Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003158

Physical Location & Seq

Genomic Coordinates Forward (+)

61584700 .. 61587485

Gene Structure 2786 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004706

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1665 bp

ATGGCTGGGATTCGCTCCGGCGTCGTTTCGGTGCTCTTCGCGCTCTTCGCGCTCTTCGCTTGCTTCTCTATTCAGCAGCTTTCTGCTTCTGACGATACGGTCTTTTACGATTCGTTCGACGAATCATTTGAAGGGCGTTGGACCGTGTCCGGCAAAGATGACTATCAAGGTGTTTGGAAGCACTCAAAGAGTGAGGGCCATGAGGATTTTGGGCTTCTTGTGAGTGAGAAGGCCAAGAAGTATGCAATAGTTAAGGAGCTTGATGAGCCTGTGAGCCTCAAGGATGGGACTGTTGTCCTTCAATTTGAGACTCGTCTCCAGAATGGGCTTGAATGTGGTGGTGCCTATCTGAAATACCTTCGACCCCAGGAGGCTGGATGGGAACCTAAGATATTTGACAATGAGTCTCCTTATTCTATCATGTTTGGACCTGACAAATGTGGGCTTACAAACAAGGTTCACTTCATTTTCAAGCATAAGAATCCCAAGAGTGGAGAGTATGTTGAACACCATCTTACCACCCCACCCTCTGTTCCAACTGACAAACTTTCTCATGTATATACTGCCATTATCAAACCTGATAACGAGTTGATAATTCTGGTTGATGGAGAGGAGAAGAAGAAGGCCAACTTTTTATCAACTGATGATTTTATGCCTCCACTTATCCCTGCCAAGACAATTCCTGATCCAGAAGATAAGAAGCCTGAGGATTGGGATGAGAGAGCCAAAATTCCGGATCCTAATGCAGTTAAACCTGATGATTGGGATGAGGATGCACCCATGGAAATTGAAGATGAGGAAGCAGAGAAACCTGAAGGATGGTTGGATGATGAGCCTGAGGAGGTAGATGATCCTGAAGCCACAAAGCCAGAGGATTGGGATGATGAGGAGGATGGTGAGTGGGAGGCCCCTAAGATTGACAACCCCAAGTGTGTGACTGCCCCTGGTTGTGGTGAATGGAAGAGGCCAATGAAGAGGAATCCAGCTTACAAGGGAAAATGGCATGCACCACTCATCGATAACCCCAGTTACAAGGGTGTTTGGAAGCCTCAGGAGATTCCTAACCCCAGTTACTTCGAGCTTGAGAAACCTGACTTCGAACCCATTGCTGCTGTTGGTATTGAGATCTGGACCATGCAAGATGGTATATTGTTTGACAACATTTTGATAACAAAAGATGAGAAAGTTGCACAATCATACCGAGAGACCCAGTGGAAACCAAAATTTGAAGTTGAGAAGGAGAAAGAGAAGGCTGAGGAAGATGTTCCTAGTTCAGAAGGTCTATTAGCCAAGGTCCAGAAGAAGATATTTGAAATTCTATACAAGGTTGCAGATGTTCCCTTCTTAGCTGCCCACAAGTCTAAGATAATTGATCTCATTGAGAAGGCAGAGGAACAACCTAATATCACACTTGGTGTCATTGGCTCCATTGCTGTGATCATCTTAACAGCACTTTTCATGTTCCTTTTCGGTGGGAAGAAACCCACAAAGGTTGAGAAGAAACCCGCCGTAGCCACTGAGGCTTCTGGGAGTGGTGTGGAGAAGAAAGAAGAGGAGAGTGAGAAGGAAGGTTCTTCAGGTGAGAAGGAAGGTTCTTCAGGTGAGAACGAGAAGGAAACTGGTGCTGCTCCTCGTCGGAGGACAGGACCCAGACGTGAAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005783 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0009507 GO:0009536 GO:0012505 GO:0016020 GO:0030054 GO:0030312 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04141 ko04145 ko04612 ko04918 ko05166 map04141 map04145 map04612 map04918 map05166

