3 cut(s) 561, 1281, 4664
BseYI CCCAGC 1 cut(s) 11
BsgI GTGCAG 2 cut(s) 629, 5040
Bsh1236I CGCG 1 cut(s) 1062
Bsh1285I CGRYCG 1 cut(s) 3761
BshFI GGCC 6 cut(s) 336, 1664, 1719, 2147, 2366, 4331
BshNI GGYRCC 2 cut(s) 3623, 4552
BshVI ATCGAT 1 cut(s) 4814
BsiEI CGRYCG 1 cut(s) 3761
BsiHKAI GWGCWC 6 cut(s) 168, 262, 631, 2991, 3652, 4664
BsiHKCI CYCGRG 1 cut(s) 2353
BsiSI CCGG 2 cut(s) 263, 650
BslI CCNNNNNNNGG 7 cut(s) 351, 2132, 2834, 2998, 3133, 3779, 4467
BsmBI CGTCTC 2 cut(s) 352, 975
BsmI GAATGC 8 cut(s) 181, 1135, 2915, 3286, 3855, 3882, 3884, 4499
BsnI GGCC 6 cut(s) 336, 1664, 1719, 2147, 2366, 4331
Bso31I GGTCTC 2 cut(s) 1613, 4412
BsoBI CYCGRG 1 cut(s) 2353
Bsp1286I GDGCHC 8 cut(s) 168, 262, 561, 631, 1281, 2991, 3652, 4664
Bsp1407I TGTACA 1 cut(s) 1670
Bsp1720I GCTNAGC 2 cut(s) 1739, 3696
BspACI CCGC 5 cut(s) 682, 1379, 2632, 4942, 5049
BspANI GGCC 6 cut(s) 336, 1664, 1719, 2147, 2366, 4331
BspDI ATCGAT 1 cut(s) 4814
BspFNI CGCG 1 cut(s) 1062
BspHI TCATGA 2 cut(s) 2344, 4186
BspLI GGNNCC 5 cut(s) 1358, 2124, 3625, 4554, 5233
BspMAI CTGCAG 4 cut(s) 130, 523, 3103, 3447
BspMI ACCTGC 4 cut(s) 1207, 3801, 4456, 5261
BspT107I GGYRCC 2 cut(s) 3623, 4552
BspTI CTTAAG 1 cut(s) 1634
BspTNI GGTCTC 2 cut(s) 1613, 4412
BsrDI GCAATG 5 cut(s) 445, 1141, 3725, 3798, 4503
BsrFI RCCGGY 1 cut(s) 262
BsrGI TGTACA 1 cut(s) 1670
BssAI RCCGGY 1 cut(s) 262
BssECI CCNNGG 5 cut(s) 303, 881, 2815, 3131, 3873
BssNAI GTATAC 1 cut(s) 1641
BssNI GRCGYC 1 cut(s) 361
BssSI CACGAG 1 cut(s) 789
BssT1I CCWWGG 1 cut(s) 3131
Bst1107I GTATAC 1 cut(s) 1641
Bst2BI CACGAG 1 cut(s) 789
Bst2UI CCWGG 6 cut(s) 304, 476, 882, 1361, 1721, 3874
Bst6I CTCTTC 8 cut(s) 33, 350, 955, 1415, 1635, 3219, 4008, 4410
BstACI GRCGYC 1 cut(s) 361
BstAFI CTTAAG 1 cut(s) 1634
BstAPI GCANNNNNTGC 2 cut(s) 3451, 4505
BstAUI TGTACA 1 cut(s) 1670
BstBAI YACGTR 1 cut(s) 695
BstC8I GCNNGC 4 cut(s) 1516, 2364, 3103, 4676
BstEII GGTNACC 1 cut(s) 2662
BstENI CCTNNNNNAGG 1 cut(s) 2130
BstFNI CGCG 1 cut(s) 1062
BstH2I RGCGCY 1 cut(s) 3389
BstHHI GCGC 4 cut(s) 3388, 4883, 5205, 5268
BstMCI CGRYCG 1 cut(s) 3761
BstNI CCWGG 6 cut(s) 304, 476, 882, 1361, 1721, 3874
BstNSI RCATGY 3 cut(s) 2546, 4678, 5063
BstPI GGTNACC 1 cut(s) 2662
BstSCI CCNGG 7 cut(s) 302, 474, 648, 880, 1359, 1719, 3872
BstSFI CTRYAG 6 cut(s) 126, 519, 1683, 3099, 3443, 4266
BstSLI GKGCMC 3 cut(s) 561, 1281, 4664
BstUI CGCG 1 cut(s) 1062
BstV2I GAAGAC 9 cut(s) 189, 2468, 2813, 3027, 3345, 3827, 3855, 4578, 4609
BstX2I RGATCY 6 cut(s) 2639, 2837, 3089, 3367, 3982, 4641
