MD15G1063200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003946
Physical Location & Seq
Reverse (-)
4344894 .. 4350915
6022 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1063200.v1.1.491

Sequence Viewer

Length: 5187 bp
ATGGCAAACGACGCAAATGAAGCTTTACGTTCCGCTAAACTTCACATAGAAGAGATTAGGACCAAAAAGTTCTCAATAGGGAAGAAAGAAGCAAACCCTTTGACTCTCGATCTTCACCATGCCGTCACTAGTCTCTCTGCCGAGCTTTACCAGAAGGACATCCATTTTCTCATGGAGCTCATACAGAATGCAGAAGACAATGAATACAAAGAAGGGGTGGAACCAACATTGGAATTTGTGCTGACAAAGAAGGACATAACTGGGAGTGGAGCACCAGCTACTCTGCTTGTTTTTAATAACGAGGTTGGCTTTTCCAGGAAGAATATGGACTCGATTTGCAGCATAGGCCGTTCCACAAAGAAGGGAAAGAGACGGCAGGGATTTATTGGAGAAAAGGGTATTGGATTTAAAAGTGTATTCCTTGTGAGTTCACGGCCTCATATATTTAGCAACGGATATCAGGTCAGTTTTATGGAAGAACCAAATCAAGATTGTGGTATTGGTTATATAGTTCCTGAATGGGTTAGTGGAAAGCCAAATTTATCAAGTATATGGGAGGTGTATGGTTTCAACAAAATCTTGCCAACAACTACGTTTATTCTTCCTCTAAAGCCTGACAAGGTAGAAGCTGTGAGAGTGCAGCTGTCAGAGCTGCACCCAGAGCTTCTCCTGTTCTTATCCAAGATAAAGCGCATATACGTACGTGGATGTGATCCACAAGAAGCTGATGATGTCTCCACAATCTCTATATTCAGTGAGACTGAGCATGTGAACTTGAGTGATAAAGGAGCCAATTCACTTGTTGTTCAACTTTCTGCGAAAGAAAAAAAATGTGATACTGATGAGTTGTGCAAGTATTATTTGTGGAGAGAGTCATTTCCAGTAAAACCGGGCAATAGAGTTAATACAAGGATGGATGTGGAGGAATGGGTCATCACCCTTGCTTTCCCATTTGGAGAAAGACTGAGAAGGGGAACGTCGTCTGTTGGTGTATTTTCTTTCCTGCCGACTGCAATGGTCACCAACCTCCCCTTTGTAATTCAAGCTGATTTCATTCTTGCTTCTTCACGAGAATCTATTCTGTTGGATGATGTCTGGAACTTGGGAATACTTGAATGTGTGCCATCCGCCTTTGTGAATGCCTTCCAGTCATGTGTGAGAGAGCTCTCCCTCTTTCCTTCACCTGGTCAGGCATTTGAGTTCTTACCTGCTCAAGCTTCTCCGATTCCCACATTTGACGACTTAAGGGAGTCTATTAGAATTAAATTGGAAGGTTTACAGATAGTGCCTTGTCAAATGCTTTCTGGCAGGAATTGTTTGTTCCTGCAGCCTAAACATGCAGTTCGGATCTTGCCAAAATTTAGGGCCCTAGTGCTTCAAATGAAAAGTGAAGGGGCTTTTTCAAGTGGTTCACCTTTATTGAAGAGGGTCTTGCATTCGTCTCTGGACCTTGAAAAATACAGTGCAGTTCTGTACTTTCTAGGTGTGGTATGGGCTAGTGGTCATTGGTACACAGAATGCATCAGATCCTGCAATCTTCTGATGGTATCTGATGATGTCTACATTGATCTGCTTGATTTTATTGCTGACAATGAGGTAATGACGTCAAAGCATATTTCGGTCATACCTCTTATTAAATACATCAATCGGGAAGGCAATTTGCAATTGTGTACAATTACCCAAACCGCAAAGGCGGAGTCCAAGATTCAGTATGCTGAGGAGACGGAAGTTCATACATGGCTGAACAAATGCAATATGGAGTTTGGATGTCCTGATAATATGTATTTCTTGCCAGATAATGTACAAAAAGCTCTTTTAGATAATCAGAGAAGTGTTGCCAGAGAGAAAAAATCAAGGCACGGGATAAAATCTTCTGTCTGCAATTGGCTTTGCAACTATCCAAGAGTGAAATCTTGTACAGCATATGATTATGCTGTATTTCTTTGCAATCATGTAAACAAGGAGCCAGGTCTTGCACTTACCCTTGCTAATTTCCTTTATCATGCGCACAAGAAATCCTTCCTTAATGATTATGAGACTTCTTATATTTGTAGAAGTTTCCCGCTTGTTGATGGAGCTGACCGTGTCAGAATGCAAAGAACTGTTACTCTTGTCCCTGCATCAGGTAGCAAATGGGTGAAGTTGTTTGGTTCCCAGAATCCATTTGTAGAACAGAACTATGTTGATATAGGGAATGCTTACGCAAAAAGTAGTATGTTTTTGGGAGACTCGGTGCTTGAGAAGGAGCTTCTTAATTTTATTGAAAAGCACTGTAAAGCTATGGACTTACCCGAGTTGTCTCCTCCAGATGTGGTTTTAGAGAAAGCATCTCATGAACTATCTAGTGAGCAGGCCTTTTTGCTGCTTGATTGGATCAGATTACTCCGGACTAAGGGTTCATCTCTTCCGACAAAGTTCATTGAAAGCATTCGAGGTGGTAAGTGGATGAAGACATACTCAGGTTATAGTTCCCCAAGAAAGGCCATTCTTCCAGATGAAGCAGGTAAATCTATTCTTGATATGATGAAGCATGTTCTGAAGGATGTCTGTATTATAGACCTGGAATTTTACATGAATCGGATCAATCTTTATCAAGATGAATTGAAATTTCTTGGTGTCGGGCATGGATCATATGATGTGAGGAAGCTGGTTACTAATTGCTTAAAGTCTCTTGCTTCTCCTCAAATGAGCAAAGACTGCGCATTTTCTTTGTTGAATTTCATTAGTTTCTGTAGAGGGAGGAATGTGATTGACAAGGATTGGTTGACTGTTATGAAGGAGAAGAAGTGGCTGAAGACTCATCAGGGTTATAGTGCTCCAAAGGGAACTATTCTTTTGCCGTCTGAGATAGAAGTGGAAACCTGTTTGAAAATCACAAATCTTCCAATTGTTGATGAAACATTCTATGGAAGCAGATTAAGTTGTTTCATATCTGAGTTAACGCTACTTGGAGTTGCATGTGGTCTTGAGGAGGTGCAGAAATCGATTGCAGAGAATATGAATTTAACATCAAATCTGTCATCCATGACTGGTAGTTGTGGTTTGTTGATTCTTAAGTGTATCAGATGCTTGGGGTCTAGGGCTGCAGGCTTAATTAACAGGATTAAATGTCAACCTTGGATGAAGACAAATTTGGGTTTTAAAAGCCCTTCAGAAACGGCTCTTCCTGGTTCCCAATGGGGTTCTTTGTTCACTGCTCTCCAGGTTCCTGCCATAGATGCACAGTATTATGGGGATGCAATTACACATTTTGTTGAGGAGTTGAATGCCTTGGAAGTTGTAATGTCACTGCTAGGTTGCATCCGGGAACTGAGTCAAACTATGTCTTTATCGTCCCCTGAACTGATGTGGTTGACATCTGAGAAGTGGTTAAAAACACGACATGGTTACCAGACTCCTAGAGAATCAATTCTTTTTAGCTCCAAATGGGGGTCAATCTCGTTCTTTGTTGATCTTCCTCTAATTGATGACGCGTATTATGGTATCGACATATATAAGTTCAGGGATGAGCTGCAGATATTGGGTGTAATCATGGATTTTGAACAAGGAGCATCATTCGTTGCTAAAGGTGTCCGCAGCCCTATTGAAGGGGAACTACTTAGTGCAGATGGTATGATGTCAATTCTAGAGTGCATTAAGCATCTGATGTCAAGTTCTCTTGATGAGGCTTTAGTGGACGACTTGGTCAGTAATGTCGCAAAAGGCAGGTGTTTGAAGACCGTGAGTGGTTACAAAACTCCAGAAGAATGTGTTCTTTTTGATCCAACATGGGATTGTATTCTAAAGAGGTCGGATGTGCCCAGCATTGATGAATTTTATTTTGGAACTAACATTCACGCATATAAGAATCAGTTGAGAGATATTGGTGTGAAGGTAGATCTTTTGGATGTTTGTTCTTTTCTTTCTGCATTTCTTTTGTCCCAGACAAAAACGGCTGTAATAAGAAGGATTTATAGCTTCCTTAACAAGTTCAAGTGGAGTCCAACAGTTCAAGATAAAAGCAACTCTCAGGTGTGGATACCTAATCCCGAGGGCCCAGGTGTGTGGGTTAATTCTCAAGATTGTGTACTTCATGATCGGAAAAAGCTATTCGGCTCCAGCCTGTTTTGTCTTGACAAGTTTTACAAGAAAGAGCTCCTCCCCGTGTTTTCTTCAGCTTTTGGAGTTTCCGATAATCCTTCCGTCAGTAACTACGTGCAGCTATGGAACAATTGGGCCTTGAGGGATAACTCTCAAGTAACTGTCAGAGAATGCAGCTTGTTTTGCGAATATATTGTAGACAACTGGAATCAAGACATAGAAGATAATCTGAAGCTGAACTTGATCAGATTACCTGCCACAATGTCCACAGTTGAAGAAATATATCTGATGAGTAGAGAGGAAGTGTTCATTGCTGATGACTTGCAACTGAAAAAGATTTTCTCGAGCTTCGACAAGGCTCCTTTGTTTGTGTGGATTCCCAACAGTTTCAGCGAGTTTCTTATTTCTCCTAGAAGGCTGCATCAAATTTATGAATTACTTGGAGTTAGAAAGGTTTCTGACTCAGTTGAGTGCAATGTCAGTAGCATGCTATCGGTGGATCATTGTGAAAAGGTTGATGCAAGAAACGGATTGATAGGAAGGGGCTTAATCAAAATTATTCTCGGTTTTCTTGCTGGTCCCGAGGTAAACATGTCAATGAAAGAGAGACACAACGCTGCTAAATCAGTGGTTATGCTGTCGGTGTACAAGAGCGACAAACCAATTCAAGTCTGTTACAGACTAGTTCCATCTGCAAGTACATCAGTGGAAGTGGAAAAGTTAAAGCTGGTTCTTTGGGAGAAGGATTCTCAGCGGTTGCTTATGGATAAATCTGGCTATGAAAACGGGAAAGATGATCTTGAATTTGTTACGTCTTTTGCTGACGAACTCGCACAAGGATTGTTAGCACAGGCAAAACCCACTGCAGCAGATACATTGAGTAAGATTATCCAAATTGGGTTCATGTTTCATTTCAATGAGAATGAAGTTGATTTTTTGCTGATGAAGCAAAATTTGGAGCTGTTAGTGGAGGATGTGGCGTTTCTCGACAGTGCGTTCCTCTCTTCAGAAGGACCTTCTCGTGTTTACCGAAAGCGTGCAAGCAAGAAGTTGGAGCTTGTAGGCCCTTCCACACCAATGCTTTCCGGCAAGAAACGACGTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1729

Amino Acids

195.0

Weight (kDa)

6.43

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 50 - 155 4e-10 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3696
AasI GACNNNNNNGTC 1 cut(s) 3683
AatII GACGTC 2 cut(s) 1607, 5182
Acc16I TGCGCA 2 cut(s) 2007, 2701
Acc36I ACCTGC 4 cut(s) 1216, 2492, 3696, 4342
AccI GTMKAC 2 cut(s) 1560, 4278
AccII CGCG 1 cut(s) 3473
AccIII TCCGGA 1 cut(s) 2385
AciI CCGC 7 cut(s) 33, 1128, 1686, 1694, 2063, 3574, 4834
AclWI GGATC 8 cut(s) 707, 1355, 1521, 2381, 2588, 2635, 3755, 4587
AcuI CTGAAG 6 cut(s) 2558, 2813, 3135, 4137, 4331, 5070
AcyI GRCGYC 2 cut(s) 1604, 5179
AfaI GTAC 9 cut(s) 702, 1475, 1511, 1672, 1803, 1918, 4068, 4727, 4780
AfiI CCNNNNNNNGG 6 cut(s) 1184, 2123, 2392, 2479, 3429, 3587
AflII CTTAAG 2 cut(s) 1243, 3053
AflIII ACRYGT 2 cut(s) 3471, 4671
AhlI ACTAGT 2 cut(s) 128, 4762
AjnI CCWGG 7 cut(s) 314, 1183, 1966, 2559, 3166, 3201, 4036
AjuI GAANNNNNNNTTGG 8 cut(s) 384, 416, 3116, 3148, 3804, 3836, 5018, 5050
AloI GAACNNNNNNTCC 2 cut(s) 2380, 2412
Alw21I GWGCWC 5 cut(s) 180, 274, 1167, 2818, 4137
AlwI GGATC 8 cut(s) 707, 1355, 1521, 2381, 2588, 2635, 3755, 4587
AlwNI CAGNNNCTG 1 cut(s) 1530
Ama87I CYCGRG 4 cut(s) 2291, 4028, 4423, 4661
Aor13HI TCCGGA 1 cut(s) 2385
AoxI GGCC 8 cut(s) 346, 434, 1365, 2352, 2481, 4033, 4215, 5143
ApaI GGGCCC 2 cut(s) 1369, 4037
ArsI GACNNNNNNTTYG 2 cut(s) 1682, 1714
Asp700I GAANNNNTTC 7 cut(s) 1077, 1142, 2018, 2427, 3408, 3750, 4534
AspLEI GCGC 3 cut(s) 693, 2008, 2702
AsuC2I CCSGG 2 cut(s) 891, 3305
AsuHPI GGTGA 6 cut(s) 107, 928, 1012, 1173, 1404, 2149
AvaI CYCGRG 4 cut(s) 2291, 4028, 4423, 4661
AvaII GGWCC 4 cut(s) 60, 1447, 4658, 5093
BaeGI GKGCMC 3 cut(s) 1369, 3801, 4037
BanII GRGCYC 5 cut(s) 180, 1167, 1369, 4037, 4137
BarI GAAGNNNNNNTAC 2 cut(s) 3579, 3611
BauI CACGAG 2 cut(s) 1068, 5100