Pfam Domains

Protein Families

Calreticulin

Protein Analysis

555

Amino Acids

62.37

Weight (kDa)

4.74

Isoelectric Point (pI)

44.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Calreticulin	PF00262	35 - 390	3.4e-144	Calreticulin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003158)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07340 AT5G07340 AT5G61790
fragaria_vesca	FvH4_4g22170 FvH4_5g09940
malus_domestica	MD00G1076300.v1.1 MD08G1097600.v1.1 MD08G1097700.v1.1 MD13G1134200.v1.1 MD14G1144700.v1.1 MD16G1144800.v1.1
prunus_persica	Prupe.1G211400_v2.0.a1 Prupe.5G139100_v2.0.a1 Prupe.5G139100_v2.0.a1
pyrus_communis	pycom06g11940 pycom13g11700 pycom14g12020 pycom16g11430
rosa_chinensis	RchiOBHm_Chr4g0428401 RchiOBHm_Chr7g0188351
rosa_laevigata	RLG00000004706 RLG00000007154
rosa_multiflora	Rmu_sc0004698.1_g000004 Rmu_sc0008750.1_g000004
rosa_roxburghii	Rroxscaffold_3G00266480 Rroxscaffold_5G00370070
rosa_rugosa	Rorug04G0225200 Rorug06G0490000 Rorug06G0490100
rosa_samantha	Rh4AG282400 Rh4BG288000 Rh4CG303600 Rh4DG285100 Rh7BG096200 Rh7CG096500
rosa_wichuraiana	Rw4G024510 Rw7G008160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	98
AccB1I	GGYRCC	1 cut(s)	341
AccII	CGCG	2 cut(s)	41, 50
AccIII	TCCGGA	1 cut(s)	733
AciI	CCGC	1 cut(s)	1506
AclWI	GGATC	4 cut(s)	680, 731, 744, 845
AcsI	RAATTY	3 cut(s)	729, 1223, 1314
AcuI	CTGAAG	4 cut(s)	834, 876, 1560, 1581
AcyI	GRCGYC	1 cut(s)	21
AdeI	CACNNNGTG	1 cut(s)	1415
AfiI	CCNNNNNNNGG	2 cut(s)	491, 950
AgsI	TTSAA	8 cut(s)	131, 302, 332, 472, 506, 791, 1229, 1313
AhdI	GACNNNNNGTC	1 cut(s)	403
AjiI	CACGTC	1 cut(s)	1655
AjnI	CCWGG	2 cut(s)	366, 943
AluBI	AGCT	5 cut(s)	79, 259, 986, 1081, 1349
AluI	AGCT	5 cut(s)	79, 259, 986, 1081, 1349
Alw21I	GWGCWC	1 cut(s)	36
Alw26I	GTCTC	4 cut(s)	302, 320, 411, 1199
AlwI	GGATC	4 cut(s)	680, 731, 744, 845
Aor13HI	TCCGGA	1 cut(s)	733
AoxI	GGCC	5 cut(s)	196, 231, 624, 906, 965
ApeKI	GCWGC	4 cut(s)	76, 1109, 1349, 1625
ApoI	RAATTY	3 cut(s)	729, 1223, 1314
ArsI	GACNNNNNNTTYG	2 cut(s)	110, 142
Asp700I	GAANNNNTTC	1 cut(s)	1263
AspLEI	GCGC	2 cut(s)	43, 52