BstXI CCANNNNNNTGG 4 cut(s) 482, 1075, 3382, 5017
BstYI RGATCY 6 cut(s) 2639, 2837, 3089, 3367, 3982, 4641
BstZ17I GTATAC 1 cut(s) 1641
Bsu15I ATCGAT 1 cut(s) 4814
Bsu36I CCTNAGG 1 cut(s) 3987
BsuRI GGCC 6 cut(s) 336, 1664, 1719, 2147, 2366, 4331
BsuTUI ATCGAT 1 cut(s) 4814
BtrI CACGTC 2 cut(s) 1328, 3571
BtsI GCAGTG 1 cut(s) 3452
BveI ACCTGC 4 cut(s) 1207, 3801, 4456, 5261
Cac8I GCNNGC 4 cut(s) 1516, 2364, 3103, 4676
CaiI CAGNNNCTG 1 cut(s) 3451
CciI TCATGA 2 cut(s) 2344, 4186
CfoI GCGC 4 cut(s) 3388, 4883, 5205, 5268
Cfr10I RCCGGY 1 cut(s) 262
Cfr13I GGNCC 8 cut(s) 1357, 1717, 2122, 2145, 2569, 4329, 4766, 5231
ClaI ATCGAT 1 cut(s) 4814
CseI GACGC 1 cut(s) 2818
CsiI ACCWGGT 2 cut(s) 474, 1359
DraI TTTAAA 1 cut(s) 3340
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 8 cut(s) 33, 350, 955, 1415, 1635, 3219, 4008, 4410
EarI CTCTTC 8 cut(s) 33, 350, 955, 1415, 1635, 3219, 4008, 4410
Ecl136II GAGCTC 2 cut(s) 166, 3650
Eco130I CCWWGG 1 cut(s) 3131
Eco147I AGGCCT 1 cut(s) 2366
Eco24I GRGCYC 2 cut(s) 168, 3652
Eco31I GGTCTC 2 cut(s) 1613, 4412
Eco32I GATATC 1 cut(s) 446
Eco47I GGWCC 5 cut(s) 1357, 2122, 2569, 4766, 5231
Eco53kI GAGCTC 2 cut(s) 166, 3650
Eco57I CTGAAG 4 cut(s) 201, 2825, 3812, 4931
Eco81I CCTNAGG 1 cut(s) 3987
Eco88I CYCGRG 1 cut(s) 2353
Eco91I GGTNACC 1 cut(s) 2662
EcoICRI GAGCTC 2 cut(s) 166, 3650
EcoNI CCTNNNNNAGG 1 cut(s) 2130
EcoO109I RGGNCCY 2 cut(s) 1357, 5231
EcoO65I GGTNACC 1 cut(s) 2662
EcoRI GAATTC 3 cut(s) 1098, 2407, 4991
EcoRII CCWGG 6 cut(s) 302, 474, 880, 1359, 1719, 3872
EcoRV GATATC 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 3131
EcoT22I ATGCAT 5 cut(s) 1135, 3286, 3884, 5065, 5185
EcoT38I GRGCYC 2 cut(s) 168, 3652
ErhI CCWWGG 1 cut(s) 3131
Esp3I CGTCTC 2 cut(s) 352, 975
FauI CCCGC 1 cut(s) 5056
FauNDI CATATG 3 cut(s) 1489, 1930, 2974
FbaI TGATCA 1 cut(s) 4482
FblI GTMKAC 3 cut(s) 1557, 1640, 3670
FriOI GRGCYC 2 cut(s) 168, 3652
FspBI CTAG 9 cut(s) 1254, 1478, 2745, 3149, 3360, 3611, 3731, 5033, 5219
FspI TGCGCA 1 cut(s) 4882
GlaI GCGC 4 cut(s) 3387, 4882, 5204, 5267
GsaI CCCAGC 1 cut(s) 15
GsuI CTGGAG 6 cut(s) 239, 276, 1742, 1929, 2570, 3114
HaeII RGCGCY 1 cut(s) 3389
HaeIII GGCC 6 cut(s) 336, 1664, 1719, 2147, 2366, 4331
HapII CCGG 2 cut(s) 263, 650
HgaI GACGC 1 cut(s) 2818
HhaI GCGC 4 cut(s) 3388, 4883, 5205, 5268
Hin1I GRCGYC 1 cut(s) 361
Hin6I GCGC 4 cut(s) 3386, 4881, 5203, 5266
HinP1I GCGC 4 cut(s) 3386, 4881, 5203, 5266
HincII GTYRAC 1 cut(s) 4393
HindII GTYRAC 1 cut(s) 4393
HindIII AAGCTT 1 cut(s) 4540
