Rh7BG096200

Calnexin homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0003158
Physical Location & Seq
Reverse (-)
7101815 .. 7105181
3367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG096200.1

Sequence Viewer

Length: 1311 bp
ATGGCTGGGATTCCCTCCGGCGTCGTTTCGGTGCTGTTCGTGCTCTTTGCCTGCTTCTCTATTCAGCAGCTTTCTGCTTCCGACGATACGGTCTTTTACGATTCGTTCGACGAATCATTTGAAGGGCGTTGGACCGTGTCCGGCAAAGATGAATATCAAGGTGTTTGGAAGCACTCAAAGAGTGAGGGCCATGAGGATTTTGGGCTTCTTGTGAGTGAGAAGGCCAAGAAGTATGCAATAGTTAAGGAGCTTGATGAGCCTGTGAGCCTCAAGGATGGGACTGTTGTCCTTCAATTTGAGACTCGTCTCCAGAACGGGCTTGAATGTGGTGGCGCCTATCTGAAATACCTTCGACCCCAGGAGGCTGGATGGGAACCTAAGATATTTGACAATGAGTCTCCTTATTCTATCATGTTTGGACCCGACAAATGTGGGCTTACAAACAAGGTTCACTTCATTTTCAAGCATAAGAATCCCAAGAGTGGAGAGTATGTTGAACACCATCTTACCACCCCACCCTCTGTTCCAACTGACAAACTTTCTCATGTATACACTGCCATTATCAAACCTGATAACGAGTTGATAATTCTGGTTGATGGAGAGGAGAAGAAGAAGGCCAACTTTTTATCAACTGATGATTTTATGCCTCCACTTATCCCTGCCAAGACAATTCCTGATCCAGAAGATAAGAAGCCTGAGGATTGGGATGAGAGAGCCAAAATTCCGGATCCTAATGCAGTTAAACCTGATGATTGGGATGAGGATGCACCCATGGAAATTGAAGACGAGGAAGCAGAGAAACCTGAAGGATGGTTGGATGATGAGCCTGAGGAGGTAGATGATCCTGAAGCCACAAAACCAGAGGATTGGGATGACGAGGAGGATGGTGAGTGGGAGGCCCCTAAGATTGACAACCCCAAGTGTGTGACTGCCCCTGGTTGTGGTGAATGGAAGAGGCCAATGAAGAGGAATCCAGCTTACAAGGGAAAATGGCATGCACCACTCATCGATAACCCCAGTTACAAGGGTATTTGGAAGCCTCAGGAGATTCCTAACCCCAGTTACTTTGAGCTCGAGAAACCTAACTTCGAGCCCATTGCTGCTGTTGGTATTGAGATCTGGACCATGCAAGATGGTATATTGTTTGACAACATTTTGATAACAAAAGATGAGAAAGTTGCACAATCATACCGAGAGACCCAGTGGAAACCAAAATTTGAAGTTGAGAAGGAGAAAGAGAAGGCTGAGGAAGATGTTCCTAGTTCAGAAGGTCTATTAGCCAACGTCCAGGTACATGGTTTTGCAATTTAG

Protein Analysis

436

Amino Acids

49.56

Weight (kDa)

