Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003158

Physical Location & Seq

Genomic Coordinates Reverse (-)

23794600 .. 23797593

Gene Structure 2994 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1144700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1635 bp

ATGGTTAAGGTTCGCGCTGGTGTTCTGCTGGTGCTCGCAGCTTGCTTTGCATTTCAGCAGCTATCTGCTTCCGACGATACGGTGTTTTACGACTCGTTTGATAACCCATTCGAAGGCCGATGGGTCGTTTCCAGCAAGGAAGAGTACCAAGGTGTGTGGAGCCACTCGAAGAGTGAGGGTCATGAGGATTACGGGCTTCTTGTGAGTGAGAAGGCCAAGAAGTACGCAATAGTGAAGGAGCTTGAGAAGCCTGAGAGTCTGAATGATGGAACTATTGTTCTCCAATTTGAGACTCGTCTCCAGAACGGGCTTGAATGTGGTGGTGCCTATCTGAAATACCTTCGACCCCAGGAGGCTGGATGGGAGCCTAAGATATTTGACAATGAGTCTCCTTATTCGATCATGTTTGGACCTGACAAATGTGGGCTCACAAACAAGGTTCACTTCATTTTCAAGCACAAGAACCCCAAGAGCGGAGAGTACGTTGAACACCATTTGACGACTCCACCCTCTGTCCCATCTGACAAACTCACTCATGTGTACACTGCCATTCTGAAACCAAATAACGAGTTGGTAATTCTGGTTGATGGAGAAGAGAAGAAGAAGGCGAATTTCCTATCAGCTGATGATTTTATGCCACCTCTTCTTCCTACCAAGACAATCCCTGATCCAGAGGATAAGAAGCCTGAGGACTGGGATGAGAGAGCCAAAATTCCAGACCCAAATGCCGTTAAGCCAGAGGATTGGGATGAGGACGCCCCAATGGAAATTGTTGATGAAGAAGCAGAGAAACCCGAAGGATGGTTGGATGATGAGCCTGAGGAGATAGATGATCCCGATGCCACAAAACCAGAAGATTGGGATGATGAGGAGGATGGTGAATGGGAAGCCCCAAGGATCGATAACCCGAAGTGTGTGACAGCCCCTGGTTGTGGCGAATGGAAGAAGCCAATGAAGAGAAACCCAGCTTACAAGGGAAAATGGCATGCTCCACTCATCGATAACCCCAGTTACAAGGGTATCTGGAAGCCTCAGGAGATTCCAAACCCCAGCTACTTTGAAGCTGACAAACCCAACTTTGAGTCTATCGCTGCTGTTGGCATTGAGATCTGGACAATGCAAGATGGTATATTGTTTGACAACATTTTGATAGCGAAAGACGAGAAAGTTGCACAAGCATACAGAGAGACCGCTTGGAAACCAAAATTTGAAGTTGAGAAGGAGAAGGCGAAGGAAGAGGAAGATGTTCAGGGCGCAGAAGGGTTCCTTGTTACTTTCCAGAAGAAAATATTTGACCTCCTATACACAGTCGCAGATCTTCCCTTTTTAAGTTCCCACAAGTCTAAGATCATTGATCTCATTGAGAAGGCAGAGCAACAACCAAATCTTACCGTTGGCATCCTTGTTGCCATTGTGGTGATCATCTTCACCGTATTCGTCAAGCTACTCTTTGGCGGGAAGAAACCTGCAAGAGTTGAGAAGAAAACCACCGGAGCCACCGCTGCTTCAAGCAGCAGCTCAGCAGCAGCTAAGGAAGATGAGAAGGAGAAGGAGAATGAAGGTACTTCATCTGAGAGCGAGAAGGAAGGAGCCGCTGCTCCTCGCAGAAGGACAACGACCAGGCGTGAAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005783 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0009507 GO:0009536 GO:0012505 GO:0016020 GO:0030054 GO:0030312 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04141 ko04145 ko04612 ko04918 ko05166 map04141 map04145 map04612 map04918 map05166

Pfam Domains

Protein Families

Calreticulin

Protein Analysis

545

Amino Acids

61.34

Weight (kDa)

4.83

Isoelectric Point (pI)

