Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003946

Physical Location & Seq

Genomic Coordinates Forward (+)

6188945 .. 6191444

Gene Structure 2500 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1075800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2385 bp

ATGAAGACATACTTGGGTTATTGTACTCCAAGACAGGCCATTCTTACAAACGAAACAGGGGAAGGTATTTTTGATATGATGAAGCATATTCTGGAGGATATCTCGATTTTGGACCTGGAATTTTACAGTCATCGAATCATGCTTTATCAAGATGAATTGGAATTCCTTGGTGTGGGGATTAGATCAGTAGATGTGCAGAGATTGGTTACCAATCGCTTTATATCTCTTGCTTCTCCTGGAATGAGCAAAGAGTATACATTTTCTTTGCTGACTTTCATTACCTTCTCTAAAGCGAGGGGTATGCTTGATATGAATTGGTTGGCTGTTATGAAGGAGAAGAAGTGGCTGAAGACTCATAAGGGCTATAATGCTCCTGAGGTATCTATCATCTTGCCTTCTGAGATAGACACTCAATCCCGCTTGAAAGTAACAGATCTTCCTATTGTTGACGATATGGGGTTCTTTGTTGAGAATGAAATTAGGCATTTTGTTGATGAATTGAACGTAATGGGAGTGGGAGTTGATACTGCCAGAGCAACTGACTTGGTAGCTACTCAGTTTAATTCTCTCTTGCATTCTTCTAGCTGCTTGGCTCCTGAGAAAGTGATGGCACTGTTAGGTTGCATTCGAGAACTGAGTCAAACAATGCCTTTACCATGTCCTGAACTGACTTGGTTGTCGTCTGAGCAGTGGTTGAAGACACGACATGGTTATAGAAGTCCTAGCGAATCAATTATTTTCAGCTCAAAATGGGGGTCAATCTCGTGGTTTGTTGATCTTCCTATCATCGATGATACAAACTATGGCATCGGAATATATAAGTTCAGGGATGAACTTCAGAAGTTATGTGTCATCACAGATTTTGAAGGGGGAGCTCTGTTTGTAGTTAAAGGTCTCCGTATCCCCTTCGAAGCTGAACTAGTTGGTGCAGATGGTATGGTATCATTGCTAGAATGCATCAAGTCTCTGATGTCAAGGCCTCCTGATGAGCTTTTACTGACTGACTTTCTCAACAACATTGCAAAAAGCAGGTGTTTGAAGACTGGGGATGGTTACAAAACTCCGGAAGAGTGCATTCTGTTTGATCCAGCTTGGGAGAGTATTTTGAAGAGGTCCGATGCACCAAAAATTACAATTGATGAAAAGTTTTACGGAACTGATATTTTGGCATACAAGGATCAGTTAAGAGGTATTGGTGCGAATGTGGATCCCTTAGGCGTTTGTTCTCTCATTTCGGGGCTTCTAGTTTCACTCACAGATACATCTTCAATTACAAGAATTTATAGCTTTCTCAGTAAGTTCCACTGGCACCCGGAAGCAGTGAGCAATTTGCAGGTGTGGATACCTAATCCAAATGGTACAGGTGTATGGGTTAATTCTCAGTATTGCGTACTTTATGATAGGAACGACCGGTTTGGCTCCTGCTTGTTTTGTCTTGAAAACGTTTACAGAAAAGAGATCCTACCCATTTTTTCTTCAGCCTTTGGAGTGGCTGAAAATCCATCAACCAAAGATTACTTGCAGCTATGGAACAGCTGGGCCTTGAGGGGTAATGGGCAAGTGACTATGGAAGAATGCAGCTTCTTTTGGGAATATGTTTTCGATAACTGGAATCCTGAATTGGAGGATACTCTAAAGGGGAGCTTGACCAAATTACCTGCTACAATGTCCGTGGCCGAGGAAATATTTCTGATCAGCACAGGGGAAGTTTTCATTGGTGATGACTTGCAACTCAAGAAAAATTTCTCTAGTTTTGATAAGGCTCCTCTGTTTGTGTGGTTTCCGCATAGCAACTGCTTGTCATCAGTTCCTCCGATAAGACTTCATGAGTTTTATGAGTCACTCGGAGTTAGAAAGCTTTCTGAATCTGTTGAGTGCAACGTGAGTCATACAATATCATTGGAGCACTGGGAGAAGGTTGATCCAAGCGAGAGGTTAATAAGGAGGGGTTTAATGAAGATTATTCTGGGTTTTCTTGCTCGTCCTCATGTAAACATGCCCTTGAAGAGGAGACACGAGGCTGCAAAATCTATACTTGAACTGTCAGTGTACAAAGGTGACAAGCCGATTCAAGTCTGCTATCGGCTAATGCAATCTGCAGGTACAGTAGTGGAAGTAGAGAAACTAAAGCTGGTTCTTTGGGAGAAAAATTCGCAGCGCTTGCTCATTAAAAAATCAGCCTATGAAAGCGGAAAAACTGATATAGAATTTGTCACTTGTTTTGCTGATGAATTGGCGCAAGGGATGTTAGCACAGACAACACACACTGCTGCAGATACCTTGAGCAGGATTATCCAAATGGGATTCATTTATTTATTGAAGATGCACTGTTTCTTGATGCTGCATTCCTCCCGTCAGGAGGAGGTACTGGTCGTGTTTATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

795

Amino Acids

90.29

Weight (kDa)

5.54

Isoelectric Point (pI)

39.57

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003946)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g12160 FvH4_4g12170
malus_domestica	MD08G1075700.v1.1 MD08G1075800.v1.1 MD08G1076000.v1.1 MD15G1063200.v1.1
prunus_persica	Prupe.1G417200_v2.0.a1
pyrus_communis	pycom08g06260 pycom15g05980
rosa_chinensis	RchiOBHm_Chr4g0412151 RchiOBHm_Chr6g0275121
rosa_laevigata	RLG00000008313 RLG00000008314 RLG00000013495 RLG00000024070
rosa_multiflora	Rmu_sc0003510.1_g000026 Rmu_sc0004221.1_g000010 Rmu_sc0006012.1_g000009 Rmu_sc0011581.1_g000007 Rmu_ssc0000041.1_g000002
rosa_roxburghii	Rroxscaffold_5G00356420 Rroxscaffold_6G00408830
rosa_rugosa	Rorug03G0126700 Rorug04G0105600 Rorug06G0088600.1
rosa_samantha	Rh4AG171800 Rh4BG171000 Rh4BG171100 Rh4CG182600 Rh4DG166200 Rh6AG202600 Rh6DG198100
rosa_wichuraiana	Rw4G014340 Rw6G017590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1020, 1324
AasI	GACNNNNNNGTC	1 cut(s)	676
Acc36I	ACCTGC	4 cut(s)	1020, 1324, 1666, 2090
AccB1I	GGYRCC	1 cut(s)	1308
AccI	GTMKAC	1 cut(s)	254
AccIII	TCCGGA	1 cut(s)	1063
AciI	CCGC	3 cut(s)	418, 1784, 2190
AclI	AACGTT	1 cut(s)	1443
AclWI	GGATC	6 cut(s)	1079, 1185, 1202, 1215, 1453, 1916
AcoI	YGGCCR	1 cut(s)	1674
AcsI	RAATTY	6 cut(s)	119, 161, 1278, 1741, 2149, 2207
AcuI	CTGAAG	3 cut(s)	368, 821, 1461
AfaI	GTAC	6 cut(s)	25, 1360, 1392, 2051, 2104, 2367
AfeI	AGCGCT	1 cut(s)	2159
AfiI	CCNNNNNNNGG	4 cut(s)	172, 2007, 2286, 2359
AgeI	ACCGGT	1 cut(s)	1410
AhlI	ACTAGT	1 cut(s)	919
AjnI	CCWGG	2 cut(s)	114, 235
AjuI	GAANNNNNNNTTGG	5 cut(s)	28, 1604, 1636, 1698, 1730
