MD08G1097700.v1.1

Calnexin homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003158
Physical Location & Seq
Reverse (-)
8214833 .. 8215978
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1097700.v1.1.491

Sequence Viewer

Length: 669 bp
ATGGCAGGAACACGGTGGCGTCGAATCACTGACCTCCTTCTTCTTCTGCTGCTGATTGGCTGCTCTGCTTCCCAGATCCACGCTTCTTCAGAATACGAAGTCGATTCGATCTTTTACGAGCCATTTTCTGAGTCGTTTGAAGGCAGATGGATTGTTTCTTCGAAAGATGAGTACCAAGGTTATGCAGTGGTGAAAGAGATGGACAAGGCTGTGAGCCTTGCTGATGGAACTGTTGTCCTACAGTTTGAGGTCCGGCTCCAGAACGGGCTTGAATGTGGCGGTGCTTACTTGAAGTACCTCCGGCCACAGGAGGCTGGATGGAAAGCGGAGGAATTTGACAATGAGTCGCCATATTCTATTATGTTTGGACCGGACAAATGTGGTTCCACAAACAAGGTTCATTTCATTTTTAAGCACAAGAACCCCAAGAGTGGAGAGTATGTTGAGCACCATCTCAAGGACCCTCCATCCGTGCCCTCCGATAAGCTGTCTCATGTTTACACTGCCATACTGAAACCTGATAACGAAGTTAGGATTTTGATAGATGGGGAGGAGAAGAAGAAGGCGAATTTCCTGTCGAATAAAGATTTTGAGCCTGCATTGATTCCAGCCAAGACAATTCCTGACCCAGAGGATCAAAAAAGCCAGAGGATTGGGATGAGCGGCTGA

Protein Analysis

223

Amino Acids

25.05

Weight (kDa)