41.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Calreticulin	PF00262	29 - 384	1.5e-143	Calreticulin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003158)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07340 AT5G07340 AT5G61790
fragaria_vesca	FvH4_4g22170 FvH4_5g09940
malus_domestica	MD00G1076300.v1.1 MD08G1097600.v1.1 MD08G1097700.v1.1 MD13G1134200.v1.1 MD14G1144700.v1.1 MD16G1144800.v1.1
prunus_persica	Prupe.1G211400_v2.0.a1 Prupe.5G139100_v2.0.a1 Prupe.5G139100_v2.0.a1
pyrus_communis	pycom06g11940 pycom13g11700 pycom14g12020 pycom16g11430
rosa_chinensis	RchiOBHm_Chr4g0428401 RchiOBHm_Chr7g0188351
rosa_laevigata	RLG00000004706 RLG00000007154
rosa_multiflora	Rmu_sc0004698.1_g000004 Rmu_sc0008750.1_g000004
rosa_roxburghii	Rroxscaffold_3G00266480 Rroxscaffold_5G00370070
rosa_rugosa	Rorug04G0225200 Rorug06G0490000 Rorug06G0490100
rosa_samantha	Rh4AG282400 Rh4BG288000 Rh4CG303600 Rh4DG285100 Rh7BG096200 Rh7CG096500
rosa_wichuraiana	Rw4G024510 Rw7G008160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1474
AccB1I	GGYRCC	1 cut(s)	323
AccBSI	CCGCTC	1 cut(s)	474
AccII	CGCG	1 cut(s)	15
AciI	CCGC	5 cut(s)	474, 1191, 1455, 1500, 1593
AclWI	GGATC	3 cut(s)	662, 827, 905
AcsI	RAATTY	3 cut(s)	610, 711, 1205
AcyI	GRCGYC	1 cut(s)	756
AfaI	GTAC	5 cut(s)	146, 224, 482, 542, 1564
AfiI	CCNNNNNNNGG	4 cut(s)	113, 473, 801, 932
AgsI	TTSAA	6 cut(s)	314, 454, 488, 1061, 1211, 1509
AhdI	GACNNNNNGTC	1 cut(s)	385
AjnI	CCWGG	3 cut(s)	348, 925, 1619
AleI	CACNNNNGTG	1 cut(s)	536
AloI	GAACNNNNNNTCC	2 cut(s)	261, 293
Alw21I	GWGCWC	1 cut(s)	36
Alw26I	GTCTC	4 cut(s)	284, 302, 393, 1181
AlwI	GGATC	3 cut(s)	662, 827, 905
AlwNI	CAGNNNCTG	1 cut(s)	926
AoxI	GGCC	2 cut(s)	115, 213
ApeKI	GCWGC	9 cut(s)	38, 58, 1091, 1502, 1512, 1515, 1523, 1526, 1595
ApoI	RAATTY	3 cut(s)	610, 711, 1205
Asp700I	GAANNNNTTC	1 cut(s)	1245
AspLEI	GCGC	2 cut(s)	17, 1256
AspS9I	GGNCC	1 cut(s)	410
AsuHPI	GGTGA	3 cut(s)	890, 1420, 1429
AsuII	TTCGAA	1 cut(s)	111
AvaII	GGWCC	1 cut(s)	410
AxyI	CCTNAGG	3 cut(s)	687, 819, 1032
BaeI	ACNNNNGTAYC	2 cut(s)	1003, 1036
BanI	GGYRCC	1 cut(s)	323
BanII	GRGCYC	1 cut(s)	429
Bbv12I	GWGCWC	1 cut(s)	36
BbvI	GCAGC	9 cut(s)	50, 70, 1078, 1489, 1524, 1527, 1535, 1538, 1582
BccI	CCATC	8 cut(s)	114, 260, 354, 526, 581, 795, 869, 1118
BceAI	ACGGC	1 cut(s)	713
BcgI	CGANNNNNNTGC	2 cut(s)	1151, 1185
BciT130I	CCWGG	3 cut(s)	350, 927, 1621