AspS9I	GGNCC	7 cut(s)	141, 196, 428, 907, 1131, 1294, 1646
AsuHPI	GGTGA	4 cut(s)	908, 965, 1592, 1613
AsuII	TTCGAA	1 cut(s)	1098
AvaII	GGWCC	5 cut(s)	141, 428, 1131, 1294, 1646
AxyI	CCTNAGG	3 cut(s)	705, 837, 1050
BamHI	GGATCC	1 cut(s)	736
BanI	GGYRCC	1 cut(s)	341
Bbv12I	GWGCWC	1 cut(s)	36
BbvCI	CCTCAGC	1 cut(s)	1254
BbvI	GCAGC	4 cut(s)	88, 1096, 1336, 1612
BccI	CCATC	7 cut(s)	278, 372, 519, 599, 813, 887, 1136
BceAI	ACGGC	1 cut(s)	1493
BcgI	CGANNNNNNTGC	2 cut(s)	1088, 1122
BciT130I	CCWGG	2 cut(s)	368, 945
BclI	TGATCA	1 cut(s)	1437
BcoDI	GTCTC	4 cut(s)	302, 320, 411, 1199
BfaI	CTAG	1 cut(s)	1269
BglI	GCCNNNNNGGC	1 cut(s)	1520
BglII	AGATCT	1 cut(s)	1125
BisI	GCNGC	4 cut(s)	77, 1110, 1350, 1626
BlsI	GCNGC	4 cut(s)	78, 1111, 1351, 1627
Bme1390I	CCNGG	2 cut(s)	368, 945
Bme18I	GGWCC	5 cut(s)	141, 428, 1131, 1294, 1646
BmeRI	GACNNNNNGTC	1 cut(s)	403
BmgBI	CACGTC	1 cut(s)	1655
BmgT120I	GGNCC	7 cut(s)	141, 196, 428, 907, 1131, 1294, 1646
BmiI	GGNNCC	6 cut(s)	343, 384, 738, 909, 1426, 1648
BmrFI	CCNGG	2 cut(s)	368, 945
BmrI	ACTGGG	3 cut(s)	1020, 1062, 1204
BmsI	GCATC	1 cut(s)	763
BmuI	ACTGGG	3 cut(s)	1020, 1062, 1204
BoxI	GACNNNNGTC	1 cut(s)	293
BpmI	CTGGAG	1 cut(s)	302
Bpu10I	CCTNAGC	1 cut(s)	1254
Bpu14I	TTCGAA	1 cut(s)	1098
BpuEI	CTTGAG	2 cut(s)	263, 1103
Bsa29I	ATCGAT	1 cut(s)	1017
BsaBI	GATNNNNATC	1 cut(s)	162
BsaHI	GRCGYC	1 cut(s)	21
BsaI	GGTCTC	1 cut(s)	1199
BsaJI	CCNNGG	4 cut(s)	366, 780, 943, 1290
BsaWI	WCCGGW	1 cut(s)	733
BsaXI	ACNNNNNCTCC	2 cut(s)	486, 516
Bsc4I	CCNNNNNNNGG	2 cut(s)	491, 950
Bse1I	ACTGG	4 cut(s)	1026, 1068, 1210, 1624
Bse21I	CCTNAGG	3 cut(s)	705, 837, 1050
Bse3DI	GCAATG	2 cut(s)	1104, 1428
Bse8I	GATNNNNATC	1 cut(s)	162
BseAI	TCCGGA	1 cut(s)	733
BseBI	CCWGG	2 cut(s)	368, 945
BseCI	ATCGAT	1 cut(s)	1017
BseDI	CCNNGG	4 cut(s)	366, 780, 943, 1290
BseGI	GGATG	9 cut(s)	289, 383, 721, 772, 778, 824, 832, 886, 898
BseJI	GATNNNNATC	1 cut(s)	162
BseLI	CCNNNNNNNGG	2 cut(s)	491, 950
BseMI	GCAATG	2 cut(s)	1104, 1428
BseMII	CTCAG	5 cut(s)	696, 828, 1064, 1245, 1509
BseNI	ACTGG	4 cut(s)	1026, 1068, 1210, 1624
BseRI	GAGGAG	5 cut(s)	626, 854, 902, 1568, 1620