BbsI GAAGAC 5 cut(s) 201, 2456, 2801, 3131, 3722
Bbv12I GWGCWC 5 cut(s) 180, 274, 1167, 2818, 4137
BbvCI CCTCAGC 1 cut(s) 1716
BccI CCATC 6 cut(s) 907, 1132, 1537, 2066, 3602, 4777
BceAI ACGGC 7 cut(s) 107, 333, 389, 449, 2824, 3174, 3948
BciT130I CCWGG 7 cut(s) 316, 1185, 1968, 2561, 3168, 3203, 4038
BciVI GTATCC 1 cut(s) 4011
BclI TGATCA 1 cut(s) 4323
BcnI CCSGG 2 cut(s) 891, 3305
BcuI ACTAGT 2 cut(s) 128, 4762
BfmI CTRYAG 5 cut(s) 1325, 2731, 3084, 3512, 4944
BfrI CTTAAG 2 cut(s) 1243, 3053
BfuAI ACCTGC 4 cut(s) 1216, 2492, 3696, 4342
BfuI GTATCC 1 cut(s) 4011
BglII AGATCT 1 cut(s) 3877
Bme1390I CCNGG 9 cut(s) 316, 891, 1185, 1968, 2561, 3168, 3203, 3305, 4038
Bme18I GGWCC 4 cut(s) 60, 1447, 4658, 5093
BmeT110I CYCGRG 4 cut(s) 2291, 4028, 4423, 4661
BmrFI CCNGG 9 cut(s) 316, 891, 1185, 1968, 2561, 3168, 3203, 3305, 4038
BmrI ACTGGG 1 cut(s) 270
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 5 cut(s) 201, 2456, 2801, 3131, 3722
BpmI CTGGAG 4 cut(s) 2289, 3185, 3723, 4081
Bpu10I CCTNAGC 1 cut(s) 1716
BpuEI CTTGAG 7 cut(s) 796, 1197, 2258, 2987, 4041, 4218, 4240
BpuMI CCSGG 2 cut(s) 891, 3305
Bsa29I ATCGAT 1 cut(s) 2984
BsaAI YACGTR 3 cut(s) 700, 704, 4195
BsaHI GRCGYC 2 cut(s) 1604, 5179
BsaJI CCNNGG 5 cut(s) 3116, 3270, 4029, 4036, 4662
BsaWI WCCGGW 1 cut(s) 2385
BsaXI ACNNNNNCTCC 4 cut(s) 2217, 2247, 2771, 2801
Bsc4I CCNNNNNNNGG 6 cut(s) 1184, 2123, 2392, 2479, 3429, 3587
Bse1I ACTGG 5 cut(s) 265, 881, 1147, 3034, 4289
Bse3DI GCAATG 3 cut(s) 1020, 4389, 4561
BseAI TCCGGA 1 cut(s) 2385
BseBI CCWGG 7 cut(s) 316, 1185, 1968, 2561, 3168, 3203, 4038
BseCI ATCGAT 1 cut(s) 2984
BseDI CCNNGG 5 cut(s) 3116, 3270, 4029, 4036, 4662
BseLI CCNNNNNNNGG 6 cut(s) 1184, 2123, 2392, 2479, 3429, 3587
BseMI GCAATG 3 cut(s) 1020, 4389, 4561
BseNI ACTGG 5 cut(s) 265, 881, 1147, 3034, 4289
BseRI GAGGAG 6 cut(s) 1733, 2292, 2670, 2984, 3272, 4127
BseSI GKGCMC 3 cut(s) 1369, 3801, 4037
BseYI CCCAGC 1 cut(s) 3800
BsgI GTGCAG 6 cut(s) 638, 659, 1485, 2996, 3624, 4217
Bsh1236I CGCG 1 cut(s) 3473
BshFI GGCC 8 cut(s) 348, 436, 1367, 2354, 2483, 4035, 4217, 5145
BshVI ATCGAT 1 cut(s) 2984
BsiHKAI GWGCWC 5 cut(s) 180, 274, 1167, 2818, 4137
BsiHKCI CYCGRG 4 cut(s) 2291, 4028, 4423, 4661
BsiSI CCGG 4 cut(s) 890, 2386, 3304, 5166
BsiWI CGTACG 1 cut(s) 700
BslFI GGGAC 4 cut(s) 2099, 3319, 3904, 4644
BslI CCNNNNNNNGG 6 cut(s) 1184, 2123, 2392, 2479, 3429, 3587
BsmBI CGTCTC 3 cut(s) 364, 1446, 1715
BsmFI GGGAC 4 cut(s) 2099, 3319, 3904, 4644
BsmI GAATGC 9 cut(s) 193, 1144, 1435, 1523, 2097, 2200, 2427, 3271, 4256
BsnI GGCC 8 cut(s) 348, 436, 1367, 2354, 2483, 4035, 4217, 5145
BsoBI CYCGRG 4 cut(s) 2291, 4028, 4423, 4661
Bsp120I GGGCCC 2 cut(s) 1365, 4033
Bsp1286I GDGCHC 8 cut(s) 180, 274, 1167, 1369, 2818, 3801, 4037, 4137
Bsp13I TCCGGA 1 cut(s) 2385
Bsp1407I TGTACA 4 cut(s) 1670, 1801, 1916, 4725
BspACI CCGC 7 cut(s) 33, 1128, 1686, 1694, 2063, 3574, 4834
BspANI GGCC 8 cut(s) 348, 436, 1367, 2354, 2483, 4035, 4217, 5145
BspDI ATCGAT 1 cut(s) 2984
BspEI TCCGGA 1 cut(s) 2385
BspFNI CGCG 1 cut(s) 3473
BspHI TCATGA 2 cut(s) 2332, 4072
BspMAI CTGCAG 4 cut(s) 1329, 3088, 3516, 4948
BspMI ACCTGC 4 cut(s) 1216, 2492, 3696, 4342
BspPI GGATC 8 cut(s) 707, 1355, 1521, 2381, 2588, 2635, 3755, 4587
BspQI GCTCTTC 1 cut(s) 3168
BspTI CTTAAG 2 cut(s) 1243, 3053
BsrDI GCAATG 3 cut(s) 1020, 4389, 4561
BsrGI TGTACA 4 cut(s) 1670, 1801, 1916, 4725
BsrI ACTGG 5 cut(s) 265, 881, 1147, 3034, 4289
BssECI CCNNGG 5 cut(s) 3116, 3270, 4029, 4036, 4662
BssNI GRCGYC 2 cut(s) 1604, 5179
BssSI CACGAG 2 cut(s) 1068, 5100
BssT1I CCWWGG 2 cut(s) 3116, 3270
Bst2BI CACGAG 2 cut(s) 1068, 5100
Bst2UI CCWGG 7 cut(s) 316, 1185, 1968, 2561, 3168, 3203, 4038
Bst6I CTCTTC 5 cut(s) 45, 1418, 2409, 3168, 5089
BstACI GRCGYC 2 cut(s) 1604, 5179
BstAFI CTTAAG 2 cut(s) 1243, 3053
BstAPI GCANNNNNTGC 1 cut(s) 2697
BstAUI TGTACA 4 cut(s) 1670, 1801, 1916, 4725
BstBAI YACGTR 3 cut(s) 700, 704, 4195
BstC8I GCNNGC 5 cut(s) 2352, 3088, 4568, 5118, 5122
BstEII GGTNACC 2 cut(s) 1018, 3386
BstENI CCTNNNNNAGG 2 cut(s) 2121, 3585
BstFNI CGCG 1 cut(s) 3473
BstHHI GCGC 3 cut(s) 693, 2008, 2702
BstMWI GCNNNNNNNGC 9 cut(s) 11, 20, 345, 649, 661, 2697, 3218, 4263, 5026
BstNI CCWGG 7 cut(s) 316, 1185, 1968, 2561, 3168, 3203, 4038
BstNSI RCATGY 6 cut(s) 770, 1340, 2534, 2961, 4570, 4675
BstPI GGTNACC 2 cut(s) 1018, 3386
BstSCI CCNGG 9 cut(s) 314, 889, 1183, 1966, 2559, 3166, 3201, 3303, 4036
BstSFI CTRYAG 5 cut(s) 1325, 2731, 3084, 3512, 4944
BstSLI GKGCMC 3 cut(s) 1369, 3801, 4037
BstSNI TACGTA 1 cut(s) 700
BstUI CGCG 1 cut(s) 3473
BstV2I GAAGAC 5 cut(s) 201, 2456, 2801, 3131, 3722
BstX2I RGATCY 3 cut(s) 1347, 1526, 3877
BstXI CCANNNNNNTGG 1 cut(s) 4044
BstYI RGATCY 3 cut(s) 1347, 1526, 3877
Bsu15I ATCGAT 1 cut(s) 2984
BsuI GTATCC 1 cut(s) 4011
BsuRI GGCC 8 cut(s) 348, 436, 1367, 2354, 2483, 4035, 4217, 5145
BsuTUI ATCGAT 1 cut(s) 2984