Alw21I	GWGCWC	2 cut(s)	877, 1908
Alw26I	GTCTC	3 cut(s)	899, 969, 2005
AlwI	GGATC	6 cut(s)	1079, 1185, 1202, 1215, 1453, 1916
Aor13HI	TCCGGA	1 cut(s)	1063
Aor51HI	AGCGCT	1 cut(s)	2159
AoxI	GGCC	4 cut(s)	36, 977, 1539, 1674
ApeKI	GCWGC	7 cut(s)	585, 1522, 1578, 2021, 2155, 2270, 2341
ApoI	RAATTY	6 cut(s)	119, 161, 1278, 1741, 2149, 2207
ArsI	GACNNNNNNTTYG	2 cut(s)	740, 772
AsiGI	ACCGGT	1 cut(s)	1410
Asp700I	GAANNNNTTC	3 cut(s)	1686, 1742, 1858
AspLEI	GCGC	2 cut(s)	2160, 2239
AspS9I	GGNCC	3 cut(s)	112, 1113, 1539
AsuC2I	CCSGG	1 cut(s)	1313
AsuHPI	GGTGA	2 cut(s)	1730, 2069
AsuII	TTCGAA	1 cut(s)	909
AvaII	GGWCC	2 cut(s)	112, 1113
AxyI	CCTNAGG	2 cut(s)	375, 1213
BaeI	ACNNNNGTAYC	4 cut(s)	1350, 1383, 2094, 2127
BamHI	GGATCC	1 cut(s)	1207
BanI	GGYRCC	1 cut(s)	1308
BanII	GRGCYC	1 cut(s)	877
BarI	GAAGNNNNNNTAC	2 cut(s)	420, 452
BauI	CACGAG	2 cut(s)	763, 2015
BbsI	GAAGAC	4 cut(s)	11, 356, 704, 1046
Bbv12I	GWGCWC	2 cut(s)	877, 1908
BbvI	GCAGC	7 cut(s)	572, 1534, 1590, 2008, 2167, 2257, 2328
BccI	CCATC	4 cut(s)	601, 926, 1043, 1510
BcgI	CGANNNNNNTGC	2 cut(s)	283, 317
BciT130I	CCWGG	2 cut(s)	116, 237
BciVI	GTATCC	3 cut(s)	911, 1335, 1621
BclI	TGATCA	1 cut(s)	1692
BcnI	CCSGG	1 cut(s)	1313
BcoDI	GTCTC	3 cut(s)	899, 969, 2005
BcuI	ACTAGT	1 cut(s)	919
BfaI	CTAG	6 cut(s)	582, 723, 920, 950, 1244, 1749
BfmI	CTRYAG	2 cut(s)	2097, 2271
BfoI	RGCGCY	1 cut(s)	2161
BfuAI	ACCTGC	4 cut(s)	1020, 1324, 1666, 2090
BfuI	GTATCC	3 cut(s)	911, 1335, 1621
BglII	AGATCT	1 cut(s)	433
BisI	GCNGC	7 cut(s)	586, 1523, 1579, 2022, 2156, 2271, 2342
BlsI	GCNGC	7 cut(s)	587, 1524, 1580, 2023, 2157, 2272, 2343
Bme1390I	CCNGG	3 cut(s)	116, 237, 1313
Bme18I	GGWCC	2 cut(s)	112, 1113
BmgT120I	GGNCC	3 cut(s)	112, 1113, 1539
BmiI	GGNNCC	5 cut(s)	594, 1209, 1310, 1420, 1764
BmrFI	CCNGG	3 cut(s)	116, 237, 1313
BmrI	ACTGGG	2 cut(s)	1053, 1918
BmsI	GCATC	5 cut(s)	816, 966, 1108, 2313, 2328
BmuI	ACTGGG	2 cut(s)	1053, 1918
BpiI	GAAGAC	4 cut(s)	11, 356, 704, 1046
BplI	GAGNNNNNCTC	2 cut(s)	86, 118
BpmI	CTGGAG	1 cut(s)	113
Bpu14I	TTCGAA	1 cut(s)	909
BpuEI	CTTGAG	3 cut(s)	1564, 1718, 2302
BpuMI	CCSGG	1 cut(s)	1313
Bsa29I	ATCGAT	1 cut(s)	789
BsaI	GGTCTC	1 cut(s)	899
BsaJI	CCNNGG	3 cut(s)	166, 1671, 1677