BseXI	GCAGC	4 cut(s)	88, 1096, 1336, 1612
BseYI	CCCAGC	1 cut(s)	5
Bsh1236I	CGCG	2 cut(s)	41, 50
BshFI	GGCC	5 cut(s)	198, 233, 626, 908, 967
BshNI	GGYRCC	1 cut(s)	341
BshVI	ATCGAT	1 cut(s)	1017
BsiHKAI	GWGCWC	1 cut(s)	36
BsiSI	CCGG	3 cut(s)	18, 150, 734
BslFI	GGGAC	1 cut(s)	301
BslI	CCNNNNNNNGG	2 cut(s)	491, 950
BsmAI	GTCTC	4 cut(s)	302, 320, 411, 1199
BsmBI	CGTCTC	1 cut(s)	320
BsmFI	GGGAC	1 cut(s)	301
BsnI	GGCC	5 cut(s)	198, 233, 626, 908, 967
Bso31I	GGTCTC	1 cut(s)	1199
Bsp119I	TTCGAA	1 cut(s)	1098
Bsp1286I	GDGCHC	1 cut(s)	36
Bsp13I	TCCGGA	1 cut(s)	733
Bsp143I	GATC	6 cut(s)	685, 736, 850, 1125, 1372, 1437
Bsp19I	CCATGG	1 cut(s)	780
BspACI	CCGC	1 cut(s)	1506
BspANI	GGCC	5 cut(s)	198, 233, 626, 908, 967
BspCNI	CTCAG	5 cut(s)	697, 829, 1063, 1246, 1510
BspDI	ATCGAT	1 cut(s)	1017
BspEI	TCCGGA	1 cut(s)	733
BspFNI	CGCG	2 cut(s)	41, 50
BspLI	GGNNCC	6 cut(s)	343, 384, 738, 909, 1426, 1648
BspPI	GGATC	4 cut(s)	680, 731, 744, 845
BspQI	GCTCTTC	3 cut(s)	41, 50, 59
BspT104I	TTCGAA	1 cut(s)	1098
BspT107I	GGYRCC	1 cut(s)	341
BspTNI	GGTCTC	1 cut(s)	1199
BsrDI	GCAATG	2 cut(s)	1104, 1428
BsrI	ACTGG	4 cut(s)	1026, 1068, 1210, 1624
BssECI	CCNNGG	4 cut(s)	366, 780, 943, 1290
BssMI	GATC	6 cut(s)	685, 736, 850, 1125, 1372, 1437
BssNI	GRCGYC	1 cut(s)	21
BssT1I	CCWWGG	2 cut(s)	780, 1290
Bst2UI	CCWGG	2 cut(s)	368, 945
Bst4CI	ACNGT	3 cut(s)	100, 145, 292
Bst6I	CTCTTC	6 cut(s)	41, 50, 59, 956, 968, 1545
BstACI	GRCGYC	1 cut(s)	21
BstBI	TTCGAA	1 cut(s)	1098
BstC8I	GCNNGC	2 cut(s)	61, 1005
BstDEI	CTNAG	9 cut(s)	387, 705, 837, 912, 1050, 1254, 1345, 1362, 1518
BstDSI	CCRYGG	1 cut(s)	780
BstF5I	GGATG	9 cut(s)	289, 383, 721, 772, 778, 824, 832, 886, 898
BstFNI	CGCG	2 cut(s)	41, 50
BstHHI	GCGC	2 cut(s)	43, 52
BstKTI	GATC	6 cut(s)	688, 739, 853, 1128, 1375, 1440
BstMAI	GTCTC	4 cut(s)	302, 320, 411, 1199
BstMBI	GATC	6 cut(s)	685, 736, 850, 1125, 1372, 1437
BstMWI	GCNNNNNNNGC	6 cut(s)	40, 47, 49, 56, 265, 1520
BstNI	CCWGG	2 cut(s)	368, 945
BstNSI	RCATGY	1 cut(s)	1007
BstPAI	GACNNNNGTC	1 cut(s)	293
BstSCI	CCNGG	2 cut(s)	366, 943
BstUI	CGCG	2 cut(s)	41, 50
BstV1I	GCAGC	4 cut(s)	88, 1096, 1336, 1612