BclI	TGATCA	1 cut(s)	1419
BcoDI	GTCTC	4 cut(s)	284, 302, 393, 1181
BfuAI	ACCTGC	1 cut(s)	1474
BglII	AGATCT	2 cut(s)	1107, 1315
BlpI	GCTNAGC	1 cut(s)	1519
Bme1390I	CCNGG	3 cut(s)	350, 927, 1621
Bme18I	GGWCC	1 cut(s)	410
BmeRI	GACNNNNNGTC	1 cut(s)	385
BmgT120I	GGNCC	1 cut(s)	410
BmiI	GGNNCC	6 cut(s)	161, 325, 366, 1265, 1495, 1591
BmrFI	CCNGG	3 cut(s)	350, 927, 1621
BmrI	ACTGGG	2 cut(s)	703, 1002
BmsI	GCATC	2 cut(s)	829, 1407
BmuI	ACTGGG	2 cut(s)	703, 1002
BpmI	CTGGAG	1 cut(s)	284
Bpu10I	CCTNAGC	1 cut(s)	1530
Bpu1102I	GCTNAGC	1 cut(s)	1519
Bpu14I	TTCGAA	1 cut(s)	111
BpuEI	CTTGAG	1 cut(s)	263
Bsa29I	ATCGAT	2 cut(s)	900, 999
BsaHI	GRCGYC	1 cut(s)	756
BsaI	GGTCTC	1 cut(s)	1181
BsaJI	CCNNGG	4 cut(s)	148, 348, 893, 925
BsaWI	WCCGGW	1 cut(s)	1490
BsaXI	ACNNNNNCTCC	2 cut(s)	468, 498
Bsc4I	CCNNNNNNNGG	4 cut(s)	113, 473, 801, 932
Bse1I	ACTGG	2 cut(s)	698, 1008
Bse21I	CCTNAGG	3 cut(s)	687, 819, 1032
BseBI	CCWGG	3 cut(s)	350, 927, 1621
BseCI	ATCGAT	2 cut(s)	900, 999
BseDI	CCNNGG	4 cut(s)	148, 348, 893, 925
BseGI	GGATG	8 cut(s)	365, 703, 754, 806, 814, 868, 880, 1398
BseLI	CCNNNNNNNGG	4 cut(s)	113, 473, 801, 932
BseMII	CTCAG	6 cut(s)	243, 678, 810, 1046, 1533, 1563
BseNI	ACTGG	2 cut(s)	698, 1008
BseRI	GAGGAG	3 cut(s)	836, 884, 1590
BseXI	GCAGC	9 cut(s)	50, 70, 1078, 1489, 1524, 1527, 1535, 1538, 1582
BseYI	CCCAGC	2 cut(s)	964, 1049
Bsh1236I	CGCG	1 cut(s)	15
BshFI	GGCC	2 cut(s)	117, 215
BshNI	GGYRCC	1 cut(s)	323
BshVI	ATCGAT	2 cut(s)	900, 999
BsiHKAI	GWGCWC	1 cut(s)	36
BsiSI	CCGG	1 cut(s)	1491
BslFI	GGGAC	1 cut(s)	500
BslI	CCNNNNNNNGG	4 cut(s)	113, 473, 801, 932
BsmAI	GTCTC	4 cut(s)	284, 302, 393, 1181
BsmBI	CGTCTC	1 cut(s)	302
BsmFI	GGGAC	1 cut(s)	500
BsnI	GGCC	2 cut(s)	117, 215
Bso31I	GGTCTC	1 cut(s)	1181
Bsp119I	TTCGAA	1 cut(s)	111
Bsp1286I	GDGCHC	2 cut(s)	36, 429
Bsp1407I	TGTACA	1 cut(s)	540
Bsp143I	GATC	9 cut(s)	399, 667, 832, 897, 1107, 1315, 1347, 1354, 1419
Bsp1720I	GCTNAGC	1 cut(s)	1519
BspACI	CCGC	5 cut(s)	474, 1191, 1455, 1500, 1593
BspANI	GGCC	2 cut(s)	117, 215
BspCNI	CTCAG	6 cut(s)	244, 679, 811, 1045, 1532, 1564
BspDI	ATCGAT	2 cut(s)	900, 999
BspFNI	CGCG	1 cut(s)	15
BspHI	TCATGA	1 cut(s)	181
BspLI	GGNNCC	6 cut(s)	161, 325, 366, 1265, 1495, 1591
BspMI	ACCTGC	1 cut(s)	1474
BspPI	GGATC	3 cut(s)	662, 827, 905
BspT104I	TTCGAA	1 cut(s)	111
BspT107I	GGYRCC	1 cut(s)	323
BspTNI	GGTCTC	1 cut(s)	1181