BsaWI	WCCGGW	2 cut(s)	1063, 1410
BsaXI	ACNNNNNCTCC	4 cut(s)	326, 356, 504, 534
Bsc4I	CCNNNNNNNGG	4 cut(s)	172, 2007, 2286, 2359
Bse118I	RCCGGY	1 cut(s)	1410
Bse1I	ACTGG	5 cut(s)	1048, 1310, 1613, 1913, 2373
Bse21I	CCTNAGG	2 cut(s)	375, 1213
Bse3DI	GCAATG	2 cut(s)	944, 1017
BseAI	TCCGGA	1 cut(s)	1063
BseBI	CCWGG	2 cut(s)	116, 237
BseCI	ATCGAT	1 cut(s)	789
BseDI	CCNNGG	3 cut(s)	166, 1671, 1677
BseGI	GGATG	3 cut(s)	835, 1054, 2250
BseLI	CCNNNNNNNGG	4 cut(s)	172, 2007, 2286, 2359
BseMI	GCAATG	2 cut(s)	944, 1017
BseMII	CTCAG	8 cut(s)	366, 390, 569, 588, 626, 675, 1306, 1394
BseNI	ACTGG	5 cut(s)	1048, 1310, 1613, 1913, 2373
BseRI	GAGGAG	3 cut(s)	1755, 2023, 2375
BseXI	GCAGC	7 cut(s)	572, 1534, 1590, 2008, 2167, 2257, 2328
BseYI	CCCAGC	1 cut(s)	1536
BsgI	GTGCAG	2 cut(s)	215, 948
Bsh1285I	CGRYCG	1 cut(s)	1411
BshFI	GGCC	4 cut(s)	38, 979, 1541, 1676
BshNI	GGYRCC	1 cut(s)	1308
BshTI	ACCGGT	1 cut(s)	1410
BshVI	ATCGAT	1 cut(s)	789
BsiEI	CGRYCG	1 cut(s)	1411
BsiHKAI	GWGCWC	2 cut(s)	877, 1908
BsiSI	CCGG	3 cut(s)	1064, 1313, 1411
BslI	CCNNNNNNNGG	4 cut(s)	172, 2007, 2286, 2359
BsmAI	GTCTC	3 cut(s)	899, 969, 2005
BsmI	GAATGC	6 cut(s)	574, 624, 959, 1074, 1580, 2344
BsnI	GGCC	4 cut(s)	38, 979, 1541, 1676
Bso31I	GGTCTC	1 cut(s)	899
Bsp119I	TTCGAA	1 cut(s)	909
Bsp1286I	GDGCHC	2 cut(s)	877, 1908
Bsp13I	TCCGGA	1 cut(s)	1063
Bsp1407I	TGTACA	1 cut(s)	2049
Bsp143I	GATC	9 cut(s)	182, 433, 775, 1084, 1177, 1207, 1458, 1692, 1921
BspACI	CCGC	3 cut(s)	418, 1784, 2190
BspANI	GGCC	4 cut(s)	38, 979, 1541, 1676
BspCNI	CTCAG	8 cut(s)	367, 391, 568, 589, 627, 676, 1305, 1393
BspDI	ATCGAT	1 cut(s)	789
BspEI	TCCGGA	1 cut(s)	1063
BspHI	TCATGA	1 cut(s)	1825
BspLI	GGNNCC	5 cut(s)	594, 1209, 1310, 1420, 1764
BspMAI	CTGCAG	2 cut(s)	2101, 2275
BspMI	ACCTGC	4 cut(s)	1020, 1324, 1666, 2090
BspPI	GGATC	6 cut(s)	1079, 1185, 1202, 1215, 1453, 1916
BspT104I	TTCGAA	1 cut(s)	909
BspT107I	GGYRCC	1 cut(s)	1308
BspTNI	GGTCTC	1 cut(s)	899
BsrDI	GCAATG	2 cut(s)	944, 1017
BsrFI	RCCGGY	1 cut(s)	1410
BsrGI	TGTACA	1 cut(s)	2049
BsrI	ACTGG	5 cut(s)	1048, 1310, 1613, 1913, 2373
BssAI	RCCGGY	1 cut(s)	1410
BssECI	CCNNGG	3 cut(s)	166, 1671, 1677
BssMI	GATC	9 cut(s)	182, 433, 775, 1084, 1177, 1207, 1458, 1692, 1921
