Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003158

Physical Location & Seq

Genomic Coordinates Forward (+)

10274662 .. 10278695

Gene Structure 4034 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1134200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1644 bp

ATGGCAGGAACACGGTGGCGTCGAATCACTGACCTCCTTCTTCTTCTGCTGCTGATTGGCTGCTCTGCTTCCCAGATCCACGCTTCTTCAGAATACGAAGTCGATTCGATCTTTTACGAGCCATTTTCTGAGTCGTTTGAAGGCAGATGGATTGTTTCTTCGAAAGATGAGTACCAAGGGGTATGGAAACTGTCCAAGAGTGAGGGACATGATGATTACGGGCTTCTGGTAAGTGAGAAGGCGAGGAAGTATGCAGTGGTGAAAGAGATGGACAAGGCTGTGAGCCTTGCTGATGGAACTGTTGTCCTACAGTTTGAGGTCCGGCTCCAGAACGGGCTTGAATGTGGCGGTGCTTACTTGAAGTACCTCCGGCCACAGGAGGCTGGATGGAAAGCGGAGGAATTTGACAATGAGTCGCCATATTCTATTATGTTTGGACCGGACAAATGTGGTTCCACAAACAAGGTTCATTTCATTTTTAAGCACAAGAACCCCAAGAGTGGAGAGTATGTTGAGCACCATCTCAAGGACCCTCCATCCGTGCCCTCCGATAAGCTGTCTCATGTTTACACTGCCATACTGAAACCTGATAACGAAGTTAGGATTTTGATAGATGGGGAGGAGAAGAAGAAGGCGAATTTCCTGTCGAATAAAGATTTTGAGCCTGCATTGATTCCAGCCAAGACAATTCCTGACCCAGAGGATCAAAAGCCAGAGGATTGGGATGAGCGGCTGAAGATTCCAGACCCCAATGCCGTTAAGCCTGATGATTGGGACGAGGATGCACCAATGGAAATTGAGGACGAGGATGCTGTGAAACCTGAAGGGTGGTTGGATGATGAGCCTGAAGAGATTGATGACCCTGAGGCGACAAGGCCAGAAGATTGGGACGATGAAGAGGATGGTGTATGGGAACCGACAAAGACGGATAACCCCAAGTGTTCATTGGCACCTGGTTGTGGAGAATGGAAGAAGCCAATGAAACATAATCCTGCTTATAAAGGAAAATGGAGCCCTCCTATGATTGACAACCCCAGTTACAAGGGCATCTGGAAGCCTCGAGAAATTCCAAACCCTGATTACTTCGAGACTGAGAAGCCCAACTTTGAGCCCATTGCTGCTATTGGCATTGAGATCTGGACAATGCAGGACGGAATATTGTTTGACAATATGCTCATAGCTGATGATGAGAAGGTTGCTGAATCTGTTAGGCTAACAACGTGGAAGCCGAAGTTTGAAACTGAGAAAGAGAAACAGAAGGCTGAGGAAGAAGCTGCAGGTCTTTCCGATGGACTCTCAAGCTACCAGAAGAAAGTATTCGACCTTCTTTACAAGATAGCAGATGTTTCTTTCTTGGATGCATACAAACCCAGGATCATTGAATTGATTGAGAAGGGAGAAAAACAGCCCAATATTACAATTGGTGTTATCGTCTCTGTCGTGGTTGTTCTCTTGTCAATTTTCTTTAAAATCCTGTTTGGTGGCAAGAAACCGGCGGTGGTCACTGTTCCAGAACCAAGCAGGGCTGCGGCTGCACGGACTTCCCATGATGAAGGAAGCAGCGGCGAGAAGGAAGATGAGATTGAGAAAGAAGAAGCTGCTGCTCCTCCTCCCCGCAGGCGGTCTGCCAGAAGGGAAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005783 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0009507 GO:0009536 GO:0012505 GO:0016020 GO:0030054 GO:0030312 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04141 ko04145 ko04612 ko04918 ko05166 map04141 map04145 map04612 map04918 map05166

Pfam Domains

Protein Families

Calreticulin

Protein Analysis

548

Amino Acids

61.97

Weight (kDa)

4.78

Isoelectric Point (pI)