BsrBI	CCGCTC	1 cut(s)	474
BsrGI	TGTACA	1 cut(s)	540
BsrI	ACTGG	2 cut(s)	698, 1008
BssECI	CCNNGG	4 cut(s)	148, 348, 893, 925
BssMI	GATC	9 cut(s)	399, 667, 832, 897, 1107, 1315, 1347, 1354, 1419
BssNI	GRCGYC	1 cut(s)	756
BssT1I	CCWWGG	2 cut(s)	148, 893
Bst2UI	CCWGG	3 cut(s)	350, 927, 1621
Bst4CI	ACNGT	4 cut(s)	82, 1309, 1393, 1432
Bst6I	CTCTTC	6 cut(s)	135, 164, 588, 648, 950, 1230
BstACI	GRCGYC	1 cut(s)	756
BstAUI	TGTACA	1 cut(s)	540
BstBI	TTCGAA	1 cut(s)	111
BstC8I	GCNNGC	3 cut(s)	36, 43, 987
BstDEI	CTNAG	9 cut(s)	252, 369, 687, 819, 1032, 1344, 1519, 1530, 1572
BstF5I	GGATG	8 cut(s)	365, 703, 754, 806, 814, 868, 880, 1398
BstFNI	CGCG	1 cut(s)	15
BstHHI	GCGC	2 cut(s)	17, 1256
BstKTI	GATC	9 cut(s)	402, 670, 835, 900, 1110, 1318, 1350, 1357, 1422
BstMAI	GTCTC	4 cut(s)	284, 302, 393, 1181
BstMBI	GATC	9 cut(s)	399, 667, 832, 897, 1107, 1315, 1347, 1354, 1419
BstMWI	GCNNNNNNNGC	3 cut(s)	47, 247, 1502
BstNI	CCWGG	3 cut(s)	350, 927, 1621
BstNSI	RCATGY	1 cut(s)	989
BstSCI	CCNGG	3 cut(s)	348, 925, 1619
BstUI	CGCG	1 cut(s)	15
BstV1I	GCAGC	9 cut(s)	50, 70, 1078, 1489, 1524, 1527, 1535, 1538, 1582
BstX2I	RGATCY	2 cut(s)	1107, 1315
BstXI	CCANNNNNNTGG	3 cut(s)	356, 744, 858
BstYI	RGATCY	2 cut(s)	1107, 1315
Bsu15I	ATCGAT	2 cut(s)	900, 999
Bsu36I	CCTNAGG	3 cut(s)	687, 819, 1032
BsuRI	GGCC	2 cut(s)	117, 215
BsuTUI	ATCGAT	2 cut(s)	900, 999
BtsCI	GGATG	8 cut(s)	365, 703, 754, 806, 814, 868, 880, 1398
BtsI	GCAGTG	1 cut(s)	543
BtsIMutI	CAGTG	1 cut(s)	543
BveI	ACCTGC	1 cut(s)	1474
Cac8I	GCNNGC	3 cut(s)	36, 43, 987
CaiI	CAGNNNCTG	1 cut(s)	926
CciI	TCATGA	1 cut(s)	181
CfoI	GCGC	2 cut(s)	17, 1256
Cfr13I	GGNCC	1 cut(s)	410
ClaI	ATCGAT	2 cut(s)	900, 999
CseI	GACGC	1 cut(s)	764
Csp6I	GTAC	5 cut(s)	145, 223, 481, 541, 1563
CspCI	CAANNNNNGTGG	2 cut(s)	137, 172
CviAII	CATG	4 cut(s)	182, 403, 536, 986
CviQI	GTAC	5 cut(s)	145, 223, 481, 541, 1563
DdeI	CTNAG	9 cut(s)	252, 369, 687, 819, 1032, 1344, 1519, 1530, 1572
DpnI	GATC	9 cut(s)	401, 669, 834, 899, 1109, 1317, 1349, 1356, 1421
DpnII	GATC	9 cut(s)	399, 667, 832, 897, 1107, 1315, 1347, 1354, 1419
DriI	GACNNNNNGTC	1 cut(s)	385
Eam1104I	CTCTTC	6 cut(s)	135, 164, 588, 648, 950, 1230
Eam1105I	GACNNNNNGTC	1 cut(s)	385
EarI	CTCTTC	6 cut(s)	135, 164, 588, 648, 950, 1230
Eco130I	CCWWGG	2 cut(s)	148, 893
Eco24I	GRGCYC	1 cut(s)	429
Eco31I	GGTCTC	1 cut(s)	1181
Eco47I	GGWCC	1 cut(s)	410