BssNAI	GTATAC	1 cut(s)	255
BssSI	CACGAG	2 cut(s)	763, 2015
BssT1I	CCWWGG	1 cut(s)	166
Bst1107I	GTATAC	1 cut(s)	255
Bst2BI	CACGAG	2 cut(s)	763, 2015
Bst2UI	CCWGG	2 cut(s)	116, 237
Bst4CI	ACNGT	5 cut(s)	128, 615, 2043, 2107, 2330
Bst6I	CTCTTC	3 cut(s)	1062, 1103, 2000
BstAPI	GCANNNNNTGC	1 cut(s)	2161
BstAUI	TGTACA	1 cut(s)	2049
BstBI	TTCGAA	1 cut(s)	909
BstC8I	GCNNGC	1 cut(s)	2162
BstDEI	CTNAG	9 cut(s)	375, 399, 555, 597, 635, 684, 1213, 1292, 1380
BstDSI	CCRYGG	1 cut(s)	1671
BstEII	GGTNACC	1 cut(s)	205
BstENI	CCTNNNNNAGG	2 cut(s)	2005, 2284
BstF5I	GGATG	3 cut(s)	835, 1054, 2250
BstH2I	RGCGCY	1 cut(s)	2161
BstHHI	GCGC	2 cut(s)	2160, 2239
BstKTI	GATC	9 cut(s)	185, 436, 778, 1087, 1180, 1210, 1461, 1695, 1924
BstMAI	GTCTC	3 cut(s)	899, 969, 2005
BstMBI	GATC	9 cut(s)	182, 433, 775, 1084, 1177, 1207, 1458, 1692, 1921
BstMCI	CGRYCG	1 cut(s)	1411
BstMWI	GCNNNNNNNGC	1 cut(s)	2161
BstNI	CCWGG	2 cut(s)	116, 237
BstNSI	RCATGY	1 cut(s)	1999
BstPI	GGTNACC	1 cut(s)	205
BstSCI	CCNGG	3 cut(s)	114, 235, 1311
BstSFI	CTRYAG	2 cut(s)	2097, 2271
BstV1I	GCAGC	7 cut(s)	572, 1534, 1590, 2008, 2167, 2257, 2328
BstV2I	GAAGAC	4 cut(s)	11, 356, 704, 1046
BstX2I	RGATCY	3 cut(s)	433, 1207, 1458
BstYI	RGATCY	3 cut(s)	433, 1207, 1458
BstZ17I	GTATAC	1 cut(s)	255
Bsu15I	ATCGAT	1 cut(s)	789
Bsu36I	CCTNAGG	2 cut(s)	375, 1213
BsuI	GTATCC	3 cut(s)	911, 1335, 1621
BsuRI	GGCC	4 cut(s)	38, 979, 1541, 1676
BsuTUI	ATCGAT	1 cut(s)	789
BtgI	CCRYGG	1 cut(s)	1671
BtsCI	GGATG	3 cut(s)	835, 1054, 2250
BtsI	GCAGTG	3 cut(s)	695, 1326, 2265
BtsIMutI	CAGTG	8 cut(s)	611, 695, 1303, 1326, 1906, 2052, 2265, 2326
BveI	ACCTGC	4 cut(s)	1020, 1324, 1666, 2090
Cac8I	GCNNGC	1 cut(s)	2162
CciI	TCATGA	1 cut(s)	1825
CfoI	GCGC	2 cut(s)	2160, 2239
Cfr10I	RCCGGY	1 cut(s)	1410
Cfr13I	GGNCC	3 cut(s)	112, 1113, 1539
ClaI	ATCGAT	1 cut(s)	789
Csp6I	GTAC	6 cut(s)	24, 1359, 1391, 2050, 2103, 2366
CspAI	ACCGGT	1 cut(s)	1410
CspCI	CAANNNNNGTGG	2 cut(s)	1653, 1688
CviAII	CATG	6 cut(s)	139, 657, 707, 1826, 1988, 1996
CviQI	GTAC	6 cut(s)	24, 1359, 1391, 2050, 2103, 2366
DdeI	CTNAG	9 cut(s)	375, 399, 555, 597, 635, 684, 1213, 1292, 1380
DpnI	GATC	9 cut(s)	184, 435, 777, 1086, 1179, 1209, 1460, 1694, 1923