4.55

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calreticulin PF00262 32 - 387 1.3e-144 Calreticulin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 332
AccI GTMKAC 1 cut(s) 549
AccIII TCCGGA 1 cut(s) 724
AclWI GGATC 4 cut(s) 671, 722, 735, 836
AcsI RAATTY 2 cut(s) 720, 1214
AcuI CTGAAG 2 cut(s) 825, 867
AcyI GRCGYC 2 cut(s) 21, 333
AfaI GTAC 1 cut(s) 1293
AfiI CCNNNNNNNGG 2 cut(s) 482, 941
AgsI TTSAA 7 cut(s) 122, 293, 323, 463, 497, 782, 1220
AhdI GACNNNNNGTC 1 cut(s) 394
AjnI CCWGG 3 cut(s) 357, 934, 1287
AluBI AGCT 4 cut(s) 70, 250, 977, 1072
AluI AGCT 4 cut(s) 70, 250, 977, 1072
Alw21I GWGCWC 2 cut(s) 45, 1074
Alw26I GTCTC 4 cut(s) 293, 311, 402, 1190
AlwI GGATC 4 cut(s) 671, 722, 735, 836
Ama87I CYCGRG 1 cut(s) 1073
Aor13HI TCCGGA 1 cut(s) 724
AoxI GGCC 5 cut(s) 187, 222, 615, 897, 956
ApeKI GCWGC 2 cut(s) 67, 1100
ApoI RAATTY 2 cut(s) 720, 1214
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
Asp700I GAANNNNTTC 1 cut(s) 1254
AspLEI GCGC 1 cut(s) 335
AspS9I GGNCC 5 cut(s) 132, 187, 419, 898, 1122
AsuHPI GGTGA 2 cut(s) 899, 956
AvaI CYCGRG 1 cut(s) 1073
AvaII GGWCC 3 cut(s) 132, 419, 1122
AxyI CCTNAGG 3 cut(s) 696, 828, 1041
BamHI GGATCC 1 cut(s) 727
BanI GGYRCC 1 cut(s) 332
BanII GRGCYC 2 cut(s) 1074, 1095
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 2 cut(s) 45, 1074
BbvCI CCTCAGC 1 cut(s) 1245
BbvI GCAGC 2 cut(s) 79, 1087
BccI CCATC 7 cut(s) 269, 363, 510, 590, 804, 878, 1127
BcgI CGANNNNNNTGC 2 cut(s) 1079, 1113
BciT130I CCWGG 3 cut(s) 359, 936, 1289
BcoDI GTCTC 4 cut(s) 293, 311, 402, 1190
BfaI CTAG 1 cut(s) 1260
BfoI RGCGCY 1 cut(s) 336
BglII AGATCT 1 cut(s) 1116
BisI GCNGC 2 cut(s) 68, 1101
BlsI GCNGC 2 cut(s) 69, 1102
Bme1390I CCNGG 3 cut(s) 359, 936, 1289
Bme18I GGWCC 3 cut(s) 132, 419, 1122
BmeRI GACNNNNNGTC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 1073
BmgT120I GGNCC 5 cut(s) 132, 187, 419, 898, 1122
BmiI GGNNCC 5 cut(s) 334, 375, 421, 729, 900
BmrFI CCNGG 3 cut(s) 359, 936, 1289
BmrI ACTGGG 3 cut(s) 1011, 1053, 1195
BmsI GCATC 1 cut(s) 754
BmuI ACTGGG 3 cut(s) 1011, 1053, 1195
BoxI GACNNNNGTC 1 cut(s) 284
BpiI GAAGAC 1 cut(s) 789
BpmI CTGGAG 1 cut(s) 293
Bpu10I CCTNAGC 1 cut(s) 1245
BpuEI CTTGAG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 1008
BsaBI GATNNNNATC 1 cut(s) 153
BsaHI GRCGYC 2 cut(s) 21, 333
BsaI GGTCTC 1 cut(s) 1190
BsaJI CCNNGG 3 cut(s) 357, 771, 934
BsaWI WCCGGW 1 cut(s) 724
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bsc4I CCNNNNNNNGG 2 cut(s) 482, 941
Bse1I ACTGG 3 cut(s) 1017, 1059, 1201
Bse21I CCTNAGG 3 cut(s) 696, 828, 1041
Bse3DI GCAATG 1 cut(s) 1095
Bse8I GATNNNNATC 1 cut(s) 153
BseAI TCCGGA 1 cut(s) 724
BseBI CCWGG 3 cut(s) 359, 936, 1289
BseCI ATCGAT 1 cut(s) 1008
BseDI CCNNGG 3 cut(s) 357, 771, 934
BseGI GGATG 9 cut(s) 280, 374, 712, 763, 769, 815, 823, 877, 889
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 2 cut(s) 482, 941