Eco81I	CCTNAGG	3 cut(s)	687, 819, 1032
EcoRII	CCWGG	3 cut(s)	348, 925, 1619
EcoT14I	CCWWGG	2 cut(s)	148, 893
EcoT38I	GRGCYC	1 cut(s)	429
ErhI	CCWWGG	2 cut(s)	148, 893
Esp3I	CGTCTC	1 cut(s)	302
FaeI	CATG	4 cut(s)	185, 406, 539, 989
FaiI	YATR	8 cut(s)	183, 404, 537, 635, 987, 1130, 1180, 1303
FalI	AAGNNNNNCTT	6 cut(s)	428, 460, 1251, 1283, 1433, 1465
FaqI	GGGAC	1 cut(s)	500
FatI	CATG	4 cut(s)	181, 402, 535, 985
FauI	CCCGC	1 cut(s)	1448
FbaI	TGATCA	1 cut(s)	1419
FokI	GGATG	8 cut(s)	372, 710, 761, 813, 821, 875, 887, 1385
FriOI	GRGCYC	1 cut(s)	429
GlaI	GCGC	2 cut(s)	16, 1255
GsaI	CCCAGC	2 cut(s)	968, 1053
GsuI	CTGGAG	1 cut(s)	284
HaeIII	GGCC	2 cut(s)	117, 215
HapII	CCGG	1 cut(s)	1491
HgaI	GACGC	1 cut(s)	764
HhaI	GCGC	2 cut(s)	17, 1256
Hin1I	GRCGYC	1 cut(s)	756
Hin1II	CATG	4 cut(s)	185, 406, 539, 989
Hin6I	GCGC	2 cut(s)	15, 1254
HinP1I	GCGC	2 cut(s)	15, 1254
HinfI	GANTC	7 cut(s)	92, 256, 292, 386, 502, 1039, 1082
HpaII	CCGG	1 cut(s)	1491
HphI	GGTGA	3 cut(s)	890, 1420, 1429
Hpy166II	GTNNAC	3 cut(s)	442, 541, 543
Hpy188I	TCNGA	6 cut(s)	73, 261, 333, 523, 555, 1573
Hpy188III	TCNNGA	9 cut(s)	182, 301, 671, 716, 836, 1024, 1034, 1111, 1279
Hpy8I	GTNNAC	3 cut(s)	442, 541, 543
Hpy99I	CGWCG	1 cut(s)	77
HpyCH4III	ACNGT	4 cut(s)	82, 1309, 1393, 1432
HpyCH4IV	ACGT	1 cut(s)	483
HpyCH4V	TGCA	4 cut(s)	50, 1120, 1172, 1469
HpyF10VI	GCNNNNNNNGC	3 cut(s)	47, 247, 1502
HpyF3I	CTNAG	9 cut(s)	252, 369, 687, 819, 1032, 1344, 1519, 1530, 1572
HpySE526I	ACGT	1 cut(s)	483
Hsp92I	GRCGYC	1 cut(s)	756
Hsp92II	CATG	4 cut(s)	185, 406, 539, 989
HspAI	GCGC	2 cut(s)	15, 1254
Ksp22I	TGATCA	1 cut(s)	1419
Kzo9I	GATC	9 cut(s)	399, 667, 832, 897, 1107, 1315, 1347, 1354, 1419
LmnI	GCTCC	7 cut(s)	159, 238, 364, 994, 1493, 1589, 1603
Lsp1109I	GCAGC	9 cut(s)	50, 70, 1078, 1489, 1524, 1527, 1535, 1538, 1582
LweI	GCATC	2 cut(s)	829, 1407
MaeII	ACGT	1 cut(s)	483
MaeIII	GTNAC	3 cut(s)	916, 1010, 1270
MalI	GATC	9 cut(s)	401, 669, 834, 899, 1109, 1317, 1349, 1356, 1421
MbiI	CCGCTC	1 cut(s)	474
MboI	GATC	9 cut(s)	399, 667, 832, 897, 1107, 1315, 1347, 1354, 1419
MflI	RGATCY	2 cut(s)	1107, 1315
MhlI	GDGCHC	2 cut(s)	36, 429
MluCI	AATT	6 cut(s)	284, 576, 610, 711, 768, 1205
MlyI	GAGTC	6 cut(s)	86, 265, 286, 395, 496, 1091
MmeI	TCCRAC	2 cut(s)	96, 786
MroXI	GAANNNNTTC	1 cut(s)	1245
MseI	TTAA	3 cut(s)	6, 732, 1328
MslI	CAYNNNNRTG	2 cut(s)	536, 1415
MspA1I	CMGCKG	3 cut(s)	623, 1502, 1595