DpnII	GATC	9 cut(s)	182, 433, 775, 1084, 1177, 1207, 1458, 1692, 1921
DrdI	GACNNNNNNGTC	1 cut(s)	676
DseDI	GACNNNNNNGTC	1 cut(s)	676
EaeI	YGGCCR	1 cut(s)	1674
Eam1104I	CTCTTC	3 cut(s)	1062, 1103, 2000
EarI	CTCTTC	3 cut(s)	1062, 1103, 2000
Ecl136II	GAGCTC	1 cut(s)	875
Eco130I	CCWWGG	1 cut(s)	166
Eco147I	AGGCCT	1 cut(s)	979
Eco24I	GRGCYC	1 cut(s)	877
Eco31I	GGTCTC	1 cut(s)	899
Eco32I	GATATC	1 cut(s)	100
Eco47I	GGWCC	2 cut(s)	112, 1113
Eco47III	AGCGCT	1 cut(s)	2159
Eco53kI	GAGCTC	1 cut(s)	875
Eco57I	CTGAAG	3 cut(s)	368, 821, 1461
Eco81I	CCTNAGG	2 cut(s)	375, 1213
Eco91I	GGTNACC	1 cut(s)	205
EcoICRI	GAGCTC	1 cut(s)	875
EcoNI	CCTNNNNNAGG	2 cut(s)	2005, 2284
EcoO65I	GGTNACC	1 cut(s)	205
EcoRI	GAATTC	1 cut(s)	161
EcoRII	CCWGG	2 cut(s)	114, 235
EcoRV	GATATC	1 cut(s)	100
EcoT14I	CCWWGG	1 cut(s)	166
EcoT22I	ATGCAT	1 cut(s)	959
EcoT38I	GRGCYC	1 cut(s)	877
ErhI	CCWWGG	1 cut(s)	166
FaeI	CATG	6 cut(s)	142, 660, 710, 1829, 1991, 1999
FalI	AAGNNNNNCTT	3 cut(s)	28, 1628, 1660
FatI	CATG	6 cut(s)	138, 656, 706, 1825, 1987, 1995
FauI	CCCGC	1 cut(s)	425
FbaI	TGATCA	1 cut(s)	1692
FblI	GTMKAC	1 cut(s)	254
Fnu4HI	GCNGC	7 cut(s)	586, 1523, 1579, 2022, 2156, 2271, 2342
FokI	GGATG	3 cut(s)	842, 1061, 2257
FriOI	GRGCYC	1 cut(s)	877
Fsp4HI	GCNGC	7 cut(s)	586, 1523, 1579, 2022, 2156, 2271, 2342
FspBI	CTAG	6 cut(s)	582, 723, 920, 950, 1244, 1749
GlaI	GCGC	2 cut(s)	2159, 2238
GluI	GCNGC	7 cut(s)	586, 1523, 1579, 2022, 2156, 2271, 2342
GsaI	CCCAGC	1 cut(s)	1540
GsuI	CTGGAG	1 cut(s)	113
HaeII	RGCGCY	1 cut(s)	2161
HaeIII	GGCC	4 cut(s)	38, 979, 1541, 1676
HapII	CCGG	3 cut(s)	1064, 1313, 1411
HhaI	GCGC	2 cut(s)	2160, 2239
Hin1II	CATG	6 cut(s)	142, 660, 710, 1829, 1991, 1999
Hin6I	GCGC	2 cut(s)	2158, 2237
HinP1I	GCGC	2 cut(s)	2158, 2237
HincII	GTYRAC	1 cut(s)	448
HindII	GTYRAC	1 cut(s)	448
HindIII	AAGCTT	1 cut(s)	1856
HpaII	CCGG	3 cut(s)	1064, 1313, 1411
HphI	GGTGA	2 cut(s)	1730, 2069
Hpy166II	GTNNAC	5 cut(s)	255, 448, 1447, 1993, 2050
Hpy8I	GTNNAC	5 cut(s)	255, 448, 1447, 1993, 2050
HpyAV	CCTTC	7 cut(s)	56, 292, 325, 405, 860, 916, 1909
HpyCH4III	ACNGT	5 cut(s)	128, 615, 2043, 2107, 2330
HpyCH4IV	ACGT	3 cut(s)	504, 1443, 1881
HpyF10VI	GCNNNNNNNGC	1 cut(s)	2161
HpyF3I	CTNAG	9 cut(s)	375, 399, 555, 597, 635, 684, 1213, 1292, 1380