5.45

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calreticulin PF00262 31 - 214 5.9e-54 Calreticulin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 663
AciI CCGC 3 cut(s) 279, 326, 663
AclWI GGATC 2 cut(s) 70, 642
AcoI YGGCCR 1 cut(s) 302
AcsI RAATTY 2 cut(s) 332, 568
AcuI CTGAAG 1 cut(s) 72
AcyI GRCGYC 1 cut(s) 19
AfaI GTAC 2 cut(s) 173, 296
AfiI CCNNNNNNNGG 3 cut(s) 307, 431, 457
AgsI TTSAA 3 cut(s) 140, 272, 292
AhdI GACNNNNNGTC 1 cut(s) 343
AluBI AGCT 1 cut(s) 487
AluI AGCT 1 cut(s) 487
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 1 cut(s) 495
AlwI GGATC 2 cut(s) 70, 642
AoxI GGCC 1 cut(s) 302
ApeKI GCWGC 2 cut(s) 49, 60
ApoI RAATTY 2 cut(s) 332, 568
ArsI GACNNNNNNTTYG 2 cut(s) 560, 592
AspS9I GGNCC 3 cut(s) 250, 368, 460
AsuHPI GGTGA 1 cut(s) 202
AsuII TTCGAA 1 cut(s) 161
AvaII GGWCC 3 cut(s) 250, 368, 460
BaeGI GKGCMC 1 cut(s) 477
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 2 cut(s) 36, 47
BccI CCATC 7 cut(s) 141, 193, 218, 312, 459, 475, 539
BcoDI GTCTC 1 cut(s) 495
BfmI CTRYAG 1 cut(s) 239
BisI GCNGC 3 cut(s) 50, 61, 664
BlsI GCNGC 3 cut(s) 51, 62, 665
Bme18I GGWCC 3 cut(s) 250, 368, 460
BmeRI GACNNNNNGTC 1 cut(s) 343
BmgT120I GGNCC 3 cut(s) 250, 368, 460
BmiI GGNNCC 3 cut(s) 257, 385, 462
BpmI CTGGAG 1 cut(s) 242
Bpu14I TTCGAA 1 cut(s) 161
BpuEI CTTGAG 1 cut(s) 440
BsaHI GRCGYC 1 cut(s) 19
BsaJI CCNNGG 1 cut(s) 175
BsaWI WCCGGW 1 cut(s) 370
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 431, 457
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 3 cut(s) 323, 467, 663
BseLI CCNNNNNNNGG 3 cut(s) 307, 431, 457
BseMII CTCAG 1 cut(s) 120
BseRI GAGGAG 1 cut(s) 566
BseSI GKGCMC 1 cut(s) 477
BseXI GCAGC 2 cut(s) 36, 47
BshFI GGCC 1 cut(s) 304
BsiHKAI GWGCWC 1 cut(s) 450
BsiSI CCGG 3 cut(s) 253, 301, 371
BslI CCNNNNNNNGG 3 cut(s) 307, 431, 457
BsmAI GTCTC 1 cut(s) 495
BsnI GGCC 1 cut(s) 304
Bsp119I TTCGAA 1 cut(s) 161
Bsp1286I GDGCHC 2 cut(s) 450, 477
Bsp143I GATC 3 cut(s) 75, 108, 634
BspACI CCGC 3 cut(s) 279, 326, 663
BspANI GGCC 1 cut(s) 304
BspCNI CTCAG 1 cut(s) 121
BspLI GGNNCC 3 cut(s) 257, 385, 462
BspPI GGATC 2 cut(s) 70, 642
BspT104I TTCGAA 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 663
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 3 cut(s) 75, 108, 634
BssNI GRCGYC 1 cut(s) 19
BssT1I CCWWGG 1 cut(s) 175
Bst4CI ACNGT 3 cut(s) 15, 232, 243
BstACI GRCGYC 1 cut(s) 19
BstBI TTCGAA 1 cut(s) 161
BstC8I GCNNGC 1 cut(s) 597
BstDEI CTNAG 1 cut(s) 129
BstF5I GGATG 3 cut(s) 323, 467, 663
BstKTI GATC 3 cut(s) 78, 111, 637
BstMAI GTCTC 1 cut(s) 495
BstMBI GATC 3 cut(s) 75, 108, 634
BstSFI CTRYAG 1 cut(s) 239
BstSLI GKGCMC 1 cut(s) 477
BstV1I GCAGC 2 cut(s) 36, 47
BstX2I RGATCY 1 cut(s) 75
BstXI CCANNNNNNTGG 1 cut(s) 653
BstYI RGATCY 1 cut(s) 75
BsuRI GGCC 1 cut(s) 304
BtsCI GGATG 3 cut(s) 323, 467, 663
BtsI GCAGTG 2 cut(s) 192, 501
BtsIMutI CAGTG 3 cut(s) 27, 192, 501
Cac8I GCNNGC 1 cut(s) 597
Cfr13I GGNCC 3 cut(s) 250, 368, 460
CseI GACGC 1 cut(s) 8
Csp6I GTAC 2 cut(s) 172, 295
CviAII CATG 1 cut(s) 494
CviQI GTAC 2 cut(s) 172, 295
DdeI CTNAG 1 cut(s) 129