46.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Calreticulin	PF00262	38 - 393	4.5e-144	Calreticulin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003158)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07340 AT5G07340 AT5G61790
fragaria_vesca	FvH4_4g22170 FvH4_5g09940
malus_domestica	MD00G1076300.v1.1 MD08G1097600.v1.1 MD08G1097700.v1.1 MD13G1134200.v1.1 MD14G1144700.v1.1 MD16G1144800.v1.1
prunus_persica	Prupe.1G211400_v2.0.a1 Prupe.5G139100_v2.0.a1 Prupe.5G139100_v2.0.a1
pyrus_communis	pycom06g11940 pycom13g11700 pycom14g12020 pycom16g11430
rosa_chinensis	RchiOBHm_Chr4g0428401 RchiOBHm_Chr7g0188351
rosa_laevigata	RLG00000004706 RLG00000007154
rosa_multiflora	Rmu_sc0004698.1_g000004 Rmu_sc0008750.1_g000004
rosa_roxburghii	Rroxscaffold_3G00266480 Rroxscaffold_5G00370070
rosa_rugosa	Rorug04G0225200 Rorug06G0490000 Rorug06G0490100
rosa_samantha	Rh4AG282400 Rh4BG288000 Rh4CG303600 Rh4DG285100 Rh7BG096200 Rh7CG096500
rosa_wichuraiana	Rw4G024510 Rw7G008160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	999
Acc36I	ACCTGC	1 cut(s)	1268
AccB1I	GGYRCC	1 cut(s)	949
AccBSI	CCGCTC	1 cut(s)	730
AciI	CCGC	8 cut(s)	348, 395, 730, 1496, 1529, 1563, 1615, 1621
AclWI	GGATC	3 cut(s)	70, 711, 1382
AcoI	YGGCCR	1 cut(s)	371
AcsI	RAATTY	3 cut(s)	401, 637, 1065
AcuI	CTGAAG	4 cut(s)	72, 755, 843, 867
AcyI	GRCGYC	1 cut(s)	19
AfaI	GTAC	2 cut(s)	173, 365
AfiI	CCNNNNNNNGG	5 cut(s)	376, 500, 526, 959, 1620
AgsI	TTSAA	5 cut(s)	140, 341, 361, 1238, 1382
AhdI	GACNNNNNGTC	1 cut(s)	412
AjnI	CCWGG	2 cut(s)	952, 1370
AluBI	AGCT	5 cut(s)	556, 1181, 1274, 1302, 1598
AluI	AGCT	5 cut(s)	556, 1181, 1274, 1302, 1598
Alw21I	GWGCWC	1 cut(s)	519
Alw26I	GTCTC	3 cut(s)	564, 1082, 1438
AlwI	GGATC	3 cut(s)	70, 711, 1382
Ama87I	CYCGRG	1 cut(s)	1059
AoxI	GGCC	2 cut(s)	371, 875
ApeKI	GCWGC	9 cut(s)	49, 60, 1118, 1274, 1526, 1532, 1560, 1598, 1601
ApoI	RAATTY	3 cut(s)	401, 637, 1065
ArsI	GACNNNNNNTTYG	2 cut(s)	629, 661
Asp700I	GAANNNNTTC	1 cut(s)	1316
AspS9I	GGNCC	3 cut(s)	319, 437, 529
AsuHPI	GGTGA	1 cut(s)	271
AsuII	TTCGAA	1 cut(s)	161
AvaI	CYCGRG	1 cut(s)	1059
AvaII	GGWCC	3 cut(s)	319, 437, 529
AxyI	CCTNAGG	1 cut(s)	864
BaeGI	GKGCMC	1 cut(s)	546
BanI	GGYRCC	1 cut(s)	949
BanII	GRGCYC	2 cut(s)	1016, 1113
BarI	GAAGNNNNNNTAC	2 cut(s)	1308, 1340