HpaII CCGG 2 cut(s) 263, 650
HphI GGTGA 5 cut(s) 95, 934, 1310, 2656, 3622
Hpy99I CGWCG 2 cut(s) 1329, 1848
HpyCH4IV ACGT 8 cut(s) 361, 694, 968, 1327, 1846, 3012, 3394, 3570
HpySE526I ACGT 8 cut(s) 361, 694, 968, 1327, 1846, 3012, 3394, 3570
Hsp92I GRCGYC 1 cut(s) 361
HspAI GCGC 4 cut(s) 3386, 4881, 5203, 5266
Ksp22I TGATCA 1 cut(s) 4482
LmnI GCTCC 9 cut(s) 163, 257, 640, 1723, 2136, 2817, 2994, 3095, 3647
LweI GCATC 9 cut(s) 1527, 1582, 2348, 2696, 3408, 3697, 3889, 4658, 5170
MabI ACCWGGT 2 cut(s) 474, 1359
MaeI CTAG 9 cut(s) 1254, 1478, 2745, 3149, 3360, 3611, 3731, 5033, 5219
MaeII ACGT 8 cut(s) 361, 694, 968, 1327, 1846, 3012, 3394, 3570
MfeI CAATTG 4 cut(s) 1888, 2073, 3533, 3956
MflI RGATCY 6 cut(s) 2639, 2837, 3089, 3367, 3982, 4641
MhlI GDGCHC 8 cut(s) 168, 262, 561, 631, 1281, 2991, 3652, 4664
MlyI GAGTC 7 cut(s) 208, 311, 1250, 1598, 1666, 3487, 4093
MmeI TCCRAC 2 cut(s) 618, 3876
Mph1103I ATGCAT 5 cut(s) 1135, 3286, 3884, 5065, 5185
MroXI GAANNNNTTC 3 cut(s) 2465, 3174, 3882
MslI CAYNNNNRTG 2 cut(s) 3380, 4156
MspA1I CMGCKG 1 cut(s) 5054
MspCI CTTAAG 1 cut(s) 1634
MspI CCGG 2 cut(s) 263, 650
MspR9I CCNGG 7 cut(s) 304, 476, 650, 882, 1361, 1721, 3874
MunI CAATTG 4 cut(s) 1888, 2073, 3533, 3956
Mva1269I GAATGC 8 cut(s) 181, 1135, 2915, 3286, 3855, 3882, 3884, 4499
MvaI CCWGG 6 cut(s) 304, 476, 882, 1361, 1721, 3874
MvnI CGCG 1 cut(s) 1062
NciI CCSGG 1 cut(s) 650
NdeI CATATG 3 cut(s) 1489, 1930, 2974
NlaIV GGNNCC 5 cut(s) 1358, 2124, 3625, 4554, 5233
NmeAIII GCCGAG 1 cut(s) 3457
NmuCI GTSAC 7 cut(s) 112, 1009, 2662, 2968, 3677, 4023, 4351
NsbI TGCGCA 1 cut(s) 4882
NsiI ATGCAT 5 cut(s) 1135, 3286, 3884, 5065, 5185
NspI RCATGY 3 cut(s) 2546, 4678, 5063
PaeI GCATGC 1 cut(s) 4678
PaeR7I CTCGAG 1 cut(s) 2353
PagI TCATGA 2 cut(s) 2344, 4186
PaqCI CACCTGC 1 cut(s) 3801
PceI AGGCCT 1 cut(s) 2366
PctI GAATGC 8 cut(s) 181, 1135, 2915, 3286, 3855, 3882, 3884, 4499
PdmI GAANNNNTTC 3 cut(s) 2465, 3174, 3882
PflMI CCANNNNNTGG 1 cut(s) 2998
Ple19I CGATCG 1 cut(s) 3761
PleI GAGTC 7 cut(s) 208, 311, 1249, 1598, 1665, 3487, 4092
PpsI GAGTC 7 cut(s) 208, 311, 1249, 1598, 1665, 3487, 4092
Ppu21I YACGTR 1 cut(s) 695
PpuMI RGGWCCY 2 cut(s) 1357, 5231
PshBI ATTAAT 1 cut(s) 536
PsiI TTATAA 2 cut(s) 2478, 3837
Psp124BI GAGCTC 2 cut(s) 168, 3652
Psp5II RGGWCCY 2 cut(s) 1357, 5231
Psp6I CCWGG 6 cut(s) 302, 474, 880, 1359, 1719, 3872
PspEI GGTNACC 1 cut(s) 2662
PspFI CCCAGC 1 cut(s) 11
PspGI CCWGG 6 cut(s) 302, 474, 880, 1359, 1719, 3872
PspN4I GGNNCC 5 cut(s) 1358, 2124, 3625, 4554, 5233