BseMI GCAATG 1 cut(s) 1095
BseMII CTCAG 4 cut(s) 687, 819, 1055, 1236
BseNI ACTGG 3 cut(s) 1017, 1059, 1201
BseRI GAGGAG 3 cut(s) 617, 845, 893
BseXI GCAGC 2 cut(s) 79, 1087
BseYI CCCAGC 1 cut(s) 5
BshFI GGCC 5 cut(s) 189, 224, 617, 899, 958
BshNI GGYRCC 1 cut(s) 332
BshVI ATCGAT 1 cut(s) 1008
BsiHKAI GWGCWC 2 cut(s) 45, 1074
BsiHKCI CYCGRG 1 cut(s) 1073
BsiSI CCGG 3 cut(s) 18, 141, 725
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 2 cut(s) 482, 941
BsmAI GTCTC 4 cut(s) 293, 311, 402, 1190
BsmBI CGTCTC 1 cut(s) 311
BsmFI GGGAC 1 cut(s) 292
BsnI GGCC 5 cut(s) 189, 224, 617, 899, 958
Bso31I GGTCTC 1 cut(s) 1190
BsoBI CYCGRG 1 cut(s) 1073
Bsp1286I GDGCHC 3 cut(s) 45, 1074, 1095
Bsp13I TCCGGA 1 cut(s) 724
Bsp143I GATC 4 cut(s) 676, 727, 841, 1116
Bsp19I CCATGG 1 cut(s) 771
BspANI GGCC 5 cut(s) 189, 224, 617, 899, 958
BspCNI CTCAG 4 cut(s) 688, 820, 1054, 1237
BspDI ATCGAT 1 cut(s) 1008
BspEI TCCGGA 1 cut(s) 724
BspLI GGNNCC 5 cut(s) 334, 375, 421, 729, 900
BspPI GGATC 4 cut(s) 671, 722, 735, 836
BspT107I GGYRCC 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 1190
BsrDI GCAATG 1 cut(s) 1095
BsrI ACTGG 3 cut(s) 1017, 1059, 1201
BssECI CCNNGG 3 cut(s) 357, 771, 934
BssMI GATC 4 cut(s) 676, 727, 841, 1116
BssNAI GTATAC 1 cut(s) 550
BssNI GRCGYC 2 cut(s) 21, 333
BssT1I CCWWGG 1 cut(s) 771
Bst1107I GTATAC 1 cut(s) 550
Bst2UI CCWGG 3 cut(s) 359, 936, 1289
Bst4CI ACNGT 3 cut(s) 91, 136, 283
Bst6I CTCTTC 2 cut(s) 947, 959
BstACI GRCGYC 2 cut(s) 21, 333
BstC8I GCNNGC 2 cut(s) 52, 996
BstDEI CTNAG 6 cut(s) 378, 696, 828, 903, 1041, 1245
BstDSI CCRYGG 1 cut(s) 771
BstF5I GGATG 9 cut(s) 280, 374, 712, 763, 769, 815, 823, 877, 889
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 1 cut(s) 335
BstKTI GATC 4 cut(s) 679, 730, 844, 1119
BstMAI GTCTC 4 cut(s) 293, 311, 402, 1190
BstMBI GATC 4 cut(s) 676, 727, 841, 1116
BstMWI GCNNNNNNNGC 2 cut(s) 40, 256
BstNI CCWGG 3 cut(s) 359, 936, 1289
BstNSI RCATGY 1 cut(s) 998
BstPAI GACNNNNGTC 1 cut(s) 284
BstSCI CCNGG 3 cut(s) 357, 934, 1287
BstV1I GCAGC 2 cut(s) 79, 1087
BstV2I GAAGAC 1 cut(s) 789
BstX2I RGATCY 2 cut(s) 727, 1116
BstXI CCANNNNNNTGG 3 cut(s) 365, 867, 1295
BstYI RGATCY 2 cut(s) 727, 1116
BstZ17I GTATAC 1 cut(s) 550
Bsu15I ATCGAT 1 cut(s) 1008
Bsu36I CCTNAGG 3 cut(s) 696, 828, 1041
BsuRI GGCC 5 cut(s) 189, 224, 617, 899, 958
BsuTUI ATCGAT 1 cut(s) 1008
BtgI CCRYGG 1 cut(s) 771
BtsCI GGATG 9 cut(s) 280, 374, 712, 763, 769, 815, 823, 877, 889
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 2 cut(s) 552, 1208
Cac8I GCNNGC 2 cut(s) 52, 996
CfoI GCGC 1 cut(s) 335
Cfr13I GGNCC 5 cut(s) 132, 187, 419, 898, 1122
ClaI ATCGAT 1 cut(s) 1008
CseI GACGC 1 cut(s) 10
Csp6I GTAC 1 cut(s) 1292
CviAII CATG 7 cut(s) 191, 412, 545, 772, 995, 1126, 1295
CviQI GTAC 1 cut(s) 1292
DdeI CTNAG 6 cut(s) 378, 696, 828, 903, 1041, 1245
