RchiOBHm_Chr7g0188351

Calnexin homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003158
Physical Location & Seq
Reverse (-)
7920037 .. 7923515
3479 bp
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UTR
Exon/CDS
Intron
PRQ16820

Sequence Viewer

Length: 1656 bp
ATGGCTGGGATTCGCTCCGGCGTCGTTTCGGTGTTGTTCGTGCTCGTTGCCTGCTTCTCTATTCAGCAGCTTTCTGCTTCCGACGATACGGTCTTCTACGATTCGTTCGACGAATCATTTGAAGGGCGTTGGACCGTGTCCGGCAAAGATGAATATCAAGGTGTTTGGAAGCACTCAAAGAGTGAGGGCCATGAGGATTTTGGGCTTCTTGTGAGTGAGAAGGCCAAGAAGTATGCAATAGTTAAGGAGCTTGATGAGCCTGTGAGCCTCAAGGATGGGACTGTTGTCCTTCAATTTGAGACTCGTCTCCAGAATGGGCTTGAATGTGGTGGTGCCTATCTGAAATACCTTCGACCCCAGGAGGCTGGATGGGAACCTAAGATATTTGACAATGAGTCTCCTTATTCTATCATGTTTGGACCTGACAAATGTGGGCTTACAAACAAGGTTCACTTCATTTTCAAGCATAAGAATCCCAAGAGTGGAGAGTATGTTGAACACCATCTTACCACCCCACCCTCTGTTCCAGCTGACAAACTTTCTCATGTATACACTGCCATTATCAAACCTGATAACGAGTTGATAATTCTGGTTGATGGAGAGGAGAAGAAGAAGGCCAACTTTTTATCAACTGATGATTTTATGCCTCCACTTATCCCTGCCAAGACAATTCCTGATCCAGAAGATAAGAAGCCTGAGGATTGGGATGAGAGAGCCAAAATTCCGGATCCTAATGCAGTTAAACCTGATGATTGGGATGAGGATGCACCCATGGAAATTGAAGACGATGAAGCAGAGAAACCTGAAGGATGGTTGGATGATGAGCCTGAGGAGGTCGATGATCCTGAAGCCACAAAGCCAGAGGATTGGGATGACGAGGAGGATGGTGAGTGGGAGGCCCCTAAGATTGACAACCCCAAGTGTGTGACTGCCCCTGGTTGTGGGGAATGGAAGAGGCCAATGAAGAGGAATCCAGCTTACAAGGGAAAATGGCATGCACCACTCATCGATAACCCCAGTTACAAGGGTATTTGGAAGCCTCAGGAGATTCCTAACCCCAGTTTCTTCGAGCTCGAGAAACCTAACTTTGAGCCCATTGCTGCTGTTGGTATTGAGATCTGGACCATGCAAGATGGTATATTGTTTGACAACATTTTGATAACAAAAGATGAGAAAGTTGCACAATCATACCGAGAGACCCAGTGGAAACCAAAATTTGAAGTTGAGAAGGAGAAAGAGAAGGCTGAGGAAGATGTTCCTAGTTCAGAAGGTCTATTAGCCAACGTCCAGAAGAAGATATTTGAAATTCTATACAAGGTTGCAGATGTTCCCTTCTTAGCTACCCACAAGTCTAAGATAATTGATCTCATTGAGAAGGCAGAGGAACAACCTAATATCACACTTGGTGTCATCGGCTCCATTGCTGTGATCATCTTAACAGCACTTTTCATGTTCCTTTTCGGTGGGAAGAAACCCACCAAGGTTGAGAAGAAACCCACCGTAGCCACTGAGGCTTCTGGAAGTGGTGTGGAGAAGAAAGAAGAGGAGAGTGAGAAGGAAGGTTCTTCAGGTGAGAAGGAAGGTTCTTCAGGTGAGAACGAGAAGGAAACTGGTGCTGCTCCTCGTCGGAGGACAGGACCCAGACGTGAAAACTGA

Protein Analysis

551

Amino Acids

62.03

Weight (kDa)

4.74

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calreticulin PF00262 32 - 387 1.7e-143 Calreticulin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 332
AccI GTMKAC 1 cut(s) 549
AccIII TCCGGA 1 cut(s) 724
AclWI GGATC 4 cut(s) 671, 722, 735, 836
AcsI RAATTY 3 cut(s) 720, 1214, 1305