HpySE526I	ACGT	3 cut(s)	504, 1443, 1881
Hsp92II	CATG	6 cut(s)	142, 660, 710, 1829, 1991, 1999
HspAI	GCGC	2 cut(s)	2158, 2237
Kpn2I	TCCGGA	1 cut(s)	1063
Ksp22I	TGATCA	1 cut(s)	1692
Kzo9I	GATC	9 cut(s)	182, 433, 775, 1084, 1177, 1207, 1458, 1692, 1921
LmnI	GCTCC	7 cut(s)	376, 598, 872, 1424, 1641, 1768, 1903
Lsp1109I	GCAGC	7 cut(s)	572, 1534, 1590, 2008, 2167, 2257, 2328
LweI	GCATC	5 cut(s)	816, 966, 1108, 2313, 2328
MaeI	CTAG	6 cut(s)	582, 723, 920, 950, 1244, 1749
MaeII	ACGT	3 cut(s)	504, 1443, 1881
MaeIII	GTNAC	7 cut(s)	205, 427, 1052, 1561, 1839, 2057, 2212
MalI	GATC	9 cut(s)	184, 435, 777, 1086, 1179, 1209, 1460, 1694, 1923
MboI	GATC	9 cut(s)	182, 433, 775, 1084, 1177, 1207, 1458, 1692, 1921
MfeI	CAATTG	1 cut(s)	1134
MflI	RGATCY	3 cut(s)	433, 1207, 1458
MhlI	GDGCHC	2 cut(s)	877, 1908
MlyI	GAGTC	4 cut(s)	346, 646, 1847, 1894
Mph1103I	ATGCAT	1 cut(s)	959
MroI	TCCGGA	1 cut(s)	1063
MroXI	GAANNNNTTC	3 cut(s)	1686, 1742, 1858
MseI	TTAA	7 cut(s)	561, 888, 1184, 1374, 1937, 1952, 2169
MspA1I	CMGCKG	1 cut(s)	1536
MspI	CCGG	3 cut(s)	1064, 1313, 1411
MspR9I	CCNGG	3 cut(s)	116, 237, 1313
MunI	CAATTG	1 cut(s)	1134
Mva1269I	GAATGC	6 cut(s)	574, 624, 959, 1074, 1580, 2344
MvaI	CCWGG	2 cut(s)	116, 237
MwoI	GCNNNNNNNGC	1 cut(s)	2161
NciI	CCSGG	1 cut(s)	1313
NdeII	GATC	9 cut(s)	182, 433, 775, 1084, 1177, 1207, 1458, 1692, 1921
NlaIII	CATG	6 cut(s)	142, 660, 710, 1829, 1991, 1999
NlaIV	GGNNCC	5 cut(s)	594, 1209, 1310, 1420, 1764
NmeAIII	GCCGAG	1 cut(s)	1702
NmuCI	GTSAC	4 cut(s)	1561, 1839, 2057, 2212
NsiI	ATGCAT	1 cut(s)	959
NspI	RCATGY	1 cut(s)	1999
NspV	TTCGAA	1 cut(s)	909
PagI	TCATGA	1 cut(s)	1825
PaqCI	CACCTGC	2 cut(s)	1020, 1324
PceI	AGGCCT	1 cut(s)	979
PctI	GAATGC	6 cut(s)	574, 624, 959, 1074, 1580, 2344
PdmI	GAANNNNTTC	3 cut(s)	1686, 1742, 1858
PfeI	GAWTC	6 cut(s)	135, 728, 1612, 1865, 2068, 2304
PfoI	TCCNGGA	1 cut(s)	235
PinAI	ACCGGT	1 cut(s)	1410
PkrI	GCNGC	7 cut(s)	587, 1524, 1580, 2023, 2157, 2272, 2343
PleI	GAGTC	4 cut(s)	346, 645, 1846, 1893
PpsI	GAGTC	4 cut(s)	346, 645, 1846, 1893
Psp124BI	GAGCTC	1 cut(s)	877
Psp1406I	AACGTT	1 cut(s)	1443
Psp6I	CCWGG	2 cut(s)	114, 235
PspEI	GGTNACC	1 cut(s)	205
PspFI	CCCAGC	1 cut(s)	1536