DinI GGCGCC 1 cut(s) 334
DpnI GATC 4 cut(s) 678, 729, 843, 1118
DpnII GATC 4 cut(s) 676, 727, 841, 1116
DrdI GACNNNNNNGTC 1 cut(s) 89
DriI GACNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 89
Eam1104I CTCTTC 2 cut(s) 947, 959
Eam1105I GACNNNNNGTC 1 cut(s) 394
EarI CTCTTC 2 cut(s) 947, 959
Ecl136II GAGCTC 1 cut(s) 1072
Eco130I CCWWGG 1 cut(s) 771
Eco24I GRGCYC 2 cut(s) 1074, 1095
Eco31I GGTCTC 1 cut(s) 1190
Eco47I GGWCC 3 cut(s) 132, 419, 1122
Eco53kI GAGCTC 1 cut(s) 1072
Eco57I CTGAAG 2 cut(s) 825, 867
Eco81I CCTNAGG 3 cut(s) 696, 828, 1041
Eco88I CYCGRG 1 cut(s) 1073
EcoICRI GAGCTC 1 cut(s) 1072
EcoO109I RGGNCCY 1 cut(s) 898
EcoRII CCWGG 3 cut(s) 357, 934, 1287
EcoT14I CCWWGG 1 cut(s) 771
EcoT38I GRGCYC 2 cut(s) 1074, 1095
EgeI GGCGCC 1 cut(s) 334
EheI GGCGCC 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 771
Esp3I CGTCTC 1 cut(s) 311
FaeI CATG 7 cut(s) 194, 415, 548, 775, 998, 1129, 1298
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 605, 637
FaqI GGGAC 1 cut(s) 292
FatI CATG 7 cut(s) 190, 411, 544, 771, 994, 1125, 1294
FblI GTMKAC 1 cut(s) 549
Fnu4HI GCNGC 2 cut(s) 68, 1101
FokI GGATG 9 cut(s) 287, 381, 719, 770, 776, 822, 830, 884, 896
FriOI GRGCYC 2 cut(s) 1074, 1095
Fsp4HI GCNGC 2 cut(s) 68, 1101
FspBI CTAG 1 cut(s) 1260
GlaI GCGC 1 cut(s) 334
GluI GCNGC 2 cut(s) 68, 1101
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 293
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 5 cut(s) 189, 224, 617, 899, 958
HapII CCGG 3 cut(s) 18, 141, 725
HgaI GACGC 1 cut(s) 10
HhaI GCGC 1 cut(s) 335
Hin1I GRCGYC 2 cut(s) 21, 333
Hin1II CATG 7 cut(s) 194, 415, 548, 775, 998, 1129, 1298
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HinfI GANTC 8 cut(s) 10, 101, 113, 301, 395, 472, 970, 1048
HpaII CCGG 3 cut(s) 18, 141, 725
HphI GGTGA 2 cut(s) 899, 956
Hpy166II GTNNAC 2 cut(s) 451, 550
Hpy188I TCNGA 3 cut(s) 82, 342, 1267
Hpy188III TCNNGA 8 cut(s) 310, 674, 680, 725, 845, 1043, 1075, 1120
Hpy8I GTNNAC 2 cut(s) 451, 550
Hpy99I CGWCG 3 cut(s) 26, 86, 113
HpyAV CCTTC 9 cut(s) 116, 214, 299, 359, 607, 800, 1222, 1234, 1262
HpyCH4III ACNGT 3 cut(s) 91, 136, 283
HpyCH4IV ACGT 1 cut(s) 1284
HpyCH4V TGCA 7 cut(s) 236, 737, 767, 998, 1129, 1181, 1304
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 256
HpyF3I CTNAG 6 cut(s) 378, 696, 828, 903, 1041, 1245
HpySE526I ACGT 1 cut(s) 1284
Hsp92I GRCGYC 2 cut(s) 21, 333
Hsp92II CATG 7 cut(s) 194, 415, 548, 775, 998, 1129, 1298
HspAI GCGC 1 cut(s) 333
KasI GGCGCC 1 cut(s) 332
Kpn2I TCCGGA 1 cut(s) 724
Kzo9I GATC 4 cut(s) 676, 727, 841, 1116
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 2 cut(s) 79, 1087
LweI GCATC 1 cut(s) 754
MaeI CTAG 1 cut(s) 1260
MaeII ACGT 1 cut(s) 1284
MaeIII GTNAC 3 cut(s) 925, 1019, 1061
MalI GATC 4 cut(s) 678, 729, 843, 1118
MboI GATC 4 cut(s) 676, 727, 841, 1116
MboII GAAGA 7 cut(s) 619, 622, 695, 794, 964, 976, 1262