Bbv12I	GWGCWC	1 cut(s)	519
BbvCI	CCTCAGC	1 cut(s)	1263
BbvI	GCAGC	9 cut(s)	36, 47, 1105, 1261, 1513, 1519, 1572, 1585, 1588
BccI	CCATC	9 cut(s)	141, 262, 287, 381, 528, 544, 608, 896, 1283
BceAI	ACGGC	1 cut(s)	740
BciT130I	CCWGG	2 cut(s)	954, 1372
BcoDI	GTCTC	3 cut(s)	564, 1082, 1438
BfmI	CTRYAG	2 cut(s)	308, 1275
BfuAI	ACCTGC	1 cut(s)	1268
BglII	AGATCT	1 cut(s)	1134
Bme1390I	CCNGG	2 cut(s)	954, 1372
Bme18I	GGWCC	3 cut(s)	319, 437, 529
BmeRI	GACNNNNNGTC	1 cut(s)	412
BmeT110I	CYCGRG	1 cut(s)	1059
BmgT120I	GGNCC	3 cut(s)	319, 437, 529
BmiI	GGNNCC	6 cut(s)	326, 454, 531, 915, 951, 1013
BmrFI	CCNGG	2 cut(s)	954, 1372
BmrI	ACTGGG	1 cut(s)	1029
BmsI	GCATC	4 cut(s)	772, 799, 1056, 1348
BmuI	ACTGGG	1 cut(s)	1029
BpmI	CTGGAG	1 cut(s)	311
Bpu10I	CCTNAGC	1 cut(s)	1263
Bpu14I	TTCGAA	1 cut(s)	161
BpuEI	CTTGAG	2 cut(s)	509, 1282
BsaHI	GRCGYC	1 cut(s)	19
BsaJI	CCNNGG	2 cut(s)	175, 1370
BsaWI	WCCGGW	1 cut(s)	439
BsaXI	ACNNNNNCTCC	2 cut(s)	495, 525
Bsc4I	CCNNNNNNNGG	5 cut(s)	376, 500, 526, 959, 1620
Bse118I	RCCGGY	1 cut(s)	1492
Bse1I	ACTGG	1 cut(s)	1035
Bse21I	CCTNAGG	1 cut(s)	864
Bse3DI	GCAATG	1 cut(s)	1113
BseBI	CCWGG	2 cut(s)	954, 1372
BseDI	CCNNGG	2 cut(s)	175, 1370
BseGI	GGATG	8 cut(s)	392, 536, 730, 787, 814, 841, 907, 1363
BseLI	CCNNNNNNNGG	5 cut(s)	376, 500, 526, 959, 1620
BseMI	GCAATG	1 cut(s)	1113
BseMII	CTCAG	5 cut(s)	120, 855, 1083, 1233, 1254
BseNI	ACTGG	1 cut(s)	1035
BseRI	GAGGAG	3 cut(s)	635, 1596, 1599
BseSI	GKGCMC	1 cut(s)	546
BseXI	GCAGC	9 cut(s)	36, 47, 1105, 1261, 1513, 1519, 1572, 1585, 1588
BsgI	GTGCAG	1 cut(s)	1518
BshFI	GGCC	2 cut(s)	373, 877
BshNI	GGYRCC	1 cut(s)	949
BsiHKAI	GWGCWC	1 cut(s)	519
BsiHKCI	CYCGRG	1 cut(s)	1059
BsiSI	CCGG	4 cut(s)	322, 370, 440, 1493
BslFI	GGGAC	3 cut(s)	219, 788, 902
BslI	CCNNNNNNNGG	5 cut(s)	376, 500, 526, 959, 1620
BsmAI	GTCTC	3 cut(s)	564, 1082, 1438
BsmBI	CGTCTC	1 cut(s)	1438
BsmFI	GGGAC	3 cut(s)	219, 788, 902
BsnI	GGCC	2 cut(s)	373, 877
BsoBI	CYCGRG	1 cut(s)	1059
Bsp119I	TTCGAA	1 cut(s)	161
Bsp1286I	GDGCHC	4 cut(s)	519, 546, 1016, 1113
Bsp143I	GATC	5 cut(s)	75, 108, 703, 1134, 1374
BspACI	CCGC	8 cut(s)	348, 395, 730, 1496, 1529, 1563, 1615, 1621
BspANI	GGCC	2 cut(s)	373, 877
BspCNI	CTCAG	5 cut(s)	121, 856, 1084, 1234, 1255
BspLI	GGNNCC	6 cut(s)	326, 454, 531, 915, 951, 1013