AcuI CTGAAG 4 cut(s) 825, 867, 1551, 1572
AcyI GRCGYC 1 cut(s) 21
AdeI CACNNNGTG 1 cut(s) 1406
AfiI CCNNNNNNNGG 2 cut(s) 482, 941
AgsI TTSAA 8 cut(s) 122, 293, 323, 463, 497, 782, 1220, 1304
AhdI GACNNNNNGTC 1 cut(s) 394
AjiI CACGTC 1 cut(s) 1646
AjnI CCWGG 2 cut(s) 357, 934
AluBI AGCT 6 cut(s) 70, 250, 530, 977, 1072, 1340
AluI AGCT 6 cut(s) 70, 250, 530, 977, 1072, 1340
Alw21I GWGCWC 2 cut(s) 45, 1074
Alw26I GTCTC 4 cut(s) 293, 311, 402, 1190
AlwI GGATC 4 cut(s) 671, 722, 735, 836
Ama87I CYCGRG 1 cut(s) 1073
Aor13HI TCCGGA 1 cut(s) 724
AoxI GGCC 5 cut(s) 187, 222, 615, 897, 956
ApeKI GCWGC 3 cut(s) 67, 1100, 1616
ApoI RAATTY 3 cut(s) 720, 1214, 1305
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
Asp700I GAANNNNTTC 1 cut(s) 1254
AspS9I GGNCC 6 cut(s) 132, 187, 419, 898, 1122, 1637
AsuHPI GGTGA 3 cut(s) 899, 1583, 1604
AvaI CYCGRG 1 cut(s) 1073
AvaII GGWCC 4 cut(s) 132, 419, 1122, 1637
AxyI CCTNAGG 3 cut(s) 696, 828, 1041
BamHI GGATCC 1 cut(s) 727
BanI GGYRCC 1 cut(s) 332
BanII GRGCYC 2 cut(s) 1074, 1095
BbsI GAAGAC 2 cut(s) 85, 789
Bbv12I GWGCWC 2 cut(s) 45, 1074
BbvCI CCTCAGC 1 cut(s) 1245
BbvI GCAGC 3 cut(s) 79, 1087, 1603
BccI CCATC 7 cut(s) 269, 363, 510, 590, 804, 878, 1127
BciT130I CCWGG 2 cut(s) 359, 936
BclI TGATCA 1 cut(s) 1428
BcoDI GTCTC 4 cut(s) 293, 311, 402, 1190
BfaI CTAG 1 cut(s) 1260
BglI GCCNNNNNGGC 1 cut(s) 1511
BglII AGATCT 1 cut(s) 1116
BisI GCNGC 3 cut(s) 68, 1101, 1617
BlsI GCNGC 3 cut(s) 69, 1102, 1618
Bme1390I CCNGG 2 cut(s) 359, 936
Bme18I GGWCC 4 cut(s) 132, 419, 1122, 1637
BmeRI GACNNNNNGTC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 1073
BmgBI CACGTC 1 cut(s) 1646
BmgT120I GGNCC 6 cut(s) 132, 187, 419, 898, 1122, 1637
BmiI GGNNCC 6 cut(s) 334, 375, 729, 900, 1417, 1639
BmrFI CCNGG 2 cut(s) 359, 936
BmrI ACTGGG 3 cut(s) 1011, 1053, 1195
BmsI GCATC 1 cut(s) 754
BmuI ACTGGG 3 cut(s) 1011, 1053, 1195
BoxI GACNNNNGTC 1 cut(s) 284
BpiI GAAGAC 2 cut(s) 85, 789
BpmI CTGGAG 1 cut(s) 293
Bpu10I CCTNAGC 1 cut(s) 1245
BpuEI CTTGAG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 1008
BsaBI GATNNNNATC 1 cut(s) 153
BsaHI GRCGYC 1 cut(s) 21
BsaI GGTCTC 1 cut(s) 1190
BsaJI CCNNGG 4 cut(s) 357, 771, 934, 1479
BsaWI WCCGGW 1 cut(s) 724
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bsc4I CCNNNNNNNGG 2 cut(s) 482, 941