PspGI	CCWGG	2 cut(s)	114, 235
PspN4I	GGNNCC	5 cut(s)	594, 1209, 1310, 1420, 1764
PspPI	GGNCC	3 cut(s)	112, 1113, 1539
PstI	CTGCAG	2 cut(s)	2101, 2275
PsuI	RGATCY	3 cut(s)	433, 1207, 1458
PvuII	CAGCTG	1 cut(s)	1536
RsaI	GTAC	6 cut(s)	25, 1360, 1392, 2051, 2104, 2367
RsaNI	GTAC	6 cut(s)	24, 1359, 1391, 2050, 2103, 2366
SacI	GAGCTC	1 cut(s)	877
SaqAI	TTAA	7 cut(s)	561, 888, 1184, 1374, 1937, 1952, 2169
SatI	GCNGC	7 cut(s)	586, 1523, 1579, 2022, 2156, 2271, 2342
Sau3AI	GATC	9 cut(s)	182, 433, 775, 1084, 1177, 1207, 1458, 1692, 1921
Sau96I	GGNCC	3 cut(s)	112, 1113, 1539
SchI	GAGTC	4 cut(s)	346, 646, 1847, 1894
ScrFI	CCNGG	3 cut(s)	116, 237, 1313
SduI	GDGCHC	2 cut(s)	877, 1908
SfaNI	GCATC	5 cut(s)	816, 966, 1108, 2313, 2328
SfcI	CTRYAG	2 cut(s)	2097, 2271
SfuI	TTCGAA	1 cut(s)	909
SinI	GGWCC	2 cut(s)	112, 1113
SmlI	CTYRAG	3 cut(s)	1543, 1733, 2281
SmoI	CTYRAG	3 cut(s)	1543, 1733, 2281
SpeI	ACTAGT	1 cut(s)	919
SseBI	AGGCCT	1 cut(s)	979
SsiI	CCGC	3 cut(s)	418, 1784, 2190
SspI	AATATT	1 cut(s)	1686
SspMI	CTAG	6 cut(s)	582, 723, 920, 950, 1244, 1749
SstI	GAGCTC	1 cut(s)	877
StuI	AGGCCT	1 cut(s)	979
StyD4I	CCNGG	3 cut(s)	114, 235, 1311
StyI	CCWWGG	1 cut(s)	166
TaaI	ACNGT	5 cut(s)	128, 615, 2043, 2107, 2330
TaiI	ACGT	3 cut(s)	507, 1446, 1884
TaqI	TCGA	6 cut(s)	104, 133, 628, 789, 909, 1602
TatI	WGTACW	2 cut(s)	23, 2049
TfiI	GAWTC	6 cut(s)	135, 728, 1612, 1865, 2068, 2304
Tru1I	TTAA	7 cut(s)	561, 888, 1184, 1374, 1937, 1952, 2169
Tru9I	TTAA	7 cut(s)	561, 888, 1184, 1374, 1937, 1952, 2169
TscAI	CASTG	8 cut(s)	618, 695, 1310, 1326, 1913, 2052, 2272, 2333
TseFI	GTSAC	4 cut(s)	1561, 1839, 2057, 2212
TseI	GCWGC	7 cut(s)	585, 1522, 1578, 2021, 2155, 2270, 2341
Tsp45I	GTSAC	4 cut(s)	1561, 1839, 2057, 2212
TspGWI	ACGGA	3 cut(s)	887, 1167, 1660
TspRI	CASTG	8 cut(s)	618, 695, 1310, 1326, 1913, 2052, 2272, 2333
VpaK11BI	GGWCC	2 cut(s)	112, 1113
XagI	CCTNNNNNAGG	2 cut(s)	2005, 2284
XapI	RAATTY	6 cut(s)	119, 161, 1278, 1741, 2149, 2207
XceI	RCATGY	1 cut(s)	1999
XmiI	GTMKAC	1 cut(s)	254
XmnI	GAANNNNTTC	3 cut(s)	1686, 1742, 1858
XspI	CTAG	6 cut(s)	582, 723, 920, 950, 1244, 1749
Zsp2I	ATGCAT	1 cut(s)	959

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.