BspMAI	CTGCAG	1 cut(s)	1279
BspMI	ACCTGC	1 cut(s)	1268
BspPI	GGATC	3 cut(s)	70, 711, 1382
BspT104I	TTCGAA	1 cut(s)	161
BspT107I	GGYRCC	1 cut(s)	949
BsrBI	CCGCTC	1 cut(s)	730
BsrDI	GCAATG	1 cut(s)	1113
BsrFI	RCCGGY	1 cut(s)	1492
BsrI	ACTGG	1 cut(s)	1035
BssAI	RCCGGY	1 cut(s)	1492
BssECI	CCNNGG	2 cut(s)	175, 1370
BssMI	GATC	5 cut(s)	75, 108, 703, 1134, 1374
BssNI	GRCGYC	1 cut(s)	19
BssT1I	CCWWGG	1 cut(s)	175
Bst2UI	CCWGG	2 cut(s)	954, 1372
Bst4CI	ACNGT	5 cut(s)	15, 192, 301, 312, 1507
Bst6I	CTCTTC	2 cut(s)	843, 891
BstACI	GRCGYC	1 cut(s)	19
BstBI	TTCGAA	1 cut(s)	161
BstC8I	GCNNGC	2 cut(s)	666, 1619
BstDEI	CTNAG	5 cut(s)	129, 864, 1092, 1242, 1263
BstF5I	GGATG	8 cut(s)	392, 536, 730, 787, 814, 841, 907, 1363
BstKTI	GATC	5 cut(s)	78, 111, 706, 1137, 1377
BstMAI	GTCTC	3 cut(s)	564, 1082, 1438
BstMBI	GATC	5 cut(s)	75, 108, 703, 1134, 1374
BstMWI	GCNNNNNNNGC	1 cut(s)	1532
BstNI	CCWGG	2 cut(s)	954, 1372
BstSCI	CCNGG	2 cut(s)	952, 1370
BstSFI	CTRYAG	2 cut(s)	308, 1275
BstSLI	GKGCMC	1 cut(s)	546
BstV1I	GCAGC	9 cut(s)	36, 47, 1105, 1261, 1513, 1519, 1572, 1585, 1588
BstX2I	RGATCY	2 cut(s)	75, 1134
BstXI	CCANNNNNNTGG	2 cut(s)	720, 885
BstYI	RGATCY	2 cut(s)	75, 1134
Bsu36I	CCTNAGG	1 cut(s)	864
BsuRI	GGCC	2 cut(s)	373, 877
BtsCI	GGATG	8 cut(s)	392, 536, 730, 787, 814, 841, 907, 1363
BtsI	GCAGTG	2 cut(s)	261, 570
BtsIMutI	CAGTG	4 cut(s)	27, 261, 570, 1503
BveI	ACCTGC	1 cut(s)	1268
Cac8I	GCNNGC	2 cut(s)	666, 1619
Cfr10I	RCCGGY	1 cut(s)	1492
Cfr13I	GGNCC	3 cut(s)	319, 437, 529
CseI	GACGC	1 cut(s)	8
CsiI	ACCWGGT	1 cut(s)	952
Csp6I	GTAC	2 cut(s)	172, 364
CviAII	CATG	4 cut(s)	209, 563, 1547, 1641
CviQI	GTAC	2 cut(s)	172, 364
DdeI	CTNAG	5 cut(s)	129, 864, 1092, 1242, 1263
DpnI	GATC	5 cut(s)	77, 110, 705, 1136, 1376
DpnII	GATC	5 cut(s)	75, 108, 703, 1134, 1374
DraI	TTTAAA	1 cut(s)	1468
DriI	GACNNNNNGTC	1 cut(s)	412
EaeI	YGGCCR	1 cut(s)	371
Eam1104I	CTCTTC	2 cut(s)	843, 891
Eam1105I	GACNNNNNGTC	1 cut(s)	412
EarI	CTCTTC	2 cut(s)	843, 891
Eco130I	CCWWGG	1 cut(s)	175
Eco24I	GRGCYC	2 cut(s)	1016, 1113
Eco47I	GGWCC	3 cut(s)	319, 437, 529
Eco57I	CTGAAG	4 cut(s)	72, 755, 843, 867
Eco81I	CCTNAGG	1 cut(s)	864
Eco88I	CYCGRG	1 cut(s)	1059
EcoO109I	RGGNCCY	1 cut(s)	529
EcoRII	CCWGG	2 cut(s)	952, 1370
EcoT14I	CCWWGG	1 cut(s)	175
EcoT22I	ATGCAT	1 cut(s)	1363