PspPI GGNCC 8 cut(s) 1357, 1717, 2122, 2145, 2569, 4329, 4766, 5231
PspPPI RGGWCCY 2 cut(s) 1357, 5231
PsrI GAACNNNNNNTAC 2 cut(s) 525, 557
PstI CTGCAG 4 cut(s) 130, 523, 3103, 3447
PstNI CAGNNNCTG 1 cut(s) 3451
PsuI RGATCY 6 cut(s) 2639, 2837, 3089, 3367, 3982, 4641
PvuI CGATCG 1 cut(s) 3761
PvuII CAGCTG 1 cut(s) 5054
RseI CAYNNNNRTG 2 cut(s) 3380, 4156
SacI GAGCTC 2 cut(s) 168, 3652
Sau96I GGNCC 8 cut(s) 1357, 1717, 2122, 2145, 2569, 4329, 4766, 5231
ScaI AGTACT 1 cut(s) 3211
SchI GAGTC 7 cut(s) 208, 311, 1250, 1598, 1666, 3487, 4093
ScrFI CCNGG 7 cut(s) 304, 476, 650, 882, 1361, 1721, 3874
SduI GDGCHC 8 cut(s) 168, 262, 561, 631, 1281, 2991, 3652, 4664
SexAI ACCWGGT 2 cut(s) 474, 1359
SfaNI GCATC 9 cut(s) 1527, 1582, 2348, 2696, 3408, 3697, 3889, 4658, 5170
SfcI CTRYAG 6 cut(s) 126, 519, 1683, 3099, 3443, 4266
Sfr274I CTCGAG 1 cut(s) 2353
SinI GGWCC 5 cut(s) 1357, 2122, 2569, 4766, 5231
SlaI CTCGAG 1 cut(s) 2353
SmiMI CAYNNNNRTG 2 cut(s) 3380, 4156
SmlI CTYRAG 4 cut(s) 1634, 1697, 2353, 4799
SmoI CTYRAG 4 cut(s) 1634, 1697, 2353, 4799
SphI GCATGC 1 cut(s) 4678
SseBI AGGCCT 1 cut(s) 2366
SsiI CCGC 5 cut(s) 682, 1379, 2632, 4942, 5049
SspI AATATT 1 cut(s) 2240
SspMI CTAG 9 cut(s) 1254, 1478, 2745, 3149, 3360, 3611, 3731, 5033, 5219
SstI GAGCTC 2 cut(s) 168, 3652
StuI AGGCCT 1 cut(s) 2366
StyD4I CCNGG 7 cut(s) 302, 474, 648, 880, 1359, 1719, 3872
StyI CCWWGG 1 cut(s) 3131
TaiI ACGT 8 cut(s) 364, 697, 971, 1330, 1849, 3015, 3397, 3573
TaqI TCGA 5 cut(s) 1324, 1705, 2354, 4704, 4814
TaqII GACCGA 1 cut(s) 248
TatI WGTACW 6 cut(s) 192, 1670, 3209, 3573, 4180, 4886
TauI GCSGC 3 cut(s) 685, 2635, 4945
TseFI GTSAC 7 cut(s) 112, 1009, 2662, 2968, 3677, 4023, 4351
Tsp45I GTSAC 7 cut(s) 112, 1009, 2662, 2968, 3677, 4023, 4351
TspGWI ACGGA 3 cut(s) 1351, 2309, 2850
Van91I CCANNNNNTGG 1 cut(s) 2998
Vha464I CTTAAG 1 cut(s) 1634
VneI GTGCAC 1 cut(s) 4660
VpaK11BI GGWCC 5 cut(s) 1357, 2122, 2569, 4766, 5231
VspI ATTAAT 1 cut(s) 536
XagI CCTNNNNNAGG 1 cut(s) 2130
XapI RAATTY 8 cut(s) 455, 1098, 1349, 2407, 2576, 2618, 4614, 4991
XbaI TCTAGA 1 cut(s) 2744
XceI RCATGY 3 cut(s) 2546, 4678, 5063
XcmI CCANNNNNNNNNTGG 1 cut(s) 4747
XhoI CTCGAG 1 cut(s) 2353
XmiI GTMKAC 3 cut(s) 1557, 1640, 3670
XmnI GAANNNNTTC 3 cut(s) 2465, 3174, 3882
XspI CTAG 9 cut(s) 1254, 1478, 2745, 3149, 3360, 3611, 3731, 5033, 5219
ZraI GACGTC 1 cut(s) 362
ZrmI AGTACT 1 cut(s) 3211
Zsp2I ATGCAT 5 cut(s) 1135, 3286, 3884, 5065, 5185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.