BtsI GCAGTG 3 cut(s) 3192, 3287, 4941
BtsIMutI CAGTG 9 cut(s) 760, 1468, 2269, 3192, 3287, 4713, 4791, 4941, 5077
BveI ACCTGC 4 cut(s) 1216, 2492, 3696, 4342
Cac8I GCNNGC 5 cut(s) 2352, 3088, 4568, 5118, 5122
CaiI CAGNNNCTG 1 cut(s) 1530
CciI TCATGA 2 cut(s) 2332, 4072
CfoI GCGC 3 cut(s) 693, 2008, 2702
ClaI ATCGAT 1 cut(s) 2984
CseI GACGC 2 cut(s) 20, 3479
CsiI ACCWGGT 1 cut(s) 1183
Csp6I GTAC 9 cut(s) 701, 1474, 1510, 1671, 1802, 1917, 4067, 4726, 4779
CviQI GTAC 9 cut(s) 701, 1474, 1510, 1671, 1802, 1917, 4067, 4726, 4779
DraI TTTAAA 2 cut(s) 409, 3142
DrdI GACNNNNNNGTC 1 cut(s) 3683
DseDI GACNNNNNNGTC 1 cut(s) 3683
Eam1104I CTCTTC 5 cut(s) 45, 1418, 2409, 3168, 5089
EarI CTCTTC 5 cut(s) 45, 1418, 2409, 3168, 5089
EciI GGCGGA 2 cut(s) 1117, 1709
Ecl136II GAGCTC 3 cut(s) 178, 1165, 4135
Eco105I TACGTA 1 cut(s) 700
Eco130I CCWWGG 2 cut(s) 3116, 3270
Eco147I AGGCCT 1 cut(s) 2354
Eco24I GRGCYC 5 cut(s) 180, 1167, 1369, 4037, 4137
Eco32I GATATC 1 cut(s) 458
Eco47I GGWCC 4 cut(s) 60, 1447, 4658, 5093
Eco53kI GAGCTC 3 cut(s) 178, 1165, 4135
Eco57I CTGAAG 6 cut(s) 2558, 2813, 3135, 4137, 4331, 5070
Eco88I CYCGRG 4 cut(s) 2291, 4028, 4423, 4661
Eco91I GGTNACC 2 cut(s) 1018, 3386
EcoICRI GAGCTC 3 cut(s) 178, 1165, 4135
EcoNI CCTNNNNNAGG 2 cut(s) 2121, 3585
EcoO109I RGGNCCY 5 cut(s) 1365, 1366, 4033, 5093, 5144
EcoO65I GGTNACC 2 cut(s) 1018, 3386
EcoRII CCWGG 7 cut(s) 314, 1183, 1966, 2559, 3166, 3201, 4036
EcoRV GATATC 1 cut(s) 458
EcoT14I CCWWGG 2 cut(s) 3116, 3270
EcoT22I ATGCAT 1 cut(s) 1523
EcoT38I GRGCYC 5 cut(s) 180, 1167, 1369, 4037, 4137
ErhI CCWWGG 2 cut(s) 3116, 3270
Esp3I CGTCTC 3 cut(s) 364, 1446, 1715
FalI AAGNNNNNCTT 8 cut(s) 1415, 1447, 2003, 2035, 4304, 4336, 4863, 4895
FaqI GGGAC 4 cut(s) 2099, 3319, 3904, 4644
FauI CCCGC 1 cut(s) 2070
FauNDI CATATG 2 cut(s) 1924, 2632
FbaI TGATCA 1 cut(s) 4323
FblI GTMKAC 2 cut(s) 1560, 4278
FriOI GRGCYC 5 cut(s) 180, 1167, 1369, 4037, 4137
FspAI RTGCGCAY 1 cut(s) 2007
FspI TGCGCA 2 cut(s) 2007, 2701
GlaI GCGC 3 cut(s) 692, 2007, 2701
GsaI CCCAGC 1 cut(s) 3804
GsuI CTGGAG 4 cut(s) 2289, 3185, 3723, 4081
HaeIII GGCC 8 cut(s) 348, 436, 1367, 2354, 2483, 4035, 4217, 5145
HapII CCGG 4 cut(s) 890, 2386, 3304, 5166
HgaI GACGC 2 cut(s) 20, 3479
HhaI GCGC 3 cut(s) 693, 2008, 2702
Hin1I GRCGYC 2 cut(s) 1604, 5179
Hin6I GCGC 3 cut(s) 691, 2006, 2700
HinP1I GCGC 3 cut(s) 691, 2006, 2700
HincII GTYRAC 4 cut(s) 2766, 2940, 3113, 3354
HindII GTYRAC 4 cut(s) 2766, 2940, 3113, 3354