EcoT38I	GRGCYC	2 cut(s)	1016, 1113
ErhI	CCWWGG	1 cut(s)	175
Esp3I	CGTCTC	1 cut(s)	1438
FaeI	CATG	4 cut(s)	212, 566, 1550, 1644
FalI	AAGNNNNNCTT	2 cut(s)	1088, 1120
FaqI	GGGAC	3 cut(s)	219, 788, 902
FatI	CATG	4 cut(s)	208, 562, 1546, 1640
FauI	CCCGC	1 cut(s)	1622
FokI	GGATG	8 cut(s)	399, 523, 737, 794, 821, 848, 914, 1370
FriOI	GRGCYC	2 cut(s)	1016, 1113
GsuI	CTGGAG	1 cut(s)	311
HaeIII	GGCC	2 cut(s)	373, 877
HapII	CCGG	4 cut(s)	322, 370, 440, 1493
HgaI	GACGC	1 cut(s)	8
Hin1I	GRCGYC	1 cut(s)	19
Hin1II	CATG	4 cut(s)	212, 566, 1550, 1644
HinfI	GANTC	8 cut(s)	24, 104, 131, 413, 673, 739, 1202, 1293
HpaII	CCGG	4 cut(s)	322, 370, 440, 1493
HphI	GGTGA	1 cut(s)	271
Hpy166II	GTNNAC	1 cut(s)	568
Hpy188I	TCNGA	4 cut(s)	91, 130, 550, 1288
Hpy188III	TCNNGA	8 cut(s)	328, 692, 743, 1051, 1061, 1087, 1138, 1511
Hpy8I	GTNNAC	1 cut(s)	568
Hpy99I	CGWCG	1 cut(s)	24
HpyCH4III	ACNGT	5 cut(s)	15, 192, 301, 312, 1507
HpyCH4IV	ACGT	1 cut(s)	1220
HpyCH4V	TGCA	7 cut(s)	254, 668, 785, 1147, 1277, 1361, 1535
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1532
HpyF3I	CTNAG	5 cut(s)	129, 864, 1092, 1242, 1263
HpySE526I	ACGT	1 cut(s)	1220
Hsp92I	GRCGYC	1 cut(s)	19
Hsp92II	CATG	4 cut(s)	212, 566, 1550, 1644
Kzo9I	GATC	5 cut(s)	75, 108, 703, 1134, 1374
LmnI	GCTCC	3 cut(s)	330, 1011, 1609
Lsp1109I	GCAGC	9 cut(s)	36, 47, 1105, 1261, 1513, 1519, 1572, 1585, 1588
LweI	GCATC	4 cut(s)	772, 799, 1056, 1348
MabI	ACCWGGT	1 cut(s)	952
MaeII	ACGT	1 cut(s)	1220
MaeIII	GTNAC	2 cut(s)	1037, 1501
MalI	GATC	5 cut(s)	77, 110, 705, 1136, 1376
MbiI	CCGCTC	1 cut(s)	730
MboI	GATC	5 cut(s)	75, 108, 703, 1134, 1374
MfeI	CAATTG	1 cut(s)	1419
MflI	RGATCY	2 cut(s)	75, 1134
MhlI	GDGCHC	4 cut(s)	519, 546, 1016, 1113
MluCI	AATT	8 cut(s)	401, 637, 687, 795, 1065, 1382, 1419, 1458
MlyI	GAGTC	3 cut(s)	140, 422, 1287
MmeI	TCCRAC	1 cut(s)	813
Mph1103I	ATGCAT	1 cut(s)	1363
MroXI	GAANNNNTTC	1 cut(s)	1316
MseI	TTAA	3 cut(s)	480, 759, 1467
MspA1I	CMGCKG	1 cut(s)	1563
MspI	CCGG	4 cut(s)	322, 370, 440, 1493
MspR9I	CCNGG	2 cut(s)	954, 1372
MunI	CAATTG	1 cut(s)	1419
MvaI	CCWGG	2 cut(s)	954, 1372
MwoI	GCNNNNNNNGC	1 cut(s)	1532
NdeII	GATC	5 cut(s)	75, 108, 703, 1134, 1374
NlaIII	CATG	4 cut(s)	212, 566, 1550, 1644
NlaIV	GGNNCC	6 cut(s)	326, 454, 531, 915, 951, 1013
NmuCI	GTSAC	1 cut(s)	1501