BstX2I	RGATCY	2 cut(s)	736, 1125
BstXI	CCANNNNNNTGG	2 cut(s)	374, 876
BstYI	RGATCY	2 cut(s)	736, 1125
Bsu15I	ATCGAT	1 cut(s)	1017
Bsu36I	CCTNAGG	3 cut(s)	705, 837, 1050
BsuRI	GGCC	5 cut(s)	198, 233, 626, 908, 967
BsuTUI	ATCGAT	1 cut(s)	1017
BtgI	CCRYGG	1 cut(s)	780
BtrI	CACGTC	1 cut(s)	1655
BtsCI	GGATG	9 cut(s)	289, 383, 721, 772, 778, 824, 832, 886, 898
BtsIMutI	CAGTG	2 cut(s)	1217, 1515
Cac8I	GCNNGC	2 cut(s)	61, 1005
CfoI	GCGC	2 cut(s)	43, 52
Cfr13I	GGNCC	7 cut(s)	141, 196, 428, 907, 1131, 1294, 1646
ClaI	ATCGAT	1 cut(s)	1017
CseI	GACGC	1 cut(s)	10
CspCI	CAANNNNNGTGG	2 cut(s)	1474, 1509
CviAII	CATG	7 cut(s)	200, 421, 554, 781, 1004, 1135, 1459
DdeI	CTNAG	9 cut(s)	387, 705, 837, 912, 1050, 1254, 1345, 1362, 1518
DpnI	GATC	6 cut(s)	687, 738, 852, 1127, 1374, 1439
DpnII	GATC	6 cut(s)	685, 736, 850, 1125, 1372, 1437
DraIII	CACNNNGTG	1 cut(s)	1415
DrdI	GACNNNNNNGTC	1 cut(s)	98
DriI	GACNNNNNGTC	1 cut(s)	403
DseDI	GACNNNNNNGTC	1 cut(s)	98
Eam1104I	CTCTTC	6 cut(s)	41, 50, 59, 956, 968, 1545
Eam1105I	GACNNNNNGTC	1 cut(s)	403
EarI	CTCTTC	6 cut(s)	41, 50, 59, 956, 968, 1545
Eco130I	CCWWGG	2 cut(s)	780, 1290
Eco31I	GGTCTC	1 cut(s)	1199
Eco47I	GGWCC	5 cut(s)	141, 428, 1131, 1294, 1646
Eco57I	CTGAAG	4 cut(s)	834, 876, 1560, 1581
Eco81I	CCTNAGG	3 cut(s)	705, 837, 1050
EcoO109I	RGGNCCY	2 cut(s)	907, 1646
EcoRII	CCWGG	2 cut(s)	366, 943
EcoT14I	CCWWGG	2 cut(s)	780, 1290
ErhI	CCWWGG	2 cut(s)	780, 1290
Esp3I	CGTCTC	1 cut(s)	320
FaeI	CATG	7 cut(s)	203, 424, 557, 784, 1007, 1138, 1462
FalI	AAGNNNNNCTT	4 cut(s)	446, 478, 614, 646
FaqI	GGGAC	1 cut(s)	301
FatI	CATG	7 cut(s)	199, 420, 553, 780, 1003, 1134, 1458
FauI	CCCGC	1 cut(s)	1513
FbaI	TGATCA	1 cut(s)	1437
Fnu4HI	GCNGC	4 cut(s)	77, 1110, 1350, 1626
FokI	GGATG	9 cut(s)	296, 390, 728, 779, 785, 831, 839, 893, 905
Fsp4HI	GCNGC	4 cut(s)	77, 1110, 1350, 1626
FspBI	CTAG	1 cut(s)	1269
GlaI	GCGC	2 cut(s)	42, 51
GluI	GCNGC	4 cut(s)	77, 1110, 1350, 1626
GsaI	CCCAGC	1 cut(s)	9
GsuI	CTGGAG	1 cut(s)	302
HaeIII	GGCC	5 cut(s)	198, 233, 626, 908, 967
HapII	CCGG	3 cut(s)	18, 150, 734
HgaI	GACGC	1 cut(s)	10
HhaI	GCGC	2 cut(s)	43, 52
Hin1I	GRCGYC	1 cut(s)	21