Bse1I ACTGG 4 cut(s) 1017, 1059, 1201, 1615
Bse21I CCTNAGG 3 cut(s) 696, 828, 1041
Bse3DI GCAATG 2 cut(s) 1095, 1419
Bse8I GATNNNNATC 1 cut(s) 153
BseAI TCCGGA 1 cut(s) 724
BseBI CCWGG 2 cut(s) 359, 936
BseCI ATCGAT 1 cut(s) 1008
BseDI CCNNGG 4 cut(s) 357, 771, 934, 1479
BseGI GGATG 9 cut(s) 280, 374, 712, 763, 769, 815, 823, 877, 889
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 2 cut(s) 482, 941
BseMI GCAATG 2 cut(s) 1095, 1419
BseMII CTCAG 5 cut(s) 687, 819, 1055, 1236, 1500
BseNI ACTGG 4 cut(s) 1017, 1059, 1201, 1615
BseRI GAGGAG 5 cut(s) 617, 845, 893, 1559, 1611
BseXI GCAGC 3 cut(s) 79, 1087, 1603
BseYI CCCAGC 1 cut(s) 5
BshFI GGCC 5 cut(s) 189, 224, 617, 899, 958
BshNI GGYRCC 1 cut(s) 332
BshVI ATCGAT 1 cut(s) 1008
BsiHKAI GWGCWC 2 cut(s) 45, 1074
BsiHKCI CYCGRG 1 cut(s) 1073
BsiSI CCGG 3 cut(s) 18, 141, 725
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 2 cut(s) 482, 941
BsmAI GTCTC 4 cut(s) 293, 311, 402, 1190
BsmBI CGTCTC 1 cut(s) 311
BsmFI GGGAC 1 cut(s) 292
BsnI GGCC 5 cut(s) 189, 224, 617, 899, 958
Bso31I GGTCTC 1 cut(s) 1190
BsoBI CYCGRG 1 cut(s) 1073
Bsp1286I GDGCHC 3 cut(s) 45, 1074, 1095
Bsp13I TCCGGA 1 cut(s) 724
Bsp143I GATC 6 cut(s) 676, 727, 841, 1116, 1363, 1428
Bsp19I CCATGG 1 cut(s) 771
BspANI GGCC 5 cut(s) 189, 224, 617, 899, 958
BspCNI CTCAG 5 cut(s) 688, 820, 1054, 1237, 1501
BspDI ATCGAT 1 cut(s) 1008
BspEI TCCGGA 1 cut(s) 724
BspLI GGNNCC 6 cut(s) 334, 375, 729, 900, 1417, 1639
BspPI GGATC 4 cut(s) 671, 722, 735, 836
BspT107I GGYRCC 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 1190
BsrDI GCAATG 2 cut(s) 1095, 1419
BsrI ACTGG 4 cut(s) 1017, 1059, 1201, 1615
BssECI CCNNGG 4 cut(s) 357, 771, 934, 1479
BssMI GATC 6 cut(s) 676, 727, 841, 1116, 1363, 1428
BssNAI GTATAC 1 cut(s) 550
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 2 cut(s) 771, 1479
Bst1107I GTATAC 1 cut(s) 550
Bst2UI CCWGG 2 cut(s) 359, 936
Bst4CI ACNGT 4 cut(s) 91, 136, 283, 1501
Bst6I CTCTTC 3 cut(s) 947, 959, 1536
BstACI GRCGYC 1 cut(s) 21
BstC8I GCNNGC 2 cut(s) 52, 996
BstDEI CTNAG 9 cut(s) 378, 696, 828, 903, 1041, 1245, 1336, 1353, 1509
BstDSI CCRYGG 1 cut(s) 771
BstF5I GGATG 9 cut(s) 280, 374, 712, 763, 769, 815, 823, 877, 889
BstKTI GATC 6 cut(s) 679, 730, 844, 1119, 1366, 1431