NsiI	ATGCAT	1 cut(s)	1363
NspV	TTCGAA	1 cut(s)	161
PaeR7I	CTCGAG	1 cut(s)	1059
PcsI	WCGNNNNNNNCGW	3 cut(s)	19, 1226, 1437
PdmI	GAANNNNTTC	1 cut(s)	1316
PfeI	GAWTC	5 cut(s)	24, 104, 673, 739, 1202
PleI	GAGTC	3 cut(s)	139, 421, 1287
PpsI	GAGTC	3 cut(s)	139, 421, 1287
PpuMI	RGGWCCY	1 cut(s)	529
PsiI	TTATAA	1 cut(s)	999
Psp5II	RGGWCCY	1 cut(s)	529
Psp6I	CCWGG	2 cut(s)	952, 1370
PspGI	CCWGG	2 cut(s)	952, 1370
PspN4I	GGNNCC	6 cut(s)	326, 454, 531, 915, 951, 1013
PspPI	GGNCC	3 cut(s)	319, 437, 529
PspPPI	RGGWCCY	1 cut(s)	529
PstI	CTGCAG	1 cut(s)	1279
PsuI	RGATCY	2 cut(s)	75, 1134
RsaI	GTAC	2 cut(s)	173, 365
RsaNI	GTAC	2 cut(s)	172, 364
SaqAI	TTAA	3 cut(s)	480, 759, 1467
Sau3AI	GATC	5 cut(s)	75, 108, 703, 1134, 1374
Sau96I	GGNCC	3 cut(s)	319, 437, 529
SchI	GAGTC	3 cut(s)	140, 422, 1287
ScrFI	CCNGG	2 cut(s)	954, 1372
SduI	GDGCHC	4 cut(s)	519, 546, 1016, 1113
SexAI	ACCWGGT	1 cut(s)	952
SfaNI	GCATC	4 cut(s)	772, 799, 1056, 1348
SfcI	CTRYAG	2 cut(s)	308, 1275
Sfr274I	CTCGAG	1 cut(s)	1059
SfuI	TTCGAA	1 cut(s)	161
SinI	GGWCC	3 cut(s)	319, 437, 529
SlaI	CTCGAG	1 cut(s)	1059
SmlI	CTYRAG	3 cut(s)	524, 1059, 1297
SmoI	CTYRAG	3 cut(s)	524, 1059, 1297
Sse9I	AATT	8 cut(s)	401, 637, 687, 795, 1065, 1382, 1419, 1458
SsiI	CCGC	8 cut(s)	348, 395, 730, 1496, 1529, 1563, 1615, 1621
SspI	AATATT	2 cut(s)	1158, 1414
StyD4I	CCNGG	2 cut(s)	952, 1370
StyI	CCWWGG	1 cut(s)	175
TaaI	ACNGT	5 cut(s)	15, 192, 301, 312, 1507
TaiI	ACGT	1 cut(s)	1223
TaqI	TCGA	8 cut(s)	22, 102, 107, 161, 647, 1060, 1086, 1320
TasI	AATT	8 cut(s)	401, 637, 687, 795, 1065, 1382, 1419, 1458
TauI	GCSGC	3 cut(s)	733, 1532, 1566
TfiI	GAWTC	5 cut(s)	24, 104, 673, 739, 1202
Tru1I	TTAA	3 cut(s)	480, 759, 1467
Tru9I	TTAA	3 cut(s)	480, 759, 1467
TscAI	CASTG	4 cut(s)	34, 261, 577, 1510
TseFI	GTSAC	1 cut(s)	1501
TseI	GCWGC	9 cut(s)	49, 60, 1118, 1274, 1526, 1532, 1560, 1598, 1601
Tsp45I	GTSAC	1 cut(s)	1501
TspDTI	ATGAA	6 cut(s)	458, 463, 909, 933, 995, 1566
TspGWI	ACGGA	4 cut(s)	529, 941, 1167, 1552
TspRI	CASTG	4 cut(s)	34, 261, 577, 1510
VpaK11BI	GGWCC	3 cut(s)	319, 437, 529
XapI	RAATTY	3 cut(s)	401, 637, 1065
XcmI	CCANNNNNNNNNTGG	1 cut(s)	943
XhoI	CTCGAG	1 cut(s)	1059
XmnI	GAANNNNTTC	1 cut(s)	1316
Zsp2I	ATGCAT	1 cut(s)	1363

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.