Hin1II	CATG	7 cut(s)	203, 424, 557, 784, 1007, 1138, 1462
Hin6I	GCGC	2 cut(s)	41, 50
HinP1I	GCGC	2 cut(s)	41, 50
HinfI	GANTC	8 cut(s)	10, 110, 122, 310, 404, 481, 979, 1057
HpaII	CCGG	3 cut(s)	18, 150, 734
HphI	GGTGA	4 cut(s)	908, 965, 1592, 1613
Hpy166II	GTNNAC	1 cut(s)	460
Hpy188I	TCNGA	4 cut(s)	91, 351, 1276, 1638
Hpy188III	TCNNGA	8 cut(s)	319, 683, 689, 734, 854, 1052, 1129, 1297
Hpy8I	GTNNAC	1 cut(s)	460
Hpy99I	CGWCG	3 cut(s)	26, 122, 1638
HpyCH4III	ACNGT	3 cut(s)	100, 145, 292
HpyCH4IV	ACGT	1 cut(s)	1654
HpyCH4V	TGCA	7 cut(s)	245, 746, 776, 1007, 1138, 1190, 1331
HpyF10VI	GCNNNNNNNGC	6 cut(s)	40, 47, 49, 56, 265, 1520
HpyF3I	CTNAG	9 cut(s)	387, 705, 837, 912, 1050, 1254, 1345, 1362, 1518
HpySE526I	ACGT	1 cut(s)	1654
Hsp92I	GRCGYC	1 cut(s)	21
Hsp92II	CATG	7 cut(s)	203, 424, 557, 784, 1007, 1138, 1462
HspAI	GCGC	2 cut(s)	41, 50
Kpn2I	TCCGGA	1 cut(s)	733
Ksp22I	TGATCA	1 cut(s)	1437
Kzo9I	GATC	6 cut(s)	685, 736, 850, 1125, 1372, 1437
LguI	GCTCTTC	3 cut(s)	41, 50, 59
LmnI	GCTCC	4 cut(s)	20, 256, 1430, 1633
Lsp1109I	GCAGC	4 cut(s)	88, 1096, 1336, 1612
LweI	GCATC	1 cut(s)	763
MaeI	CTAG	1 cut(s)	1269
MaeII	ACGT	1 cut(s)	1654
MaeIII	GTNAC	3 cut(s)	934, 1028, 1070
MalI	GATC	6 cut(s)	687, 738, 852, 1127, 1374, 1439
MboI	GATC	6 cut(s)	685, 736, 850, 1125, 1372, 1437
MflI	RGATCY	2 cut(s)	736, 1125
MhlI	GDGCHC	1 cut(s)	36
MluCI	AATT	8 cut(s)	302, 594, 678, 729, 786, 1223, 1314, 1368
MlyI	GAGTC	2 cut(s)	304, 413
MmeI	TCCRAC	4 cut(s)	119, 560, 804, 1616
MroI	TCCGGA	1 cut(s)	733
MroXI	GAANNNNTTC	1 cut(s)	1263
MseI	TTAA	3 cut(s)	252, 750, 1445
MslI	CAYNNNNRTG	1 cut(s)	1433
MspI	CCGG	3 cut(s)	18, 150, 734
MspR9I	CCNGG	2 cut(s)	368, 945
MvaI	CCWGG	2 cut(s)	368, 945
MvnI	CGCG	2 cut(s)	41, 50
MwoI	GCNNNNNNNGC	6 cut(s)	40, 47, 49, 56, 265, 1520
NcoI	CCATGG	1 cut(s)	780
NdeII	GATC	6 cut(s)	685, 736, 850, 1125, 1372, 1437
NlaIII	CATG	7 cut(s)	203, 424, 557, 784, 1007, 1138, 1462
NlaIV	GGNNCC	6 cut(s)	343, 384, 738, 909, 1426, 1648
NmuCI	GTSAC	1 cut(s)	934
NspI	RCATGY	1 cut(s)	1007
NspV	TTCGAA	1 cut(s)	1098
PaeI	GCATGC	1 cut(s)	1007
PciSI	GCTCTTC	3 cut(s)	41, 50, 59