MspI	CCGG	1 cut(s)	1491
MspR9I	CCNGG	3 cut(s)	350, 927, 1621
MvaI	CCWGG	3 cut(s)	350, 927, 1621
MvnI	CGCG	1 cut(s)	15
MwoI	GCNNNNNNNGC	3 cut(s)	47, 247, 1502
NdeII	GATC	9 cut(s)	399, 667, 832, 897, 1107, 1315, 1347, 1354, 1419
NlaIII	CATG	4 cut(s)	185, 406, 539, 989
NlaIV	GGNNCC	6 cut(s)	161, 325, 366, 1265, 1495, 1591
NmuCI	GTSAC	1 cut(s)	916
NspI	RCATGY	1 cut(s)	989
NspV	TTCGAA	1 cut(s)	111
OliI	CACNNNNGTG	1 cut(s)	536
PaeI	GCATGC	1 cut(s)	989
PagI	TCATGA	1 cut(s)	181
PdmI	GAANNNNTTC	1 cut(s)	1245
PfeI	GAWTC	1 cut(s)	1039
PleI	GAGTC	6 cut(s)	86, 264, 286, 394, 496, 1090
PpsI	GAGTC	6 cut(s)	86, 264, 286, 394, 496, 1090
Psp6I	CCWGG	3 cut(s)	348, 925, 1619
PspFI	CCCAGC	2 cut(s)	964, 1049
PspGI	CCWGG	3 cut(s)	348, 925, 1619
PspN4I	GGNNCC	6 cut(s)	161, 325, 366, 1265, 1495, 1591
PspPI	GGNCC	1 cut(s)	410
PstNI	CAGNNNCTG	1 cut(s)	926
PsuI	RGATCY	2 cut(s)	1107, 1315
PvuII	CAGCTG	1 cut(s)	623
RsaI	GTAC	5 cut(s)	146, 224, 482, 542, 1564
RsaNI	GTAC	5 cut(s)	145, 223, 481, 541, 1563
RseI	CAYNNNNRTG	2 cut(s)	536, 1415
SaqAI	TTAA	3 cut(s)	6, 732, 1328
Sau3AI	GATC	9 cut(s)	399, 667, 832, 897, 1107, 1315, 1347, 1354, 1419
Sau96I	GGNCC	1 cut(s)	410
SchI	GAGTC	6 cut(s)	86, 265, 286, 395, 496, 1091
ScrFI	CCNGG	3 cut(s)	350, 927, 1621
SduI	GDGCHC	2 cut(s)	36, 429
SfaNI	GCATC	2 cut(s)	829, 1407
SfuI	TTCGAA	1 cut(s)	111
SinI	GGWCC	1 cut(s)	410
SmiMI	CAYNNNNRTG	2 cut(s)	536, 1415
SmlI	CTYRAG	1 cut(s)	242
SmoI	CTYRAG	1 cut(s)	242
SphI	GCATGC	1 cut(s)	989
Sse9I	AATT	6 cut(s)	284, 576, 610, 711, 768, 1205
SsiI	CCGC	5 cut(s)	474, 1191, 1455, 1500, 1593
SspI	AATATT	1 cut(s)	1290
StyD4I	CCNGG	3 cut(s)	348, 925, 1619
StyI	CCWWGG	2 cut(s)	148, 893
TaaI	ACNGT	4 cut(s)	82, 1309, 1393, 1432
TaiI	ACGT	1 cut(s)	486
TaqI	TCGA	6 cut(s)	111, 167, 343, 398, 900, 999
TasI	AATT	6 cut(s)	284, 576, 610, 711, 768, 1205
TatI	WGTACW	1 cut(s)	540
TauI	GCSGC	1 cut(s)	1595
TfiI	GAWTC	1 cut(s)	1039
Tru1I	TTAA	3 cut(s)	6, 732, 1328
Tru9I	TTAA	3 cut(s)	6, 732, 1328
TscAI	CASTG	1 cut(s)	550
TseFI	GTSAC	1 cut(s)	916
TseI	GCWGC	9 cut(s)	38, 58, 1091, 1502, 1512, 1515, 1523, 1526, 1595
Tsp45I	GTSAC	1 cut(s)	916
TspDTI	ATGAA	5 cut(s)	436, 792, 968, 1557, 1572
TspRI	CASTG	1 cut(s)	550
VpaK11BI	GGWCC	1 cut(s)	410
XapI	RAATTY	3 cut(s)	610, 711, 1205
XceI	RCATGY	1 cut(s)	989
XmnI	GAANNNNTTC	1 cut(s)	1245

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.