BstMAI GTCTC 4 cut(s) 293, 311, 402, 1190
BstMBI GATC 6 cut(s) 676, 727, 841, 1116, 1363, 1428
BstMWI GCNNNNNNNGC 2 cut(s) 256, 1511
BstNI CCWGG 2 cut(s) 359, 936
BstNSI RCATGY 1 cut(s) 998
BstPAI GACNNNNGTC 1 cut(s) 284
BstSCI CCNGG 2 cut(s) 357, 934
BstV1I GCAGC 3 cut(s) 79, 1087, 1603
BstV2I GAAGAC 2 cut(s) 85, 789
BstX2I RGATCY 2 cut(s) 727, 1116
BstXI CCANNNNNNTGG 2 cut(s) 365, 867
BstYI RGATCY 2 cut(s) 727, 1116
BstZ17I GTATAC 1 cut(s) 550
Bsu15I ATCGAT 1 cut(s) 1008
Bsu36I CCTNAGG 3 cut(s) 696, 828, 1041
BsuRI GGCC 5 cut(s) 189, 224, 617, 899, 958
BsuTUI ATCGAT 1 cut(s) 1008
BtgI CCRYGG 1 cut(s) 771
BtrI CACGTC 1 cut(s) 1646
BtsCI GGATG 9 cut(s) 280, 374, 712, 763, 769, 815, 823, 877, 889
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 3 cut(s) 552, 1208, 1506
Cac8I GCNNGC 2 cut(s) 52, 996
Cfr13I GGNCC 6 cut(s) 132, 187, 419, 898, 1122, 1637
ClaI ATCGAT 1 cut(s) 1008
CseI GACGC 1 cut(s) 10
CspCI CAANNNNNGTGG 2 cut(s) 1465, 1500
CviAII CATG 7 cut(s) 191, 412, 545, 772, 995, 1126, 1450
DdeI CTNAG 9 cut(s) 378, 696, 828, 903, 1041, 1245, 1336, 1353, 1509
DpnI GATC 6 cut(s) 678, 729, 843, 1118, 1365, 1430
DpnII GATC 6 cut(s) 676, 727, 841, 1116, 1363, 1428
DraIII CACNNNGTG 1 cut(s) 1406
DrdI GACNNNNNNGTC 1 cut(s) 89
DriI GACNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 89
Eam1104I CTCTTC 3 cut(s) 947, 959, 1536
Eam1105I GACNNNNNGTC 1 cut(s) 394
EarI CTCTTC 3 cut(s) 947, 959, 1536
Ecl136II GAGCTC 1 cut(s) 1072
Eco130I CCWWGG 2 cut(s) 771, 1479
Eco24I GRGCYC 2 cut(s) 1074, 1095
Eco31I GGTCTC 1 cut(s) 1190
Eco47I GGWCC 4 cut(s) 132, 419, 1122, 1637
Eco53kI GAGCTC 1 cut(s) 1072
Eco57I CTGAAG 4 cut(s) 825, 867, 1551, 1572
Eco81I CCTNAGG 3 cut(s) 696, 828, 1041
Eco88I CYCGRG 1 cut(s) 1073
EcoICRI GAGCTC 1 cut(s) 1072
EcoO109I RGGNCCY 2 cut(s) 898, 1637
EcoRII CCWGG 2 cut(s) 357, 934
EcoT14I CCWWGG 2 cut(s) 771, 1479
EcoT38I GRGCYC 2 cut(s) 1074, 1095
ErhI CCWWGG 2 cut(s) 771, 1479
Esp3I CGTCTC 1 cut(s) 311
FaeI CATG 7 cut(s) 194, 415, 548, 775, 998, 1129, 1453
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 605, 637
FaqI GGGAC 1 cut(s) 292
FatI CATG 7 cut(s) 190, 411, 544, 771, 994, 1125, 1449
FbaI TGATCA 1 cut(s) 1428
FblI GTMKAC 1 cut(s) 549
Fnu4HI GCNGC 3 cut(s) 68, 1101, 1617