PcsI	WCGNNNNNNNCGW	1 cut(s)	114
PdmI	GAANNNNTTC	1 cut(s)	1263
PfeI	GAWTC	6 cut(s)	10, 110, 122, 481, 979, 1057
PflFI	GACNNNGTC	1 cut(s)	145
PkrI	GCNGC	4 cut(s)	78, 1111, 1351, 1627
PleI	GAGTC	2 cut(s)	304, 412
PpsI	GAGTC	2 cut(s)	304, 412
PpuMI	RGGWCCY	1 cut(s)	1646
PshAI	GACNNNNGTC	1 cut(s)	293
Psp5II	RGGWCCY	1 cut(s)	1646
Psp6I	CCWGG	2 cut(s)	366, 943
PspFI	CCCAGC	1 cut(s)	5
PspGI	CCWGG	2 cut(s)	366, 943
PspN4I	GGNNCC	6 cut(s)	343, 384, 738, 909, 1426, 1648
PspPI	GGNCC	7 cut(s)	141, 196, 428, 907, 1131, 1294, 1646
PspPPI	RGGWCCY	1 cut(s)	1646
PsuI	RGATCY	2 cut(s)	736, 1125
PsyI	GACNNNGTC	1 cut(s)	145
RseI	CAYNNNNRTG	1 cut(s)	1433
SapI	GCTCTTC	3 cut(s)	41, 50, 59
SaqAI	TTAA	3 cut(s)	252, 750, 1445
SatI	GCNGC	4 cut(s)	77, 1110, 1350, 1626
Sau3AI	GATC	6 cut(s)	685, 736, 850, 1125, 1372, 1437
Sau96I	GGNCC	7 cut(s)	141, 196, 428, 907, 1131, 1294, 1646
SchI	GAGTC	2 cut(s)	304, 413
ScrFI	CCNGG	2 cut(s)	368, 945
SduI	GDGCHC	1 cut(s)	36
SfaNI	GCATC	1 cut(s)	763
SfuI	TTCGAA	1 cut(s)	1098
SinI	GGWCC	5 cut(s)	141, 428, 1131, 1294, 1646
SmiMI	CAYNNNNRTG	1 cut(s)	1433
SmlI	CTYRAG	2 cut(s)	278, 1082
SmoI	CTYRAG	2 cut(s)	278, 1082
SphI	GCATGC	1 cut(s)	1007
Sse9I	AATT	8 cut(s)	302, 594, 678, 729, 786, 1223, 1314, 1368
SsiI	CCGC	1 cut(s)	1506
SspMI	CTAG	1 cut(s)	1269
StyD4I	CCNGG	2 cut(s)	366, 943
StyI	CCWWGG	2 cut(s)	780, 1290
TaaI	ACNGT	3 cut(s)	100, 145, 292
TaiI	ACGT	1 cut(s)	1657
TaqI	TCGA	5 cut(s)	117, 361, 1017, 1077, 1098
TasI	AATT	8 cut(s)	302, 594, 678, 729, 786, 1223, 1314, 1368
TfiI	GAWTC	6 cut(s)	10, 110, 122, 481, 979, 1057
Tru1I	TTAA	3 cut(s)	252, 750, 1445
Tru9I	TTAA	3 cut(s)	252, 750, 1445
TscAI	CASTG	2 cut(s)	1217, 1522
TseFI	GTSAC	1 cut(s)	934
TseI	GCWGC	4 cut(s)	76, 1109, 1349, 1625
Tsp45I	GTSAC	1 cut(s)	934
TspDTI	ATGAA	3 cut(s)	454, 986, 1447
TspRI	CASTG	2 cut(s)	1217, 1522
Tth111I	GACNNNGTC	1 cut(s)	145
VpaK11BI	GGWCC	5 cut(s)	141, 428, 1131, 1294, 1646
XapI	RAATTY	3 cut(s)	729, 1223, 1314
XceI	RCATGY	1 cut(s)	1007
XcmI	CCANNNNNNNNNTGG	1 cut(s)	206
XmnI	GAANNNNTTC	1 cut(s)	1263
XspI	CTAG	1 cut(s)	1269

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.