MflI RGATCY 2 cut(s) 727, 1116
MhlI GDGCHC 3 cut(s) 45, 1074, 1095
MluCI AATT 7 cut(s) 293, 585, 669, 720, 777, 1214, 1305
Mly113I GGCGCC 1 cut(s) 333
MlyI GAGTC 2 cut(s) 295, 404
MmeI TCCRAC 4 cut(s) 105, 110, 551, 795
MroI TCCGGA 1 cut(s) 724
MroXI GAANNNNTTC 1 cut(s) 1254
MseI TTAA 2 cut(s) 243, 741
MspI CCGG 3 cut(s) 18, 141, 725
MspR9I CCNGG 3 cut(s) 359, 936, 1289
MvaI CCWGG 3 cut(s) 359, 936, 1289
MwoI GCNNNNNNNGC 2 cut(s) 40, 256
NarI GGCGCC 1 cut(s) 333
NcoI CCATGG 1 cut(s) 771
NdeII GATC 4 cut(s) 676, 727, 841, 1116
NlaIII CATG 7 cut(s) 194, 415, 548, 775, 998, 1129, 1298
NlaIV GGNNCC 5 cut(s) 334, 375, 421, 729, 900
NmuCI GTSAC 1 cut(s) 925
NspI RCATGY 1 cut(s) 998
PaeI GCATGC 1 cut(s) 998
PaeR7I CTCGAG 1 cut(s) 1073
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 1254
PfeI GAWTC 6 cut(s) 10, 101, 113, 472, 970, 1048
PflFI GACNNNGTC 1 cut(s) 136
PkrI GCNGC 2 cut(s) 69, 1102
PleI GAGTC 2 cut(s) 295, 403
PluTI GGCGCC 1 cut(s) 336
PpsI GAGTC 2 cut(s) 295, 403
PshAI GACNNNNGTC 1 cut(s) 284
Psp124BI GAGCTC 1 cut(s) 1074
Psp6I CCWGG 3 cut(s) 357, 934, 1287
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 3 cut(s) 357, 934, 1287
PspN4I GGNNCC 5 cut(s) 334, 375, 421, 729, 900
PspPI GGNCC 5 cut(s) 132, 187, 419, 898, 1122
PsuI RGATCY 2 cut(s) 727, 1116
PsyI GACNNNGTC 1 cut(s) 136
RsaI GTAC 1 cut(s) 1293
RsaNI GTAC 1 cut(s) 1292
SacI GAGCTC 1 cut(s) 1074
SaqAI TTAA 2 cut(s) 243, 741
SatI GCNGC 2 cut(s) 68, 1101
Sau3AI GATC 4 cut(s) 676, 727, 841, 1116
Sau96I GGNCC 5 cut(s) 132, 187, 419, 898, 1122
SchI GAGTC 2 cut(s) 295, 404
ScrFI CCNGG 3 cut(s) 359, 936, 1289
SduI GDGCHC 3 cut(s) 45, 1074, 1095
SfaNI GCATC 1 cut(s) 754
SfoI GGCGCC 1 cut(s) 334
Sfr274I CTCGAG 1 cut(s) 1073
SinI GGWCC 3 cut(s) 132, 419, 1122
SlaI CTCGAG 1 cut(s) 1073
SmlI CTYRAG 2 cut(s) 269, 1073
SmoI CTYRAG 2 cut(s) 269, 1073
SphI GCATGC 1 cut(s) 998
Sse9I AATT 7 cut(s) 293, 585, 669, 720, 777, 1214, 1305
SspDI GGCGCC 1 cut(s) 332
SspMI CTAG 1 cut(s) 1260
SstI GAGCTC 1 cut(s) 1074
StyD4I CCNGG 3 cut(s) 357, 934, 1287
StyI CCWWGG 1 cut(s) 771
TaaI ACNGT 3 cut(s) 91, 136, 283
TaiI ACGT 1 cut(s) 1287
TaqI TCGA 5 cut(s) 108, 352, 1008, 1074, 1089
TasI AATT 7 cut(s) 293, 585, 669, 720, 777, 1214, 1305
TfiI GAWTC 6 cut(s) 10, 101, 113, 472, 970, 1048
Tru1I TTAA 2 cut(s) 243, 741
Tru9I TTAA 2 cut(s) 243, 741
TscAI CASTG 2 cut(s) 559, 1208
TseFI GTSAC 1 cut(s) 925
TseI GCWGC 2 cut(s) 67, 1100
Tsp45I GTSAC 1 cut(s) 925
TspDTI ATGAA 3 cut(s) 165, 445, 977
TspRI CASTG 2 cut(s) 559, 1208
Tth111I GACNNNGTC 1 cut(s) 136
VpaK11BI GGWCC 3 cut(s) 132, 419, 1122
XapI RAATTY 2 cut(s) 720, 1214
XceI RCATGY 1 cut(s) 998
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XhoI CTCGAG 1 cut(s) 1073
XmiI GTMKAC 1 cut(s) 549
XmnI GAANNNNTTC 1 cut(s) 1254
XspI CTAG 1 cut(s) 1260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.