HindIII AAGCTT 2 cut(s) 21, 1215
HpaI GTTAAC 1 cut(s) 2940
HpaII CCGG 4 cut(s) 890, 2386, 3304, 5166
HphI GGTGA 6 cut(s) 107, 928, 1012, 1173, 1404, 2149
Hpy99I CGWCG 3 cut(s) 14, 982, 5181
HpyCH4IV ACGT 9 cut(s) 28, 593, 699, 703, 977, 1604, 4194, 4892, 5179
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 20, 345, 649, 661, 2697, 3218, 4263, 5026
HpySE526I ACGT 9 cut(s) 28, 593, 699, 703, 977, 1604, 4194, 4892, 5179
Hsp92I GRCGYC 2 cut(s) 1604, 5179
HspAI GCGC 3 cut(s) 691, 2006, 2700
Kpn2I TCCGGA 1 cut(s) 2385
Ksp22I TGATCA 1 cut(s) 4323
KspAI GTTAAC 1 cut(s) 2940
LguI GCTCTTC 1 cut(s) 3168
MabI ACCWGGT 1 cut(s) 1183
MaeII ACGT 9 cut(s) 28, 593, 699, 703, 977, 1604, 4194, 4892, 5179
MfeI CAATTG 4 cut(s) 1664, 1882, 2886, 4210
MflI RGATCY 3 cut(s) 1347, 1526, 3877
MhlI GDGCHC 8 cut(s) 180, 274, 1167, 1369, 2818, 3801, 4037, 4137
MluI ACGCGT 1 cut(s) 3471
MmeI TCCRAC 6 cut(s) 1065, 2432, 3771, 3788, 4007, 5112
Mph1103I ATGCAT 1 cut(s) 1523
MroI TCCGGA 1 cut(s) 2385
MroXI GAANNNNTTC 7 cut(s) 1077, 1142, 2018, 2427, 3408, 3750, 4534
MslI CAYNNNNRTG 3 cut(s) 4676, 4995, 5156
MspA1I CMGCKG 2 cut(s) 643, 4834
MspCI CTTAAG 2 cut(s) 1243, 3053
MspI CCGG 4 cut(s) 890, 2386, 3304, 5166
MspR9I CCNGG 9 cut(s) 316, 891, 1185, 1968, 2561, 3168, 3203, 3305, 4038
MunI CAATTG 4 cut(s) 1664, 1882, 2886, 4210
Mva1269I GAATGC 9 cut(s) 193, 1144, 1435, 1523, 2097, 2200, 2427, 3271, 4256
MvaI CCWGG 7 cut(s) 316, 1185, 1968, 2561, 3168, 3203, 4038
MvnI CGCG 1 cut(s) 3473
MwoI GCNNNNNNNGC 9 cut(s) 11, 20, 345, 649, 661, 2697, 3218, 4263, 5026
NciI CCSGG 2 cut(s) 891, 3305
NdeI CATATG 2 cut(s) 1924, 2632
NmeAIII GCCGAG 1 cut(s) 166
NmuCI GTSAC 3 cut(s) 124, 1018, 3285
NsbI TGCGCA 2 cut(s) 2007, 2701
NsiI ATGCAT 1 cut(s) 1523
NspI RCATGY 6 cut(s) 770, 1340, 2534, 2961, 4570, 4675
PacI TTAATTAA 1 cut(s) 3096
PaeI GCATGC 1 cut(s) 4570
PaeR7I CTCGAG 1 cut(s) 4423
PagI TCATGA 2 cut(s) 2332, 4072
PaqCI CACCTGC 1 cut(s) 3696
PceI AGGCCT 1 cut(s) 2354
PciI ACATGT 1 cut(s) 4671
PciSI GCTCTTC 1 cut(s) 3168
PcsI WCGNNNNNNNCGW 1 cut(s) 5107
PctI GAATGC 9 cut(s) 193, 1144, 1435, 1523, 2097, 2200, 2427, 3271, 4256
PdmI GAANNNNTTC 7 cut(s) 1077, 1142, 2018, 2427, 3408, 3750, 4534
Pfl23II CGTACG 1 cut(s) 700
PflFI GACNNNGTC 2 cut(s) 2084, 3683
PfoI TCCNGGA 2 cut(s) 314, 3303
Ppu21I YACGTR 3 cut(s) 700, 704, 4195
PpuMI RGGWCCY 1 cut(s) 5093
PscI ACATGT 1 cut(s) 4671
Psp124BI GAGCTC 3 cut(s) 180, 1167, 4137