FokI GGATG 9 cut(s) 287, 381, 719, 770, 776, 822, 830, 884, 896
FriOI GRGCYC 2 cut(s) 1074, 1095
Fsp4HI GCNGC 3 cut(s) 68, 1101, 1617
FspBI CTAG 1 cut(s) 1260
GluI GCNGC 3 cut(s) 68, 1101, 1617
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 293
HaeIII GGCC 5 cut(s) 189, 224, 617, 899, 958
HapII CCGG 3 cut(s) 18, 141, 725
HgaI GACGC 1 cut(s) 10
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 7 cut(s) 194, 415, 548, 775, 998, 1129, 1453
HinfI GANTC 8 cut(s) 10, 101, 113, 301, 395, 472, 970, 1048
HpaII CCGG 3 cut(s) 18, 141, 725
HphI GGTGA 3 cut(s) 899, 1583, 1604
Hpy166II GTNNAC 2 cut(s) 451, 550
Hpy188I TCNGA 4 cut(s) 82, 342, 1267, 1629
Hpy8I GTNNAC 2 cut(s) 451, 550
Hpy99I CGWCG 4 cut(s) 26, 86, 113, 1629
HpyCH4III ACNGT 4 cut(s) 91, 136, 283, 1501
HpyCH4IV ACGT 2 cut(s) 1284, 1645
HpyCH4V TGCA 7 cut(s) 236, 737, 767, 998, 1129, 1181, 1322
HpyF10VI GCNNNNNNNGC 2 cut(s) 256, 1511
HpyF3I CTNAG 9 cut(s) 378, 696, 828, 903, 1041, 1245, 1336, 1353, 1509
HpySE526I ACGT 2 cut(s) 1284, 1645
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 7 cut(s) 194, 415, 548, 775, 998, 1129, 1453
Kpn2I TCCGGA 1 cut(s) 724
Ksp22I TGATCA 1 cut(s) 1428
Kzo9I GATC 6 cut(s) 676, 727, 841, 1116, 1363, 1428
LmnI GCTCC 4 cut(s) 20, 247, 1421, 1624
Lsp1109I GCAGC 3 cut(s) 79, 1087, 1603
LweI GCATC 1 cut(s) 754
MaeI CTAG 1 cut(s) 1260
MaeII ACGT 2 cut(s) 1284, 1645
MaeIII GTNAC 2 cut(s) 925, 1019
MalI GATC 6 cut(s) 678, 729, 843, 1118, 1365, 1430
MboI GATC 6 cut(s) 676, 727, 841, 1116, 1363, 1428
MflI RGATCY 2 cut(s) 727, 1116
MhlI GDGCHC 3 cut(s) 45, 1074, 1095
MluCI AATT 8 cut(s) 293, 585, 669, 720, 777, 1214, 1305, 1359
MlyI GAGTC 2 cut(s) 295, 404
MmeI TCCRAC 4 cut(s) 105, 110, 795, 1607
MroI TCCGGA 1 cut(s) 724
MroXI GAANNNNTTC 1 cut(s) 1254
MseI TTAA 3 cut(s) 243, 741, 1436
MslI CAYNNNNRTG 1 cut(s) 1424
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 3 cut(s) 18, 141, 725
MspR9I CCNGG 2 cut(s) 359, 936
MvaI CCWGG 2 cut(s) 359, 936
MwoI GCNNNNNNNGC 2 cut(s) 256, 1511
NcoI CCATGG 1 cut(s) 771
NdeII GATC 6 cut(s) 676, 727, 841, 1116, 1363, 1428
NlaIII CATG 7 cut(s) 194, 415, 548, 775, 998, 1129, 1453
NlaIV GGNNCC 6 cut(s) 334, 375, 729, 900, 1417, 1639
NmuCI GTSAC 1 cut(s) 925
NspI RCATGY 1 cut(s) 998
PaeI GCATGC 1 cut(s) 998
PaeR7I CTCGAG 1 cut(s) 1073