DpnI GATC 3 cut(s) 77, 110, 636
DpnII GATC 3 cut(s) 75, 108, 634
DriI GACNNNNNGTC 1 cut(s) 343
EaeI YGGCCR 1 cut(s) 302
Eam1105I GACNNNNNGTC 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 175
Eco47I GGWCC 3 cut(s) 250, 368, 460
Eco57I CTGAAG 1 cut(s) 72
EcoO109I RGGNCCY 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 175
FaeI CATG 1 cut(s) 497
FaiI YATR 6 cut(s) 183, 352, 362, 441, 495, 509
FatI CATG 1 cut(s) 493
Fnu4HI GCNGC 3 cut(s) 50, 61, 664
FokI GGATG 2 cut(s) 330, 454
Fsp4HI GCNGC 3 cut(s) 50, 61, 664
GluI GCNGC 3 cut(s) 50, 61, 664
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 1 cut(s) 304
HapII CCGG 3 cut(s) 253, 301, 371
HgaI GACGC 1 cut(s) 8
Hin1I GRCGYC 1 cut(s) 19
Hin1II CATG 1 cut(s) 497
HinfI GANTC 5 cut(s) 24, 104, 131, 344, 604
HpaII CCGG 3 cut(s) 253, 301, 371
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 3 cut(s) 91, 130, 481
Hpy188III TCNNGA 2 cut(s) 259, 623
Hpy8I GTNNAC 1 cut(s) 499
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 3 cut(s) 47, 134, 556
HpyCH4III ACNGT 3 cut(s) 15, 232, 243
HpyCH4V TGCA 2 cut(s) 185, 599
HpyF3I CTNAG 1 cut(s) 129
Hsp92I GRCGYC 1 cut(s) 19
Hsp92II CATG 1 cut(s) 497
Kzo9I GATC 3 cut(s) 75, 108, 634
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 2 cut(s) 36, 47
MalI GATC 3 cut(s) 77, 110, 636
MbiI CCGCTC 1 cut(s) 663
MboI GATC 3 cut(s) 75, 108, 634
MboII GAAGA 6 cut(s) 32, 35, 78, 150, 568, 571
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 2 cut(s) 450, 477
MluCI AATT 3 cut(s) 332, 568, 618
MlyI GAGTC 2 cut(s) 140, 353
MseI TTAA 1 cut(s) 411
MspI CCGG 3 cut(s) 253, 301, 371
NdeII GATC 3 cut(s) 75, 108, 634
NlaIII CATG 1 cut(s) 497
NlaIV GGNNCC 3 cut(s) 257, 385, 462
NspV TTCGAA 1 cut(s) 161
PcsI WCGNNNNNNNCGW 1 cut(s) 19
PfeI GAWTC 3 cut(s) 24, 104, 604
PkrI GCNGC 3 cut(s) 51, 62, 665
PleI GAGTC 2 cut(s) 139, 352
PpsI GAGTC 2 cut(s) 139, 352
PpuMI RGGWCCY 1 cut(s) 460
Psp5II RGGWCCY 1 cut(s) 460
PspN4I GGNNCC 3 cut(s) 257, 385, 462
PspPI GGNCC 3 cut(s) 250, 368, 460
PspPPI RGGWCCY 1 cut(s) 460
PsuI RGATCY 1 cut(s) 75
RsaI GTAC 2 cut(s) 173, 296
RsaNI GTAC 2 cut(s) 172, 295
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 3 cut(s) 50, 61, 664
Sau3AI GATC 3 cut(s) 75, 108, 634
Sau96I GGNCC 3 cut(s) 250, 368, 460
SchI GAGTC 2 cut(s) 140, 353
SduI GDGCHC 2 cut(s) 450, 477
SetI ASST 7 cut(s) 36, 181, 252, 300, 399, 489, 520
SfcI CTRYAG 1 cut(s) 239
SfuI TTCGAA 1 cut(s) 161
SinI GGWCC 3 cut(s) 250, 368, 460
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 3 cut(s) 332, 568, 618
SsiI CCGC 3 cut(s) 279, 326, 663
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 3 cut(s) 15, 232, 243
TaqI TCGA 5 cut(s) 22, 102, 107, 161, 578
TasI AATT 3 cut(s) 332, 568, 618
TauI GCSGC 1 cut(s) 666
TfiI GAWTC 3 cut(s) 24, 104, 604
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 3 cut(s) 34, 192, 508
TseI GCWGC 2 cut(s) 49, 60
TspDTI ATGAA 2 cut(s) 389, 394
TspGWI ACGGA 1 cut(s) 460
TspRI CASTG 3 cut(s) 34, 192, 508
VpaK11BI GGWCC 3 cut(s) 250, 368, 460
XapI RAATTY 2 cut(s) 332, 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.