Psp5II RGGWCCY 1 cut(s) 5093
Psp6I CCWGG 7 cut(s) 314, 1183, 1966, 2559, 3166, 3201, 4036
PspEI GGTNACC 2 cut(s) 1018, 3386
PspFI CCCAGC 1 cut(s) 3800
PspGI CCWGG 7 cut(s) 314, 1183, 1966, 2559, 3166, 3201, 4036
PspLI CGTACG 1 cut(s) 700
PspOMI GGGCCC 2 cut(s) 1365, 4033
PspPPI RGGWCCY 1 cut(s) 5093
PstI CTGCAG 4 cut(s) 1329, 3088, 3516, 4948
PstNI CAGNNNCTG 1 cut(s) 1530
PsuI RGATCY 3 cut(s) 1347, 1526, 3877
PsyI GACNNNGTC 2 cut(s) 2084, 3683
PvuII CAGCTG 1 cut(s) 643
RsaI GTAC 9 cut(s) 702, 1475, 1511, 1672, 1803, 1918, 4068, 4727, 4780
RsaNI GTAC 9 cut(s) 701, 1474, 1510, 1671, 1802, 1917, 4067, 4726, 4779
RseI CAYNNNNRTG 3 cut(s) 4676, 4995, 5156
SacI GAGCTC 3 cut(s) 180, 1167, 4137
SapI GCTCTTC 1 cut(s) 3168
ScrFI CCNGG 9 cut(s) 316, 891, 1185, 1968, 2561, 3168, 3203, 3305, 4038
SduI GDGCHC 8 cut(s) 180, 274, 1167, 1369, 2818, 3801, 4037, 4137
SexAI ACCWGGT 1 cut(s) 1183
SfcI CTRYAG 5 cut(s) 1325, 2731, 3084, 3512, 4944
Sfr274I CTCGAG 1 cut(s) 4423
SinI GGWCC 4 cut(s) 60, 1447, 4658, 5093
SlaI CTCGAG 1 cut(s) 4423
SmiMI CAYNNNNRTG 3 cut(s) 4676, 4995, 5156
SnaBI TACGTA 1 cut(s) 700
SpeI ACTAGT 2 cut(s) 128, 4762
SphI GCATGC 1 cut(s) 4570
SseBI AGGCCT 1 cut(s) 2354
SsiI CCGC 7 cut(s) 33, 1128, 1686, 1694, 2063, 3574, 4834
SstI GAGCTC 3 cut(s) 180, 1167, 4137
StuI AGGCCT 1 cut(s) 2354
StyD4I CCNGG 9 cut(s) 314, 889, 1183, 1966, 2559, 3166, 3201, 3303, 4036
StyI CCWWGG 2 cut(s) 3116, 3270
TaiI ACGT 9 cut(s) 31, 596, 702, 706, 980, 1607, 4197, 4895, 5182
TaqI TCGA 8 cut(s) 108, 332, 2431, 2984, 3486, 4424, 4431, 5067
TaqII GACCGA 1 cut(s) 1609
TatI WGTACW 7 cut(s) 1473, 1670, 1801, 1916, 4066, 4725, 4778
TscAI CASTG 9 cut(s) 760, 1468, 2276, 3199, 3294, 4713, 4791, 4948, 5077
TseFI GTSAC 3 cut(s) 124, 1018, 3285
Tsp45I GTSAC 3 cut(s) 124, 1018, 3285
TspGWI ACGGA 4 cut(s) 468, 1739, 4171, 4623
TspRI CASTG 9 cut(s) 760, 1468, 2276, 3199, 3294, 4713, 4791, 4948, 5077
Tth111I GACNNNGTC 2 cut(s) 2084, 3683
Vha464I CTTAAG 2 cut(s) 1243, 3053
VpaK11BI GGWCC 4 cut(s) 60, 1447, 4658, 5093
XagI CCTNNNNNAGG 2 cut(s) 2121, 3585
XbaI TCTAGA 1 cut(s) 3625
XceI RCATGY 6 cut(s) 770, 1340, 2534, 2961, 4570, 4675
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XhoI CTCGAG 1 cut(s) 4423
XmiI GTMKAC 2 cut(s) 1560, 4278
XmnI GAANNNNTTC 7 cut(s) 1077, 1142, 2018, 2427, 3408, 3750, 4534
ZraI GACGTC 2 cut(s) 1605, 5180
Zsp2I ATGCAT 1 cut(s) 1523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.