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 1254
PfeI GAWTC 6 cut(s) 10, 101, 113, 472, 970, 1048
PflFI GACNNNGTC 1 cut(s) 136
PkrI GCNGC 3 cut(s) 69, 1102, 1618
PleI GAGTC 2 cut(s) 295, 403
PpsI GAGTC 2 cut(s) 295, 403
PpuMI RGGWCCY 1 cut(s) 1637
PshAI GACNNNNGTC 1 cut(s) 284
Psp124BI GAGCTC 1 cut(s) 1074
Psp5II RGGWCCY 1 cut(s) 1637
Psp6I CCWGG 2 cut(s) 357, 934
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 2 cut(s) 357, 934
PspN4I GGNNCC 6 cut(s) 334, 375, 729, 900, 1417, 1639
PspPI GGNCC 6 cut(s) 132, 187, 419, 898, 1122, 1637
PspPPI RGGWCCY 1 cut(s) 1637
PsuI RGATCY 2 cut(s) 727, 1116
PsyI GACNNNGTC 1 cut(s) 136
PvuII CAGCTG 1 cut(s) 530
RseI CAYNNNNRTG 1 cut(s) 1424
SacI GAGCTC 1 cut(s) 1074
SaqAI TTAA 3 cut(s) 243, 741, 1436
SatI GCNGC 3 cut(s) 68, 1101, 1617
Sau3AI GATC 6 cut(s) 676, 727, 841, 1116, 1363, 1428
Sau96I GGNCC 6 cut(s) 132, 187, 419, 898, 1122, 1637
SchI GAGTC 2 cut(s) 295, 404
ScrFI CCNGG 2 cut(s) 359, 936
SduI GDGCHC 3 cut(s) 45, 1074, 1095
SfaNI GCATC 1 cut(s) 754
Sfr274I CTCGAG 1 cut(s) 1073
SinI GGWCC 4 cut(s) 132, 419, 1122, 1637
SlaI CTCGAG 1 cut(s) 1073
SmiMI CAYNNNNRTG 1 cut(s) 1424
SmlI CTYRAG 2 cut(s) 269, 1073
SmoI CTYRAG 2 cut(s) 269, 1073
SphI GCATGC 1 cut(s) 998
Sse9I AATT 8 cut(s) 293, 585, 669, 720, 777, 1214, 1305, 1359
SspMI CTAG 1 cut(s) 1260
SstI GAGCTC 1 cut(s) 1074
StyD4I CCNGG 2 cut(s) 357, 934
StyI CCWWGG 2 cut(s) 771, 1479
TaaI ACNGT 4 cut(s) 91, 136, 283, 1501
TaiI ACGT 2 cut(s) 1287, 1648
TaqI TCGA 6 cut(s) 108, 352, 837, 1008, 1068, 1074
TasI AATT 8 cut(s) 293, 585, 669, 720, 777, 1214, 1305, 1359
TfiI GAWTC 6 cut(s) 10, 101, 113, 472, 970, 1048
Tru1I TTAA 3 cut(s) 243, 741, 1436
Tru9I TTAA 3 cut(s) 243, 741, 1436
TscAI CASTG 3 cut(s) 559, 1208, 1513
TseFI GTSAC 1 cut(s) 925
TseI GCWGC 3 cut(s) 67, 1100, 1616
Tsp45I GTSAC 1 cut(s) 925
TspDTI ATGAA 5 cut(s) 165, 445, 804, 977, 1438
TspRI CASTG 3 cut(s) 559, 1208, 1513
Tth111I GACNNNGTC 1 cut(s) 136
VpaK11BI GGWCC 4 cut(s) 132, 419, 1122, 1637
XapI RAATTY 3 cut(s) 720, 1214, 1305
XceI RCATGY 1 cut(s) 998
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XhoI CTCGAG 1 cut(s) 1073
XmiI GTMKAC 1 cut(s) 549
XmnI GAANNNNTTC 1 cut(s) 1254
XspI CTAG 1 cut(s) 1260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.